Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.
Before using these products, make sure that they are
entirely suitable for your specific application and for
the environment in which they are used.
Operation of these instruments may cause
unacceptable interferences to other electronic
equipments, this requiring the operator to take all
necessary steps to correct interferences.
Any variation introduced by the user to the supplied
equipment may degrade the instruments' EMC
performance.
To avoid damages or burns, do not put the instrument
in microwave oven. For yours and the instrument safety
do not use or store the instrument in hazardous
environments.
WARRANTY
HI 95729 is warranted for two years against defects in workmanship and materials when used
for its intended purpose and maintained according to the instructions.
This warranty is limited to repair or replacement free of charge.
Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.
If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization
Number from the Customer Service Department and then send it with shipment costs prepaid.
When shipping any instrument, make sure it is properly packaged for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
2
Hanna Instruments reserves the right to modify the design, construction and appearance of its
products without advance notice.
19
Page 3
9V
SLIDE
OFF
FACTORY CALIBRATION RESET
It is possible to restore factory calibration:
• Enter the calibration mode by holding CAL CHECK
for three seconds.
• Hold ZERO for 10 seconds. The display will
show for 2 seconds “F.CAL” and "729" appears.
The factory calibration is automatically restored
and the instrument is ready for measurement.
BATTERY REPLACEMENT
Battery replacement must only take place in a
non-hazardous area.
Simply slide off the battery cover on the back of
the meter. Detach the battery from the terminals
and attach a fresh 9V battery while paying attention
to the correct polarity. Insert the battery and replace
the cover.
ACCESSORIES
PRELIMINARY EXAMINATION
Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.
Each HI 95729 Ion Selective Meter is supplied complete with:
• Two Sample Cuvets and Caps
• 9V Battery
• Two CAL CHECKTM Cuvets (HI 95729-11) with Quality Certificate
• Tissue for wiping cuvets
• One 2000 µL automatic pipette with Instruction Sheet
• One plastic tip for 2000 µL automatic pipette
• Instruction Manual and Quick Reference Guide
• Instrument Quality Certificate
• Rigid carrying case
REAGENT SETS
HI 93729-01Reagents for 100 tests
HI 93729-03Reagents for 300 tests
OTHER ACCESSORIES
HI 93703-53Chlorine Remover
HI 95729-11CAL CHECKTM Standard Cuvets
(1 set)
HI 710009Blue rubber boot
HI 710010Orange rubber boot
HI 7213109V battery (10 pcs)
HI 731318Tissue for wiping cuvets (4 pcs)
HI 731331Glass cuvets (4 pcs)
HI 731335Caps for cuvets (4 pcs)
HI 7313422000 µL automatic pipette
HI 731352tips for 2000 µL automatic pipette (4 pcs)
HI 93703-50Cuvets cleaning solution (230 mL)
18
Note:save all packing material until you are sure that the instrument works correctly.
Any defective item must be returned in its original packing.
3
Page 4
GENERAL DESCRIPTION
The HI 95729 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s
years of experience as a manufacturer of analytical instruments. It has the advanced optical
system based on a special tungsten lamp and a narrow band interference filter that allows most
accurate and repeatable readings. All instruments are factory calibrated and the electronic and
optical design minimizes the need of frequent calibration.
With the powerful CAL CHECKTM validation function you are able to validate good performance
of your instrument at any time. The validation procedure is extremely user friendly. Just use the
exclusive Hanna ready-made, NIST traceable standards to verify the performance of the
instrument and recalibrate if necessary.
All instruments are splash proof and the lamp and filter units are protected from dust or dirt by
a transparent cup. This makes the instruments fulfill field applications. Display codes aid the
user in routine operation. The meter has an auto-shut off feature that will turn off the instrument
after 10 minutes of non use or after 1 hour if left in the calibration mode.
The meter uses an exclusive positive-locking system to ensure that the cuvet is in the same
position every time it is placed into the measurement cell. It is designed to fit a cuvet with a
larger neck making it easier to add both sample and reagents. The cuvet is made from special
optical glass to obtain best results.
The HI 95729 meter measures the
in drinking, surface and waste waters. The method is an adaptation of the EPA method 340.1
and Standard Methods for the Examination of Water and Wastewater, 20th edition, SPADNS
method.
The reagent is in
by use of the supplied automatic pipette to ensure the maximum repeatability.
liquid form and is supplied in bottles. The amount of reagent is precisely dosed
FluorideFluoride
Fluoride content in the 0.00 to 2.00 mg/L (ppm) range,
FluorideFluoride
• Press READ/TIMER and “SIP” will blink during
measurement.
• The instrument will show for three seconds the
CAL CHECK standard value.
Then the date of last calibration (e.g.:
“01.08”) appears on the display, or “01.01”
if the factory calibration was selected. In both
cases the number of the month is blinking,
ready for date input.
Note: if the display shows “ERR” the calibration
procedure failed. Verify that the CAL CHECK
Standards HI 95729-11 Cuvets, A and B, are
free from fingerprints or dirt and that they are
inserted correctly.
DATE INPUT
• Keep READ/TIMER pressed to scroll to the
desired month number (01-12).
• When the correct month has been set, press
ZERO to confirm. Now the display will show
the day number blinking. Keep READ/TIMER
pressed to scroll to the desired day number
(01-31).
Note: it is possible to change from day to
month and vice versa by pressing ZERO.
or
TM
ABBREVIATIONS
°C:
degree Celsius
°F:
degree Fahrenheit
EPA:
Environmental Protection Agency (USA)
LCD:
Liquid Crystal Display
mg/L:
SPADNS:
milligrams per liter. mg/L is equivalent to ppm (part per million)
• When both the day and month have been
selected, hold CAL CHECK
store date and calibration values. The instrument
will show for one second “Stor”, to confirm
that the new calibration data has been accepted.
• The instrument will return automatically to the
measurement mode by displaying "729" on
the LCD.
4
for three seconds to
17
Page 5
CALIBRATION PROCEDURE
SPECIFICATIONS
Note it is possible to interrupt calibration procedure at any time by pressing ON/OFF.
Warning: do not validate or calibrate the instrument with standard solutions other than the Hanna
CAL CHECKTM Standards, otherwise erroneous results will be obtained. For accurate Validation and
Calibration results, please perform test at room temperature: 18 to 25°C (64.5 to 77.0°F).
CALIBRATION PROCEDURE
• Turn the meter on by pressing ON/OFF.
When the LCD displays “729”, it is ready.
• Enter the calibration mode by holding CAL CHECK
for three seconds.
• The date of the last calibration appears (e.g.:
month “01”, day “08”). “F.CAL” means that
the factory calibration is selected.
Note: at this point it is possible to reset the
instrument to restore FACTORY CALIBRATION,
see instructions, page 18.
• Place the CAL CHECKTM Standard HI 95729-11
Cuvet A into the holder and ensure that the
notch on the cap is positioned securely into
the groove.
or
Range0.00 to 2.00 mg/L
Resolution0.01 mg/L
Precision
Typical EMC Deviation±0.01 mg/L
Light SourceTungsten lamp with narrow band interference filter @ 575 nm
Light DetectorSilicon Photocell
MethodAdaptation of the EPA method 340.1 and Standard Methods for
Environment0 to 50°C (32 to 122°F); max 95% RH non-condensing
Battery Type1 x 9 volt
Auto-Shut off
Dimensions180 x 83 x 46 mm (7.1 x 3.3 x 1.8")
Weight290 g (10 oz.).
REQUIRED REAGENTS
CodeDescriptionQuantity/test
HI 93729-0Fluoride Reagent4 mL (2 mL x 2)
±0.03 mg/L @ 1.00 mg/L
the Examination of Water and Wastewater, 20th edition, SPADNS
method. The reaction between fluoride and the liquid reagent
causes a red tint in the sample.
After 10' of non-use in measurement mode;
after 1 hour of non-use in calibration mode.
PRECISION AND ACCURACY
• Press ZERO and “SIP” will blink on the display.
• After a few seconds the display will show
“-0.0-”. The meter is now zeroed and ready for
calibration.
• Remove the cuvet.
• Place the CAL CHECKTM Standard HI 95729-11
Cuvet B into the holder and ensure that the
notch on the cap is positioned securely into
the groove.
16
Precision is how closely repeated measurements
agree with each other. Precision is usually expressed
as standard deviation (SD).
Accuracy is defined as the nearness of a test result to
the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure explains
these definitions.
In a laboratory using a standard solution of 1.00
mg/L fluoride and a representative lot of reagents,
an operator obtained with a single instrument a
standard deviation of 0.03 mg/L.
5
Page 6
PRINCIPLE OF OPERATION
VALIDATION PROCEDURE
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by
atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the
physical-chemical characteristics of the substance according to the
Lambert-Beer Law:
-log
I
ελc d
/I
o
or
A ελc d
Where:
-log
=Absorbance (A)
I
/I
o
=intensity of incident light beam
I
o
=intensity of light beam after absorption
I
ελ=molar extinction coefficient at wavelength λ
c=molar concentration of the substance
d=optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound
from a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna's HI 95 series colorimeters is based on special subminiature
tungsten lamps and narrow-band interference filters to guarantee both high performance and
reliable results.
Use the validation procedure to ensure that the instrument is properly calibrated.
Warning: do not validate or calibrate the instrument with standard solutions other than the Hanna
CAL CHECKTM Standards, otherwise erroneous results will be obtained. For accurate Validation and
Calibration results, please perform test at room temperature: 18 to 25°C (64.5 to 77.0°F).
• Turn the meter on by pressing ON/OFF.
When the LCD displays “729”, it is ready.
• Place the CAL CHECKTM Standard HI 95729-11
Cuvet A into the holder and ensure that the
notch on the cap is positioned securely into
the groove.
• Press ZERO and “SIP” will blink on the
display.
• After a few seconds the display will
show “-0.0-”. The meter is now zeroed and
ready for validation.
• Remove the cuvet and place the CAL CHECK
TM
Standard HI 95729-11 Cuvet B into the
holder. Ensure that the notch on the cap is
positioned securely into the groove.
HI 95 series block diagram (optical layout)
6
• Press CAL CHECK and “SIP” will blink during
measurement.
• Wait for a few seconds and the display will
show the validation standard value.
The reading should be within specifications as reported on the CAL CHECK
TM
Certificate. If the value found is out of specifications, please check that the cuvets are free of
fingerprints, oil or dirt and repeat validation. If results are still found out of specifications
then recalibrate the instrument.
15
Standard
Page 7
• Replace the cap to the cuvet, and shake gently
a couple of times.
• Place the cuvet with the reacted sample into
the holder and ensure that the notch on the cap
is positioned securely into the groove.
• Hold READ/TIMER
for three seconds and the
display will show the countdown or,
alternatively, wait for two minutes.
• Press READ/TIMER and “SIP” will blink during
measurement.
• The instrument directly displays concentration
in mg/L (ppm) of fluoride on the Liquid Crystal
Display.
reacted
sam
pl
A microprocessor controlled special tungsten lamp emits radiation which is first optically
conditioned and beamed to the sample contained in the cuvet. The optical path is fixed by the
diameter of the cuvet. Then the light is spectrally filtered to a narrow spectral bandwidth, to
obtain a light beam of intensity
or
I
I
.
o
The photoelectric cell collects the radiation I that is not absorbed by the sample and converts
it into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
e
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the
actual measurement is performed.
The cuvet has a very important role because it is an optical element and thus requires particular
attention. It is important that both the measurement and the calibration (zeroing) cuvets are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvet for both. It is necessary that the surface of the cuvet is clean and not scratched. This to
avoid measurement interference due to unwanted reflection and absorption of light. It is
recommended not to touch the cuvet walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvet to prevent any contamination.
Note:
For wastewater or seawater samples, before
performing measurements, distillation is
required.
INTERFERENCES
Chlorine interferes at all levels: to eliminate Cl2 interference add to the 10 mL unreacted
sample, 1 drops of the optional HI 93703-53 Chlorine Remover, for each 2 mg/L of chlorine.
Negative interferences may be caused by:
Alkalinity (as CaCO3) above 5000 mg/LAluminum above 0.1 mg/L
• READ/TIMER: this is a bi-functional key, just press for making a measurement, or hold the
key for 3 seconds to start a pre-programmed countdown prior to measurement.
• CAL CHECK: this bi-functional key allows to perform validation of the instrument just pressing
the CAL CHECK key, or hold the key for 3 seconds to enter the calibration mode.
OPERATING MODES
• Measurement mode: default operation mode, enables both validation and measurement.
• Calibration mode: may be entered by keeping CAL CHECK pressed
for three seconds (the “CAL”
icon appears), it enables calibration of the instrument.
DISPLAY ELEMENTS DESCRIPTION
1) four digit main display area.
2) CAL: appears when the instrument operates in the calibration mode.
3) the Month and Day icons appear above the number of month and day when a date is
displayed.
4) the blinking “Battery” icon appears when the battery voltage is getting low.
GUIDE TO DISPLAY CODES
This prompt appears for 1 second each time the instrument is
turned on.
This indicates that the meter is in a ready state and zeroing can
be performed.
Sampling in Progress. This flashing prompt appears each time the
meter is performing a measurement.
• Replace the cap to the cuvet, and shake
gently a couple of times.
• Place the cuvet with the reacted deionized
water (blank) into the holder and ensure that
the notch on the cap is positioned securely
into the groove.
• Hold READ/TIMER
for three seconds and the
display will show the countdown or,
alternatively, wait for two minutes.
• Press ZERO and "SIP" will blink on the
display.
Wait for a few seconds and the display will
show "-0.0-". Now the meter is zeroed and
ready for measurement.
• Remove the blank from the instrument.
• Remove the cap from the cuvet and discard
the blank.
• Rinse the cuvet a couple of times with the
unreacted sample and discard it.
Turn upside down the empty cuvet and shake
it to remove excess of liquid.
• Fill the same cuvet with 10 mL of unreacted
sample.
For most accurate results, use of another
clean class A laboratory pipette is strongly
recommended.
Alternatively, fill the cuvet up to the 10 mL
mark.
• Add 2 mL of HI 93729-0 Fluoride Reagent
to the cuvet by use of the 2000 µL automatic
pipette.
For a correct use of the automatic pipette,
please follow the related Instruction Sheet.
blank
2mL
8
13
Page 9
• Do not let the reacted sample stand too long after reagent is added, or accuracy will be lost.
• It is possible to take multiple readings in a row, but it is recommended to take a new zero
use the same cuvet for zeroing and measurementuse the same cuvet for zeroing and measurement
reading for each sample and to
use the same cuvet for zeroing and measurement.
use the same cuvet for zeroing and measurementuse the same cuvet for zeroing and measurement
• After the reading it is important to discard immediately the sample, otherwise the glass
might become permanently stained.
• All the reaction times reported in this manual are referred to 20°C (68°F). As a general rule
of thumb, they should be doubled at 10°C (50°F) and halved at 30°C (86°F).
• In order to maximize accuracy, prior to a measurement follow the validation procedure to
be sure that the instrument is properly calibrated. If necessary, calibrate the instrument.
This indicates that the meter is in a zeroed state and measurement
can be performed.
Light over range: the cuvet is not inserted correctly and an excess
ambient light is reaching the detector. If the cover is properly
installed, then contact your dealer or the nearest Hanna Customer
Service Center.
The blinking "BAT" indicates that the battery voltage is getting low
and the battery needs to be replaced.
This indicates that the battery is dead and must be replaced. Once
this indication is displayed, the meter will lock up. Change the
battery and restart.
MEASUREMENT PROCEDURE
Turn the meter on by pressing ON/OFF and follow instruction manual to perform either the
MEASUREMENT, VALIDATION or CALIBRATION PROCEDURE.
MEASUREMENT PROCEDURE
• Turn the meter on by pressing ON/OFF.
• When the LCD displays “729”, it is ready.
• Fill one cuvet with 10 mL of deionized water.
For most accurate results, use of a class A
laboratory pipette is strongly recommended.
Alternatively, fill the cuvet up to the 10 mL
mark.
2mL
• Add 2 mL of HI 93729-0 Fluoride Reagent to
the cuvet by use of the 2000 µL automatic
pipette.
For a correct use of the automatic pipette,
please follow the related Instruction Sheet.
“Configuration”: this indicates that the meter has lost its
configuration. Contact your dealer or the nearest Hanna Customer
Service Center.
CALIBRATION MODE MESSAGES
The date of the last calibration appears on the LCD each time the
calibration mode is entered. If calibration is performed for the first
time, “F.CAL” appears.
This indicates that the factory calibration is selected.
The flashing digits indicate that:
1) the month can be set
2) the day can be set
“Storage”: this prompt appears for 1 second at the end of the
calibration procedure, to indicate that the calibration data has
been stored.
“Error”: the concentration of the calibration solution used is not
correct. Repeat the calibration procedure with the right standard
solution, and verify it is not expired. If the calibration procedure fails
again, contact your dealer or the nearest Hanna Customer Service
Center.
12
9
Page 10
ERROR MESSAGES
GENERAL TIPS FOR AN ACCURATE MEASUREMENT
a) on zero reading
This indicates that the zeroing procedure failed due to a low signalto-noise ratio. In this case press ZERO again.
“No Light”: the instrument cannot adjust the light level. Please check
that the sample does not contain any debris.
“Light Low”: there is not enough light to perform a measurement.
Please check the preparation of the zero cuvet.
“Light High”: there is too much light to perform a measurement.
Please check the preparation of the zero cuvet.
b) on sample reading
There is too much light for the sample measurement. Please check
if the right sample cuvet is inserted.
“Inverted”: the sample and the zero cuvet are inverted.
“Zero”: a zero reading was not taken. Follow the instruction in
the measurement procedure for zeroing the meter.
Under range. A blinking "0.00" indicates that the sample absorbs
less light than the zero reference. Check the procedure and make
sure you use the same cuvet for reference (zero) and measurement.
A flashing value of the maximum concentration indicates an
over range condition. The concentration of the sample is beyond
the programmed range: dilute the sample and re-run the test.
The instructions listed below should be carefully followed during testing to ensure best accuracy.
• For adding the exact amount of sample and deionized water to
the cuvet it is strongly recommended to use two class A
laboratory pipettes.
• Alternatively, fill the cuvet up to the 10 mL mark: the liquid in
the cuvet forms a convexity on the top; the bottom of this
convexity must be at the same level of the 10 mL mark.
• For dosing the reagent, we recommend to use the supplied
Hanna HI 731342 automatic pipette.
For a correct use of the Hanna automatic pipette, please follow
the related Instruction Sheet.
• Color or suspended matter in large amounts may cause
interference, therefore these should be removed by treatment
with active carbon and by prior distillation.
• It is important that the sample does not contain any debris.
This would corrupt the reading.
• In order to avoid reagent leaking and to obtain more accurate
measurements, it is recommended to close the cuvet first with
the supplied HDPE plastic stopperand then
with the black cap.
class A pipette
Hanna
automatic pipette
A flashing value lower than the maximum concentration indicates
a low signal-to-noise ratio condition. In this case accuracy of the
result is not guaranteed. Repeat the measurement procedure.
10
• Each time the cuvet is used, the cap must be tightened to the
same degree.
• Whenever the cuvet is placed into the measurement cell, it
must be dry outside, and completely free of fingerprints, oil or
dirt. Wipe it thoroughly with HI 731318 (tissue for wiping
cuvets, see chapter ACCESSORIES) or a lint-free cloth prior to
insertion.
11
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