Human Humalyzer 2000 User manual

Humalyzer

2000

Introduction

Principles

Photometer Temperature

Flowcell Display, System

Troubleshooting..

Power

Photometer

Vacuum Temperature Main Internal

Keypad Display

Serial

Error

nn

of

Operation

...........

contro!

and

Vacuum

Keypad,

Control...

Supply

Pump

Control

PCB..

Printer

... ...

Port

Messages

Table

of

Contents

...

.

Pump

and

Thermal

enteresse

and

Pump

..

Service

..

Printer.....

Control

PCB

...8

1

5

10

11

Manual

Specifications

Replacement

Diagrams...

Block

PCB

Layouts

Main

Pump Interface

Printer

Incubation

Schematics

Main

Pump

Interface

Printer

Incubation

...

Parts

Diagram

PCB

Control

PCB

......

PCB

Control

PCB

...

PCB

....

block

PCB

...

PCB

.

.....

block

PCB...

ns

40

Service

Calibration...

Procedures

Opening

Cleaning

Flowcell Flowcell Flowcell

Valve

Vacuum Lamp

Filter

Calibration

Restore Restore Temperature Absorbance

the

Tubing Adjustment Disassembly

Tubing

Adjustment

replacement.

Replacement

Label

an

Calibration Filter

Calibration....

Instrument....

semen

Flowcell

Replacement..

Labels

Calibration

......

....

..

.

Data

....

..

Replacement

sorbance

Linearity

.

~

...

15

17 17

Rev. B 7/94

Rev.

B

Introduction

The

instrument

wavelengths filters. A removable

built-in

standard. well tube

vacuum

as 1 cm stations.

Layout

Figure 1 shows and

Figure 3 shows

The

interface

a

26-conductor

interface interface

port (DB-25S,

Finally, a 4-conductor

PCB PCB

connector

is a

and

37°C

pump

When

the

square

Both

the

PCB

mounted

ribbon

supplies separate

via a 14-conductor

(DB-9P)

IDC)

connects

general-purpose,

incubation.

flowcell

and

flowcell

cuvettes.

the

exterior-of

the

cable

provides

an

external

is

The

incubation

the

chassis

inside

and

connects

to

the

connects

bichromatic

Two

removed,

instrument

block

instrument,

layout.

the

header. A 2-conductor

power

ribbon

to

the

interface

the

photometer

additional

extremely

autoclavable

the

and

For

cover

for

the

cable

main

PCB

rapid

instrument

also

contains

the

read

Figure 2 shows

clarity,

behind

internal

terminated

PCB

via a 26-conductor

ready/sample

system

wavelengths

fluid

waste

bottle

accepts

well

some

cabling

the

front

cable

printer.

with

with a 4-conductor

PCB

are

sampling

with

standard

an

incubation

are

temperature

the

inside arrangements

panel,

running

The

printer

IDC

ribbon

to

the

optional,

connects

interface

with

six

with

level

12

block

of

from

PCB

14-pin

cable.

cable

available

for a total

low

carryover.

sensing

mm

controlled

the

DIP

are

round

with

cover

are

not

to

the

main

connects

plugs.

The

parallel

and

header.

12

to

assembly,

main

PCB

PCB.

of

eight

A

supplied

tubes

as

round

37°C.

shown.

PCB

to

the

to

the

The

serial

port

1

by

All

AC

AWG

itself

is

mains

wires

feed

tied

directly

Hazardous

beneath

(high

115VAC

AC

voltage)

with

to

this

ground.

line

the

incubation

power

circuitry

is

ground

voltages

block.

cable

before

enclosed

to

the

incubation

WARNING

are

present

Always

servicing

behind

the

behind

disconnect

the

AC

cover.

block

heater

the

AC

instrument.

However,

PCB.

cover

the

external

Note

and

three

that

the

18

block

：

i

Humalyzer

2000

Service

Manual

Wi

Front

Incubation

Display

panel

Rear

dress

panel

Printer

paper

Printer

roll

cover

Rev:

B

Sensor

ー

*

jack

Vacuum fitting (blue)

Waste

«.

fitting

(white)

M

indicator

Vacuum

fitting

Parallel

Bottle

SP

port

/

Fan

outlet

Serial

Port

(blue)

|

Power

inlet

Exhaust filter

—__

Power

switch

—

g

Voltage

select switch

Z

Waste

fitting

(white)

Sensor wires

$

へ

Sensor

lead

Figure

*

Humalyzer

1.

Instrument

2000

Service

Exterior

Manual

ἐν

Κον.

Β

Display

ha

\

—

A

\

a

Printer

Г)

&

PCB

|

Interface

o

=

ーー

ES

PCB

\

|

l

N

\

~

|

>

=

Keypad

cables

Cable

main

to

PCB

P

PCB.

Cover (raised)

Transformer

=

Incubation

trol.

Main

PCB

Vacuum

pump

Cover

Figure

Humalyzer

È

screw

3.

Chassis

2000

Service

\

Photometer

Layout

Manual

4

Principles

of

Photometer

The

light

from a tungsten-halogen

the

sample

filter

wavelengths, filter peak

voltage compares at

the

photodetector

phase

cap

(RC

end

absorbance

it

wheel

passes

the

output

of

the

decay)

(offset).

contains.

and

photodiode. The

and

is

speed

in

front

of

produced

sampled

of the

comparator

output

comparator

determines

calibration

Operation

lamp

The

sealed box

controlled

the

photodetector,

by

the

photodetector.

voltage

is

proportional

output

to

enables a 16

is

the

base

(gain).

on

filter

wheel

to

the

output

to

the

proportional

of

the

Another

is

passed

the

approximately 3 rps

an

intensity

log (10

potentiometer

horizontally

opposite

contains

infrared The

voltage

of

an

exponential

bit

counter

of

to

the

for

side

of

eight

optical

is

then

in

an

the

light,

absorbance.

absorbance)

is

though

the

interference

under

switch

capacitor

8254

used

the

read

well

filters

software

triggers a sampling

fed

to a comparator

decay

programmable

whereas

The

and

to

the

resistance

is

adjust

tube

or

flowcell

and

contains a rotating

of

various

control.

circuit.

width

used

the

high-end

timer.

across

to

adjust

As

each of

which

The

of the

the

pulse

positive

log

Rev.

low-

B

=

Temperature

The

incubation

(025*C):

oscillator centered-at drive

the,

this

signal

Power

resistors

opto-triac.

employed

located

on

“Flowcell

An

integral

means pinch to wires

PCB

of a solid-state

valve

the

flowcell.

built

to

indicate a full

These

U14A.

+2.5

CLK2

at

2.08

A red

for

the

cell

and

DC

is

opened

into

control

block

and

thermistors

The

waveform

V.

Schmitt

input

of

kHz,

the

located

LED

read

on

located

well

PCB.

Vacuum

vacuum

Sample

the

pump vacuum for a precise

(or

bottle

condition

read

well

are

maintained

are

multiplexed

at

U14A

triggers

U4.

37°C

the

except

U11

The

microprocessor

set

point.

bottom

on

the

that

of

block

driver

Pump

maintains a constant

air)

cap

sensor

are

interval

is

drawn

used

on

the

circuit

in

waste

at

0.5

second

is a sawtooth

convert

the

through conjunction

this

heats

incubation

PCB

illuminates

U10

applies

vacuum

on

the

pump

under

microprocessor

the

flowcell

bottle.

37°C

by

means

rate

into

the

of

approximately

to a square

the

block

12VDC

control

with a detector

wave

system

are

switched

when

heat

on

an

external PCB . A control,

and

into

to

(raw)

circuit

of

thermistors

feedback

1.2

for

current

maintain

is

on. A similar

across

to

the

waste

path

Vpp

amplitude

reversal

the

to

115VAC

heater

waste solenoid-operated

apply

on

the

bottle,

bottle.

(10k

of

and

frequency

via

system

resistors

by

this

vacuum

Sensor

pump

control

an

to

of

is

.

..

]

“Display,

A

24x

Keypad,

2:line

membrane

interface

cover

PCB

and

are

character

switch

keypads

via a 14-pin

connected

and

Liquid

are

DIP

the

to

Thermal

Crystal sealed

cable.

interface

Display

behind

The

PCB.

Humalyzer

Printer

(LCD)

the

front

tails

of

the

printer

The

2000

Service

is

mounted

panel

Keypad

assembly,

Manual

inside

overlay.

switch

The

layers

consisting

the

LCD

are

cover.

Two 4 x

connects

fed

a

of

4

to

the

through

the

20-column

Rev.

B

5

thermal connected

System

The

27256

incorporating a real-time

also Almost

intervals

printer,

to

the

interface

Control

instrument

EPROM. A battery-backed

maintains

all

and

is

based

the

digital

I/O

pulse

date

timer.

printer

PCB

on

clock

and

is

accomplished

widths

are

PCB,

and

metal

via a 14-pin

the 8 bit

Z80

microprocessor.

non-volatile

is

used

store

time. A static

with

measured

using

supports,

DIP

cable.

RAM

data

RAM

chip

an

8255

the

is

mounted

The

(random

such

as

calibration,

(6264)

Programmable

counter

is

channels

to

the

software

access

used

memory)

test

for Peripheral of

an

rear

dress

is

permanently

chip

setups,

temporary

Interface.

8254

programmable

panel

and

stored

(MK48T08)

samples,

storage.

Time

is

and

in

a

6

Troubleshooting

The

instrument

section,

each

outlined.

incorporates

subassembly

or

solid-state

component

electronics

group

in

a-very

is

discussed,

reliable

possible

field-proven

problems

design.

and

solutions

In

Rev.

B

this

are

Power

The

power

voltage bridge +15

VDC

diode

regulates

A

"dead"

on

the

voltage. power

rear

panel

fuse

holder

fuse

puller,

Use

only

If

voltage

present,

Supply

supply

supplied

BR2.

The

and

-15

bridge

main

cable

BR1,

this

down

instrument

PCB

If

there

and

lift mounted or

carefully

the

recommended

is

present

the

diode

consists

to

the

center

VDC

which

to

may

or

transformer.

is

no

open

the

at

bridge

of a transformer,

transformer

tap

is

for

analog

provides

+5

VDC

be

just a blown

voltage the

instrument.

AC

cover

on

the

inside

pry

it fuse.

J6,

check

BR1

is

converted

used

as a reference circuitry.

+12VDC

for

the

logic

Also,

present

out

or

at

Remove

off

towards

of

the

with a small

Do

not

for

input

BR2

may

two

diode

to

for

Another

(raw,

and

other

fuse.

However, a blown

the

voltage

J6,

check

the

front

rear

panel.

screwdriver.

substitute

voltage

have

failed.

bridges,

36

VAC,

regulators

transformer

11-13

VDC

low

select

the two

other

on

switch

fuse.

#4

screws

of

the

Locate

rating!

VR3, VR4,

and

three

center

voltage

tapped,

VR4

and

output

nominal)

control

fuse

setting

To

replace

holding

instrument.

the

blown

Install a 0.6A

Replace

and

VRS.

regulators.

which

VRS,

supplies

to

the

circuits.

may

must

the

fuse,

the

Locate

fuse

and

3AG

the

If

feeds

which

11

system,

indicate a problem

match

disconnect

AC

cover

the

remove

slow

AC

cover.

no

voltage

Line

diode

provide

VAC

and

the

line

to

double

it

blow

is

to

VR3

the

clip

with

fuse.

a

Photometer

The

photometer assembly

.

Read

.

Lamp

.

Photometer

*

Filter

The

read

well

fixed

plate

and a spring

tube

or

flowcell

the

cell

heat

PCB. A stainless

spring

The

life

inactivity. replacement

shock

and

plate

lamp

of

the

to

lens

into

is

tungsten-halogen,

instrument,

However,

as

the

instrument

brackets

well

assembly

and

brackets

PCB

wheel

assembly

the

and

is

detected

the

light

since

under

output

simply

can

be

and

motor.

serves

loaded

by

to

plate.

an

steel

path

between

rated

the

lamp

heavy

becomes

may

necessitate

hold

the

examined

cell

heat

hold

the

tube

Mechanical

opto

pair

plunger

the

at

6V,

is

turned

usage

low

the

near

lamp

and

as

several

component

PCB

or

flowcell

adjustments

(phototransistor

in

the

bottom

opto

pair.

10W,

and

off

automatically

lamp

remains

the

end

replacement

lens

in

alignment

is

intended

of

its

centered

and

center

on

service

due

systems:

are

not

IR

LED)

of

to

after

for

longer

life.

to

filament

with

the

on

the

cell

required.

mounted

the

cell

block

be

maintenance

30

minutes

periods

In

addition, a physical

breakage.

rest

of

block,

The

between

presence

at

the

forces a small

free

of

instrument

and

may

The

the

assembly.

a

of

ends

of

for the

require

lamp

The

a

Humalyzer

2000

Service

Manual

Rev.

B

'

7

brackets

be

completely.

ENTER. wavelength, volts. measure

regulator

lamp.

The Because attempt

The

neoprene contained

maintenance

extreme

may

low,

provide

centered

The

If

all

the

circuitry

Refer

photometer

the

to

modify

filter

wheel

belt.

in

humidity

be

compromised.

the

filter(s)

adjustments

on

the

light

path.

To

check

instrument

position,

or

most

voltage

to

the

PCB

photodetector

turns

The

the

wheel

free

for

and

of the

at

the

on

the

section

contains a photodiode, a very sensitive

the

photometer

on a shoulder

filter

wheel

are

for the

or

may

life

temperature

Run

be

in

both

The

low

lamp

prints

the

voltages

main

“Lamp

suspect.

the

voltage

lamp

terminals.

PCB

and

of

metal

of

the

are

test # 186

horizontal

lamp

must

be

output,

detected

are

(Q6

Replacement”.

PCB.

screw

requires

deposition

instrument.

encountered,

Refer

run

voltage

for

each

filter.

low,

the

If

the

and

VR1)

circuitry

shaft

no

type

as

described

to

the

service

lamp

is

However,

and

vertical

replaced

test

lamp

may

solid-state,

and

adjustments.

construction

optical

if

output

#186.

is

above.

Press

for

each

The

voltages

is

the

likely

voltage

be

the

driven

in a non-ideal

characteristics

If

procedure

dimensions,

becomes

MENU,

filter.

The

printout

should

suspect.

is

much

problem.

op

amp

it

should

by

the

The

and

only

“Filter

lower

circuit

require

filter

optical

are

intended

environment

one

or

Replacement”.

so

that the

low

then

be

between 2 and

As a final

than

Otherwise,

and

wheel

interference

on

one

two

filter

filament

or

the

type

186

shows

4.5

phototransistors.

no

service.

motor

to

be

or

more

voltages

lamp

and the

check,

VDC,

replace

Do

via

a

filters

where

filters

can

fails

press

10

the

not

are

Flowcell

If

proper

liquids

The

flowcell

for

cleaning

Replacement"

Erratic caused section coupling

readings by

Valve

The

valve

the

pinch vacuum the

valve is

clogged.

care

is

taken

have been

should

tubing

"Flowcell

tubes.

line

is

has

and

parts

and

(excessive

improper

Ensure

uses a short

valve

tubing

from

operating,

See

the

to

clean

sampled,

be

become

“Flowcell

installation

Tubing

the

section

the

cleaned

damaged

replacement.

dither)

Replacement’.

that a leak-free

length

may

be

Luer

fitting.

but

the

“Valve

the

flowcell

regularly

Refer

Disassembly”.

may

of,

or

of

silicone

blocked.

Press

flowcell

Tubing

between

itself

as

or

the

to

be a result

failure

Check

seal

tubing.

To

PURGE

is

clogged.

Replacement”.

uses,

should

described

flowcell

the

of

is

made

check

require

is

service

of

the

flowcell

the

insertion and

If

the

for

and

If

you

especially

little

in

"Cleaning

clogged,

procedures

trapped

tubing

that

valve

operates

clogged

listen

for

do

not

after

or

no

the

flowcell

“Flowcell

air

in

the

flowcell.

or

coupling

depth

no

of

the

tubes

are

but

valve

tubing,

aspiration.

hear

aspiration,

highly

proteinaceous

maintenance.

Flowcell".

can

flowcell

kinked

If

the

be

disassembled

Tubing

This

can

tubes.

tubes

or

pinched

|

no

sample

unscrew

If

you

hear

the

is

the

valve

be

See

the

into

the

off.

drawn

aspiration,

up,

flowcell

tubing

Humalyzer

2000

Service

Manual

8

Rev.

B

Vacuum

The runs fittings. only

at a higher

very Adjustment”.

than

the

event

section

Pump

vacuum

continuously,

until

high

usual,

pump

Check

that the

finger-tight.

or

lower

altitudes,

If

but

otherwise

that

the

"Exhaust

"Temperature

The

temperature

recalibration.

“Temperature

In

Calibration”.

and

pump

you

probably tubing

Do

not

level

than

the

vacuum

the

vacuum

works),

exhaust

Filter

filter

Replacement".

Control

control

the

system

event

calibration

Pump

control

is over-tighten

the

trimpot

pump

gets

is

Control

PCB

have a leak

firmly seated

instrument,

is

taking

the

exhaust

wet

very

must

should

in

on

the

such

may

require

longer

filter

due

to a waste

stable

and

be

verified

PCB

require

the

vacuum

barbs

plastic

Luer

as

on a shelf,

adjustment.

to

achieve

is

likely

bottle

ordinarily

or

no

maintenance.

side.

Check

and

all

fittings

fittings!

clogged

spill,

does

re-established,

or

See

full

vacuum

not

If

if

and

it

must

If

the

waste

are

tight.

the

waste

the

instrument

the

section

(runs

should

be

replaced.

require

adjustment

refer

to

the

vacuum

bottle

Turn

bottle

“Vacuum

much

be

replaced.

the

section

cap

the

is

located

is

used

longer

See

or

pump

and

fittings

at

In

the

Humalyzer

2000

Service

Manual

Rev.

B 9

Main

Swat

PCB

Under failures

factory

of

point

normal

authorized

vital

signals.

waveforms

are

circumstances,

highly

unlikely, technicians.

The

instrument

are

shown

there

but

if

There

can

in

Figure

are

they

occur,

are ten

be

observed

4.

no

adjustments

it

is

test

in

to

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Humalyzer

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11

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Humalyzer

2000

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12

Rev.

B

Error

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Humalyzer

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B

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to

the

inside

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cover

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the

at

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screw.

to

the

Prop

Humalyzer

2000

Service

Manual

Rev.

B

15

Cleaning

The overnight,

clogging

accurate,

the

To

一

the

flowcell

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of

the

repeatable

flowcell,

clean

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Purge

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flowcell

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4.

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5.

Purge

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should

it

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be

of

shift,

flowcell

results.

is

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air

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for 3 minutes.

15

ml

0.1N

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air

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tubing

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for

at

least 5 seconds.

ml

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distilled

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hydroxide

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least 5 seconds.

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storing

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flowcell.

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its

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(NaOH

).

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the

flowcell

6.

7.

8.

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minutes.

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seen

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flowcell

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at

least

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to

into

the

15

ml

be

removed

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acid

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purge

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the

to

air

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more

filled

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the

with

fluid

flowcell

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be

water.

for

Humalyzer

2000

Service

Manual

16

Rev.

B

Sample

tube

Bulkhead

W

Cell

Screws

Cell

Figure

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a

п

o

insert

window

insert

5-A.

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f

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set

_

=

tube

tubes

screw

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Sample

tube Exit

Sar

\

x

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/

tube

Figure

5-C.

Humalyzer

Tubing

2000

Service

orientation

Manuâl

Human Humalyzer 2000 User manual

Specifications and Main Features

Frequently Asked Questions

User Manual