Hanna Instruments HI83303 Instruction Manual

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HI83303

Aquaculture Photometer

INSTRUCTION MANUAL

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Dear Customer,

Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for correct use of the

instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e‑mail us at

tech@hannainst.com or view our worldwide contact list at www.hannainst.com.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

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1. PRELIMINARY EXAMINATION ............................................................................................

2. SAFETY MEASURES...................... ....................................................................................

3. SPECIFICATIONS ..............................................................................................................

4. DESCRIPTION..... .............................................................................................................

4.1. GENERAL DESCRIPTION.............................................................................................

4.2. PRECISION AND ACCURACY........................................................................................

4.3. FUNCTIONAL DESCRIPTION........................................................................................

4.4. PRINCIPLE OF OPERATION.........................................................................................

4.5. OPTICAL SYSTEM......................................................................................................

5. GENERAL OPERATIONS......... ............................................................................................

5.1. POWER CONNECTION AND BATTERY MANAGEMENT......................................................

5.2. GENERAL SETUP........................................................................................................

5.3. USING HANNA DIGITAL ELECTRODES...........................................................................

5.4. MODE SELECTION.....................................................................................................

5.5. LOGGING DATA.........................................................................................................

5.6. ADDING SAMPLE / USER NAMES TO LOG DATA............................................................

5.7. DATA MANAGEMENT..................................................................................................

5.8. CONTEXTUAL HELP....................................................................................................

6. PHOTOMETER MODE......... ...............................................................................................

6.1. METHOD SELECTION.................................................................................................

6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS..............................................

6.2.1. PROPER USE OF SYRINGE...................................................................................

6.2.2. PROPER USE OF DROPPER...................................................................................

6.2.3. PROPER USE OF POWDER PACKET.........................................................................

6.3. CUVETTE PREPARATION.............................................................................................

6.4. TIMERS AND MEASUREMENT FUNCTIONS...................................................................

6.5. CHEMICAL FORMULA / UNIT CONVERSIONS................................................................

6.6. METER VALIDATION / CAL CHECK................................................................................

6.7. ABSORBANCE MEASUREMENTS..................................................................................

7. PROBE MODE................... ...............................................................................................

7.1. pH CALIBRATION......................................................................................................

7.2. pH CALIBRATION MESSAGES......................................................................................

7.3. pH MEASUREMENT...................................................................................................

7.4. pH MEASUREMENT MESSAGES / WARNINGS...............................................................

7.5. pH GLP.....................................................................................................................

7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE.....................................................

TABLE OF CONTENTS

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8. METHOD PROCEDURES........ ........................................................................................

8.1. ALKALINITY...............................................................................................................

8.2. ALKALINITY, MARINE.................................................................................................

8.3. AMMONIA LOW RANGE..............................................................................................

8.4. AMMONIA MEDIUM RANGE........................................................................................

8.5. AMMONIA HIGH RANGE.............................................................................................

8.6. CALCIUM..................................................................................................................

8.7. CALCIUM, MARINE....................................................................................................

8.8. CHLORINE, FREE.......................................................................................................

TABLE OF CONTENTS

8.9. CHLORINE, TOTAL.....................................................................................................

8.10. COPPER LOW RANGE...............................................................................................

8.11. COPPER HIGH RANGE..............................................................................................

8.12. NITRATE................................................................................................................

8.13. NITRITE, MARINE ULTRA LOW RANGE.......................................................................

8.14. NITRITE LOW RANGE...............................................................................................

8.15. NITRITE HIGH RANGE..............................................................................................

8.16. OXYGEN, DISSOLVED...............................................................................................

8.17. pH........................................................................................................................

8.18. PHOSPHATE, MARINE ULTRA LOW RANGE.................................................................

8.19. PHOSPHATE LOW RANGE.........................................................................................

8.20. PHOSPHATE HIGH RANGE........................................................................................

9. ERROR DESCRIPTIONS......... ........................................................................................

10. STANDARD METHODS......... ...........................................................................................

11. ACCESSORIES................................................................................................................

11.1. REAGENT SETS.......................................................................................................

11.2. pH ELECTRODES.....................................................................................................

11.3. pH SOLUTIONS.....................................................................................................

11.4. OTHER ACCESSORIES..............................................................................................

12. ABBREVIATIONS............................................................................................................

RECOMMENDATIONS FOR USERS..........................................................................................

WARRANTY.........................................................................................................................

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1. PRELIMINARY EXAMINATION

Remove the instrument and accessories from the packaging and examine it carefully to make sure that no damage has occurred during shipping. Notify your nearest Hanna Customer Service Center if damage is observed.

Each HI83303 is supplied with:

• Sample Cuvette and Cap (4 pcs.)

• Cloth for Wiping Cuvettes

• Scissors

• USB Cable

• 5 Vdc Power Adapter

• Instruction Manual

• DO bottle (glass stopper bottle)

• Quality Certificate

Note: Save all packing material until you are sure that the instrument works correctly. Any damaged or defective item must be returned in its original packing material with the supplied accessories.

PRELIMINARY EXAMINATION SAFETY MEASURES

2. SAFETY MEASURES

•The chemicals contained in the reagent kits may be hazardous if improperly handled.

•Read the Safety Data Sheets (SDS) before performing tests.

• Safety equipment: Wear suitable eye protection and clothing when required, and follow instructions carefully.

• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released vapors.

• Waste disposal: for proper disposal of reagent kits and reacted samples, contact a licensed waste disposal provider.

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3. SPECIFICATIONS

Measurement Channels

SPECIFICATIONS

Absorbance

Temperature

Additional Specifications

5 x optical channels;

1 x digital electrode channel (pH measurement) Range 0.000 to 4.000 Abs Resolution 0.001 Abs Accuracy ±0.003 Abs (at 1.000 Abs) Light Source light emitting diode Bandpass Filter Bandwidth 8 nm

Bandpass Filter Wavelength Accuracy ±1.0 nm

Light Detector silicon photocell Cuvette Types round, 24.6 mm diameter Number of Methods 25 Range ‑2.00 to 16.00 pH (± 1000.0 mV)* Resolution 0.01 pH (0.1 mV) Accuracy ±0.01 pH (±0.2 mV) (@ 25 °C / 77 °F) Temperature Compensation ATC (‑5.0 to 100.0 °C; 23.0 to 212.0 °F)*

Calibration

Electrode Intelligent pH / temperature electrode Range ‑20.0 to 120.0°C (‑4.0 to 248.0 °F) Resolution 0.1 °C (0.1 °F) Accuracy ±0.5 °C (±0.9 °F) (@ 25 °C / 77 °F) Logging 1000 readings (mixed photometer and electrode) Display 128 x 64 pixel B/W LCD with backlight USB‑A (Host) Functions mass‑storage host USB‑B (Device) Functions power input, mass‑storage device

Battery Life

Power Supply

Environment

2 points, eligible from 5 available buffers (4.01,

6.86, 7.01, 9.18, 10.01 pH)

> 500 photometer measurements, or 50 hours of

continuous pH measurement

5 Vdc USB 2.0 power adapter/type micro‑B connector

3.7 Vdc Li‑polymer rechargeable battery,

non‑serviceable

0 to 50 °C (32 to 122 °F);

0 to 95% RH, non‑serviceable

Dimensions 206 x 177 x 97 mm (8.1 x 7.0 x 3.8") Weight 1.0 kg (2.2 lbs.)

*Limits will be reduced to actual probe/sensor limits.

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4. DESCRIPTION

4.1.GENERAL DESCRIPTION HI83303 multiparameter photometer is compact and versatile meter with two measurement

modes: Absorbance and pH/ mV. Absorbance mode include CAL Check feature and 25 different methods that cover a wide variety of applications, making it ideal for both benchtop and portable operation.

• Digital electrode input for pH measurements

• Certified CAL Check cuvettes to confirm meter functionality

• Dual purpose micro‑USB flash drive

• Li‑polymer rechargeable battery

• Auto‑off

• Absorbance mode

• User and sample name entry

• GLP features

4.2.PRECISION AND ACCURACY

Precision is how closely repeated measurements are to one another. Precision is usually expressed as standard deviation (SD). Accuracy is defined as the closeness of a test result to the true value. Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these definitions. For each method, the accuracy is expressed in the related measurement section.

DESCRIPTION

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4.3. FUNCTIONAL DESCRIPTION

DESCRIPTION

1) Splash‑proof keypad

2) Liquid Crystal Display (LCD)

3) Indexing mark

4) Protective port covers

5) Light‑blocking cover panel

6) Cuvette holder

7) ON/OFF power button

8) 3.5 mm TRRS (jack) input for digital electrodes

9) Standard USB host connector for data transfer to a USB flash drive

10) Micro‑USB device connector for power or PC interface

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Keypad Description

The keypad contains 12 direct keys and 3 functional keys with the following functions:

Press the functional keys to perform the function displayed above them on the LCD.

Press to access the list of photometer methods.

Press to move up in a menu or a help screen, to increment a set value, or to access second level functions.

Press to toggle between photometer and pH (electrode) mode.

Press to move left in a menu or to decrement a set value.

Press to move down in a menu or a help screen, to decrement a set value, or to access second level functions.

Press to move right in a menu or to increment a set value.

Press to access the setup screen.

Press to log the current reading.

Press to review saved logs.

DESCRIPTION

Press to exit the current screen.

Press to display the help screen.

ON/OFF power button.

4.4. PRINCIPLE OF OPERATION

Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical‑chemical characteristics of the substance according to the Lambert‑Beer Law:

‑log I/I

= el c d

c d

A = e

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 = intensity of incident light beam

I = intensity of light beam after absorption e

l

= molar extinction coefficient at wavelength l c = molar concentration of the substance d = optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the

DESCRIPTION

other factors are constant. Photometric chemical analysis is based on specific chemical reactions between a sample and reagent to produce a light‑absorbing compound.

4.5. OPTICAL SYSTEM

Instrument Block Diagram

The internal reference system (reference detector) of the HI83303 photometer compensates for any drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero) measurement and sample measurement. LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher luminous efficiency, providing more light while using less power. They also produce little heat, which could otherwise affect electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps have poor blue/violet light output. Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to be received. The end result is higher measurement stability and less wavelength error. A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette imperfections and scratches, eliminating the need to index the cuvette.

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5. GENERAL OPERATIONS

5.1. POWER CONNECTION AND BATTERY MANAGEMENT

The meter can be powered from an AC/DC adapter (included) or from the built‑in rechargeable battery. The meter will perform an auto‑diagnostic test when it is first powered on. During this test, the HANNA® logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display. The battery icon on the LCD will indicate the battery status:

‑ battery is charging from external adapter ‑ battery fully charged (meter connected to AC/DC adapter)

‑ battery capacity (no external adapter) ‑ battery near 0% (no external adapter)

‑ battery at 0% (no external adapter)

GENERAL OPERATIONS

To conserve battery, the meter will turn off automatically after 15 minutes of inactivity (30 minutes before a READ measurement). If a photometer measurement is on the screen, an auto‑log is created before shutdown.

5.2. GENERAL SETUP

Press SETUP key to enter in Setup menu, highlight desired option using and press Select.

CAL Check (Photometer Only)

Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are displayed on the screen. To start a new CAL Check press Check key and follow the prompts on the screen.

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Temperature Unit (pH Only)

Option: °C or °F

Press the functional key to select the desired temperature unit.

Backlight

Values: 0 to 8

Press the Modify key to access the backlight intensity. Use the functional keys or the   keys to increase or decrease the value.

GENERAL OPERATIONS

Press the Accept key to confirm or ESC to return to the Setup menu without saving the new value.

Contrast

Values: 0 to 20

Press the Modify key to change the display’s contrast. Use the functional keys or the   keys to increase or decrease the value. Press the Accept key to confirm the value or ESC to return to the Setup menu without saving the new value.

Date / Time

Press the Modify key to change the date/time. Press the functional keys or the   keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the   keys to change the value. Press the Accept key to confirm or ESC to return to the Setup without saving the new date or time.

Time Format

Option: AM/PM or 24-hour Press the functional key to select the desired time format.

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Date Format

Press the Modify key to change the Date Format. Use the   keys to select the desired format. Press the Select key to confirm or ESC to return to the Setup menu without saving the new format.

Decimal Separator

Option: Comma ( , ) or Period ( . ) Press the functional key to select the desired decimal separator. The decimal separator is used on the measurement screen and CSV files.

Language

Press the Modify key to change the Language. Use the   keys to select the desired language. Press Select to choose one of the 7 languages installed.

GENERAL OPERATIONS

Beeper

Option: Enable or Disable

When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error is detected. Press the functional key to enable/disable the beeper.

Instrument ID

Option: 0 to 999999

This option is used to set the instrument’s ID (identification number). Press the Modify key to access the instrument ID screen. Use the functional keys or the  keys to highlight the digit to be modified. Press the   keys in order to set the desired value. Press the Accept key to confirm the value or ESC to return to the Setup menu without saving the new value.

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Meter Information

Press the Select key to view the model, serial number, firmware version and selected language. Press ESC to return to the Setup menu.

GENERAL OPERATIONS

Probe Information (pH mode only)

Press the Select key to view model number, serial number and firmware version for the connected probe. Press ESC to return to the Setup menu.

5.3. USING HANNA DIGITAL ELECTRODES

The HI83303 can be used to perform direct pH measurements by connecting a HANNA® digital pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the electrode to the 3.5 mm port marked with “EXT PROBE” located at the rear of the meter. If the meter is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.

5.4. MODE SELECTION

The HI83303 has two operational modes: Photometer Mode and Probe Mode. Photometer Mode enables on‑demand measurement of a cuvette using the integrated optical system. Photometric‑related functions, such as Method selection, Zero, Read, and Timers are available in this mode.

Probe Mode enables continuous measurement using a Hanna Digital Electrode connected to the

3.5 mm port. Probe‑related functions, such as calibration and GLP, are available in this mode. To switch between Photometer Mode and Probe Mode, use the

Note: The active mode cannot be switched while in menus, such as Setup, Recall, Method, etc.

button.

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5.5. LOGGING DATA

The instrument features a data log function to help you keep track of all your analysis. The data log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the LOG and RECALL keys. Storing data: You can store only a valid measurement. Press LOG and the last valid measurement will be stored with date and time stamp.

5.6. ADDING SAMPLE / USER NAMES TO LOG DATA

A sample ID and user ID can be added to the saved log. Use the  keys to highlight the Sample ID or User ID then press Modify.

Text Entry

Sample ID and User ID care entered using the alphanumeric multi‑tapping keypad.

GENERAL OPERATIONS

Enter one character at a time by pressing the key with the assigned character repeatedly until the desired character is highlighted. For reference, a list of the characters available for the current key will be shown under the text box. The character will be entered after a two‑second delay or after another key is pressed.

Once all characters have been entered, press Accept to use the displayed text.

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The following functions are available during Text Entry:

• Accept: Press to accept the current displayed text.

• Arrow: Press to delete the last character.

• Clear: Press to delete all characters.

Press to discard all changes and return to the previous screen.

GENERAL OPERATIONS

5.7. DATA MANAGEMENT

Viewing and deleting: You can view, export and delete the data by pressing the RECALL key. Use the  keys to scroll through the saved logs. Press Info to view additional information about the selected log.

Data Export:

Log data can be exported to a USB flash drive or to a PC. To access Data Export functions, press Recall then Export.

Use the   keys to select the desired export location. For export to USB Flash Drive, insert the USB Flash Drive into the dedicated port at the back of the meter labeled HOST USB, then follow the on‑screen prompts.

For export to PC, connect the meter to a PC using the supplied micro‑USB cable. Insert the cable into the port at the back of the meter labeled PC PWR. Follow the on‑screen prompts. When the meter says PC connected, use a file manager (such as Windows Explorer or Mac Finder) to move the file from the meter to the PC. The meter will appear as a removable disk.

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Log data is exported as a single file containing all logged photometer and probe data. The file name is: “HI83303.csv”. The CSV file (Comma‑Separated Values) may be opened with a text editor or spreadsheet application.

5.8. CONTEXTUAL HELP HI83303 offers an interactive contextual help mode that assists the user at any time.

To access the help screen press HELP. The instrument will display additional information related to the current screen. To read all the available information, scroll the text using the   keys. To exit help mode press ESC key and the meter will return to the previous screen.

GENERAL OPERATIONS

6. PHOTOMETER MODE

6.1. METHOD SELECTION

In order to select the desired method press the METHOD key and a screen with the available methods will appear. Press the  keys to highlight the desired method. Press Select.

After the desired method is selected, follow the procedure described in the related section. Before performing a method read all the instructions carefully.

PHOTOMETER MODE

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6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS

6.2.1. PROPER USE OF SYRINGE

(a) Push the plunger completely into the syringe and insert the tip

into the solution.

(b) Pull the plunger up until the lower edge of the seal is exactly on

the mark for the desired volume.

PHOTOMETER MODE

sure that no drops are hanging on the tip of the syringe. Then, keeping the syringe in vertical position above the cuvette, push the plunger down into the syringe, the desired volume has been delivered into the cuvette.

6.2.2. PROPER USE OF DROPPER

(a) For reproducible results, tap the dropper on the table several times and wipe the outside of the

tip with a cloth.

(b) Always keep the dropper bottle in a vertical position while dosing the reagent.

6.2.3. PROPER USE OF POWDER PACKET

(a) Use scissors to open the powder packet (b) Push the edges of the packet to form a spout (c) Pour out the content of the packet.

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6.3. CUVETTE PREPARATION

Proper mixing is very important for reproducibility of the measurements. The proper mixing technique for each method is listed in the method procedure. (a) Invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical

position. Turn the cuvette upside‑down and wait for all of the solution to flow to the cap end, then return the cuvette to the upright vertical position and wait for all of the solution to flow to the cuvette bottom. This is one inversion. The correct speed for this mixing technique is 10‑15 complete inversions in 30 seconds.

This mixing technique is indicated with “invert to mix” and the following icon:

(b) Shaking the cuvette, moving the cuvette up and down. The movement may be gentle or

vigorous. This mixing method is indicated with “shake gently” or “shake vigorously”, and one of the following icons:

PHOTOMETER MODE

shake gently shake vigorously

In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied HDPE plastic stopper black cap. Whenever the cuvette is placed into the measurement holder, it must be dry outside and free of fingerprints, oil or dirt. Wipe it thoroughly with HI731318 or a lint‑free cloth prior to insertion. Shaking the cuvette can generate bubbles in the sample, causing higher readings. To obtain accurate measurements, remove such bubbles by swirling or by gently tapping the cuvette.

and then the

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Do not let the reacted sample stand too long after reagent is added. For best accuracy, respect the timings described in each specific method.

It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for each sample and to use the same cuvette for zeroing and measurement when possible. Discard the sample immediately after the reading is taken, or the glass might become permanently stained. All the reaction times reported in this manual are at 25 °C (77 °F). In general, the reaction time should be increased for temperatures lower

PHOTOMETER MODE

than 20 °C (68 °F), and decreased for temperatures higher than 25 °C (77 °F).

Interference

In the method measurement section the most common interferences that may be present in a typical water sample have been reported. It is possible that a particular application could introduce other compounds that will also interfere.

6.4. TIMERS AND MEASUREMENT FUNCTIONS

Each method requires a different preparation procedure, reaction times, sample preparations, etc. If a timer or timers are necessary for proper sample preparation, the Timer key will be available.

To use a reaction timer, press the Timer key.

The default timer will start immediately. To stop and reset the timer, press Stop.

If the selected method requires more than one timer, the meter will automatically select each timer in the appropriate order. To bypass the default order, you may press the desired key to activate a different timer (only while the current timer is stopped). Press Continue to start the active timer.

For some methods, the timer is only necessary after a Zero measurement has been performed. In this case, the timer key will only be available after the Zero measurement has been performed.

If the method requires a Zero or Read measurement after a timer has expired, the meter will automatically perform the appropriate action. Follow the instructions in the Method Procedure.

To perform a Zero or Read measurement, insert the appropriate prepared cuvette, then press the Zero or Read key. A Zero measurement must be conducted before Read measurements.

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6.5. CHEMICAL FORMULA / UNIT CONVERSION

Chemical formula/unit conversion factors are pre‑programmed into the instrument and are method specific. In order to view the displayed result in the desired chemical formula press  keys to access the second level function and then press the Chem Frm key to toggle between the available chemical formulas for the selected method.

6.6. METER VALIDATION / CAL CHECK

WARNING: Do not validate the meter with standard solutions other than the HANNA

CAL Check

Standards. For accurate validation results, please perform tests at room temperature (18 to 25 °C;

64.5 to 77.0 °F).

Validation of the HI83303 involves absorbance measurements of certified HANNA

CAL Check Standards (see “Accessories”). The “CAL Check” screen guides the user through the measurement of each CAL Check Standard and applies the factory calibration corrections to each measurement. The

HI83303 stores the results of the most recent CAL Check measurements which may be viewed on

the “CAL Check” screen. Compare these results with the values printed on the Certificate provided with each HANNA® CAL Check Standards kit.

To perform a validation:

PHOTOMETER MODE

1. Press Setup button.

2. Highlight CAL Check, then press Select.

3. Follow the prompts on the screen. The meter will prompt to

measure each cuvette provided in the HANNA

CAL Check

Standards kit. To abort the process at any time, press ESC button.

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4. Press ESC to return in Setup menu.

6.7. ABSORBANCE MEASUREMENTS

Raw absorbance measurements may be performed on the HI83303 for personal or diagnostic purposes. For example, you may monitor the stability of a reagent blank by occasionally measuring its absorbance versus deionized water.

To measure the raw absorbance of a prepared sample:

PHOTOMETER MODE

1. Enable “Photometer Mode” if necessary by pressing the MODE key.

2. Press the METHOD key.

3. Highlight the appropriate Absorbance method (according to the wavelength to be used), then

press Select. Absorbance methods are located at the bottom of the method list.

4. Prepare the sample cuvette according to the method.

5. Insert a cuvette filled with deionized water, then press Zero.

6. Insert the prepared sample cuvette, then press Read.

WARNING: Never use Absorbance methods for validation using HANNA factory calibration corrections for CAL Check cuvettes are applied while in CAL Check mode only!

CAL Check cuvettes. The

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7. PROBE MODE

7.1. pH CALIBRATION

Press MODE to enter in pH/ mV measurement mode.

Press Calibrate to access electrode calibration functions.

Calibration Mode

While in pH Calibration Mode, the display will show the current pH reading, the current temperature reading, the current selected buffer, and the buffer number (“Buffer: 1” for the 1st buffer, “Buffer: 2” for the 2nd buffer).

The following functions are available in pH Calibration Mode:

• Clear: Press to clear the current calibration from the probe.

PROBE MODE

• Confirm: Press to accept the current calibration point. Only

available if the measurement is stable and within the limits for the selected buffer.

Press to cycle through the list of available buffers: 4.01, 6.86, 7.01, 9.18, 10.01 pH.

Press to exit calibration and return to pH Measurement Mode.

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Preparation

Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences. For accurate calibration and to minimize cross‑contamination, use two beakers for each buffer solution: one for rinsing the electrode and one for calibration. If you are measuring in the acidic range, use pH 7.01 or 6.86 as the first buffer and pH 4.01 as the second

PROBE MODE

buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as the first buffer and pH 10.01 or 9.18 as the second buffer.

Procedure

Calibration can be performed using one or two calibration buffers. For more accurate measurements, a two‑point calibration is recommended. Submerse the pH electrode approximately 3 cm (1¼”) into a buffer solution and stir gently. From the Probe Measurement screen, press the Calibrate key to begin the calibration process.

When the reading is stable and close to the selected buffer, the Confirm key will become available. Press Confirm to accept and store the calibration point.

The meter will now prompt for the second buffer (“Buffer: 2”). To use only a one‑point calibration, press to the probe and return to Measurement mode. To continue calibrating with a second buffer, rinse and submerse the pH electrode approximately 3 cm (1¼”) into the second buffer solution and stir gently. If necessary, press

to exit calibration mode at this time. The meter will store the calibration information

keys to select a different buffer value.

When the reading is stable and close to the selected buffer, the Confirm key will become available. Press Confirm to accept and store the second calibration point.

The meter will store the two‑point calibration information to the probe and return to Measurement mode. The list of calibrated buffers will appear at the bottom of the screen.

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7.2. pH CALIBRATION MESSAGES

Clean Probe:

The "Clean Probe" message indicates poor electrode performance (offset out of accepted window, or slope under the accepted lower limit). Often, cleaning the probe will improve the pH electrode's response. See pH Electrode Conditioning and Maintenance for details. Repeat calibration after cleaning.

Check Probe & Buffer:

The “Check Probe & Buffer” message appears when there is a large difference between the pH measurement and the selected buffer value, or the electrode slope is outside of the accepted slope limit. You should check your probe and confirm the correct buffer selection. Cleaning may also improve this response.

Wrong Temperature:

The buffer temperature is too extreme for the selected buffer value.

7.3. pH MEASUREMENT

The HI83303 can be used to perform direct pH measurements by connecting a HANNA pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter. If the meter is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.

digital

PROBE MODE

While taking pH probe measurements, the following functions are available:

• Calibrate: Press to access electrode calibration functions.

• GLP: Press to review the last calibration information, including date/time, buffers used, slope,

and offset.

• Range: Press to switch between “pH” units and “mV” units.

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Press to switch to Photometer mode.

Press to access the meter’s Setup menu.

Press to log the current measurement.

PROBE MODE

For high accuracy it is recommended to calibrate your electrode often. pH electrodes should be recalibrated at least once per week, but daily calibration is recommended. Always recalibrate after cleaning an electrode. See page 23 for more information on pH calibration.

To take pH measurements:

• Remove the protective cap and rinse the electrode with water.

• Collect some sample in a clean, dry beaker.

• Preferably, rinse the electrode with a small amount of sample. Discard the rinse.

• Submerse the electrode tip approximately 3 cm (1¼”) into the sample to be tested and stir the sample gently. Make sure the electrode junction is completely submersed.

• Allow time for the electrode to stabilize in the sample. When the reading is stable.

If measurements are taken successively in different samples, it is recommended to rinse the electrodes thoroughly with deionized or distilled water and then with some of the next sample to prevent cross‑contamination.

pH measurements are affected by temperature. HANNA temperature sensor and automatically calculate corrected pH values. The measured temperature is displayed on the screen with the pH measurements.

Press to review the meter’s log history.

Press to view contextual help information.

symbol disappears, your

Digital pH electrodes include a built‑in

Page 27

7.4. pH MEASUREMENT MESSAGES / WARNINGS

No Probe:

No probe is connected or the probe is broken.

Connecting:

The meter has detected a probe and is reading the probe configuration and calibration information.

Incompatible Probe:

The connected probe is not compatible with this device.

Incompatible Calibration:

The probe’s current calibration is not compatible with this meter. The calibration must be cleared to use this probe.

Exceeded Probe Range:

The pH and/or temperature measurement exceed the specifications of the probe. The affected measurement value(s) will be flashing.

Broken Temperature Sensor:

The temperature sensor inside the probe is broken. Temperature compensation will revert to a fixed value of 25 °C (77 °C).

PROBE MODE

Cal Due:

The probe has no calibration. See section Probe Calibration.

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7.5. pH GLP

Good Laboratory Practice (GLP) refers to a quality control function used to ensure uniformity and consistency of sensor calibrations and measurements. To view the GLP information, press the GLP key from the Probe Measurement screen.

PROBE MODE

The pH GLP screen displays the following information about the last pH calibration:

• Date and time of the last calibration

• List of buffers used in the last calibration

• Calculated slope and offset

• Press ESC to return in measurement mode.

Page 29

7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE

Remove the protective cap of the pH electrode. DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode. These bubbles can be removed by “shaking down” the electrode as you would do with a glass thermometer. If the bulb and/or junction is dry, soak the electrode in HI70300 or HI80300 storage solution for at least one hour.

PROBE MODE

For refillable electrodes: If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI7082 or

HI8082 3.5M KCl Electrolyte Solution for double junction electrodes.

Unscrew the fill hole cover during measurements so the liquid reference junction maintains an outward flow of electrolyte.

Measurement

Rinse the electrode tip with distilled water. Submerse the tip 3 cm (1¼”) in the sample and stir gently for a few seconds. For a faster response and to avoid cross‑contamination of the samples, rinse the electrode tip with a few drops of the solution to be tested, before taking measurements.

Page 30

Storage Procedure

To minimize clogging and ensure a quick response time, the glass bulb and the junction should be kept moist and not allowed to dry out.

Replace the solution in the protective cap with a few drops of HI70300 or HI80300 Storage Solution or, in its absence, Filling Solution (HI7082 or HI8082 for double junction electrodes).

PROBE MODE

Follow the preparation procedure before taking measurements.

Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.

Periodic Maintenance

Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable or cracks on the electrode stem or bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the electrode. Rinse off any salt deposits with water. For refillable electrodes: Refill the reference chamber with fresh electrolyte (HI7082 or HI8082 for double junction electrodes). Allow the electrode to stand upright for 1 hour. Follow the Storage Procedure above.

Cleaning Procedure

Use diagnostic messages to aid pH electrode troubleshooting. Several cleaning solutions are available:

• General – Soak in Hanna HI7061 or HI8061 General Cleaning Solution for approximately ½ hour.

• Protein –Soak in Hanna HI7073 or HI8073 Protein Cleaning Solution for 15 minutes.

• Inorganic – Soak in Hanna HI7074 Inorganic Cleaning Solution for 15 minutes.

• Oil/grease – Rinse with Hanna HI7077 or HI8077 Oil and Fat Cleaning Solution.

Note: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel- filled electrodes) and soak the electrode in HI70300 or HI80300 Storage Solution for at least 1 hour before taking measurements.

Temperature Correlation For pH Sensitive Glass

Verify the temperature range by reading the limits on electrodes cap. The pH electrode’s life also depends on the temperature that is used. If constantly cycled between two temperatures, the life of the electrode is drastically reduced.

Page 31

8. METHOD PROCEDURES

8.1. ALKALINITY

SPECIFICATIONS

Range 0 to 500 mg/L (as CaCO3) Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method.

REQUIRED REAGENTS Code Description Quantity

HI775S Alkalinity Reagent 1 mL HI93755‑53 Chlorine Removal Reagent 1 drop

REAGENT SETS

HI775‑26 Reagents for 25 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Alkalinity method using the procedure described in the

Method Selection section (see page 17).

ALKALINITY

10 mL

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

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• Remove the cuvette.

Note: Any chlorine present in the sample will interfere with the reading. To remove the chlorine interference

ALKALINITY

add one drop of HI93755-53 Chlorine Removal Reagent to the unreacted sample.

• Add 1mL of HI775S Alkalinity Reagent to the sample using a 1 mL syringe.

• Replace the cap and invert 5 times.

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L calcium carbonate

(CaCO

Page 33

8.2. ALKALINITY, MARINE

SPECIFICATIONS

Range 0 to 300 mg/L (as CaCO3) Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method.

REQUIRED REAGENTS Code Description Quantity

HI755S Alkalinity Reagent 1 mL

REAGENT SETS

HI755‑26 Reagents for 25 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Alkalinity Marine method using the procedure described in the Method Selection section (see page 17).

ALKALINITY, MARINE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

10 mL

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• Remove the cuvette.

• Add 1 mL of HI755S Alkalinity Reagent to the sample using a 1mL syringe.

ALKALINITY, MARINE

• Replace the cap and gently invert 5 times.

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L as calcium

carbonate (CaCO

• Press  or  to access the second level functions.

• Press Chem Frm key to convert the result to degree KH (dKH).

• Press  or  to return to the measurement screen.

Page 35

8.3. AMMONIA LOW RANGE

SPECIFICATIONS

Range 0.00 to 3.00 mg/L (as NH3‑N) Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426 Nessler method.

REQUIRED REAGENTS Code Description Quantity

HI93700A‑0 Ammonia Low Range Reagent A 4 drops HI93700B‑0 Ammonia Low Range Reagent B 4 drops

REAGENT SETS

HI93700‑01 Reagents for 100 tests HI93700‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Ammonia LR method using the procedure described in the Method Selection section (see page 17).

10 mL

AMMONIA LOW RANGE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

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• Add 4 drops of HI93700A‑0 Ammonia Low Range Reagent A. Replace the cap and mix the solution.

• Add 4 drops of HI93700B‑0 Ammonia Low Range Reagent B.

AMMONIA LOW RANGE

Replace the cap and mix the solution.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH3-N).

Page 37

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium

(NH

• Press  or  to return to the measurement screen.

INTERFERENCE

Interference may be caused by: Acetone Alcohols Aldehydes Glycine Hardness above 1 g/L Iron Organic chloramines Sulfide Various aliphatic and aromatic amines

AMMONIA LOW RANGE

Page 38

8.4. AMMONIA MEDIUM RANGE

SPECIFICATIONS

Range 0.00 to 10.00 mg/L (as NH Resolution 0.01 mg/L Accuracy ±0.05 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler method.

REQUIRED REAGENTS Code Description Quantity

HI93715A‑0 Ammonia Medium Range Reagent A 4 drops

AMMONIA MEDIUM RANGE

HI93715B‑0 Ammonia Medium Range Reagent B 4 drops

REAGENT SETS

HI93715‑01 Reagents for 100 tests HI93715‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Ammonia MR method using the procedure described in the Method Selection section (see page 17).

‑N)

10 mL

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

Page 39

• Remove the cuvette.

• Add 4 drops of HI93715A‑0 Ammonia Medium Range Reagent A. Replace the cap and mix the solution.

• Add 4 drops of HI93715B‑0 Ammonia Medium Range Reagent B. Replace the cap and mix the solution.

• Reinsert the cuvette into the instrument and close the lid.

-N).

AMMONIA MEDIUM RANGE

• Press or to access the second level functions.

Page 40

• Press the Chem Frm key to convert the result in mg/L of ammonia (NH3) and ammonium

(NH

• Press  or  to return to the measurement screen.

INTERFERENCES

AMMONIA MEDIUM RANGE

Interference may be caused by: Acetone Alcohols Aldehydes Glycine Hardness above 1 g/L Iron Organic chloramines Sulfide Various aliphatic and aromatic amines

Page 41

8.5. AMMONIA HIGH RANGE

SPECIFICATIONS

Range 0.0 to 100.0 mg/L (as NH3‑N) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler method.

REQUIRED REAGENTS Code Description Quantity

HI93733A‑0 Ammonia High Range Reagent A 4 drops HI93733B‑0 Ammonia High Range Reagent B 9 mL

REAGENT SETS

HI93733‑01 Reagents for 100 tests HI93733‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Ammonia HR method using the procedure described in the Method Selection section (see page 17).

AMMONIA HIGH RANGE

• Add 1mL of unreacted sample to the cuvette using 1mL syringe.

• Use the pipette to fill the cuvette up to the 10 mL mark with HI93733B‑0 Ammonia High Range Reagent B. Replace the cap and mix the solution.

• Place the cuvette into the holder and close the lid.

1 mL

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• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

AMMONIA HIGH RANGE

• Add 4 drops of HI93733A‑0 Ammonia High Range Reagent A. Replace the cap and swirl the solution.

• Reinsert the cuvette into the instrument and close the lid.

-N).

• Press  or  to access the second level functions.

Page 43

•Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium

(NH

•Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Acetone Alcohols Aldehydes Glycine Hardness above 1 g/L Iron Organic chloramines Sulfide Various aliphatic and aromatic amines

AMMONIA HIGH RANGE

Page 44

8.6. CALCIUM

SPECIFICATIONS

Range 0 to 400 mg/L (as Ca2+)

CALCIUM

Resolution 1 mg/L Accuracy ±10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Oxalate method.

REQUIRED REAGENTS Code Description Quantity

‑ Buffer Reagent 4 drops HI93752A‑Ca Calcium Reagent A 7 mL HI93752B‑Ca Calcium Reagent B 1 mL

REAGENT SETS

HI937521‑01 Reagents for 50 tests HI937521‑03 Reagents for 150 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Calcium method using the procedure described in the Method Selection section (see page 17).

• Add 3 mL of unreacted sample to the cuvette using the 5 mL syringe.

3 mL of sample

• Use the pipette to fill the cuvette up to the 10 mL mark with the HI93752A‑Ca Calcium Reagent A.

• Add 4 drops of Buffer Reagent.

Page 45

• Replace the cap and invert several times to mix.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

• Add 1 mL of HI93752B‑Ca Calcium Reagent B to the sample using the 1 mL syringe. Invert the cuvette 10 times to mix (about 15 seconds).

CALCIUM

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes.

Page 46

• After waiting 5 minutes, invert again the cuvette 10 times to mix (about 15 seconds).

CALCIUM

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca

INTERFERENCES

Interferences may be caused by: Acidity (as CaCO

) above 1000 mg/L

Alkalinity (as CaCO3) above 1000 mg/L Magnesium (Mg2+) above 400 mg/L

Page 47

8.7. CALCIUM, MARINE

SPECIFICATIONS

Range 200 to 600 mg/L (as Ca2+) Resolution 1 mg/L Accuracy ±6% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Zincon method.

REQUIRED REAGENTS Code Description Quantity

HI7581 Calcium Reagent A 1 mL HI7582 Calcium Reagent B 1 packet

REAGENT SETS

HI758‑26 Reagents for 25 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Calcium Marine method using the procedure described in the Method Selection section (see page 17).

CALCIUM, MARINE

• Add 1 mL of HI7581 Calcium Reagent A to the cuvette using a 1 mL syringe.

• Use the plastic pipette to fill the cuvette to the 10 mL mark with deionized water and replace the cap. Invert 3‑5 times to mix.

• Place the cuvette into the holder and close the lid.

10 mL

Page 48

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

CALCIUM, MARINE

• Remove the cuvette.

• Use the minipipette to add 0.1 mL of sample to the cuvette.

• Add one packet of HI7582 Calcium Reagent B. Replace the cap and shake vigorously for 15 seconds or until the powder is completely dissolved. Allow air bubbles to dissipate for 15 seconds before taking a reading.

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca

Page 49

8.8. CHLORINE, FREE

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD method 330.5.

REQUIRED REAGENTS

POWDER:

Code Description Quantity

HI93701‑0 Free Chlorine Reagent 1 packet

LIQUID:

Code Description Quantity

HI93701A‑F Free Chlorine Reagent A 3 drops HI93701B‑F Free Chlorine Reagent B 3 drops

REAGENT SETS

HI93701‑F Reagents for 300 tests (liquid) HI93701‑01 Reagents for 100 tests (powder) HI93701‑03 Reagents for 300 tests (powder)

For other accessories see page 87.

)

CHLORINE, FREE

MEASUREMENT PROCEDURE

• Select the Chlorine (Free) method using the procedure described in the Method Selection section (see page 17).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

10 mL

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• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

CHLORINE, FREE

• Remove the cuvette.

POWDER REAGENT PROCEDURE

• Add the content of one packet of HI93701‑0 Free Chlorine Reagent. Replace the cap and shake gently for 20 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or alternatively, wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of chlorine (Cl

LIQUID REAGENT PROCEDURE

• To an empty cuvette add 3 drops of HI93701A‑F Free Chlorine Reagent A and 3 drops of HI93701B‑F Free Chlorine Reagent B.

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• Swirl gently to mix.

CHLORINE, FREE

• Add 10 mL of unreacted sample (up to the mark).

10 mL

Replace the cap and shake gently.

• Insert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of chlorine (Cl

Note: Free and Total Chlorine have to be measured separately with fresh sample following the related procedure if both values are desired.

INTERFERENCES

Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO

, shake the sample for

approximately 2 minutes after adding the powder reagent. If the water used for this procedure has an alkalinity value greater than 250 mg/L CaCO3 or acidity value greater than 150 mg/L CaCO3, the color of the sample may develop only partially, or may rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

Page 52

8.9. CHLORINE, TOTAL

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cl2) Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD method 330.5.

CHLORINE, TOTAL

REQUIRED REAGENTS

POWDER:

Code Description Quantity

HI93711‑0 Total Chlorine Reagent 1 packet

LIQUID:

Code Description Quantity

HI93701A‑T Total Chlorine Reagent A 3 drops HI93701B‑T Total Chlorine Reagent B 3 drops HI93701C‑T Total Chlorine Reagent C 1 drop

REAGENT SETS

HI93701‑T Reagents for 300 tests (liquid) HI93711‑01 Reagents for 100 total tests (powder) HI93711‑03 Reagents for 300 total tests (powder)

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Chlorine (Total) method using the procedure described in the Method Selection section (see page 17).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

10 mL

Page 53

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

POWDER REAGENT PROCEDURE

• Add 1 packet of HI93711‑0 Total Chlorine Reagent. Replace the cap and shake gently for 20 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of chlorine (Cl

CHLORINE, TOTAL

LIQUID REAGENT PROCEDURE

• To an empty cuvette add 3 drops of HI93701A‑T Total Chlorine Reagent A, 3 drops of HI93701B‑T Total Chlorine Reagent B, and 1 drop of

HI93701C‑T Total Chlorine Reagent C. Swirl gently

to mix.

Page 54

• Add 10 mL of unreacted sample (up to the mark). Replace the cap and shake gently.

10 mL

CHLORINE, TOTAL

• Insert the cuvette into the instrument and close the lid.

Note: Free and Total Chlorine have to be measured separately with fresh unreacted samples following the related procedure if both values are desired.

INTERFERENCES

Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO

shake the sample for

approximately 2 minutes after adding the powder reagent. If the water used for this procedure has an alkalinity value greater than 250 mg/L CaCO value greater than 150 mg/L CaCO

, the color of the sample may develop only partially, or may

rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

or acidity

Page 55

8.10. COPPER LOW RANGE

SPECIFICATIONS

Range 0.000 to 1.500 mg/L (as Cu) Resolution 0.001 mg/L Accuracy ±0.010 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA method.

REQUIRED REAGENTS Code Description Quantity

HI95747‑0 Copper Low Range Reagent 1 packet

REAGENT SETS

HI95747‑01 Reagents for 100 tests HI95747‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Copper LR method using the procedure described in the Method Selection section (see page 17).

COPPER LOW RANGE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

10 mL

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• Remove the cuvette.

• Add one packet of HI95747‑0 Copper Low Range Reagent. Replace the cap and shake gently for about 15 seconds.

COPPER LOW RANGE

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of copper (Cu).

INTERFERENCES

Interference may be caused by: Silver, Cyanide. For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted between 6 and 8.

Page 57

8.11. COPPER HIGH RANGE

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cu) Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA method.

REQUIRED REAGENTS Code Description Quantity

HI93702‑0 Copper High Range Reagent 1 packet

REAGENT SETS

HI93702‑01 Reagents for 100 tests HI93702‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Copper HR method using the procedure described in the Method Selection section (see page 17).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

COPPER HIGH RANGE

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

Page 58

• Remove the cuvette.

• Add one packet of HI93702‑0 Copper High Range Reagent. Replace the cap and shake gently for about 15 seconds.

• Reinsert the cuvette into the instrument and close the lid.

COPPER HIGH RANGE

INTERFERENCES

Interference may be caused by: Silver, Cyanide. For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted between 6 and 8.

Page 59

8.12. NITRATE

NITRATE

SPECIFICATIONS

Range 0.0 to 30.0 mg/L (as NO

‑

‑ N) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±10% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the cadmium reduction method.

REQUIRED REAGENTS Code Description Quantity

HI93728‑0 Nitrate Reagent 1 packet

REAGENT SETS

HI93728‑01 Reagents for 100 tests HI93728‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Nitrate method using the procedure described in

the Method Selection section (see page 17).

• Fill the cuvette with 10 mL of sample, (up to the mark),

and replace the cap.

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

Page 60

NITRATE

• Remove the cuvette and add one packet of HI93728‑0 Nitrate Reagent.

• Replace the cap and shake vigorously up and down for exactly

10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and method of shaking could sensitively affect the measurement.

Note: The method is technique-sensitive. See procedure on page 20 Cuvette Preparation for proper mixing technique.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 4 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of nitrate-nitrogen (NO3-N).

• Press  or  to access the second level functions.

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• Press the Chem Frm key to convert the result to mg/L of nitrate (NO3¯).

•Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Ammonia and amines, as urea and primary aliphatic amines Chloride above 100 ppm Chlorine above 2 ppm Copper Iron(III) Strong oxidizing and reducing substances Sulfide must be absent

NITRATE

Page 62

8.13. NITRITE, MARINE ULTRA LOW RANGE

SPECIFICATIONS

Range 0 to 200 µg/L (as N0

‑

‑N)

Resolution 1 µg/L Accuracy ±10 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the EPA Diazotization method 354.1.

REQUIRED REAGENTS Code Description Quantity

HI764‑25 Nitrite Ultra Low Range Reagent 1 packet

REAGENT SETS

HI764‑25 Reagents for 25 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

NITRITE, MARINE ULTRA LOW RANGE

• Select the Nitrite, Marine ULR method using the procedure

described in the Method Selection section (see page 17).

• Fill the cuvette with 10 mL of unreacted sample (up to the

mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

10 mL

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Add one packet of HI764‑25 Nitrite Ultra Low Range

Reagent. Replace the cap and shake gently for about 15 seconds.

Page 63

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 15 minutes and press Read. When the timer ends the meter will perform the reading.

The instrument displays concentration in µg/L of nitrite-nitrogen (NO

-N).

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to µg/L of nitrite (NO

¯) and sodium nitrite

(NaNO2).

NITRITE, MARINE ULTRA LOW RANGE

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by the following ions: ferrous, ferric, cupric, mercurous, silver, antimonious, bismuth, auric, lead, metavanadate and chloroplatinate. Strongly reducing and oxidizing reagents. High levels of nitrate (above 100 mg/L) could yield falsely high readings due to a minute amount of reduction to nitrite that could occur at these levels.

Page 64

8.14. NITRITE LOW RANGE

SPECIFICATIONS

Range 0 to 600 µg/L (as NO

‑

‑N)

Resolution 1 µg/L Accuracy ±20 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the EPA Diazotization method 354.1.

REQUIRED REAGENTS

NITRITE LOW RANGE

Code Description Quantity

HI93707‑0 Nitrite Low Range Reagent 1 packet

REAGENT SETS

HI93707‑01 Reagents for 100 tests HI93707‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Nitrite LR method using the procedure described

in the Method Selection section (see page 17).

• Fill the cuvette up to the mark with 10 mL of unreacted

sample (up to the mark) and replace the cap.

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

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• Add one packet of HI93707‑0 Nitrite Low Range Reagent. Replace the cap and shake gently for about 15 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 15 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of nitrite-nitrogen (NO

-N).

NITRITE LOW RANGE

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to µg/L of nitrite (NO

(NaNO2).

• Press  or  to return to the measurement screen.

) and sodium nitrite

Page 66

INTERFERENCES

NITRITE LOW RANGE

Page 67

8.15. NITRITE HIGH RANGE

NITRITE HIGH RANGE

SPECIFICATIONS

Range 0 to 150 mg/L (as NO

‑

)

Resolution 1 mg/L Accuracy ±4 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the Ferrous Sulfate method.

REQUIRED REAGENTS Code Description Quantity

HI93708‑0 Nitrite High Range Reagent 1 packet

REAGENT SETS

HI93708‑01 Reagents for 100 tests HI93708‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Nitrite HR method using the procedure described

in the Method Selection section (see page 17).

• Fill the cuvette up to the mark with 10 mL of unreacted

sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

10 mL

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

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• Add one packet of HI93708‑0 Nitrite High Range Reagent.

Replace the cap and shake gently until completely dissolved.

• Reinsert the cuvette into the instrument and close the lid.

NITRITE HIGH RANGE

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 10 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of nitrite (NO

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to mg/L of nitrite-nitrogen (NO

sodium nitrite (NaNO2).

• Press  or  to return to the measurement screen.

-N) and

Page 69

8.16. OXYGEN, DISSOLVED

SPECIFICATIONS

Range 0.0 to 10.0 mg/L (as O2) Resolution 0.1 mg/L Accuracy ±0.4 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Azide modified Winkler method.

REQUIRED REAGENTS Code Description Quantity

HI93732A‑0 Dissolved Oxygen Reagent A 5 drops HI93732B‑0 Dissolved Oxygen Reagent B 5 drops HI93732C‑0 Dissolved Oxygen Reagent C 10 drops

REAGENT SET

HI93732‑01 Reagents for 100 tests HI93732‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Oxygen (dissolved) method using the procedure

described in the Method Selection section (see page 17).

OXYGEN, DISSOLVED

• Fill one 60 mL glass bottle completely with the unreacted

sample.

• Replace the cap and ensure that a small part of the sample

spills over.

• Remove the cap and add 5 drops of HI93732A‑0 and 5 drops of

HI93732B‑0.

• Add more sample, to fill the bottle completely. Replace the cap

and ensure that a part of the sample spills over.

Note: This ensures no air bubbles have been trapped inside the bottle. Trapped air bubbles could alter readings.

• Invert the bottle several times until the sample turns orange‑

yellow and a flocculating agent appears.

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• Let the sample stand for approximately 2 minutes to allow flocculating

agent to settle.

• When the upper half of the bottle is clear, add 10 drops of

HI93732C‑0 Dissolved Oxygen Reagent C.

OXYGEN, DISSOLVED

• Replace the cap and invert the bottle until the settled flocculating

agent dissolves completely. The sample is ready for measurement when it is yellow and completely clear.

• Fill the first cuvette (#1) with 10 mL of the unreacted sample

(up to the mark), and replace the cap.

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Fill second cuvette (#2) with 10 mL of the reacted sample

(up to the mark) and replace the cap.

Page 71

• Insert the cuvette into the holder and close the lid.

OXYGEN, DISSOLVED

• Press Read to start the reading. The instrument will display the results in mg/L of oxygen (O

INTERFERENCES

Interferences may be caused by reducing and oxidizing materials.

Page 72

8.17. pH

SPECIFICATIONS

Range 6.5 to 8.5 pH Resolution 0.1 pH Accuracy ±0.1 pH at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Phenol Red method.

REQUIRED REAGENTS Code Description Quantity

HI 93710‑0 pH Reagent 5 drops

REAGENT SETS

HI 93710‑01 Reagents for 100 pH tests HI 93710‑03 Reagents for 300 pH tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the pH method using the procedure described in

the Method Selection section (see page 17).

• Fill the cuvette with 10 mL of unreacted sample (up to

the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

10 mL

Page 73

• Remove the cuvette and add 5 drops of HI93710‑0

pH Reagent Indicator. Replace the cap and mix the solution.

• Reinsert the cuvette into the instrument and close the lid.

• Press the Read key to start the reading. The instrument displays the result in pH.

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8.18. PHOSPHATE, MARINE ULTRA LOW RANGE

SPECIFICATIONS

Range 0 to 200 µg/L (as P) Resolution 1 µg/L Accuracy ±5 µg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 20th edition, Ascorbic Acid method.

REQUIRED REAGENTS Code Description Quantity

HI736‑25 Phosphorus Ultra Low Range Reagent 1 packet

REAGENT SETS

HI736‑25 Reagents for 25 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

PHOSPHATE, MARINE ULTRA LOW RANGE

• Select the Phosphate Marine ULR method using the procedure described in the Method Selection

section (see page 17).

• Rinse, cap and shake the cuvette several times with

unreacted sample.

• Fill the cuvette with 10 mL of sample (up to the

mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Add one packet of HI736‑25 Phosphorus Ultra Low

Range Reagent. Replace the cap and shake gently (for about 2 minutes) until the powder is completely dissolved.

10 mL

Page 75

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of phosphorus (P).

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to µg/L of phosphate (PO

) and phosphorus

pentoxide (P2O5).

PHOSPHATE, MARINE ULTRA LOW RANGE

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Iron above 50 mg/L Silica above 50 mg/L Silicate above 10 mg/L Copper above 10 mg/L Hydrogen sulfide, arsenate, turbid sample and highly buffered samples also interfere.

Page 76

8.19. PHOSPHATE LOW RANGE

SPECIFICATIONS

Range 0.00 to 2.50 mg/L (as PO

3‑

)

Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Ascorbic Acid method.

REQUIRED REAGENTS Code Description Quantity

HI93713‑0 Phosphate Low Range Reagent 1 packet

PHOSPHATE LOW RANGE

REAGENT SETS

HI93713‑01 Reagents for 100 tests HI93713‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Phosphate LR method using the procedure

described in the Method Selection section (see page 17).

• Rinse, cap and shake the cuvette several times with

unreacted sample.

• Fill the cuvette with 10 mL of sample (up to the mark) and

replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

10 mL

• Remove the cuvette and add the content of one packet

of HI93713‑0 Phosphate Low Range Reagent. Replace the cap and shake gently (for about 2 minutes) until the powder is completely dissolved.

Page 77

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The

instrument displays concentration in mg/L of phosphate (PO

¯).

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to mg/L of phosphorus (P) and phosphorus

pentoxide (P

2O5

PHOSPHATE LOW RANGE

• Press or  to return to the measurement screen.

INTERFERENCES

Page 78

8.20. PHOSPHATE HIGH RANGE

SPECIFICATIONS

Range 0.0 to 30.0 mg/L (as PO

3‑

)

Resolution 0.1 mg/L Accuracy ±1.0 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method.

REQUIRED REAGENTS Code Description Quantity

PHOSPHATE HIGH RANGE

HI93717A‑0 Phosphate High Range Reagent A 10 drops HI93717B‑0 Phosphate High Range Reagent B 1 packet

REAGENT SETS

HI93717‑01 Reagents for 100 tests HI93717‑03 Reagents for 300 tests

For other accessories see page 87.

MEASUREMENT PROCEDURE

• Select the Phosphate HR method using the

procedure described in the Method Selection section (see page 17).

• Fill the cuvette with 10 mL of unreacted sample

(up to the mark) and replace the cap.

10 mL

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Add 10 drops of HI93717A‑0 Phosphate High Range Reagent A.

Page 79

• Add one packet of HI93717B‑0 Phosphate HR Reagent

B to the cuvette. Replace the cap and shake gently until completely dissolved.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of phosphate (PO

¯).

PHOSPHATE HIGH RANGE

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to mg/L of phosphorus (P) and phosphorus

pentoxide (P

2O5

• Press  or  to return to the measurement screen.

Page 80

9. ERROR DESCRIPTIONS

The instrument shows clear warning messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described below.

No Light: The light source is not functioning properly.

Light Leak: There is an excess amount of ambient light

ERROR DESCRIPTIONS

reaching the detector.

Inverted Cuvette: The sample and the zero cuvettes are inverted.

Light Low: The instrument cannot adjust the light level. Please check that the sample does not contain any debris.

Light High: There is too much light to perform a measurement. Please check the preparation of the zero cuvette.

Ambient temperature out of limit: The meter is too hot or too cold for an accurate measurement. Allow the meter to reach 10 °C to 40 °C (50 °F to 104 °F) before performing a measurement.

Ambient temperature changed: The temperature of the meter has changed significantly since the zero measurement has been performed. A zero measurement must be performed again.

Out of range: The measured value is outside the limits of the method.

Page 81

10. STANDARD METHODS Description Range Method

Alkalinity 0 to 500 mg/L Colorimetric

Alkalinity, Marine 0 to 300 mg/L Colorimetric

Ammonia LR 0.00 to 3.00 mg/L Nessler

Ammonia MR 0.00 to 10.00 mg/L Nessler

Ammonia HR 0.0 to 100.0 mg/L Nessler

Calcium 0 to 400 mg/L Oxalate

Calcium Marine 200 to 600 mg/L Zincon

Chlorine, Free LR 0.00 to 5.00 mg/L DPD

Chlorine, Total LR 0.00 to 5.00 mg/L DPD

Copper LR 0.000 to 1.500 mg/L Bicinchoninate

Copper HR 0.00 to 5.00 mg/L Bicinchoninate

Nitrate 0.0 to 30.0 mg/L Cadmium reduction

Nitrite, Marine ULR 0 to 200 µg/L Diazotization

Nitrite LR 0 to 600 µg/L Diazotization

Nitrite HR 0 to 150 mg/L Ferrous Sulfate

STANDARD METHODS

Oxygen, Dissolved 0.0 to 10.0 mg/L Winkler

pH 6.5 to 8.5 pH Phenol Red

Phosphate, Marine ULR 0 to 200 µg/L Ascorbic Acid

Phosphate LR 0.00 to 2.50 mg/L Ascorbic Acid

Phosphate HR 0.0 to 30.0 mg/L Amino Acid

Page 82

11. ACCESSORIES

11.1. REAGENT SETS Code Description

HI736‑25 25 phosphate marine ULR tests

HI755‑26 25 alkalinity marine tests

ACCESSORIES

HI758‑26 25 calcium marine tests

HI764‑25 25 nitrite marine ULR tests

HI775‑26 25 alkalinity fresh water tests

HI93700‑01 100 ammonia LR tests

HI93700‑03 300 ammonia LR tests

HI93701‑01 100 chlorine free tests (powder)

HI93701‑03 300 chlorine free tests (powder)

HI93701‑F 300 chlorine free tests (liquid)

HI93701‑T 300 chlorine total tests (liquid)

HI93702‑01 100 copper HR tests

HI93702‑03 300 copper HR tests

HI93707‑01 100 nitrite LR tests

HI93707‑03 300 nitrite LR tests

HI93708‑01 100 nitrite HR tests

HI93708‑03 300 nitrite HR tests

HI93710‑01 100 pH tests

HI93710‑03 300 pH tests

HI93711‑01 100 chlorine total tests (powder)

HI93711‑03 300 chlorine total tests (powder)

HI93713‑01 100 phosphate LR tests

HI93713‑03 300 phosphate LR tests

HI93715‑01 100 ammonia MR tests

HI93715‑03 300 ammonia MR tests

Page 83

Code Description

HI93717‑01 100 phosphate HR tests

HI93717‑03 300 phosphate HR tests

HI93728‑01 100 nitrate tests

HI93728‑03 300 nitrate tests

HI93732‑01 100 dissolved oxygen tests

HI93732‑03 300 dissolved oxygen tests

HI93733‑01 100 ammonia HR tests

HI93733‑03 300 ammonia HR tests

HI937521‑01 50 calcium fresh water tests

HI937521‑03 150 calcium fresh water tests

HI95747‑01 100 copper LR tests

HI95747‑03 300 copper LR tests

ACCESSORIES

Page 84

11.2 pH ELECTRODES Code Description

HI10530 Triple ceramic, double junction, low temperature glass, refillable pH

electrode with conical tip and temperature sensor

ACCESSORIES

HI10430 Triple ceramic, double junction, high temperature glass, refillable pH

electrode with temperature sensor

HI11310 Glass body, double junction, refillable pH/temperature electrode

HI11311 Glass body, double junction, refillable pH/temperature electrode with

enhanced diagnostics

HI12300 Plastic body, double junction, gel filled, non refillable pH/temperature

electrode

HI12301 Plastic body, double junction, gel filled, non refillable pH/temperature

electrode with enhanced diagnostics

HI10480 Glass body, double junction with temperature sensor for wine analysis

FC2320 Double junction, open reference, non refillable, electrolyte viscolene, PVDF

body with conical tip, pH/temperature electrode

FC2100 Double junction, open reference, non refillable, electrolyte viscolene, glass

body with conical tip, pH/temperature electrode

FC2020 Double junction, open reference, non refillable, electrolyte viscolene, PVDF

body with conical tip, pH/temperature electrode

Note: The enhanced diagnostics information are not displayed by meter.

Page 85

11.3 pH SOLUTIONS BUFFER SOLUTIONS Code Description

HI70004P pH 4.01 Buffer Sachets, 20 mL (25 pcs.)

HI70007P pH 7.01 Buffer Sachets, 20 mL (25 pcs.)

HI70010P pH 10.01 Buffer Sachets, 20 mL (25 pcs.)

HI7001L pH 1.68 Buffer Solution, 500 mL

HI7004L pH 4.01 Buffer Solution, 500 mL

HI7006L pH 6.86 Buffer Solution, 500 mL

HI7007L pH 7.01 Buffer Solution, 500 mL

HI7009L pH 9.18 Buffer Solution, 500 mL

HI7010L pH 10.01 Buffer Solution, 500 mL

HI8004L pH 4.01 Buffer Solution in FDA approved bottle, 500 mL

HI8006L pH 6.86 Buffer Solution in FDA approved bottle, 500 mL

HI8007L pH 7.01 Buffer Solution in FDA approved bottle, 500 mL

HI8009L pH 9.18 Buffer Solution in FDA approved bottle, 500 mL

HI8010L pH 10.01 Buffer Solution in FDA approved bottle, 500 mL

ACCESSORIES

ELECTRODE STORAGE SOLUTIONS Code Description

HI70300L Storage Solution, 500 mL

HI80300L Storage Solution in FDA approved bottle, 500 mL

Page 86

ELECTRODE CLEANING SOLUTIONS Code Description

HI70000P Electrode Rinse Sachets, 20 mL (25 pcs.)

HI7061L General Cleaning Solution, 500 mL

ACCESSORIES

HI7073L Protein Cleaning Solution, 500 mL

HI7074L Inorganic Cleaning Solution, 500 mL

HI7077L Oil & Fat Cleaning Solution, 500 mL

HI8061L General Cleaning Solution in FDA approved bottle, 500 mL

HI8073L Protein Cleaning Solution in FDA approved bottle, 500 mL

HI8077L Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL

ELECTRODE REFILL ELECTROLYTE SOLUTIONS Code Description

HI 7082 3.5M KCl Electrolyte, 4x30 mL, for double junction electrodes

HI 8082 3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction electrodes.

Page 87

11.4. OTHER ACCESSORIES Code Description

HI72083300 carrying case

HI731318 cloth for wiping cuvettes (4 pcs.)

HI731331 glass cuvettes (4 pcs.)

HI731335N cap for cuvette (4 pcs.)

HI731340 200 µL automatic pipette

HI731341 1000 µL automatic pipette

HI731342 2000 µL automatic pipette

HI740034P cap for 100 mL beaker (10 pcs.)

HI740036P 100 mL plastic beaker (10 pcs.)

HI740038 60 mL glass bottle and stopper

HI740142P 1 mL graduated syringe (10 pcs)

HI740143 1 mL graduated syringe (6 pcs.)

HI740144 pipette tip (6 pcs.)

HI740157P plastic refilling pipette (20 pcs.)

HI740220 25 mL graduated glass vial (2 pcs.)

HI740223 170 mL plastic beaker

HI740224 170 mL plastic beaker (12 pcs.)

HI740225 60 mL graduated syringe

HI740226 5 mL graduated syringe

HI740227 filter assembly

HI740228 filter discs (25 pcs.)

HI 740229 100 mL graduated cylinder

DEMI‑02 demineralizer for 2 L water

HI75110/220E USB power adapter, European plug

HI75110/220U USB power adapter, USA plug

HI76404A electrode holder

Page 88

Code Description

HI83303‑11 CAL Check cuvette kit for HI83303

HI83300‑100 Sample preparation kit consisting of activated carbon for 50 tests, demineralizer bottle for 10 L of water, 100 mL graduated beaker with cap, 170 mL graduated beaker with cap, 3 mL pipette, 60 mL syringe,

ACCESSORIES

5 mL syringe, graduated cylinder, spoon, funnel, filter paper (25 pcs.).

HI920015 USB to micro USB cable connector

HI93703‑50 cuvette cleaning solution (230 mL)

HI93703‑55 activated carbon (50 pcs.)

Page 89

12. ABBREVIATIONS

EPA: US Environmental Protection Agency °C: degree Celsius °F: degree Fahrenheit µg/L: micrograms per liter (ppb) mg/L: milligrams per liter (ppm) g/L: grams per liter (ppt) mL: milliliter GLP good laboratory practice UHR ultra high range ULR ultra low range HR: high range MR: medium range LR: low range PAN: 1‑(2‑pyridylazo)‑2‑naphtol TPTZ: 2,4,6‑tri‑(2‑pyridyl)‑1,3,5‑triazine

ABBREVIATIONS

Page 90

Recommendations for Users

Before using this product make sure that they are entirely suitable for your specific application and for the environment in which they are used. Operation of these instruments may cause unacceptable interferences to other electronic equipment. Take all necessary steps to correct such interferences. Any variation introduced by the user to the supplied equipment may degrade the instruments EMC performance. To avoid damages or burns, do not put the instrument in a microwave oven. For yours and the instrument safety do not use or store the instrument in hazardous environments.

Page 91

Warranty

The HI83303 is warranted for two years against defects in workmanship and materials when used for their intended purpose and maintained according to instructions. Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered.

If service is required, contact your local Hanna Instruments Office. If under warranty, report the model number, date of purchase, serial number and the nature of the problem. If the repair is not covered by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization (RGA) number from the Technical Service department and then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for complete protection.

Hanna Instruments reserves the right to modify the design, construction or appearance of its products

without advance notice.

Page 92

World Headquarters

Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com

Local Office

Hanna Instruments USA 270 George Washington Highway Smithfield, RI 02917 Phone: 800.426.6287 Fax: 401.765.7575 e‑mail: tech@hannainst.com

MAN83303 10/16‑1

Printed in ROMANIA

Hanna Instruments HI83303 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual