signal word indicates an immediate dangerous situation that
will result in death or serious
injury if it is not avoided.
WARNING!This combination of symbol and
signal word indicates a possible
dangerous situation that can
result in death or serious injury
if it is not avoided.
CAUTION!This combination of symbol and
signal word indicates a possible
dangerous situation that can
result in minor injury if it is not
avoided.
NOTICE!This combination of symbol and
signal word indicates a possible
dangerous situation that can
result in material and environmental damage if it is not
avoided.
arning categories
W
General symbols
Warning signsType of danger
Warning – hand injuries.
Warning – high-voltage.
Warning – danger zone.
This listing symbol denotes descriptions of tasks that you
must perform.
n This dot is for denoting lists.
–This dash denotes listings of the second level.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN5
Introduction
Cross reference within the document
Symbols on the device
Cross references are indicated as follows:
bols’ on page 5
Warnings/symbols on the device which are no
longer recognizable should be immediately
replaced by the operating company.
The images shown in the following depict the positions of the warnings and symbols affixed to the
device.
Ä
Chapter 1.1 ‘Sym-
Fig. 1: Warnings on the device
Attention, general danger point
Nonobservance of this warning can lead to material damage and
personal injury.
Before using the device, make sure you read the operating instructions and observe the safety information.
arning - Biological contamination!
W
Before using the device, make sure you read the operating instructions and observe the safety information.
Warning - Hand injury
Nonobservance of this warning can lead to material damage and
personal injury.
Before using the device, make sure you read the operating instructions and observe the safety information.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 6
Introduction
Fig. 2: Warning stickers on the right outer side
Attention, general danger point
Nonobservance of this warning can lead to material damage and
personal injury.
Before using the device, make sure you read the operating instructions and observe the safety information.
Symbol for separate collection of electric and electronic
devices
Symbol according to directive 2002/96/EC (WEEE). Applies in the
countries of the European Union, as well as in Norway and Switzerland.
T
ype plate
Nameplate with technical data specifications.
Symbol for TÜV-inspected device
From TÜV-inspected device.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN7
IOIOI
RS232
I
O
I
OI
RS23
2
IOIOI
RS232
Introduction
Fig. 3: Notice at the RS232 interface
Symbol for RS232 interface
This symbol indicates the RS232 interface (only for device with
RS232 interface).
1.2 Personal protective equipment
The assessment of the personal protective equipment must take
place on site according to the actual risks which are posed by the
used substances, the procedures and the environmental conditions.
The operating company must create a risk assessment according
to the corresponding standards and directives and enact work
instructions which ensure the safe handling of the device and
accessories.
1.3 Personnel qualification
Repairs may only be performed by a person authorized by the
manufacturer.
NOTICE!
Interventions and modifications to devices by persons not authorized by the company Andreas Hettich GmbH & Co. KG will be at one's own risk and
result in the voiding of all warranty claims, as well
as the voiding of liability claims against Andreas
Hettich GmbH & Co. KG.
Authorized skilled personnel with special instruction
Skilled personnel are able to perform the work assigned to them
and independently recognize and avoid potential hazards based on
their specialized education (professionally recognized vocational
training), knowledge and experience as well as knowledge of the
relevant regulations.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 8
Introduction
Personnel have participated in special instruction by the manufacturer of the device or personnel authorized by the manufacturer
and, through this, are authorized to perform the corresponding
task.
Personnel have completely read and understood this documentation.
Service technician
A service technician is someone who, based on his professional
training and advanced practical knowledge, provides expert support (installation, commissioning, maintenance, etc.) for our customers in all things mechanical, on the phone as well as on site,
and is an employee of the company of the manufacturer or was
trained and authorized by the manufacturer to carry out service
work on the device.
1.4 Intended use
Intended use
Improper use
Dual centrifugation: The purpose of the ZentriMix is to process
samples pre-analytically in non-medical laboratories.
This includes the extraction, grinding, mixing and dissolving as well
as homogenizing of samples, so also the decomposition of organic
or inorganic materials.
Another purpose is the creation of nanoparticles through homogenization (e.g. lipid nanoparticles, particularly liposomes) for nonclinical uses.
Classical centrifugation: In its function as a classical centrifuge, it
is used for the separation of materials or material mixtures with a
density of max. 1.2 kg/dm³ in non-medical laboratories.
The device was not designed for use in environmental conditions
that do not meet the manufacturer requirements or in a potentially
explosive, radioactive or biologically or chemically contaminated
atmosphere.
The ZentriMix is only meant for the above-named purposes.
Another use or one which goes beyond this is considered to be
improper. The manufacturer is not liable for damage resulting from
this.
Intended use also includes the observation of all information in the
operating instructions and complying with the inspection and maintenance intervals.
The device was not designed for use under environmental conditions that do not meet the manufacturer requirements or in a potentially explosive, radioactive or biologically or chemically contaminated atmosphere.
The device is not meant for processing toxic, corrosive or infectious samples; such samples must not be processed in the ZentriMix.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN9
Introduction
Foreseeable misuse
The device is built according to the state-of-the-art and the recognized safety regulations. If used and handled improperly, there
could be life-threatening danger to the user or third parties, or the
device could be impaired or there could be other property damage.
The device is only to be used for its intended purpose and only
when it is in safe working condition. Malfunctions which could
fect safety must be corrected immediately.
af
If consumable material approved/sold by the manufacturer is not
used, there is a danger of container damage at high speeds, which
could result in a spontaneously high unbalance and damage to the
device.
Do not operate the device unsupervised.
If used improperly, incorrect input of the parameters can result in
dangerous operating conditions: For example, if the duration of a
run is entered too high, the sample could overheat, which could
cause the sample container to burst, which could result in extreme
unbalance at high speed and damage to the device.
1.5 Replacement parts/consumable material
Only original replacement parts and approved original accessories
of the manufacturer may be used and only original consumable
material or that recommended (approved) by the manufacturer in
its official documentation may be used.
1.6 Scope of delivery
WARNING!
If original replacement parts, consumable material
approved or sold by the manufacturer and original
accessories are not used, any warranty or liability
claims against the company Andreas Hettich
GmbH & Co. KG are void.
n 1 x device with mounted rotor
n 1 x mains cable (power supply)
n 1 x size 5 Allen wrench (for emergency release and mounting/
dismounting the rotor)
n 1 x lubricating grease for lifting lugs
n 1 x operating instructions
Rotor(s) and the corresponding accessories are
included in delivery, depending on the order
.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 10
1.7 Return shipments
Introduction
If the device or its accessories are sent to the manufacturer, this/
these must be decontaminated and cleaned before shipping in
order to protect personnel, the environment and material. Please
contact the Service department of the manufacturer for the return
shipment. You will then receive a clearance certificate and return
material authorization number
ance certificate will not be accepted in order to protect our
employees. We ask for your understanding.
. Devices without a signed clear-
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN11
Introduction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 12
2 Safety information
Danger due to electrical energy
Safety information
The device and its associated components and
assemblies meet the currently valid safety standards individually and as a whole and meet the CE
directives of the European Union.
The device is safe if used as intended and
observing the descriptions and information given in
this documentation.
WARNING!
W
arning of incorrect voltage on the device pro-
tection switch!
Pay attention to the correct operating voltage in
order to prevent damage to the device.
Only operate the device at the correct oper-
–
ating voltage.
–Refer to the technical data and nameplate for
the value for the correct operating voltage.
–If the operating voltage is too high, the overcur-
rent protection fuse in the device switch can
trip and the device electronics can be
destroyed.
Danger due to kinetic energy
DANGER!
Danger due to rotor moving with high kinetic
energy!
There is a risk of injury if the lid is opened via the
emergency release during device operation.
–Only open the device via the emergency
release when the device is switched off.
Before the emergency release, make sure that
–
the device rotor has stopped.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN13
Safety information
Danger due to insufficient maintenance or maintenance not performed in a timely manner
DANGER!
Danger due to insufficient maintenance or
maintenance not performed in a timely manner!
There is a risk of collision inside the device due to
insufficient maintenance or maintenance not performed in a timely manner
–Have maintenance done at the specified inter-
vals.
–Check the rotor for a firm fit before using the
device. Correctly tighten the fastening screw. If
the rotor has play, it must be replaced.
–Check the device for visible damage or defi-
ciencies before use. If you discover deficiencies or damage, put the device out of operation
and immediately inform an authorized service
technician.
.
Warning of premature program
abort
Warning of hand injury due to rotor
gears
WARNING!
arning of premature program abort!
W
In the event of a premature program abort, such as
a power failure, switching of
run or pulling out the mains plug, the desired effect
on the samples might not be achieved.
–Do not switch off the device during a program
run.
–Do not actuate the emergency release during a
program run.
–Do not pull out the mains plug during a pro-
gram run.
–After a program abort, check whether the sam-
ples can be used.
CAUTION!
Warning of hand injury due to rotor gears!
There is a risk of injury to fingers by the rotor gears
if this is turning. Cuts with possible infections could
result.
Do not grab between the gears of the rotating
–
units and the rotor.
–Avoid rotor rotation with the lid open as much
as possible.
–To prevent injuries to third parties, do not leave
the device unsupervised with the lid open.
f during the program
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 14
Risk of injury due to the device
falling out of the packaging
Warning of hand injury due to
device lid falling closed unbraked
Safety information
WARNING!
Risk of injury due to parts falling out of the
packaging!
When parts fall out of the transport packaging,
there is a risk of crushing injuries to extremities.
–Observe the warnings for opening the pack-
aging.
–Only open the packaging at the specified point.
WARNING!
W
arning of hand injury due to device lid falling
closed unbraked!
If the lid falls closed unbraked, there is a risk of
extremities getting caught.
–Have the device maintained by a service tech-
nician at the specified intervals.
Always open the device lid completely.
–
–If a gas pressure spring of the lid is defective,
have it replaced by a service technician.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN15
Safety information
Danger posed by selection of
unsuitable parameters for dual centrifugation
WARNING!
Danger posed by selection of unsuitable
parameters for dual centrifugation!
The maximum permissible speed (RPM) for ZentriMix applications depends on the sample composition and sample container. Every ZentriMix
sample container can only be used up to a certain
rotational speed depending on the design and
application; this must not be exceeded under any
circumstances! Otherwise, there is a danger that
the sample container will not withstand the stress,
and the device will be damaged due to the
resulting unbalance; the heat that arises cannot be
compensated by the cooling system, resulting in
the sample container becoming very hot. Only
remove such a container after a suf
off time while wearing appropriate protective
clothing.
–ZentriMix applications are to be carried out
according to the protocol authorized by the
manufacturer.
–Every application not yet described by a manu-
facturer protocol must be checked and validated for any hazard potential before its use.
Ä
Further information on page 41.
–The correct parameter input must always be
checked.
–Do not leave the device unsupervised.
ficient cooling-
Damage due to incorrect loading
for dual centrifugation
WARNING!
Damage due to incorrect loading for dual cen-
trifugation!
The two rotor receptacles must be loaded strictly
symmetrically with the same weight.
–Observe the loading rules. Ä Chapter 6.5.2
‘Loading a rotor for dual centrifugation’
on page 37!
–Observe the device as it starts up. If unusually
high unbalance occurs, stop the run immediately by pressing the [Stop/Open] key twice
and check the load. If you cannot determine
that the load is incorrect, put the device out of
operation and inform the Service department.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 16
Danger due to hazardous substances in the sample
Safety information
DANGER!
Danger due to hazardous substances in the
sample
No materials or material mixtures may be processed in a way which poses a risk of fire or explosion.
–Therefore, thoroughly check the intended
process regarding this hazard and take the
appropriate measures to avoid personal injury.
–
Follow the relevant regulations and directives
for handling chemicals and hazardous substances.
–In particular, no aggressive chemicals may be
used (e.g. dangerous, corrosive extracting
agents, such as chloroform, strong acids).
Explosion and fire hazard
WARNING!
Explosion and fire hazard
Do not process any explosive materials or materials which can react together chemically with high
. Substances which could create a haz-
energy
ardous explosive or flammable atmosphere in the
device may not be processed. The estimation of
such risks is the responsibility of the operating
company
user with regard to this.
–Therefore, thoroughly check the intended
–Follow the relevant regulations and directives
–In particular, no aggressive chemicals may be
. The operating company must instruct the
process regarding these hazards and take the
appropriate measures to prevent personal
injury.
for handling chemicals and hazardous substances.
used (e.g. dangerous, corrosive extracting
agents, such as chloroform, strong acids).
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN17
Safety information
Danger due to the penetration of
water or other liquids
Warning - Impermissible ambient
temperature
DANGER!
Danger due to the penetration of water or other
liquids!
When water penetrates into the device, there is a
danger of a short circuit and the user suffering an
electric shock.
–
Prevent water and moisture from penetrating
into the device.
–Do not pour liquids into the device interior.
–The device must not stand in the rain.
–Transport is only allowed with suitable trans-
port protection.
WARNING!
arning - Impermissible ambient temperature!
W
If the ambient temperature is outside of the permissible ambient temperature range for the samples,
these might be compromised.
–
Observe the permissible maximum and min-
imum ambient temperatures for setting up the
device.
–Do not place the device next to heat sources or
devices which radiate heat.
–Check the samples after any device over-
heating to see if they can still be used and take
new samples, if necessary.
–Make sure the device is not exposed to direct
sunlight.
–Make sure the device is not exposed to frost.
–Maintain the minimum clearance around the
device.
–Make sure that the fan is not blocked.
–Never operate the device in a continuous run.
Warning - Position changed due to
vibration
WARNING!
Warning - Position changed due to vibration!
The device might fall down if the set-up surface
vibrates or if it is not flat or stable enough.
Only place the device on a stable, flat surface.
–
–The set-up surface must reliably bear the
weight of the device.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 18
Mechanical emergency release
Safety information
WARNING!
There is a risk of injury if the mechanical emer-
gency release of the device is actuated during
the program run.
If the mechanical emergency release of the lid is
actuated during a program run, the program is
aborted and the rotor runs down unbraked.
–Only actuate the emergency release when the
rotor is stopped.
–Check whether the rotor has stopped before an
emergency release.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN19
Safety information
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 20
3 Device description
1
2
1
2
12
Fig. 4: Operating elements, front side
Device description
1Control panel
2Lid
Further information about the control panel can be
found in the Operation chapter Ä Chapter 6 ‘Operation’ on page 31.
If the ambient temperature is outside of the permissible ambient temperature range for the samples,
these might be compromised.
–
Observe the permissible maximum and min-
imum ambient temperatures for setting up the
device.
–Do not place the device next to heat sources or
devices which radiate heat.
–Check the samples after any device over-
heating to see if they can still be used and take
new samples, if necessary.
–Make sure the device is not exposed to direct
sunlight.
–Make sure the device is not exposed to frost.
–Maintain the minimum clearance around the
device.
Transport
n The device can be stored in the original packaging.
n The device may only be stored in dry rooms.
n The storage temperature must be between - 20 °C and + 60
°C.
n The humidity must be non-condensing and must be between
20 % and 80 %.
n Observe the weight of the device during transport.
n When transporting with a transport aid (e.g. transport cart), this
must be able to bear a weight at least 1.6 times the permissible
transport weight.
n Secure the device against tipping over and falling down during
transport.
n Never transport the device on its side or upside-down.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 24
5 Commissioning
Commissioning
n Set up and level the device at a suitable location so that it is
stable. During setup, the required safety zone of textvar object
does not exist around the device must be complied with in acc.
with textvar object does not exist.
WARNING!
During a centrifugation run, no personnel, hazardous substances or objects may be within a
safety zone of textvar object does not exist around
the device in acc. with textvar object does not
exist.
n Ventilation openings may not be blocked. A distance of textvar
object does not exist to the air slots and ventilation openings
must be complied with.
n Before commissioning, check to make sure the mains voltage
is correct according to the nameplate and that the mains cable
is connected properly
n Only trained and instructed personnel may commission and
use the device.
Every time before the device is used, make sure that
.
Unpacking the device
5.1
n the device is dry (no moist residues from cleaning agents, etc.).
n no objects are lying in the centrifuge chamber under the rotor.
n the rotor position corresponds to the home position.
n the accessories are correctly connected to each other (leak-
proof) and the rotor is correctly inserted.
n the shown displays are correct.
Personnel:
The device is packaged in a box.
When lifting up the device, note the specified
weight and only lift the device with a suitable
number of helpers to avoid injuries.
Authorized skilled personnel with special
n
instruction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN25
Commissioning
WARNING!
Warning - Position changed due to vibration!
The device might fall down if the set-up surface
vibrates or if it is not flat or stable enough.
–
Only place the device on a stable, flat surface.
–The set-up surface must reliably bear the
weight of the device.
WARNING!
Risk of injury due to parts falling out of the
packaging!
When parts fall out of the transport packaging,
there is a risk of crushing injuries to extremities.
–Observe the warnings for opening the pack-
aging.
Only open the packaging at the specified point.
–
1.Remove the packaging bands.
2.Lift up the box and remove the padding.
3.Remove the accessories.
4.Set up the device on a stable and flat surface with a suitable
number of helpers. Note the weight of the device.
NOTICE!
Check the device for any transport damage before
commissioning. Do not put the device into operation if there is transport damage or if moisture has
penetrated it.
5.2 Setting up, connecting and switching on the device
Setting up the centrifuge
Switching off the device with the device switch
does not disconnect this completely from the
mains. The device is completely disconnected from
the mains by pulling out the mains cable. The
mains cable must be freely accessible at all times
in order to be able to disconnect the device from
the mains at the power socket or device plug.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 26
Commissioning
Personnel:
Authorized skilled personnel with special
n
instruction
When lifting up the device, note the specified
weight and only lift the device with a suitable
number of helpers to avoid injuries.
WARNING!
Warning - Position changed due to vibration
The device might fall down if the set-up surface
vibrates or if it is not flat or stable enough.
–
Only place the device on a stable, flat surface.
–The set-up surface must reliably bear the
weight of the device.
DANGER!
Danger due to the penetration of water or other
liquids
When water penetrates into the device, there is a
danger of a short circuit and the user suffering an
electric shock.
Prevent water and moisture from penetrating
–
into the device.
–Do not pour liquids into the device interior.
–The device must not stand in the rain.
–Transport is only allowed with suitable trans-
If the ambient temperature is outside of the permissible ambient temperature range for the samples,
these might be compromised.
–
Observe the permissible maximum and minimum ambient temperatures for setting up the
device.
–Do not place the device next to heat sources or
devices which radiate heat.
–Check the samples after any device over-
heating to see if they can still be used and take
new samples, if necessary.
–Make sure the device is not exposed to direct
sunlight.
–Make sure the device is not exposed to frost.
–Maintain the minimum clearance around the
device.
–Make sure that the fan is not blocked.
–Never operate the device in a continuous run.
Connecting the device
Fig. 9: Power supply connection
1Mains cable connection
Set up the device on a stable and flat surface with a suitable
number of helpers. Note the weight of the device.
Personnel:
1.
Make sure the operating voltage is correct,
as specified on the nameplate.
Connect the mains cable included in delivery to the device
plug on the device.
2.Stick the other end in the socket.
Authorized skilled personnel with special
n
instruction
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 28
Switching on the device
1
1 20 9/;\9 1500 2:00
PROG T/°C
RCF
RPM
t/min
Commissioning
Fig. 10: Device switch
1Device switch [ON/OFF]
Fig. 11: Start screen
Personnel:
Authorized skilled personnel with special
n
instruction
1.Switch the device on.
The control will start up. On the display, the device type,
ð
the software version and (depending on the setting) the
last loaded program or program place no. 1 are shown.
2.
Open the lid
Ä
Chapter 6.2 ‘Opening the lid’ on page 35.
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN29
1
N > ROTOR MAX
PROG T/°C
RCF
RPM
t/min
Commissioning
Fig. 12: Transport securing device
1Transport securing device
3.
NOTICE!
Before commissioning the device, remove
the transport securing device from the interior.
Remove the transport securing device.
NOTICE!
Check to make sure the rotor is mounted correctly before commissioning.
4.The device is now ready for operation.
If ‘Enter max cycles = <30000>’ is displayed after
switching on the device, the maximum permissible
number of running cycles (specified on the rotor)
must first be entered before the centrifugation run
can be restarted.
If the speed in the activated program is higher than
the maximum rotor speed (Nmax), no centrifugation run can be started. ‘N > ROT
Ä
played
Chapter 9 ‘Troubleshooting’
on page 89.
OR MAX’ is dis-
ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN 30
Loading...
+ 79 hidden pages
You need points to download manuals.
1 point = 1 manual.
You can buy points or you can get point for every manual you upload.