Hanna Instruments HI96801 User Manual

Instruction Manual

HI 96801

Refractometer for Sucrose

Measurements

www.hannainst.com

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Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary

information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at [e-mail].

This instrument is in compliance with

directives.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ............................................................................................. 2

GENERAL DESCRIPTION ..................................................................................................... 3

SPECIFICATIONS .................................................................................................................. 3

PRINCIPLE OF OPERATION ................................................................................................4

FUNCTIONAL DESCRIPTION................................................................................................ 5

DISPLAY ELEMENTS ............................................................................................................ 6

WARNINGS ...........................................................................................................................6

ERROR MESSAGES .............................................................................................................. 7

CALIBRATION PROCEDURE ................................................................................................8

MEASUREMENT PROCEDURE .............................................................................................9

MAKING A STANDARD BRIX SOLUTION ..........................................................................10

CHANGING TEMPERATURE UNITS ................................................................................... 10

BATTERY REPLACEMENT .................................................................................................11

WARRANTY ........................................................................................................................11

GENERAL DESCRIPTION

The HI 96801 Digital Sucrose Refractometer is a rugged portable, water resistant device that
benefits from Hanna’s years of experience as a manufacturer of analytical instruments.

The HI 96801 is an optical instrument that employs the measurement of refractive index to
determine the % Brix of sugar in aqueous solutions. The method is both simple and quick. Samples
are measured after a simple user calibration with deionized or distilled water. Within seconds the
instrument measures the refractive index of the sample and converts it to % Brix concentration units.
The HI 96801 digital refractometer eliminates the uncertainity associated with mechanical
refractometers and is easily portable for measurements in the field.

The measurement technique and temperature compensation employ methodology recommended in
the ICUMSA Methods Book (Internationally recognized body for Sugar Analysis).

Temperature (in °C or °F) is displayed simultaneously with the measurement on the large dual level
display along with icons for Low Power and other helpful message codes.

Key features include:

• Dual-level LCD

• Automatic Temperature Compensation (ATC)

• Easy setup and storage

• Battery operation with Low Power indicator (BEPS)

• Automatically turns off after 3 minutes of non-use.

PRELIMINARY EXAMINATION

Remove the instrument from the packing materials and examine carefully to ensure no
damage has occurred during shipping. If any damage has occurred, notify your Dealer
or closest Hanna Customer Service Center.

Each HI 96801 instrument is supplied with:

• 9 V battery

• Instruction manual

Note:Save all packing material until you are sure that the instrument functions

correctly. A defective instrument must be returned in its original packing.

MODEL IDENTIFICATION
HI 96801

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the
copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

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SPECIFICATIONS

Range: 0 to 85% Brix / 0 to 80°C (32 to 176°F)
Resolution: ± 0.1% Brix / ±0.1°C (±0.1°F)
Accuracy: ± 0.2% Brix / ± 0.3°C (±0.5°F)
Temperature Compensation: Automatic between 10 and 40°C (50 - 104°F)
Measurement Time: Approximately 1.5 seconds
Minimum Sample Volume: 100μL (cover prism totally)
Light Source: Yellow LED
Sample Cell: SS ring and flint glass prism
Case Material: ABS
Enclosure Rating: IP 65
Battery Type / Life: 1 X 9V / 5000 readings
Auto-Off: After 3 minutes of non-use
Dimensions: 19.2(W) x 10.2(D) x 6.7 (H)cm
Mass: 420g

The Brix determination is made by measuring the refractive index of a solution. Refractive Index is
an optical characteristic of a substance and the number of dissolved particles in it. Refractive Index
is defined as the ratio of the speed of light in empty space to the speed of light in the substance. A
result of this property is that light will “bend”, or change direction, when it travels through a
substance of different refractive index. This is called refraction.
When passing from a material with a higher to lower refractive index, there is a critical angle at
which an incoming beam of light can no longer refract, but will instead be reflected off the interface.
The critical angle can be used to easily calculate the refractive index according to the equation:

FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION

FUNCTIONAL DESCRIPTIONPRINCIPLE OF OPERATION

FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION

TOP VIEW

sin (θ

Where n2 is the refractive index of the lower-density medium; n1 is the refractive index of the
higher-density medium.

In the HI 96801, light from an LED passes through a prism in contact with the sample. An image
sensor determines the critical angle at which the light is no longer refracted through the sample.
The HI 96801 automatically applies temperature compensation to the measurement and converts
the refractive index of the sample to sucrose concentration in units of percent (by weight) Brix.

) = n2 / n

critical

1

1. Liquid Crystal Display (LCD)

2. Read Key (User Measurement)

3. Zero Key (User Calibration)

4. On/Off

5. Primary Display

6. Secondary Display

7. Stainless Steel Sample Well and Prism

BOTTOM

8. Battery Cover

9. Battery Compartment

4 5

DISPLAY ELEMENTSDISPLAY ELEMENTS

DISPLAY ELEMENTS

DISPLAY ELEMENTSDISPLAY ELEMENTS

ERROR MESSAGESERROR MESSAGES

ERROR MESSAGES

ERROR MESSAGESERROR MESSAGES

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1. SETUP: Factory Calibration Tag

2. Measurement in Progress Tag

3. Battery (blinks when low battery condition detected)

4. Calibration Tag

5. Automatic Temperature Compensation
(blinks when temperature exceeds 10-40°C / 50-104°F range)

6 Primary Display (displays measurement and error messages)

7. Temperature Units

8. Secondary Display (displays temperature measurements; when blinking,
temperature has exceeded operation range: 0-80°C / 32-176°F)

MEASUREMENT GUIDELINESMEASUREMENT GUIDELINES

MEASUREMENT GUIDELINES

MEASUREMENT GUIDELINESMEASUREMENT GUIDELINES

• Handle instrument carefully. Do not drop.

• Do not immerse instrument under water.

• Do not spray water to any part of instrument except the “sample well” located over the prism.

• The instrument is intended to measure Sucrose solutions. Do not expose instrument or prism

to solvents that will damage it. This includes most organic solvents and extremely hot or cold
solutions.

• Particulate matter in a sample may scratch the prism. Absorb sample on soft tissue and rinse

sample well with deionized or distilled water between samples.

• Use plastic pipettes to transfer all solutions. Do not use metallic tools such as needles, spoons

or tweezers as these will scratch the prism.

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CALIBRATIONCALIBRATION

CALIBRATION

CALIBRATIONCALIBRATION

MEASUREMENTMEASUREMENT

MEASUREMENT

MEASUREMENTMEASUREMENT

Calibration should be performed daily, before measurements are made, when the battery has
been replaced, or between a long series of measurements.

1. Press the ON/OFF key, then release. Two instrument test screens will be displayed briefly;
a LCD segment followed by the percentage of remaining battery life. When LCD displays dashes,
the instrument is ready.

2. Using plastic pipettes, fill the sample well with distilled or deionized water.
Note: If the ZERO sample is subject to intense light such as sunlight or another strong source,

cover the sample well with your hand or other shade during the calibration.

3. Press the ZERO key. If no error messages appear, your unit is calibrated.
(For a description of ERROR MESSAGES see page 7).
Note: The 0.0 screen will remain until a sample is measured or the power is turned off.

Verify the instrument has been calibrated before taking measurements.

1. Wipe off prism surface located at the bottom of the sample well.

2. Using plastic pipettes, drip sample onto the prism surface. Fill the well completely.
Note: If the temperature of the sample differs significantly from the temperature of the

instrument, wait approximately 1 minute to allow thermal equilibration.

3. Press the READ key. Measurement is displayed in units of % BRIX.

4. Gently absorb the ZERO water standard with a soft tissue. Use care not to scratch the prism
surface. Wipe off the surface completely. The instrument is ready for sample measurement.
Note: If instrument is turned off the calibration will not be lost.

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Note: The ATC tag blinks and automatic temperature compensation is disabled if the

temperature exceeds the 10-40°C / 50-104°F range.

4. Remove sample from the sample well by absorbing on a soft tissue.

5. Using plastic pipettes, rinse prism and sample well with distilled or deionized water. Wipe
dry. The instrument is ready for the next sample.

MAKING A STANDARD % BRIX SOLUTIONMAKING A STANDARD % BRIX SOLUTION

MAKING A STANDARD % BRIX SOLUTION

MAKING A STANDARD % BRIX SOLUTIONMAKING A STANDARD % BRIX SOLUTION

To make a Brix Solution, follow the procedure below:

• Place container (such as a

• Tare the balance.

• To make an X BRIX solution weigh out X grams of high purity Sucrose (CAS #: 57-50-1)

directly into the container.

• Add distilled or deionized water to the container so the total weight of the solution is 100g.
Note: Solutions above 60% Brix need to be vigorously stirred or shaken and heated in a water

•

bath. Remove solution from bath when sucrose has dissolved. The total quantity can be
scaled proportionally for smaller containers but accuracy may be sacrificed.

Example with 25% Brix:
%Brix%Brix

%Brix

%Brix%Brix
25 25.000 75.000 100.000

TO CHANGE TEMPERATURE UNITSTO CHANGE TEMPERATURE UNITS

TO CHANGE TEMPERATURE UNITS

TO CHANGE TEMPERATURE UNITSTO CHANGE TEMPERATURE UNITS

To change the temperature measurement unit from Celsius to Fahrenheit (or vice versa), follow this
procedure.

1. Press and hold the ON/OFF key continuously for aproximately 15 seconds. The LCD will

display the “all segment” screen followed by a screen with the model number on the primary
display and the version number on the secondary display. Continue pressing the ON/OFF key.

glass vial or dropper bottle that has a cover) on an analytical balance.

g Sucroseg Sucrose

g Sucrose

g Sucroseg Sucrose

g Waterg Water

g Water

g Waterg Water

g Tg T

g T

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BATTERY REPLACEMENTBATTERY REPLACEMENT

BATTERY REPLACEMENT

BATTERY REPLACEMENTBATTERY REPLACEMENT

To replace the instrument’s battery, follow these steps:

• Turn the instrument OFF by pressing the ON/OFF key.

• Turn instrument upside down and remove the battery cover by turning it counterclockwise.

• Extract the battery from its location.

• Replace with fresh 9V battery making certain to observe polarity.

• Insert the back battery cover and fasten it by turning clockwise to engage.

15 seconds

2. While continuing to hold the ON/OFF key, press the ZERO key. The temperature unit will

change from °C to °F or vice versa.

°C or °F

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WARRANTYWARRANTY

WARRANTY

WARRANTYWARRANTY

HI 96801 is warranted for two years against defects in workmanship and materials when used
for its intended purpose and maintained according to the instructions.

This warranty is limited to repair or replacement free of charge.
Damage due to accident, misuse, tampering or lack of prescribed maintenance is not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of

purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.

If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods
Authorization Number from the Customer Service Department and then send it with shipment
costs prepaid. When shipping any instrument, make sure it is properly packaged for complete
protection.

To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.

SALES AND TECHNICAL SERVICE CONTACTSSALES AND TECHNICAL SERVICE CONTACTS

SALES AND TECHNICAL SERVICE CONTACTS

SALES AND TECHNICAL SERVICE CONTACTSSALES AND TECHNICAL SERVICE CONTACTS

Australia:

Tel. [phone] • Fax [phone]

China:

Tel. [phone] • Fax [phone]

Egypt:

Tel. & Fax [phone]

Germany:

Tel. [phone] • Fax [phone]

Greece:

Tel. [phone] • Fax [phone]

Indonesia:

Tel. [phone] • Fax [phone]

Japan:

Tel. [phone] • Fax [phone]

Korea:

Tel. [phone] • Fax [phone]

Malaysia:

Tel. [phone] • Fax [phone]

Singapore:

Tel. [phone] • Fax [phone]

South Africa:

Tel. [phone] • Fax [phone]

Taiwan:

Tel. [phone] • Fax [phone]

Thailand:

Tel. [phone] • Fax [phone]

12/08

United Kingdom:

Tel. [phone] • Fax [phone]

USA:

Tel. [phone] • Fax [phone]

For e-mail contacts and a complete list of Sales and Technical offices, please see

www.hannainst.com

.

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MAN96801