Hanna Instruments HI 95747C User Manual

Instruction Manual

HI 95747C

Copper Low Range ISM

www.hannainst.com

This Instrument is in

Compliance with the CE Directives

1

Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary

information for the correct use of the instrument. Please read it carefully before using the meter.
If you need additional technical information, do not hesitate to e-mail us at [e-mail].

This instrument is in compliance with

directives.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION .............................................................................................. 3

GENERAL DESCRIPTION ..................................................................................................... 4

ABBREVIATIONS ................................................................................................................4

SPECIFICATIONS .................................................................................................................5

PRECISION AND ACCURACY ................................................................................................ 5

PRINCIPLE OF OPERATION .................................................................................................6

FUNCTIONAL DESCRIPTION ................................................................................................ 7

GUIDE TO DISPLAY CODES .................................................................................................9

GENERAL TIPS FOR AN ACCURATE MEASUREMENT ........................................................... 11

MEASUREMENT PROCEDURE ...........................................................................................13

VALIDATION PROCEDURE ................................................................................................. 15

CALIBRATION PROCEDURE ...............................................................................................16

BATTERY REPLACEMENT ..................................................................................................18

ACCESSORIES .................................................................................................................18

CE DECLARATION OF CONFORMITY ...................................................................................19

WARRANTY .................................................................................................................19

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

2

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.

Each HI 95747 Ion Selective Meter is supplied complete with:

• Two Sample Cuvets and Caps

• 9V Battery

• Scissors

• Two CAL CHECK

• Tissue for wiping cuvets

• Instruction Manual and Quick Reference Guide

• Instrument Quality Certificate

• Rigid carrying case

TM

Cuvets (HI 95747-11) with Quality Certificate

Note:save all packing material until you are sure that the instrument works correctly.

Any defective item must be returned in its original packing.

3

GENERAL DESCRIPTION

The HI 95747 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has the advanced optical
system based on a special tungsten lamp and a narrow band interference filter that allows most
accurate and repeatable readings. All instruments are factory calibrated and the electronic and
optical design minimizes the need of frequent calibration.

With the powerful CAL CHECKTM validation function you are able to validate good performance
of your instrument at any time. The validation procedure is extremely user friendly. Just use the
exclusive Hanna ready-made, NIST traceable standards to verify the performance of the
instrument and recalibrate if necessary.

All instruments are splash proof and the lamp and filter units are protected from dust or dirt by
a transparent cup. This makes the instruments fulfill field applications. Display codes aid the
user in routine operation. The meter has an auto-shut off feature that will turn off the instrument
after 10 minutes of non use or after 1 hour if left in the

The meter uses an exclusive positive-locking system to ensure that the cuvet is in the same
position every time it is placed into the measurement cell. It is designed to fit a cuvet with a
larger neck making it easier to add both sample and reagents. The cuvet is made from special
optical glass to obtain best results.

The HI 95747 meter measures the free dissolved

0.000 to 1.500 mg/L (ppm) range. The method is an adaptation of the USEPA approved
bicinchoninate method.

The reagent is in
dosed to ensure the maximum repeatability.

powder form and is supplied in packets. The amount of reagent is precisely

calibration mode

Copper Copper

Copper (Cu) content in water samples in the

Copper Copper

.

ABBREVIATIONS

degree Celsius

°C:

degree Fahrenheit

°F:

milligrams per liter. mg/L is equivalent to ppm (part per million)

mg/L:

milliliter

mL:

United States Environmental Protection Agency

USEPA:

4

SPECIFICATIONS

Range 0.000 to 1.500 mg/L
Resolution 0.001 mg/L for

0.01 mg/L for

Precision ±0.015 mg/L @ 0.750 mg/L
Typical EMC Deviation ±0.001 mg/L
Light Source Tungsten lamp with narrow band interference filter @ 560 nm
Light Detector Silicon Photocell
Method Adaptation of the USEPA approved bicinchoninate method. The reaction

between Copper and the bicinchoninate reagent causes a purple tint in
the sample.

Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 1 x 9 volt
Auto-Shut off

Dimensions 180 x 83 x 46 mm (7.1 x 3.3 x 1.8")
Weight 290 g (10 oz.)

REQUIRED REAGENTS
Code Description Quantity/test

HI 95747-0 Copper Low Range Reagent 1 packet

After 10' of non-use in
after 1 hour of non-use in

measurement

calibration

measurement mode

and

validation

calibration mode

;

.

PRECISION AND ACCURACY

Precision is how closely repeated measurements
agree with each other. Precision is usually expressed
as standard deviation (SD).

Accuracy is defined as the nearness of a test result to
the true value.

Although good precision suggests good accuracy,
precise results can be inaccurate. The figure explains
these definitions.

In a laboratory using a standard solution of 0.750
mg/L Copper and a representative lot of reagent, an
operator obtained with a single instrument a standard
deviation of 0.015 mg/L.

5

PRINCIPLE OF OPERATION

Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by
atoms, molecules or crystal lattices.

If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the
Lambert-Beer Law:

Where:

-log I/I

= Absorbance (A)

o

Io= intensity of incident light beam
I = intensity of light beam after absorption

ε

= molar extinction coefficient at wavelength λ

λ

c = molar concentration of the substance
d = optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are known.

Photometric chemical analysis is based on the possibility to develop an absorbing compound
from a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements.

The optical system of Hanna's HI 95 series colorimeters is based on special subminiature
tungsten lamps and narrow-band interference filters to guarantee both high performance and
reliable results.

physical-chemical characteristics of the substance according to the

-log I/Io = ε

λ

c d

or

A = ε

c d

λ

HI 95 series block diagram (optical layout)

6

A microprocessor controlled special tungsten lamp emits radiation which is first optically
conditioned and beamed to the sample contained in the cuvet. The optical path is fixed by the
diameter of the cuvet. Then the light is spectrally filtered to a narrow spectral bandwidth, to
obtain a light beam of intensity Io or I.

The photoelectric cell collects the radiation I that is not absorbed by the sample and converts
it into an electric current, producing a potential in the mV range.

The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.

The measurement process is carried out in two phases: first the meter is zeroed and then the
actual measurement is performed.

The cuvet has a very important role because it is an optical element and thus requires particular
attention. It is important that both the measurement and the calibration (zeroing) cuvets are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvet for both. It is necessary that the surface of the cuvet is clean and not scratched. This to
avoid measurement interference due to unwanted reflection and absorption of light. It is
recommended not to touch the cuvet walls with hands.

Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvet to prevent any contamination.

FUNCTIONAL DESCRIPTION

1) Liquid Crystal Display (LCD)

2) Cuvet Holder

3) Cuvet alignment indicator

4) ON/OFF key

5) ZERO key

6) READ/TIMER key

7) CAL CHECK

7

TM

key

KEYPAD DESCRIPTION

• ON/OFF: to turn the meter on and off.

• ZERO: to zero the meter prior to measurement.

• READ/TIMER: this is a bi-functional key, just press for making a measurement, or hold the
key for 3 seconds to start a pre-programmed countdown prior to measurement.

• CAL CHECK
pressing the CAL CHECKTM key, or hold the key for 3 seconds to enter the

TM

: this bi-functional key allows to perform validation of the instrument just

calibration mode

OPERATING MODES

Measurement mode:

•

•

Calibration mode:

default operation mode, enables both validation and measurement.

may be entered by keeping CAL CHECKTM pressed for three seconds (the

“CAL” icon appears), it enables calibration of the instrument.

DISPLAY ELEMENTS DESCRIPTION

.

1) four digit main display area.

2) CAL: appears when the instrument operates in the

calibration mode.

3) the Month and Day icons appear above the number of month and day when a date is
displayed.

4) the blinking “Battery” icon appears when the battery voltage is getting low.

8

GUIDE TO DISPLAY CODES

This prompt appears for 1 second each time the instrument is
turned on.

This indicates that the meter is in a ready state and zeroing can
be performed.

Sampling in Progress. This
meter is performing a measurement.

This indicates that the meter is in a zeroed state and measurement
can be performed.

Light over range: the cuvet is not inserted correctly and an excess
ambient light is reaching the detector. If the cover is properly
installed, then contact your dealer or the nearest Hanna Customer
Service Center.

The blinking "BAT" indicates that the battery voltage is getting
low and the battery needs to be replaced.

This indicates that the battery is dead and must be replaced.
Once this indication is displayed, the meter will lock up. Change
the battery and restart.

“Configuration”: this indicates that the meter has lost its
configuration. Contact your dealer or the nearest Hanna Customer
Service Center.

CALIBRATION MODE MESSAGES

The date of the last calibration appears on the LCD each time the

calibration mode

first time, “F.CAL” appears.

is entered. If calibration is performed for the

flashing prompt appears each time the

This indicates that the factory calibration is selected.

9

The flashing digits indicate that:

1) the month can be set

2) the day can be set

“Storage”: this prompt appears for 1 second at the end of the
calibration procedure, to indicate that the calibration data has
been stored.

“Error”: the concentration of the calibration solution used is not
correct. Repeat the calibration procedure with the right standard
solution, and verify it is not expired. If the calibration procedure fails
again, contact your dealer or the nearest Hanna Customer Service
Center.

ERROR MESSAGES

a) on zero reading

This indicates that the zeroing procedure failed due to a low signal­to-noise ratio. In this case press ZERO again.

“No Light”: the instrument cannot adjust the light level. Please check
that the sample does not contain any debris.

“Light Low”: there is not enough light to perform a measurement.
Please check the preparation of the zero cuvet.

“Light High”: there is too much light to perform a measurement.
Please check the preparation of the zero cuvet.

b) on sample reading

There is too much light for the sample measurement. Please check
if the right sample cuvet is inserted.

“Inverted”: the sample and the zero cuvet are inverted.

“Zero”: a zero reading was not taken. Follow the instruction in
the measurement procedure for zeroing the meter.

10

Under range. A blinking "0.000" indicates that the sample
absorbs less light than the zero reference. Check the procedure
and make sure you use the same cuvet for reference (zero) and
measurement.

A flashing value of the maximum concentration indicates an
over range condition. The concentration of the sample is beyond
the programmed range: dilute the sample and re-run the test.

A flashing value lower than the maximum concentration indicates
a low signal-to-noise ratio condition. In this case accuracy of the
result is not guaranteed. Repeat the measurement procedure.

GENERAL TIPS FOR AN ACCURATE MEASUREMENT

The instructions listed below should be carefully followed during testing to ensure best accuracy.

• Color or suspended matter in large amounts
may cause interference, therefore these should
be removed by treatment with active carbon and
by prior filtration.

• For a correct filling of the cuvet: the liquid in
the cuvet forms a convexity on the top; the
bottom of this convexity must be at the same
level of the 10 mL mark.

• Proper use of the powder reagent packet:
(a) use scissors to open the powder packet;

(b) push the edges of the packet to form a spout;
(c) pour out the content of the packet.

(a) (b) (c)

11

• It is important that the sample does not contain
any debris. This would corrupt the reading.

• In order to avoid reagent leaking and to obtain
more accurate measurements, it is recommended
to close the cuvet first with the supplied HDPE
plastic stopper and then with the black cap.

• Each time the cuvet is used, the cap must be
tightened to the same degree.

• Whenever the cuvet is placed into the measurement
cell, it must be dry outside, and
of fingerprints, oil or dirt. Wipe it thoroughly with
HI 731318 (tissue for wiping cuvets, see chapter
ACCESSORIES) or a lint-free cloth prior to insertion.

• Do not let the reacted sample stand too long after
reagent is added, or accuracy will be lost.

• It is possible to take multiple readings in a row,
but it is recommended to take a new zero reading
for each sample and to use the same cuvet for
zeroing and measurement.

completely free

• After the reading it is important to discard
immediately the sample, otherwise the glass
might become permanently stained.

• All the reaction times reported in this manual are
referred to 20°C (68°F). As a general rule of
thumb, they should be doubled at 10°C (50°F)
and halved at 30°C (86°F).

• In order to maximize accuracy, prior to a
measurement follow the validation procedure
to be sure that the instrument is properly
calibrated. If necessary, calibrate the instrument.

12

MEASUREMENT PROCEDURE

Turn the meter on by pressing ON/OFF and follow instruction manual to perform either the
MEASUREMENT, VALIDATION or CALIBRATION PROCEDURE.

MEASUREMENT PROCEDURE

• Turn the meter on by pressing ON/OFF.

• When the LCD displays “747”, it is ready.

• Fill the cuvet with 10 mL of unreacted sample,
up to the mark, and replace the cap.

• Place the cuvet into the holder and ensure that
the notch on the cap is positioned securely into
the groove.

• Press ZERO and “SIP” will blink on the display.

• After a few seconds the display will show
“ -0.0- ”. The meter is now zeroed and ready
for measurement.

• Remove the cuvet.

• Add the content of one packet of HI 95747-0
Copper Low Range reagent. Replace the cap
and shake gently for 15 seconds.

• Replace the cuvet into the holder and ensure
that the notch on the cap is positioned securely
into the groove.

13

• Hold READ/TIMER for three seconds. The
display will show the countdown prior to
measurement.

Alternatively, wait for 45 seconds and just
press READ/TIMER.

In both cases “SIP” will blink during
measurement.

• The instrument directly displays concentration
in mg/L of copper on the Liquid Crystal Display.

INTERFERENCES

• Cyanide

• Silver

• For strongly buffered alkaline or acidic samples, pH should be adjusted between 6 and 8

before addition of reagent.

• To avoid interferences due to fingerprints, oil or dirt it is very important that the cuvet is

wiped clean prior to insertion in the cuvet holder.
Replacement of scratched cuvets is strongly recommended.

14

VALIDATION PROCEDURE

Use the validation procedure to ensure that the instrument is properly calibrated.
Warning: do not validate or calibrate the instrument with standard solutions other than the Hanna
CAL CHECKTM Standards, otherwise erroneous results will be obtained. For accurate Validation and
Calibration results, please perform test at room temperature: 18 to 25°C (64.5 to 77.0°F).

• Turn the meter on by pressing ON/OFF.
When the LCD displays “747”, it is ready.

• Place the CAL CHECK

TM

Standard Cuvet A into

the holder and ensure that the notch on the
cap is positioned securely into the groove.

• Press ZERO and “SIP” will blink on the display.

• After a few seconds the display will show
“-0.0-”. The meter is now zeroed and ready for
validation.

• Remove the cuvet and place the CAL CHECK

TM

Standard HI 95747-11 Cuvet B into the holder.
Ensure that the notch on the cap is positioned
securely into the groove.

• Press CAL CHECK

TM

and “SIP” will blink during

measurement.

• Wait for a few seconds and the display will show the validation standard value.
The reading should be within specifications as reported on the CAL CHECK

TM

Certificate. If the value found is out of specifications, please check that the cuvets are free of
fingerprints, oil or dirt and repeat validation. If results are still found out of specifications
then recalibrate the instrument.

Standard

15

CALIBRATION PROCEDURE

Note: it is possible to interrupt calibration procedure at any time by pressing ON/OFF.
Warning: do not validate or calibrate the instrument with standard solutions other than the Hanna

CAL CHECKTM Standards, otherwise erroneous results will be obtained. For accurate Validation and
Calibration results, please perform test at room temperature: 18 to 25°C (64.5 to 77.0°F).

CALIBRATION PROCEDURE

• Turn the meter on by pressing ON/OFF.
When the LCD displays “747”, it is ready.

• Enter the
CHECK

calibration mode

TM

for three seconds.

by holding CAL

• The date of the last calibration appears (e.g.:
month “01”, day “08”). “F.CAL” means that
the factory calibration is selected.

Note: at this point it is possible to reset the
instrument to restore FACTORY CALIBRATION,
see instructions page 18.

• Place the CAL CHECKTM Standard Cuvet A into
the holder and ensure that the notch on the
cap is positioned securely into the groove.

• Press ZERO and “SIP” will blink on the display.

• After a few seconds the display will show
“-0.0-”. The meter is now zeroed and ready for
calibration.

• Remove the cuvet.

TM

• Place the CAL CHECK

Standard HI 95747-11

Cuvet B into the holder and ensure that the
notch on the cap is positioned securely into
the groove.

16

• Press READ/TIMER and “SIP” will blink during
measurement.

• The instrument will show for three seconds the

TM

CAL CHECK

standard value.

Then the date of last calibration (e.g.:
“01.08”) appears on the display, or “01.01”
if the factory calibration was selected. In both
cases the number of the month is blinking,
ready for date input.

Note: if the display shows “ERR” the calibration
procedure failed. Verify that the CAL CHECK
Standards HI 95747-11 Cuvets, A and B, are
free from fingerprints or dirt and that they are
inserted correctly.

DATE INPUT

• Keep READ/TIMER pressed to scroll to the
desired month number (01-12).

• When the correct month has been set, press
ZERO to confirm. Now the display will show
the day number blinking. Keep READ/TIMER
pressed to scroll to the desired day number
(01-31).

Note: it is possible to change from

month

and vice versa by pressing ZERO.

day

TM

to

• When both the day and month have been
selected, hold CAL CHECKTM for three seconds
to store date and calibration values. The
instrument will show for one second “Stor”, to
confirm that the new calibration data has been
accepted.

• The instrument will return automatically to the
measurement mode by displaying "747" on
the LCD.

17

FACTORY CALIBRATION RESET

It is possible to restore factory calibration:

• Enter the
CHECKTM for three seconds.

• Hold ZERO for 10 seconds. The display will
show for 2 seconds “F.CAL” and "747" appears.
The factory calibration is automatically restored
and the instrument is ready for measurement.

calibration mode

by holding CAL

BATTERY REPLACEMENT

Battery replacement must only take place in a
non-hazardous area.

Simply slide off the battery cover on the back of
the meter. Detach the battery from the terminals
and attach a fresh 9V battery while paying attention
to the correct polarity. Insert the battery and
replace the cover.

ACCESSORIES

REAGENT SETS
HI 95747-01 Reagents for 100 tests
HI 95747-03 Reagents for 300 tests
OTHER ACCESSORIES

HI 95747-11 CAL CHECKTM Standard Cuvets

HI 710009 Blue rubber boot
HI 710010 Orange rubber boot
HI 721310 9V battery (10 pcs)
HI 731318 Tissue for wiping cuvets (4 pcs)
HI 731331 Glass cuvets (4 pcs)
HI 731335 Caps for cuvets (4 pcs)
HI 93703-50 Cuvets cleaning solution (230 mL)

(1 set)

18

CE DECLARATION OF CONFORMITY

Recommendations for Users

Before using these products, make sure that they are
entirely suitable for your specific application and for the
environment in which they are used.

Operation of these instruments may cause unacceptable
interferences to other electronic equipments, this requiring
the operator to take all necessary steps to correct
interferences.

Any variation introduced by the user to the supplied
equipment may degrade the instruments' EMC
performance.

To avoid damages or burns, do not put the instrument in
microwave oven. For yours and the instrument safety do
not use or store the instrument in hazardous environments.

WARRANTY

HI 95747 is warranted for two years against defects in workmanship and materials when used
for its intended purpose and maintained according to the instructions.

This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of

purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.

If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods
Authorization Number from the Customer Service Department and then send it with shipment
costs prepaid. When shipping any instrument, make sure it is properly packaged for complete
protection.

To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.

Hanna Instruments reserves the right to modify the design, construction and appearance of its
products without advance notice.

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SALES AND TECHNICAL SERVICE CONTACTS

Australia:

Tel. [phone] • Fax [phone]

China:

Tel. [phone] • Fax [phone]

Egypt:

Tel. & Fax [phone]

Germany:

Tel. [phone] • Fax [phone]

Greece:

Tel. [phone] • Fax [phone]

Indonesia:

Tel. [phone] • Fax [phone]

Japan:

Tel. [phone] • Fax [phone]

Korea:

Tel. [phone] • Fax [phone]

Malaysia:

Tel. [phone] • Fax [phone]

Singapore:

Tel. [phone] • Fax [phone]

South Africa:

Tel. [phone] • Fax [phone]

Taiwan:

Tel. [phone] • Fax [phone]

Thailand:

Tel. [phone] • Fax [phone]

MAN95747CR1 11/03

United Kingdom:

Tel. [phone] • Fax [phone]

USA:

Tel. [phone] • Fax [phone]

For e-mail contacts and complete list of Sales and Technical offices,
please see www.hannainst.com

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