Hanna Instruments HI 96765 User Manual
Instruction Manual
HI 96765
Blood Plasma Analyzer
www.hannainst.com
1
Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION ..............................................................................................3
GENERAL DESCRIPTION .....................................................................................................4
ABBREVIATIONS ................................................................................................................4
SPECIFICATIONS ................................................................................................................. 4
PRINCIPLE OF OPERATION .................................................................................................5
FUNCTIONAL DESCRIPTION ................................................................................................ 6
ERRORS AND WARNINGS ..................................................................................................8
GENERAL TIPS FOR AN ACCURATE MEASUREMENT ........................................................... 10
STARTUP ..................................................................................................................10
MEASUREMENT PROCEDURE ...........................................................................................11
BATTERY MANAGEMENT .................................................................................................. 13
BATTERY REPLACEMENT ..................................................................................................14
ACCESSORIES .................................................................................................................14
WARRANTY .................................................................................................................15
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
2
PRELIMINARY EXAMINATION
Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occured during shipment, please notify your Dealer.
Each HI 96765 Blood Plasma Analyzer is supplied complete with:
• Light Blocking Cap
• 9V Battery
• Cloth for wiping tubes
• Instrument quality certificate
• Instruction Manual
• Rigid carrying case
Note:Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.
3
GENERAL DESCRIPTION
The HI 96765 meter measures the light absorbance of animal blood plasma: the absorbance
value allows identification of the plasma quality.
Display codes aid the user in routine operations.
The meters have an auto-shut off feature that will turn the instrument off after 10 minutes of non-use.
This instrument has been developed to measure centrifuged animal blood, coming from abattoirs.
The quality of the blood is checked by measuring its color, expressed in Quality Units. When the
blood cells are damaged, haemoglobin is found in the plasma, which causes high readings. The
lower are the readings in Quality Units (less color), the higher is the blood plasma quality.
This Hanna specialty meter reads directly the blood plasma quality from 0 to 10 units.
ABBREVIATIONS
degree Celsius
°C:
degree Fahrenheit
°F:
milliliter
mL:
mV:
millivolts
SPECIFICATIONS
Range 0.0 to 10.0 units
Resolution 0.1 unit
Accuracy ±0.2 units or 3% of reading @ 25°C (whichever is greater)
Typical EMC Deviation ±0.1
Light Source Light emitting diode @ 555 nm
Light Detector Silicon Photocell
Method Direct Measurement
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 1 x 9 volt
Auto-Shut off After 10' of non-use
Dimensions 192 x 102 x 67 mm (7.6 x 4 x 2.6")
Weight 290 g (10 oz.).
REQUIRED REAGENTS
Description Quantity/test
Blood Animal 1.5 mL
4
PRINCIPLE OF OPERATION
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by
atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law:
-log I/Io = ελ c d
or
A = ελ c d
Where:
-log I/I
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound
from a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna's HI 96 series colorimeters is based on special subminiature
tungsten lamps and narrow-band interference filters to guarantee both high performance and
reliable results.
= Absorbance (A)
o
Io= intensity of incident light beam
I = intensity of light beam after absorption
ελ= molar extinction coefficient at wavelength λ
c = molar concentration of the substance
d = optical path through the substance
HI 96 series block diagram (optical layout)
5
Loading...