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HI 96715
User Manual
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Hanna Instruments HI 96715 User Manual

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Instruction Manual
HI 96715C
Ammonia Medium Range ISM
www.hannainst.com
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Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION ................................................................................................ 3
GENERAL DESCRIPTION ....................................................................................................... 4
ABBREVIATIONS .................................................................................................................. 4
SPECIFICATIONS ................................................................................................................... 5
PRECISION AND ACCURACY .................................................................................................. 5
PRINCIPLE OF OPERATION ................................................................................................... 6
FUNCTIONAL DESCRIPTION .................................................................................................. 7
ERRORS AND WARNINGS .................................................................................................... 9
PRELIMINARY EXAMINATION
Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your Dealer.
Each HI 96715 Ion Selective Meter is supplied complete with:
• Two Sample Cuvettes and Caps
• Two CAL CHECKTM standard cuvettes HI 96715KIT
• 9V Battery
• Tissue for wiping cuvettes
• Instrument quality certificate
• Instruction Manual
• Rigid carrying case
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
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Note:Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.
GENERAL DESCRIPTION
SPECIFICATIONS
The HI 96715 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s years of experience as a manufacturer of analytical instruments. It has the advanced optical system based on a special tungsten lamp and a narrow band interference filter that allows most accurate and repeatable readings. All instruments are factory calibrated and the electronic and optical design minimizes the need of frequent calibration.
With the powerful CAL CHECKTM validation function, you are able to validate good performance of your instrument at any time. The validation procedure is extremely user friendly. Just use the exclusive HANNA ready-made, NIST traceable standards to verify the performance of the instrument and recalibrate if necessary.
The instrument is splash waterproof and the LED and filter units are protected from dust or dirt by a transparent cup. This makes the instruments fulfill field applications. Display messages aid the user in routine operation. The meter has an auto-shut off feature that will turn off the instrument after 10 minutes of non use in
calibration mode
The meter uses an exclusive positive-locking system to ensure that the cuvette is in the same position every time it is placed into the measurement cell. It is designed to fit a cuvette with a larger neck making it easier to add both sample and reagents. The cuvette is made from special optical glass to obtain best results.
The HI 96715 meter measures the ammonia nitrogen (NH3-N) content in water samples in the
0.00 to 9.99 mg/L (ppm) range. The method is an adaptation from ASTM Manual of Water and Environmental Technology, D1426-93, Nessler Method.
The reagents are liquid form and are supplied in bottles. The amount of reagent is precisely dosed to ensure the maximum repeatability.
.
measurement mode
or after 1 hour if left in
ABBREVIATIONS
degree Celsius
°C:
degree Fahrenheit
°F:
milligrams per liter. mg/L is equivalent to ppm (parts per million)
mg/L:
milliliter
mL:
Light Emitting Diode
LED:
Liquid Crystal Display
LCD:
American Society for Testing and Materilas
ASTM:
Range 0.00 to 9.99 mg/L (as NH3-N) Resolution 0.01 mg/L for
0.1 mg/L for
Accuracy ±0.05 mg/L ±5% of reading @ 25°C Typical EMC Deviation ±0.01 mg/L Light Source Light Emitting Diode Light Detector Silicon Photocell with narrow band interference filter @ 466 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426-93, Nessler Method. The reaction between ammonia and the reagents causes a yellow tint in the sample.
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing Battery Type 1 x 9 volt Auto-Shut off After 10’ of non-use in
after 1 hour of non-use in
with last reading reminder.
Dimensions 192 x 104 x 69 mm (7.6 x 4.1 x 2.7”) Weight 360 g (12.7 oz.).
REQUIRED REAGENTS Code Description Quantity/test HI 93715A-0 First Reagent 4 drops (6 drops in seawater) HI 93715B-0 Second Reagent 4 drops (10 drops in seawater)
measurement
calibration and validation
measurement mode
calibration mode
;
;
PRECISION AND ACCURACY
Precision is how closely repeated measurements agree with each other. Precision is usually expressed as standard deviation (SD).
Accuracy is defined as the nearness of a test result to the true value.
Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these definitions.
In a laboratory using a standard solution of 6.00 mg/L ammonia nitrogen (NH3-N) and a representative lot of reagents, an operator obtained with a single instrument a standard deviation of 0.12 mg/L.
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PRINCIPLE OF OPERATION
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical-chemical characteristics of the substance according to the Lambert-Beer Law:
-log I/Io = ελ c d
or
A = ελ c d
Where:
-log I/I
= Absorbance (A)
o
Io= intensity of incident light beam I = intensity of light beam after absorption
ελ= molar extinction coefficient at wavelength λ
c = molar concentration of the substance d = optical path through the substance
A microprocessor controlled LED emits radiation which is first optically conditioned and beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of intensity Io or I.
The photoelectric cell collects the radiation I that is not absorbed by the sample and converts it into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed.
The cuvette has a very important role because it is an optical element and thus requires particular attention. It is important that both, the measurement and the calibration (zeroing) cuvettes, are optically identical to provide the same measurement conditions. Whenever possible use the same cuvette for both. It is necessary that the surface of the cuvette is clean and not scratched. This to avoid measurement interference due to unwanted reflection and absorption of light. It is recommended not to touch the cuvette walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring phases, it is necessary to close the cuvette to prevent any contamination.
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound from a specific chemical reaction between sample and reagents. Given that the absorption of a compound strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna's HI 96715 colorimeter is based on a Light Emitting Diode (LED) combined with a narrow-band interference filter to guarantee both high performance and reliable results.
HI 96715 block diagram (optical layout)
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FUNCTIONAL DESCRIPTION
INSTRUMENT DESCRIPTION
1) GLP/ key
2) CAL CHECK key
3) ZERO/CFM key
4) READ/ /TIMER key
5) ON/OFF key
6) Liquid Crystal Display (LCD)
7) Cuvette alignment indicator
8) Cuvette holder
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8
5
2
9
7
3
4
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KEYPAD DESCRIPTION
• ON/OFF: to turn the meter on and off.
• ZERO/CFM: this is a bi-functional key. Just press to zero the meter prior to measurement, or to confirm edited values. In or to confirm factory calibration restore.
• READ/ /TIMER: this is a multi-functional key. In
measurement mode
, press to make a measurement, or press and hold for three seconds to start a pre-programmed countdown prior to measurement. In
GLP mode
press to view the next screen.
• CAL CHECK: this is a bi-functional key. Just press to perform the validation of the meter, or press and hold for three seconds to enter
• GLP/ : this is a bi-functional key. Just press to enter
calibration mode
GLP mode
.
. In
calibration mode
press
to edit the date and time.
OPERATING MODES
•
Measurement mode:
•
Calibration mode:
default operation mode, enables both validation and measurement.
may be entered by keeping CAL CHECK pressed for three seconds (the
“CAL” tag appears), it enables calibration of the instrument.
•
GLP mode
may be entered by pressing GLP/ (“GLP” appears), it enables consulting of
user calibration date or restore factory calibration.
ERRORS AND WARNINGS
The instrument shows clear messages when erroneous condition appears. Messages are also displayed when the obtained values are outside expected range. The beeper is playing a beep on errors.
a) on zero reading
Light High: There is too much light to perform a measurement. Please check the preparation of the zero cuvette.
Light Low: There is not enough light to perform a measurement. Please check the preparation of the zero cuvette.
DISPLAY ELEMENTS DESCRIPTION
check that the sample does not contain any debris.
b) on sample reading
Inverted cuvettes: The sample and the zero cuvette are inverted.
1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or
reading measurement
2) Error messages and warnings
3) The battery icon shows the charging level of the battery
No Light:The instrument cannot adjust the light level. Please
4) The hourglass appears when an internal checking is in progress
5) Status messages
6) The chronometer appears when the reaction timer is running
Zero: A zero reading was not taken. Follow the instructions of the measurement procedure for zeroing the meter.
7) The month, day and date icons appear when a date is displayed
8) Four digit main display
9) Measuring units
10) Four digit secondary display
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Under range: A blinking “0.00” indicates that the sample absorbs less light than the zero reference. Check the procedure and make sure you use the same cuvette for reference (zero) and measurement.
Battery low: The battery must be replaced soon.
c) during calibration procedure
d) other errors and warnings
Over Range: A flashing value of the maximum concentration indicates an over range condition. The concentration of the sample is beyond the programmed range: dilute the sample and re-run the test.
Standard Low: The standard reading is less than expected.
Standard High: The standard reading is higher than expected.
Cap error: Appears when external light enters in the analysis
cell. Assure that the cuvette cap is present.
Dead battery: This indicates that the battery is dead and must be replaced. Once this indication is displayed, the meter will lock up. Change the battery and restart the meter.
GENERAL TIPS FOR AN ACCURATE MEASUREMENT
The instructions listed below should be carefully followed during testing to ensure best accuracy.
• Color or suspended matter in large amounts may cause interference, therefore these should be removed by treatment with active carbon and by prior filtration.
• For a correct filling of the cuvette: the liquid in the cuvette forms a concavity on the top; the bottom of this concavity must be at the same level of the 10 mL mark.
• Proper use of dropper: (a) to get good reproducible results, tap the dropper on the table for several times and wipe
the outside of the dropper with a cloth.
(b) always keep the dropper bottle in a vertical position while dosing the reagent.
Cooling lamp: The instrument waits for the lamp to cool down.
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(a) (b)
• It is important that the sample does not contain any debris. This would corrupt the reading.
• In order to avoid reagent leaking and to obtain more accurate measurements, it is recommended to close the cuvette first with the supplied HPDE plastic stopper and then with the black cap.
• Each time the cuvette is used, the cap must be tightened to the same degree.
STARTUP
Prepare the instrument for measurement as follows:
• Unpack the instrument by removing the dust protection sleeve from the instrument cuvette holder.
• Place the battery in the instrument as described in the “BATTERY REPLACEMENT” chapter.
• Place the instrument on a flat table.
• Do not place the instrument under direct sun light.
MEASUREMENT PROCEDURE
• Whenever the cuvette is placed into the measurement cell, it must be dry outside, and completely free of fingerprints, oil or dirt. Wipe it thoroughly with HI 731318 or a lint-free cloth prior to insertion.
• Shaking the cuvette can generate bubbles in the sample, causing higher readings. To obtain accurate measurements, remove such bubbles by swirling or by gently tapping the cuvette.
• Do not let the reacted sample stand too long after reagent is added, or accuracy will be lost.
• It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for each sample and to use the same cuvette for zeroing and measurement.
• After the reading it is important to discard immediately the sample, otherwise the glass might become permanently stained.
• All the reaction times reported in this manual are referred to 20°C (68°F). As a general rule of thumb, they should be doubled at 10°C (50°F) and halved at 30°C (86°F).
• In order to maximize accuracy, prior to a measurement follow the validation procedure to be sure that the instrument is properly calibrated. If necessary, calibrate the instrument.
To compensate the meter for the sample turbidity or color, the measurement takes place in two phases. First, the meter is zeroed using the unreacted sample. After the reagents are added the reacted sample is measured.
• Turn the meter on by pressing The display briefly shows all tags on.
• When the beeper sounds briefly and the LCD displays dashes, the meter is ready. The blinking “ZERO” indicates that the instrument needs to be zeroed first.
• Fill the cuvette with 10 mL of unreacted sample, up to the mark, and replace the cap.
ON/OFFON/OFF
ON/OFF.
ON/OFFON/OFF
• Place the cuvette into the cuvette holder and ensure that the notch on the cap is positioned securely into the groove.
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• Press ZERO/CFM and the lamp, cuvette and detector icons will appear on the display, depending on the measurement phase.
• After a few seconds, the display will show “-0.0-”. The meter is now zeroed and ready for measurement.
Alternatively, wait for 3 minutes and 30
READ/READ/
seconds and just press In both cases, the lamp, cuvette and detector icons will appear on the display, depending on the measurement phase.
• At the end of measurement, the instrument directly displays concentration in mg/L of ammonia nitrogen (NH3-N) on the LCD.
READ/
READ/READ/
/TIMER/TIMER
/TIMER.
/TIMER/TIMER
• Remove the cuvette, open it and add 4 drops of HI 93715A-0 First Reagent (6 drops in case of seawater analysis). Swirl the cuvette gently to mix.
• Add 4 drops of HI 93715B-0 Second Reagent to the cuvette (10 drops in case of seawater analysis). Replace the cap and swirl the cuvette to mix.
• Replace the cuvette into the cuvette holder and ensure that the notch on the cap is positioned securely into the groove.
READ/READ/
• Press and hold
seconds. The display will show the
countdown prior to measurement. The beeper is playing a beep at the end of countdown period.
READ/
READ/READ/
/TIMER/TIMER
/TIMER for three
/TIMER/TIMER
• To convert the reading to mg/L of ammonia (NH3), multiply by a factor of
1.216.
INTERFERENCES
• Organic compounds like: chloramines, various aliphatic and aromatic amines, glycine,
(positive error). To eliminate these interferences distillation is required.
• Organic compounds like: aldehydes, alcohols (e.g. ethanol) or acetone (negative error).
To eliminate these interferences distillation is required.
• Sulfide (S2-): may cause turbidity.
• Hardness above 1 g/L as Calcium Carbonate (CaCO3).
VALIDATION PROCEDURE
Use the validation procedure to ensure that the instrument is properly calibrated.
Warning: Do not validate the instrument with any standard solutions other than the HANNA CAL CHECKTM Standards, otherwise erroneous results will be obtained. For accurate validation, please
perform test at room temperature: 18 to 25°C (64.5 to 77.0°F)
• Turn the meter on by pressing ON/OFF.
• When the beeper sounds briefly and the LCD displays dashes, the meter is ready.
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• Place the CAL CHECKTM Standard HI 96715-11 Cuvette A into the cuvette holder and ensure that the notch on the cap is positioned securely into the groove.
• Press ZERO/CFM and the lamp, cuvette and detector icons will appear on the display, depending on the measurement phase.
CALIBRATION PROCEDURE
Note: It is possible to interrupt calibration procedure at any time by pressing CAL CHECK or ON/OFF keys. Warning: Do not calibrate the instrument with standard solutions other than the HANNA CAL CHECKTM Standards, otherwise erroneous results will be obtained. For accurate calibration, please
perform test at room temperature: 18 to 25°C (64.5 to 77.0°F)
• Turn the meter on by pressing ON/OFF.
• After a few seconds, the display will show “-0.0-”. The meter is now zeroed and ready
for validation.
• Remove the cuvette.
• Place the CAL CHECKTM Standard HI 96715-11 Cuvette B into the cuvette holder and ensure
that the notch on the cap is positioned securely into the groove.
• Press CAL CHECKTM and the lamp, cuvette and detector icons together with “CAL CHECK” will appear on the display, depending on the measurement phase.
• At the end of the measurement the display will show the validation standard value.
The reading should be within specifications as reported in the CAL CHECKTM Standard Certificate. If the value is found out of the specifications, please check that the cuvettes are free of fingerprints, oil or dirt and repeat validation. If results are still found out of specifications, then recalibrate the instrument.
• When the beeper sounds briefly and the LCD displays dashes, the meter is ready.
• Press and hold CAL CHECK for three
seconds to enter
display will show “CAL” during calibration procedure. The blinking “ZERO” asks for instrument zeroing.
• Place the CAL CHECKTM Standard HI 96715-11 Cuvette A into the cuvette holder and ensure that the notch on the cap is positioned securely into the groove.
• Press ZERO/CFM and the lamp, cuvette and detector icons will appear on the display, depending on the measurement phase.
calibration mode
. The
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• After a few seconds the display will show “-0.0-”. The meter is now zeroed and ready
for calibration. The blinking “READ” asks for reading calibration standard.
• Remove the cuvette.
• Place the CAL CHECKTM Standard HI 96715-11 Cuvette B into the cuvette holder and ensure that the notch on the cap is positioned securely into the groove.
DATE INPUT
• Press GLP/ to edit the desired year
(2000-2099). If the key is kept pressed, the year number is automatically increased.
• When the correct year has been set, press ZERO/CFM or READ/ /TIMER to confirm. Now the display will show the month blinking.
• Press GLP/ to edit the desired month
(01-12). If the key is kept pressed, the month number is automatically increased.
or
• Press READ/ /TIMER and the lamp, cuvette and detector icons will appear on the display, depending on the measurement phase.
• After measurement the instrument will show for three seconds the Cal Check Standard value.
Note: If the display shows “STD HIGH”, the
standard value was too high. If the display shows “STD LOW”, the standard value was too low. Verify that both CAL CHECK Standards HI 96715-11 Cuvettes, A and B are free from fingerprints or dirt and that they are inserted correctly.
Then the date of the last calibration (e.g.:
”01.08.2005”) appears on the display, or “01.01.2005” if the factory calibration was
selected before. In both cases the year number is blinking, ready for date input.
or
• When the correct month has been set, press ZERO/CFM or READ/ /TIMER to confirm. Now the display will show the day blinking.
• Press GLP/ to edit the desired day
(01-31). If the key is kept pressed, the day number is automatically increased.
TM
Note: It is possible to change the editing
from day to year and to month by pressing READ/ /TIMER.
• Press ZERO/CFM to save the calibration date.
• The instrument displays “Stor” for one second and the calibration is saved.
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• The instrument will return automatically to the measurement mode by displaying dashes on the LCD.
GLP
• Press READ/ /TIMER to enter in the factory calibration restore screen. The instrument asks for confirmation of user calibration delete.
• Press ZERO/CFM to restore the factory
calibration or press GLP/ again to
abort factory calibration restore.
In the GLP mode, the last user calibration date can be consulted and the factory calibration can be restored.
LAST CALIBRATION DATE
To display the calibration date:
• Press GLP/ to enter
calibration month and day will appear on the main display and the year on the secondary display.
• If no calibration was performed, the factory calibration message, “F.CAL” will appear on the main display and the instrument returns to after three seconds.
FACTORY CALIBRATION RESTORE
It is possible to delete the calibration and restore factory calibration.
• Press GLP/ to enter
GLP mode
. The
measurement mode
GLP mode
.
• The instrument briefly notifies “done” when restores factory calibration and returns to
measurement mode
.
BATTERY MANAGEMENT
To save battery, the instrument shuts down after 10 minutes of non-use in and after 1 hour of non-use in
If a valid measurement was displayed before auto shut off, the value is displayed when the instrument is switched on. The blinking “ZERO” means that a new zero has to be performed.
One fresh battery lasts for around 750 measurements, depending on the light level. The remaining battery capacity is evaluated at the instrument startup and after each measurement. The instrument displays a battery indicator with three levels as follows:
• 3 lines for 100 % capacity
• 2 lines for 66 % capacity
• 1 line for 33 % capacity
• Empty battery icon blinking if the capacity is under 10 %.
If the battery is empty and accurate measurements can’t be taken anymore, the instrument
dead battdead batt
shows “
dead batt” and turns off.
dead battdead batt
To restart the instrument, the battery must be replaced with a fresh one.
calibration mode.
measurement mode
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BATTERY REPLACEMENT
WARRANTY
To replace the instrument’s battery, follow the steps:
• Turn the instrument off by pressing ON/OFF.
• Turn the instrument upside down and remove the battery cover by turning it counterclockwise.
• Extract the battery from its location and replace it with a fresh one.
• Insert back the battery cover and turn it clockwise to close.
ACCESORIES
REAGENT SETREAGENT SET
REAGENT SET
REAGENT SETREAGENT SET HI 93715-01 Reagents for 100 tests HI 93715-03 Reagents for 300 tests
HI 96715 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions.
This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair is not covered by the warranty, you will be notified of the charges incurred.
If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization Number from the Customer Service Department and then send it with shipment costs prepaid. When shipping any instrument, make sure it is properly packaged for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of purchase.
OTHER ACCESORIESOTHER ACCESORIES
OTHER ACCESORIES
OTHER ACCESORIESOTHER ACCESORIES HI 96715-11 CAL CHECKTM Standard Cuvettes (1 set) HI 721310 9V battery (10 pcs.) HI 731318 Tissue for wiping cuvettes (4 pcs.) HI 731331 Glass cuvettes (4 pcs.) HI 731335 Caps for cuvettes HI 93703-50 Cuvettes cleaning solution (230 mL)
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Recommendations for Users
Before using these products, make sure that they are entirely suitable for your specific application and for the environment in which they are used.
Operation of these instruments may cause unacceptable interferences to other electronic equipments, this requiring the operator to take all necessary steps to correct interferences.
Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC performance. To avoid damages or burns, do not put the instrument in microwave oven. For yours and the instrument safety do not use or
store the instrument in hazardous environments.
Hanna Instruments reserves the right to modify the design, construction and appearance of its products without advance notice.
Hanna Instruments Inc.
Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA
Technical Support for Customers
Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.com
Local Sales and Customer Service Office
Printed in EUROPE
(ROMANIA)
MAN96715 07/10
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