Hanna Instruments HI 96713 User Manual

Instruction Manual

HI 96713C

Phosphate Low Range ISM

www.hannainst.com

Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary

information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ................................................................................................ 3

GENERAL DESCRIPTION ....................................................................................................... 4

ABBREVIATIONS .................................................................................................................. 4

SPECIFICATIONS ................................................................................................................... 5

PRECISION AND ACCURACY .................................................................................................. 5

PRINCIPLE OF OPERATION ................................................................................................... 6

FUNCTIONAL DESCRIPTION .................................................................................................. 7

ERRORS AND WARNINGS .................................................................................................... 9

GENERAL TIPS FOR AN ACCURATE MEASUREMENT ............................................................. 11

STARTUP ........................................................................................................................... 12

MEASUREMENT PROCEDURE ............................................................................................. 12

VALIDATION PROCEDURE ................................................................................................... 15

CALIBRATION PROCEDURE ................................................................................................. 17

GLP ................................................................................................................................... 20

BATTERY MANAGEMENT .................................................................................................... 21

BATTERY REPLACEMENT .................................................................................................... 22

ACCESSORIES ..................................................................................................................... 22

WARRANTY........................................................................................................................ 23

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your Dealer.

Each HI 96713 Ion Selective Meter is supplied complete with:

• Two Sample Cuvettes and Caps

• CAL CHECK standard cuvettes

• 9V Battery

• Cloth for wiping cuvettes

• Instrument quality certificate

• Instruction Manual

• Rigid carrying case

Note: Save all packing material until you are sure that the instrument works correctly. Any

defective item must be returned in its original packing.

GENERAL DESCRIPTION

The HI 96713 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s years of experience as a manufacturer of analytical instruments. It has the advanced optical system based on a special Tungsten Lamp and a narrow band interference filter that allows most accurate and repeatable readings. All instruments are factory calibrated and the electronic and optical design minimizes the need of frequent calibration.

With the powerful CAL CHECKTM validation function, you are able to validate good performance of your instrument at any time. The validation procedure is extremely user friendly. Just use the exclusive HANNA ready-made, NIST traceable standards to verify the performance of the instrument and recalibrate if necessary.

All instruments are splash waterproof and the lamp and filter units are protected from dust or dirt by a transparent cup. This makes the instruments fulfill field applications. Display messages aid the user in routine operation. The meter has an auto-shut off feature that will turn off the instrument after 10 minutes of non use in

calibration mode

The meter uses an exclusive positive-locking system to ensure that the cuvette is in the same position every time it is placed into the measurement cell. It is designed to fit a cuvette with a larger neck making it easier to add both sample and reagents. The cuvette is made from special optical glass to obtain best results.

The HI 96713 meter measures the phosphate content in water samples in the 0.00 to 2.50 mg/L (ppm) range. The method is an adaptation of the ascorbic acid method.

The reagent is in powder form and is supplied in packets. The amount of reagent is precisely dosed to ensure the maximum repeatability.

measurement mode

or after 1 hour if left in

ABBREVIATIONS

degree Celsius

°C:

degree Fahrenheit

°F:

micrograms per liter. mg/L is equivalent to ppm (parts per million)

mg/L:

milliliter

mL:

millivolts

mV:

SPECIFICATIONS

Range 0.00 to 2.50 mg/L Resolution 0.01 mg/L Accuracy ±0.04 mg/L ± 4% of reading @ 25°C Typical EMC Deviation ±0.01 mg/L Light Source Tungsten lamp Light Detector Silicon Photocell with narrow band interference filter @ 610 nm Method Adaptation of the ascorbic acid method. The reaction between

phosphate and the reagent causes a blue tint in the sample.

Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing Battery Type 1 x 9 volt Auto-Shut off After 10' of non-use in

after 1 hour of non-use in with last reading reminder.

Dimensions 192 x 104 x 69 mm (7.6 x 4.1 x 2.7") Weight 360 g (12.7 oz.).

REQUIRED REAGENTS Code Description Quantity/test HI 93713-0 Powder Reagent 1 packet

measurement mode

calibration mode

;

PRECISION AND ACCURACY

Precision is how closely repeated measurements agree with each other. Precision is usually expressed as standard deviation (SD).

Accuracy is defined as the nearness of a test result to the true value.

Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these definitions.

In a laboratory using a standard solution of

1.00 mg/L phosphate and a representative lot of reagent, an operator obtained with a single instrument a standard deviation of 0.03 mg/L.

PRINCIPLE OF OPERATION

Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.

If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical-chemical characteristics of the substance according to the Lambert-Beer Law:

-log I/Io = ελ c d

A = ελ c d

Where:

-log I/I

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are known.

Photometric chemical analysis is based on the possibility to develop an absorbing compound from a specific chemical reaction between sample and reagents. Given that the absorption of a compound strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should be selected as well as a proper central wavelength to optimize measurements.

The optical system of Hanna's HI 96 colorimeter is based on special tungsten lamp and narrow-band interference filters to guarantee both high performance and reliable results.

= Absorbance (A)

Io= intensity of incident light beam I = intensity of light beam after absorption

ελ= molar extinction coefficient at wavelength λ

c = molar concentration of the substance d = optical path through the substance

HI 96 block diagram (optical layout)

A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned and beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of intensity Io or I.

The photoelectric cell collects the radiation I that is not absorbed by the sample and converts it into an electric current, producing a potential in the mV range.

The microprocessor uses this potential to convert the incoming value into the desired measuring unit and to display it on the LCD.

The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed.

The cuvette has a very important role because it is an optical element and thus requires particular attention. It is important that both, the measurement and the calibration (zeroing) cuvettes, are optically identical to provide the same measurement conditions. Whenever possible use the same cuvette for both. It is necessary that the surface of the cuvette is clean and not scratched. This to avoid measurement interference due to unwanted reflection and absorption of light. It is recommended not to touch the cuvette walls with hands.

Furthermore, in order to maintain the same conditions during the zeroing and the measuring phases, it is necessary to close the cuvette to prevent any contamination.

FUNCTIONAL DESCRIPTION

INSTRUMENT DESCRIPTION

1) GLP/ key

2) CAL CHECK key

3) ZERO/CFM key

4) READ/ /TIMER key

5) ON/OFF key

6) Liquid Crystal Display (LCD)

7) Cuvette alignment indicator

8) Cuvette holder

KEYPAD DESCRIPTION

• ON/OFF: to turn the meter on and off.

• ZERO/CFM: this is a bi-functional key. Just press to zero the meter prior to measurement, or confirm edited values. In

• READ/ /TIMER: this is a multi-functional key. In

calibration mode

press to confirm factory calibration restore.

measurement mode

, press to make a measurement, or press and hold for three seconds to start a pre-programmed countdown prior to measurement. In

GLP mode

press to view the next screen.

• CAL CHECK: this is a bi-functional key. Just press to perform the validation of the meter, or press and hold for three seconds to enter

• GLP/ : this is a bi-functional key. Just press to enter

calibration mode

GLP mode

. In

calibration mode

press

to edit the date and time.

OPERATING MODES

•

Measurement mode:

•

Calibration mode:

default operation mode, enables both validation and measurement.

may be entered by keeping CAL CHECK pressed for three seconds (the

“CAL” tag appears), it enables calibration of the instrument.

•

GLP mode

may be entered by pressing GLP/ (“GLP” appears), it enables consulting of

user calibration date or restore factory calibration.

DISPLAY ELEMENTS DESCRIPTION

1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or reading measurement

2) Error messages and warnings

3) The battery icon indicates the charge state of the battery

4) The hourglass appears when an internal check is in progress

5) Status messages

6) The chronometer appears when the reaction timer is running

7) The month, day and date icons appear when a date is displayed

8) Four digit main display

9) Measuring units

10) Four digit secondary display

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