Hitachi 902 Service manual

Page 1

SERVICE MANUAL

FOR

MODEL 902 AUTOMATIC

ANALYZER
Copyright Hitachi, Ltd. 1997. All rights reserved. Printed in Japan. Part No. 713-9039 KN-K (H-LT)
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MODEL 902 AUTOMATIC ANALYZER
CONTENTS
10. CIRCUIT DIAGRAMS............................................................................................... 10-1
11. CROSS WIRING REFERENCE ............................................................................... 11-1
12. INSTALLATION........................................................................................................ 12-1
13. MAINTENANCE AND INSPECTION ........................................................................ 13-1
14. ADJUSTMENT SPECIFICATIONS........................................................................... 14-1
15. OEM......................................................................................................................... 15-1
16. SYSTEM INTERFACE.............................................................................................. 16-1
- i -
Page 3

1.PRODUCT SPECIFICATIONS

1.1 Overall System.........................................................................................................1-1
1.2 Sampling System.....................................................................................................1-2
1.3 Reagent System.......................................................................................................1-3
1.4 Reaction System......................................................................................................1-4
1.5 Photometic System..................................................................................................1-5
1.6 ISE (option)..............................................................................................................1-5
1.7 Installation Conditions..............................................................................................1-6
1.8 User Interface...........................................................................................................1-6
1.9 Dionized Water........................................................................................................1-6
1.10 Analytical Methods...................................................................................................1-7
1.11 Accuracy Methods....................................................................................................1-7
1.12 Data Storage............................................................................................................1-7
1.13 System Interface......................................................................................................1-8
1.14Options for Analyzer.................................................................................................1-8
1 - 0
Page 4
1.PRODUCT SPECIFICATIONS

1.1 Overall System

Configuration : Console type automatic analyzer, with analytical and operation units
integrated
Principle : Discrete type, random access, single-line multi-analysis system;
entire reaction monitoring system
Analytical methods : Colorimetry (absorbance measurement), ion selective electrode
method (ISE; option)
Throughput : Colorimetry ; 200 tests/hr (Photometry only max. 300 tests/hr
inclusive of ISE)
No. of analysis items: Colorimetry ; 36
ISE ; 3 (Na, K, Cl) Serum indexes ; 3 Calculation items; 8
Reaction time : 10 minutes max.; 3, 4, 5, 10 minutes for concentration calculation
(same as Model 7070/7170)
Measurable samples : Serum (or blood plasma) and urine; one sample kind per channel
Application : Emergency (stat) and routine analyses
Test item selection : Via entry from operation panel, entry from system interface, entry
from no. of items settable (11 kinds)
1 - 1
Page 5

1.2 Sampling System

Sample container : Hitachi standard sample cup
Sample tube ;diameter 13 to 17 mm
length 75 to 100 mm
Sample disk : 60 positions
Outer row;35 positions (routine and stat samples)
(with free adapter, barcode reader attachable)
Inner row;25 positions (standard + control samples (22),
rinse solutions (3))
Detergents : 3 kinds
(W1;for sample probe rinsing) HITERGENT (W2;for sample carryover evasion) ISE RINSE SOLUTION (W3;for sample carryover evasion) HICARRYNON
Sampling mechanism : Pipetter driven by stepping motor
2 to 50 µL of sample/test (in 0.1 µL steps)
Pipetting mode : Normal ; prescribed volume discharged at cell
bottom
Aqueous sample; system water solution for sample probe
internal rinsing usable as STD-1
Sample pre-dilution not performed
Insufficient sample detection: Resistance detection method
Sample ID : Barcode reader (option)
Following code shemes are usable in combination; CODE 39, CODE 128, INTERLEAVED 2 OF 5, NW7 (Modulas 10, Modulas 16)
Automatic rerun : Auto rerun function not provided
Stat sample analysis : Routine sample analysis interruptible for stat sample analysis
Sample carryover : Sample carryover evasion function available; 2 kinds of
detergents settable on sample disk inner row
Sample conveyance : Can be sampled directly from belt-line
1 - 2
Page 6

1.3 Reagent System

Reagent disk : One disk, 40 positions
(20 positions each on inner and outer rows)
Reagent cooling : Cooling water circulating system (3 to 15°C) (option for China-
destined instrument)
Reagent bottle : 50 mL, 20 mL (adapter necessary)
(without barcode, concentrated reagent unusable)
Detergents : HITERGENT (50 mL); position 40 (fixed)
(for addition to reaction bath) Detergent 1; for reagent probe, stirring rod rinsing, reagent
probe carryover evasion, reaction cell carryover evasion
Detergent 2; for reagent probe carryover evasion, reaction cell
carryover evasion
(settable on reagent disk inner row)
ISE reagent : Internal standard solution(position 37 (fixed))
Diluent (position 38 (fixed))
Pipetting mechanism : Pipetter driven by stepping motor; 20 to 350 µL/test (in 1 µL
steps)
Reagent addition timing: 3 steps (0, 1.5 and 5 minutes); throughput is maintained even
when adding 3 reagents.
Carryover evasion : Rinsing for carryover evasion possible; throughput may decrease
by specifying carryover evasion.
1 - 3
Page 7

1.4 Reaction System

Reaction disk : Turntable type disk (10-minute reaction per rotation)
Reaction cell : Optical path length 6 mm (area 6 × 5 mm)
No. of reaction cells : 48
Reaction cell control : One rotation + one pitch feed (18 seconds)
Sample pipetting position: One position (fixed)
Reagent pipetting position: One position (fixed)
Reaction temperature : 37 ± 0.1°C (warm water circulating system)
Stirring : By means of stirring rod rotation
Timing for stirring; after each addition of R1,R2,R3 Stirring possible for a minimum volume of 100 µL
Stirring position : One position (fixed)
Reacting solution volume: Minimum; 250 µL (minimum required volume for photometry)
Maximum; 500 µL (temperature control, rinsing upper limit)
Photometric position : One position (fixed)
Cell rinsing : After completion of photometry (rinsing 3 times; rinsing with
detergent not performed)
Carryover evasion : Rinsing for carryover evasion possible; throughput may decrease
by specifying cell carryover evasion
1 -4
Page 8

1.5 Photometric System

Photometer : Multiwavelength photometer (absorptiometry)
Wavelengths : 12 wavelengths
(340, 376, 415, 450, 480, 505, 546, 570, 600, 660, 700, 800 nm)
Monochromator : Grating
Detector : Photodiode
Linearity : Up to 2.5 Abs (10 mm cell conversion)
Photometric method: Direct photometry of reaction cell (at one or two wavelengths)
Correction : Cell blank correction prior to analysis
(passed cell blank measurement alone)

1.6 ISE (option)

Electrodes : Flow cell type, liquid-membrane ISE cartridge
Reference electrode flow path: 1 MKCL, liquid flow path
Measuring temperature : 36°C ± 2°C (warm water circulating system)
System : Indirect (dilution) potentiometry, 50-times dilution
Measuring cycle : 36 sec/sample (18 sec for sample, 18 sec for internal
standard solution)
Measured items : Na, K, Cl
Measurable samples : Serum and urine
Linearity : Na;10 to 250 mmol/L
K ;1 to 100 mmol/L Cl;10 to 250 mmol/L
Reagent bottle : Internal standard solution ; 50 mL max.
Diluent ; 50 mL max. Reference electrode solution; 500 mL max.
1 - 5
Page 9

1.7 Installation Conditions

Power requirement : 230 V, 50/60 Hz, less than 1.5 kVA
Deionized water consumption: Less than 15 L/hr
Waste liquid drain : 2 systems (for concentrated and diluted liquids)
Ambient temperature/humidity: Temperature; 18 to 30°C
Humidity ; 20 to 80% (non-condensing)
Analyzer dimensions : 720 W × 720 D × 1085 H mm
Analyzer weight : Within 200 kg
BTU : 1300 kcal/hr max.
Noise (mean) in operation : < 55 dB

1.8 User Interface

Application : Routine analysis for Asia version
Display : Backlighted LCD; 256 × 128 dots, graphic
Keyboard : Touch screen keys (72 keys)
Printer : Thermal roll-paper printer (20 digits)
Multi-language compatibility : Display; Japanese/English/Chinese/German/Spanish applicable
Printer; English alone

1.9 Deionised Water

Pressure : 0.5 to 3.5 kgf/cm
2
Conductivity: 1 µs/cm or less, germ-free
1 - 6
Page 10

1.10Analytical Methods

Assay modes : One-point
One-point end (+ prozone check) Two-point rate Two-point end (+ prozone check) Three-point two-item One-point rate two-item Rate A (+ sample blank correction) Rate A (+ serum indexes) Rate B two-item (same wavelength) Rate B two-item (different wavelengths) ISE
Data alarms : Based on Model 7070/7170
Standard solution : 22 kinds max. (positions to be shared with control sera)
Calibration types : Linear (2-point linear)
K factor 4 parameter LOGIT-LOG 5 parameter LOGIT-LOG Spline Segmented line
Calibration method : At startup only; all points (FULL) and reagent-blank-corrected
calibration
Calculation channels : For 8 channels
Test-to-test compensation : For 8 channels

1.11Accuracy Control

Control serum : 5 kinds max. (positions shared with standard solutions)

1.12Data Storage

Routine sample data : 400 samples (in data disk)
Stat sample data : 50 samples (in data disk)
Control sample data : 5 kinds × 30 (in SRAM)
1 - 7
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1.13System Interface

Interface : RS-232C and current loop
Communication protocol: Based on Model 7070/7170
Communication details : Communication with host; communication details based on Model
7070/7170

1.14Options for Analyzer

ISE
Sample ID accessory : Model ; BL180
Maker ; Keyence Barcode spec.; CODE 39, ITF, NW7 (Modulas 10, Modulas 16),
CODE 128
1 - 8
Page 12

2. TROUBLESHOOTING

2.1 Alarm Code Table ....................................................................................................2-1
2.1.1 LCD Display Alarm .....................................................................................2-24
2.2 Motor Control Alarms................................................................................................2-26
2.2.1 Operation Check Procedure at Occurrence of Alarm .................................2-27
2.3 Parameter Check .....................................................................................................2-29
2.3.1 Processing Flow .........................................................................................2-29
2.3.2 Details of Parameter Check........................................................................2-30
2.3.3 Details of Twin Test Simultaneous Analysis ...............................................2-34
2.4 Data Alarm...............................................................................................................2-35
2.4.1 Data Alarm Registratin Flow.......................................................................2-35
2.4.2 Data Alarm Code List..................................................................................2-36
2.4.3 Data Alarm Codes ......................................................................................2-37
2.4.4 ISE Data Alarms.........................................................................................2-49
2.4.5 Alarm Check Method..................................................................................2-52
2.4.6 Check and Set Alarm of Each Data............................................................2-60
2.4.7 Details of Data and Alarm Outputs Resulting from Calibration...................2-61
2.4.8 Output Check List for Each Photometry Assay CALIB. METHOD .............2-62
2.5 Retry Code Table.....................................................................................................2-64
2.5.1 Logging Program List......................................................................................2-65
2.6 Daily Alarm Trace.....................................................................................................2-67
2.6.1 Cumulative Alarm Trace.............................................................................2-69
2.6.2 Parameter Code List...................................................................................2-72
2.6.3 Communication Trace.................................................................................2-74
2.6.4 Cumulative Instrument Operation List.........................................................2-78
2.6.5 FD File Management..................................................................................2-79
2 - 0
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2. TROUBLESHOOTING

2.1 Alarm Code Table

Category Alarm Name Category Alarm Name
1 STIRRER 38 VAC. TANK 2 39 3 RINSE 40 4 41 LAMP 5 R.DISK 42 6 S.PROBE 43 7 44 8 S.DISK 45 CELL BNK1
9 46 CELL BNK2 10 47 ADC1 ? 11 S. SHORT 48 ADC2 ? 12 49 ADC3 ? 13 50 BARCODE 1 14 51 15 S. SYRINGE 52 16 REAG. PROBE 53 17 54 BARCODE 2 18 REAG. DISK 55 19 56 20 57 REAG. SHORT 21 58 22 R. SYRINGE 59 REAG. LEVEL 23 60 24 ISE SIPPER 61 25 62 26 ISE SYRING. 63 27 ISE STOP ? 64 28 TEMP CONT. 65 29 INC. WATER 66 30 REF. WATER 67 TS OVER 31 DIST. WATER 68 PATNT OVER 32 DIST. SENS. 69 SAMP. END 33 70 34 RESERVOIR 71 DC POWER 35 72 FUSE 36 73 POWER FAIL 37 SIPPER 74
2 - 1
(contd)
Page 14
Category Alarm Name Category Alarm Name
75 MOTOR CONT. 114 ACI ERROR 76 MOTOR TOUT 115 77 116 78 117 79 118 80 119 FD WRITE ? 81 120 FD READ ? 82 121 NO FD 83 STANDARD ? 122 84 CALIB. 123 FD PROTECT 85 CALIB. SD ? 124 86 SENS. ? 125 PRINTER 87 126 SYSTEM I/F 88 127 89 128 90 ISE LEVEL 129 91 ISE NOISE 130 WATER EXG. 92 ISE PREP. 131 93 ISE SLOPE 132 94 ISE I. STD 133 95 REF. SHORT 134 96 TWIN TEST ? 135 97 136 98 CHEM. PARAM ? 137 99 CLB. PARAM ? 138
100 VOLUME ? 139 101 140 PANEL I/F 102 141 REAGNT ? 103 CMP. TEST ? 142 104 S. INDEXES ? 143 105 144 106 ON BOARD ? 145 CELL C. O. 107 REAG. POS ? 146 ISE C. O. 108 CLB (IS) POS 147 109 148 110 149 111 150 112 151 113 152
2 - 2
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Alarm Control No.
1 to 8 STIRRER 1 1 STOP In ascending action of the
21,22 RINSE 3 1 STOP The rinsing mechanism does
Alarm Category
1 2 STOP In ascending action of the
1 3 STOP In descending action of the
1 4 STOP When the stirrer moves
1 5 STOP The stirrer does not come to 1 6 STOP At resetting, the stirrer does
1 7 STOP At resetting, the stirrer does
1 8 STOP In rotation of the stirrer, it is
3 2 STOP The rinsing mechanism does
Sub­code
Level Description Remedy
stirrer, it does not reach the upper dead point (on the rinsing bath side).
(Alarm at the first upper dead point after resetting will be issued from other than the cell side.)
stirrer, it does not reach the upper dead point (on the cell side).
stirrer, it does not leave the upper dead point.
toward the rinsing bath, it does not reach the rinsing bath position.
the cell position. not reach the rinsing bath
position (home position) in its return movement to the home position.
not leave the rinsing bath position (home position) in its departing movement from the home position.
not set at the upper dead point.
not reach the upper dead point in ascending motion.
not leave the upper dead point in descending motion.
Check the upper dead point detector.
Same as above
Same as above
Check the home detector.
Check the detector on the cell side.
Check the home detector.
Same as above
Check the upper dead point detector.
Check the upper dead point detector.
Same as above
2 - 3
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Alarm Control No.
Alarm Category
Sub­code
Level Description Remedy
61 to 65 R. DISK 5 1 STOP The reaction disk cannot
recognize its stop position.
5 2 STOP The reaction disk does not
stop at the specified position.
5 3 STOP At resetting, the reaction
disk cannot recognize its home position.
5 4 STOP At resetting, the first cell
on reaction disk does not stop at the specified position.
5 5 STOP When the reaction disk
turns, the serum probe, reagent probe, stirrer or rinsing mechanism is not set at the upper dead point
(on the cell side). (When this alarm is
issued, another alarm (on rinsing or stirring mechanism) may concur.)
71 SAMPLE
PROBE
6 1 S.STOP/
STOP
(Note 1)
The serum probe does not reach the upper dead point in ascending motion (on other than the cell side).
(Alarm at the first upper dead point after resetting will be issued from other than the cell side.)
72 to 85 SAMPLE
PROBE
6 2 STOP The serum probe does not
reach the upper dead point in ascending motion (on the cell side).
6 3 S.STOP/
STOP
(Note 1)
The serum probe moves down abnormally in descending action (on other than the cell side).
(±3 mm from cup bottom, 0 to 4 mm from cell
bottom)
6 4 STOP The serum probe moves
down abnormally in descending action (on the cell side).
6 5 S.STOP/
STOP
(Note 1)
The serum probe does not go down from the upper dead point in descending motion.
NOTE: 1. S.STOP may be issued only during operation.
(cont’d)
Check the detectors for stop positions on the inner and outer tracks.
Same as above
Check the home detector.
Check the home detector or inner/outer track detector.
(1) Perform resetting. (2) Check the upper
dead point detector of the mechanism which caused alarm.
Check the upper dead point detector.
Same as above
(1) Check the liquid
level detector.
(2) Replace the liquid
level detector PC board.
Same as above
Check the upper dead point detector.
2 - 4
Page 17
Alarm Control
Alarm Category
No.
72 to 85 SAMPLE
6 6 STOP The serum probe does not
Sub-
code
Level Description Remedy
PROBE
6 7 S.STOP/
STOP
(Note 1)
6 8 S.STOP/
STOP
(Note 1)
6 9 S.STOP/
STOP
(Note 2)
6 11 S.STOP/
STOP
(Note 1)
6 12 WARNINGThe serum probe moves
6 13 S.STOP/
STOP (Note 2)
6 14 STOP In rotation of the serum
6 15 STOP In rotation of the serum
101 to 106
SAMPLE DISK
8 1 S.STOP/
STOP (Note 2)
8 2 S.STOP/
STOP (Note 2)
8 3 S.STOP/
STOP (Note 2)
8 4 S.STOP/
STOP (Note 2)
NOTE: 2. S.STOP may be issued only during operation.
go down from the upper dead point in descending motion (on the cell side).
Detection of abnormal descending motion of the serum probe remains on.
When serum probe turns to the cell side, the cell position cannot be detected.
When the serum probe turns from the cell side to other position, it does not come off the cell position.
Before the probe goes down, the liquid level detector is already turned on.
down abnormally in descending action (only at turning of the serum probe in adjustment).
In rotation of the serum probe, it is not set at the upper dead point.
probe from the reset position, it does not come off the reset position.
probe to the reset position, it cannot detect the reset position.
The sample disk cannot detect the stop position on outer track.
The sample disk does not stop at the specified position on outer track.
The sample disk cannot detect the stop position on inner track.
The sample disk does not stop at the specified position on inner track.
(cont’d)
Check the upper dead point detector.
Refer to alarm code 6-3.
Check the home detector.
Same as above
Refer to alarm code 6-3.
Same as above
Check the upper dead point detector.
Check the home detector.
Same as above
Check the outer track detector.
Same as above
Check the inner track detector.
Same as above
2 - 5
Page 18
(cont’d)
Alarm Control
Alarm Category
No.
101 to 106
SAMPLE DISK
107 SAMPLE
DISK
251 to 310
551 to 552
561 to 568
SAMPLE SHORT
SAMPLE SYRINGE
REAGENT PROBE
Sub­code
Level Description Remedy
8 5 STOP At resetting, the sample disk
cannot detect the home position.
8 6 STOP At resetting, the sample disk
does not stop at the specified point of home position.
8 7 STOP At resetting, the sample disk
does not leave the home position.
11 1 to 60WARNING In sipping from the sample
cup, sample on the sample disk is inadequate.
Sub-code groups
Routine/ stat samples:1 to 35
Control STD:36 to 57 W1 to W3 :58 to 60
15 1 S.STOP/
STOP
15 2 S.STOP/
STOP
The serum syringe does not reach the upper dead point.
The serum syringe does not go down from the upper dead point.
16 1 STOP The reagent probe does not
reach the upper dead point in ascending motion.
16 2 STOP The reagent probe moves
down abnormally in descending motion.
16 3 STOP The reagent probe does not
go down from the upper dead point in descending motion.
16 4 WARNINGDetection of abnormal
descending motion of the reagent probe remains on.
Check the home detector.
Same as above
Check the home detector.
Add sample.
Check the upper dead point detector.
Same as above
Check the upper dead point detector.
Check liquid level detection.
Check the upper dead point detector.
Check the descending error detector.
2 - 6
Page 19
Alarm Control No.
561 to 568
Alarm Category
REAGENT PROBE
16 5 STOP When the reagent probe
Sub­code
Level Description Remedy
turns toward the cell, it cannot detect the cell position.
16 6 STOP When the reagent probe
turns from the cell side to other position, it does not leave the cell position.
16 7 STOP Before the probe goes down,
the liquid level detector is already turned on.
16 8 STOP In rotation of the reagent
probe, it is not set at the upper dead point.
581 to 593
REAGENT DISK
18 1 STOP The stop position of reagent
disk cannot be detected.
18 2 STOP The reagent disk does not
stop at the specified position.
18 3 STOP The home position of reagent
disk cannot be detected.
621 to 622
REAGENT SYRINGE
22 1 STOP The reagent syringe does not
reach the upper dead point.
22 2 STOP The reagent syringe does not
move down from the upper dead point.
641 ISE
SIPPER
24 1 STOP The sipper nozzle does not
reach the upper dead point (during resetting/ operation).
642 ISE
SIPPER
661 to 662
ISE SYRNG
671 ISE STOP
OK ?
24 2 WARNING/
STOP
(Note 4)
26 1 WARNING/
STOP
(Note 4)
26 2 WARNING/
STOP
(Note 4)
27 1 WARNING/
STOP
(Note 4)
The sipper nozzle does not leave the upper dead point.
The sipper syringe does not reach the upper dead point.
The sipper syringe does not leave the upper dead point.
The ISE function is stopped due to alarm. (This warning is indicated when restart in the sampling stop status was attempted.)
NOTES: 3. S.STOP may be issued only during operation.
4. Though the photometry assay function works, the ISE function does not work.
(cont’d)
Check the home detector.
Same as above
Check liquid level detection.
Check the upper dead point detector.
Check home detection or count detector.
Same as above Check home
detection. Check the upper
dead point detector. Same as above
Check the upper dead point detector of ISE sipper.
Same as above
Check the upper dead point detector of ISE Syringe.
Check alarm log and deal with the alarm.
2 - 7
Page 20
(cont’d)
Alarm Control
Alarm Category
No.
681 TEMP
CONTROL
28 1 WARNING The water temperature of
Sub­code
Level Description Remedy
incubation bath is higher than 45.0°C.
28 2 WARNING The water temperature of
incubation bath is outside a range of 37 ± 0.5°C.
(This is checked only at operation.)
691 INCUBA-
TOR
29 1 WARNING The water level of
incubation bath is too low.
WATER
701 REF
WATER
30 1 WARNING A period of 24 hours has
passed since exchange of incubation bath water.
711 DISTILLED
WATER
31 1 STOP The water level of distilled
water tank is too low. (This alarm will not be
issued during initialization and water exchange.)
712 DIST
WATER
721 DIST
WATER SENSOR
724 RESER-
VOIR
31 2 WARNING The water level of distilled
water tank is too low.
32 1 WARNING The water level sensor in
distilled water tank is abnormal.
34 1 WARNING The waste solution reservoir
is full.
771 SIPPER 37 1 STOP The negative pressure of
vacuum pump is too low.
781 VACUUM
TANK
811 to 813
PHOTOME­TER LAMP
38 1 WARNING Water is accumulated in the
vacuum tank.
41 1 WARNING In passed cell blank
measurement, a value of more than 3.3 Abs is indicated in any one of 4 measurements.
(3 times or less)
41 2 S.STOP
(Restart un­allowable
41 3 S.STOP
(Restart un-
In passed cell blank measurement, a value of more than 3.3 Abs is indicated for all of ADC1,
)
ADC2 and λ
to λ12.
1
The above alarm code 41-1 is issued 10 times consecutively.
allowable)
Check the thermistor or thermostat of heater.
Same as above
Replenish water or check the drain solenoid valve.
Exchange incubation bath water.
Check the water level sensor.
Same as above Check float switch.
Check waste solution tank detection.
Check vacuum level at vacuum suction.
Check SV12 or SV14. (1)Replace light
source lamp.
(2)Check 12 V
power supply.
2 - 8
Page 21
(cont’d)
Alarm Control
Alarm Category
No.
1231 CELL
BLANK
1241 to 1400
1441 to
PASS CELL BLANK
ADC1? 47 1 WARNING Any one of I/O error,
1444
Sub­code
45 1 S.STOP
Level Description Remedy
In passed cell blank (Restart un­allowable)
measurement, any one of
ADC1, ADC2, λx and λy
differs from the reference
value (Note 5) by more than
0.1 Abs through 10
consecutive cycles.
(Counting is not made in
use for stopped cell blank
test.)
46 1 to 48WARNING In 4 passed cell blank
measurements, any one of
ADC1, ADC2, λx and λy
differs from the reference
value (Note 5) by more than
0.1 Abs twice or more.
(In case any one of those
values differs only once, the
average value of normal
data is treated as a value of
passed cell blank.)
boundary error of parameter
block pointer, I/O device
busy, channel error and I/O
device error (PC board not
mounted, device
inoperable, time-out) has
occurred.
47 2 WARNING A/D count value remains
at 0.
After A/D conversion, interruption is impossible.
The command or parameter given to A/D PC board is abnormal.
A/D conversion cannot be completed.
(Time-out occurs.) (Reference voltage is
checked.)
47 3 WARNING The number of A/D starts
cannot be reset to 0.
(This is checked at reaction
measurement.)
(1)Carry out cell
washing.
(2)Replace the cell.
Same as above
Replace ECPU230 or EMIO100 PC board.
(1)Replace Log Amp
PC board.
(2)Check 2 V and
6 V reference voltages.
Same as above
2 - 9
Page 22
(cont’d)
Alarm Control No.
1441 to 1444
1451 to 1453
1461 to 1462
1471 to 4506
Alarm Category
ADC1? 47 4 WARNING A/D count for 2 V is
Sub­code
Level Description Remedy
Same as above
abnormal.
A/D count for 6 V is
abnormal. Normal count for 2 V
(7547 < count value <
8341) Normal count for 6 V (22460 < count value <
25023) (This is checked at measurement of reference voltage.)
ADC2? 48 1 WARNING Refer to alarm code 47-1. Replace ISE AMP PC
board.
48 2 WARNING Refer to alarm code 47-2. Same as above 48 3 WARNING Refer to alarm code 47-4.
Reference voltage for
ISE: 2 V and 8 V
Normal count for 2 V
(7547 < count value <
8341)
Normal count for 8 V
(30184 < count value <
33364)
Normal offset value
(22811 < count value <
40547)
ADC3? 49 1 WARNING Refer to alarm code 47-2. Replace EMIO100
49 2 WARNING A/D count for 2 V is
abnormal.
Same as above
PC board. Same as above
A/D count for 6 V is
abnormal.
Normal count for 2 V
(12452 < count value <
13763)
Normal count for 6 V
(37356 < count value <
41288)
BARCODE1 50 1 to 35WARNING Data reception from the
barcode reader has not been completed before ID reception time-out.
(Sub-code indicates the
position No. on disk.)
Replace the label or barcode reader.
2 - 10
Page 23
(cont’d)
Alarm Control
Alarm Category
No.
1801 to
BARCODE2 54 1 to 5WARNING An error has occurred in
1805
1931 to 1971
2231 to 2271
2781 to 2830
2851,
REAGENT SHORT
REAGENT LEVEL
UN­ASSIGNED
TS OVER 67 1 WARNING Because 400 samples are
2852
2861 PATIENT
SAMPLE OVER
Sub­code
Level Description Remedy
communication with the barcode reader. (Parity error, framing error or overrun error)
Sub-code indication
1:Unassigned 2:Unassigned 3:Sample disk barcode
reader 4:Unassigned 5:Unassigned
57 1 to 40WARNING The volume of reagent to
be sipped from a reagent bottle is inadequate.
The reagent volume is a total amount of one kind of reagent for each test item.
(1)Sub-code indicates
position No.
(2)Positions 37 and 38
are used for ISE.
(3)Position 40 is used for
HITERGENT.
(4)Position 39 is used for
HIALKALI.
59 1 to 40WARNING Reagent volume is smaller
than the remaining reagent check value specified with system parameter.
registered in routine sample test selecting information, new test selecting information cannot be registered from the host.
68 1 WARNING Because 400 samples are
registered in routine sample test selecting information, a new routine sample cannot be analyzed.
Check the communication cable of barcode reader.
Set new reagent.
Set reagent newly.
After completion of analysis, register TS again.
Same as above
2 - 11
Page 24
(cont’d)
Alarm Control No.
2891 to 2894
Alarm Category
DC POWER 71 1 STOP 15 V DC power supply is
Sub­code
Level Description Remedy
Replace the ±15 V
abnormal.
power supply module.
71 2 STOP -15 V DC power supply is
Same as above
abnormal.
71 3 WARNING 12 V lamp power supply is
abnormal. 71 4 STOP 5 V power supply is abnormal. 71 11 E.STOP 24 V DC
Replace the 12 V power supply.
5 V for other than CPU board
2911 FUSE 72 1 E.STOP AC fuse has blown. Replace the F3
fuse.
2921 POWER
FAIL
2941 to 2973
MOTOR CONTROL-
73 1 WARNING Power supply to the
instrument is interrupted 75 1 to 18 STOP Data cannot be written into
the motor controller.
Check power supply.
Replace ECPU230.
LER
2991 to 3023
MOTOR TIMEOUT
76 1 to 18 E.STOP Motor operation was
abnormal. Error has been
detected in the time-out check
of motor controller.
(1) Replace
ECPU230.
(2) Check the
mechanism which caused
Sub-code Motor
alarm.
1 Reaction disk
2 Sample disk 3 Reagent disk 4 Rinsing mechanism
up/down
5 Sample arm
up/down
6 Sample arm
rotation
7 Reagent arm
up/down
8 Reagent arm
rotation 9 Stirrer up/down 10 Stirrer rotation 11 ISE sipper up/down 12 (Unassigned) 13 Serum syringe 14 Reagent syringe 15 ISE syringe 16 (Unassigned) 17 GMCNT 18 (Unassigned)
2 - 12
Page 25
(cont’d)
Alarm Control No.
3101 to 3189
3251 to 3291
3401 to 3438
Alarm Category
Sub­code
STANDARD? 83 1 to 40
(ch.)
CALIBRA­TION
84 1 to 40
(ch.)
CALIB.SD? 85 1 to 36
(ch.)
Level Description Remedy
WARNING <<Photometry assay>>
(1) In calibration, the
STD absorbance data is indicated with alarm.
(2) In calibration, data
calculation is disabled.
(Photometry assay)
Replace STD sample.
Check the concentration parameter.
(ISE assay)
Same as above
<<ISE assay>>
(1) In calibration, the
potential data of standard or internal standard solution is indicated with ADC error, insufficient sample alarm, noise alarm or level alarm.
(2) In calibration, data
calculation is disabled.
(The result of calibration is not updated nor saved onto FD.)
WARNING <<Photometry assay>>
The factor value 'K'
determined in calibration
(Photometry assay) (ISE assay)
Same as above differs from the previous value by more than ±20%.
<<ISE assay>>
The calibrator concentration value and slope value determined in calibration differ from the previous values by more than the compensation limit (%).
WARNING The mean error determined
in multi-point calibration is
Replace STD and
check again. larger than the SD limit (input value).
2 - 13
Page 26
(cont’d)
Alarm Control No.
3551 to 3588
Alarm Category
SENSITIV­ITY?
Sub­code
86 1 to 36
(ch.)
Level Description Remedy
WARNING In linear (with 2 to 6 points) or
nonlinear calibration, a difference between the mean STD (1) absorbance and the mean STD (N)
(Note 7 )
The result of calibration is not updated nor saved onto FD.
absorbance is smaller than the sensitivity limit (input value).
NOTES :
7.N:=2 for linear (2points)
=2 to 6 for nonlinear
and linear (3 to 6 points)
(Span point input value)
8.If either STD (1) or STD
(N) alone has been measured, the absorbance value of the other STD is checked using the previous data.
4151 to 4153
4161 to 4163
ISE LEVEL 90 1 WARNING The mean potential value
(EAV) at three out of five measurement points of internal standard solution is outside the following range.
(Internal standard)
Na:-90.0mV EAV -10mV
OK
90 2 WARNING K:-90.0mV EAV -10mV
OK
90 3 WARNING Cl:100.0mV EAV
180.0mV
OK
ISE NOISE 91 1 WARNING A difference (FIV) between
maximum and minimum potential values at three of five measurement points of internal standard solution is outside the following range.
(Internal standard, sample)
Na:0.7mV < FIV(2) - FIV(4)
91 2 WARNING K :1.0mV < FIV(2) - FIV(4) Same as above
(1)Replace STD
and carry out calibration.
(2)Replace the Na
electrode.
(1)Same as above (2)Replace the K
electrode. (1)Same as above (2)Replace the Cl
electrode. Carry out reagent
priming and check for bubble formation.
91 3 WARNING Cl:0.8mV < FIV(2) - FIV(4) Same as above
2 - 14
Page 27
Alarm Control No.
4171 to 4173
Alarm Category
Sub­code
Level Description Remedy
ISE PREP. 92 1 WARNING Upon calibration, the slope
value is within the following range.
Na : 45.0mV ≤ Slope value
49.9mV or
68.1mV ≤ Slope value
92 2 WARNING K : 45.0mV ≤ Slope value
49.9mV or
68.1mV ≤ Slope value
92 3 WARNING Cl : -39.9mV ≤ Slope value
-35.0mV or
-68.1mV Slope value
(cont’d)
Make sure that the standard solution and reagent are set properly.
Make sure that the standard solution is free from concentration or deterioration.
Make sure that the electrodes (Na, K, Cl) are within their guaranteed life.
4181 to 4183
4191 to 4193
ISE SLOPE 93 1 WARNING (1) In the result of
calibration, the slope value is within the following range.
(2) The response
characteristic of electrode is poor (in case carry-over rate (A) is as indicated below).
Na : (1)SLOPE < 45.0mV
(2)0.232 < A
93 2 WARNING K : (1)SLOPE < 45.0mV
(2)0.160 < A
93 3 WARNING Cl : (1)SLOPE < -35.0mV
(2)0.490 < A
ISE I.STD 94 1 WARNING The concentration of internal
standard solution (C(IS)) is within the following range. Na : C(IS) < 120.0mEq/L or
160.0mEq/L < C(IS)
94 2 WARNING K : C(IS) < 3.0mEq/L or
7.0mEq/L < C(IS)
94 3 WARNING Cl : C(IS) < 80.0mEq/L or
120.0mEq/L < C(IS)
Refer to alarm codes 92-1 to 3.
Same as above
Same as above
(1) Replace STD
and carry out calibration again.
(2) Replace t he
internal standard solution.
Same as above
Same as above
2 - 15
Page 28
Alarm Control
Alarm Category
No.
4201 to 4203
ISE REAGENT SHORT
4211 TWLN
TEST?
Sub­code
Level Description Remedy
95 1 Unassigned
(Liquid level detection for IS/DIL solution)
95 2 Unassigned (Same as
above)
95 3 WARNING The volume of reference
electrode solution is 30 mL or less.
96 1 to 36
(ch.)
WARNING (1) when analyzing two
tests at a time, the assigned method for the corresponding test is inadequate.
(2) Assignment of the
corresponding test is being done or not being done when analysis for two tests is not being made at the same time.
(3) When analyzing two
tests at the same time, the analytical parameters are not identical. Sub-codes (1 to 36) signify the channel numbers.
(cont’d)
Replace the reference electrode solution with new one. Check the ISE reagent volume.
Start setting in order from the tests that are designated by the Measured Point of the first half of the 1 channel 2 Test Analysis Method (3 Point, 1 Point and Rate, Rate B)
When not analyzing two tests at the same time, do not designate 'Two Test Analysis' for the Analysis Parameter.
With the Analysis Parameter screen for the applicable test, unify all the parameters below for the Simultaneous 2 Test Analysis Test.
Analytical Method
Reaction Time
Sample Volume
Reagent
Pipetting volume (R1-R3)
Calibration
Method
Calibration Point
Standard solution
volume
Standard solution
position
2 - 16
Page 29
Alarm Control No.
4511 to 4548
4661 to 4698
Alarm Category
CHEMISTRY PARAME­TER?
CALIBRA­TION
PARAME­TER CALIB.
Sub-
code
98 1 to 36
(ch.)
99 1 to 36
(ch.)
Level Description Remedy
WARNING(1)The relationship
between assay code and
Correct the
parameter. photometric point is improper.
(2)The assigned
photometric point lags behind the specified reaction time. (Operation is impossible.)
WARNING (1)The relationship
between assay code and
Check parameter
and input it again. calibration type is improper.
(2)Necessary calibration
points for calibration type are not input.
(3)Necessary standard
positions for calibration are not input.
(4)The relationship
between calibration type and calibration method is improper.
(5)The standard
concentration values are not set in ascending order. (Except for STD (3) and (4) for isozyme)
(6)Concentration value is
not zero when '99' is entered for POS. of STD (1).
1.The photometry assay data in other than manual mode is checked.
2.Operation is unallowable.
3.Check in (4) is made with regard to specifications of time­out calibration, lot-to-lot calibration, bottle-to­bottle calibration and test selecting information.
(cont’d)
2 - 17
Page 30
(cont’d)
Alarm Control No.
4811 to 4848
Alarm Category
VOLUME CHECK?
Sub-
code
100 1 to 36
(ch.)
Level Description Remedy
WARNING (1)The total reagent
volume up to the last photometric point is more than 250 to 500 µL.
(2)The volumes of
reagents 1 to 3 are all
Check parameter. If the improper condition indicated by alarm can be detected, correction and reentry are required.
zero.
(3)The reagent volume
having a timing behind the reaction time is not zero.
(4)The total liquid volume
of sample and reagent is less than 250 µL.
1.When '999' (stirring only) is specified for reagent volume, 0 µL is taken for the volume.
However, when '999' is specified for the reagent volume having a timing behind the reaction time, an error occurs.
2.Operation is unallowable.
3.The final liquid volume is a total volume of sample and reagent within 250 to 500 µL.
5261 CMP. TEST 103 1 to 8 WARNING The setting of formula
number corresponding to the relevant code is improper.
(1)An unmeasurable test is
specified for compensation.
(2)A compensated test is
not included in the formula.
(3)In photometry assay for
compensated test, the electrolyte parameter is specified.
2 - 18
Call up calculation item screen and check the compensation formula on it.
Page 31
Alarm Control No.
5271 to 5356
Alarm Category
SERUM INDEXES?
104 1 to 36
Sub­code
(ch.)
Level Description Remedy
WARNING (1)Although the sub-code
corresponds to the serum index measurement test, the rate-A assay is not assigned.
(2)Although the sub-code
corresponds to the serum index measurement test and sample blank is to be corrected, reagent 2 discharge is specified. (Analysis does not start.)
5431 ON BOARD? 106 1 WARNING There is no measurable
channel. There is no channel for
which necessary reagent has been prepared.
(Analysis does not start.)
5441 REAG.POS? 107 1 to 38WARNING (1)The reagent position
specified for a photometry assay is also specified for other photometry assay.
(2)The same reagent
position is specified for both carry-over cleaning agent and photometry assay or ISE test.
(Analysis does not start.)
5481 CLB(IS)
POS.
108 1 WARNING When ISE is provided,
calibrator or control positions are set at 55 to 57.
(Analysis does not start.) 5511 to 5516
ACI ERROR 114 1 to 6WARNING Barcode IC malfunctions.
1:Unassigned
2:Unassigned
3:Sample
4:Unassigned
5:Unassigned
6:Transfer
(cont’d)
Check parameter for serum indexes.
Check each parameter.
Check each reagent.
Check the position for control calibration.
Replace RSDIST PC board.
2 - 19
Page 32
Alarm Control No.
5561 to 5568
5571 to 5578
Alarm Category
FD-WRITE? 119 1 WARNING A hardware error has
119 2 WARNING A hardware error has
119 3 WARNING A hardware error has
119 4 WARNING A hardware error has
119 5 WARNING A hardware error has
119 6 WARNING A hardware error has
119 7 WARNING A hardware error has
119 8 WARNING A hardware error has
FD READ? 120 1 WARNING A hardware error has
120 2 WARNING A hardware error has
120 3 WARNING A hardware error has
120 4 WARNING A hardware error has
Sub­code
Level Description Remedy
occurred in writing the
routine sample
measurement data.
occurred in writing the stat
sample measurement data.
occurred in writing the
control sample
measurement data.
occurred in writing the
individual or cumulative
alarm information.
occurred in writing the
parameter data.
occurred in execution of FD
formatting, copying into FD
or FDD cleaning.
occurred in writing the cell
blank data.
occurred in writing the
routine sample test
selecting information.
occurred in reading the
routine sample
measurement data.
occurred in reading the stat
sample measurement data.
occurred in reading the
control sample
measurement data.
occurred in reading the
individual or cumulative
alarm information.
(cont’d)
(1)Clean the FD. (2)Replace the FD
with a new one.
(3)Replace the FD
drive.
Refer to alarm category No. 119.
2 - 20
Page 33
Alarm Control No.
5571 to 5578
5581 FD NOT
5601 FD
5621 to 5625
Alarm Category
FD READ? 120 5 WARNING A hardware error has
INSERTED
PROTECT PRINTER 125 1 WARNING Power supply is turned off
Sub­code
120 6 WARNING A hardware error has
120 7 WARNING A hardware error has
120 8 WARNING A hardware error has
121 1,2 WARNING System disk is not set in
123 1,2 WARNING A write-protected disk is
125 2 WARNING Paper has run out or the
125 4 WARNING A hardware error has
125 5 WARNING A time-out error of the
Level Description Remedy
occurred in reading the
parameter data.
occurred in reading the
channel assignment.
occurred in reading the cell
blank data.
occurred in reading the
control parameter.
drive 1 or data disk is not
set in drive 2.
inserted.
or the connector is
disconnected.
printer head has risen.
occurred on the printer.
printer has occurred.
(cont’d)
Refer to alarm category No. 119.
Insert the relevant disk.
Unprotect the disk.
Check the power supply or connector.
Set paper or lower the printer head.
Check the printer cable.
(1)Check the
printer cable.
(2)Replace the
printer.
2 - 21
Page 34
Alarm Control No.
5631 to 5643
Alarm Category
Sub­code
Level Description Remedy
SYSTEM I/F 126 1 WARNING A reception time-out error
has occurred.
126 2 WARNING A transmission time-out
error has occurred.
126 3 WARNING A BCC error or checksum
error has occurred.
126 4 WARNING A parity error has occurred.
126 5 WARNING A framing error has
occurred.
126 6 WARNING An overrun error has
occurred.
126 7 WARNING Frame error
126 8 WARNING Text length error
126 9 WARNING Function character error
(cont’d)
(1)Check the cable
of system I/F.
(2)Check the
contents of communication trace.
(3)Check the
contents of communication by line analyzer.
(4)Check if a
change has occurred in system parameters.
126 10 WARNING Sample information error
126 11 WARNING Test selecting information
error
126 12 WARNING Comment information error
126 13 WARNING Reception cannot continue
up to the end code because
an illegal character is
received from the host.
Example)A null code is
received from the host.
5681 BATH
EXCHANGE FAILURE
130 1 WARNING The start key has been
pressed despite failure in
incubation bath water
exchange. 5696 PANEL I/F 140 1 WARNING An error has occurred in
LCD display module
communication.
('Communication error'
appears on the LCD
display.) 5701 REAGENT? 141 1 to 36WARNING Either reagent positions R1
to R3 or reagent volume
alone is "0" (no
specification).
Do incubation bath water exchange again.
(1)Check the
communication cable of LCD.
(2)Replace the
LCD.
Check and correct the contents of parameters R1 to R3.
2 - 22
Page 35
Alarm Control No.
5771 CELL C. O. 145 1 to 10WARNING (1)More than 2 types of
5821 ISE C. O. 146 1 to 40WARNING R1 type is not specified for
Alarm Category
Sub­code
Level Description Remedy
carry over evasion (cell) are specified for 1 test.
(2)Sub-codes (1 to 10)
signify the evasion types.
ISE test of Reagent Probe
Carry Over Evasion.
(cont’d)
Check and correct
the carry over evasion (cell).
Check and correct the Reagent Probe carry over evasion.
2 - 23
Page 36

2.1.1 LCD Display Alarm

Output of Boot Error
Boot error is output to the console and the buzzer for small-size automatic analyzer (beeper is used). Output is issued unconditionally to the console whenever connected. For the small-size automatic analyzer, on the other hand, either of the destinations shown below is selected depending on system. For selection method, refer to (3).
(1) Output to Console
Output to the console always uses a log message. This is because output cannot be issued in the EAT format within a period from system start to EAT task start. Upon output, a message is sent out line by line by scroll-up method as in the logging message of VxWorks. Note that a line is fed before and after a message. Output format is shown below. For contents of output, refer to (4).
(2) Buzzer Output for Small-Size Automatic Analyzer
The kinds of output are the same as the three kinds in the small-size immunological system. Each output has a distinctive tone at the frequency indicated below.
1) Self Test Error : 50 Hz
2) Loading Error : 250 Hz
3) OS Initial Error : 1 Hz Sounding : Continuous
(3) Distinction in Error Message Output
For distinction in error output of small-size automatic analyzer, the configuration register of a flash memory is used.
According to the readout value of this register, output destination is distinguished as shown below. The value is set by hardware. (No setting is required in software.)
Table 2-1 Flash-Memory Configuration Register
(0xf2000b)
Value Meaning
0x01 Output to small-size automatic analyzer Bus error Other
2 - 24
Page 37
(4) Contents of Output
Output for the small-size automatic analyzer comes in 3 kinds described above. So, the kinds of console output are listed below.
Table 2-2 Kinds of Console Message Output
Error Output Message
Self-test error 1. Self Test Error (0xXX)
XX: Self-test error code
Boot error 1. Rom Uncompress Error
2. F/D Boot Error (0xXXXXXXXX)
3. FROM Boot Error (0xXXXXXXXX) XXXXXXXX: Boot error code (Refer to Section 5.)
OS initial error 1. OS Initial Error (Vect = 0xXX,PC=0xXXXXXXXX)
Vect = 0xXX: Vector No. PC = 0xXXXXXXXX: Program counter Note that EAT output is issued when possible.
2 - 25
Page 38

2.2 Motor Control Alarms

Alarm Code
1 - 1 (3) - 4) 6 - 9 (4) - 5) 17 Unused
2 Unassigned 10 (5) - 6) 3 (4) - 5) 11 (7) - 8) 18 - 1 (2) - 3) 4 (2) - 2)ž3) 12 (6) - 7) (3) - 4)
5 (2) - 2)ž3) 14 Unassigned (2) - 2) 6 Unassigned 7 - 1 (2) - 3) (3) - 4)
7 (4) - 5) (3) - 4) 19 Unused 8 (8) - 9) (9) - A 9 Unassigned (10) - A 20 Unused 10 Unassigned 2 (2) - 3)
2 - 1 Unused 3 (3) - 4) 21 Unused
2 4 (2) - 3) 3 (10) - C 22 - 1 (1) - 1) 4 7 - 5 (1) - 1) 2 (4) - 5) 5 (2) - 2) 23 Unused 6 (9) - B 7 (10) - B 24 - 1 (1) - 1) 8 8 Unused 2 (4) - 5) 9 25 Unused 10 9 Unused
3 - 1 (3) - 4) 26 - 1 (1) - 1)
2 (4) - 5) 10 Unused 2 (4) - 5) 3 3 4 11 Unused 4 5 6 (OP) 12 Unused 7 8 13 Unused 9
10 14 Unused 4 Unused 5 - 1 (2) - 3) 15 - 1 (1) - 1)
2 (2) - 2) 3 Unassigned
3 (3) - 4) 4 Unassigned
4 (2) - 2) 16 - 1 (3) - 4)
5 (1) - 1) 2 (6) - 7)
6 Unassigned 3 (4) - 5) 6 - 1 (3) - 4) 4 (70 - 8)
2 (3) - 4) 5 (3) - 4)
3 (5) - 6) 6 (4) - 5)
4 (6) - 7) 7 (7) - 8)
5 (4) - 5) 8 (8) - 9)
6 (4) - 5) 9 Unassigned
7 (7) - 8) 10 Unassigned
8 (3) - 4)
Check Procedure
(3) - 4) 13 (8) - 9) 2 (1) - 1) (3) - 4) 15 Unassigned 3 (2) - 3)
(3) - 4) 2 (4) - 5) (*) : OPTION
Alarm Code
Check Procedure
Alarm Code
Check Procedure
2 - 26
Page 39
2.2.1
1. For items other than 7 and 8 , check whether the motor remains running even after timeout.
NOTE:
If so, it should be identified as a
motor time out error.
2. Operation check method for 2-pitch returning of sample disk.
3. Item 10 ; Operation check method for 2-pitch feed of sample disk.
4. Item 9 ; Check at resetting.
2 - 27
Page 40
Notes:
1. In check procedure other than 7 and 8 , motor running status is checked after time-out. When running, a motor time-out error occurs.
2. Operation check procedure at 2-pitch return of the sample disk.
3. 10 is the operation check procedure at 2-pitch feed of the sample disk.
4.
9 is the check at resetting.
.
2 - 28
Page 41

2.3 Parameter Check

Parameter check is carried out on the channel for which test is selected on the CHANNEL ASSIGNMENT screen at start of analysis.

2.3.1 Processing Flow

(1) At input of START key in STANDBY status.
Twin test simultaneous analysis parameter check
Check result?
NG
STOP
Analytical method
OK
check
Calibration parameter Serum index analytical check method check
Volume check Reagent relationship check
1)
at carry-over cleaning
Upper/lower limit value CH, ACTIVATE check check
Test-to-test Check result? compensation check
Analytical mode check
1)
OK
NG
START
STOP
2 - 29
Page 42

2.3.2 Details of Parameter Check

Designation
No.
of Check
1 Check of
parameters for twin test simultaneous analysis
Details of Check
The following are checked for measurable (level 1, Note 1) photometry tests.
(1) In case of twin test
simultaneous analysis, the opposite test must be specified properly.
Two tests must form a unique pair, and one of them alone must designate the other.
(2) In case of other than
twin test simultaneous analysis, tests must not be paired.
(3) In case of twin test
simultaneous analysis, two tests must be identical in all of the following parameters.
Data mode
STD POS.
STD S. VOL
Assay code
Reaction time
Sample volume
Reagent volume
Calibration type
Calibration point
Span point
Alarm
Display Subdivision
TWIN TEST? 1 to 37
(CH No.)
Remarks
Details of this check are given in "Details of twin test simultaneous analysis" in 2.3.3.
In case of twin test simultaneous analysis, an alarm is issued if one of two tests is not registered or the manual mode is selected.
NOTE:
1. The term 'measurable' indicates either of the following two levels.
Level 1: Test registered
and manual mode not selected
Level 2: Same as above
and reagent provided
2 Assay code
check
The following are checked for measurable (level 1) photometry tests. (1) Relationship between
assay code and photometric point (check of photometric point input and input range) Refer to the analytical method table in 1.1.1.
CHEM PARAM?
2 - 30
1 to 37 (CH No.)
Page 43
Designation
No.
of Check
2 Assay code
check
3 Calibration
parameter check
Details of Check
(2) Photometric points after
the specified reaction time must not be set. Refer to Note 6 in "analytical method
table" of 1.1.1. The following are checked for measurable (level 1) photometry tests. (1) Relationship between
assay code and
calibration type
Refer to "relationship
between calibration type
and analytical method"
in 2.1. (2) Entry must be made for
CALIB. POINTS
necessary for calibration
type.
Refer to "output by each
calibration method and
check table" in 2.7. (3) Entry must be made for
STD POS. necessary
for calibration.
For STD specified by
measured STD No. in
"output by each
calibration method and
check table" of 2.7, it is
checked whether STD
POS. is input or not.
Alarm
Display Subdivision
CHEM PARAM?
1 to 37 (CH No.)
CLB. PARAM? 1 to 37
(CH No.)
(contd)
Remarks
NOTES:
1. Checked against the specification of calibration test selecting information.
2 - 31
Page 44
Designation
No.
of Check
3 Calibration
parameter check
4 Volume
check
Details of Check
(4) The input value for
SPAN POINT must be normal.
When calibration type is LINEAR (2 to 6-point) or LOGIT­LOG (3P or 4P)
1) The above input
value must not equal 0.
2) The above input
value must not exceed that for CALIB. POINTS.
(5) Relationship between
calibration type and calibration method Refer to "output by each calibration method and check table" in 2.7. (Note 2)
(6) STD CONC. must be
set in ascending order. Check is made only for necessary STD POS. Note that isozyme STD (3) and (4) are excluded. "=" is not regarded as in ascending order.
(7) When '99' is entered for
POS. of STD (1),
CONC. must be 0. The following are checked for measurable photometry tests. (1) The total liquid volume
up to the final
photometric point must
be 500 µL or less. (2) At least one of R1 to R3
volumes must not be 0. (3) The reagent volume
having a timing behind
the reaction time is not 0. (4) The minimum liquid
volume must be at least
250 µL.
Alarm
Display Subdivision
CLB. PARAM? 1 to 37
(CH No.)
VOLUME ? 1 to 37
(CH No.)
(contd)
Remarks
NOTES:
2. Checked against the specification of calibration test selecting
information.
When reagent volume = 0, the relevant reagent is not used.
2 - 32
Page 45
Designation
No.
of Check
5 Test-to-test
compensa­tion check
6 Serum index
analytical method check
7 Cell carry
over
8 ISE carry
over
Details of Check
For formula No. where compensated test is measurable: (1) The compensation test
must be measurable. In addition, when the compensation test is already registered as a compensated test in any preceding formula No., the check result for the formula No. must be OK.
(2) The compensated test
must be on the right side of the formula.
(3) When the compensated
test is a photometry test, no ISE test must be registered for the compensation test.
(1) The assay code for tests
for serum index measurement must be RATE-A. This check is made only when the tests are measurable.
(2) In the RATE-A test with
serum indexes, no R2 must be specified when sample blank correction is carried out.
(1) More than 2 types of
carry over evasion (cell) are specified for 1 test.
(2) Sub-codes (1 to 10)
signify the evasion types.
R1 type is not specified for
ISE test of Reagent Probe Carry Over Evasion.
(contd)
Alarm
Remarks
Display Subdivision
CMP. TEST? 44 to 51
(FORMULA No.)
This check is not performed in case of ORIGINAL ABS.
S. INDEXES? 1 to 37
(CH No.)
CELL. C. O. 1 to 10 Check and correct
the carry over evasion (cell).
ISE C. O. 1 to 40 Check and correct
the Reagent Probe carry over evasion.
2 - 33
Page 46

2.3.3 Details of Twin Test Simultaneous Analysis

NG Note 2
CH = 1 to 37 Assay code P: Designates OK
for twin test opposite test.
Note 1 simultaneous Q: Designated by NG Note 2
P.Q
P.Q
P.Q
analysis opposite test. OK
P.Q
Designated by one test alone
Check result OK
Identical in all parameters with opposite test Note 3
OK
Not paired
NGNote 2
NG Note 2
OK
NG Note 2
NOTES: 1. Unmeasurable channels are excluded.
However, in twin test simultaneous analysis, alarm occurs when either one of the two tests is unmeasurable.
2. Alarm is registered with a channel No. assigned for subdivision.
3. Refer to "Details of Parameter Check" in 2.3.2.
2 - 34
Page 47
2 - 35
Page 48

2.4.2 Data Alarm Code List

No. Data Alarm Remarks
Output String ISE
PRINTER C
R T
1
ADC abnornal
2
Cell blank abnormal
3
Sample short
4
Reagent short
5
Absorbance over
6
Prozone error
7
Reaction limit over at all points
8
Reaction limit over except 1 point
9
Reaction limit over except 2 or 3 points
10
Linearity abnormal at 9 points or more
11
Linearity abnormal at 8 points or less
12
Standard 1 absorbance abnormal
13
Duplicate error
14
STD error
15
Sensitivity error
16
Calibration error
17
SD error
18
Noise error
19
Level error
20
Slope abnormal
21
Internal standard concentration abnormal
22
Sample value abnormal
23
Test-to-test compensation error
24
Test-to-test compensation disabled
25
Calculation test error
26
Overflow
27
Calculation disabled
28
Expected value high limit over
29
Expected value low limit over 30 Electrode preparation PREP. R 31 SD absorbance over >AMAX > >
ADC? CELL? SAMPLE REAGN ABS? ∗∗∗∗∗P LIMT0
LIMT1 LIMT2 LIM. LIM.8 S1ABS? DUP
STD? SENS CALIB SD? NOISE LEVEL SLOPE? I.STD
R.OVER CMP.T
CMP.T! CALC?
OVER ??? H
L
A Q V T Z P I
J K W F
N L
& C
M %
0 X
Photometry
Assay
S.
I/FRo
u t i n e
O
A
O
Q
O
V
O
T
O
Z
O
P
O
I
O
J
O
K
O
W
O
F H U
S Y B G N L E
O
D
O
& C
O O O
M
O
%
O
0 X
O O O
S
C
S
R
S
C
S
t
o
T
o
t
o
T
a
n
D
u
a
n
D
t
t
t
t
t
r
i
r
o
n
o
l
e
l
O
O
O
O
O
O
O
O
O
O
O
O O O O O
O O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
Data may become blank
space.
Data may become blank
space.
Prozone value is output to the
printer only when the monitor is in the real time mode. ∗∗∗∗∗ indicates a prozone
O
O
O O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
O
value (5-digit).
O O O
O O O
Data becomes blank space.
O
Data becomes blank space
Data becomes blank space.
May concur with other alarm.
May concur with other alarm.
O
Data becomes blank space.
BM only
NOTES: 1. If any data caused multiple data alarms, the alarm registered first will be output.
2. When [Specify] is entered for measured value space parameter in the start condition screen.
2 - 36
Page 49

2.4.3 Data Alarm Codes

No. Data Alarm Printer S. I/F Description Remedy
ADC abnormal ADC? A The ADC value of main or
sub wavelength (only main wavelength in single wavelength photometry) is
zero. Cell blank abnormal
Sample short SAMPL V Before sample aspiration,
Reagent short REAGN T Before reagent aspiration,
CELL? Q (Photometry assay only)
Two or more of the four
passed cell blank values to
be used for CELL BLANK
are abnormal.
Note: Abnormal when the
difference from the reference value (value measured with cell blank function on the maintenance screen) is ± 0.1 Abs or more.
the presence/absence of
sample is checked and it is
found that the sample is
absent.
the presence/absence of
reagent is checked and it is
found that the reagent is
absent.
Perform measurement again.
Turn off the power switch, and then turn it on.
Perform cell blank measurement once a week or after replacement of the light source lamp or reaction cuvette.
Confirm the adequate volume of HITERGENT. After taking a proper measure, exchange incubation bath water once.
Rinse or replace the reaction cuvette and perform cell blank measurement.
After washing the light transmitting window in the incubation bath, perform cell blank measurement.
Inject the sample in a volume of expected consumption + 50 µL or more into a standard cup.
Insert the lead wire.
Prepare and set reagent
newly.
Insert the lead wire.
2 - 37
Page 50
No. Data Alarm Printer S. I/F Description Remedy
Absorbance over ABS? Z (Photometry assay)
The absorbance value to be used for calculation after cell blank correction exceeds 3.3 Abs. The absorbance is checked for every wavelength.
Prozone error
*****P ('*****' indicates a prozone value.)
P (Photometry assay only)
In 1-point or 2-point assay with prozone check, the prozone error is indicated if 'prozone' is detected. Prozone check is performed by comparing the prozone value (hereafter referred to as PC value) obtained from calculation mentioned below with the prozone limit value preset on the chemistry parameter screen. For the lower prozone limit, the prozone error is indicated if the PC value is smaller. For the upper prozone limit, the error is indicated if the PC value is larger. In the event of prozone error, the relevant PC value is also printed out (only in real-time monitor printing mode).
In 1-point & rate, rate-A or rate-B assay, check is not carried out for the interval to determine the absorbance change rate if an ascending reaction setting is made for that interval.
Dilute the sample or reduce its volume, and then perform measurement again.
Set the sample correctly.
Prepare the reagent
again.
Prozone check values are printed out only in the real-time monitor printing mode.
Delution or retest with decreased volume is performed.
For no-check, enter ‘-32000’ (lower limit) at “Prozone limit value” on the parameter setting menu screen 1.
(contd)
2 - 38
Page 51
No. Data Alarm Printer S. I/F Description Remedy
Ep
Ep
Prozone error
*****P ('*****' indicates a prozone value)
P Shown below are the
expressions for calculation of the PC value. Assuming that the preset photometric points are P1 and P2 and the difference in absorbance between two wavelengths at photometric point P is E:
(1) In 1-point assay
PC value =Ep2, p
Ep2, p
2-1
1-1
-k
k: Liquid volume
correction factor
Note that k = 1 when
1 ≤ P1, P2 ≤ 5 or 6 ≤ P1, P2 ≤ 16 or 17 ≤ P1, P2 ≤ 33 or
34 ≤ P1, P2 ≤ 73. Prozone check is not carried out if P2 = 0.
(contd)
(2) In 2-point assay
PC value =
2 3
P2' P3
Ep Ep
4 3
P4 P3
Note that prozone check is not carried out if P3 = 0 or
|Ep4 - Ep3| ≤ 100 × 10
- 4
Abs.
When P2' = 0, P2 is used.
In either case of (1) and (2), prozone check is not made for STD (1) measurement.
2 - 39
Page 52
No. Data Alarm Printer S. I/F Description Remedy
Reaction limit over
(Photometry assay only) In 2-point rate, 1-point & rate, rate-A or rate-B assay, the main wavelength absorbance at the photometric point to be used for calculation exceeds the reaction limit value (value
This alarm is issued whenever the input photometric range values l and m for calculation of the absorbance change rate do not satisfy l + 2 < m.
obtained after automatic correction of input reaction limit value). The error condition varies depending
All points NG
Only one point OK
Only two or three points OK
LIMT 0
LIMT 1
LIMT 2
on the number of photometric points within the reaction limit
I
range as shown below. (1) The reaction limit is
exceeded at all points in the input photometric
J
range.
(2) The reaction limit is
exceeded at the second and subsequent points
K
excluding the first point.
(3) The reaction limit is
exceeded at the third and subsequent points excluding the first two points, or at the fourth and subsequent points excluding the first three points.
(Caution) In 2-point rate assay, check is made using not the input photometric range values l and m alone but all photometric points between l and m.
Dilute the sample or reduce its volume, and then perform measurement again.
Prepare the reagent again.
Check for leakage or clogging of the reagent pipettor.
Input correct values on parameter registration menu.
(contd)
2 - 40
Page 53
No. Data Alarm Printer S. I/F Description Remedy
Linearity abnormal
LIN.
Number of photometric points in reaction limit level range 9
(Photometry assay only) In 1-point & rate, rate-A or
W
rate-B assay, the absorbance at each photometric point to be used for calculation of the absorbance change rate does not satisfy the following relational requirement.
Make sure the sample does not contain dust, etc.
Dilute the sample and measure it again.
If the stirring motor does not rotate normally, report to the servicemen.
4 ≤ Number of photometric points in reaction limit level range 8
LIN. 8
E1− ∆E
Linearity limit value
F
Where, E: Absorbance change rate determined from absorbance at each photometric point in reaction limit level range by least squares method
E
b
× 100
The light source lamp
should not be used beyond 750 hours.
Replace the light source lamp and perform cell blank measurement.
E1: Absorbance change rate in first half
Eb: Absorbance change rate in second half
Assuming that the number of photometric points in the reaction limit level range is N, E1, Eb and linearity limit value can be represented as shown below.
LIMIT8 and LIN.LIMIT values are both fixed.
(Unit: %,
(1) When N 9
Linearity limit value LIMIT 8
value: LIN.LIMIT = 10, LIMIT8 = 30)
(contd)
2 - 41
E f
E
b
Page 54
No. Data Alarm Printer S. I/F Description Remedy
4 ≤ Number of photometric points in reaction
LIN. 8 F
(2) When 4 ≤ N ≤ 8
Linearity limit value LIN. LIMIT
limit level range 8
E f
E
b
Note that linearity check is not carried out in the following cases.
The number of photometric points in the reaction limit level range is three or less.
|E| 60 (× 10e - 4ABS/min)
|Ef - E
|≤ 60
b
(× 10e - 4ABS/min)
(contd)
Standard 1 absorbance abnormal
S1ABS? H (Photometry assay) (Note 1)
In calibration, the mean value of two measured absorbance values of STD (1) is not within the specified standard 1 absorbance range (input value).
Value is absorbance with End-point Assay, in the case of late assay, is main wave O length first absorbance.
Duplicate error DUP U (Photometry assay)
In calibration, this error is indicated if the difference in absorbance (or absorbance change rate) between the first and second measurements of STD (i) is larger than the DUPLICATE LIMIT (input value).
(i = 1 to N: N indicates the number of standards.)
Prepare the standard properly.
Set the standard properly.
Unless check is desired, input a value within
- 32000 to 32000 for "standard 1 absorbance range" on the parameter registration menu 1 screen.
Replace the seal piece of pipettor.
Check fastening of joints.
Unless check is desired, input 32000 for "duplicate limit absorbance" on the parameter registration menu 1 screen.
2 - 42
Page 55
No. Data Alarm Printer S. I/F Description Remedy
STD error STD? S (Photometry assay)
(1) In calibration, any one of
the following alarms is encountered with the measured STD absorbances:
ADC abnormal, cell blank abnormal, sample short, reagent short, absorbance over, reaction limit over, linearity abnormal, prozone error, duplicate error, calculation disabled and standard 1 absorbance abnormal.
(2) In calibration, calculation
is disabled before completion.
(3) In non-linear calibration
(EXPONENTIAL and Logit-Log5P), an extreme value is found.
(4) After execution of non-
linear calibration (SPLINE), the result of extreme value/inflection point check is NG.
The parameters on the screen and FD are not updated.
(contd)
(ISE) Calibration is invalid. (Any one of the following
alarms is encountered: ADC abnormal, sample short, calculation disabled, noise error and level error.)
2 - 43
Page 56
No. Data Alarm Printer S. I/F Description Remedy
Sensitivity error SENS Y (Photometry assay)
Sensitivity check is carried out only for linear (2 to 6-point), non-linear or isozyme P calibration.
This error is indicated if the difference in mean absorbance between STD (1) and STD (N) is smaller than SENSITIVITY LIMIT (input value).
N: Linear (2-point)/
isozyme P calibration ..... 2
Non-linear/linear (3 to 6-
point) calibration ..... 2 to 6
(input value for SPAN POINT)
Note, however, that N = 2 when CALIB. POINTS = 2 in non-linear LOGIT-LOG (3P)/(4P) calibration.
The parameters on the screen and FD are not updated.
Set the standard solutions and reagent properly.
Prepare the standard solutions newly.
Check the sample syringe for leakage, clogging, etc.
Unless check is desired, input 0 for "sensitivity limit absorbance" on the parameter registration menu.
(contd)
For the mean absorbance of STD (1) in span calibration, the following data is used.
Linear ..... Previous S1 ABS
Non-linear ..... Previously
updated mean absorbance
Sensitivity check is not made in R.B. calibration.
SD error SD? G (Photometry assay)
This error is indicated upon completion of non-linear calibration or if the SD value in linear (multi-point) calibration is larger than SD LIMIT (input value).
Set in correct concentration series.
Replace the seal piece of pipettor.
Check fastening of joints.
Unless check is desired, input 999.9 for "SD limit absorbance" on the parameter registration menu.
2 - 44
Page 57
No. Data Alarm Printer S. I/F Description Remedy
Calibration error CALIB B (Photometry assay)
In linear (2 to 6-point) or isozyme P calibration, the calibration condition is checked at calculation of parameter K.
This error is indicated if there is a difference of ±20% or more between the current and previous K values.
Same as in SENS
Pay attention to
storage method and the time period of placement on the sample disk.
If the result is satisfactory, operation can be continued. After
(ISE) The calibration concentration or
slope level for display does not satisfy the following expression.
|Previous value - current value|
(Previous value + current value )/2 ×100% > COMPENSATE LIMIT
Noise error NOISE N (ISE)
This error is indicated if the difference between the maximum and minimum potentials is within the following range at three of the five measuring points for each test (on internal standard or sample).
measurement, record parameters on the maintenance menu to store the K value.
Set reagent and perform ISE priming (with internal standard solution and diluent) once.
Make sure O-ring is fitted to each electrode and its holder and it is not
Na : 0.7mV < |FIV(2) - FIV(4)| K : 1.0mV < |FIV(2) - FIV(4)| Cl : 0.8mV < |FIV(2) - FIV(4)|
fitted doubly. After reattaching, perform ISE priming (with reference electrode solution).
Replace the tube and perform ISE priming (with reference electrode solution) once.
Clean the waste solution drain path.
Clean the syringe and plunger and replace the seal piece.
(contd)
2 - 45
Page 58
No. Data Alarm Printer S. I/F Description Remedy
Level error LEVEL L (ISE)
This error is indicated if the mean potential is outside the following range at three of the five measuring points for each test (on internal standard).
Set reagent and perform ISE priming (with reference electrode solution) twice.
Replace the electrode and perform ISE priming (with reference
Na: -90.0mV ≤ EAV ≤ -10mV
OK
K : -90.0mV ≤ EAV ≤ -10mV
OK
Cl : 100.0mV ≤ EAV
180.0mV ⇒ OK
electrode solution) once.
Retighten the nipple. Or after replacing the rubber packing, perform ISE priming (with reference electrode solution) once.
Confirm the proper combination of tube and reagent.
Plug in the cord properly.
Slope abnormal SLOPE? E (ISE)
(1) The slope level for display
is within the following range.
Na, K : SLOPE < 45.0mV, Cl : SLOPE > -35.0mV
Replace the electrode.
Remove the probe and
correct clogging.
Set properly.
Confirm the proper
combination of tube and reagent.
(2) Electrode response is
degraded. (Carry-over rate is as
shown below.) Na : 0.232 < A K : 0.160 < A Cl : 0.490 < A
Electrode preparation
PREP. R Upon calibration, the slope
value is within the following range.
Make sure that the standard solution and reagent are set properly.
Make sure that the
Na, K : 45.0mV ≤ Slope value
49.9mV or
68.1mV ≤ Slope value
Cl : -39.9mV ≤ Slope value
-35.0mV or
-68.1mV ≤ Slope value
standard solution is free from concentration or deterioration.
Make sure that the electrodes (Na, K, Cl) are within their guaranteed life.
(contd)
2 - 46
Page 59
No. Data Alarm Printer S. I/F Description Remedy
Internal standard concentration abnormal
I. STD D (ISE)
The internal standard concentration (C(IS)) is within the following range.
Na : C(IS) < 120.0mEq/L or
Confirm monthly flow path washing.
Replace the diluent and internal standard solution.
160.0mEq/L < C(IS)
K : C(IS) < 3.0mEq/L or
7.0mEq/L < C(IS)
CL : C(IS) < 80.0mEq/L or
120.0mEq/L < C(IS)
Calculation test error
CALC?
%
Any data alarm other than shown below is indicated for the test to be used for
Check the data alarm name and take a proper measure.
calculation. Calculation disabled, test-to-
test compensation disabled, expected value over
Overflow OVER
O
Concentration value (or activity value) cannot be output within the specified range of digit
The data is left blank.
Prepare appropriate
standard solution.
count.
Calculation disabled
???
X
In process of calculation, the denominator becomes zero.
An overflow occurs in logarithmic or exponential calculation.
In isozyme Q-channel concentration calculation, the data alarm of 'calculation disabled' is indicated for the isozyme P-channel data or the isozyme P channel is not measured.
The data is left blank.
Determine the cause of
failure to color development such as improper kind of standard solution, wrong set position and clogging of sample probe.
Dilute the sample or reduce its volume and perform analysis again.
Calculation for a calculation test has been attempted with the data having a data blanking alarm.
Expected value over
L H
The test result is outside the expected value range (outside mean value ± 2SD in case of a control sample).
Smaller than the lower limit
– –
value
Larger than the upper limit
This alarm is not
indicated for serum index.
Correct setting on the parameter registration menu 1 screen.
value
(contd)
2 - 47
Page 60
No. Data Alarm Printer S. I/F Description Remedy
Sample value abnormal
R. OVER
&
(ISE) The sample concentration (C(S)) is within the following range.
Na : C(S) < 10.0mEq/L or
C(S) > 250.0mEq/L
K : C(S) < 1.0mEq/L or
C(S) > 100.0mEq/L
Dilute the sample and analyze it again.
Direct measurement is impossible. Utilize standard addition method, etc.
Remove the probe and correct clogging.
Cl : C(S) < 10.0mEq/L or
C(S) < 250.0mEq/L
Test-to-test compensation error
CMP.T
C
(1) In test-to-test
compensation calculation, any data alarm other than
Check the channel data used for test-to-test compensation.
shown below is indicated for the compensation data.
(2) In isozyme Q-channel
concentration calculation, any data alarm other than shown below is indicated for the isozyme P-channel concentration. Calculation disabled, test­to-test compensation disabled, overflow, random error, systematic error, QC error, expected value over
Test-to-test compensation disabled
CMP.T!
M
(1) In process of calculation for
test-to-test compensation, the denominator becomes zero.
(2) The test to be used for test-
to-test compensation is not measured.
(3) Any test to be used for test-
to-test compensation has
The data is left blank.
Make sure masking is
not specified for the test to be used for compensation.
Check the calculation formula on the parameter registration menu 3 screen.
the data alarm of 'calculation disabled' or 'test-to-test compensation disabled.'
(4) Any compensation test has
the data alarm which leaves the data blank.
SD absorbance over
>AMAX
>
At standard concentration of
∞”, absorbance of sample or
absorbance change rate is over or the same compared with presumptive absorbance or absorbance change rate.
(contd)
2 - 48
Page 61

2.4.4 ISE Data Alarms

Alarm Output ISE
Priority Alarm Printout CRT S. I/F Remarks
Alarm on ISE data processing has 2 kinds shown below; calibration alarm and data alarm. (1) Calibration Alarm
1 STD error STD?
2 Slope
abnormal
3 Electrode
preparation
4 Internal
standard concentration abnormal
5 Calibration
error
< Key >
N : Routine sample measurement E : Stat sample measurement C : Control sample measurement STD : Calibration measurement
SLOPE?
PREP
I. STD
CALIB
Processing
N.E.C.R STD
S
E
R
D
B
×
× × ×
×
on Operation Monitor Screen
O O In calibration
measurement, this alarm is set at any of the data alarms in (2) (excluding data alarms 6 to
9).
O O
O O
O O
O O
2 - 49
Page 62
(2) Data Alarm
Priority Alarm Printout CRT S. I/F Remarks
1 ADC
abnormal
2 Sample short SAMPLE V V
3 Noise error NOISE N N 4 Level error LEVEL L L 5 Sample value
abnormal
6 Calculation
disabled
7 Test-to-test
compensation disabled
8 Test-to-test
compensation error
9 Overflow OVER O O
ADC? A A
R.OVER & &
???
CMP.T! M M
CMP.T C C
× ×
ISE Processing
N.E.C.R STD
O O O
O O
Alarm Output on Operation Monitor Screen
×
Registration is made on the operation monitor in ADC task.
At occurrence of this alarm, the 'calculation disabled' alarm is also set (for making output data blank).
O O O O O O O O O
O O
O
O
O
× × ×
× ×
× ×
Check is not performed in ISE data processing.
× × ×
× ×
ISE Check Data Alarm
O
×
×
O
2 - 50
Page 63
(3) Registration of Data Alarm "sample short"
Measured Sample
Other than STD (1) A
STD (1) A
NOTES: 1. A: Pre-detection is not made.
B: Pre-detection is made and at least either one of the following is encountered.
(i) The number of remaining pulses is zero. (ii) Abnormal fall detection is activated within ±2 mm of the cup bottom level recognized
C: Other than A and B
(Pre-detection refers to liquid level detection till a time point just before sample aspiration since start of probe fall.)
2.
O: Output
Alarm Issued in Sampling
(Note 1)
1st Time 2nd Time
B
C
B
C
Water discharge
in probe adjustment.
×: Space
O: Issued
3.
×: Not issued
Data Output
(Note 2)
– – –
– – –
×
O O
×
O O
O
Data Alarm
(Note 3)
O O
×
O O
× ×
2 - 51
Page 64

2.4.5 Alarm Check Method

(1) Photometry Assay Calibration Check
(a) SD check of approximate expression
When SD in the difference between the automatically generated calibration curve and the measured absorbance value in non-linear calibration is larger than "SD limit absorbance," comment SD? is printed. SD value is printed under the test name in the result of calibration. Unless check is desired, input 999.9.
(b) Duplication check
In measurement of reagent blank and standard solution, comment DUP is printed when the difference in absorbance between two measurements is larger than "duplicate limit absorbance." Unless check is desired, input 32000.
(c) Sensitivity check
When the difference in absorbance between reagent blank (STD1) and standard solution (having the maximum concentration if there are multiple standard solutions), comment SENS is printed. Unless check is desired, input 0.
(d) Standard solution 1 absorbance check
When the absorbance of reagent blank (standard solution 1) exceeds "standard 1 absorbance range," comment S1 ABS is printed. In an end-point assay test, the absorbance value on the left side of calibration result printout is checked and that on the right side is checked in a rate-assay test. Unless check is desired, input ­32000 to 32000.
2 - 52
Page 65
(2) Reaction Limit Level Check
When concentration or enzyme activity is abnormally high in a rate assay test, correct data is unobtainable because the substrate or coenzyme in reagent is consumed completely. Therefore, the upper or lower reaction limit absorbance is set for check . Check is made on the absorbance at the main wavelength alone.
< Relationship between Alarm Name and Photometric Point > When 4 points or more over the specified photometric range are within the reaction limit,
measurement is carried out normally. In case no point, 1 point or 2 points are within the reaction limit, a reference value is obtained depending on an absorbance change between the first two points. In case 3 points are within the reaction limit (with comment LIMT2), the value obtained depending on an absorbance change among the first three points is printed as a reference value.
ABS.
Input photometric range (with comment LIMT0)
ABS.
Input photometric range (with comment LIMT2)
ABS.
Reaction limit level
Time
Input photometric range (with comment LIMT1)
ABS.
Time
Input photometric range (with comment LIMT2)
Fig. 2-1 Reaction Limit Level Check
Time
Reaction limit level
Time
< Automatic Correction of Reaction Limit Level > The instrument corrects the input reaction limit level by adding an absorbance value due
to sample turbidity, etc. Reaction limit level = Input reaction limit absorbance value + (L1 - LB)
L1: Sample absorbance at photometric point 1 LB: Reagent blank absorbance at photometric point 1 When L1 - LB ≤ 0, automatic calibration will not be performed.
2 - 53
Page 66
(a) Electrolyte compensable range check.
When the ratio of change in calibrator concentration or slope value from the previous one is larger than the input value, comment CALIB is printed. Unless check is desired, input 200%.
(b) Calibration check
When calibration factor K has changed by 20% or more from the previous value, comment CALIB is printed (check value is fixed at 20%).
(c) STD check
If any of the following alarms occurs on calibration data, comment STD? is printed.
ADC abnormal (ADC?) Reaction limit over (LIMT0, 1, 2)
Cell blank abnormal (CELL?) Reaction linearity abnormal (LIN. or LIN.8)
Sample short (SAMPL) Duplicate error (DUP)
Reagent short (REAGN) Standard 1 absorbance abnormal
(S1ABS?)
Absorbance over (ABS!) Calculation disabled (???)
Prozone error (xxxxxP)
(d) Measure to be taken on printout of comment
When comment STD? or SENS is indicated, calibration curve will not be updated. Therefore, recalibration is necessary. In case of CALIB or SD!, sample measurement is allowed after making sure the result of calibration is normal. In this case, however, the result of calibration will not be saved automatically onto the floppy disk. Before turning off power supply, parameters should be recorded on the maintenance screen.
Table 2-1 Handling of Calibration Result with Comment
Printed Comment
STD? Not updated
SENS Not updated
CALIB Updated
SD ! Updated
Calibration Result (display on screen)
(previous result remains)
(previous result remains)
(to new result)
(to new result)
Alarm Name on Operation Monitor Screen
Calibration curve generation disabled (code 70-1 to 49)
Standard solution sensitivity abnormal (code 73-1 to46)
Calibration abnormal (code 70-1 to 49)
Calibration SD abnormal (code 72-1 to 46)
Automatic Saving onto Floppy Disk
The reagent blank (S1ABS) and calibration factor (K) of the relevant test are not saved automatically onto floppy disk.
2 - 54
Page 67
(3) Reaction Linearity Check
∆Af−∆Ab
In a rate assay test, the linearity in absorbance change is checked. Check value varies with the number of points (N) in photometric range.
Fig. 2-2 Reaction Linearity Check
< When N 9 >
The difference in absorbance change quantity between the first-half 6 points (5 sections) and the latter-half 6 points is obtained and then divided with the overall absorbance change quantity. When the result of this calculation exceeds the limit in linearity check, comment LIN. is printed together with the result of measurement.
A
× 100 > Linearity limit value.....LIN.
< When 4 ≤ N ≤ 8 >
The difference in absorbance change quantity between the first-half 3 points (2 sections) and the latter-half 3 points is obtained and then divided with the overall absorbance change quantity. When the result of this calculation exceeds the limit in linearity check, comment LIN.8 is printed together with the result of measurement.
9
Af
• ∆Af, ∆Ab and A in the above formula are all converted into absorbance change in a minute
In the following cases, reaction linearity is not checked.
9
Ab
− ∆
9
A
by the least squares method.
a) The number of photometric points (N) within the reaction limit is 3 or less ("reaction limit
b) Absorbance change in a minute is 60 × 10-4 Abs or less, or |Af - Ab| is equal to or
× 100 > Linearity limit value.....LIN.8
over" will occur).
smaller than 60 × 10-4.
2 - 55
Page 68
(4) Prozone Check
In immunological reaction, the absorbance of calibration curve falls at high concentrations so that correct data is unobtainable (this is called "zone phenomenon or prozone effect"). Therefore, prozone check is performed by the two methods below and a data comment is indicated when required.
< Antigen Readdition Method >
In 1-point assay, a small amount (approx. 50 µL) of sample containing antigen is readded for the second reagent (R2 to R4) to check the change in absorbance before and after addition (the dashed line in Fig. 2-3 indicates that absorbance falls due to excessive antigen).
< Reaction Rate Ratio Method >
In 2-point assay, check is performed according to the ratio of the initial reaction rate after addition of antiserum to the mean reaction rate.
Check Abs.
R1 (antiserum)
Rn (antigen)
Time
Fig. 2-3 Antigen Readdition Method
R1 (1st reagent)
Fig. 2-4 Reaction Rate Ratio Method
Rn (nth reagent) Time
2 - 56
Page 69
Absorbance for concentration calculation
Prozone check value (PC value)
Antigen Readdition Method
(1-point assay)
Al + A 2
l-1
Al + A 2
l-1
Ax =
Am + A
PC = -k
m-1
2
Reaction Rate Ratio Method
(2-point assay)
Am + A
Ax = -k
2
(Am - An)/(m-n)
PC = × 100
(Ap - An)/(p-n)
m-1
Al + A 2
l-1
< Judgment > In case of 'limit value - above,' comment xxxP (xxx indicates PC value) is printed when PC
value is larger than the limit value. In case of 'limit value - below,' the comment is printed when PC value is smaller than the limit value.
Prozone check will not be performed in the following cases. (1) In STD (1) measurement (2) |Ap - Am| < 100 × 10-4 Abs
2 - 57
Page 70
(5) ISE Calibration Alarm Check
Alarm Name Alarm Check Method (alarm setting condition)
Standard error Calibration is invalid.
(Any of the following alarms has occurred; ADC abnormal, sample short, calculation disabled, noise error and level error.)
Slope abnormal (1) The slope for display is within the following range.
Na, K : Slope < 45.0 mV Cl : Slope > -35.0 mV
(2) Electrode response is degraded (carry-over ratio is as given below).
Na : 0.232 < A K : 0.160 < A Cl : 0.490 < A
ISE prepare (1) The slope for display is within the following range.
Na, K : 45.0 mV ≤ Slope ≤ 49.9 mV or
68.1 mV ≤ Slope
Cl : -39.9 mV ≤ Slope ≤ -35.0 mV or
-68.1 mV Slope
(2) Electrode response is degraded (carry-over ratio is as given below).
Na : 0.154 < A K : 0.107 < A Cl : 0.330 < A
Internal standard concentration abnormal
Calibration abnormal
The concentration of internal standard solution (C(IS)) is within the following range.
Na : C (IS) < 120.0 mEq/L or 160.0 mEq/L < C (IS) K : C (IS) < 3.0 mEq/L or 7.0 mEq/L < C (IS) Cl : C (IS) < 80.0 mEq/L or 120.0 mEq/L < C (IS)
On each of the calibrator concentration and slope for display, the previous and current values are compared and the result exceeds the COMPENSATE LIMIT value.
Previous value - Current value
× 100 (%) > COMPENSATE Limit
(Previous value + Current value)/2
2 - 58
Page 71
(6) ISE Data Alarm Check
Alarm Name Alarm Check Method
ADC abnormal The result of ADC is abnormal. (Checked in ADC task) Sample short Sample volume is inadequate. (Checked in control task) Noise error This alarm is issued when a difference between maximum and minimum
potential values at three out of five measurement points in each test is within the following range (on internal standard and sample).
Na : 0.7 mVFIV (2) - FIV (4) K : 1.0 mVFIV (2) - FIV (4) Cl : 0.8 mVFIV (2) - FIV (4)
Level error This alarm is issued when a difference between maximum and minimum
potential values at three out of five measurement points in each test is within the following range (on internal standard).
Na : -90.0 mV ≤ EAV ≤ -10 mV ⇒ OK K : -90.0 mV ≤ EAV ≤ -10 mV ⇒ OK Cl : 100.0 mV ≤ EAV ≤ 180.0 mV ⇒ OK
Sample value abnormal The sample concentration (C(S)) is within the following range.
Na : C (S) < 10 mEq/L or C (S) > 250 mEq/L K : C (S) < 1 mEq/L or C (S) > 100 mEq/L Cl : C (S) < 10 mEq/L or C (S) > 250 mEq/L
Calculated disabled Due to zero division, log-X, etc.
2 - 59
Page 72

2.4.6 Check and Set Alarm of Each Data

(1) Calibration
Priority
Data Kind
Potential of internal standard solution
Low potential of standard solution
High potential of standard solution
Calibrator potential Na
Slope value (for display)
Concentration of internal standard solution
Calibrator concentration
Correction factor Na
Na
K
Cl
Na
K
Cl
Na
K
Cl
K
Cl
Na
K
Cl
Na
K
Cl
Na
K
Cl
K
Cl
1 2 3 4 5 6
Noise error Level error
ADC abnormal
Slope abnormal
Internal standard concentra­tion abnormal
Calibration abnormal
Calculatio n disabled
Sample short
Preparation abnormal
Calculation disabled
Calculation disabled
Noise error
Calibration abnormal
Level error
Calculation disabled
(2) Routine Sample Measurement (1st), Stat Sample Measurement (1st) and Control Sample
Measurement
Data Kind
Sample concentration
Priority
Na
K
Cl
1 2 3 4 5 6
ADC abnormal
Sample short
Noise error Level error Sample
value abnormal
Calculatio n disabled
If multiple data alarms concur, the one with the highest priority will be indicated.
2 - 60
Page 73

2.4.7 Details of Data and Alarm Outputs Resulting from Calibration

C3
disabled
Sample error factors
Standard solution LOW
Standard solution HIGH
Standard solution CALIB­RATOR
IS
L2
IS
L3
IS
M1
E
L2
E
L3
IS
M2
IS
M3
IS
C1
E
M2
E
M3
IS
C2
IS
C3
IS
C4
E
C2
E
ADC Noise Level
ADC
ADC
Sample short
Noise
Level
Level
ADC Noise Level
ADC Sample short Level
ADC Noise Level
ADC Sample short Level
Calculation error factors
Slope abnormal
Slope
IS concentration
Preparation abnormal Calibration abnormal Calculation disabled
Internal standard concentration Calculation disabled
Noise errror
Level error
ADC error
Sample short
Noise error
ADC error
Sample short
Noise error
ADC error Sample short Noise error
Level error
STD error
Slope abnormal Preparation
abnormal Calibration abnormal Calculation disabled
Internal standard concentration abnormal Calculation
Calibration abnormal Calcuration disabled
Calcuration disabled
IS potential
Low potential
High potential
Calibration potential
Slope
Internal standard concentra­tion
Calibration concentra­tion
Correction factor
Calibrator concentration
Correction factor
Calibration abnormal Calculation disabled
Calculation disabled
2 - 61
Page 74
2 - 62
Page 75
(1) List of Calibration Output Media
Output Medium
Output Data
Calibration curve parameters (S1ABS, K, A, B, C)
SD value Serum index blank CALIBRATION LIST Blank level Absorbance or absorbance
change rate Initial or final observance
CALIBRATION LIST
CRT
(screen name)
×
× × × ×
×
PRT S. I/F
× ×
CALIBRATION MONITOR
× ×
CALIBRATION MONITOR CALIBRATION MONITOR
NOTE: In plotting of measured absorbance values, the currently measured STD alone is
taken.
(2) Relationship between Alarm and Output in Calibration
Data Alarm Name
S1ABS error No No DUPLICATE error No No STD error No Yes SENSITIVE error No Yes CALIB error Yes Yes SD error Yes Yes No alarm Yes No
Updating of Screen Parameter
Screen Display of Alarm
Remarks
O
O
O
NOTE: For CALIB and SD errors, "∗" is output on logging for discrimination.
2 - 63
Page 76

2.5 Retry Code Table

Code Description Remarks
Allowable Retry Count
1 Alarm fuse blown 1/50 ms 2 12 V for lamp 1/4.5 sec Check is not made when lamp is turned
off (during initialization, water exchange
or sleep). 3 15 V for CPU rack 1/50 ms 4 -15 V for CPU rack 1/50 ms
14 Liquid in vacuum tank 1/4.5 sec 15 Vacuum pressure abnormal 1/4.5 sec Check is not made within 10 sec after
vacuum pump turns on.
16 Waste solution tank 1/4.5 sec 17 Incubation bath water inadequate 100/4.5 sec Checked in the following statuses;
standby, operation, sampling stop.
18 Distilled water short 1) 10/6 sec Water supply
2) 50/6 sec Warning
3) 150/6 sec STOP
19 Distilled water sensor abnormal 1/6 sec Check is not made during initialization,
water exchange or wake-up.
31 GPCNT1 10 Only at power-on 32 GPCNT2 10 Only at power-on 33 GPCNT3 10 Only at power-on 34 GPCNT4 10 Only at power-on 35 GPCNT5 10 Only at power-on 36 GPCNT6 10 Only at power-on 37 GPCNT7 10 Only at power-on 38 GPCNT8 10 Only at power-on 46 GMCNT1 10 Only at power-on 47 GMCNT2 10 Only at power-on 48 GMCNT3 10 Only at power-on 49 50 ADC controller 1 Only at power-on 71 Checksum error detected in operation
unit
72 Checksum error detected in analyzing
unit 102 103 104 105 106 107
1
1
2 - 64
Page 77

2.5.1 Logging Program List

No. Function Output Method Processing
1 Monitor printout Real time printout: Specify "print" on
the start condition menu screen. Printout is made when the specified values in all tests for one sample are calculated during operation.
Batch printout: Specify sample number on the data monitor screen.
2 Calibration result
printout
3 Remaining
reagent volume printout
4 Cell blank printout Specify "cell blank" on the
5 Reproducibility
check printout
6 Cumulative
mechanism information printout
7 Parameter printout Specify "parameter" on the
8 Program check
printout
9 ISE check printout Specify "ISE" check on the mechanism
10 Photometer check
printout
11 Alarm trace
printout (DAILY)
12 Alarm trace
printout (CUMULATIVE)
13 Original Abs.
printout
14 Communication
trace printout
15 Work sheet
printout
16 Barcode Reader
check
Printout is made when "calibration" is selected on the start condition menu screen and the result of calibration is output.
Specify "remaining reagent volume printout" on the start condition menu screen.
maintenance screen. Specify "reproducibility check" on the
maintenance screen. Specify "cumulative mechanism
information" on the mechanism check menu screen.
maintenance screen. Specify "program check" on the
mechanism check menu screen.
check menu screen. Specify "photometer check" on the
maintenance screen. Specify "alarm trace information" on
the mechanism check menu screen. Specify "alarm trace information" on
the mechanism check menu screen.
Printout is made when "original Abs." is specified on the system parameter screen and operation is executed.
Specify "communication trace printout" on the mechanism check menu screen.
Specify "work sheet printout" on the TS registration screen of routine analysis.
Specify "Barcode Reader" check on the mechanism check menu screen
Real time and batch
Real time
Batch
Batch
Batch
Batch
Batch
Batch
Real time
Batch
Batch
Batch
Real time
Batch
Batch
Real time
Printout Stop by Stop Key
O
(In batch mode alone)
×
×
O
O
×
O
× ×
O
(Note 1)
O
O
×
(Note 2)
O
O
×
NOTES: 1. The stop key is invalid during printout.
2. The stop key need be pressed to stop analysis.
(1) Initialization of Printer
2 - 65
Page 78
Effected when power supply is turned on or there is a printer alarm at start of printing one processing.
(2) Paper Feed
At end of printout At the end of each printout (in batch mode of No. 1 and in Nos. 3 to 15), paper is fed by 3 lines. (Paper is also fed when printout is stopped by stop key input.)
(3) Page Length
Page length is not determined.
(4) Printing Performance
4 lines/sec (80 characters/sec), 2.54 cm/6 lines, paper feed in pitches of 4.23 mm/line (1/6 inch)
(5) Printing System
Numerals are right-justified with space at leading positions unless there is a comment.
(6) Printout with No Blank Space
Channel number is not registered in keyed-in information.
Measured data is unavailable.
(7) For Leaving Blank Space
Overflow has occurred at the location where measured data is to be printed.
2 - 66
Page 79

2.6 Daily Alarm Trace

(1) Outline
The alarm and retry data from power-on to present time point are printed when "daily" in "alarm trace data" is specified on the mechanism check menu screen.
(2) Details of Printout
(a) Title
The title "Daily Alarm Trace," date and time are printed. The printing order of year, month and day depends on the date printout order specification.
(b) Time (24-hour base)
Time when alarm and retry data has been saved onto FD.
(c) Instrument status
Numerical value representing the instrument status. For details, refer to Table 2-2.
(d) Occurrence count
Indicates how many times the same alarm/retry data as that in a single cycle has occurred successively (in the number of cycles within 1 to 999).
(e) Identification character
'A' is printed out for alarm data, and 'R' for retry data. Printout is not made when there is no data in each case.
(f) Alarm data
The alarm data is printed out in the order of main alarm code (3-digit integer), sub alarm code (3-digit integer) and time of occurrence up to 10 kinds. If 10 kinds are exceeded, excess ones are not stored. However, they are saved as cumulative data. Time of occurrence: Time on the basis of operation cycle (000 to 179) when the alarm has occurred (in 100 msec).
(g) Retry data
The retry data is printed out in the order of retry code (3-digit integer), retry count (3­digit integer) and time of occurrence up to 10 kinds. If 10 kinds are exceeded, excess ones are not stored. However, they are saved as cumulative data.
(h) Key operation data
The key operation data is printed out in the order of key operation code (3-digit integer), key operation count (3-digit integer) and time of key operation (3-digit integer). Printout is made only once.
2 - 67
Page 80
Table 2-2 Instrument Status Code Table
Blank row is the unassigned one.
Code Instrument Status Code Instrument Status Code Instrument Status
1 Initialization 23 45 Incubation bath water
exchange
2 Standby
(including parameter check)
3 Preparation for operation 25 47 Probe position adjustment
4 Operation 26 48 Probe position adjustment
5 Sampling stop 27 49 Probe position adjustment
6 28 50 Probe position adjustment
7 29 T/M stop 51 Probe position adjustment
8 Stop 30 Emergency stop 52 Mechanism check 9 31 53 Barcode Reader check
10 32 54 ISE check (internal standard
11 33 Rinsing (cell) 55 12 34 Rinsing (ISE) 56 13 35 Rinsing (all) 57 14 36 58 15 37 Photometer check 59 16 38 ISE priming (IS) 60 17 39 61 18 40 62 Routine sampling stop
19 41 ISE priming (REF) 63 20 42 64 21 43 Cell blank 65 Mechanism resetting during
22 44 66
24 46 Resetting
(sample probe rotation)
(sample probe up/down)
(reagent probe rotation)
(reagent probe up/down)
(stirrer)
electromotive force measurement)
(restart unallowable)
operation
2 - 68
Page 81
(3) Printout System
(a) Data is printed out in the sequential order starting from the newest data. (b) When the alarm/retry data in a single cycle does not reach 10 kinds, it is printed out
closely with no blank line left.
(c) When there is no alarm data or retry data, its title alone is printed.
(4) Notes
(a) Printout can be stopped in steps of cycle by stop key input. (b) Up to 500-cycle data starting from the oldest one is saved onto FD. However, if the
same data has recurred in consecutive cycles, storage is made as one-cycle data.
(c) In case of FD error, processing is terminated with the title alone printed.

2.6.1 Cumulative Alarm Trace

(1) Outline
The alarm data and retry data saved in the FD are printed when "cumulative" in "alarm trace data" is specified on the mechanism check menu screen.
(2) Details of Printout
(a) Title
The title "Cumul. Alarm Trace," date and time are printed. The printing order of year, month and day depends on the date printout order specification.
(b) Final alarm occurrence date/time
The printing order of occurrence date depends on the date printout order specification. Time printout is fixed to 24-hour base.
(c) Identification character
'A' is printed out for alarm data, 'R' for retry data and 'K' for key operation. Printout is not made when there is no data in each case.
(d) Alarm data
The alarms, which have occurred in a day, are printed out in the order of main alarm code (3-digit integer), sub alarm code (3-digit integer) and occurrence count (3-digit integer) up to 20 kinds. (Occurrence count: The cumulative number of identical alarms which have occurred in a day)
(e) Retry data
The retries, which have occurred in a day, are printed out in the order of retry code (3-digit integer) and retry count (3-digit integer) up to 20 kinds.
2 - 69
Page 82
(f) Key operation data
The key operation data is printed out in the order of key operation code (3-digit integer), key operation count (3-digit integer) and time of key operation (3-digit integer). Printout is made up to 16 kinds.
(3) Printout System
(a) Data is printed out in the sequential order starting from the newest data. (b) When the alarm/retry data in a day does not reach 20 kinds, it is printed out closely
with no blank line left.
(c) When there is no alarm data or retry data, its title alone is printed.
(4) Notes
(a) Printout can be stopped in steps of day by stop key input. (b) The data obtained from power-on to power-off is taken as daily data and the data for
up to 256 days is saved onto FD starting from the newest data. In case of continuous energization, however, a day's data is automatically closed when the day changes and subsequent data is treated as for the following day.
(c) In case of FD error, processing is terminated with the title alone printed.
2 - 70
Page 83
Alarm Trace Printout (DAILY)
Instrument status Occurrence count
Time of occurrence
Retry count (or sub alarm code)
Retry code (or main alarm code)
Alarm Trace Printout (CUMULATIVE)
Main code
Occurrence count
Sub code
Retry circuit
Retry code
2 - 71
Page 84

2.6.2 Parameter Code List

2 - 72
Page 85
(1) Parameter Printout
Table 2-3 Contents of Printout
Numerical Input from Screen
0 Title
1 to 37 Photometry assay parameter
38 to 40 ISE parameter
99 8calculation tests
8 compensation tests Control positions (1 to 5) Serum indexes Printing order Printing format (when with card printer) Set test Carry-over evasion System parameter DIP switch data
Contents of Printout Without ISE With ISE Remarks
O O O O
×
O
(× for ISE- related tests)
( O: Printed ×: Not printed )
O O
(2) Program Check Printout
Program version No.
2 - 73
Page 86

2.6.3 Communication Trace

(1) Outline
The contents of communication between the analyzer and external system are printed according to the specification of "communication trace printout" on the mechanism check menu screen.
(2) Printing Items and Their Contents
Details are given in Table 2-4.
Table 2-4 Printing Items and Their Contents
Printing Item Contents
Title Title "Communication Trace," date and time are printed.
The printing order of year, month and day depends on the date printout order specification.
Time Communication start time Communication end time
(hour, minute, second) (hour, minute, second)
Communication mode Transmission
Reception
Error message Refer to Table 2-5. Example of communication text Refer to the system I/F specification.
AU HOST HOST AU
2 - 74
Page 87
(3) Printout System
In printout of
(a) Printout order
Sequential printout starting from the latest communication
(b) Presence/absence of error message and printout system
Details are given in Table 2-5.
Table 2-5 Presence/Absence of Error Message and Printout System
Error Message
None Both transmission
BCC SUM
PARITY FRAMING OVER RUN FRAME LENGTH CHAR1
CHAR2 CHAR3
CHAR4 TIME OUT Both transmission
NOTE: Control codes and corresponding character strings are listed in Table 2-8.
Communication Mode
and reception
HOST AU
HOST AU HOST AU HOST AU HOST AU HOST AU HOST AU
HOST AU HOST AU
HOST AU
and reception
Contents of Error
None (normal) Only communication
BCC error or checksum error
Parity error All data printed Framing error All data printed Overrun error All data printed Frame error All data printed Text length error All data printed Function character
error Sample data error All data printed Test selecting
information error Comment error All data printed Transmission or
reception time-out error
Printout System (communication text example)
function No. and sample data
All data printed
All data printed
All data printed
Only time point, communication mode and error message printed
communication text (example), an ASCII control code is converted into a character string and printed, and then line is fed and communication text is printed.
Remarks
(Note)
(4) Notes
(a) Printout is terminated by stop key input. Printout can be stopped after the contents of
trace in one cycle have been printed.
(b) A text is printed on two lines when it is longer than 20 characters.
2 - 75
Page 88
Table 2-6 Control Codes and Corresponding Character Strings Printed
Control Code (NEX) Character String Use in AU (Analyzer Unit)
00 01 02 03 04 05 06 07 08 09
0A 0B 0C 0D 0E
0F 10 11 12 13 14 15 16 17 18 19
1A 1B 1C 1D 1E
1F
NUL
SOM
STX ETX
EOT
ENQ
ACK
BEL
BS NT
LF
VT
FF
CR
S0
S1 DLE DC1 DC2 DC3 DC4
NAK SYN
ETB
CAN
EM SUB ESC
FS
GS
RS US
O O
O
O
2 - 76
Page 89
(5) Communication Trace Printout
5 0 5 0
Communication Trace
94/07/25 16:30
08:11:17 – 08:11:18 AU–>HOST :A 10110ABCDEFG
08:11:11 – 08:11:12 HOST–>AU :A 10110ABCDEFG
08:05:08 – 08:05:09 AU–>HOST :A 10210ABCDEFG
08:05:00 – 08:05:01 HOST–>AU ?
08:04:10 – 08:04:11 AU–>HOST :A 10210ABCDEFG
08:03:00 – 08:03:01 HOST–>AU CHAR. ERR :A 05105ABCDEFGHIJKL A0101010101010101010 10104
Communication start and end time points Communication mode and error message Communication text example
2 - 77
Page 90

2.6.4 Cumulative Instrument Operation List

1. Routine; Routine sample 3. Cont.; Control serum
2. Calib.; Calibration solution 4. STAT; Stat sample
Total number of tests
Number of analyzed samples
2 - 78
Page 91
2 - 79
Page 92

3. FLOW PATH DIAGRAMS

3.1 List of Solenoid Valves.............................................................................................3-1
3.2 Overall Piping Diagramm..........................................................................................3-2
3.3 Flow Rate at Each Location .....................................................................................3-3
3.4 WASH ...................................................................................................................3-4
3.4.1 Details of WASH Processing......................................................................3-5
3.5 Carry-over Evasion Function....................................................................................3-6
3 - 0
Page 93
3. FLOW PATH DIAGRAMS

3.1 List of Solenoid Valves

SV No.
SV 1 2-way A2-5617 Open 24 V DC CKD Water supply intake 713-0321 SV 2 2-way HB-11-X0185 Open 24 V DC CKD Sample syringe 713-1059 SV 3 2-way HB-11-X0185 Open 24 V DC CKD Reagent syringe 713-1059 SV 4 2-way HB-11-X0193 Open 24 V DC CKD Incubation bath water intake 713-1379
SV 5 2-way HB-11-X0193 Open 24 V DC CKD SV 6 2-way HB-11-X0193 Open 24 V DC CKD
SV 8 2-way HB-11-X0193 Open 24 V DC CKD
SV 9 2-way HB-11-X0193 Open 24 V DC CKD SV 10 2-way HB-11-X0193 Open 24 V DC CKD SV 11 3-way A2-5619 24 V DC CKD SV 12 2-way NTV-2-HN Open 24 V DC Takasago SV 13 2-way NTV-2-HN Open 24 V DC Takasago
SV 14 3-way NRV 24 V DC Takasago Vacuum tank (evacuation) 713-0332 SV 15 2-way A2-5618 Open 24 V DC CKD SV 16 2-way MTV-21-SM6M Open 24 V DC Takasago ISE syringe 713-0317
SV 17 2-way MTV-21-SM6M Open 24 V DC Takasago ISE sample discharge 713-0324 SV 18 2-way MTV-21-SM6M Open 24 V DC Takasago ISE and REF aspiration 713-0325
2-way/ 3-way
Type
Open/Close When Energized
Voltage Maker Application Part No.
Stirring and washing water supply
Reagent probe outside washing water supply
Sample probe outside washing water supply
Rinsing and nozzle tip washing water supply
Rinsing and cell blank water supply
Rinsing and cell washing water supply
Waste solution discharge (low concentration)
Waste solution discharge (high concentration)
Incubation bath water discharge
713-1379 713-1379
713-1379 713-1379 713-1379 707-0290 713-0331 713-0331
713-0320
SPV 1 Pinch
valve
SD10BA-2A-00T Open 24 V DC Advance ISE aspiration 707-0327
3 - 1
Page 94
SV4
SV10
SV9
SV6
SY2
SY3
SY16
SV5
SV8
3 - 2
Page 95

3.3 Flow Rate at Each Location

Location
S probe Outside
washing Inside
washing
R probe Outside
washing Inside
washing
Stirring and washing
Rinsing High
concentration waste solution probe
Low concentration waste solution probe
Cell blank washing
Nozzle tip washing
Incubation bath flow rate
Returning from water supply pump
Flow rate through lamp 0.30 or more
Flow Rate mL/min
270 ± 30
35 ± 5
270 ± 30
120 ± 20
320 ± 30
90 ± 10
90 ± 10
450 ±150
(µL/0.8 sec)
140 ± 15
3.7 ± 0.2 (L/min)
(L/min)
Remarks
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.0/tube L = 650 Tube = Tygon ø3.17 × ø6.35
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.0/tube L =820 Tube = Tygon ø3.17 × ø6.35
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.15/tube L = 1000 Tube = Tygon ø3.17 × ø6.35
SV11/branch tube/tube L = 395/high concentration waste solution probe
Tube = Tygon ø2.38 × ø3.96 Same as above
SV unit restrictor ø1.5/tube A = 580, specials/tube B = 500 Tube A = Tygon ø3.17 × ø6.35, tube B = Silastic tube ø1.0 × ø3.0
SV unit restrictor ø1.5/tube L = 1080 Tube = Tygon ø2.38 × ø3.96
After pouring water into the incubation bath with cells set, flow velocity on water surface should be measured with cells removed (in sec/circumference).
Measure with the lamp coolant tube disconnected at the lamp inlet.
Restrictor ø1.0
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Page 96

3.4 WASH

3.4.1 Details of WASH Processing

START
1)
Operator's
judgment
2)
Made
Abnormal
Unnecessary
Maintenance screen
WASH necessary?
Necessary
"Execution" input
WASH
"STOP" input
Operation check Normal
Function : Washing of each mechanism Necessary : WASH (cell) 15 min 20 sec
WASH (ISE) 5 min 44 sec WASH (All) 20 min 20 sec
3)
Alarm indication
4)
FD (alarm logging)
END
Step No. Contents of Processing Reference Document
1) Start from MAINTENANCE screen Screen specifications
2) See next page
3)
4)
When mechanism operates, its operation is checked. If abnormal, alarm is indicated.
Alarm will be saved into the alarm FD.
Screen specifications Alarm code table FD specifications
Remarks: (1) Start method and resulting action
(a) Specify DAILY.
All cells, probes (S and R), stirrer and ISE mechanism are washed. (b) Specify reaction cell. (c) Specify ISE.
The ISE mechanism is washed.
NOTE: Specification of ISE for WASH
when without ISE mechanism...............Input cannot be made from the screen.
Specification of all mechanisms
when without ISE mechanism...............Operation is carried out according to "cell"
specification.
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Page 97
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Page 98

3.5 Carry-over Evasion Function

(1) Outline
The carry-over evasion function is provided to prevent occurrence of inaccurate data due to sample carry-over in photometry assay and ISE tests. This function works on routine samples alone and does not work on calibrator and control samples. Carry-over is evaded by changing the measuring sequence among the reagents or samples, which may cause carry-over, or by execution of washing. The kinds of evasion/washing are listed below.
(a) Evasion of reagent carry-over due to reagent probe and washing for evasion (b) Evasion of carry-over between reagents due to cell and washing for evasion (c) Evasion of sample carry-over due to sample probe and washing for evasion
(a) Evasion of reagent carry-over due to reagent probe and washing for evasion
(I) Condition for carry-over evasion
When the evasion of reagent carry-over due to reagent probe is set, it is attempted at first by changing the reagent sampling sequence (test sequence). If this method is unusable, carry-over evasion by washing is executed. That is, when the sequence of pipetting reagents (R1 to R3) with the reagent probe matches the specified pattern, carry-over is prevented by (i) to (iii) below. In (ii) and (iii), throughput is degraded.
(i) For avoiding the relevant pattern, channel registration is changed for a
different test within the test sequence of the same sample.
(ii) When step (i) cannot be taken, a channel is registered after reagent probe
washing cycle.
(iii) Cell is made blank and the system waits till deviation from the specified
pattern.
(II) Method of carry-over evasion
The reagent probe is washed by aspirating detergent (detergent 1 or 2) or system water (distilled water for washing the inside of probe) and discharging it into a cell. At this time, the stirring rod is also washed in that cell.
(III) Carry-over evasion-specified pattern
The patterns, for which the evasion of carry-over between reagent types is specified, are listed below. " ALL" indicates that evasion is required in all types of R1, R2 and R3. ALL represents 3 reagent types for 3-reagent system, and 2 reagent types for 2­reagent system.
R3 R3, R3 R2, R3 R1, R2 R3, R2 R2, R2 R1, R1 R3, R1 R2, R1 R1
(IV) Registration of carry-over evasion-specified pattern
Up to 40 kinds of patterns, for which the evasion of carry-over due to the reagent probe is specified, can be registered.
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Page 99
(V) Probe washing cycle
350
µ
L)
Probe washing employs one (1) machine cycle. Within one machine cycle, the specified detergent (system water) placed on the reagent disk is aspirated in the specified volume and discharged into the cell at the same timing as for reagent aspiration and discharge.
(VI) Measure for ISE
Of the reagents for ISE, the internal standard solution and diluent are applicable to carry-over evasion. Carry-over evasion cannot be specified for reference electrode solution.
(VII) Specification from screen
From the screen, the following need be specified; a carry-over giving test and its reagent type (R1, R2, R3), a carry-over receiving test and its reagent type (R1, R2, R3), detergent set position on the reagent disk and detergent volume. An example of specification is shown below.
[GPT] [1] [LDH] [4] [38] [350]
Carry­over giving test (CH1 to 36)
Reagent type (1:R1 2:R2 3:R3)
Carry­over receiving test (CH1 to
Reagent type (1:R1 2:R2 3:R3)
36, ISE
Detergent set position (1 to 39...ISE excluded 1 to 36,39...ISE allowed
W1..........System
water)
ALL)
Detergent discharge volume (50 µL to
(VIII) Examples of evading procedure
Examples of carry-over evading procedure are shown below. A to C represent tests, and W stands for washing.
Example of Carry-over Evasion-specified Pattern
A A
A
R3
R3 R3
B
R2
B
R3
B
R1
A
R3
A
R3
A
R3
Example of Evading Procedure
C
(example of succeeding in evasion by
R2
change in channel registration) W B W B
R3 R1
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Page 100
(b) Evasion of carry-over between reagents due to cell and washing for evasion
(I) Condition for carry-over evasion
The cell used for analysis of the specified test is not used for analysis in the next round (after 48 cycles). Instead, it is washed for prevention of carry-over.
(II) Method of carry-over evasion
Cell is washed by aspirating detergent (detergent 1 or 2) or system water and discharging it into a cell. At this time, washing is facilitated by stirring. Carry-over evasion of cell is also effected during S. Stop. After completion of washing all cells, auto stop occurs. When stop status is set for any cause other than auto stop, cell washing for evading carry-over due to the previous operation will not be resumed in start from the standby status. Execution of cell washing degrades throughput.
(III) Registration of carry-over evasion-specified pattern
Up to 10 kinds of patterns, for which the evasion of carry-over due to cell is specified, can be registered.
(IV) Specification from screen
From the screen, the following need be specified; a carry-over giving test, detergent set position on the reagent disk and detergent volume. An example of specification is shown below.
[TG] [38] [350]
Carry­over giving test (CH1 to 36)
Detergent set position (1 to 39...ISE excluded 1 to 36,39...ISE allowed W1…System water)
Detergent discharge volume (50 µL to 350 µL)
(V) Cell washing cycle
When detergent discharge volume exceeds 350 µL, detergent is discharged by using 2 timings among the 3 timings (R1, R2, R3) for reagent aspiration and discharge in one cycle.
(VI) Example of evading procedure
An example of carry-over evading procedure is shown below. A to E represent tests, and W stands for washing.
Carry-over Evasion-specified Test
A
a-th cycle............ B A C.......
(a -1)th cycle....... D W E.......
Example of Evading Procedure
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