potential data of
standard or internal
standard solution is
indicated with ADC
error, insufficient
sample alarm, noise
alarm or level alarm.
(2) In calibration, data
calculation is
disabled.
(The result of calibration
is not updated nor saved
onto FD.)
WARNING <<Photometry assay>>
The factor value 'K'
determined in calibration
(Photometry assay)
(ISE assay)
Same as above
differs from the previous
value by more than ±20%.
<<ISE assay>>
The calibrator
concentration value and
slope value determined in
calibration differ from the
previous values by more
than the compensation
limit (%).
WARNING The mean error determined
in multi-point calibration is
Replace STD and
check again.
larger than the SD limit
(input value).
2 - 13
Page 26
(cont’d)
Alarm
Control
No.
3551 to
3588
Alarm Category
SENSITIVITY?
Subcode
86 1 to 36
(ch.)
Level Description Remedy
WARNING In linear (with 2 to 6 points) or
nonlinear calibration, a
difference between the mean
STD (1) absorbance and the
mean STD (N)
(Note 7 )
The result of
calibration is not
updated nor saved
onto FD.
absorbance is smaller than
the sensitivity limit (input
value).
NOTES :
7.N:=2 for linear
(2points)
=2 to 6 for nonlinear
and linear (3 to 6
points)
(Span point input value)
8.If either STD (1) or STD
(N) alone has been
measured, the
absorbance value of the
other STD is checked
using the previous data.
4151 to
4153
4161 to
4163
ISE LEVEL 90 1 WARNING The mean potential value
(EAV) at three out of five
measurement points of
internal standard solution is
outside the following range.
(Internal standard)
Na:-90.0mV ≤ EAV ≤-10mV
⇒OK
90 2 WARNING K:-90.0mV ≤EAV ≤-10mV
⇒OK
90 3 WARNING Cl:100.0mV ≤EAV ≤
180.0mV
⇒OK
ISE NOISE 91 1 WARNING A difference (FIV) between
maximum and minimum
potential values at three of
five measurement points of
internal standard solution is
outside the following range.
(Internal standard, sample)
Na:0.7mV < FIV(2) - FIV(4)
91 2 WARNING K :1.0mV < FIV(2) - FIV(4) Same as above
(1)Replace STD
and carry out
calibration.
(2)Replace the Na
electrode.
(1)Same as above
(2)Replace the K
electrode.
(1)Same as above
(2)Replace the Cl
electrode.
Carry out reagent
priming and check
for bubble
formation.
91 3 WARNING Cl:0.8mV < FIV(2) - FIV(4) Same as above
2 - 14
Page 27
Alarm
Control
No.
4171 to
4173
AlarmCategory
Subcode
LevelDescriptionRemedy
ISE PREP.921WARNING Upon calibration, the slope
value is within the following
range.
Na : 45.0mV ≤ Slope value
≤ 49.9mV or
68.1mV ≤ Slope value
922WARNING K: 45.0mV ≤ Slope value
≤ 49.9mV or
68.1mV ≤ Slope value
923WARNING Cl : -39.9mV ≤ Slope value
≤ -35.0mV or
-68.1mV ≥ Slope value
(cont’d)
•Make sure that the
standard solution
and reagent are set
properly.
•Make sure that the
standard solution is
free from
concentration or
deterioration.
•Make sure that the
electrodes (Na, K,
Cl) are within their
guaranteed life.
4181 to
4183
4191 to
4193
ISE SLOPE931WARNING (1) In the result of
calibration, the slope
value is within the
following range.
(2) The response
characteristic of
electrode is poor (in case
carry-over rate (A) is as
indicated below).
Na : (1)SLOPE < 45.0mV
(2)0.232 < A
932WARNING K: (1)SLOPE < 45.0mV
(2)0.160 < A
933WARNING Cl : (1)SLOPE < -35.0mV
(2)0.490 < A
ISE I.STD941WARNING The concentration of internal
standard solution (C(IS)) is
within the following range.
Na : C(IS) < 120.0mEq/L or
160.0mEq/L < C(IS)
942WARNING K: C(IS) < 3.0mEq/L or
7.0mEq/L < C(IS)
943WARNING Cl : C(IS) < 80.0mEq/L or
120.0mEq/L < C(IS)
Refer to alarm
codes 92-1 to 3.
Same as above
Same as above
(1) Replace STD
and carry out
calibration
again.
(2) Replace t he
internal
standard
solution.
Same as above
Same as above
2 - 15
Page 28
Alarm
Control
AlarmCategory
No.
4201 to
4203
ISE
REAGENT
SHORT
4211TWLN
TEST?
Subcode
LevelDescriptionRemedy
951Unassigned
(Liquid level detection for
IS/DIL solution)
952Unassigned (Same as
above)
953WARNING The volume of reference
electrode solution is 30 mL
or less.
961 to 36
(ch.)
WARNING (1) when analyzing two
tests at a time, the
assigned method for
the corresponding test
is inadequate.
(2) Assignment of the
corresponding test is
being done or not
being done when
analysis for two tests is
not being made at the
same time.
(3) When analyzing two
tests at the same time,
the analytical
parameters are not
identical.
Sub-codes (1 to 36)
signify the channel
numbers.
(cont’d)
Replace the
reference electrode
solution with new
one.
Check the ISE
reagent volume.
•Start setting in
order from the
tests that are
designated by the
Measured Point of
the first half of the
1 channel 2 Test
Analysis Method (3
Point, 1 Point and
Rate, Rate B)
•When not
analyzing two tests
at the same time,
do not designate
'Two Test Analysis'
for the Analysis
Parameter.
•With the Analysis
Parameter screen
for the applicable
test, unify all the
parameters below
for the
Simultaneous 2
Test Analysis Test.
• Analytical Method
• Reaction Time
• Sample Volume
• Reagent
Pipetting volume
(R1-R3)
• Calibration
Method
• Calibration Point
• Standard solution
volume
• Standard solution
position
2 - 16
Page 29
Alarm
Control
No.
4511 to
4548
4661 to
4698
Alarm Category
CHEMISTRY
PARAMETER?
CALIBRATION
PARAMETER CALIB.
Sub-
code
98 1 to 36
(ch.)
99 1 to 36
(ch.)
Level Description Remedy
WARNING(1)The relationship
between assay code and
Correct the
parameter.
photometric point is
improper.
(2)The assigned
photometric point lags
behind the specified
reaction time.
(Operation is
impossible.)
WARNING (1)The relationship
between assay code and
Check parameter
and input it again.
calibration type is
improper.
(2)Necessary calibration
points for calibration
type are not input.
(3)Necessary standard
positions for calibration
are not input.
(4)The relationship
between calibration type
and calibration method
is improper.
(5)The standard
concentration values are
not set in ascending
order. (Except for STD
(3) and (4) for isozyme)
(6)Concentration value is
not zero when '99' is
entered for POS. of STD
(1).
1.The photometry assay
data in other than
manual mode is
checked.
2.Operation is
unallowable.
3.Check in (4) is made
with regard to
specifications of timeout calibration, lot-to-lot
calibration, bottle-tobottle calibration and
test selecting
information.
(cont’d)
2 - 17
Page 30
(cont’d)
Alarm
Control
No.
4811 to
4848
Alarm Category
VOLUME
CHECK?
Sub-
code
100 1 to 36
(ch.)
Level Description Remedy
WARNING (1)The total reagent
volume up to the last
photometric point is
more than 250 to
500 µL.
(2)The volumes of
reagents 1 to 3 are all
Check parameter.
If the improper
condition indicated
by alarm can be
detected, correction
and reentry are
required.
zero.
(3)The reagent volume
having a timing behind
the reaction time is not
zero.
(4)The total liquid volume
of sample and reagent is
less than 250 µL.
1.When '999' (stirring
only) is specified for
reagent volume, 0 µL is
taken for the volume.
However, when '999' is
specified for the reagent
volume having a timing
behind the reaction time,
an error occurs.
2.Operation is
unallowable.
3.The final liquid volume
is a total volume of
sample and reagent
within 250 to 500 µL.
5261 CMP. TEST 103 1 to 8 WARNING The setting of formula
number corresponding to
the relevant code is
improper.
(1)An unmeasurable test is
specified for
compensation.
(2)A compensated test is
not included in the
formula.
(3)In photometry assay for
compensated test, the
electrolyte parameter is
specified.
2 - 18
Call up calculation
item screen and
check the
compensation
formula on it.
Page 31
Alarm
Control
No.
5271 to
5356
Alarm Category
SERUM
INDEXES?
104 1 to 36
Subcode
(ch.)
Level Description Remedy
WARNING (1)Although the sub-code
corresponds to the
serum index
measurement test, the
rate-A assay is not
assigned.
(2)Although the sub-code
corresponds to the
serum index
measurement test and
sample blank is to be
corrected, reagent 2
discharge is specified.
(Analysis does not start.)
5431 ON BOARD? 106 1 WARNING There is no measurable
channel.
There is no channel for
which necessary reagent
has been prepared.
(Analysis does not start.)
5441 REAG.POS? 107 1 to 38WARNING (1)The reagent position
specified for a
photometry assay is
also specified for other
photometry assay.
(2)The same reagent
position is specified for
both carry-over cleaning
agent and photometry
assay or ISE test.
(Analysis does not
start.)
5481 CLB(IS)
POS.
108 1 WARNING When ISE is provided,
calibrator or control
positions are set at 55 to 57.
(Analysis does not start.)
5511 to
5516
ACI ERROR 114 1 to 6WARNING Barcode IC malfunctions.
1:Unassigned
2:Unassigned
3:Sample
4:Unassigned
5:Unassigned
6:Transfer
(cont’d)
Check parameter
for serum indexes.
Check each
parameter.
Check each
reagent.
Check the position
for control
calibration.
Replace RSDIST
PC board.
2 - 19
Page 32
Alarm
Control
No.
5561 to
5568
5571 to
5578
Alarm Category
FD-WRITE? 119 1 WARNING A hardware error has
119 2 WARNING A hardware error has
119 3 WARNING A hardware error has
119 4 WARNING A hardware error has
119 5 WARNING A hardware error has
119 6 WARNING A hardware error has
119 7 WARNING A hardware error has
119 8 WARNING A hardware error has
FD READ? 120 1 WARNING A hardware error has
120 2 WARNING A hardware error has
120 3 WARNING A hardware error has
120 4 WARNING A hardware error has
Subcode
Level Description Remedy
occurred in writing the
routine sample
measurement data.
occurred in writing the stat
sample measurement data.
occurred in writing the
control sample
measurement data.
occurred in writing the
individual or cumulative
alarm information.
occurred in writing the
parameter data.
occurred in execution of FD
formatting, copying into FD
or FDD cleaning.
occurred in writing the cell
blank data.
occurred in writing the
routine sample test
selecting information.
occurred in reading the
routine sample
measurement data.
occurred in reading the stat
sample measurement data.
occurred in reading the
control sample
measurement data.
occurred in reading the
individual or cumulative
alarm information.
(cont’d)
(1)Clean the FD.
(2)Replace the FD
with a new one.
(3)Replace the FD
drive.
Refer to alarm
category No. 119.
2 - 20
Page 33
Alarm
Control
No.
5571 to
5578
5581 FD NOT
5601 FD
5621 to
5625
Alarm Category
FD READ? 120 5 WARNING A hardware error has
INSERTED
PROTECT
PRINTER 125 1 WARNING Power supply is turned off
Subcode
120 6 WARNING A hardware error has
120 7 WARNING A hardware error has
120 8 WARNING A hardware error has
121 1,2 WARNING System disk is not set in
123 1,2 WARNING A write-protected disk is
125 2 WARNING Paper has run out or the
125 4 WARNING A hardware error has
125 5 WARNING A time-out error of the
Level Description Remedy
occurred in reading the
parameter data.
occurred in reading the
channel assignment.
occurred in reading the cell
blank data.
occurred in reading the
control parameter.
drive 1 or data disk is not
set in drive 2.
inserted.
or the connector is
disconnected.
printer head has risen.
occurred on the printer.
printer has occurred.
(cont’d)
Refer to alarm
category No. 119.
Insert the relevant
disk.
Unprotect the disk.
Check the power
supply or connector.
Set paper or lower
the printer head.
Check the printer
cable.
(1)Check the
printer cable.
(2)Replace the
printer.
2 - 21
Page 34
Alarm
Control
No.
5631 to
5643
Alarm Category
Subcode
Level Description Remedy
SYSTEM I/F 126 1 WARNING A reception time-out error
has occurred.
126 2 WARNING A transmission time-out
error has occurred.
126 3 WARNING A BCC error or checksum
error has occurred.
126 4 WARNING A parity error has occurred.
126 5 WARNING A framing error has
occurred.
126 6 WARNING An overrun error has
occurred.
126 7 WARNING Frame error
126 8 WARNING Text length error
126 9 WARNING Function character error
(cont’d)
(1)Check the cable
of system I/F.
(2)Check the
contents of
communication
trace.
(3)Check the
contents of
communication
by line analyzer.
(4)Check if a
change has
occurred in
system
parameters.
126 10 WARNING Sample information error
126 11 WARNING Test selecting information
error
126 12 WARNING Comment information error
126 13 WARNING Reception cannot continue
up to the end code because
an illegal character is
received from the host.
Example)A null code is
received from
the host.
5681 BATH
EXCHANGE
FAILURE
130 1 WARNING The start key has been
pressed despite failure in
incubation bath water
exchange.
5696 PANEL I/F 140 1 WARNING An error has occurred in
LCD display module
communication.
('Communication error'
appears on the LCD
display.)
5701 REAGENT? 141 1 to 36WARNING Either reagent positions R1
to R3 or reagent volume
alone is "0" (no
specification).
Do incubation bath
water exchange
again.
(1)Check the
communication
cable of LCD.
(2)Replace the
LCD.
Check and correct
the contents of
parameters R1 to
R3.
2 - 22
Page 35
Alarm
Control
No.
5771 CELL C. O. 145 1 to 10WARNING (1)More than 2 types of
5821 ISE C. O. 146 1 to 40WARNING R1 type is not specified for
Alarm Category
Subcode
Level Description Remedy
carry over evasion (cell)
are specified for 1 test.
(2)Sub-codes (1 to 10)
signify the evasion
types.
ISE test of Reagent Probe
Carry Over Evasion.
(cont’d)
Check and correct
the carry over
evasion (cell).
Check and correct
the Reagent Probe
carry over evasion.
2 - 23
Page 36
2.1.1LCD Display Alarm
Output of Boot Error
Boot error is output to the console and the buzzer for small-size automatic analyzer (beeper is
used). Output is issued unconditionally to the console whenever connected. For the small-size
automatic analyzer, on the other hand, either of the destinations shown below is selected
depending on system. For selection method, refer to (3).
(1) Output to Console
Output to the console always uses a log message. This is because output cannot be
issued in the EAT format within a period from system start to EAT task start. Upon output,
a message is sent out line by line by scroll-up method as in the logging message of
VxWorks. Note that a line is fed before and after a message.
Output format is shown below. For contents of output, refer to (4).
(2) Buzzer Output for Small-Size Automatic Analyzer
The kinds of output are the same as the three kinds in the small-size immunological
system. Each output has a distinctive tone at the frequency indicated below.
1)Self Test Error:50 Hz
2)Loading Error:250 Hz
3)OS Initial Error:1 Hz
Sounding:Continuous
(3) Distinction in Error Message Output
For distinction in error output of small-size automatic analyzer, the configuration register of
a flash memory is used.
According to the readout value of this register, output destination is distinguished as shown
below. The value is set by hardware. (No setting is required in software.)
Table 2-1 Flash-Memory Configuration Register
(0xf2000b)
ValueMeaning
0x01Output to small-size automatic analyzer
Bus errorOther
2 - 24
Page 37
(4) Contents of Output
Output for the small-size automatic analyzer comes in 3 kinds described above. So, the
kinds of console output are listed below.
Table 2-2 Kinds of Console Message Output
ErrorOutput Message
Self-test error1. Self Test Error (0xXX)
XX: Self-test error code
Boot error1. Rom Uncompress Error
2. F/D Boot Error (0xXXXXXXXX)
3. FROM Boot Error (0xXXXXXXXX)
XXXXXXXX: Boot error code (Refer to Section 5.)
OS initial error1. OS Initial Error (Vect = 0xXX,PC=0xXXXXXXXX)
Vect = 0xXX: Vector No.
PC = 0xXXXXXXXX: Program counter
Note that EAT output is issued when possible.
1. For items other than 7 and 8 ,
check whether the motor remains
running even after timeout.
NOTE:
If so, it should be identified as a
motor time out error.
2. Operation check method for 2-pitch
returning of sample disk.
3. Item 10 ; Operation check method
for 2-pitch feed of sample disk.
4. Item 9 ; Check at resetting.
2 - 27
Page 40
Notes:
1. In check procedure other than 7 and 8 ,
motor running status is checked after time-out.
When running, a motor time-out error occurs.
2. Operation check procedure at 2-pitch return of
the sample disk.
3. 10 is the operation check procedure at 2-pitch
feed of the sample disk.
4.
9 is the check at resetting.
.
2 - 28
Page 41
2.3Parameter Check
Parameter check is carried out on the channel for which test is selected on the CHANNEL
ASSIGNMENT screen at start of analysis.
2.3.1Processing Flow
(1)At input of START key in STANDBY status.
Twin test
simultaneous analysis
parameter check
Check result?
NG
STOP
Analytical method
OK
check
Calibration parameter Serum index analytical
checkmethod check
Volume checkReagent relationship check
1)
at carry-over cleaning
Upper/lower limit value CH, ACTIVATE check
check
Test-to-test Check result?
compensation check
Analytical mode check
1)
OK
NG
START
STOP
2 - 29
Page 42
2.3.2Details of Parameter Check
Designation
No.
of Check
1Check of
parameters
for twin test
simultaneous
analysis
Details of Check
The following are checked
for measurable (level 1,
Note 1) photometry tests.
(1) In case of twin test
simultaneous analysis,
the opposite test must
be specified properly.
• Two tests must form
a unique pair, and
one of them alone
must designate the
other.
(2) In case of other than
twin test simultaneous
analysis, tests must not
be paired.
(3) In case of twin test
simultaneous analysis,
two tests must be
identical in all of the
following parameters.
• Data mode
• STD POS.
• STD S. VOL
• Assay code
• Reaction time
• Sample volume
• Reagent volume
• Calibration type
• Calibration point
• Span point
Alarm
DisplaySubdivision
TWIN TEST?1 to 37
(CH No.)
Remarks
Details of this check
are given in "Details
of twin test
simultaneous
analysis" in 2.3.3.
In case of twin test
simultaneous
analysis, an alarm
is issued if one of
two tests is not
registered or the
manual mode is
selected.
NOTE:
1. The term
'measurable'
indicates either
of the following
two levels.
Level 1:
Test registered
and manual
mode not
selected
Level 2:
Same as above
and reagent
provided
2Assay code
check
The following are checked
for measurable (level 1)
photometry tests.
(1) Relationship between
assay code and
photometric point
(check of photometric
point input and input
range)
Refer to the analytical
method table in 1.1.1.
CHEM
PARAM?
2 - 30
1 to 37
(CH No.)
Page 43
Designation
No.
of Check
2Assay code
check
3Calibration
parameter
check
Details of Check
(2) Photometric points after
the specified reaction
time must not be set.
Refer to Note 6 in
"analytical method
table" of 1.1.1.
The following are checked
for measurable (level 1)
photometry tests.
(1) Relationship between
assay code and
calibration type
Refer to "relationship
between calibration type
and analytical method"
in 2.1.
(2) Entry must be made for
CALIB. POINTS
necessary for calibration
type.
Refer to "output by each
calibration method and
check table" in 2.7.
(3) Entry must be made for
STD POS. necessary
for calibration.
For STD specified by
measured STD No. in
"output by each
calibration method and
check table" of 2.7, it is
checked whether STD
POS. is input or not.
Alarm
DisplaySubdivision
CHEM
PARAM?
1 to 37
(CH No.)
CLB. PARAM?1 to 37
(CH No.)
(cont’d)
Remarks
NOTES:
1. Checked
against the
specification of
calibration test
selecting
information.
2 - 31
Page 44
Designation
No.
of Check
3Calibration
parameter
check
4Volume
check
Details of Check
(4) The input value for
SPAN POINT must be
normal.
• When calibration
type is LINEAR (2 to
6-point) or LOGITLOG (3P or 4P)
1) The above input
value must not
equal 0.
2) The above input
value must not
exceed that for
CALIB. POINTS.
(5) Relationship between
calibration type and
calibration method
Refer to "output by each
calibration method and
check table" in 2.7.
(Note 2)
(6) STD CONC. must be
set in ascending order.
Check is made only for
necessary STD POS.
Note that isozyme STD
(3) and (4) are
excluded.
"=" is not regarded as in
ascending order.
(7) When '99' is entered for
POS. of STD (1),
CONC. must be 0.
The following are checked
for measurable photometry
tests.
(1) The total liquid volume
up to the final
photometric point must
be 500 µL or less.
(2) At least one of R1 to R3
volumes must not be 0.
(3) The reagent volume
having a timing behind
the reaction time is not 0.
(4) The minimum liquid
volume must be at least
250 µL.
Alarm
DisplaySubdivision
CLB. PARAM?1 to 37
(CH No.)
VOLUME ?1 to 37
(CH No.)
(cont’d)
Remarks
NOTES:
2. Checked
against the
specification of
calibration test
selecting
information.
When reagent
volume = 0, the
relevant reagent is
not used.
2 - 32
Page 45
Designation
No.
of Check
5Test-to-test
compensation check
6Serum index
analytical
method
check
7Cell carry
over
8ISE carry
over
Details of Check
For formula No. where
compensated test is
measurable:
(1) The compensation test
must be measurable.
In addition, when the
compensation test is
already registered as a
compensated test in any
preceding formula No.,
the check result for the
formula No. must be
OK.
(2) The compensated test
must be on the right
side of the formula.
(3) When the compensated
test is a photometry
test, no ISE test must
be registered for the
compensation test.
(1) The assay code for tests
for serum index
measurement must be
RATE-A. This check is
made only when the
tests are measurable.
(2) In the RATE-A test with
serum indexes, no R2
must be specified when
sample blank correction
is carried out.
(1) More than 2 types of
carry over evasion (cell)
are specified for 1 test.
(2) Sub-codes (1 to 10)
signify the evasion
types.
R1 type is not specified for
ISE test of Reagent Probe
Carry Over Evasion.
(cont’d)
Alarm
Remarks
DisplaySubdivision
CMP. TEST?44 to 51
(FORMULA
No.)
This check is not
performed in case
of ORIGINAL ABS.
S. INDEXES?1 to 37
(CH No.)
CELL. C. O.1 to 10Check and correct
the carry over
evasion (cell).
ISE C. O.1 to 40Check and correct
the Reagent Probe
carry over evasion.
2 - 33
Page 46
2.3.3Details of Twin Test Simultaneous Analysis
∇NGNote 2
CH = 1 to 37Assay code P: Designates OK
for twin testopposite test.
Note 1simultaneousQ: Designated by NGNote 2
P.Q
P.Q
P.Q
analysis opposite test. OK
P.Q
Designated
by one test
alone
Check result OK
Identical in
all parameters
with opposite
testNote 3
OK
Not paired
NGNote 2
NGNote 2
OK
NGNote 2
∆
NOTES:1.Unmeasurable channels are excluded.
However, in twin test simultaneous analysis, alarm occurs when either
one of the two tests is unmeasurable.
2.Alarm is registered with a channel No. assigned for subdivision.
3.Refer to "Details of Parameter Check" in 2.3.2.
2 - 34
Page 47
2 - 35
Page 48
2.4.2Data Alarm Code List
No.Data AlarmRemarks
Output StringISE
PRINTERC
R
T
1
ADC abnornal
2
Cell blank abnormal
3
Sample short
4
Reagent short
5
Absorbance over
6
Prozone error
7
Reaction limit over at all
points
8
Reaction limit over except 1
point
9
Reaction limit over except 2
or 3 points
10
Linearity abnormal at 9
points or more
11
Linearity abnormal at 8
points or less
12
Standard 1 absorbance
abnormal
13
Duplicate error
14
STD error
15
Sensitivity error
16
Calibration error
17
SD error
18
Noise error
19
Level error
20
Slope abnormal
21
Internal standard
concentration abnormal
22
Sample value abnormal
23
Test-to-test compensation
error
24
Test-to-test compensation
disabled
25
Calculation test error
26
Overflow
27
Calculation disabled
28
Expected value high limit
over
29
Expected value low limit over
30 Electrode preparationPREP.R
31 SD absorbance over>AMAX>>
ADC?
CELL?
SAMPLE
REAGN
ABS?
∗∗∗∗∗P
LIMT0
LIMT1
LIMT2
LIM.
LIM.8
S1ABS?
DUP
STD?
SENS
CALIB
SD?
NOISE
LEVEL
SLOPE?
I.STD
R.OVER
CMP.T
CMP.T!
CALC?
OVER
???
H
L
A
Q
V
T
Z
P
I
J
K
W
F
N
L
&
C
M
%
0
X
Photometry
Assay
S.
I/FRo
u
t
i
n
e
O
A
O
Q
O
V
O
T
O
Z
O
P
O
I
O
J
O
K
O
W
O
F
H
U
S
Y
B
G
N
L
E
O
D
O
&
C
O
O
O
M
O
%
O
0
X
O O O
S
C
S
R
S
C
S
t
o
T
o
t
o
T
a
n
D
u
a
n
D
t
t
t
t
t
r
i
r
o
n
o
l
e
l
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
•Data may become blank
space.
•Data may become blank
space.
•Prozone value is output to the
printer only when the monitor
is in the real time mode.
∗∗∗∗∗ indicates a prozone
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
value (5-digit).
O
O
O
O
O
O
•Data becomes blank space.
O
•Data becomes blank space
•Data becomes blank space.
•May concur with other alarm.
•May concur with other alarm.
O
•Data becomes blank space.
BM only
NOTES: 1. If any data caused multiple data alarms, the alarm registered first will be output.
2. When [Specify] is entered for measured value space parameter in the start condition screen.
2 - 36
Page 49
2.4.3Data Alarm Codes
No.Data AlarmPrinterS. I/FDescriptionRemedy
ADC abnormalADC?AThe ADC value of main or
sub wavelength (only main
wavelength in single
wavelength photometry) is
zero.
Cell blank
abnormal
Sample shortSAMPLVBefore sample aspiration,
Reagent shortREAGNTBefore reagent aspiration,
CELL?Q(Photometry assay only)
Two or more of the four
passed cell blank values to
be used for CELL BLANK
are abnormal.
Note: Abnormal when the
difference from the
reference value
(value measured
with cell blank
function on the
maintenance
screen) is ± 0.1 Abs
or more.
the presence/absence of
sample is checked and it is
found that the sample is
absent.
the presence/absence of
reagent is checked and it is
found that the reagent is
absent.
• Perform measurement
again.
• Turn off the power switch,
and then turn it on.
• Perform cell blank
measurement once a
week or after
replacement of the light
source lamp or reaction
cuvette.
• Confirm the adequate
volume of HITERGENT.
After taking a proper
measure, exchange
incubation bath water
once.
• Rinse or replace the
reaction cuvette and
perform cell blank
measurement.
• After washing the light
transmitting window in the
incubation bath, perform
cell blank measurement.
• Inject the sample in a
volume of expected
consumption + 50 µL or
more into a standard cup.
• Insert the lead wire.
• Prepare and set reagent
newly.
• Insert the lead wire.
2 - 37
Page 50
No.Data AlarmPrinterS. I/FDescriptionRemedy
Absorbance overABS?Z(Photometry assay)
The absorbance value to be
used for calculation after cell
blank correction exceeds 3.3
Abs. The absorbance is
checked for every
wavelength.
Prozone error
*****P
('*****'
indicates
a
prozone
value.)
P(Photometry assay only)
In 1-point or 2-point assay
with prozone check, the
prozone error is indicated if
'prozone' is detected.
Prozone check is performed
by comparing the prozone
value (hereafter referred to
as PC value) obtained from
calculation mentioned below
with the prozone limit value
preset on the chemistry
parameter screen. For the
lower prozone limit, the
prozone error is indicated if
the PC value is smaller. For
the upper prozone limit, the
error is indicated if the PC
value is larger.
In the event of prozone error,
the relevant PC value is also
printed out (only in real-time
monitor printing mode).
• In 1-point & rate, rate-A
or rate-B assay, check is
not carried out for the
interval to determine the
absorbance change rate
if an ascending reaction
setting is made for that
interval.
• Dilute the sample or
reduce its volume, and
then perform
measurement again.
• Set the sample correctly.
• Prepare the reagent
again.
• Prozone check values
are printed out only in
the real-time monitor
printing mode.
• Delution or retest with
decreased volume is
performed.
• For no-check, enter
‘-32000’ (lower limit) at
“Prozone limit value” on
the parameter setting
menu screen 1.
(cont’d)
2 - 38
Page 51
No.Data AlarmPrinterS. I/FDescriptionRemedy
Ep
Ep
−
Prozone error
*****P
('*****'
indicates
a
prozone
value)
PShown below are the
expressions for calculation of
the PC value. Assuming that
the preset photometric points
are P1 and P2 and the
difference in absorbance
between two wavelengths at
photometric point P is E:
(1)In 1-point assay
PC value =Ep2,p
Ep2,p
2-1
1-1
-k
k: Liquid volume
correction factor
Note that k = 1 when
1 ≤ P1, P2 ≤ 5 or
6 ≤ P1, P2 ≤ 16 or
17 ≤ P1, P2 ≤ 33 or
34 ≤ P1, P2 ≤ 73.
Prozone check is not
carried out if P2 = 0.
(cont’d)
(2)In 2-point assay
PC value =
23
P2' P3
−
EpEp
−
43
P4 P3
−
Note that prozone check is
not carried out if P3 = 0 or
|Ep4 - Ep3| ≤ 100 × 10
- 4
Abs.
When P2' = 0, P2 is used.
In either case of (1) and (2),
prozone check is not made
for STD (1) measurement.
2 - 39
Page 52
No.Data AlarmPrinterS. I/FDescriptionRemedy
Reaction limit
over
(Photometry assay only)
In 2-point rate, 1-point & rate,
rate-A or rate-B assay, the
main wavelength absorbance
at the photometric point to be
used for calculation exceeds
the reaction limit value (value
• This alarm is issued
whenever the input
photometric range
values l and m for
calculation of the
absorbance change rate
do not satisfy l + 2 < m.
obtained after automatic
correction of input reaction
limit value). The error
condition varies depending
All points NG
Only one point
OK
Only two or three
points OK
LIMT 0
LIMT 1
LIMT 2
on the number of photometric
points within the reaction limit
I
range as shown below.
(1) The reaction limit is
exceeded at all points in
the input photometric
J
range.
(2) The reaction limit is
exceeded at the second
and subsequent points
K
excluding the first point.
(3) The reaction limit is
exceeded at the third and
subsequent points
excluding the first two
points, or at the fourth
and subsequent points
excluding the first three
points.
(Caution)
In 2-point rate assay, check
is made using not the input
photometric range values l
and m alone but all
photometric points between
l and m.
• Dilute the sample or
reduce its volume, and
then perform
measurement again.
• Prepare the reagent
again.
• Check for leakage or
clogging of the reagent
pipettor.
• Input correct values on
parameter registration
menu.
(cont’d)
2 - 40
Page 53
No.Data AlarmPrinterS. I/FDescriptionRemedy
Linearity
abnormal
LIN.
Number of
photometric
points in reaction
limit level range ≥
9
(Photometry assay only)
In 1-point & rate, rate-A or
W
rate-B assay, the absorbance
at each photometric point to
be used for calculation of the
absorbance change rate does
not satisfy the following
relational requirement.
• Make sure the sample
does not contain dust,
etc.
• Dilute the sample and
measure it again.
• If the stirring motor does
not rotate normally,
report to the
servicemen.
4 ≤ Number of
photometric
points in reaction
limit level range ≤
8
LIN. 8
∆E1− ∆E
≤ Linearity limit value
F
Where, ∆E: Absorbance
change rate determined from
absorbance at each
photometric point in reaction
limit level range by least
squares method
∆E
b
× 100
• The light source lamp
should not be used
beyond 750 hours.
• Replace the light source
lamp and perform cell
blank measurement.
∆E1: Absorbance change
rate in first half
∆Eb: Absorbance change
rate in second half
Assuming that the number of
photometric points in the
reaction limit level range is N,
∆E1, ∆Eb and linearity limit
value can be represented as
shown below.
LIMIT8 and LIN.LIMIT
values are both fixed.
(Unit: %,
(1) When N ≥ 9
Linearity limit
value LIMIT 8
value: LIN.LIMIT = 10,
LIMIT8 = 30)
(cont’d)
2 - 41
∆E f
∆E
b
Page 54
No.Data AlarmPrinterS. I/FDescriptionRemedy
4 ≤ Number of
photometric
points in reaction
LIN. 8F
(2) When 4 ≤ N ≤ 8
Linearity limit value LIN.
LIMIT
limit level range ≤
8
∆E f
∆E
b
Note that linearity check is
not carried out in the
following cases.
• The number of
photometric points in the
reaction limit level range
is three or less.
• |∆E|≤ 60
(× 10e - 4ABS/min)
• |∆Ef - ∆E
|≤ 60
b
(× 10e - 4ABS/min)
(cont’d)
Standard 1
absorbance
abnormal
S1ABS?H(Photometry assay) (Note 1)
In calibration, the mean value
of two measured absorbance
values of STD (1) is not
within the specified standard
1 absorbance range (input
value).
Value is absorbance with
End-point Assay, in the case
of late assay, is main wave O
length first absorbance.
Duplicate errorDUPU(Photometry assay)
In calibration, this error is
indicated if the difference in
absorbance (or absorbance
change rate) between the first
and second measurements of
STD (i) is larger than the
DUPLICATE LIMIT (input
value).
(i = 1 to N: N indicates the
number of standards.)
• Prepare the standard
properly.
• Set the standard
properly.
• Unless check is desired,
input a value within
- 32000 to 32000 for
"standard 1 absorbance
range" on the parameter
registration menu 1
screen.
• Replace the seal piece
of pipettor.
• Check fastening of
joints.
• Unless check is desired,
input 32000 for
"duplicate limit
absorbance" on the
parameter registration
menu 1 screen.
2 - 42
Page 55
No.Data AlarmPrinterS. I/FDescriptionRemedy
STD errorSTD?S(Photometry assay)
(1) In calibration, any one of
the following alarms is
encountered with the
measured STD
absorbances:
(EXPONENTIAL and
Logit-Log5P), an extreme
value is found.
(4) After execution of non-
linear calibration
(SPLINE), the result of
extreme value/inflection
point check is NG.
• The parameters on the
screen and FD are not
updated.
(cont’d)
(ISE)
Calibration is invalid.
(Any one of the following
alarms is encountered: ADC
abnormal, sample short,
calculation disabled, noise
error and level error.)
2 - 43
Page 56
No.Data AlarmPrinterS. I/FDescriptionRemedy
Sensitivity errorSENSY(Photometry assay)
Sensitivity check is carried out
only for linear (2 to 6-point),
non-linear or isozyme P
calibration.
This error is indicated if the
difference in mean absorbance
between STD (1) and STD (N)
is smaller than SENSITIVITY
LIMIT (input value).
N: Linear (2-point)/
isozyme P calibration ..... 2
Non-linear/linear (3 to 6-
point) calibration ..... 2 to 6
(input value for SPAN
POINT)
Note, however, that N = 2
when CALIB. POINTS = 2 in
non-linear LOGIT-LOG
(3P)/(4P) calibration.
• The parameters on the
screen and FD are not
updated.
• Set the standard
solutions and reagent
properly.
• Prepare the standard
solutions newly.
• Check the sample
syringe for leakage,
clogging, etc.
• Unless check is
desired, input 0 for
"sensitivity limit
absorbance" on the
parameter registration
menu.
(cont’d)
For the mean absorbance of
STD (1) in span calibration, the
following data is used.
Linear ..... Previous S1 ABS
Non-linear ..... Previously
updated mean
absorbance
Sensitivity check is not made
in R.B. calibration.
SD errorSD?G(Photometry assay)
This error is indicated upon
completion of non-linear
calibration or if the SD value in
linear (multi-point) calibration is
larger than SD LIMIT (input
value).
• Set in correct
concentration series.
• Replace the seal piece
of pipettor.
• Check fastening of
joints.
• Unless check is
desired, input 999.9
for "SD limit
absorbance" on the
parameter registration
menu.
2 - 44
Page 57
No.Data AlarmPrinterS. I/FDescriptionRemedy
Calibration errorCALIBB(Photometry assay)
In linear (2 to 6-point) or
isozyme P calibration, the
calibration condition is checked
at calculation of parameter K.
This error is indicated if there is
a difference of ±20% or more
between the current and
previous K values.
• Same as in SENS
• Pay attention to
storage method and
the time period of
placement on the
sample disk.
• If the result is
satisfactory,
operation can be
continued. After
(ISE)
The calibration concentration or
slope level for display does not
satisfy the following expression.
|Previous value - current value|
(Previous value + current value )/2×100% > COMPENSATE LIMIT
Noise errorNOISEN(ISE)
This error is indicated if the
difference between the
maximum and minimum
potentials is within the following
range at three of the five
measuring points for each test
(on internal standard or sample).
measurement,
record parameters
on the maintenance
menu to store the K
value.
• Set reagent and
perform ISE priming
(with internal
standard solution
and diluent) once.
• Make sure O-ring is
fitted to each
electrode and its
holder and it is not
fitted doubly.
After reattaching,
perform ISE priming
(with reference
electrode solution).
• Replace the tube
and perform ISE
priming (with
reference electrode
solution) once.
• Clean the waste
solution drain path.
• Clean the syringe
and plunger and
replace the seal
piece.
(cont’d)
2 - 45
Page 58
No.Data AlarmPrinterS. I/FDescriptionRemedy
Level errorLEVELL(ISE)
This error is indicated if the
mean potential is outside the
following range at three of the
five measuring points for each
test (on internal standard).
•Set reagent and perform
ISE priming (with
reference electrode
solution) twice.
•Replace the electrode
and perform ISE
priming (with reference
Na: -90.0mV ≤ EAV ≤ -10mV
⇒ OK
K : -90.0mV ≤ EAV ≤ -10mV
⇒ OK
Cl : 100.0mV ≤ EAV ≤
180.0mV ⇒ OK
electrode solution)
once.
•Retighten the nipple.
Or after replacing the
rubber packing, perform
ISE priming (with
reference electrode
solution) once.
•Confirm the proper
combination of tube and
reagent.
•Plug in the cord
properly.
Slope abnormalSLOPE?E(ISE)
(1) The slope level for display
is within the following
range.
Na, K : SLOPE < 45.0mV,
Cl: SLOPE > -35.0mV
•Replace the electrode.
•Remove the probe and
correct clogging.
•Set properly.
•Confirm the proper
combination of tube and
reagent.
(2) Electrode response is
degraded.
(Carry-over rate is as
shown below.)
Na : 0.232 < A
K: 0.160 < A
Cl: 0.490 < A
Electrode
preparation
PREP.RUpon calibration, the slope
value is within the following
range.
•Make sure that the
standard solution and
reagent are set properly.
•Make sure that the
Na, K : 45.0mV ≤ Slope value
≤ 49.9mV or
68.1mV ≤ Slope value
Cl: -39.9mV ≤ Slope value
≤ -35.0mV or
-68.1mV ≤ Slope value
standard solution is free
from concentration or
deterioration.
•Make sure that the
electrodes (Na, K, Cl)
are within their
guaranteed life.
(cont’d)
2 - 46
Page 59
No.Data AlarmPrinterS. I/FDescriptionRemedy
Internal standard
concentration
abnormal
I. STDD(ISE)
The internal standard
concentration (C(IS)) is within
the following range.
Na : C(IS) < 120.0mEq/L or
•Confirm monthly flow
path washing.
•Replace the diluent and
internal standard
solution.
160.0mEq/L < C(IS)
K: C(IS) < 3.0mEq/L or
7.0mEq/L < C(IS)
CL : C(IS) < 80.0mEq/L or
120.0mEq/L < C(IS)
Calculation test
error
CALC?
%
Any data alarm other than
shown below is indicated for
the test to be used for
•Check the data alarm
name and take a proper
measure.
calculation.
Calculation disabled, test-to-
test compensation disabled,
expected value over
OverflowOVER
O
Concentration value (or activity
value) cannot be output within
the specified range of digit
•The data is left blank.
•Prepare appropriate
standard solution.
count.
Calculation
disabled
???
X
•In process of calculation, the
denominator becomes zero.
•An overflow occurs in
logarithmic or exponential
calculation.
•In isozyme Q-channel
concentration calculation, the
data alarm of 'calculation
disabled' is indicated for the
isozyme P-channel data or
the isozyme P channel is not
measured.
•The data is left blank.
•Determine the cause of
failure to color
development such as
improper kind of
standard solution, wrong
set position and
clogging of sample
probe.
•Dilute the sample or
reduce its volume and
perform analysis again.
•Calculation for a calculation
test has been attempted with
the data having a data
blanking alarm.
Expected value
over
L
H
The test result is outside the
expected value range (outside
mean value ± 2SD in case of a
control sample).
•Smaller than the lower limit
–
–
value
•Larger than the upper limit
•This alarm is not
indicated for serum
index.
•Correct setting on the
parameter registration
menu 1 screen.
value
(cont’d)
2 - 47
Page 60
No.Data AlarmPrinterS. I/FDescriptionRemedy
Sample value
abnormal
R. OVER
&
(ISE)
The sample concentration
(C(S)) is within the following
range.
Na : C(S) < 10.0mEq/L or
C(S) > 250.0mEq/L
K: C(S) < 1.0mEq/L or
C(S) > 100.0mEq/L
•Dilute the sample and
analyze it again.
•Direct measurement is
impossible. Utilize
standard addition
method, etc.
•Remove the probe and
correct clogging.
Cl : C(S) < 10.0mEq/L or
C(S) < 250.0mEq/L
Test-to-test
compensation
error
CMP.T
C
(1) In test-to-test
compensation calculation,
any data alarm other than
•Check the channel data
used for test-to-test
compensation.
shown below is indicated
for the compensation data.
(2) In isozyme Q-channel
concentration calculation,
any data alarm other than
shown below is indicated
for the isozyme P-channel
concentration.
Calculation disabled, testto-test compensation
disabled, overflow, random
error, systematic error, QC
error, expected value over
Test-to-test
compensation
disabled
CMP.T!
M
(1) In process of calculation for
test-to-test compensation,
the denominator becomes
zero.
(2) The test to be used for test-
to-test compensation is not
measured.
(3) Any test to be used for test-
to-test compensation has
•The data is left blank.
•Make sure masking is
not specified for the test
to be used for
compensation.
•Check the calculation
formula on the
parameter registration
menu 3 screen.
the data alarm of
'calculation disabled' or
'test-to-test compensation
disabled.'
(4) Any compensation test has
the data alarm which
leaves the data blank.
SD absorbance
over
>AMAX
>
At standard concentration of
“∞”, absorbance of sample or
absorbance change rate is over
or the same compared with
presumptive absorbance or
absorbance change rate.
(cont’d)
2 - 48
Page 61
2.4.4ISE Data Alarms
Alarm Output
ISE
PriorityAlarmPrintoutCRTS. I/FRemarks
Alarm on ISE data processing has 2 kinds shown below; calibration alarm and data alarm.
(1) Calibration Alarm
measurement,
this alarm is set
at any of the
data alarms in
(2) (excluding
data alarms 6 to
9).
OO
OO
OO
OO
2 - 49
Page 62
(2) Data Alarm
PriorityAlarmPrintoutCRTS. I/FRemarks
1ADC
abnormal
2Sample shortSAMPLEVV
3Noise errorNOISENN
4Level errorLEVELLL
5Sample value
abnormal
6Calculation
disabled
7Test-to-test
compensation
disabled
8Test-to-test
compensation
error
9OverflowOVEROO
ADC?AA
R.OVER&&
???
CMP.T!MM
CMP.TCC
××
ISE
Processing
N.E.C.RSTD
OOO
OO
Alarm Output
on Operation
Monitor Screen
×
Registration is made on
the operation monitor in
ADC task.
At occurrence of this
alarm, the 'calculation
disabled' alarm is also set
(for making output data
blank).
OOOO
OOOO
O
OO
O
O
O
×××
××
××
Check is not performed in
ISE data processing.
×××
××
ISE
Check
Data
Alarm
O
×
×
O
2 - 50
Page 63
(3) Registration of Data Alarm "sample short"
Measured Sample
Other than STD (1)A
STD (1)A
NOTES:1. A: Pre-detection is not made.
B: Pre-detection is made and at least either one of the following is encountered.
(i)The number of remaining pulses is zero.
(ii) Abnormal fall detection is activated within ±2 mm of the cup bottom level recognized
C: Other than A and B
(Pre-detection refers to liquid level detection till a time point just before sample aspiration
since start of probe fall.)
2.
O: Output
Alarm Issued in Sampling
(Note 1)
1st Time2nd Time
B
C
B
C
Water
discharge
in probe adjustment.
×: Space
O: Issued
3.
×: Not issued
Data Output
(Note 2)
–
–
–
–
–
–
–
×
O
O
×
O
O
O
Data Alarm
(Note 3)
O
O
×
O
O
×
×
2 - 51
Page 64
2.4.5Alarm Check Method
(1)Photometry Assay Calibration Check
(a)SD check of approximate expression
When SD in the difference between the automatically generated calibration curve
and the measured absorbance value in non-linear calibration is larger than "SD limit
absorbance," comment SD? is printed. SD value is printed under the test name in
the result of calibration. Unless check is desired, input 999.9.
(b)Duplication check
In measurement of reagent blank and standard solution, comment DUP is printed
when the difference in absorbance between two measurements is larger than
"duplicate limit absorbance." Unless check is desired, input 32000.
(c)Sensitivity check
When the difference in absorbance between reagent blank (STD1) and standard
solution (having the maximum concentration if there are multiple standard
solutions), comment SENS is printed. Unless check is desired, input 0.
(d)Standard solution 1 absorbance check
When the absorbance of reagent blank (standard solution 1) exceeds "standard 1
absorbance range," comment S1 ABS is printed. In an end-point assay test, the
absorbance value on the left side of calibration result printout is checked and that
on the right side is checked in a rate-assay test. Unless check is desired, input 32000 to 32000.
2 - 52
Page 65
(2)Reaction Limit Level Check
When concentration or enzyme activity is abnormally high in a rate assay test, correct
data is unobtainable because the substrate or coenzyme in reagent is consumed
completely. Therefore, the upper or lower reaction limit absorbance is set for check .
Check is made on the absorbance at the main wavelength alone.
< Relationship between Alarm Name and Photometric Point >
When 4 points or more over the specified photometric range are within the reaction limit,
measurement is carried out normally. In case no point, 1 point or 2 points are within the
reaction limit, a reference value is obtained depending on an absorbance change
between the first two points. In case 3 points are within the reaction limit (with comment
LIMT2), the value obtained depending on an absorbance change among the first three
points is printed as a reference value.
ABS.
Input photometric range
(with comment LIMT0)
ABS.
Input photometric range
(with comment LIMT2)
ABS.
Reaction limit level
Time
Input photometric range
(with comment LIMT1)
ABS.
Time
Input photometric range
(with comment LIMT2)
Fig. 2-1 Reaction Limit Level Check
Time
Reaction limit
level
Time
< Automatic Correction of Reaction Limit Level >
The instrument corrects the input reaction limit level by adding an absorbance value due
to sample turbidity, etc.
Reaction limit level = Input reaction limit absorbance value + (L1 - LB)
L1:Sample absorbance at photometric point 1
LB:Reagent blank absorbance at photometric point 1
When L1 - LB ≤ 0, automatic calibration will not be performed.
2 - 53
Page 66
(a)Electrolyte compensable range check.
When the ratio of change in calibrator concentration or slope value from the
previous one is larger than the input value, comment CALIB is printed. Unless
check is desired, input 200%.
(b)Calibration check
When calibration factor K has changed by 20% or more from the previous value,
comment CALIB is printed (check value is fixed at 20%).
(c)STD check
If any of the following alarms occurs on calibration data, comment STD? is printed.
• ADC abnormal (ADC?)• Reaction limit over (LIMT0, 1, 2)
• Reagent short (REAGN)• Standard 1 absorbance abnormal
(S1ABS?)
• Absorbance over (ABS!)• Calculation disabled (???)
• Prozone error (xxxxxP)
(d)Measure to be taken on printout of comment
When comment STD? or SENS is indicated, calibration curve will not be updated.
Therefore, recalibration is necessary. In case of CALIB or SD!, sample
measurement is allowed after making sure the result of calibration is normal. In this
case, however, the result of calibration will not be saved automatically onto the
floppy disk. Before turning off power supply, parameters should be recorded on the
maintenance screen.
Table 2-1 Handling of Calibration Result with Comment
Printed
Comment
STD?Not updated
SENSNot updated
CALIBUpdated
SD !Updated
Calibration Result
(display on screen)
(previous result remains)
(previous result remains)
(to new result)
(to new result)
Alarm Name on
Operation Monitor Screen
Calibration curve generation disabled
(code 70-1 to 49)
Standard solution sensitivity abnormal
(code 73-1 to46)
Calibration abnormal
(code 70-1 to 49)
Calibration SD abnormal
(code 72-1 to 46)
Automatic Saving onto
Floppy Disk
The reagent blank (S1ABS)
and calibration factor (K) of
the relevant test are not
saved automatically onto
floppy disk.
2 - 54
Page 67
(3) Reaction Linearity Check
∆Af−∆Ab
In a rate assay test, the linearity in absorbance change is checked.
Check value varies with the number of points (N) in photometric range.
Fig. 2-2 Reaction Linearity Check
< When N ≥ 9 >
The difference in absorbance change quantity between the first-half 6 points (5 sections)
and the latter-half 6 points is obtained and then divided with the overall absorbance
change quantity. When the result of this calculation exceeds the limit in linearity check,
comment LIN. is printed together with the result of measurement.
∆
A
× 100 > Linearity limit value.....LIN.
< When 4 ≤ N ≤ 8 >
The difference in absorbance change quantity between the first-half 3 points (2 sections)
and the latter-half 3 points is obtained and then divided with the overall absorbance
change quantity. When the result of this calculation exceeds the limit in linearity check,
comment LIN.8 is printed together with the result of measurement.
9
Af
∆
∆
• ∆Af, ∆Ab and ∆A in the above formula are all converted into absorbance change in a minute
• In the following cases, reaction linearity is not checked.
9
Ab
− ∆
9
A
by the least squares method.
a)The number of photometric points (N) within the reaction limit is 3 or less ("reaction limit
b)Absorbance change in a minute is 60 × 10-4 Abs or less, or |∆Af - ∆Ab| is equal to or
× 100 > Linearity limit value.....LIN.8
over" will occur).
smaller than 60 × 10-4.
2 - 55
Page 68
(4)Prozone Check
In immunological reaction, the absorbance of calibration curve falls at high concentrations
so that correct data is unobtainable (this is called "zone phenomenon or prozone effect").
Therefore, prozone check is performed by the two methods below and a data comment is
indicated when required.
< Antigen Readdition Method >
In 1-point assay, a small amount (approx. 50 µL) of sample containing antigen is readded
for the second reagent (R2 to R4) to check the change in absorbance before and after
addition (the dashed line in Fig. 2-3 indicates that absorbance falls due to excessive
antigen).
< Reaction Rate Ratio Method >
In 2-point assay, check is performed according to the ratio of the initial reaction rate after
addition of antiserum to the mean reaction rate.
Check Abs.
R1
(antiserum)
Rn (antigen)
Time
Fig. 2-3 Antigen Readdition Method
R1
(1st reagent)
Fig. 2-4 Reaction Rate Ratio Method
Rn (nth reagent) Time
2 - 56
Page 69
Absorbance for concentration
calculation
Prozone check value
(PC value)
Antigen Readdition Method
(1-point assay)
Al + A
2
l-1
Al + A
2
l-1
Ax =
Am + A
PC = -k
m-1
2
Reaction Rate Ratio Method
(2-point assay)
Am + A
Ax =-k
2
(Am - An)/(m-n)
PC =× 100
(Ap - An)/(p-n)
m-1
Al + A
2
l-1
< Judgment >
In case of 'limit value - above,' comment xxxP (xxx indicates PC value) is printed when PC
value is larger than the limit value. In case of 'limit value - below,' the comment is printed
when PC value is smaller than the limit value.
• Prozone check will not be performed in the following cases.
(1) In STD (1) measurement (2) |Ap - Am| < 100 × 10-4 Abs
(Any of the following alarms has occurred; ADC abnormal, sample short,
calculation disabled, noise error and level error.)
Slope abnormal(1)The slope for display is within the following range.
Na, K :Slope < 45.0 mV
Cl:Slope > -35.0 mV
(2)Electrode response is degraded (carry-over ratio is as given below).
Na:0.232 < A
K:0.160 < A
Cl:0.490 < A
ISE prepare(1)The slope for display is within the following range.
Na, K :45.0 mV ≤ Slope ≤ 49.9 mV or
68.1 mV ≤ Slope
Cl:-39.9 mV ≤ Slope ≤ -35.0 mV or
-68.1 mV ≥ Slope
(2)Electrode response is degraded (carry-over ratio is as given below).
Na:0.154 < A
K:0.107 < A
Cl:0.330 < A
Internal standard
concentration
abnormal
Calibration
abnormal
The concentration of internal standard solution (C(IS)) is within the following
range.
Na:C (IS) < 120.0 mEq/L or 160.0 mEq/L < C (IS)
K:C (IS) < 3.0 mEq/L or 7.0 mEq/L < C (IS)
Cl:C (IS) < 80.0 mEq/L or 120.0 mEq/L < C (IS)
On each of the calibrator concentration and slope for display, the previous and
current values are compared and the result exceeds the COMPENSATE LIMIT
value.
Previous value - Current value
× 100 (%) > COMPENSATE Limit
(Previous value + Current value)/2
2 - 58
Page 71
(6)ISE Data Alarm Check
Alarm NameAlarm Check Method
ADC abnormalThe result of ADC is abnormal. (Checked in ADC task)
Sample shortSample volume is inadequate. (Checked in control task)
Noise errorThis alarm is issued when a difference between maximum and minimum
potential values at three out of five measurement points in each test is within
the following range (on internal standard and sample).
NOTE: For CALIB and SD errors, "∗" is output on logging for discrimination.
2 - 63
Page 76
2.5Retry Code Table
CodeDescriptionRemarks
Allowable
Retry Count
1Alarm fuse blown1/50 ms
212 V for lamp1/4.5 secCheck is not made when lamp is turned
off (during initialization, water exchange
or sleep).
315 V for CPU rack1/50 ms
4-15 V for CPU rack1/50 ms
14Liquid in vacuum tank1/4.5 sec
15Vacuum pressure abnormal1/4.5 secCheck is not made within 10 sec after
vacuum pump turns on.
16Waste solution tank1/4.5 sec
17Incubation bath water inadequate100/4.5 secChecked in the following statuses;
standby, operation, sampling stop.
18Distilled water short1) 10/6 secWater supply
2) 50/6 secWarning
3) 150/6 secSTOP
19Distilled water sensor abnormal1/6 secCheck is not made during initialization,
water exchange or wake-up.
31GPCNT110Only at power-on
32GPCNT210Only at power-on
33GPCNT310Only at power-on
34GPCNT410Only at power-on
35GPCNT510Only at power-on
36GPCNT610Only at power-on
37GPCNT710Only at power-on
38GPCNT810Only at power-on
46GMCNT110Only at power-on
47GMCNT210Only at power-on
48GMCNT310Only at power-on
49
50ADC controller1Only at power-on
71Checksum error detected in operation
unit
72Checksum error detected in analyzing
unit
102
103
104
105
106
107
1
1
2 - 64
Page 77
2.5.1Logging Program List
No.FunctionOutput MethodProcessing
1Monitor printoutReal time printout: Specify "print" on
the start condition menu screen.
Printout is made when the specified
values in all tests for one sample are
calculated during operation.
Batch printout: Specify sample
number on the data monitor screen.
2Calibration result
printout
3Remaining
reagent volume
printout
4Cell blank printoutSpecify "cell blank" on the
5Reproducibility
check printout
6Cumulative
mechanism
information
printout
7Parameter printout Specify "parameter" on the
8Program check
printout
9ISE check printoutSpecify "ISE" check on the mechanism
10Photometer check
printout
11Alarm trace
printout (DAILY)
12Alarm trace
printout
(CUMULATIVE)
13Original Abs.
printout
14Communication
trace printout
15Work sheet
printout
16Barcode Reader
check
Printout is made when "calibration" is
selected on the start condition menu
screen and the result of calibration is
output.
Specify "remaining reagent volume
printout" on the start condition menu
screen.
maintenance screen.
Specify "reproducibility check" on the
maintenance screen.
Specify "cumulative mechanism
information" on the mechanism check
menu screen.
maintenance screen.
Specify "program check" on the
mechanism check menu screen.
check menu screen.
Specify "photometer check" on the
maintenance screen.
Specify "alarm trace information" on
the mechanism check menu screen.
Specify "alarm trace information" on
the mechanism check menu screen.
Printout is made when "original Abs." is
specified on the system parameter
screen and operation is executed.
Specify "communication trace printout"
on the mechanism check menu screen.
Specify "work sheet printout" on the TS
registration screen of routine analysis.
Specify "Barcode Reader" check on
the mechanism check menu screen
Real time
and batch
Real time
Batch
Batch
Batch
Batch
Batch
Batch
Real time
Batch
Batch
Batch
Real time
Batch
Batch
Real time
Printout Stop
by Stop Key
O
(In batch mode
alone)
×
×
O
O
×
O
×
×
O
(Note 1)
O
O
×
(Note 2)
O
O
×
NOTES:1. The stop key is invalid during printout.
2. The stop key need be pressed to stop analysis.
(1) Initialization of Printer
2 - 65
Page 78
Effected when power supply is turned on or there is a printer alarm at start of printing
one processing.
(2) Paper Feed
• At end of printout
At the end of each printout (in batch mode of No. 1 and in Nos. 3 to 15), paper is
fed by 3 lines.
(Paper is also fed when printout is stopped by stop key input.)
(3) Page Length
Page length is not determined.
(4) Printing Performance
4 lines/sec (80 characters/sec), 2.54 cm/6 lines, paper feed in pitches of 4.23 mm/line
(1/6 inch)
(5) Printing System
Numerals are right-justified with space at leading positions unless there is a
comment.
(6) Printout with No Blank Space
• Channel number is not registered in keyed-in information.
• Measured data is unavailable.
(7) For Leaving Blank Space
Overflow has occurred at the location where measured data is to be printed.
2 - 66
Page 79
2.6Daily Alarm Trace
(1)Outline
The alarm and retry data from power-on to present time point are printed when "daily" in
"alarm trace data" is specified on the mechanism check menu screen.
(2)Details of Printout
(a) Title
The title "Daily Alarm Trace," date and time are printed.
The printing order of year, month and day depends on the date printout order
specification.
(b) Time (24-hour base)
Time when alarm and retry data has been saved onto FD.
(c) Instrument status
Numerical value representing the instrument status. For details, refer to Table
2-2.
(d) Occurrence count
Indicates how many times the same alarm/retry data as that in a single cycle has
occurred successively (in the number of cycles within 1 to 999).
(e) Identification character
'A' is printed out for alarm data, and 'R' for retry data. Printout is not made when
there is no data in each case.
(f)Alarm data
The alarm data is printed out in the order of main alarm code (3-digit integer), sub
alarm code (3-digit integer) and time of occurrence up to 10 kinds. If 10 kinds are
exceeded, excess ones are not stored. However, they are saved as cumulative data.
Time of occurrence: Time on the basis of operation cycle (000 to 179) when the
alarm has occurred (in 100 msec).
(g) Retry data
The retry data is printed out in the order of retry code (3-digit integer), retry count (3digit integer) and time of occurrence up to 10 kinds. If 10 kinds are exceeded, excess
ones are not stored. However, they are saved as cumulative data.
(h) Key operation data
The key operation data is printed out in the order of key operation code (3-digit
integer), key operation count (3-digit integer) and time of key operation (3-digit
integer). Printout is made only once.
2 - 67
Page 80
Table 2-2 Instrument Status Code Table
Blank row is the unassigned one.
CodeInstrument StatusCodeInstrument StatusCodeInstrument Status
1Initialization2345Incubation bath water
exchange
2Standby
(including parameter check)
3Preparation for operation2547Probe position adjustment
1941ISE priming (REF)63
204264
2143Cell blank65Mechanism resetting during
224466
2446Resetting
(sample probe rotation)
(sample probe up/down)
(reagent probe rotation)
(reagent probe up/down)
(stirrer)
electromotive force
measurement)
(restart unallowable)
operation
2 - 68
Page 81
(3)Printout System
(a) Data is printed out in the sequential order starting from the newest data.
(b) When the alarm/retry data in a single cycle does not reach 10 kinds, it is printed out
closely with no blank line left.
(c) When there is no alarm data or retry data, its title alone is printed.
(4)Notes
(a) Printout can be stopped in steps of cycle by stop key input.
(b) Up to 500-cycle data starting from the oldest one is saved onto FD. However, if the
same data has recurred in consecutive cycles, storage is made as one-cycle data.
(c) In case of FD error, processing is terminated with the title alone printed.
2.6.1Cumulative Alarm Trace
(1)Outline
The alarm data and retry data saved in the FD are printed when "cumulative" in "alarm
trace data" is specified on the mechanism check menu screen.
(2)Details of Printout
(a) Title
The title "Cumul. Alarm Trace," date and time are printed.
The printing order of year, month and day depends on the date printout order
specification.
(b) Final alarm occurrence date/time
The printing order of occurrence date depends on the date printout order
specification. Time printout is fixed to 24-hour base.
(c) Identification character
'A' is printed out for alarm data, 'R' for retry data and 'K' for key operation. Printout is
not made when there is no data in each case.
(d) Alarm data
The alarms, which have occurred in a day, are printed out in the order of main alarm
code (3-digit integer), sub alarm code (3-digit integer) and occurrence count (3-digit
integer) up to 20 kinds.
(Occurrence count: The cumulative number of identical alarms which have occurred
in a day)
(e) Retry data
The retries, which have occurred in a day, are printed out in the order of retry code
(3-digit integer) and retry count (3-digit integer) up to 20 kinds.
2 - 69
Page 82
(f)Key operation data
The key operation data is printed out in the order of key operation code (3-digit
integer), key operation count (3-digit integer) and time of key operation (3-digit
integer). Printout is made up to 16 kinds.
(3)Printout System
(a) Data is printed out in the sequential order starting from the newest data.
(b) When the alarm/retry data in a day does not reach 20 kinds, it is printed out closely
with no blank line left.
(c) When there is no alarm data or retry data, its title alone is printed.
(4)Notes
(a) Printout can be stopped in steps of day by stop key input.
(b) The data obtained from power-on to power-off is taken as daily data and the data for
up to 256 days is saved onto FD starting from the newest data. In case of continuous
energization, however, a day's data is automatically closed when the day changes
and subsequent data is treated as for the following day.
(c) In case of FD error, processing is terminated with the title alone printed.
2 - 70
Page 83
Alarm Trace Printout (DAILY)
Instrument status
Occurrence count
Time of occurrence
Retry count (or sub alarm code)
Retry code (or main alarm code)
Alarm Trace Printout (CUMULATIVE)
Main
code
Occurrence count
Sub code
Retry circuit
Retry code
2 - 71
Page 84
2.6.2Parameter Code List
2 - 72
Page 85
(1)Parameter Printout
Table 2-3 Contents of Printout
Numerical
Input from
Screen
0Title
1 to 37Photometry assay parameter
38 to 40ISE parameter
998calculation tests
8 compensation tests
Control positions (1 to 5)
Serum indexes
Printing order
Printing format (when with card printer)
Set test
Carry-over evasion
System parameter
DIP switch data
Contents of PrintoutWithout ISEWith ISERemarks
OO
OO
×
O
(× for ISE-
related tests)
( O: Printed×: Not printed )
O
O
(2)Program Check Printout
Program version No.
2 - 73
Page 86
2.6.3Communication Trace
(1)Outline
The contents of communication between the analyzer and external system are printed
according to the specification of "communication trace printout" on the mechanism check
menu screen.
(2)Printing Items and Their Contents
Details are given in Table 2-4.
Table 2-4 Printing Items and Their Contents
Printing ItemContents
TitleTitle "Communication Trace," date and time are printed.
The printing order of year, month and day depends on the
date printout order specification.
TimeCommunication start time – Communication end time
(hour, minute, second) (hour, minute, second)
Communication modeTransmission
Reception
Error messageRefer to Table 2-5.
Example of communication textRefer to the system I/F specification.
AU → HOST
HOST → AU
2 - 74
Page 87
(3)Printout System
In printout of
(a) Printout order
Sequential printout starting from the latest communication
(b) Presence/absence of error message and printout system
Details are given in Table 2-5.
Table 2-5 Presence/Absence of Error Message and Printout System
Error
Message
NoneBoth transmission
BCC SUM
PARITY
FRAMING
OVER RUN
FRAME
LENGTH
CHAR1
CHAR2
CHAR3
CHAR4
TIME OUTBoth transmission
NOTE:Control codes and corresponding character strings are listed in Table 2-8.
Communication
Mode
and reception
HOST → AU
HOST → AU
HOST → AU
HOST → AU
HOST → AU
HOST → AU
HOST → AU
HOST → AU
HOST → AU
HOST → AU
and reception
Contents
of Error
None (normal)Only communication
BCC error or
checksum error
Parity errorAll data printed
Framing errorAll data printed
Overrun errorAll data printed
Frame errorAll data printed
Text length errorAll data printed
Function character
error
Sample data errorAll data printed
Test selecting
information error
Comment errorAll data printed
Transmission or
reception time-out
error
Printout System
(communication
text example)
function No. and
sample data
All data printed
All data printed
All data printed
Only time point,
communication
mode and error
message printed
communication text
(example), an ASCII
control code is
converted into a
character string and
printed, and then
line is fed and
communication text
is printed.
Remarks
(Note)
(4)Notes
(a) Printout is terminated by stop key input. Printout can be stopped after the contents of
trace in one cycle have been printed.
(b) A text is printed on two lines when it is longer than 20 characters.
2 - 75
Page 88
Table 2-6 Control Codes and Corresponding Character Strings Printed
Control Code (NEX)Character StringUse in AU (Analyzer Unit)
SV 12-wayA2-5617Open24 V DCCKDWater supply intake713-0321
SV 22-wayHB-11-X0185Open24 V DCCKDSample syringe713-1059
SV 32-wayHB-11-X0185Open24 V DCCKDReagent syringe713-1059
SV 42-wayHB-11-X0193Open24 V DCCKDIncubation bath water intake 713-1379
SV 52-wayHB-11-X0193Open24 V DCCKD
SV 62-wayHB-11-X0193Open24 V DCCKD
SV 82-wayHB-11-X0193Open24 V DCCKD
SV 92-wayHB-11-X0193Open24 V DCCKD
SV 102-wayHB-11-X0193Open24 V DCCKD
SV 113-wayA2-5619–24 V DCCKD
SV 122-wayNTV-2-HNOpen24 V DC Takasago
SV 132-wayNTV-2-HNOpen24 V DC Takasago
SV 143-wayNRV–24 V DC Takasago Vacuum tank (evacuation)713-0332
SV 152-wayA2-5618Open24 V DCCKD
SV 162-wayMTV-21-SM6MOpen24 V DC Takasago ISE syringe713-0317
SV 172-wayMTV-21-SM6MOpen24 V DC Takasago ISE sample discharge713-0324
SV 182-wayMTV-21-SM6MOpen24 V DC Takasago ISE and REF aspiration713-0325
SD10BA-2A-00TOpen24 V DC Advance ISE aspiration707-0327
3 - 1
Page 94
SV4
SV10
SV9
SV6
SY2
SY3
SY16
SV5
SV8
3 - 2
Page 95
3.3Flow Rate at Each Location
Location
S probe Outside
washing
Inside
washing
R probe Outside
washing
Inside
washing
Stirring and washing
RinsingHigh
concentration
waste solution
probe
Low
concentration
waste solution
probe
Cell blank washing
Nozzle tip washing
Incubation bath flow
rate
Returning from water
supply pump
Flow rate through lamp0.30 or more
Flow Rate
mL/min
270 ± 30
35 ± 5
270 ± 30
120 ± 20
320 ± 30
90 ± 10
90 ± 10
450 ±150
(µL/0.8 sec)
140 ± 15
3.7 ± 0.2
(L/min)
(L/min)
Remarks
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.0/tube L = 650
Tube = Tygon ø3.17 × ø6.35
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.0/tube L =820
Tube = Tygon ø3.17 × ø6.35
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.15/tube L =
1000 Tube = Tygon ø3.17 × ø6.35
SV11/branch tube/tube L = 395/high
concentration waste solution probe
Tube = Tygon ø2.38 × ø3.96
Same as above
SV unit restrictor ø1.5/tube A = 580, specials/tube B = 500
Tube A = Tygon ø3.17 × ø6.35, tube B = Silastic tube ø1.0 ×
ø3.0
SV unit restrictor ø1.5/tube L = 1080
Tube = Tygon ø2.38 × ø3.96
After pouring water into the incubation bath with cells set, flow
velocity on water surface should be measured with cells
removed (in sec/circumference).
Measure with the lamp coolant tube disconnected at the lamp
inlet.
Restrictor ø1.0
3 - 3
Page 96
3.4WASH
3.4.1Details of WASH Processing
START
1)
Operator's
judgment
2)
Made
Abnormal
Unnecessary
Maintenance
screen
WASH necessary?
Necessary
"Execution" input
WASH
"STOP" input
Operation check
Normal
Function:Washing of each mechanism
Necessary :WASH (cell)15 min 20 sec
WASH (ISE)5 min 44 sec
WASH (All)20 min 20 sec
3)
Alarm indication
4)
FD (alarm logging)
END
Step No.Contents of ProcessingReference Document
1)Start from MAINTENANCE screenScreen specifications
2)See next page
3)
4)
When mechanism operates, its operation is checked. If abnormal,
alarm is indicated.
All cells, probes (S and R), stirrer and ISE mechanism are washed.
(b) Specify reaction cell.
(c) Specify ISE.
The ISE mechanism is washed.
NOTE:Specification of ISE for WASH
when without ISE mechanism...............Input cannot be made from the screen.
Specification of all mechanisms
when without ISE mechanism...............Operation is carried out according to "cell"
specification.
3 - 4
Page 97
3 - 5
Page 98
3.5Carry-over Evasion Function
(1)Outline
The carry-over evasion function is provided to prevent occurrence of inaccurate data due
to sample carry-over in photometry assay and ISE tests.
This function works on routine samples alone and does not work on calibrator and control
samples. Carry-over is evaded by changing the measuring sequence among the reagents
or samples, which may cause carry-over, or by execution of washing. The kinds of
evasion/washing are listed below.
(a) Evasion of reagent carry-over due to reagent probe and washing for evasion
(b) Evasion of carry-over between reagents due to cell and washing for evasion
(c) Evasion of sample carry-over due to sample probe and washing for evasion
(a) Evasion of reagent carry-over due to reagent probe and washing for evasion
(I)Condition for carry-over evasion
When the evasion of reagent carry-over due to reagent probe is set, it is
attempted at first by changing the reagent sampling sequence (test sequence).
If this method is unusable, carry-over evasion by washing is executed. That is,
when the sequence of pipetting reagents (R1 to R3) with the reagent probe
matches the specified pattern, carry-over is prevented by (i) to (iii) below. In (ii)
and (iii), throughput is degraded.
(i)For avoiding the relevant pattern, channel registration is changed for a
different test within the test sequence of the same sample.
(ii) When step (i) cannot be taken, a channel is registered after reagent probe
washing cycle.
(iii) Cell is made blank and the system waits till deviation from the specified
pattern.
(II)Method of carry-over evasion
The reagent probe is washed by aspirating detergent (detergent 1 or 2) or
system water (distilled water for washing the inside of probe) and discharging it
into a cell. At this time, the stirring rod is also washed in that cell.
(III)Carry-over evasion-specified pattern
The patterns, for which the evasion of carry-over between reagent types is
specified, are listed below.
"→ ALL" indicates that evasion is required in all types of R1, R2 and R3.
ALL represents 3 reagent types for 3-reagent system, and 2 reagent types for 2reagent system.
(IV)Registration of carry-over evasion-specified pattern
Up to 40 kinds of patterns, for which the evasion of carry-over due to the
reagent probe is specified, can be registered.
3 - 6
Page 99
(V)Probe washing cycle
350
µ
L)
Probe washing employs one (1) machine cycle.
Within one machine cycle, the specified detergent (system water) placed on the
reagent disk is aspirated in the specified volume and discharged into the cell at
the same timing as for reagent aspiration and discharge.
(VI)Measure for ISE
Of the reagents for ISE, the internal standard solution and diluent are applicable
to carry-over evasion. Carry-over evasion cannot be specified for reference
electrode solution.
(VII) Specification from screen
From the screen, the following need be specified; a carry-over giving test and
its reagent type (R1, R2, R3), a carry-over receiving test and its reagent type
(R1, R2, R3), detergent set position on the reagent disk and detergent volume.
An example of specification is shown below.
[GPT] [1][LDH][4][38][350]
↑↑↑↑↑↑
Carryover
giving test
(CH1 to 36)
Reagent
type
(1:R1
2:R2
3:R3)
Carryover
receiving
test
(CH1 to
Reagent
type
(1:R1
2:R2
3:R3)
36, ISE
Detergent set position
(1 to 39...ISE excluded
1 to 36,39...ISE
allowed
W1..........System
water)
ALL)
Detergent
discharge
volume
(50 µL to
(VIII) Examples of evading procedure
Examples of carry-over evading procedure are shown below.
A to C represent tests, and W stands for washing.
Example of Carry-over
Evasion-specified Pattern
A
A
A
R3
R3
R3
B
R2
B
R3
B
R1
A
R3
A
R3
A
R3
Example of Evading Procedure
C
(example of succeeding in evasion by
R2
change in channel registration)
WB
WB
R3
R1
3 - 7
Page 100
(b) Evasion of carry-over between reagents due to cell and washing for evasion
(I)Condition for carry-over evasion
The cell used for analysis of the specified test is not used for analysis in the
next round (after 48 cycles). Instead, it is washed for prevention of carry-over.
(II)Method of carry-over evasion
Cell is washed by aspirating detergent (detergent 1 or 2) or system water and
discharging it into a cell. At this time, washing is facilitated by stirring.
Carry-over evasion of cell is also effected during S. Stop. After completion of
washing all cells, auto stop occurs.
When stop status is set for any cause other than auto stop, cell washing for
evading carry-over due to the previous operation will not be resumed in start
from the standby status.
Execution of cell washing degrades throughput.
(III)Registration of carry-over evasion-specified pattern
Up to 10 kinds of patterns, for which the evasion of carry-over due to cell is
specified, can be registered.
(IV)Specification from screen
From the screen, the following need be specified; a carry-over giving test,
detergent set position on the reagent disk and detergent volume.
An example of specification is shown below.
[TG][38][350]
↑↑↑
Carryover
giving test
(CH1 to 36)
Detergent set position
(1 to 39...ISE excluded
1 to 36,39...ISE allowed
W1…System water)
Detergent discharge
volume
(50 µL to 350 µL)
(V)Cell washing cycle
When detergent discharge volume exceeds 350 µL, detergent is discharged by
using 2 timings among the 3 timings (R1, R2, R3) for reagent aspiration and
discharge in one cycle.
(VI)Example of evading procedure
An example of carry-over evading procedure is shown below.
A to E represent tests, and W stands for washing.
Carry-over Evasion-specified Test
A
a-th cycle............ BAC.......
(a -1)th cycle....... DWE.......
Example of Evading Procedure
3 - 8
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