Hanna Instruments HI 96769 User Manual

INSTRUCTION MANUAL

HI 96769HI 96769

HI 96769

HI 96769HI 96769

Anionic Surfactants

ISM

www.hannainst.com

Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.

Preliminary examination

Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occured during shipment, please notify your Dealer. Each HI 96769 Ion Selective Meter is supplied complete with:

• Two Sample Cuvettes and Caps

• 9V Battery

• Instruction Manual

Note: Save all packing material until you are sure that the instrument

works correctly. Any defective item must be returned in its original packing.

For more details about spare parts and accessories see “Accessories”

Technical specifications

Range 0.00 to 3.50 mg/L (as SDBS) Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±3% of reading @ 25°C Typical EMC Deviation ±0.01 mg/L Light Source Tungsten lamp Light Detector Silicon Photocell with narrow band inter-

Method Adaptation of the USEPA method 425.1 and

Environment 0 to 50°C (32 to 122°F); max 95% RH

Battery Type 1 x 9 volt Auto-Shut off After 10' of non-use in

Dimensions 192 x 102 x 67 mm

Weight 290 g (10 oz.).

ference filter @ 610 nm

Standard Methods for the Examination of Water and Wastewater, 20th edition, 5540C, Anionic Surfactants as MBAS.

non-condensing

after 1 hour of non-use in with last reading reminder.

(7.6 x 4 x 2.6")

measurement mode

calibration mode

;

Functional description

1) GLP/ key: press to enter edit the date and time.

2) CAL CHECK key: press to perform the validation of the meter, or press and hold for three seconds to enter the

3) ZERO/CFM key: press to zero the meter prior to measurement, to confirm edited values or to confirm factory calibration restore.

4) READ/ /TIMER key: In measurement, or press and hold for three seconds to start a preprogrammed countdown prior to measurement. In view the next screen.

5) ON/OFF key: to turn the meter on and off.

6) Liquid Crystal Display (LCD)

7) Cuvette alignment indicator

8) Cuvette holder

GLP mode

. In

calibration mode

measurement mode

press to

, press to make a

GLP mode

press to

DISPLAY ELEMENTS DESCRIPTION

1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or reading measurement

2) Error messages and warnings

3) The battery icon shows the charging level of the battery

4) The hourglass appears when an internal checking is in progress

5) Status messages

6) The chronometer appears when the reaction timer is running

7) The month, day and date icons appear when a date is displayed

8) Four digit main display

9) Measuring units

10) Four digit secondary display

Errors and warnings

ON ZERO READING

ON SAMPLE READING

Light High: There is too much light to perform a measurement. Please check the preparation of the zero cuvette.

Light Low: There is not enough light to perform a measurement. Please check the preparation of the zero cuvette.

No Light:The instrument cannot adjust the light level. Please check that the sample does not contain any debris.

Inverted cuvettes: The sample and the zero cuvette are inverted.

Zero: A zero reading was not taken. Follow the instructions of the measurement procedure for zeroing the meter.

Under range: A blinking “0.00” indicates that the sample absorbs less light than the zero reference. Check the procedure and make sure you use the same cuvette for reference (zero) and measurement.

Over Range: A flashing value of the maximum concentration indicates an over range condition. The concentration of the sample is beyond the programmed

DURING CALIBRATION PROCEDURE

range: dilute the sample and re-run the test.

Standard Low: The standard reading is less than expected.

Standard High: The standard reading is higher than expected.

OTHER ERRORS AND WARNINGS

Cap error: Appears when external light enters in the analysis cell. Assure that the cuvette cap is present.

Cooling lamp: The instrument waits for the lamp to cool down.

Battery low: The battery must be replaced soon.

Dead battery: This indicates that the battery is dead and must be replaced. Once this indication is displayed, the meter will lock up. Change the battery and restart the meter.

Measurement procedure

7, 13

8, 14

1• Turn the meter on by pressing ON/OFF. 2• When the beeper sounds briefly and the

LCD displays dashes, the meter is ready. The blinking “ZERO” indicates that the instrument needs to be zeroed first.

3• Fill the graduated glass vial with 25 mL of

sample. For most accurate results, use of a class A laboratory pipette is strongly recommended. Alternatively, it is possible to use the plastic pipette to fill the glass vial up to the 25 mL mark.

4• Add 2 drops of HI 95769A-0 Anionic

Surfactants Reagent A and 2 drops of HI 95769B-0 Anionic Surfactants Reagent B.

5• Close the glass vial with its cap and invert

to mix. The solution will turn blue.

6• Add exactly 10 mL of Chloroform reagent.

For most accurate results, use a class A glass laboratory pipette is strongly recommended. Alternatively, it is possible to use another clean plastic pipette to fill the vial up to the 10 mL mark. Note: Chloroform is heavier than water thus it will sink to the bottom of the graduated glass vial. Do not mix up the pipettes.

7• Close tightly the glass vial with its cap and

shake it vigorously for 30 seconds. Note: Block the cap with a finger during shaking.

8• Press and hold

3’’

seconds and the display will show the countdown. The beeper is playing a beep

READ/READ/

READ/

READ/READ/

/TIMER/TIMER

/TIMER for three

/TIMER/TIMER

at the end of countdown period. Alternatively, wait for 2 minutes leaving the vial capped and undisturbed.

11-12

Notes: The solution in the cuvette must be limpid.

If the solution is clouded, you can improve separation between the chloroform and aqueous layers by gently warming the cuvette (for instance by holding the cuvette in the hand). If the chloroform layer contains some aqueous drops hanging on the cuvette wall, gently swirl or invert the cuvette. It is important to transfer at least 7 mL of chloroform layer into the measurement cuvette, thus up to 0.5 cm (1/4”) below the 10 mL mark. If the transferred volume is lower than 7 mL, the accuracy of the test may be affected. Please repeat the test waiting for longer than 2 minutes, to allow complete separationbetween the two phases.

19, 23

During this period, the chloroform layer separates from the aqueous layer: the color of the aqueous layer will fade slightly if anionic surfactants are present, while the chloroform layer will turn blue.

9• Remove the cap. 10•Using the long plastic pipette, remove the

upper aqueous layer and discard. Do not remove the lower chloroform layer.

11•Add to the vial about 15 mL of deionized

water, up to the 25 mL mark.

12•Add 2 drops of HI 95769A-0 Anionic

Surfactants Reagent A.

13• Close tightly the glass vial with its cap and

shake it vigorously for 30 seconds. Note: Block the cap with a finger during shaking.

14•Press and hold

seconds and the display will show the countdown. The beeper is playing a beep at the end of countdown period. Alternatively, wait for 2 minutes leaving the vial capped and undisturbed. During this period, the chloroform layer separates from the aqueous layer.

15•Remove the cap. 16•Insert a

upper aqueous layer to transfer only the lower chloroform layer into a cuvette paying attention not to transfer the upper aqueous layer too.

17•Cap the cuvette. This is the reacted sample (#2). 18•Fill another cuvette with 10 mL of Chloroform

reagent up to the 10 mL mark and place the cap. This is the blank (#1).

19•Place the blank (cuvette #1) into the

cuvette holder and ensure that the notch on the cap is positioned securely into the groove.

20•Press ZERO/CFM and the lamp, cuvette

and detector icons will appear on the display, depending on the measurement phase.

21•After a few seconds, the display will show

“-0.0-”. The meter is now zeroed and

READ/READ/

READ/

READ/READ/

clean plastic pipette below the

/TIMER/TIMER

/TIMER for three

/TIMER/TIMER

22•Remove the cuvette. 23•Place the reacted sample (cuvette #2) into

the cuvette holder and ensure that the notch on the cap is positioned securely into the groove.

READ/READ/

READ/

READ/READ/

/TIMER/TIMER

. The lamp, cuvette

/TIMER

/TIMER/TIMER

24•Press

and detector icons will appear on the display, depending on the measurement phase.

25•At the end of measurement, the instrument

directly displays concentration in mg/L (ppm) of anionic surfactants (MBAS) as SDBD on the LCD.

20-21

CONVERSION FACTORS

The anionic detergent content is expressed as mg/L of SDBS. Convert the reading to mg/L (ppm) of a different anionic detergent concentration (DX) of known molecular weight (MWDX), as follows:

DX (mg/L) DX (mg/L)

DX (mg/L)

DX (mg/L) DX (mg/L)

INTERFERENCES

Cationic surfactants negative interference Absorption particulate matter negative interference Sulfide negative interference Organic sulfates, sulfonates positive interference Strong oxidants (Cl2, H2O2, S2O Highly buffered samples or with extreme pH may exceed the buffering capacity of the reagent: pH should be adjusted between 4 and 9 with diluted NaOH for acidic samples or with diluted HCl for alkaline samples, prior to addition of the reagent.

Reading (mg/L SDBS) x MW

348.48

, etc.) negative interference

Validation and Calibration procedures

Warning: do not validate or calibrate the instrument with standard solutions other than the Hanna CAL CHECK™ Standards, otherwise erroneous results will be obtained.

Use the Hanna CAL CHECK™ cuvettes (see “Accessories”) to validate or calibrate instruments.

VALIDATION

1• Turn the meter on by pressing ON/OFF. 2• When the beeper sounds briefly and the

LCD displays dashes, the meter is ready.

3• Place the CAL CHECK™ Standard

HI 96769-11 Cuvette A into the cuvette

holder and ensure that the notch on the cap is positioned securely into the groove.

4• Press ZERO/CFM and the lamp, cuvette

and detector icons will appear on the display, depending on the measurement phase.

5• After a few seconds the display will show

“-0.0-”. The meter is now zeroed and ready for validation.

6• Remove the cuvette. 7• Place the CAL CHECK™ Standard

Validation

4-5

HI 96769-11 Cuvette B into the cuvette holder and ensure that the notch on the cap is positioned securely into the groove.

8• Press CAL CHECK key and the lamp,

cuvette and detector icons together with “CAL CHECK” will appear on the display, depending on the measurement phase.

9• At the end of the measurement the display

will show the validation standard value. The reading should be within specifications as reported on the CAL CHECK™ Standard Certificate. If the value is found out of specifications, please check that the cuvettes are free of fingerprints, oil or dirt and repeat validation. If results are still found out of specifications then recalibrate the instrument.

CALIBRATION

Note: It is possible to interrupt the calibration

procedure at any time by pressing CAL CHECK or ON/OFF keys.

1• Turn the meter on by pressing ON/OFF. 2• When the beeper sounds briefly and the

LCD displays dashes, the meter is ready.

3• Press and hold CAL CHECK for three

seconds to enter display will show “CAL” during calibration procedure. The blinking “ZERO” asks for instrument zeroing.

4• Place the CAL CHECK™ Standard HI

96769-11 Cuvette A into the cuvette holder

and ensure that the notch on the cap is positioned securely into the groove.

6• Press ZERO/CFM and the lamp, cuvette

and detector icons will appear on the display, depending on the measurement phase.

7• After a few seconds the display will show

“-0.0-”. The meter is now zeroed and ready for calibration. The blinking “READ” asks for reading calibration standard.

8• Remove the cuvette. 9• Place the CAL CHECK™ Standard

HI 96769-11 Cuvette B into the cuvette holder and ensure that the notch on the cap is positioned securely into the groove.

10•Press READ/ /TIMER and the lamp,

cuvette and detector icons will appear on the display, depending on the measurement phase.

11•The instrument will show for three seconds

the CAL CHECK™ standard value.

Note: If the display shows “STD HIGH”, the

standard value was too high. If the display shows “STD LOW”, the standard value was too low. Verify that both CAL CHECK™ Standard HI 96769-11 Cu-

calibration mode.

The

vettes, A and B are free from fingerprints

Calibration

6-7

10-11

or dirt and that they are inserted correctly.

Then the date of last calibration (e.g.: “01.08.2005”) appears on the display, or “01.01.2005” if the factory calibration was selected before. In both cases the year number is blinking, ready for date input.

12•Press GLP/ to edit the desired year

(2000-2099). If the key is kept pressed, the year number is automatically increased.

13•When the correct year has been set, press

ZERO/CFM or READ/ /TIMER to confirm. Now the display will show the month blinking.

14•Press GLP/ to edit the desired month

(01-12). If the key is kept pressed, the month number is automatically increased.

15•When the correct month has been set,

press ZERO/CFM or READ/ /TIMER to confirm. Now the display will show the day blinking.

16•Press GLP/ to edit the desired day (01-

31). If the key is kept pressed, the day number is automatically increased.

Note: It is possible to change the editing from

day to year and to month by pressing

READ/ /TIMER.

17• Press ZERO/CFM to save the calibration date. 18•The instrument displays “Stor” for one

second and the calibration is saved.

19•The instrument will return automatically

measurement mode

on the LCD.

by displaying dashes

GLP

In the

GLP mode

factory calibration can be restored.

LAST CALIBRATION DATE

1• Press GLP/ to enter

2• If no calibration was performed, the factory

FACTORY CALIBRATION RESTORE

It is possible to delete the calibration and restore factory calibration.

1• Press GLP/ to enter

, the last calibration date can be consulted and the

GLP mode

calibration month and day will appear on the main display and the year on the secondary display.

calibration message, “F.CAL” will appear on the main display and the instrument returns to

measurement mode

. The

after three

seconds.

GLP mode

12-13

14-15

17-18

Last

Calibration

Date

3-4

Factory

Calibration

Restore

2• Press READ/ /TIMER to enter in the

factory calibration restore screen. The instrument asks for confirmation of user calibration delete.

3• Press ZERO/CFM to restore the factory

calibration or press GLP/ again to abort factory calibration restore.

4• The instrument briefly notifies “done” when

restores factory calibration and returns to

measurement mode

Battery management

To save battery, the instrument shuts down after 10 minutes of non-use in

measurement mode

If a valid measurement was displayed before auto-shut off, the value is displayed when the instrument is switched on. The blinking “ZERO” means that a new zero has to be performed. One fresh battery lasts for around 750 measurements, depending on the light level. The remaining battery capacity is evaluated at the instrument startup and after each measurement. The instrument displays a battery indicator with three levels as follows:

• 3 lines for 100 % capacity

• 2 lines for 66 % capacity

• 1 line for 33 % capacity

• Battery icon blinking if the capacity is under 10 %. If the battery is empty and accurate measurements can’t be taken any more, the instrument shows “ To restart the instrument, the battery must be replaced with a fresh one. To replace the instrument’s battery, follow the steps:

• Turn the instrument off by pressing ON/OFF.

• Turn the instrument upside down and remove the battery cover by turning it counterclockwise.

• Extract the battery from its location and replace it with a fresh one.

• Insert back the battery cover and turn it clockwise to close.

and after 1 hour of non-use in

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dead batt” and turns off.

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calibration mode.

Accessories

REAGENT SETREAGENT SET

REAGENT SET

REAGENT SETREAGENT SET HI 95769-01 Reagents for 40 Anionic Surfactants tests

OTHER ACCESORIESOTHER ACCESORIES

OTHER ACCESORIES

OTHER ACCESORIESOTHER ACCESORIES HI 96769-11 CAL CHECK

Standard Cuvettes (1 set)

HI 70000P 20 mL Deionized rinse solution (25 pcs)

HI 740220 25 mL glass test tubes with caps (2 pcs) HI 721310 9V battery (10 pcs.) HI 731318 Cloth for wiping cuvettes (4 pcs.) HI 731331 Glass cuvettes (4 pcs.) HI 731335 Caps for cuvettes HI 93703-50 Cuvette cleaning solution (230 mL)

Warranty

HI 96769 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure. If the repair is not covered by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization Number from the Customer Service Department and then send it with shipment costs prepaid. When shipping any instrument, make sure it is properly packaged for complete protection. To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of purchase.

Recommendations for Users

Before using these products, make sure that they are entirely suitable for your specific application and for the environment in which they are used. Operation of these instruments may cause unacceptable interferences to other electronic equipments, this requiring the operator to take all necessary steps to correct interferences. Any variation introduced by the user to the supplied equipment may degrade the instrument’s EMC performance. To avoid damages or burns, do not put the instrument in microwave oven. For yours and the instrument safety do not use or store the instrument in hazardous environments.

Hanna Instruments reserves the right to modify the

design, construction and appearance of its products

without advance notice.

For additional information, contact your

dealer or the nearest

Hanna Customer Service Center.

To find the Hanna Office in your area,

IST96769 01/11

visit our web site

www.hannainst.com