If pH 4.01 buffer
solution is used
as the first point
the value of the
buffer is recognized
and displayed
with the blinking
stability indicator.
If pH 7.01 buffer solution is used as
the first point the buffer is recognized
with the blinking stability indicator.
When the reading is stable, the
stability indicator will disappear
and pH 7.01 will be calibrated.
If pH 7.01 is the only calibration point,
finish one-point procedure at right.
If using pH 4.01 as a second point,
continue two-point procedure at right.
For one-p oint calibrati on using pH 4.01 buff er go to proced ure B
For one or t wo-point calibr ation using pH 7.01 buffe r go to procedure A
A
B
When the
reading is s table,
the stab ility
indicator will
disappe ar.“Sto”
will be disp layed
when the
calibration
is saved.
Meter wil l
exit to
measurement
mode and th e
calibration
tag will be
displayed.
Meter will exit
to measurement
mode and the
calibration
tag will be
displayed.
Two- Point
Use pH 4.01 to
perform a twopoint calibration.
The value is
automatically
recognized and
displayed with the
blinking stability
indicator.
When the reading
is stable, the
stability indicator
will disappear.
“Sto” will be
displayed when
the calibration
is saved.
Meter will exit
to measurement
mode and the
calibration
tags will be
displayed.
Thank You
Thank you f or choosing a Han na Instrumen ts product. P lease read th is instruct ion
manual care fully before u sing this instru ment.
For techni cal suppor t, contact your local Hanna Inst ruments O ffice or email us
at tech@hannainst.com.
To find your lo cal Hanna Ins trument s Off ice or for a dditiona l informa tion on
Hanna Instruments products, visit www.hannainst.com
Meter Calibration
From measurement
mode, press and
hold the ON/OFF
button until “CAL”
is displayed.
When “7.01” blinks
on the display, place
the tip of the probe
into a pH 7.01 or
4.01 buffer solution.
One-Point
One-Point Calibration with pH 4.01
B
A
One or Two-Point Calibration with pH 7.01
pH 4.01 will then
blink on the display.
Ignore it an d press
ON/OFF button.
Operation
Press the ON/OFF button to turn the meter on. All tags
will be displayed.
The meter will go into measurement mode: current
reading and calibrated buffers are displayed.
Preliminary Examination
Remove the m eter from th e packing mat erial and examin e it carefully t o make sure that
no damage has o ccurred durin g shipment. If not iceable damag e is evident, con tact your
local Hanna In struments O ffice.
Each meter i s supplied with:
• pH 4.01 buffer s olution liquid sa chet (2 pcs.)
• pH 7.01 buffer solut ion liquid sachet (2 p cs.)
• Cleaning so lution for milk dep osits ( 2 pcs.)
• Electrode storage solution, 13 mL dropper
• Instruction manual
• Quality certificate
Note: Save all p acking material u ntil you are sure that t he instrument fun ctions
correctl y. All defective item s must be returned i n the original pa ckaging with the
supplied accessories.
Meter Overview
Preparation:
The pH elec trode is s hipped wi th a protec tive cap containin g storage solutio n. Before
using the meter, remove the protective cap and condition t he elect rode by soaking the
tip (bot tom 4 cm (1.5”)) in pH 7.01 buffer solutio n for seve ral minute s. Then follow the
calibration procedure.
• Do not be alar med if white cry stals appear aro und the cap. This is nor mal with pH
electr odes and they dis solve when rin sed with wate r.
• Turn the mete r on by pressing ON /OFF button.
• Remove the p rotect ive cap and i mmerse t he tip of th e electr ode in the s ample
to be tested.
NEVER IMMER SE THE ELECTROD E OVER THE MAXIM UM IMMERSION LEV EL.
• Stir gentl y and wait for a stab le reading.
• For best results, recalibrate periodically.
• After u se, rinse the elec trode with water an d store it with a few dro ps of storage
solutio n in the protect ive cap.
• Reatta ch the protect ive cap after e ach use.
DO NOT USE DIST ILLED OR DEIONIZED WATER F OR STORAGE PUR POSES.
Intended use
The measu rement of pH in milk is imp ortant in test ing for impuritie s, spoilage, and s igns
of mastit is infection. Whi le there are a number of fa ctors that af fect the composi tion of
milk, pH me asuremen ts can help p roducer s unders tand wha t might be caus ing cert ain
composi tional changes. pH me asurement is common ly performed at va rious points in a
milk processing plant.
Milk with pH v alues higher than pH 6.7 po tentially indicat e that the milk may have c ome
from cow s with a mastit is infection. M astitis is an eve r-present chall enge with dair y milking
cows. Wh en infec ted, the c ow's immun e syste m releas es histam ine and othe r compounds
in respons e to the infect ion. There is a resul ting increase in p ermeability of e ndothelial and
epitheli al cell layers, allow ing blood component s to pass through a par acellular p athway.
Sinceblo od plasma is slightl y alkaline, the resul ting pH of milk will be hig her than normal.
Typically mi lk producers ca n perform a so matic cell cou nt to detect a mas titis infec tion, but a
pH measure ment offer s a quick way to sc reen for infe ction.
Underst anding the p H of raw milk can also help pr oducers o ptimize t heir proc essing
techniqu es. For example, in ope rations that use Ul tra High Temperature (U HT) processing ,
even small v ariations from pH 6.7 can a ffect the time r equired for paste urizati on and the
stabilit y of the milk af ter treatm ent.
Probe Features
Low Temperature Glass Tip and Open Junction.
The glass t ip uses a special l ow temperatur e (LT) glass
formulat ion. This is benef icial since many f ood product s are at
low temper atue. The open ju nction design c onsist of a solid g el
interf ace between t he sample and the in ternal refere nce wire.
This makes i t impermeable to c logging afte r measurement s.
Conical Glass Tip
The conical shaped tip design allows for penetration into solids, semi-solids, and emulsions
for the direct measurement of pH in samples such as milk and yogurt.
Liquid Cr ystal
Display
ON/OFF
Button
Maximum
Immersio n
Level
pH Probe
Calibration
tags
Battery
indicator
Unstable
tag
Measurement
unit
Digits
Recommended
Immersion
Level
Range 0.0 to 14.0 pH
Resolution 0 .1 pH
Accuracy ±0.2 pH @25 °C/ 77 °F
Calibration Automatic, one or two-point
Electrode Built-in probe for specific application
Batte ry Type CR2032 Li-ion
Batter y Life Approxi mately 1000 hour s of continuous us e
Auto-off 8 minutes, 6 0 minutes or can be di sabled
Environment 0 to 50 °C (32 to 122 °F); R H 95% max
Dimensions 51 x 159 x 21 mm (2 x 6.3 x 0.9“)
Weight 5 0 g (1.8 oz.)
Specifications
"Sto" will b e
displayed when
the calibr ation
is saved.