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Instruction Manual
HI 95734C
Free & Total Chlorine
High Range ISM
www.hannainst.com
24
MAN95734R3 07/06
www.hannainst.com
This Instrument is in
Compliance with the CE Directives
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Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.
This instrument is in compliance with
directives.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION ................................................................................................ 3
GENERAL DESCRIPTION......................................................................................................... 4
ABBREVIATIONS...................................................................................................................4
SPECIFICATIONS ..................................................................................................................5
PRECISION AND ACCURACY................................................................................................... 5
PRINCIPLE OF OPERATION .................................................................................................... 6
FUNCTIONAL DESCRIPTION ................................................................................................... 8
GUIDE TO DISPLAY CODES .................................................................................................... 9
GENERAL TIPS FOR AN ACCURATE MEASUREMENT .................................................................12
OPERATIONAL GUIDE ...........................................................................................................14
MEASUREMENT PROCEDURE ................................................................................................15
VALIDATION PROCEDURE .....................................................................................................17
CALIBRATION PROCEDURE ...................................................................................................18
ACCESSORIES .....................................................................................................................20
BATTERY REPLACEMENT ....................................................................................................... 21
DECLARATION OF CONFORMITY ..................................................................................... 21
WARRANTY .........................................................................................................................22
HANNA LITERATURE.............................................................................................................22
USER NOTES.......................................................................................................................23
USER NOTES
Date Validation Value
Parameter Code Passed
(mg/L)
yes not
Calibration
yes not
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
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HANNA instruments® reserves the right to modify the design, construction and appearance of its
products without advance notice.
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WARRANTY
PRELIMINARY EXAMINATION
HI 95734 is warranted for two years against defects in workmanship and materials when used for
its intended purpose and maintained according to the instructions.
This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.
If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization
Number from the Customer Service Department and then send it with shipment costs prepaid.
When shipping any instrument, make sure it is properly packaged for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.
HANNA LITERATURE
Hanna publishes a wide range of catalogs and handbooks for an equally wide range of applications.
The reference literature currently covers areas such as:
Please examine this product carefully. Make sure that the instrument is not damaged. If any damage
occured during shipment, please notify your Dealer.
Each HI 95734 Ion Selective Meter is supplied complete with:
• Two Sample Cuvets and Caps
• 9V Battery
• Scissors
• Two CAL CHECK™ Cuvets (HI 95734-11) with Quality Certificate
• Tissue for wiping cuvets
• Instruction Manual and Quick Reference Guide
• Instrument Quality Certificate
• Rigid carrying case
• Water Treatment
• Process
• Swimming Pools
• Agriculture
• Food
• Laboratory
and many others. New reference material is constantly being added to the library.
For these and other catalogs, handbooks and leaflets contact your dealer or the Hanna Customer
Service Center nearest to you. To find the Hanna Office in your vicinity, check our home page at
www.hannainst.com.
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Note:save all packing material until you are sure that the instrument works correctly.
Any defective item must be returned in its original packing.
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GENERAL DESCRIPTION
9V
SLIDE
OFF
The HI 95734 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has the advanced optical system
based on a special tungsten lamp and a narrow band interference filter that allows most accurate
and repeatable readings. All instruments are factory calibrated and the electronic and optical
design minimizes the need of frequent calibration.
With the powerful CAL CHECK™ validation function you are able to validate good performance of
your instrument at any time. The validation procedure is extremely user friendly. Just use the
exclusive Hanna ready-made, NIST traceable standards to verify the performance of the instrument
and recalibrate if necessary.
All instruments are splash waterproof and the lamp and filter units are protected from dust or dirt by
a transparent cup. This makes the instruments fulfill field applications. Display codes aid the user in
routine operation. The meter has an auto-shut off feature that will turn off the instrument after 10
minutes of non use or after 1 hour if left in the calibration mode.
The meter uses an exclusive positive-locking system to ensure that the cuvet is in the same position
every time it is placed into the measurement cell. It is designed to fit a cuvet with a larger neck
making it easier to add both sample and reagents. The cuvet is made from special optical glass to
obtain best results.
The HI 95734 meter measures the free and total chlorine (Cl
0.00 to 10.00 mg/L (ppm) range. The method is an adaption of the USEPA METHOD 330.5 for
wastewater, and Standard Method 4500-Cl G for drinking water.
The reagents are in
powder and liquid form and are supplied in packets and bottles. The amount of
reagent is precisely dosed to ensure the maximum repeatability.
) content in water samples in the
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BATTERY REPLACEMENT
Battery replacement must only take place in a nonhazardous area.
Simply slide off the battery cover on the back of the
meter. Detach the battery from the terminals and attach
a fresh 9V battery while paying attention to the correct
polarity. Insert the battery and replace the cover.
DECLARATION OF CONFORMITY
ABBREVIATIONS
°C:
degree Celsius
EPA:
US Environmental Protection Agency
°F:
degree Fahrenheit
HR:
High Range
mg/L:
milligrams per liter. mg/L is equivalent to ppm (part per million)
mL:
milliliter
Recommendations for Users
Before using these products, make sure that they are entirely suitable for your specific application and for the
environment in which they are used.
Operation of these instruments may cause unacceptable interferences to other electronic equipments, this requiring the
operator to take all necessary steps to correct interferences.
Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC performance.
To avoid damages or burns, do not put the instrument in microwave ovens. For yours and the instrument safety do not
use or store the instrument in hazardous environments.
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FACTORY CALIBRATION RESET
It is possible to restore factory calibration:
• Enter the calibration mode by holding CAL CHECK
for three seconds.
• Hold ZERO/SET for 10 seconds. The display will
show for 2 seconds “F.CAL” and the parameter
code ("734f" or “734t”) appears. The factory
calibration is automatically restored and the
instrument is ready for measurement.
ACCESSORIES
REAGENT SETS
HI 93734-01 Reagents for 100 Free or Total Chlorine HR tests
HI 93734-03 Reagents for 300 Free or Total Chlorine HR tests
OTHER ACCESSORIES
HI 95734-11 CAL CHECK™ Standard Cuvets
HI 710009 Blue rubber boot
HI 710010 Orange rubber boot
HI 721310 9V battery (10 pcs)
HI 731318 Tissue for wiping cuvets (4 pcs)
HI 731331 Glass cuvets (4 pcs)
HI 731335 Caps for cuvets (4 pcs)
HI 740157 Plastic refilling pipette (20 pcs)
HI 740226 5 mL graduated syringe
HI 93703-50 Cuvets cleaning solution (230 mL)
(1 set)
SPECIFICATIONS
Range Free Cl
Resolution 0.01 mg/L from 0.00 to 3.50 mg/L; 0.10 mg/L above 3.50 mg/L
Precision ±0.06 mg/L @ 3.00 mg/L
Light Source Tungsten lamp with narrow band interference filter @ 525 nm
Light Detector Silicon Photocell
Method Adaptation of the USEPA method 330.5 and Standard Method
or
Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type 1 x 9 volt
Auto-Shut off
Dimensions 180 x 83 x 46 mm (7.1 x 3.3 x 1.8")
Weight 290 g (10 oz.).
REQUIRED REAGENTS
Code Description Quantity/test
HI 93701-0 Free Chlorine
HI 93734B-0 Free & Total Chlorine HR
HI 93734C-0 Total Chlorine HR Reagent 3 drops
0.00 to 10.00 mg/L
2
Total Cl
0.00 to 10.00 mg/L
2
4500-Cl G. The reaction between chlorine and the DPD reagent
causes a pink tint in the sample.
After 10' of non-use in measurement mode;
after 1 hour of non-use in calibration mode.
Reagent 1 packet
Reagent 5 mL
PRECISION AND ACCURACY
Precision is how closely repeated
measurements agree with each other.
Precision is usually expressed as standard
deviation (SD).
nearness of a test result to the true value.
Although good precision suggests good
accuracy, precise results can be inaccurate. The
figure explains these definitions.
In a laboratory using a standard solution of
3.00 mg/L chlorine and a representative lot of
reagent, an operator obtained with a single
instrument a standard deviation of 0.06 mg/L
Accuracy is defined as the
.
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PRINCIPLE OF OPERATION
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through
the matter and on the
Beer Law:
Where:
-log I/I
I
I = intensity of light beam after absorption
ε
λ
c = molar concentration of the substance
d = optical path through the substance
physical-chemical characteristics of the substance according to the Lambert-
-log I/I
= ε
o
λ
c d
or
c d
A = ε
λ
= Absorbance (A)
o
= intensity of incident light beam
o
= molar extinction coefficient at wavelength λ
• Press READ/TIMER and “SIP” will blink during
measurement.
• The instrument will show for three seconds the
CAL CHECK™ standard value.
Then the date of last calibration (e.g.: “01.08”)
appears on the display, or “01.01” if the factory
calibration was selected. In both cases the
number of the month is blinking, ready for date
input.
Note: if the display shows “ERR” the calibration
procedure failed. Verify that both CAL CHECK™
Standards HI 95734-11 Cuvets, A and B, are
free from fingerprints or dirt and that they are
inserted correctly.
DATE INPUT
• Keep READ/TIMER pressed to scroll to the desired
month number (01-12).
or
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other
factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound from a
specific chemical reaction between sample and reagents. Given that the absorption of a compound
strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should be
selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna's HI 95 series colorimeters is based on special subminiature tungsten
lamps and narrow-band interference filters to guarantee both high performance and reliable results.
Block diagram (optical layout)
6
• When the correct month has been set, press
ZERO to confirm. Now the display will show the
day number blinking. Keep READ/TIMER pressed
to scroll to the desired day number (01-31).
Note: it is possible to change from day to
month and vice versa by pressing ZERO.
• When both the day and month have been
selected, hold CAL CHECK
for three seconds to
store date and calibration values. The instrument
will show for one second “Stor”, to confirm that
the new calibration data has been accepted.
• The instrument will return automatically to the
measurement mode by displaying the parameter
code (“734f” or “734t”) on the LCD.
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or
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CALIBRATION PROCEDURE
Note: it is possible to interrupt calibration procedure at any time by pressing ON/OFF.
Warning: do not validate or calibrate the instrument with standard solutions other than the Hanna
CAL CHECK™ Standards, otherwise erroneous results will be obtained.
For accurate Validation and Calibration results, please perform test at room temperature: 18 to 25°C
(64.5 to 77.0°F).
CALIBRATION PROCEDURE
• Select the parameter code for free chlorine “734f”
or total chlorine “734t” by holding ZERO/SET
(see page 14).
• Enter the calibration mode by holding CAL CHECK
for three seconds.
• The date of the last calibration appears (e.g.:
month “01”, day “08”). “F.CAL” means that
the factory calibration is selected.
Note: at this point it is possible to reset the
instrument to restore FACTORY CALIBRATION,
see instructions page 20.
• Place the CAL CHECK™ Standard Cuvet A into
the holder and ensure that the notch on the cap is
positioned securely into the groove.
or
A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned
and beamed to the sample contained in the cuvet. The optical path is fixed by the diameter of the
cuvet. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of
intensity I
or I.
o
The photoelectric cell collects the radiation I that is not absorbed by the sample and converts it
into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual
measurement is performed.
The cuvet has a very important role because it is an optical element and thus requires particular
attention. It is important that both the measurement and the calibration (zeroing) cuvets are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvet for both. It is necessary that the surface of the cuvet is clean and not scratched. This to avoid
measurement interference due to unwanted reflection and absorption of light. It is recommended
not to touch the cuvet walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvet to prevent any contamination.
• Press ZERO and “SIP” will blink on the display.
• After a few seconds the display will show “-0.0-”.
The meter is now zeroed and ready for calibration.
• Remove the cuvet.
• Place the CAL CHECK™ Standard HI 95734-11
Cuvet B into the holder and ensure that the
notch on the cap is positioned securely into the
groove.
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FUNCTIONAL DESCRIPTION
1) Liquid Crystal Display (LCD)
2) Cuvet Holder
3) Cuvet alignment indicator
4) ON/OFF key
5) ZERO/SET key
6) READ/TIMER key
7) CAL CHECK key
KEYPAD DESCRIPTION
• ON/OFF: to turn the meter on and off.
• ZERO/SET: this is a bi-functional key: press to zero the meter prior to measurement, or hold the
key for 3 seconds to select the desired parameter (free or total chlorine).
• READ/TIMER: this is a bi-functional key, just press for making a measurement, or hold the key
for 3 seconds to start a pre-programmed countdown prior to measurement.
• CAL CHECK: this bi-functional key allows to perform validation of the instrument just pressing
the CAL CHECK key, or hold the key for 3 seconds to enter the calibration mode.
VALIDATION PROCEDURE
Use the validation procedure to ensure that the instrument is properly calibrated.
Warning: do not validate or calibrate the instrument with standard solutions other than the Hanna
CAL CHECK™
For accurate Validation and Calibration results, please perform test at room temperature: 18 to 25°C
(64.5 to 77.0°F).
Temperature correction for Validation: corrected value = read value × [1 + 0.002 × (20 − T(°C))]
• Select the parameter code for free chlorine “734f”
• Place the CAL CHECK™ Standard Cuvet A into
• Press ZERO and “SIP” will blink on the display.
• After a few seconds the display will show “-0.0-”.
• Remove the cuvet.
Standards, otherwise erroneous results will be obtained.
corrected value = read value × [1 + 0.001 × (68 − T(°F))]
or total chlorine “734t” by holding ZERO/SET
(see page 14).
the holder and ensure that the notch on the cap is
positioned securely into the groove.
The meter is now zeroed and ready for validation.
OPERATING MODES
• Measurement mode: default operation mode, enables both validation and measurement.
• Calibration mode: may be entered by keeping CAL CHECK pressed
for three seconds (the “CAL”
icon appears), it enables calibration of the instrument.
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• Place the CAL CHECK™ Standard HI 95734-11
Cuvet B into the holder and ensure that the notch
on the cap is positioned securely into the groove.
• Press CAL CHECK and “SIP” will blink during
measurement.
• Wait for a few seconds and the display will show the validation standard value.
The reading should be within specifications as reported on the CAL CHECK™ Standard Certificate.
If the value is found out of specifications, please check that the cuvets are free of fingerprints, oil or
dirt and repeat validation. If results are still found out of specifications then recalibrate the
instrument.
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• Only in the case of total chlorine measurement,
add 3 drops of HI 93734C-0 reagent to the
cuvet.
• For both free and total chlorine, add the content
of one packet of HI 93701-0 Free Chlorine
reagent. Replace the cap and shake gently for
20 seconds (or 2 minutes in case of seawater
analysis).
• Replace the cuvet into the holder and ensure
that the notch on the cap is positioned securely
into the groove.
for Total
Chlorine
only
DISPLAY ELEMENTS DESCRIPTION
1) four digit main display area.
2) CAL: appears when the instrument operates in the calibration mode.
3) the Month and Day icons appear above the number of month and day when a date is displayed.
4) the blinking “Battery” icon appears when the battery voltage is getting low.
• Hold READ/TIMER
for three seconds. The display
will show the countdown prior to measurement.
Alternatively, wait for:
Free Chlorine: Total Chlorine:
1 minute 2 minutes and 30 seconds
or
or
Then press READ/TIMER.
In both cases “SIP” will blink during
measurement.
• The instrument directly displays concentration
in mg/L of free or total chlorine on the Display.
INTERFERENCES
Bromine (positive error) Oxidized Manganese and Chromium (positive error)
Chlorine dioxide (positive error) Ozone (positive error)
Iodine (positive error)
Alkalinity above 250 mg/L CaCO
or acidity above 150 mg/L CaCO3 will not reliably develop the
3
full amount of color or it may rapidly fade. To resolve this, neutralize the sample with diluted HCl
or NaOH.
In case of water with hardness greater than 500 mg/L CaCO
, shake the sample for approximately
3
2 minutes after adding the powder reagent.
GUIDE TO DISPLAY CODES
This prompt appears for 1 second each time the instrument is
turned on.
The parameter code, “734f” for free chlorine or “734t” for total
chlorine, indicates that the meter is in a ready state and zeroing
can be performed.
Sampling in Progress. This
meter is performing a measurement.
This indicates that the meter is in a zeroed state and measurement
can be performed.
Light over range: the cuvet is not inserted correctly and an excess
ambient light is reaching the detector. If the cover is properly
installed, then contact your dealer or the nearest Hanna Customer
Service Center.
flashing prompt appears each time the
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The blinking "BAT" indicates that the battery voltage is getting low
and the battery needs to be replaced.
MEASUREMENT PROCEDURE
This indicates that the battery is dead and must be replaced. Once
this indication is displayed, the meter will lock up. Change the
battery and restart.
“Configuration”: this indicates that the meter has lost its
configuration. Contact your dealer or the nearest Hanna Customer
Service Center.
CALIBRATION MODE MESSAGES
The date of the last calibration appears on the LCD each time the
calibration mode is entered. If calibration is performed for the first
time, “F.CAL” appears.
This indicates that the factory calibration is selected.
The flashing digits indicate that:
1) the month can be set
2) the day can be set
• Select the parameter code for free chlorine
“734f” or total chlorine “734t” by holding
ZERO/SET (see page 14).
• Add to the cuvet 5 mL of HI 93734B-0 reagent
by means of the 5 mL syringe.
Note: in order to measure exactly 5 mL of
reagent with the syringe, follow the instructions
on page 12.
• Fill the cuvet up to the 10 mL mark with 5 mL
of unreacted sample, using the 3 mL plastic
pipette.
Note: rinse the 3 mL plastic pipette several
times with sample before adding it to the cuvet.
• Replace the cap and shake gently for some
seconds.
• Place the cuvet into the holder and ensure that
the notch on the cap is positioned securely into
the groove.
“Storage”: this prompt appears for 1 second at the end of the
calibration procedure, to indicate that the calibration data has
been stored.
“Error”: the concentration of the calibration solution used is not
correct. Repeat the calibration procedure with the right standard
solution, and verify it is not expired. If the calibration procedure
fails again, contact your dealer or the nearest Hanna Customer
Service Center.
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• Press ZERO/SET and “SIP” will blink on the
display.
• After a few seconds the display will show
“-0.0-”. The meter is now zeroed and ready for
measurement.
• Remove the cuvet.
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OPERATIONAL GUIDE
ERROR MESSAGES
a) on zero reading
• Turn the meter on by pressing ON/OFF.
• When the LCD displays “734f” or “734t” it is
ready. The display code that appears is the one
of the last selected parameter.
Code Parameter
734f Free Chlorine
734t Total Chlorine
• keep ZERO/SET pressed to change parameter.
The parameter code will change every three
seconds, until the key is released. Select the
program for free chlorine “734f” or total chlorine
“734t”.
• After the desired parameter code appears on
the display, perform either MEASUREMENT,
VALIDATION, or CALIBRATION PROCEDURE
following the instructions reported in the related
chapter.
This indicates that the zeroing procedure failed due to a low signal-tonoise ratio. In this case press ZERO again.
“No Light”: the instrument cannot adjust the light level. Please check
or
that the sample does not contain any debris.
“Light Low”: there is not enough light to perform a measurement.
Please check the preparation of the zero cuvet.
“Light High”: there is too much light to perform a measurement.
Please check the preparation of the zero cuvet.
b) on sample reading
There is too much light for the sample measurement. Please check if
the right sample cuvet is inserted.
“Inverted”: the sample and the zero cuvet are inverted.
“Zero”: a zero reading was not taken. Follow the instruction in
the measurement procedure for zeroing the meter.
Under range. A blinking "0.00" indicates that the sample absorbs
less light than the zero reference. Check the procedure and make
sure you use the same cuvet for reference (zero) and measurement.
Important Note: Free and Total Chlorine have to be measured separately with fresh unreacted
•
samples if both values are requested.
14
A flashing value of the maximum concentration indicates an over
range condition. The concentration of the sample is beyond the
programmed range: dilute the sample and re-run the test.
A flashing value lower than the maximum concentration indicates
a low signal-to-noise ratio condition. In this case accuracy of the
result is not guaranteed. Repeat the measurement procedure.
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GENERAL TIPS FOR AN ACCURATE MEASUREMENT
The instructions listed below should be carefully followed during testing to ensure best accuracy.
• For a correct filling of the cuvet: the liquid in the
cuvet forms a convexity on the top; the bottom of
this convexity must be at the same level of the 10
mL mark.
• It is important that the sample does not contain
any debris. This would corrupt the reading.
• Proper use of the dropper:
(a) to get good reproducible results, tap the dropper on the table for several times and wipe the
outside of the dropper tip with a cloth.
(b) always keep the dropper bottle in a vertical position while dosing the reagent.
• Each time the cuvet is used, the cap must be
tightened to the same degree.
• Whenever the cuvet is placed into the measurement
cell, it must be dry outside, and completely free of
fingerprints, oil or dirt. Wipe it thoroughly with
HI 731318 (tissue for wiping cuvets, see chapter
ACCESSORIES) or a lint-free cloth prior to insertion.
• In order to measure exactly 5 mL of reagent with the 5 mL syringe:
(a) push the plunger completely into the syringe and insert the tip into the reagent bottle.
(b) pull the plunger up until the lower edge of the seal is exactly on the 5 mL mark.
(c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are
hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in
vertical position above the cuvet, push the plunger completely down into the syringe. Now
the exact amount of 5 mL has been added to the cuvet.
(a) (b)
• Proper use of the powder reagent packet:
(a) use scissors to open the powder packet;
(b) push the edges of the packet to form a spout;
(c) pour out the content of the packet.
(a)
(b)
(c)
• Shaking the cuvet can generate bubbles in the sample, causing higher readings. To obtain accurate
measurements, remove such bubbles by swirling or by gently tapping the vial.
• Do not let the reacted sample stand too long after reagent is added, or accuracy will be lost.
• It is possible to take multiple readings in a row, but it is recommended to take a new zero
reading for each sample and to use the same cuvet for zeroing and measurement.
• After the reading it is important to discard immediately the sample, otherwise the glass might
become permanently stained.
(a) (b) (c)
12
• All the reaction times reported in this manual are referred to 20°C (68°F). As a general rule of
thumb, they should be doubled at 10°C (50°F) and halved at 30°C (86°F).
• In order to maximize accuracy, prior to a measurement follow the validation procedure to be
sure that the instrument is properly calibrated. If necessary, calibrate the instrument.
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