Hanna Instruments pH 209V User Manual

Instruction Manual

pH 209V

Complete Laboratory Kit

for pH & ORP Measurements

in Wine, Must and Grape Juice

www.hannainst.com

PRELIMINARY EXAMINATION

Dear Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument. This

manual will provide you with all the necessary information for the correct use of
the instrument, as well as a precise idea of its versatility in a wide range of
applications.

If you need additional technical information, do not hesitate to e-mail us at
[e-mail].

This instrument is in compliance with the directives.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION .....................................................................3

GENERAL DESCRIPTION ............................................................................. 3

FUNCTIONAL DESCRIPTION .......................................................................4

SPECIFICATIONS........................................................................................ 5

OPERATIONAL GUIDE ................................................................................ 6

pH CALIBRATION ......................................................................................8

pH VALUES AT VARIOUS TEMPERATURES ................................................ 10

pH ELECTRODE MAINTENANCE ................................................................. 11

ACCESSORIES .......................................................................................... 15

WARRANTY ............................................................................................. 22

Remove the kit from the packing material and examine it carefully to make sure
that no damage has occurred during shipping. If there is any damage, notify
your dealer.

The kit includes:

• pH 209 bench pH meter

• HI 1048B pH electrode

• HI 98501 electronic thermometer

• pH 3 and pH 7 buffer solutions (20 mL sachet each)

• HI 700635 and HI 700636 specific cleaning solutions (20 mL each)

• HI 7082 electrode refilling solution (30 mL bottle) and syringe

• HI 76404 electrode holder

• 12 Vdc adapter and instruction manual.

Note: Save all packing material until you are sure that the instrument

functions correctly. All defective items must be returned in the original
packing with the supplied accessories.

GENERAL DESCRIPTION

pH 209V is a complete laboratory kit for pH and ORP measurements in wine,
must and grape juice.

The kit also includes a special pH electrode (HI 1048B), which has been
designed for application in must and wine analysis, and a digital thermometer
(HI 98501) for measuring temperature and for using as reference during
manual temperature compensation procedure of pH readings.

The HI 1048B is a refillable pH electrode provided with a porous PTFE junction
sleeve, which ensures the right electrolyte flux, avoids clogging and allows
accurate pH measurements in “difficult” samples as must and wine.

This meter can be calibrated at 2 points, pH 7 and pH 3, and it is provided
with an easy manual temperature compensation.

The adjustable knobs are located on the front panel for easy operations.

32

FUNCTIONAL DESCRIPTION

SPECIFICATIONS

Range 0.00 to 14.00 pH / ±1999 mV
Resolution 0.01 pH / 1 mV
Accuracy (@20°C) ±0.01 pH / ±1 mV
Typical EMC Deviation ±0.03 pH / ±2 mV
pH Calibration Manual, 2 point, through offset and slope knobs
Temperature Compensation

Manual, from 0 to 100°C (32 to 212°F)

pH Electrode (included)

HI 1048B glass body, double junction,

refillable, with BNC and 1 m (3.3') cable

Input Impedance 1012 ohm
Power Supply 12 Vdc adapter (included)
Environment 0 to 50°C (32 to 122°F);

RH max 95% non-condensing

Dimensions 240 x 182 x 74 mm (9.4x7.2x2.9")
Weight 1.0 kg (2.2 lb.) - meter only

1) Liquid Crystal Display (LCD)

2) pH slope adjustment knob

3) pH offset adjustment knob

4) pH range selection key

5) mV range selection key

6) °C range selection key

7) Temperature setting knob

8) ON/OFF switch

9) Power adapter socket

10) BNC connector for pH (or ORP) electrode

11) Electrode reference socket

54

OPERATIONAL GUIDE

Power connection

Plug the 12 Vdc adapter into the power supply socket (#9 on page 4).

Note: Make sure the main line is protected by a fuse.

Electrode connection

For pH or ORP electrodes with internal reference, connect the electrode to the
BNC socket on the rear panel (#10 on page 4).

For electrodes with separate reference, plug the BNC connector of the electrode
to the BNC socket (#10 on page 4), and the reference electrode to the proper
socket (#11 on page 4) on the rear panel.

Note: To prevent damage to the electrode, remove the pH electrode from the

solution before turning the meter off. With the meter OFF, detach the
electrode and immerse it in the storage solution.

TAKING pH MEASUREMENTS

Make sure that the instrument has been calibrated for pH before taking pH
measurements.

• Switch the instrument on by pressing the ON/OFF
button.

• Immerse the electrode tip (4 cm/1½") into the
sample and shake briefly.

(1½")

• Press the pH key to display the pH measurement.

• The display shows the pH value of the test solution compensated for
temperature.

If measurements are taken in different samples successively, it is recommended
to rinse thoroughly the electrode with water, and then for some of the next
solution to be measured.

TAKING ORP MEASUREMENTS

pH 209 can also measure ORP, using an ORP electrode. Hanna Instruments
offers a variety of ORP electrodes for this purpose. Contact your dealer for more
information.

• Connect the ORP electrode to the meter and sub­merge the tip (4 cm / 1½") into the sample to be
tested.

Note: ORP measurements are not compensated for temperature variation.

• Press the mV key to enter the mV mode and allow a few minutes for the reading
to stabilize.

• The display will show the ORP (mV) value.

4 cm

(1½")

4 cm

• Take the temperature of the solution with the sup­plied HI 98501 thermometer (e.g. 25°C).

• Press the °C key to display temperature setting on the LCD and adjust the
temperature knob to display the temperature of the sample.

AFTER MEASUREMENTS

• Press the ON/OFF button to switch the instrument off.

76

pH CALIBRATION

IMPORTANT

For better accuracy it is recommended to calibrate the instrument frequently.
Calibration should be performed whenever the pH electrode is replaced, after
performing a cleaning procedure or changing the reference electrolyte, and at
least once a month.

INITIAL PREPARATION

Pour small quantities of pH 7.01 (HI 7007) and pH 3.00 (HI 5003) solutions
into clean beakers.

RINSE

CALIBRATION

For accurate calibration, use two beakers for
each buffer solution; the first for rinsing the

HI 7007

HI 7007

electrode, the second for calibration.
Note: The electrode should be submerged at least 4 cm (1½") into the solution.

PROCEDURE

Note: If you need to calibrate to NBS standards, use pH 6.86 (HI 7006)

solution instead of pH 7.01.

Use the supplied HI 98501 thermometer as reference.

• Switch the instrument on by pressing the ON/OFF
button.

• Rinse and immerse the pH electrode in pH 7.01 buffer
and shake briefly. Wait a couple of minutes for the
reading to stabilize.

(1½")

• Press the pH key to select pH measurement.

• Wait a couple of minutes, then adjust the OFFSET knob until the display
shows the pH value at the noted temperature (see the "pH versus
temperature" chart at page 10).

• Rinse and immerse the pH electrode in pH 3.00 buffer and shake briefly.

• Wait a couple of minutes, then adjust the SLOPE knob until the display
shows the pH value at the noted temperature (see the "pH versus
temperature" chart at page 10).

• The pH calibration is now complete.

4 cm

• Note the temperature of the buffer solution using the
supplied HI 98501 thermometer (e.g. 20°C).

• Press the °C key to select temperature setting, then adjust the Temperature
knob until the LCD shows the noted temperature.

98

pH VALUES AT VARIOUS TEMPERATURES

pH ELECTRODE MAINTENANCE

pH readings are strongly affected by temperature. The calibration buffer
solutions are affected by temperature changes to a lesser degree than normal
solutions.

For manual temperature calibration please refer to the following chart.

TEMP pH VALUES

°C °F 3.00 6.86 7.01

0

32

3.07

6.98

7.13

5

41

3.05

6.95

7.10

10

50

3.03

6.92

7.07

15

59

3.02

6.90

7.04

20

68

3.00

6.88

7.03

25

77

3.00

6.86

7.01

30

86

3.00

6.85

7.00

35

95

3.00

6.84

6.99

40

104

2.99

6.84

6.98

45

113

2.99

6.83

6.98

50

122

2.99

6.83

6.98

55

131

2.99

6.84

6.98

60

140

2.99

6.84

6.98

65

149

2.99

6.85

6.99

70

158

3.00

6.85

6.99

Note: To prevent damage to the electrode, remove the pH electrode from the

sample before turning the meter off. With the meter OFF, detach the
electrode from the meter and immerse it in the storage solution.

Preparation

Remove the electrode protective cap. Do not be alarmed if any salt deposits are
present: this is normal with electrodes and they will disappear when rinsed with
water.
During transport tiny bubbles of air may have formed inside the glass bulb. The
electrode cannot function properly under these conditions. These bubbles can be
removed by "shaking down" the electrode as you would do with a glass
thermometer.

Sensitive bulb

To avoid clogging problems and ensure a fast response, the bulb must be kept
moist at any time. Store the electrode with few drops of HI 70300 storage
solution in the protective cap. NEVER USE DISTILLED OR DEIONIZED WATER FOR
STORING PURPOSES.

Periodic maintenance

Inspect electrode and cable. The cable used for the connection to the meter must
be intact and there must be no points of broken insulation. The connector must
be perfectly clean and dry. If any scratches or cracks are present, replace the
electrode.

Refilling the electrode

ALWAYS VERIFY THE ELECTROLYTE LEVEL.
If the electrolyte level is more than 2 cm below
the refilling hole, unscrew and remove the refill­ing hole cap.
Add the 3.5M KCl electrolyte solution (HI 7082)
by using the supplied syringe. Tighten back the
cap. Wait at least 1 hour before using the
electrode.

1110

Cleaning procedure
After use, clean the electrode by immersing it in the proper cleaning solution (HI

700635 or HI 700636) for 5 minutes.

If case of slow and unreliable readings, it is recommended to perform the
following procedure for cleaning the junction:

1. Shift upwards the PTFE collar. If the collar does not shift easily, immerse it

into warm water.

2. Wipe gently the electrode surface with a soft and clean tissue.

3. In case of persistent wine or must residual, wet the tissue with cleaning

solution (HI 700635 or HI 700636).

4. Put back the collar in its original position.

ACCESSORIES

pH CALIBRATION SOLUTIONS
HI 50003-01 pH 3.00 buffer solution, 20 mL sachet, 10 pcs
HI 50003-02 pH 3.00 buffer solution, 20 mL sachet, 25 pcs
HI 70007P pH 7.01 buffer solution, 20 mL sachet, 25 pcs
HI 5003 pH 3.00 buffer solution, 500 mL bottle
HI 7006L pH 6.86 buffer solution, 500 mL bottle
HI 7007L pH 7.01 buffer solution, 500 mL bottle
HI 8006L pH 6.86 buffer solution in FDA bottle, 500 mL
HI 8007L pH 7.01 buffer solution in FDA bottle, 500 mL

ELECTRODE STORAGE SOLUTION
HI 70300L Storage solution, 500 mL bottle
HI 80300L Storage solution in FDA bottle, 500 mL

Troubleshooting

Evaluate your electrode performance based on the following.

• Noise (Readings fluctuate up and down) could be due to:

– Clogged/Dirty Junction: refer to the cleaning procedure above.
– Loss of shielding due to low electrolyte level (in refillable electrodes

only): refill with fresh HI 7082 solution.

• Dry Membrane/Junction: soak in HI 70300 storage solution for 1 hour.

• Drifting: soak the electrode tip in warm (approx. 50-60°C) HI 7082
solution for one hour and rinse the tip with distilled water. Refill with fresh
HI 7082 solution.

• Low Slope: refer to the cleaning procedure above.

• No Slope: check the electrode for cracks in glass stem or bulb and replace
the electrode.

• Slow Response/Excessive Drift: soak the tip in HI 7061 solution for 30
minutes, rinse thoroughly in distilled water and then follow the cleaning
procedure above.

ELECTRODE CLEANING SOLUTIONS
HI 70000P Electrode rinsing solution, 20 mL sachet, 25 pcs
HI 7061L General cleaning solution, 500 mL bottle
HI 70635L Cleaning solution for wine deposits, 500 mL bottle
HI 70636L Cleaning solution for wine stains, 500 mL bottle
HI 7073L Protein cleaning solution, 500 mL bottle
HI 7074L Inorganic cleaning solution, 500 mL bottle
HI 7077L Oil & Fat cleaning solution, 500 mL bottle
HI 8061L General cleaning solution in FDA bottle, 500 mL
HI 8073L Protein cleaning solution in FDA bottle, 500 mL
HI 8077L Oil & Fat Cleaning solution in FDA bottle, 500 mL

ELECTROLYTE SOLUTION
HI 7082 3.5M KCl electrolyte solution, 4 x 30 mL, for double junction

electrodes

HI 8082 3.5M KCl electrolyte solution in FDA bottle, 4 x 30 mL, for

double junction electrodes

ORP PRETREATMENT SOLUTIONS
HI 7091L Reducing pretreatment solution, 500 mL bottle
HI 7092L Oxidizing pretreatment solution, 500 mL bottle

1312

ELECTRODES
HI 1048B Glass body, double junction, refillable pH electrode with

BNC connector and 1 m (3.3') cable, for wine, must and
grape juice

FC 100B PVDF body, double junction, refillable, pH electrode with

BNC connector and 1 m (3.3') cable, for food industry

FC 200B PVDF body, open junction, conic tip, Viscolene, non-refillable

pH electrode with BNC connector and 1 m (3.3') cable, for
meat & cheese

FC 210B Glass body, double junction, conic tip, Viscolene, non-

refillable pH electrode with BNC connector and 1 m (3.3')
cable, for milk and yogurt

HI 3131B Glass body, refillable, platinum ORP electrode
HI 3230B Plastic body, gel-filled, platinum ORP electrode
HI 4430B Plastic body, gel-filled, gold ORP electrode

OTHER ACCESSORIES
HI 710005 115 Vac/12 Vdc power adapter, US plug
HI 710006 230 Vac/12 Vdc power adapter, European plug
HI 76405 Electrode holder

WARRANTY

All Hanna Instruments meters are guaranteed for two years against defects
in workmanship and materials when used for their intended purpose and
maintained according to instructions.

Electrodes and probes are guaranteed for a period of six months.

This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed maintenance

are not covered.
If service is required, contact the dealer from whom you purchased the

instrument. If under warranty, report the model number, date of purchase,
serial number and the nature of the failure. If the repair is not covered by the
warranty, you will be notified of the charges incurred. If the instrument is to be
returned to Hanna Instruments, first obtain a Returned Goods Authorization
number from the Customer Service department and then send it with shipping
costs prepaid. When shipping any instrument, make sure it is properly
packaged for complete protection.

All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner.

HI 8427 pH and ORP electrode simulator with 1 m (3.3') coaxial

cable ending in female BNC connectors

HI 931001 pH and ORP electrode simulator with LCD and 1 m (3.3')

coaxial cable ending in female BNC connectors

HI 98501 Pocket-size thermometer (range -50.0 to 150.0°C)

Hanna Instruments reserves the right to modify the design, construction and

appearance of its products without advance notice.

Recommendations for Users

Before using this product, make sure that it is entirely suitable for the environment in which it is used.
Operation of this instrument in residential area could cause unacceptable interferences to radio and TV equipment, requiring the

operator to take all necessary steps to correct interferences.
The glass bulb at the end of the electrode is sensitive to electrostatic discharges. Avoid touching this glass bulb at all times.

During calibration of instruments, ESD wrist straps should be worn to avoid possible damage to the electrode by electrostatic
discharge.

To maintain the EMC performance of this equipment, the recommended cables noted in the user's manual must be used.
Any variation introduced by the user to the supplied equipment may degrade the instrument's EMC performance.

To avoid electrical shock, do not use this instrument when voltages at the measurement surface exceed 24 Vac or 60 Vdc.
To avoid damage or burns, do not perform any measurement in microwave ovens.

1514

SALES AND TECHNICAL SERVICE CONTACTS

Australia:

Tel. [phone] • Fax [phone]

China:

Tel. [phone] • Fax [phone]

Egypt:

Tel. & Fax [phone]

Germany:

Tel. [phone] • Fax [phone]

Greece:

Tel. [phone] • Fax [phone]

Indonesia:

Tel. [phone] • Fax [phone]

Japan:

Tel. [phone] • Fax [phone]

Korea:

Tel. [phone] • Fax [phone]

Malaysia:

Tel. [phone] • Fax [phone]

Singapore:

Tel. [phone] • Fax [phone]

South Africa:

Tel. [phone] • Fax [phone]

Taiwan:

Tel. [phone] • Fax [phone]

Thailand:

Tel. [phone] • Fax [phone]

United Kingdom:

Tel. [phone] • Fax [phone]

USA:

Tel. [phone] • Fax [phone]

For e-mail contacts and complete list of Sales and Technical
offices, please see www.hannainst.com

MANPH209V 07/05

16