Hanna Instruments HI83325 Instruction Manual

HI83325

Nutrient Analysis Photometer

INSTRUCTION MANUAL

Dear Customer,

Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for correct use of the

instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e‑mail us at

tech@hannainst.com or view our worldwide contact list at www.hannainst.com.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

1. PRELIMINARY EXAMINATION ............................................................................................

2. SAFETY MEASURES...................... ....................................................................................

3. SPECIFICATIONS ..............................................................................................................

4. DESCRIPTION..... .............................................................................................................

4.1. GENERAL DESCRIPTION.............................................................................................

4.2. PRECISION AND ACCURACY........................................................................................

4.3. FUNCTIONAL DESCRIPTION........................................................................................

4.4. PRINCIPLE OF OPERATION.........................................................................................

4.5. OPTICAL SYSTEM......................................................................................................

5. GENERAL OPERATIONS......... ............................................................................................

5.1. POWER CONNECTION AND BATTERY MANAGEMENT......................................................

5.2. GENERAL SETUP........................................................................................................

5.3. USING HANNA DIGITAL ELECTRODES...........................................................................

5.4. MODE SELECTION.....................................................................................................

5.5. LOGGING DATA.........................................................................................................

5.6. ADDING SAMPLE / USER NAMES TO LOG DATA............................................................

5.7. DATA MANAGEMENT..................................................................................................

5.8. CONTEXTUAL HELP....................................................................................................

6. NUTRIENT SAMPLES PREPARATION GUIDE......... ................................................................

6.1. INTRODUCTION TO PLANT NUTRIENTS........................................................................

6.2. PREPARING NUTRIENT SAMPLES FOR ANALYSIS..........................................................

6.3. PROCEDURE FOR DILUTION FACTOR: 5.......................................................................

6.4. PROCEDURE FOR DILUTION FACTOR: 10.....................................................................

6.5. PROCEDURE FOR DILUTION FACTOR: 50.....................................................................

6.6. REMOVING TURBIDITY AND COLOR.............................................................................

7. PHOTOMETER MODE......... ...............................................................................................

7.1. METHOD SELECTION.................................................................................................

7.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS..............................................

7.2.1. PROPER USE OF SYRINGE...................................................................................

7.2.2. PROPER USE OF DROPPER...................................................................................

7.2.3. PROPER USE OF POWDER PACKET.........................................................................

7.3. CUVETTE PREPARATION.............................................................................................

7.4. TIMERS AND MEASUREMENT FUNCTIONS...................................................................

7.5. CHEMICAL FORMULA / UNIT CONVERSIONS................................................................

7.6. METER VALIDATION / CAL CHECK................................................................................

7.7. ABSORBANCE MEASUREMENTS..................................................................................

TABLE OF CONTENTS

8. PROBE MODE................... ...............................................................................................

8.1. pH CALIBRATION......................................................................................................

8.2. pH CALIBRATION MESSAGES......................................................................................

8.3. pH MEASUREMENT...................................................................................................

8.4. pH MEASUREMENT MESSAGES / WARNINGS...............................................................

8.5. pH GLP.....................................................................................................................

8.6. pH ELECTRODE CONDITIONING AND MAINTENANCE.....................................................

9. METHOD PROCEDURES........ ............................................................................................

9.1. AMMONIA LOW RANGE..............................................................................................

TABLE OF CONTENTS

9.2. AMMONIA MEDIUM RANGE........................................................................................

9.3. AMMONIA HIGH RANGE.............................................................................................

9.4. CALCIUM..................................................................................................................

9.5. MAGNESIUM............................................................................................................

9.6. NITRATE...................................................................................................................

9.7. PHOSPHATE HIGH RANGE..........................................................................................

9.8. POTASSIUM.............................................................................................................

9.9. SULFATE..................................................................................................................

10. ERROR DESCRIPTIONS......... ..........................................................................................

11. STANDARD METHODS......... ...........................................................................................

12. ACCESSORIES................................................................................................................

12.1. REAGENT SETS.......................................................................................................

12.2. pH ELECTRODES.....................................................................................................

12.3. pH SOLUTIONS.......................................................................................................

12.4. OTHER ACCESSORIES..............................................................................................

13. ABBREVIATIONS............................................................................................................

RECOMMENDATIONS FOR USERS..........................................................................................

WARRANTY.........................................................................................................................

1. PRELIMINARY EXAMINATION

Remove the instrument and accessories from the packaging and examine it carefully to make sure that no damage has occurred during shipping. Notify your nearest Hanna Customer Service Center if damage is observed.

Each HI83325 is supplied in carrying case with:

• Sample Cuvette and Cap (4 pcs.)

• Cloth for Wiping Cuvettes

• Scissors

• USB Cable

• 5 Vdc Power Adapter

• Instruction Manual

• Quality Certificate

• 100 mL plastic graduated beaker with cap

• 170 mL plastic graduated beaker

Note: Save all packing material until you are sure that the instrument works correctly.

Any damaged or defective item must be returned in its original packing material with the supplied accessories.

• 3 mL plastic pipette

• 5 mL graduated syringe

• 60 mL graduated syringe

• graduated cylinder

• spoon

• funnel

• filter paper

• demineralizer bottle for 10 L of water

• activated carbon for 50 tests

PRELIMINARY EXAMINATION

2. SAFETY MEASURES

•The chemicals contained in the reagent kits may be hazardous if improperly handled.

•Read the Safety Data Sheets (SDS) before performing tests.

• Safety equipment: Wear suitable eye protection and clothing when required, and follow instructions carefully.

• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released vapors.

• Waste disposal: for proper disposal of reagent kits and reacted samples, contact a licensed waste disposal provider.

SAFETY MEASURESS

3. SPECIFICATIONS

Measurement Channels

SPECIFICATIONS

Absorbance

Temperature

Additional Specifications

3 x optical channels

1 x digital electrode channel (pH measurement) Range 0.000 to 4.000 Abs Resolution 0.001 Abs Accuracy ±0.003 Abs (at 1.000 Abs) Light Source light emitting diode Bandpass Filter Bandwidth 8 nm

Bandpass Filter Wavelength Accuracy ±1.0 nm

Light Detector silicon photocell Cuvette Types round, 24.6 mm diameter Number of Methods 12 Range ‑2.00 to 16.00 pH (± 1000.0 mV)* Resolution 0.01 pH (0.1 mV) Accuracy ±0.01 pH (±0.2 mV) (@ 25 °C / 77 °F) Temperature Compensation ATC (‑5.0 to 100.0 °C; 23.0 to 212.0 °F)*

Calibration

Electrode Intelligent pH / temperature electrode Range ‑20.0 to 120.0°C (‑4.0 to 248.0 °F) Resolution 0.1 °C (0.1 °F) Accuracy ±0.5 °C (±0.9 °F) (@ 25 °C / 77 °F) Logging 1000 readings (mixed photometer and electrode) Display 128 x 64 pixel B/W LCD with backlight USB‑A (Host) Functions mass‑storage host USB‑B (Device) Functions power input, mass‑storage device

Battery Life

Power Supply

Environment

2 points, eligible from 5 available buffers (4.01,

6.86, 7.01, 9.18, 10.01 pH)

> 500 photometer measurements, or 50 hours of

continuous pH measurement

5 Vdc USB 2.0 power adapter/type micro‑B connector

3.7 Vdc Li‑polymer rechargeable battery,

non‑serviceable

0 to 50 °C (32 to 122 °F);

0 to 95% RH, non‑serviceable

Dimensions 206 x 177 x 97 mm (8.1 x 7.0 x 3.8") Weight 1.0 kg (2.2 lbs.)

*Limits will be reduced to actual probe/sensor limits.

4. DESCRIPTION

4.1.GENERAL DESCRIPTION HI83325 multiparameter photometer is compact and versatile meter with two measurement

modes: Absorbance and pH/ mV. Absorbance mode include CAL Check feature and 12 different methods that cover a wide variety of applications, making it ideal for both benchtop and portable operation.

• Digital electrode input for pH measurements

• Certified CAL Check cuvettes to confirm meter functionality

• Dual purpose micro‑USB flash drive

• Li‑polymer rechargeable battery

• Auto‑off

• Absorbance mode

• User and sample name entry

• GLP features

4.2.PRECISION AND ACCURACY

Precision is how closely repeated measurements are to one another. Precision is usually expressed as standard deviation (SD). Accuracy is defined as the closeness of a test result to the true value. Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these definitions. For each method, the accuracy is expressed in the related measurement section.

DESCRIPTION

4.3. FUNCTIONAL DESCRIPTION

DESCRIPTION

1) Splash‑proof keypad

2) Liquid Crystal Display (LCD)

3) Indexing mark

4) Protective port covers

5) Light‑blocking cover panel

6) Cuvette holder

7) ON/OFF power button

8) 3.5 mm TRRS (jack) input for digital electrodes

9) Standard USB host connector for data transfer to a USB flash drive

10) Micro‑USB device connector for power or PC interface

Keypad Description

The keypad contains 12 direct keys and 3 functional keys with the following functions:

Press the functional keys to perform the function displayed above them on the LCD.

Press to access the list of photometer methods.

Press to move up in a menu or a help screen, to increment a set value, or to access second level functions.

Press to toggle between photometer and pH (electrode) mode.

Press to move left in a menu or to decrement a set value.

Press to move down in a menu or a help screen, to decrement a set value, or to access second level functions.

Press to move right in a menu or to increment a set value.

Press to access the setup screen.

Press to log the current reading.

Press to review saved logs.

DESCRIPTION

Press to exit the current screen.

Press to display the help screen.

ON/OFF power button.

4.4. PRINCIPLE OF OPERATION

Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical‑chemical characteristics of the substance according to the Lambert‑Beer Law:

‑log I/I

= el c d

c d

A = e

 = intensity of incident light beam

I = intensity of light beam after absorption e

l

= molar extinction coefficient at wavelength l c = molar concentration of the substance d = optical path through the substance

DESCRIPTION

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are constant. Photometric chemical analysis is based on specific chemical reactions between a sample and reagent to produce a light‑absorbing compound.

4.5. OPTICAL SYSTEM

Instrument Block Diagram

The internal reference system (reference detector) of the HI83325 photometer compensates for any drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero) measurement and sample measurement. LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher luminous efficiency, providing more light while using less power. They also produce little heat, which could otherwise affect electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps have poor blue/violet light output. Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to be received. The end result is higher measurement stability and less wavelength error. A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette imperfections and scratches, eliminating the need to index the cuvette.

5. GENERAL OPERATIONS

5.1. POWER CONNECTION AND BATTERY MANAGEMENT

The meter can be powered from an AC/DC adapter (included) or from the built‑in rechargeable battery. The meter will perform an auto‑diagnostic test when it is first powered on. During this test, the HANNA® logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display. The battery icon on the LCD will indicate the battery status:

‑ battery is charging from external adapter ‑ battery fully charged (meter connected to AC/DC adapter)

‑ battery capacity (no external adapter) ‑ battery near 0% (no external adapter)

‑ battery at 0% (no external adapter)

GENERAL OPERATIONS

To conserve battery, the meter will turn off automatically after 15 minutes of inactivity (30 minutes before a READ measurement). If a photometer measurement is on the screen, an auto‑log is created before shutdown.

5.2. GENERAL SETUP

Press SETUP key to enter in Setup menu, highlight desired option using and press Select.

CAL Check (Photometer Only)

Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are displayed on the screen. To start a new CAL Check press Check key and follow the prompts on the screen.

Temperature Unit (pH Only)

Option: °C or °F

Press the functional key to select the desired temperature unit.

Backlight

Values: 0 to 8

Press the Modify key to access the backlight intensity. Use the functional keys or the   keys to increase or decrease the value.

GENERAL OPERATIONS

Press the Accept key to confirm or ESC to return to the Setup menu without saving the new value.

Contrast

Values: 0 to 20

Press the Modify key to change the display’s contrast. Use the functional keys or the   keys to increase or decrease the value. Press the Accept key to confirm the value or ESC to return to the Setup menu without saving the new value.

Date / Time

Press the Modify key to change the date/time. Press the functional keys or the   keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the   keys to change the value. Press the Accept key to confirm or ESC to return to the Setup without saving the new date or time.

Time Format

Option: AM/PM or 24-hour Press the functional key to select the desired time format.

Date Format

Press the Modify key to change the Date Format. Use the   keys to select the desired format. Press the Select key to confirm or ESC to return to the Setup menu without saving the new format.

Decimal Separator

Option: Comma ( , ) or Period ( . ) Press the functional key to select the desired decimal separator. The decimal separator is used on the measurement screen and CSV files.

Language

Press the Modify key to change the Language. Use the   keys to select the desired language. Press Select to choose one of the 7 languages installed.

GENERAL OPERATIONS

Beeper

Option: Enable or Disable

When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error is detected. Press the functional key to enable/disable the beeper.

Instrument ID

Option: 0 to 999999

This option is used to set the instrument’s ID (identification number). Press the Modify key to access the instrument ID screen. Use the functional keys or the  keys to highlight the digit to be modified. Press the   keys in order to set the desired value. Press the Accept key to confirm the value or ESC to return to the Setup menu without saving the new value.

Meter Information

Press the Select key to view the model, serial number, firmware version and selected language. Press ESC to return to the Setup menu.

GENERAL OPERATIONS

Probe Information (pH mode only)

Press the Select key to view model number, serial number and firmware version for the connected probe. Press ESC to return to the Setup menu.

5.3. USING HANNA DIGITAL ELECTRODES

The HI83325 can be used to perform direct pH measurements by connecting a HANNA® digital pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the electrode to the 3.5 mm port marked with “EXT PROBE” located at the rear of the meter. If the meter is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.

5.4. MODE SELECTION

The HI83325 has two operational modes: Photometer Mode and Probe Mode. Photometer Mode enables on‑demand measurement of a cuvette using the integrated optical system. Photometric‑related functions, such as Method selection, Zero, Read, and Timers are available in this mode.

Probe Mode enables continuous measurement using a Hanna Digital Electrode connected to the

3.5 mm port. Probe‑related functions, such as calibration and GLP, are available in this mode. To switch between Photometer Mode and Probe Mode, use the

Note: The active mode cannot be switched while in menus, such as Setup, Recall, Method, etc.

button.

5.5. LOGGING DATA

The instrument features a data log function to help you keep track of all your analysis. The data log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the LOG and RECALL keys. Storing data: You can store only a valid measurement. Press LOG and the last valid measurement will be stored with date and time stamp.

5.6. ADDING SAMPLE / USER NAMES TO LOG DATA

A sample ID and user ID can be added to the saved log. Use the  keys to highlight the Sample ID or User ID then press Modify.

Text Entry

Sample ID and User ID care entered using the alphanumeric multi‑tapping keypad.

GENERAL OPERATIONS

Enter one character at a time by pressing the key with the assigned character repeatedly until the desired character is highlighted. For reference, a list of the characters available for the current key will be shown under the text box. The character will be entered after a two‑second delay or after another key is pressed.

Once all characters have been entered, press Accept to use the displayed text.

The following functions are available during Text Entry:

• Accept: Press to accept the current displayed text.

• Arrow: Press to delete the last character.

• Clear: Press to delete all characters.

Press to discard all changes and return to the previous screen.

GENERAL OPERATIONS

5.7. DATA MANAGEMENT

Viewing and deleting: You can view, export and delete the data by pressing the RECALL key. Use the  keys to scroll through the saved logs. Press Info to view additional information about the selected log.

Data Export:

Log data can be exported to a USB flash drive or to a PC. To access Data Export functions, press Recall then Export.

Use the   keys to select the desired export location. For export to USB Flash Drive, insert the USB Flash Drive into the dedicated port at the back of the meter labeled HOST USB, then follow the on‑screen prompts.

For export to PC, connect the meter to a PC using the supplied micro‑USB cable. Insert the cable into the port at the back of the meter labeled PC PWR. Follow the on‑screen prompts. When the meter says PC connected, use a file manager (such as Windows Explorer or Mac Finder) to move the file from the meter to the PC. The meter will appear as a removable disk.

Log data is exported as a single file containing all logged photometer and probe data. The file name is: “HI83325.csv”. The CSV file (Comma‑Separated Values) may be opened with a text editor or spreadsheet application.

5.8. CONTEXTUAL HELP HI83325 offers an interactive contextual help mode that assists the user at any time.

To access the help screen press HELP. The instrument will display additional information related to the current screen. To read all the available information, scroll the text using the   keys. To exit help mode press ESC key and the meter will return to the previous screen.

GENERAL OPERATIONS

6. NUTRIENT SAMPLES PREPARATION GUIDE

6.1. INTRODUCTION TO PLANT NUTRIENTS

The three elements that are mostly needed by the plants are nitrogen (N), phosphorus (P) and potassium (K). They are called the macronutrients while other elements, needed by plants in smaller amounts, are called microelements. In hydroponics, plants need a balanced nutritive solution, composed of macro and microelements.

Shortage or excess of even only one nutritive element may cause an imbalance in plant physiology and in the absorption of the other nutrients. Nutrients shortages may result in irregular plant growth, low resistance to diseases, scarce production both in quantity and quality, while nutrients excess may cause waste of fertilizer, pollution of the groundwater and the possible accumulation of dangerous substances in the crops produced.

NITROGEN

‑

Nitrogen (N) is mostly absorbed by plants as nitrates (NO ammonium (NH

). In hydroponics, an adequate ratio between the two forms is generally used in

) and, in smaller amount, in the form of

nutritive solutions.

NUTRIENT SAMPLES PREPARATION GUIDE

PRESENT IN proteins, enzymes, chlorophyll, hormones, vitamins, DNA and RNA

• is fundamental for plants in phase of growth

• promotes lengthening of trunks and sprouts

ACTION

SHORTAGE EFFECTS

EXCESS EFFECT

NUTRIENT SAMPLES PREPARATION GUIDE

PHOSPHORUS

• increases the production of foliage

• helps to absorb other nutrients (in particular phosphorus)

• assists a bigger production for both size and number of fruits

• slower growth

• smaller leaves

• yellowing of leaves

• smaller fruits

• premature ripening

• reduction in resistance to diseases and atmospheric agents

• increase of water demand (caused by an excessive production of leaves)

• bad quality of fruits

• delayed ripening

• reduction in potassium absorption

Phosphorus (P) has an important role in many fundamental biochemical and physiological

‑

processes. Plants take up phosphorus in the form of phosphate ion (PO

PRESENT IN DNA and RNA, ATP, ADP

• stimulates the roots growth

• stimulates blooming

ACTION

SHORTAGE EFFECTS

• stimulates fecundation and ripeness

• strengthens the plant tissues

• is necessary in the formation of seeds

• delayed ripening

• slower growth

• small leaves

• decrease of production (smaller fruits and difficult seeds formation)

• reduction of root system

EXCESS EFFECT

• premature ripening

• excess of fruit‑setting

• negative effects on the absorption of some microelements such as iron, zinc, boron and copper

POTASSIUM

Potassium (K) is essential in proteic synthesis. The problem of lack of potassium is quite frequent in calcareous soils.

Potassium is absorbed as K

NUTRIENT SAMPLES PREPARATION GUIDE

PRESENT IN

ACTION

SHORTAGE EFFECTS

EXCESS EFFECT

tissues responsible for the growth of plants (primary and secondary meristems), embryos and cell vacuole

• improves the quality of fruits and flowers

• gives more resistance both to frost and to diseases caused by fungi (increases the cuticular thickness)

• regulates the cellular turgidity (helps to regulate the osmotic processes and increases the resistance to dryness)

• regulates the stomatic opening and closing (it means a strong influence on transpiration and photosynthesis)

• slower growth

• smaller fruits, less colored and less preserved

• increase of transpiration

• less resistance to the cold

• reduced absorption of calcium and magnesium

• increase of water consumption

• increase of the substrate salinity

IRRIGATION WATER

In agricultural areas it is quite common to find altered values in the chemical composition of irrigation waters. The problem concerns mostly the high nitrate concentration, usually determined by excessive fertilization or irrational liquid manure spreading. The analysis of irrigation waters allows us to find out which are the substances present in major or minor quantity and to organize an advantageous fertilization plan.

For example, if the quaninty of water utilized for crop cultivation is 250 mm/ha (=2500000 L/ha) and the nitrate (NO

‑

) concentration is 150 mg/L (34 mg/L as nitrate‑nitrogen NO3‑N), soil receives

85 kg/ha of nitrogen. In choosing type and fertilizer to be used, it is important to consider this information, in order not to waste fertilizer nor to induce soil pollution.

NUTRIENTS SOLUTIONS

The nutrients requirements of the plant are determined by the type of plant, its age and the environmental conditions. The control of chemical composition of nutrients solutions given to the plants is an operation that allows a correct preparation of the fertilizer. In analyzing the solution it is typically necessary to perform a dilution, depending on the concentration of substances.

A dilution factor of 5 usually covers the analysis of residual solution in recycling systems. The nutritive elements are differently absorbed by the plants, hence the nutrient solution loses substances, becomes impoverished and must be enriched.

A dilution factor of 10 normally corresponds to the typical values of nutrients solutions. It is therefore possible to verify that the solution given to the plants contains the correct quantities of nutritive substances.

6.2. PREPARING NUTRIENT SAMPLES FOR ANALYSIS

Nutrient samples need proper preparation before they can be analyzed by photometric methods. The three most common problems are:

1. High concentration (samples contain too much nutrient for the analysis method)

NUTRIENT SAMPLES PREPARATION GUIDE

2. Turbidity (samples appear cloudy or hazy)

3. Color (samples have a colored tint from soil or impurities) High nutrient concentration is overcome by dilution of the sample by a known amount with

demineralized water. This is most often encountered when measuring the macro‑nutrients: ammonia, nitrate, phosphorus, and potassium. The following sections explain procedures for diluting samples by factors of 5, 10, and 50. The table below recommends the dilution procedure and the method to use based on the estimated nutrient concentration:

Recommended Procedures/Dilutions According to Nutrient Concentration:

NUTRIENT SAMPLES PREPARATION GUIDE

Parameter

Ammonia

Nitrate

Phosphorus

Potassium

Estimated Concentration

< 2.5 ppm NH

2.5 ‑ 9 ppm NH

9 ‑ 100 ppm NH

< 25 ppm NO

25 ‑ 130 ppm NO

130 ‑ 300 ppm NO

‑N

< 9 ppm P

‑

(< 27 ppm PO

9 ‑ 45 ppm P (27 ‑ 135 ppm PO

45 ‑ 100 ppm P (135 ‑ 300 ppm PO

)

‑

)

‑

)

< 18 ppm K

18 ‑ 90 ppm K

90 ‑ 180 ppm K

180 ‑ 1000 ppm K

Dilution Factor

No dilution

Method Selection

Ammonia LR

Ammonia MR

Ammonia HR

Nitrate

Phosphate HR

Potassium

Typical Usage

Irrigation Water

Recycled Nutrient Solution Fresh Nutrient Solution

Irrigation Water

Recycled Nutrient Solution

Fresh Nutrient Solution

Irrigation Water

Recycled Nutrient Solution

Fresh Nutrient Solution

Irrigation Water

Recycled Nutrient Solution

Fresh Nutrient Solution

The concentration of the micro‑nutrients (calcium, magnesium, sulfate) is low enough in most samples that dilution is typically not required. If necessary, a dilution procedure can be used for these parameters as well.

6.3. PROCEDURE FOR DILUTION FACTOR: 5

Note: For a more accurate dilution, use laboratory-grade glass pipettes and volumetric flasks.

• Use the graduate cylinder to measure exactly 20 mL of sample

• Remove the cap and fill the Demineralizer Bottle with tap water.

NUTRIENT SAMPLES PREPARATION GUIDE

• Replace the cap and shake gently for at least 2 minutes.

• Open the upper part of the Demineralizer Bottle cap and gently squirt the demineralized water into the cylinder, up to the 100 mL mark.

Note: The ion exchange resin contained in the Demineralizer Bottle is provided with an indicator substance. The indicator will change from green to blue when the resin has been exhausted and needs to be replaced.

• Pour the solution in the large 170 mL beaker, replace the cap and invert several times to mix.

• If the solution contains some turbidity or color, follow the procedure in 6.6. REMOVING TURBIDITY AND COLOR.

6.4. PROCEDURE FOR DILUTION FACTOR: 10

Note: For a more accurate dilution, use laboratory-grade glass pipettes and volumetric flasks.

• Add 10 mL of sample to the graduated cylinder using the 5 mL syringe (twice).

Note: To measure exactly 5 mL of sample with the syringe, push the plunger completely into the syringe and insert the tip into the sample. Pull the plunger out until the lower edge of the seal is on the 5 mL mark of the syringe.

NUTRIENT SAMPLES PREPARATION GUIDE

+ 53 hidden pages

Hanna Instruments HI83325 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual