Hanna Instruments HI97700 Instruction Manual

INSTRUCTION MANUAL

HI97700

Ammonia Low

Range

Photometer

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the

instrument.
This manual will provide you with the necessary information for correct

use of the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to

e‑mail us at [e-mail] or view our worldwide contact
list at www.hannainst.com.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

Dear
Customer,

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TABLE OF CONTENTS

1. PRELIMINARY EXAMINATION ........................................................................

2. SAFETY MEASURES ......................................................................................

3. SPECIFICATIONS ..........................................................................................

4. ABBREVIATIONS ..........................................................................................

5. DESCRIPTION ..............................................................................................

5.1. GENERAL DESCRIPTION AND INTENDED USE ...........................................

5.2. FUNCTIONAL DESCRIPTION ....................................................................

5.3. PRECISION AND ACCURACY ....................................................................

5.4. PRINCIPLE OF OPERATION .....................................................................

5.5. OPTICAL SYSTEM ..................................................................................

6. GENERAL OPERATIONS .................................................................................

6.1. METER VALIDATION: CAL CHECK / CALIBRATION ......................................

6.2. CHEMICAL FORMULA / UNIT CONVERSION ..............................................

6.3. GLP .....................................................................................................

6.4. LOGGING DATA/LOG RECALL ...................................................................

6.5. GENERAL SETUP ...................................................................................

6.6. REAGENTS/ACCESSORIES ......................................................................

6.7. CONTEXTUAL HELP ................................................................................

6.8. BATTERY MANAGEMENT ........................................................................

7. PHOTOMETER ..............................................................................................

7.1. PROPER USE OF DROPPER BOTTLE .........................................................

7.2. CUVETTE PREPARATION .........................................................................

8. METHOD PROCEDURE ..................................................................................

9. ERROR DESCRIPTIONS .................................................................................

10. BATTERY REPLACEMENT .............................................................................

11. ACCESSORIES ............................................................................................

11.1. REAGENT SETS ...................................................................................

11.2. OTHER ACCESSORIES ..........................................................................

CERTIFICATION ................................................................................................

RECOMMENDATIONS FOR USERS ......................................................................

WARRANTY .....................................................................................................

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1. PRELIMINARY EXAMINATION

Remove the instrument and accessories from the packaging and examine it carefully to
make sure that no damage has occurred during shipping. Notify your nearest Hanna

Customer Service Center if damage is observed.
Each HI97700C is supplied with:

• Sample cuvette (2 pcs.)

• Sample cap (2 pcs.)

• Plastic stopper (2 pcs.)

• A ZERO ‑ CAL Check Cuvette A

• HI97700B ‑ CAL Check Cuvette B for Ammonia LR

• Cloth for wiping cuvettes

• Scissors

• Alkaline batteries: 1.5V AA (3 pcs.)

• Instruction manual

• Meter quality certificate

• CAL Check standard certificate

Each HI97700 is supplied with:

• Sample cuvette (2 pcs.)

• Sample cap (2 pcs.)

• Plastic stopper (2 pcs.)

• Alkaline batteries: 1.5V AA (3 pcs.)

• Instruction manual

• Meter quality certificate

Note: Save all packing material until you are sure that the instrument works correctly.
Any damaged or defective item must be returned in its original packing material with the
supplied accessories.

2. SAFETY MEASURES

• The chemicals contained in the reagent kits may be hazardous if
improperly handled.

• Read the Safety Data Sheets (SDS) before performing tests.

• Safety equipment: Wear suitable eye protection and clothing when required, and
follow instructions carefully.

• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty
of water. If reagent contacts skin, rinse the affected area thoroughly with water.
Avoid breathing released vapors.

• Waste disposal: For proper disposal of reagent kits and reacted samples, contact
a licensed waste disposal provider.

PRELIMINARY EXAMINATIONSAFETY MEASURES

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Ammonia
Low Range

Range 0.00 to 3.00 mg/L (as NH3‑N)
Resolution 0.01 mg/L

Accuracy ±0.04 mg/L ±4% of reading at 25 °C

Method

Adaptation of the ASTM Manual of Water
and Environmental Technology,
D1426 Nessler method

Measurement
System

Light source Light Emitting Diode
Bandpass filter 420 nm
Bandpass filter

bandwidth

8 nm

Bandpass filter
wavelength accuracy

±1.0 nm

Light detector Silicon photocell
Cuvette type Round 24.6 mm diameter (22 mm inside)

Additional
Specifications

Auto logging 50 readings
Display 128 x 64 pixel B/W LCD with backlight

Auto‑off

After 15 minutes of inactivity

(30 minutes before a READ measurement)
Battery type Alkaline 1.5 V AA (3 pcs.)
Battery life > 800 measurements (without backlight)

Environment

0 to 50 °C (32 to 122 °F);

0 to 100% RH, non‑serviceable
Dimensions 142.5 x 102.5 x 50.5 mm (5.6 x 4.0 x 2.0")
Weight (with batteries) 380 g (13.4 oz.)
Case ingress protection

rating

IP 67, floating case

3. SPECIFICATIONS

SPECIFICATIONS

4. ABBREVIATIONS

mg/L milligrams per liter (ppm)
mL milliliter
°C degree Celsius
°F degree Fahrenheit
LED Light Emitting Diode
HDPE High density polyethylene
GLP Good Laboratory Practice

ABBREVIATIONS

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DESCRIPTION

5. DESCRIPTION

5.1. GENERAL DESCRIPTION AND INTENDED USE

The HI97700 is an auto‑diagnostic portable meter that benefits from Hanna’s years
of experience as a manufacturer of analytical instruments. It has an advanced optical
system that uses a Light Emitting Diode (LED) and a narrow band interference filter
that allows for accurate and repeatable readings.

The optical system is sealed from outside dust, dirt and water. The meter uses an
exclusive positive‑locking system to ensure that the cuvettes are placed into the holder
in the same position every time.

With the CAL Check functionality, users are able to validate the performance of the
instrument at any time and apply a user calibration (if necessary). Hanna's CAL Check
cuvettes are made with NIST traceable standards.

The built in Tutorial mode guides users step‑by‑step through the measurement process. It
includes all steps required for sample preparation, the required reagents and quantities.

The HI97700 meter measures ammonia in water samples from 0.00 to 3.00 mg/L
(ppm). The method is an adaptation of the ASTM Manual of Water and Environmental
Technology, D1426 Nessler method.

Ammonia is an indication of animal and/or plant decay. It is one of the important
pollutants monitored in natural water due to its toxicity.

The HI97700 photometer is a compact and versatile meter suitable for field or bench
measurements, featuring a:

• Sophisticated optical system

• Meter Validation using Certified CAL Check cuvettes

• Tutorial Mode guides user step‑by‑step

• Auto logging

• Waterproof IP67, floating case

• GLP Features

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Keypad Description

The keypad contains 3 direct keys and 3 functional keys with the following functions:

Press the functional keys to perform the function displayed above them on the LCD.

Press and hold to power off/on. Press briefly to return to the previous screen.

Press to access the menu screen.

Press to display the context‑sensitive help menu.

DESCRIPTION

1) ON/OFF power button 3) Liquid Crystal Display (LCD) 5) Indexing mark

2) Keypad 4) Cuvette holder 6) Battery cover

5.2. FUNCTIONAL DESCRIPTION

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DESCRIPTION

5.4. PRINCIPLE OF OPERATION

Absorption of light is a typical phenomenon of interaction between electromagnetic
radiation and matter. When a light beam crosses a substance, some of the radiation
may be absorbed by atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical
path length through the matter and on the physical‑chemical characteristics of the
substance according to the Lambert‑Beer Law:

‑log I/Io = el c d

or

A = el c d

Io = intensity of incident light beam
I = intensity of light beam after absorption
e

l

= molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance of the substance
as the other factors are constant.
Photometric chemical analysis is based on specific chemical reactions between a
sample and reagent to produce a light‑absorbing compound.

5.3. PRECISION AND ACCURACY

Precision is how closely repeated
measurements are to one another.
Precision is usually expressed as
standard deviation (SD).
Accuracy is defined as the
closeness of a test result to the
true value.
Although good precision suggests
good accuracy, precise results can
be inaccurate. The figure explains
these definitions.
For each method, the accuracy is expressed in the related measurement section.

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DESCRIPTION

Instrument Block Diagram

The internal reference system (reference detector) of the HI97700 photometer
compensates for any drifts due to power fluctuations or ambient temperature changes,
providing a stable source of light for your blank (zero) measurement and sample
measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have
a much higher luminous efficiency, providing more light while using less power. They
also produce little heat, which could otherwise affect electronic stability. LEDs are
available in a wide array of wavelengths, whereas tungsten lamps have poor blue/
violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter,
stronger signal to be received. The end result is higher measurement stability and less
wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from
cuvette imperfections and scratches, eliminating the need to index the cuvette.

5.5. OPTICAL SYSTEM

GENERAL OPERATIONS

6.1. METER VALIDATION: CAL CHECK / CALIBRATION

Validation of the HI97700 involves verifying the concentration of the certified CAL
Check standards. The CAL Check screen guides the user step‑by‑step through the
validation process and user calibration (if necessary).

WARNING: Do not use any solutions/standards other than the HANNA® CAL Check
Standards. For accurate validation and calibration results, please perform these at
room temperature (18 to 25 °C; 64.5 to 77.0 °F).

Note: CAL Check Standards will not read the specified value in measurement mode.
Protect the CAL Check cuvettes from direct sunlight by keeping them in the original
packing. Store between +5 °C and +30 °C (41 - 86 °F), do not freeze.

6. GENERAL OPERATIONS

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GENERAL OPERATIONS

To perform a CAL Check:

1. Press CAL Check key from measurement mode.
The “Not Available“ message or the date/time

and status of the last CAL Check will be displayed
on the screen.

2. Press the Check key to start a new CAL Check.

Press the key at any time to abort the
validation process.

3. Use the   keys to enter the certificate value

of the calibration standard found on the CAL Check
Standard Certificate. Press Next to continue.

Note: This value will be saved in the instrument for future validation. If a new

set of calibration standards is obtained please update the certificate value.

4. Insert the A ZERO CAL Check Cuvette A then press

Next to continue. The “Please Wait...“ message
will be displayed during the measurement.

5. Insert the HI97700B CAL Check Cuvette B then press

Next to continue. The “Please Wait...“ message
will be displayed during the measurement.

6. When the CAL Check is complete the display will
show one of the following messages and the value
obtained during the measurement:

‑ “PASSED“: The measured value is within the accuracy

specification, no user calibration is required.

‑ “OUT OF SPECIFICATION“ and the Calibration key is available: The measured

value is near the expected value. To update the user calibration press Calibrate.
Press Accept to confirm or Cancel to return to the previous screen.

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GENERAL OPERATIONS

6.4. LOGGING DATA/LOG RECALL

The instrument features a data autolog function to help users track of all measurements.
Every time a measurement is made the data is automatically saved. The data log can
hold 50 individual measurements. When the 50 measurements are full and there are no
deleted data, the meter will rewrite the oldest log.
Viewing and deleting the data is possible using the Log Recall menu.

Press the key to enter the menu. Use the functional   keys to select Log
Recall and press Select.

6.3. GLP

Press the key to enter the menu. Use the  
keys to select GLP and press Select. Good Laboratory
Practice (GLP) shows the date and time of the last
user calibration (if available) or factory calibration. To
erase the last user calibration and to clear the CAL Check press Clear and follow the
prompts. Press Yes to erase and return to the factory calibration data or No to exit the
clear procedure.

‑ “OUT OF SPECIFICATION“: A user calibration is not

allowed, the measured value is outside of the toler‑
ance window. Check the certified value, expiration
date and clean the outside of the cuvette. Repeat the
CAL Check procedure. If this error continues contact your nearest Hanna Customer
Service Center.

6.2. CHEMICAL FORMULA / UNIT CONVERSION

Chemical formula/unit conversion factors are pre‑programmed into the instrument
and are method specific. In order to view the displayed result in the desired chemical

formula enter menu by pressing and use the   keys to select Chemical
Form. Press Select to change the displayed chemical form. Use the   keys to
highlight the desired chemical formula and press Select. The selected formula will be
saved when the instruments is powered off.

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Backlight

Values: 0 to 100 %

Press the Modify key to access the backlight intensity.
Use the   keys to increase or decrease the value.

Press the Accept key to confirm or the key to
return to the Setup menu without saving the new
value.

GENERAL OPERATIONS

Contrast

Values: 0 to 100 %

Press the Modify key to change the display’s contrast.
Use the   keys to increase or decrease the value.

Press the Accept key to confirm the value or the
key to return to the Setup menu without saving the
new value.

6.5. GENERAL SETUP

Press the key to enter the menu. Use the   keys to select Setup and press
Select. Use the   keys to highlight desired option.

Press No or the key to return to the previous screen.
Press Yes to delete selected log.
Press Del All to erase all the logged data.
If Del All is pressed follow the prompt to confirm.

Press Yes to delete all logged data, No or the key to return to the log recall.

Use the functional   keys to highlight a log and press Info to view additional
information about the log. From this screen the Next and Previous keys can be used
to view other logs.

Use the Delete key to erase logged data. After pressing Delete a prompts on display
is asking for confirmation.

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Date / Time

Press the Modify key to change the date/time.
Press the   keys to highlight the value to be
modified (year, month, day, hour, minute or second).
Press Edit to modify the highlighted value. Use the
  keys to change the value.

Language

Press the Modify key to change the Language. Use
the   keys to select the desired language.
Press Accept to choose one of the languages installed.

Date Format

Press the Modify key to change the date format.
Use the   keys to select the desired format.

Press the Accept key to confirm or the key to
return to the Setup menu without saving the new
format.

Time Format

Option: AM/PM or 24-hour Press the functional key
to select the desired time format.

GENERAL OPERATIONS

Decimal Separator

Option: Comma ( , ) or Period ( . )

Press the functional key to select the desired decimal
separator. The decimal separator is used on the
measurement screen.

Press the Accept key to confirm or the key to
return to the previous screen.

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Meter Information

Press the Select key to view the model, serial number,
firmware version and selected language. Press the

key to return to the Setup menu.

Restore factory settings

Press the Select key to reset to factory settings.
Press Accept to confirm or Cancel to exit without
restoring the factory settings.

GENERAL OPERATIONS

6.6. REAGENTS/ACCESSORIES

Press the key to enter the menu. Use the 
 keys to select Reagents/Accessories and press
Select to access a list of reagents and accessories. To

exit press the key.

Beeper

Option: Enable or Disable

When enabled, a short beep is heard every time a
key is pressed. A long beep alert sounds when the
pressed key is not active or an error is detected. Press
the functional key to enable/disable the beeper.

Tutorial

Option: Enable or Disable

When enabled, the user will be guided step‑by‑step
through the measurement procedure.

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GENERAL OPERATIONS

6.8. BATTERY MANAGEMENT

The meter will perform an auto‑diagnostic test when it is powered on. During this test,
the HANNA® logo will appear on the LCD. After 5 seconds, if the test was successful,
the last method selected will appear on the display. The battery icon on the LCD will
indicate the battery status:

‑ battery full

‑ battery below 10%, replace the batteries soon

‑ battery is low, replace the batteries with new ones

To conserve battery, the meter will turn off automatically after 15 minutes of inactivity.
If a zero reading has been done but not a read, auto‑off time is increased to 30 minutes.

6.7. CONTEXTUAL HELP

The HI97700 offers an interactive contextual help
mode that assists the user at any time.

To access the help screen press the key.
The instrument will display additional information
related to the current screen. To read all the available information, scroll the text using
the   keys.

To exit help mode press the or the key and the meter will return to the
previous screen.

7.1. PROPER USE OF DROPPER BOTTLE

(a) For reproducible results, tap the dropper on the table several

times and wipe the outside of the tip
with a cloth.

(b) Always keep the dropper bottle in

a vertical position while dosing the
reagent.

7. PHOTOMETER

PHOTOMETER

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PHOTOMETER

7.2. CUVETTE PREPARATION

Proper mixing is very important for reproducibility of the measurements.
Invert the cuvette a couple of times or for a specified time: hold the cuvette in the
vertical position. Turn the cuvette upside‑down and wait for all of the solution to flow
to the cap end, then return the cuvette to the upright vertical position and wait for all of
the solution to flow to the cuvette bottom. This is one inversion. The correct speed for this
mixing technique is 10‑15 complete inversions in 30 seconds.
This mixing technique is indicated with “invert to mix” and the following icon:

In order to avoid reagent leaking and to obtain more accurate
measurements, close the cuvette first with the supplied HDPE plastic
stopper and then the black cap.
Whenever the cuvette is placed into the measurement holder, it must be
dry outside and free of fingerprints, oil or dirt. Wipe it thoroughly with

HI731318 or a lint‑free cloth prior to insertion.

Shaking the cuvette can generate bubbles in the sample, causing higher
readings. To obtain accurate measurements, remove such bubbles by swirling or by
gently tapping the cuvette.
Do not let the reacted sample stand too long after reagent
is added. For best accuracy, respect the timings described in
each specific method.
It is possible to take multiple readings in a row, but it is
recommended to take a new zero reading for each sample
and to use the same cuvette for zeroing and measurement
when possible.
Discard the sample immediately after the reading is taken, or the glass might become
permanently stained.
All the reaction times reported in this manual are at 25 °C (77 °F). In general, the
reaction time should be increased for temperatures lower than 20 °C (68 °F), and
decreased for temperatures higher than 25 °C (77 °F).

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METHOD PROCEDURE

10 mL

8. METHOD PROCEDURE

REQUIRED REAGENTS
Code Description Quantity

HI93700A‑0 Ammonia Low Range Reagent A 4 drops
HI93700B‑0 Ammonia Low Range Reagent B 4 drops

REAGENT SETS

HI93700‑01 Ammonia LR Reagent ‑ 100 tests
HI93700‑03 Ammonia LR Reagent ‑ 300 tests

For other accessories see page 22.

MEASUREMENT PROCEDURE

Note: If tutorial mode is disabled follow the measurement procedure below. If the
tutorial mode is enabled, press Measure and follow the messages on the screen.

• Fill the cuvette with 10 mL of unreacted sample (up to
the mark). Replace the plastic stopper and the cap.

• Place the cuvette into the holder and ensure that the
notch on the cap is positioned securely into the groove.

• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and
ready for measurement.

• Remove the cuvette.

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METHOD PROCEDURE

• Add 4 drops of HI93700A‑0 Ammonia Low Range
Reagent A. Replace the plastic stopper and the
cap. Invert 5 times to mix.

• Add 4 drops of HI93700B‑0 Ammonia Low Range
Reagent B. Replace the plastic stopper and the cap.
Invert 5 times to mix.

• Replace the cuvette into the holder and ensure that the
notch on the cap is positioned securely into the groove.

• Press the Read key and the display will show the countdown 3 minutes and 30
seconds prior to the measurement. To skip the timer press Read twice. When the
timer ends the meter will perform the reading. The instrument displays the results
in mg/L of ammonia nitrogen (NH3-N).

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METHOD PROCEDURE

• Press and use the   keys to select Chemical Form.

• Press Select to change the displayed chemical form to mg/L of ammonia (NH3)

and ammonium (NH

4

+

).

INTERFERENCE

Interference may be caused by:
Acetone
Alcohols
Aldehydes
Glycine
Hardness above 1 g/L
Iron
Organic chloramines
Sulfide
Various aliphatic and aromatic amines

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ERROR DESCRIPTIONS

The instrument shows clear warning messages when erroneous conditions appear and when
measured values are outside the expected range. These messages are described below.

No Light: The light source is not functioning properly.

Light Leak: There is an excess amount of ambient light

reaching the detector.

Inverted Cuvette: The sample and the zero cuvettes
are inverted.

Light Low: The instrument cannot adjust the light
level. Please check that the sample does not contain
any debris.

Light High: There is too much light to perform a
measurement. Please check the preparation of the
zero cuvette.

9. ERROR DESCRIPTIONS

Ambient temperature out of limits: The meter is too
hot or too cold for an accurate measurement. Allow
the meter to reach 10 °C to 40 °C (50 °F to 104 °F)
before performing a measurement.

Ambient temperature changed: The temperature
of the meter has changed significantly since the
zero measurement has been performed. A zero
measurement must be performed again.

Out of range: The measured value is outside the limits
of the method.

Battery Low: Battery is low, replace the batteries with
new ones.

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To replace the instrument’s batteries, follow these steps:

• Turn the instrument off by pressing and holding the key.

• Remove the battery cover by turning it counterclockwise.

• Remove the old batteries, replace them with three new 1.5V AA batteries.

• Replace the battery cover, turn it clockwise to close.

10. BATTERY REPLACEMENT

BATTERY REPLACEMENT

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MAN97700 08/18

11. ACCESSORIES

11.1. REAGENT SETS

Code Description
HI93700‑01 Ammonia LR Reagent ‑ 100 tests

HI93700‑03 Ammonia LR Reagent ‑ 300 tests

11.2. OTHER ACCESSORIES

Code Description
HI731318 cloth for wiping cuvettes (4 pcs.)

HI731331 glass cuvettes (4 pcs.)
HI731336N cap for cuvette (4 pcs.)
HI97700‑11 CAL Check® standards for ammonia LR ‑ cuvette kit
HI93703‑50 cuvette cleaning solution (230 mL)

ACCESSORIES

Hanna Instruments reserves the right to modify the design, construction or appearance of

its products without advance notice.

All Hanna Instruments conform to the CE European Directives.

Disposal of Electrical & Electronic Equipment. The product should
not be treated as household waste. Instead hand it over to the
appropriate collection point for the recycling of electrical and electronic
equipment which will conserve natural resources.

Disposal of waste batteries. This product contains batteries, do not
dispose of them with other household waste. Hand them over to the
appropriate collection point for recycling.

Ensuring proper product and battery disposal prevents potential
negative consequences for the environment and human health, which
may be caused by inappropriate handling. For more information,
contact your city, your local household waste disposal service, the
place of purchase or go to www.hannainst.com.

RoHS

compliant

The HI97700 is warranted for two years against defects in workmanship and
materials when used for its intended purpose and maintained according
to instructions. This warranty is limited to repair or replacement free of
charge. Damage due to accidents, misuse, tampering or lack of prescribed
maintenance is not covered.

If service is required, contact your local Hanna Instruments Office. If under
warranty, report the model number, date of purchase, serial number
(engraved on the bottom of the meter) and the nature of the problem. If
the repair is not covered by the warranty, you will be notified of the charges
incurred. If the meter is to be returned to Hanna Instruments, first obtain
a Returned Goods Authorization (RGA) number from the Technical Service
department and then send it with shipping costs prepaid. When shipping any
meter, make sure it is properly packed for complete protection.

Warranty

Before using this product, make sure it is entirely suitable
for your specific application and for the environment in
which it is used. Any variation introduced by the user to the
supplied equipment may degrade the meters’ performance.
For yours and the meter’s safety do not use or store the
meter in hazardous environments.

Recommendations for
Users

Certification

Printed in ROMANIA

MAN97700

World
Headquarters

Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
www.hannainst.com

Local Office

Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
Phone: [phone]
Fax: [phone]
e‑mail: [e-mail]