HANNA HI83300-02 User guide

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HI83300

Multiparameter Photometer

for Water & Wastewater

INSTRUCTION MANUAL

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Dear Customer,

Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using this instrument. This manual will provide you with the necessary information for correct use of this instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e‑mail us at

[email protected] or view our worldwide contact list at www.hannainst.com.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

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TABLE OF CONTENTS

1. PRELIMINARY EXAMINATION ............................................................................................... 7

2. SAFETY MEASURES ............................................................................................................. 7

3. SPECIFICATIONS ................................................................................................................. 8

4. ABBREVIATIONS ................................................................................................................ 9

5. DESCRIPTION ................................................................................................................... 10

5.1. GENERAL DESCRIPTION & INTENDED USE ................................................................ 10

5.2. PRECISION & ACCURACY .......................................................................................... 10

5.3. FUNCTIONAL DESCRIPTION ...................................................................................... 11

5.4. PRINCIPLE OF OPERATION ....................................................................................... 12

5.5. OPTICAL SYSTEM ..................................................................................................... 13

6. GENERAL OPERATIONS ...................................................................................................... 14

6.1. POWER CONNECTION & BATTERY MANAGEMENT ....................................................... 14

6.2. MODE SELECTION ................................................................................................... 14

6.3. GENERAL SETUP ..................................................................................................... 15

6.4. CONTEXTUAL HELP .................................................................................................. 18

7. LOGGING DATA & DATA MANAGEMENT................................................................................ 19

7.1. LOGGING DATA ....................................................................................................... 19

7.2. ADDING SAMPLE & USER NAMES TO LOG DATA ......................................................... 19

7.3. DATA MANAGEMENT ................................................................................................ 20

8. PHOTOMETER MODE ......................................................................................................... 21

8.1. METHOD SELECTION ............................................................................................... 21

8.2. COLLECTING & MEASURING SAMPLES AND REAGENTS ................................................ 21

8.3. CUVETTE PREPARATION ........................................................................................... 22

8.4. TIMERS & MEASUREMENT FUNCTIONS ..................................................................... 24

8.5. CHEMICAL FORMULA & UNIT CONVERSION ............................................................... 24

8.6. METER VALIDATION & CAL CHECK ............................................................................. 25

8.7. ABSORBANCE MEASUREMENTS ................................................................................ 26

9. PROBE MODE ................................................................................................................... 27

9.1. pH MEASUREMENT ................................................................................................. 27

9.2. pH CALIBRATION ..................................................................................................... 28

TABLE OF CONTENTS

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9.3. pH MESSAGES & WARNINGS ................................................................................... 29

9.4. pH GLP .................................................................................................................. 30

9.5. pH ELECTRODE CONDITIONING & MAINTENANCE ....................................................... 31

10. METHOD PROCEDURES ................................................................................................... 33

10.1. ALKALINITY ........................................................................................................... 33

10.2. ALKALINITY, MARINE ............................................................................................. 35

10.3. ALUMINUM .......................................................................................................... 37

TABLE OF CONTENTS

10.4. AMMONIA LOW RANGE .......................................................................................... 41

10.5. AMMONIA MEDIUM RANGE ................................................................................... 44

10.6. AMMONIA HIGH RANGE ........................................................................................ 47

10.7. BROMINE ............................................................................................................. 50

10.8. CALCIUM .............................................................................................................. 52

10.9. CALCIUM, MARINE ................................................................................................ 55

10.10. CHLORIDE .......................................................................................................... 57

10.11. CHLORINE DIOXIDE ............................................................................................. 60

10.12. CHLORINE DIOXIDE, RAPID METHOD .................................................................... 64

10.13. CHLORINE, FREE ................................................................................................. 68

10.14. CHLORINE, FREE ULTRA LOW RANGE .................................................................... 71

10.15. CHLORINE, TOTAL ............................................................................................... 73

10.16. CHLORINE, TOTAL ULTRA LOW RANGE ................................................................... 77

10.17. CHLORINE, TOTAL ULTRA HIGH RANGE .................................................................. 79

10.18. CHROMIUM(VI) LOW RANGE ................................................................................ 81

10.19. CHROMIUM(VI) HIGH RANGE ............................................................................... 83

10.20. COLOR OF WATER ............................................................................................... 85

10.21. COPPER LOW RANGE ........................................................................................... 87

10.22. COPPER HIGH RANGE .......................................................................................... 89

10.23. CYANURIC ACID................................................................................................... 91

10.24. FLUORIDE LOW RANGE ........................................................................................ 93

10.25. FLUORIDE HIGH RANGE ....................................................................................... 95

10.26. HARDNESS, CALCIUM .......................................................................................... 98

10.27. HARDNESS, MAGNESIUM .................................................................................. 101

10.28. HARDNESS, TOTAL LOW RANGE .......................................................................... 104

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10.29. HARDNESS, TOTAL MEDIUM RANGE ................................................................... 107

10.30. HARDNESS, TOTAL HIGH RANGE ......................................................................... 110

10.31. HYDRAZINE ...................................................................................................... 113

10.32. IODINE ............................................................................................................ 115

10.33. IRON LOW RANGE ............................................................................................. 117

10.34. IRON HIGH RANGE ............................................................................................ 120

10.35. IRON(II) ........................................................................................................... 122

10.36. IRON(II)/(III) ................................................................................................... 125

10.37. MAGNESIUM .................................................................................................... 129

10.38. MANGANESE LOW RANGE .................................................................................. 131

10.39. MANGANESE HIGH RANGE ................................................................................. 134

10.40. MOLYBDENUM ................................................................................................. 137

10.41. NICKEL LOW RANGE .......................................................................................... 140

10.42. NICKEL HIGH RANGE ......................................................................................... 143

10.43. NITRATE ........................................................................................................... 145

10.44. NITRITE, MARINE ULTRA LOW RANGE ................................................................. 148

10.45. NITRITE LOW RANGE ......................................................................................... 150

10.46. NITRITE HIGH RANGE ........................................................................................ 152

10.47. OXYGEN, DISSOLVED ........................................................................................ 154

10.48. OXYGEN SCAVENGERS (CARBOHYDRAZIDE) ......................................................... 157

10.49. OXYGEN SCAVENGERS (DIETHYLHYDROXYLAMINE) (DEHA) ................................... 160

10.50. OXYGEN SCAVENGERS (HYDROQUINONE) ........................................................... 163

10.51. OXYGEN SCAVENGERS (ISO-ASCORBIC ACID) ....................................................... 166

10.52. OZONE ............................................................................................................. 169

10.53. pH ................................................................................................................... 173

10.54. PHOSPHATE, MARINE ULTRA LOW RANGE ........................................................... 175

10.55. PHOSPHATE LOW RANGE ................................................................................... 177

10.56. PHOSPHATE HIGH RANGE .................................................................................. 179

10.57. POTASSIUM ..................................................................................................... 182

10.58. SILICA LOW RANGE ........................................................................................... 185

10.59. SILICA HIGH RANGE .......................................................................................... 188

10.60. SILVER ............................................................................................................. 191

TABLE OF CONTENTS

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10.61. SULFATE .......................................................................................................... 195

10.62. SURFACTANTS, ANIONIC .................................................................................... 197

10.63. ZINC ................................................................................................................ 201

11. WARNINGS & ERRORS .................................................................................................. 203

12. STANDARD METHODS ................................................................................................... 206

13. ACCESSORIES ............................................................................................................... 209

13.1. REAGENT SETS.................................................................................................... 209

TABLE OF CONTENTS

13.2. pH ELECTRODES ................................................................................................. 213

13.3. pH SOLUTIONS ................................................................................................... 214

13.4. OTHER ACCESSORIES .......................................................................................... 216

CERTIFICATION .................................................................................................................... 218

RECOMMENDATIONS FOR USERS .......................................................................................... 218

WARRANTY ......................................................................................................................... 218

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1. PRELIMINARY EXAMINATION

Remove the instrument and accessories from the packaging and examine it carefully. For further assistance, please contact your local Hanna Instruments Office or email us at [email protected]. Each HI83300 is delivered in a rugged carrying case and is supplied with:

• Sample cuvette (4 pcs.)

• Sample cuvette cap (4 pcs.)

• Cloth for wiping cuvettes

• Scissors

• USB cable

• 5 Vdc power adapter

• 60 mL glass bottle

• Instrument quality certificate

• Instruction manual

Note: Save all packing material until you are sure that the instrument works correctly. Any damaged or defective item must be returned in its original packing material with the supplied accessories.

PRELIMINARY EXAMINATION

2. SAFETY MEASURES

• The chemicals contained in the reagent kits may be hazardous if improperly handled.

• Read the Safety Data Sheets (SDS) before performing tests.

• Safety equipment: Wear suitable eye protection and clothing when required and follow

instructions carefully.

• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of

water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released vapors.

• Waste disposal: For proper disposal of reagent kits and reacted samples, contact a

licensed waste disposal provider.

SAFETY MEASURES

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3. SPECIFICATIONS

SPECIFICATIONS

Measurement Channels

Range 0.000 to 4.000 Abs Resolution 0.001 Abs Accuracy ±0.003 Abs @ 1.000 Abs Light source Light Emitting Diode

Photometer

Probe

Temperature

Additional Specifications

Bandpass filter bandwidth 8 nm Bandpass filter wavelength accuracy ±1.0 nm Light detector Silicon photocell Cuvette types Round, 24.6 mm diameter Number of methods 68 Range -2.00 to 16.00 pH (± 1000.0 mV)* Resolution 0.01 pH (0.1 mV) Accuracy ±0.01 pH (±0.2 mV) @ 25 °C / 77 °F Temperature compensation ATC, -5.0 to 100.0 °C (23.0 to 212.0 °F)*

Calibration

Electrode Intelligent pH / temperature electrode Range -20.0 to 120.0 °C (-4.0 to 248.0 °F) Resolution 0.1 °C (0.1 °F) Accuracy ±0.5 °C @ 25 °C (±0.9 °F @ 77 °F) Logging 1000 readings (mixed photometer and electrode) Display 128 x 64 pixel B/W LCD with backlight USB-A (Host) functions Mass-storage host USB-B (Device) functions Power input, mass-storage device

Battery life

Power supply

Environment

Dimensions 206 x 177 x 97 mm (8.1 x 7.0 x 3.8") Weight 1.0 kg (2.2 lbs.)

*Limits will be reduced to actual probe / sensor limits.

5 x optical channels 1 x digital electrode channel (pH measurement)

two-point, from five available buffers (4.01, 6.86, 7.01, 9.18, 10.01 pH)

> 500 photometer measurements or 50 hours of continuous pH measurement 5 Vdc USB 2.0 power adapter / type micro-B connector

3.7 Vdc Li-polymer rechargeable battery, non-serviceable 0 to 50 °C (32 to 122 °F) 0 to 95% RH, non-serviceable

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4. ABBREVIATIONS

Abs Absorbance GLP Good Laboratory Practice NIST National Institute of Standards and Technology EPA US Environmental Protection Agency g/L grams per liter (parts per thousand, ppt) µg/L micrograms per liter (parts per billion, ppb) mg/L milligrams per liter (parts per million, ppm) HR High Range LR Low Range MR Medium Range UHR Ultra High Range ULR Ultra Low Range

ABBREVIATIONS

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5. DESCRIPTION

5.1. GENERAL DESCRIPTION & INTENDED USE

HI83300 multiparameter photometer is a compact and versatile meter with two measurement modes,

Photometer and Probe. Photometer mode includes a CAL Check™ feature and 68 different methods

DESCRIPTION

that cover a wide variety of applications, making it ideal for both benchtop and portable operations. With the CAL Check™ feature users are able to validate the performance of the instrument and apply a user calibration (if necessary). Hanna Instruments CAL Check™ cuvettes are made with NIST traceable standards. Probe mode uses a digital pH probe with a one or two-point calibration.

• Digital electrode input for pH measurements

• Certified CAL Check cuvettes to confirm meter functionality

• Dual purpose micro-USB flash drive

• Lithium polymer rechargeable battery

• Auto-off

• Absorbance mode

• User and sample name entry

• GLP features

5.2. PRECISION & ACCURACY

Precision is how closely repeated measurements are to one another. Precision is usually expressed as standard deviation. Accuracy is defined as the closeness of a test result to the true value. Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these definitions. For each method, the accuracy is expressed in the related measurement section.

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5.3. FUNCTIONAL DESCRIPTION

DESCRIPTION

1. Splash-proof keypad

2. Liquid Crystal Display (LCD)

3. Indexing mark

4. Protective port covers

5. Light-blocking cover panel

6. Cuvette holder

7. ON/OFF power button

8. 3.5 mm TRRS (jack) input for digital electrodes

9. Standard USB host connector for data transfer to a USB flash drive

10. Micro-USB device connector for power or PC interface

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Keypad Description

The keypad contains 12 direct keys and 3 functional keys with the following functions:

Press the functional key to perform the function displayed above it on the LCD.

DESCRIPTION

Press to access the list of photometer methods.

Press to move up in a menu or a help screen, to increment a set value or to access second level functions.

Press to toggle between photometer and probe (pH electrode) mode.

Press to move left in a menu or to decrement a set value.

Press to move down in a menu or a help screen, to decrement a set value or to access second level functions.

Press to move right in a menu or to increment a set value.

Press to access the setup screen.

Press to log the current reading.

Press to review saved logs.

Press to exit the current screen.

Press to display the help screen.

ON/OFF power button

5.4. PRINCIPLE OF OPERATION

Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. Photometric chemical analysis is based on specific chemical reactions between a sample and reagent to produce a light-absorbing compound. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical-chemical characteristics of the substance according to the Lambert-Beer Law. If all other factors are constant, the concentration "c" can be calculated from the absorbance of the substance.

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Lambert Beer Law:

-log I/Io = el c d

A = el c d

= intensity of incident light beam

I = intensity of light beam after absorption e

= molar extinction coefficient at wavelength l

c = molar concentration of the substance d = optical path through the substance

5.5. OPTICAL SYSTEM

DESCRIPTION

Instrument Block Diagram

The internal reference system (reference detector) of the HI83300 photometer compensates for any drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero) measurement and sample measurement. LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher luminous efficiency, providing more light while using less power. They also produce little heat, which could otherwise affect electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps have poor blue / violet light output. Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to be received. The end result is higher measurement stability and less wavelength error. A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette imperfections and scratches, eliminating the need to index the cuvette.

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GENERAL OPERATIONS

6. GENERAL OPERATIONS

6.1. POWER CONNECTION & BATTERY MANAGEMENT

The meter can be powered from an AC / DC adapter (included) or from the built-in rechargeable battery. The meter will perform an auto-diagnostic test when it is first powered on. During this test, the Hanna Instruments logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display. The battery icon on the LCD will indicate the battery status:

• battery is charging from external adapter • battery fully charged (meter connected to AC / DC adapter)

• battery capacity (no external adapter) • battery near 0% (no external adapter)

• battery exhausted (no external adapter)

To conserve battery, the meter will turn off automatically after 15 minutes of inactivity (30 minutes after a Zero measurement). If a photometer measurement is on the screen, an auto-log is created before shutdown.

6.2. MODE SELECTION

The HI83300 has two operational modes: Photometer and Probe. Photometer mode enables on-demand measurement of a cuvette using the integrated optical system. Probe mode enables continuous measurement using a Hanna digital electrode connected to the

3.5 mm port. To switch between Photometer mode and Probe mode, use the MODE key.

Note: The active mode cannot be switched while in Setup, Recall or Method menus.

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6.3. GENERAL SETUP

Press the SETUP key to enter in Setup menu, highlight desired option using the  keys and press Select.

CAL Check (Photometer Mode Only)

Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are displayed on the screen. To start a new CAL Check press Check and follow the prompts on the screen. See METER VALIDATION & CAL CHECK for additional information.

Temperature Unit (Probe Mode Only)

Option: °C or °F

Press the functional key to select the desired temperature unit.

Backlight

Values: 0 to 8

Press Modify to access the backlight intensity. Use the functional keys or the  keys to increase or decrease the value. Press Accept to confirm or press the ESC key to return to the Setup menu without saving the new value.

GENERAL OPERATIONS

Contrast

Values: 0 to 20

Press Modify to change the display’s contrast. Use the functional keys or the  keys to increase or decrease the value. Press Accept to confirm the value or the ESC key to return to the Setup menu without saving the new value.

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Date & Time

Press Modify to change the date and time. Press the functional keys or the  keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the  keys to change the value. Press Accept to confirm or ESC key to return to the Setup without saving the new date or time.

GENERAL OPERATIONS

Time Format

Option: AM/PM or 24-hour

Press the functional key to select the desired time format.

Date Format

Option: DD/MM/YYYY, MM/DD/YYYY, YYYY/MM/DD, YYYY-MM-DD, Mon DD, YYYY, DD-Mon-YYYY, YYYY-Mon-DD

Press Modify to change the date format. Use the  keys to select the desired format. Press Select to confirm or the ESC key to return to the Setup menu without saving the new format.

Decimal Separator

Option: Comma ( , ) or Period ( . )

Press the functional key to select the desired decimal separator. The decimal separator is used on the measurement screen and CSV (Comma-Separated Values) files.

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Language

Option: Português, Deutsch, English, Español, Français, Italiano, Dutch

Press Modify to change the language. Use the  keys to select the desired language. Press Select to change the language.

Beeper

Option: Enable or Disable

When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error is detected. Press the functional key to enable or disable the beeper.

Instrument ID

Option: 0 to 999999

This option is used to set the instrument’s ID (identification number). Press Modify to access the instrument ID screen. Use the functional keys or the  keys to highlight the digit to be modified. Press the  keys in order to set the desired value. Press Accept to confirm the value or press the ESC key to return to the Setup menu without saving the new value.

GENERAL OPERATIONS

Meter Information

Press Select to view the model, serial number, firmware version and selected language. Press the ESC key to return to the Setup menu.

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Probe Information (pH Mode Only)

Press Select to view model number, serial number and firmware version for the connected probe. Press the ESC key to return to the Setup menu.

GENERAL OPERATIONS

6.4. CONTEXTUAL HELP

HI83300 offers an interactive contextual help mode that assists the user at any time.

To access the help screen press the HELP key. The instrument will display additional information related to the current screen. To read all the available information, scroll the text using the  keys. Press the ESC key to return to the previous screen.

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7. LOGGING DATA & DATA MANAGEMENT

The instrument features a data log function to help you keep track of all your analysis. The data log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the

LOG and RECALL keys.

7.1. LOGGING DATA

Press the LOG key and the last valid measurement will be stored with a date and time stamp. Only valid measurements can be stored.

7.2. ADDING SAMPLE & USER NAMES TO LOG DATA

A sample ID and user ID can be added to the saved log. Use the  keys to highlight the Sample ID or User ID then press Modify. Sample ID and user ID are entered using the alphanumeric multitapping keypad.

LOGGING DATA & DATA MANAGEMENT

Enter one character at a time by pressing the key with the assigned character repeatedly until the desired character is highlighted. For reference, a list of the characters available for the current key will be shown under the text box. The character will be entered after a two-second delay or after another key is pressed.

Press Accept to update the sample or user ID. Press  functional key to delete the last character. Press Clear to delete all of the characters. Press the ESC key to discard all changes and return to the previous screen.

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7.3. DATA MANAGEMENT Viewing & Deleting

Data can be viewed, deleted and exported to a USB drive or a PC by pressing the RECALL key. Use the  keys to scroll through the saved logs. Press Info to view additional information about the selected log.

Use Delete to erase logged data. After pressing Delete the prompt on the display will confirm the action.

LOGGING DATA & DATA MANAGEMENT

Press No or the ESC key to return to the previous screen. Press Yes to delete the selected log. Press Del All to erase all the logged data. If Del All is pressed the prompt on the display will confirm the action. Press Yes to delete all logged data, No or the ESC key to return to the log recall.

Data Export

Log data can be exported to a USB flash drive or to a PC. To access data export functions, press the RECALL key then Export.

Use the  keys to select the desired export location. For export to flash drive, insert the USB flash drive into the dedicated port at the back of the meter labeled HOST USB, then follow the on-screen prompts. For export to PC, connect the meter to a PC using the supplied micro-USB cable. Insert the cable into the port at the back of the meter labeled PC PWR. Follow the on-screen prompts. When the meter says PC connected, the meter will appear as a removable disk. Use a file manager (such as Windows Explorer or Mac Finder) to move the file from the meter to the PC. Log data is exported as a single file (HI83300.csv) containing all logged photometer and probe data. The CSV file may be opened with a text editor or spreadsheet application.

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8. PHOTOMETER MODE

8.1. METHOD SELECTION

In order to select the desired method press the METHOD key and a screen with the available methods will appear. Press the  keys to highlight the desired method. Press Select.

After the desired method is selected, follow the procedure described in the related section. Before performing a method, read all the instructions carefully.

8.2. COLLECTING & MEASURING SAMPLES AND REAGENTS Proper Use of Syringe

1. Push the plunger completely into the syringe and insert the tip into the solution.

2. Pull the plunger up until the lower edge of the seal is exactly on the mark for the desired volume.

3. Take out the syringe and clean the outside of the syringe tip, be sure that no drops are hanging on the tip of the syringe. Then, keeping the syringe in a vertical position, push the plunger down into the syringe, the desired volume has been delivered.

PHOTOMETER MODE

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Proper Use of Dropper Bottle

1. Tap the dropper on the table several times.

2. Remove the cap and wipe the outside of the tip with a cloth.

3. Keep the dropper bottle in a vertical position while dosing the reagent.

PHOTOMETER MODE

Proper Use of Powder Packet

1. Use scissors to open the powder packet.

2. Push the edges of the packet to form a spout.

3. Pour out the content of the packet.

8.3. CUVETTE PREPARATION

Proper mixing is very important for reproducibility of the measurements. The proper mixing technique for each method is listed in the method procedure.

(a) Invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical

position. Turn the cuvette upside-down and wait for all of the solution to flow to the cap end, then return the cuvette to the upright vertical position and wait for all of the solution to flow to the cuvette bottom. This is one inversion. The correct speed for this mixing technique is 10 to 15 complete inversions in 30 seconds. This mixing technique is indicated with “invert to mix” and one of the following icons:

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(b) Shaking the cuvette, moving the cuvette up and down. The movement may be gentle or vigorous.

This mixing technique is indicated with “shake gently” or “shake vigorously”, and one of the following icons:

shake gently shake vigorously

following icons:

In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied High-Density Polyethylene (HDPE) plastic stopper and then the black cap.

PHOTOMETER MODE

Whenever the cuvette is placed into the measurement holder, it must be dry outside and free of fingerprints, oil and dirt. Wipe it thoroughly with

HI731318 microfiber cleaning cloth or a lint-free wipe prior to insertion.

Shaking the cuvette can generate bubbles in the sample, causing higher readings. To obtain accurate measurements, remove such bubbles by swirling or by gently tapping the cuvette.

Do not let the reacted sample stand too long after reagent is added. For best accuracy, respect the timings described in each specific method. It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for each sample and to use the same cuvette for zeroing and measurement when possible.

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Discard the sample immediately after the reading is taken, or the glass might become permanently stained. All the reaction times reported in this manual are at 25 °C (77 °F). In general, the reaction time should be increased for temperatures lower than 20 °C (68 °F) and decreased for temperatures higher than 25 °C (77 °F).

Interferences

In the method measurement section the most common interferences that may be present in a typical

PHOTOMETER MODE

water sample have been reported. It is possible that a particular application could introduce other compounds that will also interfere.

8.4. TIMERS & MEASUREMENT FUNCTIONS

Each method requires a different preparation procedure, reaction times and sample preparations. If a timer or timers are necessary for proper sample preparation, the Timer will be available. To use a reaction timer, press Timer. The default timer will start immediately. To stop and reset the timer, press Stop. If the selected method requires more than one timer, the meter will automatically select each timer in the appropriate order. To bypass the default order, you may press the desired key to activate a different timer (only while the current timer is stopped). Press Continue to start the active timer. For some methods, the timer is only necessary after a Zero measurement has been performed. In this case, the timer key will only be available after the Zero measurement has been performed. If the method requires a Zero or Read measurement after a timer has expired, the meter will automatically perform the appropriate action. Follow the instructions in the method procedure. To perform a Zero or Read measurement, insert the prepared cuvette, then press Zero or Read. A Zero measurement must be conducted before a Read measurement.

8.5. CHEMICAL FORMULA & UNIT CONVERSION

Chemical formula and unit conversion factors are pre-programmed into the instrument and are method specific. In order to view the displayed result in the desired chemical formula press the  keys to access the second level function and then press Chem Frm to toggle between the available chemical formulas for the selected method.

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8.6. METER VALIDATION & CAL CHECK

Warning: Do not validate the meter with standard solutions other than the Hanna Instruments

CAL Check Standards. For accurate validation results, please perform tests at room temperature, 18 to 25 °C (64.4 to 77.0 °F).

Validation of the HI83300 involves absorbance measurements of certified Hanna Instruments

CAL Check Standards (see ACCESSORIES section). The CAL Check screen guides the user through the measurement of each CAL Check Standard and applies the factory calibration corrections to each measurement. The HI83300 stores the results of the most recent CAL Check measurements which may be viewed on the CAL Check screen. Compare these results with the values printed on the Certificate provided with each Hanna Instruments® CAL Check Standards kit. To perform a validation:

1. Press the SETUP key.

2. Highlight CAL Check, then press Select.

PHOTOMETER MODE

3. Follow the prompts on the screen. The meter will prompt to measure each cuvette provided in the Hanna Instruments® CAL Check Standards kit. To exit the process at any time, press ESC key.

4. Press the ESC key to return in Setup menu.

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8.7. ABSORBANCE MEASUREMENTS

Raw absorbance measurements may be performed on the HI83300 for personal or diagnostic purposes. For example, you may monitor the stability of a reagent blank by occasionally measuring its absorbance versus deionized water. To measure the raw absorbance of a prepared sample:

1. Press the METHOD key.

PHOTOMETER MODE

2. Highlight the appropriate absorbance method (according to the wavelength to be used), then

press Select. Absorbance methods are located at the bottom of the method list.

3. Prepare the sample cuvette according to the method.

4. Insert a cuvette filled with deionized water, then press Zero.

5. Insert the prepared sample cuvette, then press Read.

Warning: Never use absorbance methods for validation using Hanna Instruments cuvettes. The factory calibration corrections for CAL Check cuvettes are applied while in CAL Check mode only!

CAL Check

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9. PROBE MODE

9.1. pH MEASUREMENT

The HI83300 can be used to perform direct pH measurements by connecting a Hanna Instruments digital pH electrode with a 3.5 mm TRRS connector. Connect the electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter. If the meter is in Photometer mode, set the meter to Probe mode by pressing the MODE key.

Press Calibrate to open the calibration window. Press GLP to review the calibration information. Press Range to switch between pH and mV. For high accuracy it is recommended to calibrate your electrode often. pH electrodes should be recalibrated at least once per week, but daily calibration is recommended. Always recalibrate after cleaning an electrode, see pH CALIBRATION section for more information. To take pH measurements:

• Remove the protective cap and rinse the electrode with water.

• Collect some sample in a clean, dry beaker.

• Preferably, rinse the electrode with a small amount of sample.

• Submerse the electrode tip approximately 3 cm (1¼”) into the sample to be tested and stir the sample gently. Make sure the electrode junction is completely submersed.

• Allow time for the electrode to stabilize in the sample. When the symbol disappears, your reading is stable.

If measurements are taken successively in different samples, it is recommended to rinse the electrodes thoroughly with deionized or distilled water and then with some of the next sample to prevent cross-contamination. pH measurements are affected by temperature. Hanna Instruments® digital pH electrodes include a built-in temperature sensor and automatically calculate corrected pH values. The measured temperature is displayed on the screen with the pH measurements.

PROBE MODE

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9.2. pH CALIBRATION

From the probe measurement screen, press Calibrate to begin the calibration process. During pH calibration, the display will show the current pH reading, temperature reading, selected buffer type and the buffer number (“Buffer: 1” for the first buffer, “Buffer: 2” for the second buffer).

PROBE MODE

Press Clear to clear the current calibration. Press Confirm to accept the current calibration point (only available if the reading is stable and within the limits for the selected buffer). Press the  keys to cycle through the list of available buffers: pH 4.01, 6.86, 7.01, 9.18, 10.01. Press the ESC key to exit calibration and return to pH measurement mode.

Preparation

Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences. For accurate calibrations and to minimize cross-contamination, use two beakers for each buffer solution: one for rinsing the electrode and one for calibration. If you are measuring in the acidic range, use pH 7.01 or 6.86 as the first buffer and pH 4.01 as the second buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as the first buffer and pH

10.01 or 9.18 as the second buffer.

Procedure

Calibration can be performed using one or two calibration buffers. For more accurate measurements, a two-point calibration is recommended. Submerse the pH electrode approximately 3 cm (1¼”) into a buffer solution and stir gently. When the reading is stable and close to the selected buffer, press Confirm to accept and store the calibration point. The meter will prompt for the second buffer (Buffer: 2). To use only a one-point calibration, press the ESC key to exit calibration mode. The meter will store the calibration information to the probe and return to measurement mode. To continue calibrating with a second buffer, rinse and submerse the pH electrode approximately 3 cm (1¼”) into the second buffer solution and stir gently. If necessary, use the  keys to select a different buffer value. When the reading is stable and close to the selected buffer, press Confirm to accept and store the second calibration point. The meter will store the two-point calibration information to the probe and return to Measurement mode. The list of calibrated buffers will appear at the bottom of the screen.

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9.3. pH MESSAGES & WARNINGS

No Probe

No probe is connected or the probe is broken.

Connecting

The meter has detected a probe and is reading the probe configuration and calibration information.

Incompatible Probe

The connected probe is not compatible with this device.

Incompatible Calibration

The probe’s current calibration is not compatible with this meter. The calibration must be cleared to use this probe.

Exceeded Probe Range

The pH and / or temperature measurement exceed the specifications of the probe. The measurement value(s) will be blinking.

PROBE MODE

Broken Temperature Sensor

The temperature sensor inside the probe is broken. Temperature compensation will revert to a fixed value of 25 °C (77 °C).

Cal Due

The probe has no calibration. See pH CALIBRATION section for details.

Clean Probe

The offset is outside the accepted window or the slope is under the accepted lower limit. Cleaning the probe will improve the pH electrode's response, repeat the calibration after cleaning. See pH ELECTRODE CONDITIONING & MAINTENANCE section for details.

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Check Probe & Buffer

There is a large difference between the pH measurement and the selected buffer value or the electrode slope is outside of the accepted slope limit. Clean the probe and confirm the correct buffer selection.

PROBE MODE

Wrong Temperature

The buffer temperature is outside of the acceptable window for the selected buffer value.

9.4. pH GLP

Good Laboratory Practice (GLP) refers to a quality control function used to ensure uniformity and consistency of sensor calibrations and measurements. To view the GLP information, press the GLP key from the probe measurement screen.

The pH GLP screen displays the date and time, buffers, slope and offset for the last calibration. If the probe has not been calibrated, “No User Calibration” is displayed. Press the ESC key to return to the measurement mode.

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9.5. pH ELECTRODE CONDITIONING & MAINTENANCE

PROBE MODE

Remove the protective cap. Do not be alarmed if salt deposits are present, this is normal. Rinse the probe with water. Shake the electrode down as you would do with a clinical thermometer to eliminate any air bubbles inside the glass bulb. If the bulb and / or junction are dry, soak the electrode in HI70300 or HI80300 Storage solution for a minimum of 30 minutes. Rinse with water. Calibrate before using. For refillable electrodes if the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI7082 or HI8082 3.5M KCl Electrolyte solution. Unscrew the fill hole cover during measurements so the liquid reference junction maintains an outward flow of electrolyte.

Storage Procedure

To minimize clogging and ensure a quick response time, the glass bulb and the junction should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of HI70300 or HI80300 Storage solution or Filling solution (HI7082 or HI8082 3.5M KCl Electrolyte solution). pH 4.01 or 7.01 buffer can also be used.

Note: Never store the electrode in distilled or deionized water.

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Periodic Maintenance

Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable, connectors must be perfectly clean and dry. If there are any scratches or cracks on the electrode stem or bulb, replace the electrode. For refillable electrodes, refill the reference chamber with fresh electrolyte (HI7082 or HI8082 3.5M

PROBE MODE

KCl Electrolyte solution). Allow the electrode to stand upright for 1 hour.

Cleaning Procedure

Several cleaning solutions are available:

• General – Soak in Hanna HI7061 or HI8061 General cleaning solution for approximately 30 minutes.

• Protein –Soak in Hanna HI7073 or HI8073 Protein cleaning solution for 15 minutes.

• Inorganic – Soak in Hanna HI7074 Inorganic cleaning solution for 15 minutes.

• Oil and grease – Rinse with Hanna HI7077 or HI8077 Oil and Fat cleaning solution.

After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte (refillable electrodes only) and soak the electrode in

HI70300 or HI80300 Storage solution for at least 1 hour before taking measurements.

Temperature Correlation for pH Sensitive Glass

Verify the temperature range by reading the limits on electrode’s cap. The pH electrode’s life is temperature dependent. If constantly cycled between two temperatures, the life of the electrode is drastically reduced.

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10. METHOD PROCEDURES

10.1. ALKALINITY

SPECIFICATIONS

Range 0 to 500 mg/L (as CaCO Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method

REQUIRED REAGENTS Code Description Quantity

HI775S Alkalinity Reagent 1 mL HI93755-53 Chlorine Removal Reagent 1 drop

REAGENT SETS

HI775-26 Reagents for 25 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Alkalinity method using the procedure described in the METHOD SELECTION section.

)

METHOD PROCEDURES

ALKALINITY

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

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• Remove the cuvette.

ALKALINITY

METHOD PROCEDURES

• Add 1mL of HI775S Alkalinity Reagent to the sample using a 1 mL syringe.

• Replace the plastic stopper and the cap. Invert 5 times.

• Insert the cuvette into the holder and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L calcium carbonate

(CaCO

1 mL

INTERFERENCES

Interference may be caused by:

• Chlorine must be absent, to remove the interference add one drop of HI93755-53 Chlorine Removal Reagent to the unreacted sample

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10.2. ALKALINITY, MARINE

SPECIFICATIONS

Range 0 to 300 mg/L (as CaCO Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method

REQUIRED REAGENTS Code Description Quantity

HI755S Alkalinity Reagent 1 mL

REAGENT SETS

HI755-26 Reagents for 25 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Alkalinity Marine method using the procedure described in the METHOD SELECTION section.

)

METHOD PROCEDURES

ALKALINITY, MARINE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

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• Remove the cuvette.

1 mL

ALKALINITY, MARINE

• Add 1 mL of HI755S Alkalinity Reagent to the sample using a 1mL syringe.

METHOD PROCEDURES

• Replace the plastic stopper and the cap. Invert 5 times.

• Insert the cuvette into the holder and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L as calcium carbonate

(CaCO

• Press the  or  key to access the second level functions.

• Press Chem Frm to convert the result to degree KH (dKH).

• Press the  or  key to return to the measurement screen.

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10.3. ALUMINUM

METHOD PROCEDURES

ALUMINUM

SPECIFICATIONS

Range 0.00 to 1.00 mg/L (as Al Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Aluminon Method

REQUIRED REAGENTS Code Description Quantity

HI93712A-0 Aluminum Reagent A 1 packet HI93712B-0 Aluminum Reagent B 1 packet HI93712C-0 Aluminum Reagent C 1 packet

REAGENT SETS

HI93712-01 Reagents for 100 tests HI93712-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Aluminum method using the procedure described in the METHOD SELECTION section.

• Fill a graduated beaker with 50 mL of sample.

)

• Add one packet of HI93712A-0 Aluminum Reagent A and mix until completely dissolved.

• Add one packet of HI93712B-0 Aluminum Reagent B and mix until completely dissolved.

Page 38

• Fill two cuvettes with 10 mL of sample (up to the mark).

ALUMINUM

• Add one packet of HI93712C-0 Aluminum Reagent C to one cuvette (#1). Replace the plastic stopper and the cap. Shake gently until completely dissolved. This is the blank.

METHOD PROCEDURES

• Insert the first cuvette (#1) into the holder and close the lid.

• Press Timer and the display will show the countdown prior to the zero or wait 15 minutes and then press Zero. The display will show “-0.0-“ when the meter is zeroed and ready for measurement.

# 1

10 mL

# 1

10 mL

# 2

• Remove the blank and insert the second cuvette (#2) into the holder and close the lid.

# 2

Page 39

• Press Read to start the reading. The instrument displays the results in mg/L of aluminum (Al3+).

• Press the  or  key to access the second level functions.

METHOD PROCEDURES

ALUMINUM

• Press Chem Frm to convert the result to mg/L of aluminum oxide (Al

• Press the  or  key to return to the measurement screen.

INTERFERENCES

Interference may be caused by:

• Alkalinity above 1000 mg/L

• Phosphate above 50 mg/L

• Iron above 20 mg/L

• Fluoride must be absent. If the fluoride concentration is known, the aluminum concentration can be determined using the graph below:

2O3

Page 40

To use the fluoride interference graph:

1. Follow the measurement procedure to obtain the aluminum concentration.

2. Locate the aluminum reading on x-axis.

3. Follow the line up, until it intersects the fluoride curve corresponding to the fluoride concentration

ALUMINUM

METHOD PROCEDURES

in the sample.

4. From the intersection of the fluoride and aluminum line, follow the line to the left until it intersects the y-axis. This point corresponds to the corrected aluminum concentration in the sample.

E.g. Aluminum reading on meter 0.40 ppm and fluoride content in sample 0.50 ppm, corrected aluminum concentration in sample is 0.75 ppm.

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10.4. AMMONIA LOW RANGE

SPECIFICATIONS

Range 0.00 to 3.00 mg/L (as NH Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426 Nessler Method

REQUIRED REAGENTS Code Description Quantity

HI93700A-0 Ammonia Low Range Reagent A 4 drops HI93700B-0 Ammonia Low Range Reagent B 4 drops

REAGENT SETS

HI93700-01 Reagents for 100 tests HI93700-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Ammonia LR method using the procedure described in the METHOD SELECTION section.

-N)

METHOD PROCEDURES

AMMONIA LOW RANGE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

Page 42

• Remove the cuvette.

• Add 4 drops of HI93700A-0 Ammonia Low Range Reagent A. Replace the plastic stopper and the cap. Swirl to mix the solution.

METHOD PROCEDURES

AMMONIA LOW RANGE

• Add 4 drops of HI93700B-0 Ammonia Low Range Reagent B. Replace the plastic stopper and the cap. Swirl to mix the solution.

• Insert the cuvette into the holder and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or wait 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH

-N).

×4

• Press the  or  key to access the second level functions.

Page 43

• Press Chem Frm to convert the result to mg/L of ammonia (NH3) and ammonium (NH

• Press the  or  key to return to the measurement screen.

INTERFERENCES

Interference may be caused by:

• Hardness above 1 g/L

• Iron

• Sulfide may cause turbidity

• Organic compounds like acetone above 0.1%, alcohols, aldehydes, aliphatic and aromatic amines, chloramines, glycine, or urea above 10 mg/L, to remove the interference distillation is required

METHOD PROCEDURES

AMMONIA LOW RANGE

Page 44

10.5. AMMONIA MEDIUM RANGE

SPECIFICATIONS

Range 0.00 to 10.00 mg/L (as NH Resolution 0.01 mg/L Accuracy ±0.05 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler Method

-N)

METHOD PROCEDURES

AMMONIA MEDIUM RANGE

REQUIRED REAGENTS Code Description Quantity

HI93715A-0 Ammonia Medium Range Reagent A 4 drops HI93715B-0 Ammonia Medium Range Reagent B 4 drops

REAGENT SETS

HI93715-01 Reagents for 100 tests HI93715-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Ammonia MR method using the procedure described in the METHOD SELECTION section.

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

10 mL

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Page 45

• Remove the cuvette.

METHOD PROCEDURES

AMMONIA MEDIUM RANGE

• Add 4 drops of HI93715A-0 Ammonia Medium Range Reagent A.

×4

Replace the plastic stopper and the cap. Swirl to mix the solution.

• Add 4 drops of HI93715B-0 Ammonia Medium Range Reagent B.

×4

Replace the plastic stopper and the cap. Swirl to mix the solution.

• Insert the cuvette into the holder and close the lid.

-N).

• Press the  or  key to access the second level functions.

Page 46

• Press Chem Frm to convert the result to mg/L of ammonia (NH3) and ammonium (NH

• Press the  or  key to return to the measurement screen.

METHOD PROCEDURES

INTERFERENCES

AMMONIA MEDIUM RANGE

Interference may be caused by:

• Hardness above 1 g/L

• Iron

• Sulfide may cause turbidity

• Organic compounds like acetone above 0.1%, alcohols, aldehydes, aliphatic and aromatic amines, chloramines, glycine, or urea above 10 mg/L, to remove the interference distillation is required

Page 47

10.6. AMMONIA HIGH RANGE

SPECIFICATIONS

Range 0.0 to 100.0 mg/L (as NH Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler Method

REQUIRED REAGENTS Code Description Quantity

HI93733A-0 Ammonia High Range Reagent A 4 drops HI93733B-0 Ammonia High Range Reagent B 9 mL

REAGENT SETS

HI93733-01 Reagents for 100 tests HI93733-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Ammonia HR method using the procedure described in the METHOD SELECTION section.

-N)

METHOD PROCEDURES

AMMONIA HIGH RANGE

• Add 1 mL of unreacted sample to the cuvette using a 1 mL syringe.

• Use the pipette to fill the cuvette up to the 10 mL mark with HI93733B-0 Ammonia High Range Reagent B. Replace the plastic stopper and the cap. Swirl to mix the solution.

• Insert the cuvette into the holder and close the lid.

10 mL

1 mL

Page 48

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

METHOD PROCEDURES AMMONIA HIGH RANGE

• Add 4 drops of HI93733A-0 Ammonia High Range Reagent A. Replace the plastic stopper and the cap. Swirl to mix the solution.

• Insert the cuvette into the holder and close the lid.

-N).

×4

• Press the  or  key to access the second level functions.

Page 49

• Press Chem Frm to convert the result to mg/L of ammonia (NH3) and ammonium (NH

• Press the  or  key to return to the measurement screen.

INTERFERENCES

Interference may be caused by:

• Hardness above 1 g/L

• Iron

• Sulfide may cause turbidity

• Organic compounds like acetone above 0.1%, alcohols, aldehydes, aliphatic and aromatic amines, chloramines, glycine, or urea above 10 mg/L, to remove the interference distillation is required

METHOD PROCEDURES

AMMONIA HIGH RANGE

Page 50

10.7. BROMINE

SPECIFICATIONS

Range 0.00 to 8.00 mg/L (as Br

BROMINE

Resolution 0.01 mg/L Accuracy ±0.08 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th Edition, DPD Method

)

METHOD PROCEDURES

REQUIRED REAGENTS Code Description Quantity

HI93716-0 Bromine Reagent 1 packet

REAGENT SETS

HI93716-01 Reagents for 100 tests HI93716-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Bromine method using the procedure described in the METHOD SELECTION section.

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

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• Remove the cuvette.

• Add one packet of HI93716-0 Bromine Reagent. Replace the plastic stopper and the cap. Shake gently for about 20 seconds to dissolve most of the reagent.

• Insert the cuvette into the holder and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or wait 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of bromine (Br

METHOD PROCEDURES

BROMINE

INTERFERENCES

Interference may be caused by:

• Chlorine, Iodine, Ozone, Oxidized forms of Chromium and Manganese

• Hardness greater than 500 mg/L CaCO3, to remove the interference shake the sample for approximately 1 minute after adding the reagent

• Alkalinity greater than 300 mg/L CaCO3 or acidity greater than 150 mg/L CaCO3, the color of the sample may develop only partially or rapidly fade, to remove the interference neutralize the sample with diluted HCl or NaOH

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10.8. CALCIUM

SPECIFICATIONS

CALCIUM

METHOD PROCEDURES

Range 0 to 400 mg/L (as Ca Resolution 1 mg/L Accuracy ±10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Oxalate Method

REQUIRED REAGENTS Code Description Quantity

– Buffer Reagent 4 drops HI93752A-Ca Calcium Reagent A 7 mL HI93752B-Ca Calcium Reagent B 1 mL

REAGENT SETS

HI937521-01 Reagents for 50 tests HI937521-03 Reagents for 150 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Calcium method using the procedure described in the METHOD SELECTION section.

)

• Add 3 mL of unreacted sample to the cuvette using the 5 mL syringe.

3 mL

• Use the pipette to fill the cuvette up to the 10 mL mark with the HI93752A-Ca Calcium Reagent A.

10 mL

×4

• Add 4 drops of Buffer Reagent.

Page 53

• Replace the plastic stopper and the cap. Invert several times to mix.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

• Add 1 mL of HI93752B-Ca Calcium Reagent B to the sample using the 1 mL syringe. Replace the plastic stopper and the cap. Invert the cuvette 10 times to mix (about 15 seconds).

1 mL

METHOD PROCEDURES

CALCIUM

• Insert the cuvette into the holder and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or wait 5 minutes.

Page 54

• After waiting 5 minutes, invert the cuvette 10 times to mix (about

CALCIUM

15 seconds).

• Insert the cuvette into the holder and close the lid.

METHOD PROCEDURES

• Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca

INTERFERENCES

Interference may be caused by:

• Acidity, Alkalinity above 1000 mg/ L CaCO

• Magnesium above 400 mg/L

Page 55

10.9. CALCIUM, MARINE

METHOD PROCEDURES

CALCIUM, MARINE

SPECIFICATIONS

Range 200 to 600 mg/L (as Ca Resolution 1 mg/L Accuracy ±6% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Zincon Method

REQUIRED REAGENTS Code Description Quantity

HI7581 Calcium Reagent A 1 mL HI7582 Calcium Reagent B 1 packet

REAGENT SETS

HI758-26 Reagents for 25 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Calcium Marine method using the procedure described in the METHOD SELECTION section.

• Add 1 mL of HI7581 Calcium Reagent A to the cuvette using a 1 mL syringe.

)

1 mL

• Use the plastic pipette to fill the cuvette to the 10 mL mark with deionized water. Replace the plastic stopper and the cap. Invert 3 to 5 times to mix.

• Insert the cuvette into the holder and close the lid.

10 mL

Page 56

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

CALCIUM, MARINE

• Remove the cuvette.

METHOD PROCEDURES

• Use the minipipette to add 0.1 mL of sample to the cuvette.

• Add one packet of HI7582 Calcium Reagent B. Replace the plastic stopper and the cap and shake vigorously for 15 seconds or until the powder is completely dissolved. Allow air bubbles to dissipate for 15 seconds before taking a reading.

0.1 mL

• Insert the cuvette into the holder and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca

Page 57

10.10. CHLORIDE

METHOD PROCEDURES

CHLORIDE

SPECIFICATIONS

Range 0.0 to 20.0 mg/L (as Cl Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±6% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Mercury(II) Thiocyanate Method

REQUIRED REAGENTS Code Description Quantity

HI93753A-0 Chloride Reagent A 1 mL HI93753B-0 Chloride Reagent B 1 mL

REAGENT SETS

HI93753-01 Reagents for 100 tests HI93753-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Chloride method using the procedure described in the METHOD SELECTION section.

• Fill one cuvette (#1) with 10 mL of deionized water (up to the mark).

)

#1 10 mL

• Fill a second cuvette (#2) with 10 mL of sample (up to the mark).

Note: For samples with low chloride ion concentration, rinse the cuvette a few times with sample before filling it with 10 mL of sample. For the most accurate results, use two graduated pipettes to deliver exactly 10 mL of deionized water and 10 mL of sample to the cuvettes.

#2 10 mL

Page 58

CHLORIDE

• Add 0.5 mL of HI93753A-0 Chloride Reagent A to each cuvette using the 1 mL syringe.

METHOD PROCEDURES

• Replace the plastic stoppers and the caps. Mix each cuvette by inverting for approximately 30 seconds.

• Add 0.5 mL of HI93753B-0 Chloride Reagent B to each cuvette using the second 1 mL syringe.

0.5 mL#20.5 mL

• Replace the plastic stoppers and the caps. Mix each cuvette by inverting for approximately 30 seconds.

• Insert the cuvette with the reacted deionized water (#1) into the holder and close the lid.

Page 59

• Press Timer and the display will show the countdown prior to the zero or wait 2 minutes and press Zero. The display will show "-0.0-" when the meter is zeroed and ready for measurement.

METHOD PROCEDURES

CHLORIDE

• Remove the cuvette.

• Insert the other cuvette (#2) with the reacted sample into the holder and close the lid.

• Press Read to start reading. The instrument displays the results in mg/L of chloride (Cl

INTERFERENCES

Interference may be caused by:

• Intensely colored samples, samples should be adequately treated before performing the test

• Suspended matter in large amount should be removed by prior filtration

• Alkaline samples, neutralize before adding reagents, the pH of the sample after addition of reagents should be about 2

Page 60

10.11. CHLORINE DIOXIDE

SPECIFICATIONS

Range 0.00 to 2.00 mg/L (as ClO Resolution 0.01 mg/L Accuracy ±0.10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the Chlorophenol Red Method

CHLORINE DIOXIDE METHOD PROCEDURES

REQUIRED REAGENT Code Description Quantity

HI93738A-0 Chlorine Dioxide Reagent A 1 mL HI93738B-0 Chlorine Dioxide Reagent B 1 packet HI93738C-0 Chlorine Dioxide Reagent C 1 mL HI93738D-0 Chlorine Dioxide Reagent D 1 mL

REAGENT SETS

HI93738-01 Reagents for 100 tests HI93738-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

SAMPLING PROCEDURE

It is recommended to analyze Chlorine Dioxide samples immediately after collection. Chlorine Dioxide samples must be stored in sealed dark glass bottles, with minimal head space. Excessive heat (above 25 °C / 77 °F), agitation and exposure to light must be avoided.

)

MEASUREMENT PROCEDURE

• Select the Chlorine Dioxide method using the procedure described in the METHOD SELECTION section.

• Fill two graduated mixing cylinders (#1 & #2) up to the 25 mL mark with the sample.

#1 25 ml

#2 25 ml

Page 61

• Add 0.5 mL of HI93738A-0 Chlorine Dioxide Reagent A to each cylinder (#1 & #2), using a 1 mL syringe, cap them and invert several times to mix.

#1 & #2

METHOD PROCEDURES

CHLORINE DIOXIDE

0.5 mL

• Add one packet of HI93738B-0 Chlorine Dioxide Reagent B to one of the two cylinders (#1), cap and invert it several times until it is totally dissolved. This is the blank.

• Add 0.5 mL of HI93738C-0 Chlorine Dioxide Reagent C to each cylinder (#1 & #2), using a 1 mL syringe, cap them and invert several times to mix.

#1 & #2

0.5 mL

Page 62

CHLORINE DIOXIDE

• Add 0.5 mL of HI93738D-0 Chlorine Dioxide Reagent to each cylinder (#1 & #2), using a 1 mL syringe, cap them and invert several times to mix. Cylinder #2 is the reacted sample.

#1 & #2

METHOD PROCEDURES

• Fill cuvette (#1) with 10 mL of the blank (up to the mark). Replace the plastic stopper and the cap.

• Insert the blank (#1) into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Fill second cuvette (#2) with 10 mL of the reacted sample (up to the mark). Replace the plastic stopper and the cap.

0.5 mL

#1 10 mL

#2 10 mL

• Insert the sample into the holder and close the lid.

Page 63

• Press Read to start the reading. The instrument displays the results in mg/L of chlorine dioxide

(ClO2).

INTERFERENCES

Interference may be caused by:

• Strong oxidants

METHOD PROCEDURES

CHLORINE DIOXIDE

Page 64

10.12. CHLORINE DIOXIDE, RAPID METHOD

SPECIFICATIONS

Range 0.00 to 2.00 mg/L (as ClO Resolution 0.01 mg/L Accuracy ±0.10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater,

18th Edition, 4500 ClO2 D

)

METHOD PROCEDURES

CHLORINE DIOXIDE, RAPID METHOD

REQUIRED REAGENT Code Description Quantity

HI96779A-0 Chlorine Dioxide Reagent A 5 drops HI96779B-0 Chlorine Dioxide Reagent B 1 packet

REAGENT SETS

HI96779-01 Reagents for 100 tests HI96779-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

PRINCIPLE

The reaction between the Chlorine Dioxide and DPD indicator causes a pink tint in the sample, the addition of glycine as a masking agent inhibits the response of free chlorine.

APPLICATION

Drinking water, tap water, treated water

SAMPLING PROCEDURE

Collect the sample in a clean glass bottle and analyze it immediately. Chlorine dioxide is a strong oxidizing agent and is unstable in water.

SIGNIFICANCE & USE

Chlorine Dioxide is a commonly-used alternative to chlorine (Cl Chlorophenol Red method (non-rapid method) reacts specifically with chlorine dioxide with little interference from free chlorine or chloramines, but the method procedure is cumbersome. The Chlorine Dioxide Rapid Method based on the DPD (N,N-diethyl-p-phenylenediamine) indicator is a much simpler method by comparison, but it is susceptible to interference from other oxidizers. Glycine (Reagent A) is able to convert free chlorine to chloroaminoacetic acid without affecting the analysis of chlorine dioxide content.

) as a water disinfectant. The

Page 65

MEASUREMENT PROCEDURE

• Select the Chlorine Dioxide (Rapid) method using the procedure described in the METHOD SELECTION section.

METHOD PROCEDURES

CHLORINE DIOXIDE, RAPID METHOD

• Fill the cuvette with 10 mL of unreacted sample (up to the mark).

• Add 5 drops of HI96779A-0 Chlorine Dioxide Reagent A.

• Replace the plastic stopper and the cap. Shake gently for 30 seconds.

• Wait for 30 seconds.

• Insert the cuvette into the holder and close the lid.

10 mL

×5

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Page 66

• Remove the cuvette.

• Add one packet of HI96779B-0 Chlorine Dioxide Reagent B.

METHOD PROCEDURES

• Replace the plastic stopper and the cap. Shake gently for 20 seconds.

• Insert the cuvette into the holder and close the lid.

CHLORINE DIOXIDE, RAPID METHOD

• Press Timer and the display will show the countdown prior to the measurement or wait 1 minute and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of ClO

Page 67

INTERFERENCES

Interference may be caused by:

• Acidity, Alkalinity, Flocculating agents, Hardness, Inorganic and Organic Chloramines, Manganese, Metals, Monochloramine, Oxidized forms of Chromium and Manganese, Ozone and Peroxides

• Chlorine above 5 mg/L

• Bromine above 0.1 mg/L

• Highly buffered samples or extreme sample pH

METHOD PROCEDURES

CHLORINE DIOXIDE, RAPID METHOD

Page 68

10.13. CHLORINE, FREE

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C

CHLORINE, FREE

METHOD PROCEDURES

Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD Method 330.5

REQUIRED REAGENTS

POWDER

Code Description Quantity

HI93701-0 Free Chlorine Reagent 1 packet

LIQUID

Code Description Quantity

HI93701A-F Free Chlorine Reagent A 3 drops HI93701B-F Free Chlorine Reagent B 3 drops

REAGENT SETS

HI93701-F Reagents for 300 tests (liquid) HI93701-01 Reagents for 100 tests (powder) HI93701-03 Reagents for 300 tests (powder)

For other accessories see ACCESSORIES section.

)

MEASUREMENT PROCEDURE

• Select the Chlorine (Free) method using the procedure described in the METHOD SELECTION section.

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

10 mL

Page 69

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

POWDER REAGENT PROCEDURE

• Add the content of one packet of HI93701-0 Free Chlorine Reagent. Replace the plastic stopper and the cap. Shake gently for 20 seconds.

• Insert the cuvette into the holder and close the lid.

METHOD PROCEDURES

CHLORINE, FREE

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LIQUID REAGENT PROCEDURE

• To an empty cuvette add 3 drops of HI93701A-F Free Chlorine Reagent A and 3 drops of HI93701B-F Free Chlorine Reagent B.

×3 ×3

CHLORINE, FREE

• Replace the plastic stopper and the cap. Swirl gently to mix.

METHOD PROCEDURES

• Add 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap. Shake gently.

• Insert the cuvette into the holder and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of chlorine (Cl

Note: Free and Total Chlorine have to be measured separately with fresh sample following the related procedure if both values are desired.

10 mL

INTERFERENCES

Interference may be caused by:

• Bromine, Iodine, Oxidized forms of Chromium and Manganese, Ozone

• Hardness greater than 500 mg/L CaCO

, to remove the interference shake the sample for

approximately 2 minutes after adding the powder reagent

• Alkalinity greater than 250 mg/L CaCO3 or acidity value greater than 150 mg/L CaCO3, the color of the sample may develop only partially or rapidly fade, to remove the interference neutralize the sample with diluted HCl or NaOH

Page 71

10.14. CHLORINE, FREE ULTRA LOW RANGE

SPECIFICATIONS

Range 0.000 to 0.500 mg/L (as Cl Resolution 0.001 mg/L Accuracy ±0.020 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Standard Method 4500-Cl G

REQUIRED REAGENTS Code Description Quantity

HI95762-0 Free Chlorine Ultra Low Range Reagent 1 packet

REAGENTS SETS

HI95762-01 Reagents for 100 tests HI95762-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Chlorine Free ULR method using the procedure described in the METHOD SELECTION section.

)

METHOD PROCEDURES

CHLORINE, FREE ULTRA LOW RANGE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

Page 72

• Remove the cuvette.

• Add one packet of HI95762-0 Free Chlorine ULR Reagent. Replace the plastic stopper and the cap. Shake gently for 20 seconds.

METHOD PROCEDURES

CHLORINE, FREE ULTRA LOW RANGE

• Insert the cuvette into the holder and close the lid.

INTERFERENCES

Interference may be caused by:

• Bromine, Chlorine Dioxide, Iodine, Oxidized forms of Chromium and Manganese, Ozone

• Alkalinity greater than 1000 mg/L CaCO3 if present as bicarbonate (pH < 8.3), above 25 mg/L CaCO3 if present as carbonate (pH > 9.0) or acidity value greater than 150 mg/L CaCO3, the color of the sample may develop only partially or rapidly fade, to remove the interference neutralize the sample with diluted HCl or NaOH

• Hardness greater than 500 mg/L CaCO3, to remove the interference shake the sample for approximately 2 minutes after adding the powder reagent

Page 73

10.15. CHLORINE, TOTAL

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cl Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD Method 330.5

REQUIRED REAGENTS

POWDER

Code Description Quantity

HI93711-0 Total Chlorine Reagent 1 packet

LIQUID

Code Description Quantity

HI93701A-T Total Chlorine Reagent A 3 drops HI93701B-T Total Chlorine Reagent B 3 drops HI93701C-T Total Chlorine Reagent C 1 drop

REAGENT SETS

HI93701-T Reagents for 300 tests (liquid) HI93711-01 Reagents for 100 total tests (powder) HI93711-03 Reagents for 300 total tests (powder)

For other accessories see ACCESSORIES section.

)

METHOD PROCEDURES

CHLORINE, TOTAL

MEASUREMENT PROCEDURE

• Select the Chlorine (Total) method using the procedure described in the METHOD SELECTION section.

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

10 mL

Page 74

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

CHLORINE, TOTAL

• Remove the cuvette.

METHOD PROCEDURES

POWDER REAGENT PROCEDURE

• Add 1 packet of HI93711-0 Total Chlorine Reagent. Replace the plastic stopper and the cap. Shake gently for 20 seconds.

• Insert the cuvette into the holder and close the lid.

Page 75

LIQUID REAGENT PROCEDURE

METHOD PROCEDURES

CHLORINE, TOTAL

• To an empty cuvette add 3 drops of HI93701A-T Total Chlorine Reagent A, 3 drops of HI93701B-T Total Chlorine Reagent B and 1 drop of HI93701C-T Total Chlorine Reagent C.

• Replace the plastic stopper and the cap. Swirl gently to mix.

• Add 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap. Shake gently.

• Insert the cuvette into the holder and close the lid.

×3 ×3 ×1

10 mL

Note: Free and Total Chlorine have to be measured separately with fresh unreacted samples following the related procedure if both values are desired.

Page 76

INTERFERENCES

Interference may be caused by:

• Bromine, Iodine, Oxidized forms of Chromium and Manganese, Ozone

• Hardness greater than 500 mg/L CaCO3, to remove the interference shake the sample for approximately 2 minutes after adding the powder reagent

• Alkalinity greater than 250 mg/L CaCO3 or acidity greater than 150 mg/L CaCO3, the color of the sample may develop only partially or may rapidly fade, to remove the interference neutralize the

CHLORINE, TOTAL

sample with diluted HCl or NaOH

METHOD PROCEDURES

Page 77

10.16. CHLORINE, TOTAL ULTRA LOW RANGE

SPECIFICATIONS

REQUIRED REAGENTS Code Description Quantity

HI95761-0 Total Chlorine Ultra Low Range Reagent 1 packet

REAGENT SETS

HI95761-01 Reagents for 100 tests HI95761-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Chlorine (Total) ULR method using the procedure described in the METHOD SELECTION section.

)

METHOD PROCEDURES

CHLORINE, TOTAL ULTRA LOW RANGE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

Page 78

• Remove the cuvette.

• Add one packet of HI95761-0 Total Chlorine ULR Reagent. Replace the plastic stopper and the cap. Shake gently for 20 seconds.

METHOD PROCEDURES

• Insert the cuvette into the holder and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or wait 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The

CHLORINE, TOTAL ULTRA LOW RANGE

instrument displays the results in mg/L of chlorine (Cl

INTERFERENCES

Interference may be caused by:

• Bromine, Chlorine Dioxide, Iodine, Oxidized forms of Chromium and Manganese, Ozone

• Alkalinity greater than 1000 mg/L CaCO3 if present as bicarbonate (pH < 8.3), above 25 mg/L

if present as carbonate (pH > 9.0) or acidity value greater than 150 mg/L CaCO3, the color

CaCO

of the sample may develop only partially or rapidly fade, to remove the interference neutralize the sample with diluted HCl or NaOH

• Hardness greater than 500 mg/L CaCO approximately 2 minutes after adding the powder reagent

, to remove the interference shake the sample for

Page 79

10.17. CHLORINE, TOTAL ULTRA HIGH RANGE

SPECIFICATIONS

Range 0 to 500 mg/L (as Cl Resolution 1 mg/L Accuracy ±3 mg/L ±3% of reading at 25 °C Light Source LED lamp with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for Examination of Water and Wastewater, 20th Edition, 4500-Cl

REQUIRED REAGENTS Code Description Quantity

HI95771A-0 Total Chlorine Ultra High Range Reagent A 1 packet HI95771B-0 Total Chlorine Ultra High Range Reagent B 1 packet

REAGENTS SETS

HI95771-01 Reagents for 100 tests HI95771-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Chlorine (Total) UHR method using the procedure described in the METHOD SELECTION section.

)

METHOD PROCEDURES

CHLORINE, TOTAL ULTRA HIGH RANGE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

Page 80

• Remove the cuvette.

• Add one packet of HI95771A-0 Total Chlorine Ultra High Range Reagent A and one packet HI95771B-0 Total Chlorine Ultra High Range Reagent B. Replace the plastic stopper and the cap. Shake gently for 20 seconds.

METHOD PROCEDURES

CHLORINE, TOTAL ULTRA HIGH RANGE

• Insert the cuvette into the holder and close the lid.

INTERFERENCES

Interference may be caused by:

• Bromine, Chlorine Dioxide, Chromium, Iodine, Oxidized Manganese, Ozone

Page 81

10.18. CHROMIUM(VI) LOW RANGE

METHOD PROCEDURES

CHROMIUM(VI) LOW RANGE

SPECIFICATIONS

Range 0 to 300 µg/L (as Cr Resolution 1 µg/L Accuracy ±10 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1687 Diphenylcarbohydrazide Method

REQUIRED REAGENTS Code Description Quantity

HI93749-0 Chromium(VI) Low Range Reagent 1 packet

REAGENT SETS

HI93749-01 Reagents for 100 tests HI93749-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Chromium(VI) LR method using the procedure described in the METHOD SELECTION section.

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

(VI))

10 mL

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Page 82

• Remove the cuvette.

• Add one packet of HI93749-0 Chromium(VI) Low Range Reagent. Replace the plastic stopper and the cap. Shake vigorously for about 10 seconds.

• Insert the cuvette into the holder and close the lid.

METHOD PROCEDURES

• Press Timer and the display will show the countdown prior to the measurement or wait 6 minutes

CHROMIUM(VI) LOW RANGE

and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of chromium (Cr

• Press the or  key to access the second level functions.

• Press Chem Frm to convert the result to µg/L of chromate (CrO

¯) and dichromate (Cr2O

• Press the or  key to return to the measurement screen.

INTERFERENCES

Interference may be caused by:

• Vanadium above 1 mg/L, wait 10 minutes before reading to remove the interference

• Iron above 1 mg/L

• Mercurous and mercuric ions slight inhibition of the reaction

¯).

Page 83

10.19. CHROMIUM(VI) HIGH RANGE

SPECIFICATIONS

Range 0 to 1000 µg/L (as Cr(VI)) Resolution 1 µg/L Accuracy ±5 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1687-92, Diphenylcarbohydrazide Method

REQUIRED REAGENTS Code Description Quantity

HI93723-0 Chromium(VI) High Range Reagent 1 packet

REAGENT SETS

HI93723-01 Reagents for 100 tests HI93723-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Chromium(VI) HR method using the procedure described in the METHOD SELECTION section.

METHOD PROCEDURES

CHROMIUM(VI) HIGH RANGE

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL

Page 84

• Remove the cuvette.

• Add one packet of HI93723-0 Chromium(VI) High Range Reagent. Replace the plastic stopper and the cap. Shake vigorously for about 10 seconds.

• Insert the cuvette into the holder and close the lid.

METHOD PROCEDURES

• Press Timer and the display will show the countdown prior to the measurement or wait 6 minutes

CHROMIUM(VI) HIGH RANGE

and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of chromium (Cr

• Press the  or  key to access the second level functions.

• Press Chem Frm to convert the result to µg/L of chromate (CrO

2¯

) and dichromate (Cr2O

• Press the  or  key to return to the measurement screen.

INTERFERENCES

Interference may be caused by:

• Vanadium above 1 mg/L, wait 10 minutes before reading to remove the interference

• Iron above 1 mg/L

• Mercurous and mercuric ions slight inhibition of the reaction

2¯

Page 85

10.20. COLOR OF WATER

SPECIFICATIONS

Range 0 to 500 PCU (Platinum Cobalt Units) Resolution 1 PCU Accuracy ±10 PCU ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18

REQUIRED ACCESSORIES

0.45 µm membrane for true color measurement For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Color of Water method using the procedure described in the METHOD SELECTION section.

Edition, Colorimetric Platinum Cobalt Method

METHOD PROCEDURES

COLOR OF WATER

• Fill the first cuvette (#1) with 10 mL of deionized water (up to the mark). Replace the plastic stopper and the cap. This is the blank.

• Insert the blank (#1) into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

• Remove the cuvette

• Fill the second cuvette (#2) with 10 mL of unfiltered sample (up to the mark). Replace the plastic stopper and the cap. This is the apparent color.

10 mL #1

10 mL #2

Page 86

• Filter 10 mL of sample through a filter with a 0.45 µm membrane into the third cuvette (#3), up to the 10 mL mark. Replace the plastic stopper and the cap. This is the true color.

COLOR OF WATER

METHOD PROCEDURES

• Insert the apparent color cuvette (#2) into the holder and close the lid.

• Press Read to start the reading. The meter displays the value of apparent color in Platinum Cobalt

Units (PCU).

• Remove the apparent color cuvette (#2) from the

holder, insert the true color cuvette (#3) into the holder and close the lid.

• Press Read to start the reading. The meter displays the true color in Platinum Cobalt Units

(PCU).

Page 87

10.21. COPPER LOW RANGE

METHOD PROCEDURES

COPPER LOW RANGE

SPECIFICATIONS

Range 0.000 to 1.500 mg/L (as Cu Resolution 0.001 mg/L Accuracy ±0.010 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA Method

REQUIRED REAGENTS Code Description Quantity

HI95747-0 Copper Low Range Reagent 1 packet

REAGENT SETS

HI95747-01 Reagents for 100 tests HI95747-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Copper LR method using the procedure described in the METHOD SELECTION section.

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

)

10 mL

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Page 88

• Remove the cuvette.

• Add one packet of HI95747-0 Copper Low Range Reagent. Replace the plastic stopper and the cap. Shake gently for about 15 seconds.

COPPER LOW RANGE METHOD PROCEDURES

• Insert the cuvette into the holder and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or wait 45 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of copper (Cu).

INTERFERENCES

Interference may be caused by:

• Cyanide, Silver

• For samples overcoming buffering capacity of reagent around pH 6.8, pH should be adjusted between 6 and 8

Page 89

10.22. COPPER HIGH RANGE

METHOD PROCEDURES

COPPER HIGH RANGE

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cu Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA Method

REQUIRED REAGENTS Code Description Quantity

HI93702-0 Copper High Range Reagent 1 packet

REAGENT SETS

HI93702-01 Reagents for 100 tests HI93702-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Copper HR method using the procedure described in the METHOD SELECTION section.

• Fill the cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

)

10 mL

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

Page 90

• Remove the cuvette.

• Add one packet of HI93702-0 Copper High Range Reagent. Replace the plastic stopper and the cap. Shake gently for about 15 seconds.

COPPER HIGH RANGE METHOD PROCEDURES

• Insert the cuvette into the holder and close the lid.

INTERFERENCES

Interference may be caused by:

• Cyanide, Silver

• For samples overcoming buffering capacity of reagent around pH 6.8, pH should be adjusted between 6 and 8

Page 91

10.23. CYANURIC ACID

SPECIFICATIONS

Range 0 to 80 mg/L (as CYA) Resolution 1 mg/L Accuracy ±1 mg/L ±15% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Turbidimetric Method

REQUIRED REAGENTS Code Description Quantity

HI93722-0 Cyanuric Acid Reagent 1 packet

REAGENT SETS

HI93722-01 Reagents for 100 tests HI93722-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Cyanuric Acid method using the procedure described in the METHOD SELECTION section.

METHOD PROCEDURES

CYANURIC ACID

• Fill the first cuvette with 10 mL of unreacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL # 1

# 1

Page 92

• Fill a beaker with 25 mL sample.

CYANURIC ACID

• Add one packet of HI93722-0 Cyanuric Acid Reagent and mix to dissolve.

METHOD PROCEDURES

• Fill a second cuvette with 10 mL of the reacted sample (up to the mark). Replace the plastic stopper and the cap.

• Insert the cuvette into the holder and close the lid.

# 2 10 mL

# 2

Page 93

10.24. FLUORIDE LOW RANGE

METHOD PROCEDURES

FLUORIDE LOW RANGE

SPECIFICATIONS

Range 0.00 to 2.00 mg/L (as F Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th Edition, SPADNS Method

REQUIRED REAGENT Code Description Quantity

HI93729-0 Fluoride Low Range Reagent 4 mL

REAGENT SETS

HI93729-01 Reagents for 100 tests HI93729-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Fluoride LR method using the procedure described in the METHOD SELECTION section.

• Add 2 mL of HI93729-0 Fluoride Low Range Reagent to two cuvettes.

)

2 mL

• Use a plastic pipette to fill one cuvette with 10 mL of deionized water (up to the mark) (#1). Replace the plastic stopper and the cap. Invert several times to mix.

• Use a plastic pipette to fill the second cuvette with 10 mL of unreacted sample (up to the mark) (#2). Replace the plastic stopper and the cap. Invert several times to mix.

• Insert the first cuvette (#1) into the holder and close the lid.

10 mL # 1

# 1

10 mL # 2

Page 94

• Press Timer and the display will show the countdown prior to zeroing the blank or wait 2 minutes and press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

METHOD PROCEDURES

FLUORIDE LOW RANGE

• Remove the cuvette.

• Insert the second cuvette (#2) with the reacted sample into the holder and close the lid.

• Press Read to start reading. The instrument displays the results in mg/L of fluoride (F

Note: For wastewater or seawater samples, before performing measurements, distillation is required. For most accurate results use two graduated pipettes to deliver exactly 8 mL of deionized water and 8 mL of sample.

INTERFERENCES

Interference may be caused by:

• Alkalinity above 5000 mg/L CaCO

• Chloride above 700 mg/L

• Sulfate above 200 mg/L

• Orthophosphate above 16 mg/L

• Iron (Ferric) above 10 mg/L

• Sodium hexametaphosphate above 1.0 mg/L

• Aluminum above 0.1 mg/L

• Highly colored and turbid samples may require distillation

• Highly alkaline samples can be neutralized with nitric acid

# 2

Page 95

10.25. FLUORIDE HIGH RANGE

METHOD PROCEDURES

FLUORIDE HIGH RANGE

SPECIFICATIONS

Range 0.0 to 20.0 mg/L (as F Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th Edition, SPADNS Method

REQUIRED REAGENT Code Description Quantity

HI93739A-0 Fluoride High Range Reagent A 2 mL HI93739B-0 Fluoride High Range Reagent B 8 mL

REAGENT SETS

HI93739-01 Reagents for 100 tests HI93739-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Fluoride HR method using the procedure described in the METHOD SELECTION section.

)

• Use 1 mL syringe one and add 2 mL of HI93739A-0 Fluoride High Range Reagent A to the cuvette. Use the pipette to fill up the cuvette to the 10 mL mark with HI93739B-0 Fluoride High Range Reagent B.

• Replace the plastic stopper and the cap. Invert several times to mix.

• Insert the cuvette into the holder and close the lid.

2 mL

10 mL

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• Press Timer and the display will show the countdown prior to zeroing the blank or wait 1 minute and press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

METHOD PROCEDURES

FLUORIDE HIGH RANGE

• Remove the cuvette.

• Add 1 mL of sample to the cuvette using the second 1 mL syringe.

• Replace the plastic stopper and the cap. Invert several times to mix.

• Insert the cuvette into the holder and close the lid.

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Note: For wastewater or seawater samples, before performing measurements, distillation is required.

INTERFERENCES

Interference may be caused by:

• Alkalinity above 5000 mg/L CaCO

• Chloride above 700 mg/L

• Sulfate above 200 mg/L

• Orthophosphate above 16 mg/L

• Aluminum above 0.1 mg/L

• Iron (Ferric) above 10 mg/L

• Sodium hexametaphosphate above 1.0 mg/L

• Highly colored and turbid samples may require distillation

• Highly alkaline samples can be neutralized with nitric acid

METHOD PROCEDURES

FLUORIDE HIGH RANGE

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10.26. HARDNESS, CALCIUM

SPECIFICATIONS

Range 0.00 to 2.70 mg/L (as CaCO Resolution 0.01 mg/L Accuracy ±0.11 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th Edition, Calmagite Method

HARDNESS, CALCIUM METHOD PROCEDURES

REQUIRED REAGENTS Code Description Quantity

HI93720A-0 Calcium Hardness Reagent A 0.5 mL HI93720B-0 Calcium Hardness Reagent B 0.5 mL HI93720C-0 Calcium Hardness Reagent C 1 drop

REAGENT SETS

HI93720-01 Reagents for 100 tests HI93720-03 Reagents for 300 tests

For other accessories see ACCESSORIES section.

MEASUREMENT PROCEDURE

• Select the Hardness (Calcium) method using the procedure described in the METHOD SELECTION section.

)

• Rinse a graduated beaker several times with unreacted sample, before filling it to the 50 mL mark with the sample.

• Add 0.5 mL of HI93720A-0 Calcium Hardness Reagent A and swirl to mix the solution.

• Add 0.5 mL of HI93720B-0 Calcium Hardness Reagent B and swirl to mix the solution. Fill two cuvettes with 10 mL of sample (up to the mark).

# 1

# 2

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×1

• Add 1 drop of HI93720C-0 Calcium Hardness Reagent C to one cuvette (#1).

# 1

• Replace the plastic stopper and the cap. Invert the cuvette several times to mix. This is the blank.

# 1

• Insert the blank (#1) into the holder and close the lid.

• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement.

METHOD PROCEDURES

HARDNESS, CALCIUM

# 2

• Remove the blank (#1) and insert the second cuvette (#2) into the holder.

• Press Read to start the reading. The instrument displays concentration in mg/L of calcium

carbonate (CaCO

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• Press the  or  key to access the second level functions.

• Press Chem Frm to convert the result to French degrees (°f), German degrees (°dH) and

English degrees (°E).

HARDNESS, CALCIUM METHOD PROCEDURES

• Press the  or  key to return to the measurement screen.

Note: This test will detect any calcium contamination in the beaker, measuring syringes or sample cells. To test cleanliness repeat the test multiple times until you obtain consistent results.

SAMPLE DILUTION

This meter is designed to determine low levels of hardness, typically found in water purification systems. Dilutions must be performed with hardness-free water or the readings will be erroneous. To reduce the level of hardness by a factor of one hundred:

• Fill a 1 mL syringe with the sample.

• Add 0.5 mL of sample to a clean, dry 50 mL beaker

• Fill the beaker up to the 50 mL mark with hardness-free water.

100

INTERFERENCES

Interference may be caused by:

• Excessive amounts of heavy metals