Human Elisys Uno User manual

E

lisys Uno

| User Manual

|

Cat.No. 1

735

0/1

Revision List of the Manual

No.

Rev./DATE

REVISION DESCRIPTION

01

01/2006

-06First edition

02

02/2008

-02Adaptation to new corporate design

0303/2009

-05Performance

check kit added

a

NOTICE

Analytical instruments for in vitro diagnostic application involve the handling of human samples and controls

which should be considered at least potentially infectious. Therefore every part and accessory of the respective

instrument which may have

come into contact with such samples must equally be considered as potentially

infectious.

BIOHAZARD

The „BIOHAZARD“ warning label must be affixed to instrument prior to first use with biological material !

Servicing Note:

Before doing any servicing

on the instrument it is very important to thoroughly disinfect all possibly contaminated

parts. Before the instrument is removed from the laboratory for disposal or servicing, it must be decontaminated.

Decontamination should be performed by authorised wel

l-trained personnel only, observing all necessary safety

precautions. Instruments to be returned have to be accompanied by a decontamination certificate completed by

the responsible laboratory manager. If a decontamination certificate is not supplied, the

returning laboratory will

be responsible for charges resulting from non

-

acceptance of the instrument by the servicing centre, or from

authority’s interventions.

HUMAN

Gesellschaft für Biochemica und Diagnostica mbH

| Max

-

Planck

-

Ring 21 · 65205 Wiesba

den · Germany

| Tel.: +49 61 22/99 88

-

0 · Fax: +49 61 22/99 88

-

100

| e-Mail: human@human.de ·

www.human.de

b

I

1

INTRODUCTION

This manual is considered as a part of the instrument; it has to be at the operator’s hand as well as at the

maintenance operator’s availability. For accurate installation, use and maintenance, please read the following

instructions carefully.

In order to avoid instrument or personal damages, carefully read the ”GENERAL SAFETY

WARNINGS”, describing the suitable operating procedures. In case of breakdowns or any troubles with the

instrument, apply to the local Technical Service.

2

USER WARRANTY

HUMAN warrants that instruments sold by one of its authorised representatives shall be free of any defect in

material or workmanship, provided that this warranty shall apply only to defects which become apparent within

one year from the date of delivery of

the new instrument to the purchaser.

The HUMAN representative shall replace or repair any defective item at no charge, except for transportation

expenses to the point of repair.

This warranty excludes the HUMAN representative from liability to replace any

item considered as expendable in

the course of normal usage, e.g.: lamps, valves, syringes, glassware, fuses, diskettes, tubing etc.

The HUMAN representative shall be relieved of any liability under this warranty if the product is not used in

accordance w

ith the manufacturer's instructions, altered in any way not specified by HUMAN, not regularly

maintained, used with equipment not approved by HUMAN or used for purposes for which it was not designed.

HUMAN shall be relieved of any obligation under this war

ranty, unless a completed installation / warranty

registration form is received by HUMAN within 15 days of installation of this product.

This warranty does not apply to damages incurred in shipment of goods. Any damage so incurred shall be re

-

ported

to th

e freight carrier for settlement or claim.

3

INTENDED USE OF THE INSTRUMENT

[IVD]

The instrument has to be used for the expected purposes and in perfect technical conditions, by qualified

personnel, in working conditions and maintenance operations as descri

bed in this manual, according to the

GENERAL SAFETY WARNINGS. This manual contains instructions for professional qualified operators.

4

GENERAL SAFETY WARNINGS

Use only chemical reagents and accessories specified and supplied by HUMAN and/or mentioned in

this manual.

Place the product so that it has proper ventilation.

The instrument should be installed on a stationary flat working surface, free from vibrations.

Do not operate in area with excessive dust.

Work at room temperature and humidity, according to

the specifications listed in this manual.

Do not operate this instrument with covers and panels removed.

Only use the power cord specified for this product, with the grounding conductor of the power cord connected to

earth ground.

Use only the fuse type a

nd rating specified by the manufacturer for this instrument, use of fuses with improper

ratings may pose electrical and fire hazards.

To avoid fire or shock hazard, observe all ratings and markings on the instrument.

Do not power the instrument in potenti

ally explosive environment or at risk of fire.

Prior to cleaning and/or maintaining the instrument, switch off the instrument and remove the power cord.

For cleaning use only materials specified in this manual, otherwise parts may become damaged.

It is rec

ommended always to wear protective apparel and eye protection while using this instrument.

Respective warning symbols, if appearing in this manual, should be carefully considered.

II

5

DISPOSAL MANAGEMENT CONCEPT

The currently valid local regulations governin

g disposal must be observed. It is in the responsibility of the user to

arrange proper disposal of the individual components.

All parts which may comprise potentially infectious materials have to be disinfected by suitable validated

procedures (autoclavin

g, chemical treatment) prior to disposal. Applicable local regulations for disposal have to be

carefully observed.

The Instruments and electronic accessories (without batteries, power packs etc.) must be disposed of according to

the regulations for the di

sposal of electronic components.

Batteries, power packs and similar power source have to be dismounted from electric/electronic parts and disposed

off in accordance with applicable local regulations.

6

INSTRUMENT DISINFECTION

Analytical instruments for in

vitro diagnostic involve the handling of human samples and controls which should be

considered at least potentially infectious. Therefore every part and accessory of the respective instrument which

may have come into contact with such samples must equally

be considered as potentially infectious.

Before doing any servicing on the instrument it is very important to thoroughly disinfect all possibly contaminated

parts. Before the instrument is removed from the laboratory for disposal or servicing, it must be

d

econtaminated/disinfected. Decontamination/disinfection should be performed by a authorised well

-

trained

personnel, observing all necessary safety precautions. Instruments to be returned have to be accompanied by a

disinfection certificate completed by the

responsible laboratory manager. If a disinfection certificate is not

supplied, the returning laboratory will be responsible for charges resulting from non

-

acceptance of the instrument

by the servicing centre, or from authority’s interventions.

7

NOTICE

Eve

ry effort has been made to avoid errors in text and diagrams, however, HUMAN GmbH assumes no

responsibility for any errors which may appear in this publication. It is the policy of HUMAN GmbH to improve

products as new techniques and components become avai

lable. HUMAN GmbH therefore has to reserve the right

to change specifications if necessary in the course of such improvements.

III

IV

Contents

1

Introduction

................................

...11.1

Intended Use

................................

...........................

1

1.2

Warning Markings

................................

................

2

1.2.1

Safety Symbols

................................

.............

2

1.3

Safety Precautions

................................

................

3

1.4

Operating Precautions

................................

........52

Installation

................................

.....72.1

Instrument Setup

................................

..................

7

2.2

Instrument Check Out

................................

......

11

3

Principles and S

pecifications

................................

13

3.1

Technical Specifications

................................

...144

ELISYS UNO Manager

................................

..............

17

4.1

Opening the Software and Security

................................

............

17

4.1.1

Opening the ELISYS UNO Program

................................

.....174.1.2

Password Security and Logging In

................................

......174.1.3

Security Menu Options

................................

...........................

18

4.2Alignment Setup

................................

.................

19

4.2.1

Alignment

................................

....................

19

4.2.2

Rack2 Dispense Height

................................

...........................

25

4.2.3

Filter Voltages

................................

............

26

4.2.4

Channel Blanks

................................

..........274.2.5

Display Instrument Parameters

................................

..........294.2.6

Restore Instrument Parameters

................................

..........294.2.7

ELISYS UNO Manager Toolbar Icons

................................

..294.3

ELISYS UNO Manager Tabs

................................

.............................

30

4.3.1

Layout

Tab

................................

...................

30

4.3.2

Sample Tab

................................

.................

34

4.3.3

Test List Tab

................................

................

35

4.3.4

Report Tab

................................

...................

36

4.4

Main Menu Selections and Features

................................

...........

39

4.4.1

Management Menu

................................

39

4.4.2

Routines Menu

................................

..........424.4.3

Lot # Registration

................................

.....434.4.4

QC Tracking

................................

.................

43

4.4.5

Sample DB

................................

...................

44

4.4.6

Settings

................................

........................

44

4.4.7

Secu

rity

................................

.........................

44

4.4.8

Help

................................

45

5

Running ELISYS UNO

................................

...............

51

5.1

General Settings

................................

.................

51

5.1.1

Auto Wash

................................

...................

51

ii/iv

Human

Elisys Uno User Manual

5.1.2

Software Settings

................................

.....525.2

Start of Day

................................

...........................

57

5.2.1

Run Start of Day

................................

........575.2.2

Sample Database Setup

................................

.........................

59

5.2.3

Calibrators

................................

...................

65

5.3

Running Samples

................................

................

706Assay Editor

................................

756.1

Main Menu and Toolbar

................................

..776.1.1

Assay

................................

..............................

77

6.1.2

View

................................

...............................

77

6.1.3

Substances

................................

..................

78

6.1.4

Panels

................................

............................

79

6.1.5

Indices

................................

...........................

79

6.1.6

Security

................................

.........................

81

6.1.7

Settings

................................

........................

82

6.1.8

Icons

................................

...............................

83

6.2

Creating ELISA Assays

................................

.......846.3

Assay Steps

................................

...........................

88

6.3.1

Select Step

................................

...................

88

6.3.2

Edit Step

................................

.......................

96

6.3.3Arrow Buttons

................................

...........

96

6.3.4

Delete Step

................................

..................

97

6.3.5

Copy Step

................................

.....................

97

6.4

Interpretation Settings

................................

.....986.5

Special Groups Assay Editor

................................

.........................

100

6.6

Standards

................................

............................

103

6.7

Blank

................................

......

106

6.8

Controls

................................

1077Enzyme Immuno Assay Editor

................................

...........................

113

7.1

Assay Editor and ELISAs

................................

..

113

7.1.1

ELISA Specifi

c Modes

................................

.............................

114

7.2

Assay

-

Select Steps

-

................................

.......

117

7.3

QC Criteria

................................

...........................

1188ELISYS UNO Manager and ELISAs

................................

......................

121

8.1

Running ELISAs

................................

..................

121

8.2

Accepting and Adjusting Standard Curves

................................

.............................

1259Troubleshooting

................................

......................

133

9.1

Flags and Error Messages

................................

..............................

133

9.1.1

Flags

................................

.............................

133

9.1.2

Error Mes

sages

................................

........

135

iii/iviv/ivHuman

Elisys Uno User Manual

1/1

38

1

Introduction

ELISYS UNO

is a computer controlled instrument system, capable of automating any or all stages of ELISA assay

processing that involve these functions

:



Fluid Handling

aspirates and dispenses from 5 µL to 1.95 ml



Incubating

heating temperature

s-coil heats to 37°C, plate heats to 25°C or 37°C,

or no temperature control (ambient).



Mixing

reaction plate only



Timing

from 1 second to 24 hours



Optical Reading

UV/visible range



Calculating

uses numerous pre

-

programmed equations



Data Storage

unlimit

ed capacity



Data Reporting

many options and customisations to choose from

The system allows one to define and program an unlimited number of customised protocols by selecting displayed

menu options from a Microsoft Windows® software program (see Operatin

g Precautions, Section 1.4).

ELISYS UNO

may also be used in production processes involving micro volume dispensing, diluting, incubating, and

reading.

ELISYS UNO

is a unique automatic system. Reactions occur in standard plastic microwells Reagent bottles

and

sample tubes are placed into the removable instrument racks. The instrument is programmed to pick up from one

place, dispense to another, wash the probe, read

the wells, incubate, mix, etc.

1.1

Intended Use

FOR IN

-

VITRO DIAGNOSTIC USE

This instrument i

s designed for use in processing ELISA tests for in vitro diagnostic application. It is an instrument

intended to be used by trained laboratory professionals who are capable of selecting the appropriate features and

options for each specific cli

nical appli

cation.

2/138Human

Elisys Uno User Manual

1.2

Warning Markings

1.2.1

Safety Symbols

Symbols t

hat may appear on the product:

WARNING

Protective Ground

CAUTION

BIOHAZARD

Risk of Shock

(Earth) Terminal

Refer To Manual

Risk of Infection

FUSE: For continued protection against ris

k of fire, replace with fuse only with one of the specified type and current

ratings. Disconnect equipment from supply before replacing fuse.

These terms may appear on the product:

DANGER

indicates an injury immediately accessible as you read

this marki

ng

WARNING

indicates an injury hazard not immediately accessible as

you read this marking

CAUTION

indicates a hazard to property, including the product

These terms may appear in this manual:

WARNING

WARNING statements identify conditions or practices

that could result in injury or loss of life. WARNING

indicates an injury hazard not immediately accessible as

you read this marking.

CAUTION

CAUTION statements identify conditions or practices

that could result in damage to this product or other

property.

3/1

38

1.3

Safety Precautions

To assure operator safety and prolong the life of your instrument, carefully follow all instructions outlined below.

-

Read Instructions

Please take the time to read this manual carefully before using this instrument. Review the

follo

wing safety precautions to avoid injury and prevent damage to this instrument or any products connected

to it. To avoid potential hazards, use this instrument only as specified. For best results, familiarise yourself

with the instrument and its capabilitie

s before attempting any clinical diagnostic tests. Refer any questions to

your instrument service provider.

-

Servicing

There are no user

-

serviceable parts inside the instrument. Refer servicing to qualified service personnel.

Use only factory

-

authorised pa

rts. Failure to do so may void the warranty.

-

Wear Protective Apparel

Many diagnostic assays utilise materials that are potential biohazards. Always wear

protective apparel and eye protection while using this instrument. Always operate this instrument with

the

aerosol shield lowered

.

-

Follow Operating Instructions

Do not use this instrument in a manner not specified by the manual, or the

protection provided by the instrument may be impaired.

-

Use Proper Power

Cord Use only the power cord specified for this

product and certified for the country of use.

-

Ground the Product

This product is grounded through the grounding conductor of the power cord. To avoid

electric shock, the grounding conductor must be connected to earth ground. An optional method is to a

ttach a

ground strap from the external grounding terminal on the rear panel of the instrument to a suitable ground

such as a grounded pipe or some metal surface to earth ground.

-

Observe All Terminal Ratings

To avoid fire or shock hazard, observe all rat

ings and markings on the instrument.

Consult this manual for further ratings information before making connections to the instrument.

-

Install as Directed

Install the instrument on a sturdy, level surface capable of safely supporting the instrument’s

we

ight (45 kg). The mounting surface should be free of vibrations. The instrument does not require fastening

to the bench top

.

-

Provide Proper Ventilation

Refer to the installation instructions for details on installing the product so it has

proper venti

lation. The instrument should be surrounded by the following clearances: 46cm on each side,

117cm on top, 15

cm in front, and 18cm in back.

-

Do Not Operate Without Protective Covers

Do not operate this instrument with covers and panels removed.

-

Do Not O

perate Without Probe Shield

Do not operate this instrument with shield removed. Doing so risks the

operator to biohazard injury from the probe

.

-

Use Proper Fuse

Use only the fuse type and rating specified by the manufacturer for this instrument.

-

Avoid

Exposed Circuitry

Do not touch exposed connections and components when power is present.

-

Avoid Excessive Dust

Do not operate in an area with excessive dust

.

-

Do Not Operate With Suspected Failures

If you suspect there is damage to this instrument,

have it inspected by

a qualified service person.

-

Do Not O

perate in Wet/Damp Conditions

-

Do Not Ope

rate In An Explosive Atmosphere

Keep Instrument Surfaces Clean and Dry

Solvents such as acetone or thinner will damage the instrument. Do not

use solvent

s to clean the unit. Avoid abrasive cleaners; the aerosol shield is liquid

-

resistant, but easily scratched.

Clean the exterior of the instrument with a soft cloth using plain water. If needed, a mild all

-

purpose or

4/138Human

Elisys Uno User Manual

nonabrasive cleaner may be used. Use

as a disinfectant a 10% solution of chlorine bleach (5.25% Sodium

Hypochlorite) or 70% isopropyl alcohol. Take special care not to spill liquid inside the instrument.

5/1

38

1.4

Operating Precautions

WARNING:

Insufficient RAM will adversely affect the performance

of your instrument. Therefore, if using

Windows® ME, the minimum RAM required is 96 MB. For Windows® XP, the minimum RAM required is 128 MB.

-

Watch the instrument during "Start of Day" operation to ensure that the probe and wash head dispense

functions are

operating properly.

-

Be sure to run a sufficient number of controls in each assay. If controls are not within their acceptable limits; or if

incomplete, or non

-

uniform washing is suspected, disregard test results.

-

Since the ambient light may interfere with

the optical sensors used to monitor mechanical movements, always

operate the instrument with the top cover down.

-

Do not operate the instrument if the probe is damaged or the pressure is unstable.

-

Do not operate the instrument if the wash head probes are d

amaged.

-

If the Waste bottle is overturned during operation, set it upright. When the run has finished, check that the filter

has not become wet, and replace it if necessary. If the hydrophobic filter becomes wet due to an overturned

Waste bottle, it will b

e blocked.

Continued use of the instrument with a blocked filter will impair washer

effectiveness and may result in damage to the instrument.

-

Do not fill reagent bottles past the neck. Doing so may cause the system to inadvertently aspirate air.

-

Do not fil

l Wash or Rinse bottles into the neck to prevent fluid from entering the pressure tube.

-

The Wash and Rinse bottles are pressurised during normal operation, and the Waste bottle is under vacuum.

-

Do not remove bottle caps or tubing connections while the bo

ttles are pressurised/evacuated.

-

Turn the instrument off or click the Pause Engine in the Management menu before adding more solution,

changing bottles, or connecting tubing.

The quality of washing often affects the validity of test results. To assure ad

equate washing follow these

precautions:

-

Perform “End of Day” to clean probe with bleach and flush wash head with H2O

-

Handle and store the wash head carefully to prevent damage.

-

Use the prime cycle before each wash.

6/138Human

Elisys Uno User Manual

Notes:

7/1

38

2

Installation

How to unpack, set up, and check out your ELISYS UNO instrument.

ELISYS UNO

is carefully packaged in a custom

-

made container to assure its safe arrival. If upon receipt the outer

packaging is damaged report damage to your freight carrier imm

ediately.

When shipping, it is important that the instrument be anchored and packaged in the original manner to prevent

shipping damage.

Therefore, retain all shipping screws and packaging in the event the instrument requires future

relocation

. The detail

s how to unpack the instrument once it is removed from its crate can be found in the

Unpacking Instructions Manual (Cat. No. 17350/4).

2.1

Instrument Setup

1.

Connect the drain tube to the drain tube connector on the bottom of the instrument by pushing the

tubi

ng over the fitting on the connector.

2.

Place the other end of the drain tube into a drain container (at least 2L

–

not included). Next, the bottle

must be placed at a level below the instrument, either by using an access hole on the lab bench, or by

routing

the tubing to the front or the back of the instrument as desired. The drain line may also be

connected to an approved permanent drain.

CAUTION: Do not allow the end of the tubing to rest in the drain container below the expected level of the waste

liquid

.3.Match the colo

ur-

coded connectors on the three bottle cap assemblies to the colo

u

red connectors on the

right side of the instrument. Turn each connector about 1/4 turn clockwise to lock it in place. Insert the

sensor cable jacks, matching the colo

u

red t

ie wrap with the colo

ur-

coded connectors.

Figure 2.1

-

1 Connectors

CAUTION: The hydrophobic filter on the waste bottle is designed to protect the pump from liquid and may become

clogged when wet. Arrange the tubing so that the filter hangs down below the

connecter on the side of the

ELISYS

UNO to prevent clogging.

8/138Human

Elisys Uno User Manual

4.

Put de

-

ionised water into the bottle marked Rinse. Put wash buffer provided into the bottle marked Wash

for ELISA tests. Leave the Waste bottle empty. Check that each bottle cap is securely f

astened and that no

sensor wires are crossed. Note that the Waste bottle has short sensor leads in order to detect when waste

is nearly full. Rinse and Wash bottles have long leads in order to sense when bottles are nearly empty.

5.

Fill the Prime bottle

with fresh, clean de

-

Ionised water. This should be done each day

-

this water enters

the precision calibrated syringe pump and therefore must be very pure to avoid damage and prolong the

life of these components. Prime bottle installed as shown in

Figure 2

.1-2.Figure 2.1

-

2 Install Prime Bottle

6.

Using

the serial cable provided, connect the computer’s serial port to the

ELISYS UNO

serial port (reference

Figure 2.

1-3 Back of Instrument).

If necessary, purchase a USB to serial converter, available thro

ugh other

sources.

Figure 2.1

-

3 Back of Instrument

Power

Switch

Power Cord

Connection

Serial Port

Reset Button

9/1

38

7.

Turn on the computer and insert the installation CD. The installer should automatically start, if not, select

“Run” from the Windows® Start Menu, run CD ROM drive:

\setup, and follow the prompts for installing

the program. Run the

ELISYS UNO

program after installation. Enter

Admin

as the user ID and

Admin

as the

password. (Reference

Figures 2.

1-4 through 2.

1-9

below.)

Figure 2.1

-

4 Double

-

Click to Begin Setup

Figure 2.1

-

5 Welcome to ELISYS UNO 6.2

Figure 2.1

-

6 Choose Destination Folder

Figure 2.1

-

7 Finish Installation

Figure 2.1

-

8 Icons Appear on Desktop

Figure 2.1

-

9 Admin Login

8.

Connect the

power cord to the instrument, then to an approved power source. It is strongly advised that a

UPS (Uninterruptible Power Supply) be used to avoid power interruptions to the

ELISYS UNO

and to the

computer.

10/138Human

Elisys Uno User Manual

9.

Place a rack onto each of the two rack holders. T

he default positioning places the Reagent rack on the left

and the Sample rack on the right. Place the plate loaded with microwells into the plate carrier at the right.

10.

The program default is to use COM1 for communication with the instrument. If connectin

g the

instrument to a different port, go to the Settings Menu and select Software. Select a communications port

and click “OK”.

Figure 2.1

-

10 Software Settings

The Default Mode is

greyed

out if working with an instrument with no washe

r. (Reference

Figure 2.1

-10)

11/1

38

2.2

Instrument Check Out

With the instrument connected to a computer, start the

ELISYS UNO

software, and switch on the instrument.

When the instrument powers up, note the following actions:

-

All racks move to the front (home) posi

tions-The probe moves to its home position (to the left), over the plate, and then to the wash cup

-

The syringe pump primes

-

The instrument and the optical system lights come on

These events are controlled by firmware installed in your instrument however, t

he software must be running for

proper operation.

If the instrument’s power comes on, but these actions do not occur and the beep sound continues, there is a

problem with the communications setup. Check the serial cable connections and COM port settings.

For the Instrument with Washer:

At this time, the wash arm is raised from its shipping position. Install the wash head (found in the accessories

carton) to the wash arm using the two attached thumbscrews. The luer fittings must face outward (toward the

u

ser), and the colour

-

coded fittings on the tubing should be fastened finger

-

tight to the wash head. Refer to

Figure

2.2-

1 Wash Head Installation.

Figure 2.2

-

1 Wash Head Installation

12/138Human

Elisys Uno User Manual

13/1

38

3

Principles and Specifications

The syringe pump is used to make preci

se dilutions. The syringe measures volumes of 2.5 ml or less. The single

probe moves left and right as well as vertically. It is equipped with a liquid surface detection mechanism that stops

the probe automatically when the tip is sufficiently submerged.

Probe washing uses de

-

ionised H2O from the

prime bottle and drains to the bottle below.

Each of the two racks and the plate move independently toward the front and back of the instrument. Commonly

referred to as a

r

eagent rack, a Sample rack, and a React

ion plate. However, reagents can be placed in the Sample

rack, or two racks can be used to perform pre

-

dilutions. Each rack has an arrangement of holes or grooves

configured to hold different types of tubes, bottles, micro tubes, microwells, and other con

tainers. Racks are

identified in the software in order to tell the instrument which configuration is to be used. They are also displayed

graphically.

The incubator plate/well can be set to heat to 25°C, 37°C, or remain at ambient room temperature. The

plate/well

will heat to 25°C providing the ambient room temperature is below 25°C. (It should be noted that the option of

heating the plate/well to 25°C should only be used when the ambient room temperature is consistently below

20°C.)

When the probe ca

rries a reagent to an incubated reaction plate, the temperature

-

controlled coil can be set to pre

-

warm the liquid before dispensing.

Reagent racks can be loaded and unloaded with bottles from run to run. The location of each reagent is indicated

using a c

olour

-

coded computer screen. Alternately, preferred reagent rack setups can be stored in panels. For

convenience, multiple pre

-

loaded racks can be stored in the refrigerator ready to load and use.

When taking an optical reading, the reaction plate automa

tically positions itself under the 4

-

channel optical

system. Four lamps are aligned to simultaneously shine down through four wells. A filter wheel with eight filters

rotates constantly below the plate. The filter wheel is designed so that four filters

align with the four lit wells for

absorbance readings.

Depending on the setup, reports may be displayed or printed to create permanent lab records and physician

reports.

14/138Human

Elisys Uno User Manual

3.1

Technical Specifications

Overall:

Dimensions

86cm W x 51cm L x 40cm H, approx. weig

ht = 35kg

-

Reagent and Sample Dispensing:

Capabilities:

Dilutions, predilutions, single or multiple reagents

Pumps:

One syringe pump, sized: 2.5 ml

Probe:

316 stainless steel for maximum reagent compatibility, level

sensing

Minimum and Maximum Volume:

5 µl–1.95 ml

Maximum number of specimens:

96 (including calibrators and controls)

Maximum number of reagents:

Typically 27 or 44

o

Assorted replaceable racks and custom designed racks

are available for various bottle sizes.

o

Reagents can also be programmed

to go to the Sample

rack.

Reaction vessel:

Standard microwells, strips

Instrument bottles:

1L Priming bottle, 2L Wash bottle w/ low volume warning

sensor,1L Rinse bottle (or 2nd wash) w/ low volume

warning sensor

-

Incubation, timing and temperature cont

rol:

Thermal control:

Plate/Well 25°C, 37°C, or ambient temperature

o

Temperature controlled to 25°C providing the ambient

room temperature is below 25°C

o

Sample Rack is not temperature controlled.

-

Washing

Wash Head:

8-probe, automatic prime and rinse

Programs:

Create and run user programmable protocols (aspirate,

dispense, soak). Can wash wells for re

-

use as applicable

-

Reading:

Optical design:

Reads absorbance in four simultaneous channels; NIST

traceable calibration; user selects monochromatic or

bi

chromatic results

Light Source:

Tungsten

-

Xenon lamp

4 position filter wheel:

405, 450, 490, 630

Interference filters:

Long life, hard coat, ion

-

assisted deposition, +/

-

2nm, 10nm

typical half

band pass

Linear range:

-

0.2 to 3.0A

Photometric Accuracy

:

± (1% of the reading +0.005A from 0 to 1.5A)

± (2% of the reading +0.005A from 1.5 to 3.0A)

15/1

38

-

Software:

Format:

CD-Rom and Internet upgrades

Operating Systems:

Windows® 98, 2000, ME, NT 4.0, or XP

Minimum System:

Pentium®/133 MHz, 64 MB RAM, SVGA moni

tor, Serial port

or USB port with Serial adapter

Recommended system:

PentiumII®/333 MHz, 128 MB RAM, CD drive, Windows® 98

or above

Secondary menu options:

Create/edit protocols, import/export data, etc., Control,

Run, Setup

Calculation modes:

Absorbance,

single standard, factor, fixed time kinetics,

kinetics by standard or factor, multi

-

calibrator point

-to-

point, linear regressions, log

-

logit, cubic spline, and

nonlinear regressions (curve fit).

Self monitoring modes:

Lamp, bottle volume, filters, pressure

, vacuum, mechanical

function, and more

QC options:

Store control data, print Levey

-

Jennings or QC range plots,

calculate SDs

Serial port:

RS232 output only, 19200 Baud,1 start bit, 8 data, 1 stop,

no parity, no handshake, Serial cable provided

-

Power:

Vol

tage Range:

100-250VAC

Frequency Range:

50-60Hz

Power Maximum:

160W

-

Environmental Conditions for Safe Operation:

Mains supply voltage:

Fluctuations not to exceed ±10% of the nominal voltage

Altitude:

Up to 2000 m

Humidity:

80% for temperatures up to 31°C

decreasing linearly to 50%

humidity at 40°C

Temperature:

5°C to 40°C (See NOTE)

-

Recommended Environmental Conditions:

Operating Temperature:

18-35°C recommended

Operating Humi

dity:

Less than 85% recommended

16/138Human

Elisys Uno User Manual

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38

4

ELISYS UNO Manager

4.1

Opening the Software and Security

ELISYS UNO uses the standard Windows® controls, windows, and dialogs. Refer to your Windows® documentation

to become familiar with these controls and how to use them

4.1.1

Opening the ELISYS UNO Program

-

Pow

er on the instrument.

-

Double

-

click on the

ELISYS UNO

icontoopen the

ELISYS UNO

Manager

software.

NOTE: It is not necessary to turn the instrument off when restarting the software.

4.1.2

Password Security and Logging In

-

When either the

ELISYS UNO

Manager or A

ssay Editor software are opened for the first time, a User name

and a

Password must be entered.

-

For User name, type

Admin

-

For Password, type

Admin

There are three security access levels: Administrator, Manager and Operator. View

Figure 4.1.2

-1for secur

ity levels.

Security Level

Administrator

Manager

Operator

Disable Security

YNN

Enable Security

YNN

Create Manager

YNN

Create Operator

YYN

Remove Manager

YNN

Remove Operator

YYN

Change Password

YYY

View Log File

YNN

Figure 4.1.

2-1 Security Levels

Human Elisys Uno User manual

Specifications and Main Features

Frequently Asked Questions

User Manual