Hanna Instruments HI 96785 User Manual

Instruction Manual

HI 96785C

Honey Color

Analyzer

www.hannainst.com

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Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary

information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at [e-mail].

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ................................................................................................ 3

GENERAL DESCRIPTION ....................................................................................................... 4

ABBREVIATIONS .................................................................................................................. 4

SIGNIFICANCE AND USE ...................................................................................................... 5

SPECIFICATIONS ................................................................................................................... 6

PRINCIPLE OF OPERATION ................................................................................................... 6

FUNCTIONAL DESCRIPTION .................................................................................................. 8

ERRORS AND WARNINGS .................................................................................................... 9

GENERAL TIPS FOR AN ACCURATE MEASUREMENT ............................................................. 11

STARTUP ........................................................................................................................... 12

MEASUREMENT PROCEDURE ............................................................................................. 12

BATTERY MANAGEMENT .................................................................................................... 14

BATTERY REPLACEMENT .................................................................................................... 14

ACCESSORIES ..................................................................................................................... 15

WARRANTY........................................................................................................................ 15

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.

Each HI 96785 Ion Selective Meter is supplied complete with:

• Five Sample Cuvettes

• One Light Shield Cap

• 30 mL Glycerol (1 bottle)

• 9V Battery

• Cloth for wiping cuvettes

• Instrument quality certificate

• Instruction Manual

• Rigid carrying case

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

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Note: Save all packing material until you are sure that the instrument works correctly. Any

defective item must be returned in its original packing.

GENERAL DESCRIPTION

SIGNIFICANCE AND USE

The HI 96785 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has the advanced optical
system based on two special tungsten lamps and two narrow band interference filters that allows
most accurate and repeatable readings. All instruments are factory calibrated and the electronic
and optical design minimizes the need of frequent calibration.

Display messages aid the user in routine operation. The meter has an auto-shut off feature that
will turn off the instrument after 10 minutes of non use in

The meter uses an exclusive positive-locking system to ensure that the cuvette is in the same
position every time it is placed into the measurement cell.

The HI 96785 measures the percent of light transmittance of honey compared to analytical
reagent grade glycerol. The instrument directly displays the measurement result expressed in
mm Pfund.

measurement mode

.

ABBREVIATIONS

Honey color varies naturally in a wide range of tonalities, ranging from light yellow to amber, dark
amber and black in extreme cases; sometimes green or red hues may also occur.
Color of untreated honey depends on botanical origin: for this reason color is very important for
definition and commercial classification of monofloral honeys. Honey darkens with aging, and other
changes in color may result from beekeeper’s interventions and from the different ways of conservation
(e.g.: use of old honeycombs, contact with metals, high temperatures, exposition to light, etc.).
The primary characteristic for commercial honey classification is color. Color classes are expressed in
millimeters (mm) Pfund grades, compared to an analytical grade Glycerol Standard Reference.
Table 1 reports the USDA classification for honey samples and the related mm Pfund values.
Table 2 shows the color of different monofloral honeys: data are obtained from a statistical set of
honey samples. The table reports for each type of honey: average value of color, standard deviation,
and the minimum and maximum values measured.

USDA Color Standards Designation Color Range Pfund Scale (mm)

Water White 8 or less
Extra White 8 to 17
White 17 to 34
Extra Light Amber 34 to 50
Light Amber 50 to 85
Amber 85 to 114
Dark Amber more than 114

mV:

mL:

LCD:

mm Pfund:

USDA:

millivolts
milliliter
Liquid Crystal Display
milliliters Pfund
US Department of Agriculture

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SPECIFICATIONS

Range 0 to 150 mm Pfund
Resolution 1 mm Pfund
Accuracy ±2 mm Pfund @ 80 mm Pfund
Typical EMC Deviation 1 mm Pfund
Light Source Tungsten lamp
Light Detector Silicon Photocell with narrow band interference filter @ 420 nm and

525 nm

Method Direct Measurement
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 1 x 9 volt
Auto-Shut off After 10' of non-use in

with last reading reminder.

Dimensions 192 x 102 x 67 mm (7.6 x 4 x 2.6")
Weight 290 g (10 oz.).

REQUIRED STANDARD
Description Quantity/test
Glycerol 4 mL

measurement mode

;

PRINCIPLE OF OPERATION

Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by
atoms, molecules or crystal lattices.

If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law:

-log I/Io = ελ c d

or

Where:

-log I/I

= Absorbance (A)

o

Io= intensity of incident light beam
I = intensity of light beam after absorption

ελ= molar extinction coefficient at wavelength λ

c = molar concentration of the substance
d = optical path through the substance

A = ελ c d

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are known.

Photometric chemical analysis is based on the possibility to develop an absorbing compound
from a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements.

The optical system of Hanna's HI 96 series colorimeters is based on special subminiature
tungsten lamps and narrow-band interference filters to guarantee both high performance and
reliable results.

HI 96 series block diagram (optical layout)

A microprocessor controlled special tungsten lamp emits radiation which is first optically
conditioned and beamed to the sample contained in the cuvette. The optical path is fixed by the
diameter of the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to
obtain a light beam of intensity Io or I.

The photoelectric cell collects the radiation I that is not absorbed by the sample and converts
it into an electric current, producing a potential in the mV range.

The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.

The measurement process is carried out in two phases: first the meter is zeroed and then the
actual measurement is performed.

The cuvette has a very important role because it is an optical element and thus requires
particular attention. It is important that both, the measurement and the calibration (zeroing)
cuvettes, are optically identical to provide the same measurement conditions. Whenever possible
use the same cuvette for both. It is necessary that the surface of the cuvette is clean and not
scratched. This to avoid measurement interference due to unwanted reflection and absorption of
light. It is recommended not to touch the cuvette walls with hands.

Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvette to prevent any contamination.

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FUNCTIONAL DESCRIPTION

ERRORS AND WARNINGS

INSTRUMENT DESCRIPTION

KEYPAD DESCRIPTION

• ON/OFF: to turn the meter on and off.

• CAL: to calibrate the meter prior to measurement.

• READ: to make a measurement.

OPERATING MODES

•

Measurement mode:

DISPLAY ELEMENTS DESCRIPTION

default operation mode, enables both calibration and measurement.

1) CAL key

2) READ key

3) ON/OFF key

4) Liquid Crystal Display (LCD)

5) Cuvette holder

The instrument shows clear messages when erroneous condition appears. Messages are also displayed
when the obtained values are outside expected range. The beeper is playing a beep on errors.

a) on calibration

Light High: There is too much light to perform a measurement.
Please check the preparation of the calibration cuvette.

Light Low: There is not enough light to perform a measurement.
Please check the preparation of the calibration cuvette.

No Light: The instrument cannot adjust the light level. Please
check that the calibration cuvette does not contain any debris.

1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or
reading measurement

2) Error messages and warnings

3) The battery icon shows the charging level of the battery

4) The hourglass appears when an internal checking is in progress

5) Status message

6) Four digit main display

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b) on sample reading

Inverted Cuvettes: The sample and the calibration cuvette are
inverted.

CAL: A Calibration reading was not taken. Follow the instructions
of the measurement procedure for calibrating the meter.

Under range: A blinking “0” indicates that the sample absorbs
less light than the calibration reference. Check the procedure and
recalibrate the instrument.

GENERAL TIPS FOR AN ACCURATE MEASUREMENT

The instructions listed below should be carefully followed during testing to ensure best accuracy.

d) other errors and warnings

Over Range: A flashing value of the maximum concentration
indicates an over range condition. The concentration of the
sample is beyond the programmed range.

Cap error: Appears when external light enters in the analysis
cell. Assure that the light shield cap is present.

Cooling lamp: The instrument waits for the lamp to cool down.

Battery low: The battery must be replaced soon.

• Use matched square cuvettes, having 4 clear faces and 10
mm path length.

• Do not touch the cuvette walls with hands.

• Whenever the cuvette is placed into the measurement cell, it
must be dry outside, and completely free of fingerprints, oil
or dirt. Wipe it thoroughly with HI 731318 or a lint-free
cloth prior to insertion.

• It is important that the sample does not contain any debris.
This would corrupt the reading.

• Samples should be completely free of air bubbles. If bubbles are present tap the cuvette on
the table to remove them.

• It is recommended to take the sample of honey in the middle of the container.

• It is recommended to take and analyze samples at room temperature.

• Do not use the same cuvette with glycerol for more than 5-10 times to perform calibration
and dispose of it after 1 day has passed.

Dead battery: This indicates that the battery is dead and must
be replaced. Once this indication is displayed, the meter will
lock up. Change the battery and restart the meter.

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• Never re-use honey sample cuvettes, dispose them after use.

Note: Honey samples exposed to direct sun light and/or air will darken over time. This is

caused by microorganisms.

STARTUP

Prepare the instrument for measurement as follows:

• Unpack the instrument by removing the dust protection sleeve from the instrument cuvette
holder.

• Place the battery in the instrument as described in the “BATTERY REPLACEMENT” chapter.

• Place the instrument on a flat table.

• Do not place the instrument under direct sun light.

MEASUREMENT PROCEDURE

• Press CAL and the lamp, cuvette and

detector icons will appear on the display,
depending on the measurement phase.

• After a few seconds, the display will

show “-0.0-”. The meter is now calibrated
and ready for measurement.

• Remove the Glycerol cuvette.

To compensate the meter for the sample turbidity or color, the measurement takes place in two
phases. First, the meter is calibrated using the glycerol. After the honey is measured.

• Turn the meter on by pressing ON/OFF.
The display briefly shows all tags on.

• When the beeper sounds briefly and the
LCD displays dashes, the meter is ready.
The blinking “CAL” indicates that the
instrument needs to be calibrated first.

• Fill one cuvette with 4 mL of Glycerol,
up to 5 mm (0.2’’) below the rim. This
is the Glycerol Standard Reference.

• Place the cuvette into the cuvette holder
paying attention to the direction of the
light indicated by the arrow on the
instrument. Then put the light shield cap
on and ensure that the notch on the cap
is positioned securely into the groove.

• Add to a second clean cuvette about 4 mL
of honey, up to 5 mm (0.2’’) below the
rim. This is the sample.

• Place the sample cuvette into the cuvette
holder paying attention to the direction
of the light indicated by the arrow on the
instrument. Then put the light shield cap
on and ensure that the notch on the cap
is positioned securely into the groove.

• Press READ
detector icons will appear on the display,
depending on the measurement phase.

• At the end of measurement, the instrument
directly displays the honey color intensity
value expressed in mm Pfund, as compared
to analytical grade Glycerol (fixed at
zero Pfund).

INTERFERENCES

Interference may be caused by air bubbles or turbidity in the sample. Scratched or dirty cuvettes
will also affect readings. Always check clearness of cuvettes prior to use.

and the lamp, cuvette and

WARRANTY

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BATTERY MANAGEMENT

ACCESORIES

To save battery, the instrument shuts down after 10 minutes of non-use in
If a valid measurement was displayed before auto

shut off, the value is displayed when the instrument
is switched on. The blinking “CAL” means that a
new calibration has to be performed.

One fresh battery lasts for around 350 measurements, depending on the light level.
The remaining battery capacity is evaluated at the instrument startup and after each measurement.
The instrument displays a battery indicator with three levels as follows:

• 3 lines for 100 % capacity

• 2 lines for 66 % capacity

• 1 line for 33 % capacity

• Battery icon blinking if the capacity is under 10 %.
If the battery is empty and accurate measurements can’t be taken anymore, the instrument

shows “dEAd bAtt” and turns off.
To restart the instrument, the battery must be replaced with a fresh one.

measurement mode.

BATTERY REPLACEMENT

To replace the instrument’s battery, follow the steps:

• Turn the instrument off by pressing ON/OFF.

• Turn the instrument upside down and remove the battery cover by turning it counterclockwise.

ANALYSIS KIT
C219/C220 Kit Kit for Honey Color analysis, including: 82 cuvettes, 30 mL of

Glycerol and two 5 mL syringe (75 tests average)

OTHER ACCESORIES
C115-00300 5 mL graduated syringe
HI 721310 9V battery (10 pcs.)
HI 731318 Cloth for wiping cuvettes (4 pcs.)
HI 731335 Caps for cuvettes
HI 741218 Carrying case

WARRANTY

HI 96785 is warranted for two years against defects in workmanship and materials when used
for its intended purpose and maintained according to the instructions.

This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of

purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.

If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization
Number from the Customer Service Department and then send it with shipment costs prepaid.
When shipping any instrument, make sure it is properly packaged for complete protection.

To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.

• Extract the battery from its location and replace it with a fresh one.

• Insert back the battery cover and turn it clockwise to close.

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Recommendations for Users

Before using these products, make sure that they are entirely suitable for your specific application and for the environment in
which they are used.

Operation of these instruments may cause unacceptable interferences to other electronic equipments, this requiring the operator
to take all necessary steps to correct interferences.

Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC performance.
To avoid damages or burns, do not put the instrument in microwave oven. For yours and the instrument safety do not use or
store the instrument in hazardous environments.

Hanna Instruments reserves the right to modify the design, construction and appearance of

its products without advance notice.

Hanna Instruments Inc.

Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA

Technical Support for Customers

Tel. [phone]
Fax [phone]
E-mail [e-mail]
www.hannainst.com

Local Sales and Customer Service Office

Printed in EUROPE

(ROMANIA)

MAN96785 01/11

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