Hanna Instruments HI83300 Instruction Manual

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INSTRUCTION MANUAL

HI83300

Multiparameter

Photometer

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Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for correct use of the

instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e‑mail us at

tech@hannainst.com or view our worldwide contact list at www.hannainst.com.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

Dear Customer,

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TABLE OF CONTENTS

1. PRELIMINARY EXAMINATION ............................................................................................

2. SAFETY MEASURES...................... ....................................................................................

3. SPECIFICATIONS ..............................................................................................................

4. DESCRIPTION..... .............................................................................................................

4.1. GENERAL DESCRIPTION.............................................................................................

4.2. PRECISION AND ACCURACY........................................................................................

4.3. FUNCTIONAL DESCRIPTION........................................................................................

4.4. PRINCIPLE OF OPERATION.........................................................................................

4.5. OPTICAL SYSTEM......................................................................................................

5. GENERAL OPERATIONS......... ............................................................................................

5.1. POWER CONNECTION AND BATTERY MANAGEMENT......................................................

5.2. GENERAL SETUP........................................................................................................

5.3. USING HANNA DIGITAL ELECTRODES...........................................................................

5.4. MODE SELECTION.....................................................................................................

5.5. LOGGING DATA.........................................................................................................

5.6. ADDING SAMPLE / USER NAMES TO LOG DATA............................................................

5.7. DATA MANAGEMENT..................................................................................................

5.8. CONTEXTUAL HELP....................................................................................................

6. PHOTOMETER MODE......... ...............................................................................................

6.1. METHOD SELECTION.................................................................................................

6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS..............................................

6.2.1. PROPER USE OF SYRINGE...................................................................................

6.2.2. PROPER USE OF DROPPER...................................................................................

6.2.3. PROPER USE OF POWDER PACKET.........................................................................

6.3. CUVETTE PREPARATION.............................................................................................

6.4. TIMERS AND MEASUREMENT FUNCTIONS...................................................................

6.5. CHEMICAL FORMULA / UNIT CONVERSIONS................................................................

6.6. METER VALIDATION / CAL CHECK................................................................................

6.7. ABSORBANCE MEASUREMENTS..................................................................................

7. PROBE MODE................... ...............................................................................................

7.1. pH CALIBRATION......................................................................................................

7.2. pH CALIBRATION MESSAGES......................................................................................

7.3. pH MEASUREMENT...................................................................................................

7.4. pH MEASUREMENT MESSAGES / WARNINGS...............................................................

7.5. pH GLP.....................................................................................................................

7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE.....................................................

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TABLE OF CONTENTS

8. METHOD PROCEDURES........ ........................................................................................

8.1. ALKALINITY...............................................................................................................

8.2. ALKALINITY, MARINE.................................................................................................

8.3. ALUMINUM..............................................................................................................

8.4. AMMONIA LOW RANGE..............................................................................................

8.5. AMMONIA MEDIUM RANGE........................................................................................

8.6. AMMONIA HIGH RANGE.............................................................................................

8.7. BROMINE.................................................................................................................

8.8. CALCIUM................................................................................................................

8.9. CALCIUM, MARINE..................................................................................................

8.10. CHLORIDE..............................................................................................................

8.11. CHLORINE DIOXIDE.................................................................................................

8.12. CHLORINE, FREE.....................................................................................................

8.13. CHLORINE, FREE ULTRA LOW RANGE.........................................................................

8.14. CHLORINE, TOTAL...................................................................................................

8.15. CHLORINE, TOTAL ULTRA LOW RANGE.......................................................................

8.16. CHLORINE, TOTAL ULTRA HIGH RANGE......................................................................

8.17. CHROMIUM (VI) LOW RANGE...................................................................................

8.18. CHROMIUM (VI) HIGH RANGE..................................................................................

8.19. COLOR OF WATER...................................................................................................

8.20. COPPER LOW RANGE...............................................................................................

8.21. COPPER HIGH RAGE................................................................................................

8.22. CYANURIC ACID......................................................................................................

8.23. FLUORIDE LOW RANGE............................................................................................

8.24. FLUORIDE HIGH RANGE...........................................................................................

8.25. HARDNESS, CALCIUM..............................................................................................

8.26. HARDNESS, MAGNESIUM........................................................................................

8.27. HARDNESS, TOTAL LOW RANGE................................................................................

8.28. HARDNESS, TOTAL MEDIUM RANGE..........................................................................

8.29. HARDNESS, TOTAL HIGH RANGE...............................................................................

8.30. HYDRAZINE

............................................................................................................

8.31. IODINE..................................................................................................................

8.32. IRON LOW RANGE...................................................................................................

8.33. IRON HIGH RANGE..................................................................................................

8.34. MAGNESIUM..........................................................................................................

8.35. MANGANESE LOW RANGE........................................................................................

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TABLE OF CONTENTS

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8.36. MANGANESE HIGH RANGE......................................................................................

8.37. MOLYBDENUM.......................................................................................................

8.38. NICKEL LOW RANGE................................................................................................

8.39. NICKEL HIGH RANGE...............................................................................................

8.40. NITRATE................................................................................................................

8.41. NITRITE, MARINE ULTRA LOW RANGE.......................................................................

8.42. NITRITE LOW RANGE...............................................................................................

8.43. NITRITE HIGH RANGE..............................................................................................

8.44. OXYGEN, DISSOLVED...............................................................................................

8.45. OXYGEN SCAVENGERS (CARBOHYDRAZIDE)...............................................................

8.46. OXYGEN SCAVENGERS (DIETHYLHYDROXYLAMINE) (DEHA)........................................

8.47. OXYGEN SCAVENGERS (HYDROQUINONE).................................................................

8.48. OXYGEN SCAVENGERS (ISO‑ASCORBIC ACID)............................................................

8.49. OZONE...................................................................................................................

8.50. pH........................................................................................................................

8.51. PHOSPHATE, MARINE ULTRA LOW RANGE.................................................................

8.52. PHOSPHATE LOW RANGE.........................................................................................

8.53. PHOSPHATE HIGH RANGE........................................................................................

8.54. POTASSIUM...........................................................................................................

8.55. SILICA LOW RANGE.................................................................................................

8.56. SILICA HIGH RANGE................................................................................................

8.57. SILVER..................................................................................................................

8.58. SULFATE................................................................................................................

8.59. SURFACTANTS, ANIONIC..........................................................................................

8.60. ZINC......................................................................................................................

9. ERROR DESCRIPTIONS......... ............................................................................................

10. STANDARD METHODS......... ...........................................................................................

11. ACCESSORIES................................................................................................................

11.1. REAGENT SETS.......................................................................................................

11.2. pH ELECTRODES.....................................................................................................

11.3. pH SOLUTIONS.....................................................................................................

11.4. OTHER ACCESSORIES..............................................................................................

12. ABBREVIATIONS............................................................................................................

RECOMMENDATIONS FOR USERS..........................................................................................

WARRANTY.........................................................................................................................

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1. PRELIMINARY EXAMINATION

Remove the instrument and accessories from the packaging and examine it carefully to make sure that no damage has occurred during shipping. Notify your nearest Hanna Customer Service Center if damage is observed.

Each HI83300 is supplied with:

• Sample Cuvette and Cap (4 pcs.)

• Cloth for Wiping Cuvettes

• Scissors

• USB Cable

• 5 Vdc Power Adapter

• Instruction Manual

• DO bottle (glass stopper bottle)

• Quality Certificate

Note: Save all packing material until you are sure that the instrument works correctly. Any damaged or defective item must be returned in its original packing material with the supplied accessories.

2. SAFETY MEASURES

•The chemicals contained in the reagent kits may be hazardous if improperly handled.

•Read the Safety Data Sheets (SDS) before performing tests.

• Safety equipment: Wear suitable eye protection and clothing when required, and follow instructions carefully.

• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released vapors.

• Waste disposal: for proper disposal of reagent kits and reacted samples, contact a licensed waste disposal provider.

PRELIMINARY EXAMINATIONSAFETY MEASURES

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Measurement Channels

5 x optical channels; 1 x digital electrode channel (pH measurement)

Absorbance

Range 0.000 to 4.000 Abs Resolution 0.001 Abs Accuracy ±0.003 Abs (at 1.000 Abs) Light Source light emitting diode Bandpass Filter Bandwidth 8 nm

Bandpass Filter Wavelength Accuracy ±1.0 nm

Light Detector silicon photocell Cuvette Types round, 24.6 mm diameter Number of Methods 65

Range ‑2.00 to 16.00 pH (± 1000.0 mV)* Resolution 0.01 pH (0.1 mV) Accuracy ±0.01 pH (±0.2 mV) (@ 25 °C / 77 °F) Temperature Compensation ATC (‑5.0 to 100.0 °C; 23.0 to 212.0 °F)*

Calibration

2 points, eligible from 5 available buffers (4.01,

6.86, 7.01, 9.18, 10.01 pH)

Electrode Intelligent pH / temperature electrode

Temperature

Range ‑20.0 to 120.0°C (‑4.0 to 248.0 °F) Resolution 0.1 °C (0.1 °F) Accuracy ±0.5 °C (±0.9 °F ) (@ 25 °C / 77 °F)

Additional Specifications

Logging 1000 readings (mixed photometer and electrode) Display 128 x 64 pixel B/W LCD with backlight USB‑A (Host) Functions mass‑storage host USB‑B (Device) Functions power input, mass‑storage device

Battery Life

> 500 photometer measurements, or 50 hours of continuous pH measurement

Power Supply

5 Vdc USB 2.0 power adapter/type micro‑B connector

3.7 Vdc Li‑polymer rechargeable battery, non‑serviceable

Environment

0 to 50 °C (32 to 122 °F); 0 to 95% RH, non‑serviceable

Dimensions 206 x 177 x 97 mm (8.1 x 7.0 x 3.8") Weight 1.0 kg (2.2 lbs.)

3. SPECIFICATIONS

*Limits will be reduced to actual probe/sensor limits.

SPECIFICATIONS

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DESCRIPTION

4. DESCRIPTION

4.1.GENERAL DESCRIPTION HI83300 multiparameter photometer is compact and versatile meter with two measurement

modes: Absorbance and pH/ mV. Absorbance mode include CAL Check feature and 65 different methods that cover a wide variety of applications, making it ideal for both benchtop and portable operation.

• Digital electrode input for pH measurements

• Certified CAL Check cuvettes to confirm meter functionality

• Dual purpose micro‑USB flash drive

• Li‑polymer rechargeable battery

• Auto‑off

• Absorbance mode

• User and sample name entry

• GLP features

4.2.PRECISION AND ACCURACY

Precision is how closely repeated measurements are to one another. Precision is usually expressed as standard deviation (SD). Accuracy is defined as the closeness of a test result to the true value. Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these definitions. For each method, the accuracy is expressed in the related measurement section.

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DESCRIPTION

1) Splash‑proof keypad

2) Liquid Crystal Display (LCD)

3) Indexing mark

4) Protective port covers

5) Light‑blocking cover panel

6) Cuvette holder

7) ON/OFF power button

8) 3.5 mm TRRS (jack) input for digital electrodes

9) Standard USB host connector for data transfer to a USB flash drive

10) Micro‑USB device connector for power or PC interface

4.3. FUNCTIONAL DESCRIPTION

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DESCRIPTION

Keypad Description

The keypad contains 12 direct keys and 3 functional keys with the following functions:

Press the functional keys to perform the function displayed above them on the LCD.

Press to access the list of photometer methods.

Press to move up in a menu or a help screen, to increment a set value, or to access second level functions.

Press to toggle between photometer and pH (electrode) mode.

Press to move left in a menu or to decrement a set value.

Press to move down in a menu or a help screen, to decrement a set value, or to access second level functions.

Press to move right in a menu or to increment a set value.

Press to access the setup screen.

Press to log the current reading.

Press to review saved logs.

Press to exit the current screen.

Press to display the help screen.

ON/OFF power button.

4.4. PRINCIPLE OF OPERATION

Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical‑chemical characteristics of the substance according to the Lambert‑Beer Law:

‑log I/I

= el c d

A = e

c d

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DESCRIPTION

Instrument Block Diagram

The internal reference system (reference detector) of the HI83300 photometer compensates for any drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero) measurement and sample measurement. LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher luminous efficiency, providing more light while using less power. They also produce little heat, which could otherwise affect electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps have poor blue/violet light output. Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to be received. The end result is higher measurement stability and less wavelength error. A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette imperfections and scratches, eliminating the need to index the cuvette.

4.5. OPTICAL SYSTEM

 = intensity of incident light beam

I = intensity of light beam after absorption e

l

= molar extinction coefficient at wavelength l c = molar concentration of the substance d = optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are constant. Photometric chemical analysis is based on specific chemical reactions between a sample and reagent to produce a light‑absorbing compound.

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5. GENERAL OPERATIONS

5.1. POWER CONNECTION AND BATTERY MANAGEMENT

The meter can be powered from an AC/DC adapter (included) or from the built‑in rechargeable battery. The meter will perform an auto‑diagnostic test when it is first powered on. During this test, the HANNA® logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display. The battery icon on the LCD will indicate the battery status:

‑ battery is charging from external adapter ‑ battery fully charged (meter connected to AC/DC adapter)

‑ battery capacity (no external adapter) ‑ battery near 0% (no external adapter)

‑ battery at 0% (no external adapter)

To conserve battery, the meter will turn off automatically after 15 minutes of inactivity (30 minutes before a READ measurement). If a photometer measurement is on the screen, an auto‑log is created before shutdown.

5.2. GENERAL SETUP

Press SETUP key to enter in Setup menu, highlight desired option using and press Select.

CAL Check (Photometer Only)

Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are displayed on the screen. To start a new CAL Check press Check key and follow the prompts on the screen.

GENERAL OPERATIONS

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GENERAL OPERATIONS

Backlight

Values: 0 to 8

Press the Modify key to access the backlight intensity. Use the functional keys or the   keys to increase or decrease the value. Press the Accept key to confirm or ESC to return to the Setup menu without saving the new value.

Contrast

Values: 0 to 20

Press the Modify key to change the display’s contrast. Use the functional keys or the   keys to increase or decrease the value. Press the Accept key to confirm the value or ESC to return to the Setup menu without saving the new value.

Date / Time

Press the Modify key to change the date/time. Press the functional keys or the   keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the   keys to change the value. Press the Accept key to confirm or ESC to return to the Setup without saving the new date or time.

Time Format

Option: AM/PM or 24-hour Press the functional key to select the desired time format.

Temperature Unit (pH Only)

Option: °C or °F

Press the functional key to select the desired temperature unit.

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GENERAL OPERATIONS

Date Format

Press the Modify key to change the Date Format. Use the   keys to select the desired format. Press the Select key to confirm or ESC to return to the Setup menu without saving the new format.

Decimal Separator

Option: Comma ( , ) or Period ( . ) Press the functional key to select the desired decimal separator. The decimal separator is used on the measurement screen and CSV files.

Language

Press the Modify key to change the Language. Use the   keys to select the desired language. Press Select to choose one of the 7 languages installed.

Beeper

Option: Enable or Disable

When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error is detected. Press the functional key to enable/disable the beeper.

Instrument ID

Option: 0 to 999999

This option is used to set the instrument’s ID (identification number). Press the Modify key to access the instrument ID screen. Use the functional keys or the  keys to highlight the digit to be modified. Press the   keys in order to set the desired value. Press the Accept key to confirm the value or ESC to return to the Setup menu without saving the new value.

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GENERAL OPERATIONS

Meter Information

Press the Select key to view the model, serial number, firmware version and selected language. Press ESC to return to the Setup menu.

Probe Information (pH mode only)

Press the Select key to view model number, serial number and firmware version for the connected probe. Press ESC to return to the Setup menu.

5.3. USING HANNA DIGITAL ELECTRODES

The HI83300 can be used to perform direct pH measurements by connecting a HANNA® digital pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the electrode to the 3.5 mm port marked with “EXT PROBE” located at the rear of the meter. If the meter is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.

5.4. MODE SELECTION

The HI83300 has two operational modes: Photometer Mode and Probe Mode. Photometer Mode enables on‑demand measurement of a cuvette using the integrated optical system. Photometric‑related functions, such as Method selection, Zero, Read, and Timers are available in this mode.

Probe Mode enables continuous measurement using a Hanna Digital Electrode connected to the

3.5 mm port. Probe‑related functions, such as calibration and GLP, are available in this mode. To switch between Photometer Mode and Probe Mode, use the

button.

Note: The active mode cannot be switched while in menus, such as Setup, Recall, Method, etc.

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5.5. LOGGING DATA

The instrument features a data log function to help you keep track of all your analysis. The data log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the LOG and RECALL keys. Storing data: You can store only a valid measurement. Press LOG and the last valid measurement will be stored with date and time stamp.

5.6. ADDING SAMPLE / USER NAMES TO LOG DATA

A sample ID and user ID can be added to the saved log. Use the  keys to highlight the Sample ID or User ID then press Modify.

Text Entry

Sample ID and User ID care entered using the alphanumeric multi‑tapping keypad.

Enter one character at a time by pressing the key with the assigned character repeatedly until the desired character is highlighted. For reference, a list of the characters available for the current key will be shown under the text box. The character will be entered after a two‑second delay or after another key is pressed.

Once all characters have been entered, press Accept to use the displayed text.

GENERAL OPERATIONS

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GENERAL OPERATIONS

The following functions are available during Text Entry:

• Accept: Press to accept the current displayed text.

• Arrow: Press to delete the last character.

• Clear: Press to delete all characters.

Press to discard all changes and return to the previous screen.

Viewing and deleting: You can view, export and delete the data by pressing the RECALL key. Use the  keys to scroll through the saved logs. Press Info to view additional information about the selected log.

Data Export:

Log data can be exported to a USB flash drive or to a PC. To access Data Export functions, press Recall then Export.

Use the   keys to select the desired export location. For export to USB Flash Drive, insert the USB Flash Drive into the dedicated port at the back of the meter labeled HOST USB, then follow the on‑screen prompts.

For export to PC, connect the meter to a PC using the supplied micro‑USB cable. Insert the cable into the port at the back of the meter labeled PC PWR. Follow the on‑screen prompts. When the meter says PC connected, use a file manager (such as Windows Explorer or Mac Finder) to move the file from the meter to the PC. The meter will appear as a removable disk.

5.7. DATA MANAGEMENT

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5.8. CONTEXTUAL HELP HI83300 offers an interactive contextual help mode that assists the user at any time.

To access the help screen press HELP. The instrument will display additional information related to the current screen. To read all the available information, scroll the text using the   keys. To exit help mode press ESC key and the meter will return to the previous screen.

Log data is exported as a single file containing all logged photometer and probe data. The file name is: “HI83300.csv”. The CSV file (Comma‑Separated Values) may be opened with a text editor or spreadsheet application.

6. PHOTOMETER MODE

6.1. METHOD SELECTION

In order to select the desired method press the METHOD key and a screen with the available methods will appear. Press the  keys to highlight the desired method. Press Select.

After the desired method is selected, follow the procedure described in the related section. Before performing a method read all the instructions carefully.

GENERAL OPERATIONSPHOTOMETER MODE

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PHOTOMETER MODE

6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS

6.2.1. PROPER USE OF SYRINGE

(a) Push the plunger completely into the syringe and insert the tip

into the solution.

(b) Pull the plunger up until the lower edge of the seal is exactly on

the mark for the desired volume.

be sure that no drops are hanging on the tip of the syringe. Then, keeping the syringe in vertical position above the cuvette, push the plunger down into the syringe, the desired volume has been delivered into the cuvette.

6.2.2. PROPER USE OF DROPPER

(a) For reproducible results, tap the dropper on the table several times and wipe the outside of the

tip with a cloth.

(b) Always keep the dropper bottle in a vertical position while dosing the reagent.

6.2.3. PROPER USE OF POWDER PACKET

(a) Use scissors to open the powder packet (b) Push the edges of the packet to form a spout (c) Pour out the content of the packet.

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6.3. CUVETTE PREPARATION

Proper mixing is very important for reproducibility of the measurements. The proper mixing technique for each method is listed in the method procedure. (a) Invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical

position. Turn the cuvette upside‑down and wait for all of the solution to flow to the cap end, then return the cuvette to the upright vertical position and wait for all of the solution to flow to the cuvette bottom. This is one inversion. The correct speed for this mixing technique is 10‑15 complete inversions in 30 seconds.

This mixing technique is indicated with “invert to mix” and the following icon:

(b) Shaking the cuvette, moving the cuvette up and down. The movement may be gentle or

vigorous. This mixing method is indicated with “shake gently” or “shake vigorously”, and one of the following icons:

In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied HDPE plastic stopper

and then the black cap. Whenever the cuvette is placed into the measurement holder, it must be dry outside and free of fingerprints, oil or dirt. Wipe it thoroughly with HI731318 or a lint‑free cloth prior to insertion. Shaking the cuvette can generate bubbles in the sample, causing higher readings. To obtain accurate measurements, remove such bubbles by swirling or by gently tapping the cuvette.

shake gently shake vigorously

PHOTOMETER MODE

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PHOTOMETER MODE

Do not let the reacted sample stand too long after reagent is added. For best accuracy, respect the timings described in each specific method.

It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for each sample and to use the same cuvette for zeroing and measurement when possible. Discard the sample immediately after the reading is taken, or the glass might become permanently stained. All the reaction times reported in this manual are at 25 °C (77 °F). In general, the reaction time should be increased for temperatures lower than 20 °C (68 °F), and decreased for temperatures higher than 25 °C (77 °F).

Interference

In the method measurement section the most common interferences that may be present in a typical water sample have been reported. It is possible that a particular application could introduce other compounds that will also interfere.

6.4. TIMERS AND MEASUREMENT FUNCTIONS

Each method requires a different preparation procedure, reaction times, sample preparations, etc. If a timer or timers are necessary for proper sample preparation, the Timer key will be available.

To use a reaction timer, press the Timer key.

The default timer will start immediately. To stop and reset the timer, press Stop.

If the selected method requires more than one timer, the meter will automatically select each timer in the appropriate order. To bypass the default order, you may press the desired key to activate a different timer (only while the current timer is stopped). Press Continue to start the active timer.

For some methods, the timer is only necessary after a Zero measurement has been performed. In this case, the timer key will only be available after the Zero measurement has been performed.

If the method requires a Zero or Read measurement after a timer has expired, the meter will automatically perform the appropriate action. Follow the instructions in the Method Procedure.

To perform a Zero or Read measurement, insert the appropriate prepared cuvette, then press the Zero or Read key. A Zero measurement must be conducted before Read measurements.

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6.5. CHEMICAL FORMULA / UNIT CONVERSION

Chemical formula/unit conversion factors are pre‑programmed into the instrument and are method specific. In order to view the displayed result in the desired chemical formula press  keys to access the second level function and then press the Chem Frm key to toggle between the available chemical formulas for the selected method.

6.6. METER VALIDATION / CAL CHECK

WARNING: Do not validate the meter with standard solutions other than the HANNA

CAL Check

Standards. For accurate validation results, please perform tests at room temperature (18 to 25 °C;

64.5 to 77.0 °F).

Validation of the HI83300 involves absorbance measurements of certified HANNA

CAL Check Standards (see “Accessories”). The “CAL Check” screen guides the user through the measurement of each CAL Check Standard and applies the factory calibration corrections to each measurement. The

HI83300 stores the results of the most recent CAL Check measurements which may be viewed on

the “CAL Check” screen. Compare these results with the values printed on the Certificate provided with each HANNA® CAL Check Standards kit.

To perform a validation:

1. Press Setup button.

2. Highlight CAL Check, then press Select.

3. Follow the prompts on the screen. The meter will prompt to measure each cuvette provided in the HANNA

CAL Check

Standards kit. To abort the process at any time, press ESC button.

PHOTOMETER MODE

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4. Press ESC to return in Setup menu.

6.7. ABSORBANCE MEASUREMENTS

Raw absorbance measurements may be performed on the HI83300 for personal or diagnostic purposes. For example, you may monitor the stability of a reagent blank by occasionally measuring its absorbance versus deionized water.

To measure the raw absorbance of a prepared sample:

1. Enable “Photometer Mode” if necessary by pressing the MODE key.

2. Press the METHOD key.

3. Highlight the appropriate Absorbance method (according to the wavelength to be used), then press Select. Absorbance methods are located at the bottom of the method list.

4. Prepare the sample cuvette according to the method.

5. Insert a cuvette filled with deionized water, then press Zero.

6. Insert the prepared sample cuvette, then press Read.

WARNING: Never use Absorbance methods for validation using HANNA

CAL Check cuvettes. The

factory calibration corrections for CAL Check cuvettes are applied while in CAL Check mode only!

PHOTOMETER MODE

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7. PROBE MODE

7.1. pH CALIBRATION

Press MODE to enter in pH/ mV measurement mode.

Press Calibrate to access electrode calibration functions.

Calibration Mode

While in pH Calibration Mode, the display will show the current pH reading, the current temperature reading, the current selected buffer, and the buffer number (“Buffer: 1” for the 1st buffer, “Buffer: 2” for the 2nd buffer).

The following functions are available in pH Calibration Mode:

• Clear: Press to clear the current calibration from the probe.

• Confirm: Press to accept the current calibration point. Only

available if the measurement is stable and within the limits for the selected buffer.

Press to cycle through the list of available buffers: 4.01, 6.86, 7.01, 9.18, 10.01 pH.

Press to exit calibration and return to pH Measurement Mode.

PROBE MODE

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PROBE MODE

Preparation

Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences. For accurate calibration and to minimize cross‑contamination, use two beakers for each buffer solution: one for rinsing the electrode and one for calibration. If you are measuring in the acidic range, use pH 7.01 or 6.86 as the first buffer and pH 4.01 as the second buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as the first buffer and pH 10.01 or 9.18 as the second buffer.

Procedure

Calibration can be performed using one or two calibration buffers. For more accurate measurements, a two‑point calibration is recommended. Submerse the pH electrode approximately 3 cm (1¼”) into a buffer solution and stir gently. From the Probe Measurement screen, press the Calibrate key to begin the calibration process.

When the reading is stable and close to the selected buffer, the Confirm key will become available. Press Confirm to accept and store the calibration point.

The meter will now prompt for the second buffer (“Buffer: 2”). To use only a one‑point calibration, press

to exit calibration mode at this time. The meter will store the calibration information to the probe and return to Measurement mode. To continue calibrating with a second buffer, rinse and submerse the pH electrode approximately 3 cm (1¼”) into the second buffer solution and stir gently. If necessary, press

keys to select a different buffer value.

When the reading is stable and close to the selected buffer, the Confirm key will become available. Press Confirm to accept and store the second calibration point.

The meter will store the two‑point calibration information to the probe and return to Measurement mode. The list of calibrated buffers will appear at the bottom of the screen.

Page 26

7.2. pH CALIBRATION MESSAGES

Clean Probe:

The "Clean Probe" message indicates poor electrode performance (offset out of accepted window, or slope under the accepted lower limit). Often, cleaning the probe will improve the pH electrode's response. See pH Electrode Conditioning and Maintenance for details. Repeat calibration after cleaning.

Check Probe & Buffer:

The “Check Probe & Buffer” message appears when there is a large difference between the pH measurement and the selected buffer value, or the electrode slope is outside of the accepted slope limit. You should check your probe and confirm the correct buffer selection. Cleaning may also improve this response.

Wrong Temperature:

The buffer temperature is too extreme for the selected buffer value.

7.3. pH MEASUREMENT

The HI83300 can be used to perform direct pH measurements by connecting a HANNA

digital pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter. If the meter is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.

While taking pH probe measurements, the following functions are available:

• Calibrate: Press to access electrode calibration functions.

• GLP: Press to review the last calibration information, including date/time, buffers used, slope,

and offset.

• Range: Press to switch between “pH” units and “mV” units.

PROBE MODE

Page 27

Press to switch to Photometer mode.

Press to access the meter’s Setup menu.

Press to log the current measurement.

Press to review the meter’s log history.

Press to view contextual help information.

For high accuracy it is recommended to calibrate your electrode often. pH electrodes should be recalibrated at least once per week, but daily calibration is recommended. Always recalibrate after cleaning an electrode. See page 24 for more information on pH calibration.

To take pH measurements:

• Remove the protective cap and rinse the electrode with water.

• Collect some sample in a clean, dry beaker.

• Preferably, rinse the electrode with a small amount of sample. Discard the rinse.

• Submerse the electrode tip approximately 3 cm (1¼”) into the sample to be tested and stir the sample gently. Make sure the electrode junction is completely submersed.

• Allow time for the electrode to stabilize in the sample. When the

symbol disappears, your

reading is stable.

If measurements are taken successively in different samples, it is recommended to rinse the electrodes thoroughly with deionized or distilled water and then with some of the next sample to prevent cross‑contamination.

pH measurements are affected by temperature. HANNA

Digital pH electrodes include a built‑in temperature sensor and automatically calculate corrected pH values. The measured temperature is displayed on the screen with the pH measurements.

PROBE MODE

Page 28

PROBE MODE

7.4. pH MEASUREMENT MESSAGES / WARNINGS

No Probe:

No probe is connected or the probe is broken.

Connecting:

The meter has detected a probe and is reading the probe configuration and calibration information.

Incompatible Probe:

The connected probe is not compatible with this device.

Incompatible Calibration:

The probe’s current calibration is not compatible with this meter. The calibration must be cleared to use this probe.

Exceeded Probe Range:

The pH and/or temperature measurement exceed the specifications of the probe. The affected measurement value(s) will be flashing.

Broken Temperature Sensor:

The temperature sensor inside the probe is broken. Temperature compensation will revert to a fixed value of 25 °C (77 °C).

Cal Due:

The probe has no calibration. See section Probe Calibration.

Page 29

PROBE MODE

7.5. pH GLP

Good Laboratory Practice (GLP) refers to a quality control function used to ensure uniformity and consistency of sensor calibrations and measurements. To view the GLP information, press the GLP key from the Probe Measurement screen.

The pH GLP screen displays the following information about the last pH calibration:

• Date and time of the last calibration

• List of buffers used in the last calibration

• Calculated slope and offset

• Press ESC to return in measurement mode.

Page 30

7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE

Remove the protective cap of the pH electrode. DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode. These bubbles can be removed by “shaking down” the electrode as you would do with a glass thermometer. If the bulb and/or junction is dry, soak the electrode in HI70300 or HI80300 storage solution for at least one hour.

For refillable electrodes: If the fi lling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI7082 or

HI8082 3.5M KCl Electrolyte Solution for double junction electrodes.

Unscrew the fill hole cover during measurements so the liquid reference junction maintains an outward + ow of electrolyte.

Measurement

Rinse the electrode tip with distilled water. Submerse the tip 3 cm (1¼”) in the sample and stir gently for a few seconds. For a faster response and to avoid cross‑contamination of the samples, rinse the electrode tip with a few drops of the solution to be tested, before taking measurements.

PROBE MODE

Page 31

Storage Procedure

To minimize clogging and ensure a quick response time, the glass bulb and the junction should be kept moist and not allowed to dry out.

Replace the solution in the protective cap with a few drops of HI70300 or HI80300 Storage Solution or, in its absence, Filling Solution (HI7082 or HI8082 for double junction electrodes). Follow the preparation procedure before taking measurements.

Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.

Periodic Maintenance

Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable or cracks on the electrode stem or bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the electrode. Rinse off any salt deposits with water. For refillable electrodes: Refill the reference chamber with fresh electrolyte (HI7082 or HI8082 for double junction electrodes). Allow the electrode to stand upright for 1 hour. Follow the Storage Procedure above.

Cleaning Procedure

Use diagnostic messages to aid pH electrode troubleshooting. Several cleaning solutions are available:

• General – Soak in Hanna HI7061 or HI8061 General Cleaning Solution for approximately ½ hour.

• Protein –Soak in Hanna HI7073 or HI8073 Protein Cleaning Solution for 15 minutes.

• Inorganic – Soak in Hanna HI7074 Inorganic Cleaning Solution for 15 minutes.

• Oil/grease – Rinse with Hanna HI7077 or HI8077 Oil and Fat Cleaning Solution.

Note: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel- filled electrodes) and soak the electrode in HI70300 or HI80300 Storage Solution for at least 1 hour before taking measurements.

Temperature Correlation For pH Sensitive Glass

Verify the temperature range by reading the limits on electrodes cap. The pH electrode’s life also depends on the temperature that is used. If constantly cycled between two temperatures, the life of the electrode is drastically reduced.

PROBE MODE

Page 32

10 mL

8. METHOD PROCEDURES

8.1. ALKALINITY

SPECIFICATIONS

Range 0 to 500 mg/L (as CaCO3) Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method.

REQUIRED REAGENTS Code Description Quantity

HI775S Alkalinity Reagent 1 mL HI93755‑53 Chlorine Removal Reagent 1 drop

REAGENT SETS

HI775‑26 Reagents for 25 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Alkalinity method using the procedure described in the

Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

ALKALINITY

Page 33

ALKALINITY

• Remove the cuvette.

Note: Any chlorine present in the sample will interfere with the reading. To remove the chlorine interference add one drop of HI93755-53 Chlorine Removal Reagent to the unreacted sample.

• Add 1mL of HI775S Alkalinity Reagent to the sample using a 1 mL syringe.

• Replace the cap and invert 5 times.

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L calcium carbonate

(CaCO

Page 34

10 mL

8.2. ALKALINITY, MARINE

SPECIFICATIONS

Range 0 to 300 mg/L (as CaCO3) Resolution 1 mg/L Accuracy ±5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Colorimetric Method.

REQUIRED REAGENTS Code Description Quantity

HI755S Alkalinity Reagent 1 mL

REAGENT SETS

HI755‑26 Reagents for 25 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Alkalinity Marine method using the procedure described in the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

ALKALINITY, MARINE

Page 35

• Remove the cuvette.

• Add 1 mL of HI755S Alkalinity Reagent to the sample using a 1mL syringe.

• Replace the cap and gently invert 5 times.

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L as calcium

carbonate (CaCO

• Press  or  to access the second level functions.

• Press Chem Frm key to convert the result to degree KH (dKH).

• Press  or  to return to the measurement screen.

ALKALINITY, MARINE

Page 36

8.3. ALUMINUM

SPECIFICATIONS

Range 0.00 to 1.00 mg/L (as Al3+) Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the aluminon method.

REQUIRED REAGENTS Code Description Quantity

HI93712A‑0 Aluminum Reagent A 1 packet HI93712B‑0 Aluminum Reagent B 1 packet HI93712C‑0 Aluminum Reagent C 1 packet

REAGENT SETS

HI93712‑01 Reagents for 100 tests HI93712‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Aluminum method using the procedure described in the Method Selection section (see page 18).

• Fill a graduated beaker with 50 mL of sample.

• Add one packet of HI93712A‑0 Aluminum Reagent A and mix until completely dissolved.

• Add one packet of HI93712B‑0 Aluminum Reagent B and mix until completely dissolved.

ALUMINUM

Page 37

ALUMINUM

• Fill two cuvettes with 10 mL of sample (up to the mark).

• Add one packet of HI93712C‑0 Aluminum Reagent C to one cuvette (#1). Replace the cap and shake gently until

completely dissolved. This is the blank.

• Place the first cuvette (#1) into the holder and close the lid.

• Press Timer and the display will show the countdown prior to zeroing the blank. Alternatively wait for 15 minutes and then press Zero. The display will show “‑0.0‑“ when the meter is zeroed and ready for measurement.

• Remove the blank and insert the second cuvette (#2) into the instrument and close the lid.

10 mL

# 1

# 2

# 1

# 2

Page 38

• Press the Read key to start the reading. The instrument displays the results in mg/L of

aluminum (Al

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to mg/L of aluminum oxide (Al

2O3

• Press  or  to return to the measurement screen.

INTERFERENCES Interference may be caused by: Iron above 20 mg/L Alkalinity above 1000 mg/L Phosphate above 50 mg/L Fluoride must be absent

ALUMINUM

Page 39

AMMONIA LOW RANGE

8.4. AMMONIA LOW RANGE

SPECIFICATIONS

Range 0.00 to 3.00 mg/L (as NH3‑N) Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426 Nessler method.

REQUIRED REAGENTS Code Description Quantity

HI93700A‑0 Ammonia Low Range Reagent A 4 drops HI93700B‑0 Ammonia Low Range Reagent B 4 drops

REAGENT SETS

HI93700‑01 Reagents for 100 tests HI93700‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Ammonia LR method using the procedure described in the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

10 mL

Page 40

• Add 4 drops of HI93700A‑0 Ammonia Low Range Reagent A. Replace the cap and mix the solution.

• Add 4 drops of HI93700B‑0 Ammonia Low Range Reagent B. Replace the cap and mix the solution.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH

-N).

AMMONIA LOW RANGE

Page 41

AMMONIA LOW RANGE

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium

(NH

• Press  or  to return to the measurement screen.

INTERFERENCE

Interference may be caused by: Acetone Alcohols Aldehydes Glycine Hardness above 1 g/L Iron Organic chloramines Sulfide Various aliphatic and aromatic amines

Page 42

10 mL

8.5. AMMONIA MEDIUM RANGE

SPECIFICATIONS

Range 0.00 to 10.00 mg/L (as NH

‑N) Resolution 0.01 mg/L Accuracy ±0.05 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler method.

REQUIRED REAGENTS Code Description Quantity

HI93715A‑0 Ammonia Medium Range Reagent A 4 drops HI93715B‑0 Ammonia Medium Range Reagent B 4 drops

REAGENT SETS

HI93715‑01 Reagents for 100 tests HI93715‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Ammonia MR method using the procedure described in the

Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

AMMONIA MEDIUM RANGE

Page 43

AMMONIA MEDIUM RANGE

• Remove the cuvette.

• Add 4 drops of HI93715A‑0 Ammonia Medium Range

Reagent A. Replace the cap and mix the solution.

• Add 4 drops of HI93715B‑0 Ammonia Medium Range

Reagent B. Replace the cap and mix the solution.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results to mg/L of ammonia nitrogen (NH

-N).

• Press or to access the second level functions.

Page 44

AMMONIA MEDIUM RANGE

• Press the Chem Frm key to convert the result in mg/L of ammonia (NH3) and ammonium

(NH

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Acetone Alcohols Aldehydes Glycine Hardness above 1 g/L Iron Organic chloramines Sulfide Various aliphatic and aromatic amines

Page 45

AMMONIA HIGH RANGE

8.6. AMMONIA HIGH RANGE

SPECIFICATIONS

Range 0.0 to 100.0 mg/L (as NH3‑N) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler method.

REQUIRED REAGENTS Code Description Quantity

HI93733A‑0 Ammonia High Range Reagent A 4 drops HI93733B‑0 Ammonia High Range Reagent B 9 mL

REAGENT SETS

HI93733‑01 Reagents for 100 tests HI93733‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Ammonia HR method using the procedure described in the

Method Selection section (see page 18).

• Add 1mL of unreacted sample to the cuvette using 1mL

syringe.

• Use the pipette to fill the cuvette up to the 10 mL mark

with HI93733B‑0 Ammonia High Range Reagent B. Replace the cap and mix the solution.

• Place the cuvette into the holder and close the lid.

1 mL

Page 46

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Add 4 drops of HI93733A‑0 Ammonia High Range Reagent A.

Replace the cap and swirl the solution.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH

-N).

• Press  or  to access the second level functions.

AMMONIA HIGH RANGE

Page 47

AMMONIA HIGH RANGE

•Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium

(NH

•Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Acetone Alcohols Aldehydes Glycine Hardness above 1 g/L Iron Organic chloramines Sulfide Various aliphatic and aromatic amines

Page 48

8.7. BROMINE

SPECIFICATIONS

Range 0.00 to 8.00 mg/L (as Br2) Resolution 0.01 mg/L Accuracy ±0.08 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, DPD method.

REQUIRED REAGENTS Code Description Quantity

HI93716‑0 Bromine Reagent 1 packet

REAGENT SETS

HI93716‑01 Reagents for 100 tests HI93716‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Bromine method using the procedure described in the

Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

10 mL

BROMINE

Page 49

BROMINE

• Remove the cuvette.

• Add one packet of HI93716‑0 Bromine Reagent. Replace

the cap and shake gently for about 20 seconds to dissolve most of the reagent.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of bromine (Br

INTERFERENCES

Interference may be caused by: Chlorine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO

, shake the sample for approximately 1 minute after adding the reagent. In case of water with alkalinity greater than 300 mg/L CaCO3 or acidity greater than 150 mg/L CaCO3, the color of the sample may develop only partially, or may rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

Page 50

3 mL of sample

8.8. CALCIUM

SPECIFICATIONS

Range 0 to 400 mg/L (as Ca2+) Resolution 1 mg/L Accuracy ±10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Oxalate method.

REQUIRED REAGENTS Code Description Quantity

‑ Buffer Reagent 4 drops HI93752A‑Ca Calcium Reagent A 7 mL HI93752B‑Ca Calcium Reagent B 1 mL

REAGENT SETS

HI937521‑01 Reagents for 50 tests HI937521‑03 Reagents for 150 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Calcium method using the procedure described in the

Method Selection section (see page 18).

• Add 3 mL of unreacted sample to the cuvette using the 5 mL syringe.

• Use the pipette to fill the cuvette up to the 10 mL

mark with the HI93752A‑Ca Calcium Reagent A.

• Add 4 drops of Buffer Reagent.

CALCIUM

Page 51

• Replace the cap and invert several times to mix.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Add 1 mL of HI93752B‑Ca Calcium Reagent B

to the sample using the 1 mL syringe. Invert the cuvette 10 times to mix (about 15 seconds).

• Reinsert the cuvette into the instrument.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 5 minutes.

CALCIUM

Page 52

• After waiting 5 minutes, invert again the cuvette 10 times

to mix (about 15 seconds).

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca

INTERFERENCES

Interferences may be caused by: Acidity (as CaCO

) above 1000 mg/L

Alkalinity (as CaCO

) above 1000 mg/L

Magnesium (Mg

) above 400 mg/L

CALCIUM

Page 53

10 mL

8.9. CALCIUM, MARINE

SPECIFICATIONS

Range 200 to 600 mg/L (as Ca2+) Resolution 1 mg/L Accuracy ±6% of reading at 25 °C Light Source LED with narrow band interference filter @ 610 nm Method Adaptation of the Zincon method.

REQUIRED REAGENTS Code Description Quantity

HI7581 Calcium Reagent A 1 mL HI7582 Calcium Reagent B 1 packet

REAGENT SETS

HI758‑26 Reagents for 25 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Calcium Marine method using the procedure described in

the Method Selection section (see page 18).

• Add 1 mL of HI7581 Calcium Reagent A to the cuvette

using a 1 mL syringe.

• Use the plastic pipette to fill the cuvette to the 10 mL mark

with deionized water and replace the cap. Invert 3‑5 times to mix.

• Place the cuvette into the holder and close the lid.

CALCIUM, MARINE

Page 54

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Use the minipipette to add 0.1 mL of sample to the cuvette.

• Add one packet of HI7582 Calcium Reagent B. Replace

the cap and shake vigorously for 15 seconds or until the powder is completely dissolved. Allow air bubbles to dissipate for 15 seconds before taking a reading.

• Reinsert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca

CALCIUM, MARINE

Page 55

10 mL

8.10. CHLORIDE

SPECIFICATIONS

Range 0.0 to 20.0 mg/L (as Cl‑) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±6% of reading at 25 °C Light Source Light Emitting Diode with narrow band interference filter @ 466 nm Method Adaptation of the mercury(II) thiocyanate method.

REQUIRED REAGENTS Code Description Quantity

HI93753A‑0 Chloride Reagent A 1 mL HI93753B‑0 Chloride Reagent B 1 mL

REAGENT SETS

HI93753‑01 Reagents for 100 tests HI93753‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chloride method using the procedure described in the Method Selection section (see

page 18).

• Fill one cuvette (#1) with 10 mL of deionized water (up to the mark).

• Fill another cuvette (#2) with 10 mL of sample (up to the mark).

Notes: • For samples with low chloride ion concentration, rinse the cuvette a few times with sample before filling it with 10 mL of sample.

• For the most accurate results, use two graduated pipettes to deliver exactly10 mL of deionized water and 10 mL of sample to the cuvettes.

10 mL

CHLORIDE

Page 56

• Add 0.5 mL of HI93753A‑0 Chloride Reagent A to each cuvette using the 1 mL syringe

• Replace the caps and mix each cuvette by inverting for approximately 30 seconds.

• Add 0.5 mL of HI93753B‑0 Chloride Reagent B to each cuvette using the second 1 mL syringe.

• Replace the caps and mix each cuvette by inverting for approximately 30 seconds.

• Place the cuvette with the reacted deionized water (#1) into the holder and close the lid.

#1 #2

CHLORIDE

Page 57

• Press Timer and the display will show the countdown prior to the zero or, alternatively, wait for 2 minutes and press Zero. The display will show "‑0.0‑" when the meter is zeroed and ready for measurement.

• Remove the cuvette.

• Insert the other cuvette (# 2) with the reacted sample into the instrument and close the lid.

• Press Read to start reading. The instrument displays the results in mg/L of chloride (Cl

CHLORIDE

Page 58

INTERFERENCES

Interference may be caused by: For alkaline samples, neutralize before adding reagents. The pH of the sample after addition of reagents should be about 2. Intensely colored samples will cause interference, therefore they should be adequately treated before performing the test. Suspended matter in large amount should be removed by prior filtration.

CHLORIDE

Page 59

25 ml

8.11. CHLORINE DIOXIDE

SPECIFICATIONS

Range 0.00 to 2.00 mg/L (as ClO2) Resolution 0.01 mg/L Accuracy ±0.10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the Chlorophenol Red method.

REQUIRED REAGENT Code Description Quantity

HI93738A‑0 Chlorine Dioxide Reagent A 1 mL HI93738B‑0 Chlorine Dioxide Reagent B 1 packet HI93738C‑0 Chlorine Dioxide Reagent C 1 mL HI93738D‑0 Chlorine Dioxide Reagent D 1 mL

REAGENT SETS

HI93738‑01 Reagents for 100 tests HI93738‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chlorine Dioxide method using the procedure described in the Method Selection section (see page 18).

• Fill two graduated mixing cylinders (#1 & #2) up to the 25 mL mark with the sample.

• Add 0.5 mL of HI93738A‑0 Chlorine Dioxide Reagent A to each cylinder (#1 & #2), using a 1 mL syringe, cap them and invert several times to mix.

25 ml

#1 #2

#1 & #2

CHLORINE DIOXIDE

Page 60

CHLORINE DIOXIDE

• Add one packet of HI93738B‑0 Chlorine Dioxide Reagent B to one of the two cylinders (#1), cap and invert it several times until it is totally dissolved. This is the blank.

• Add 0.5 mL of HI93738C‑0 Chlorine Dioxide Reagent C to each cylinder (#1 & #2), using a 1 mL syringe, cap, and invert several times to mix.

• Add 0.5 mL of HI93738D‑0 Chlorine Dioxide Reagent to each cylinder (#1 & #2), using a 1 mL syringe, cap and invert several times to mix. Cylinder #2 is the reacted sample.

• Fill cuvette (#1) with 10 mL of the blank (up to the mark) and replace the cap.

• Place the blank (#1) into the holder and close the lid.

#1 & #2

#1 #2

#1 10 mL

#1 & #2

Page 61

CHLORINE DIOXIDE

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Fill second cuvette (#2) with 10 mL of the reacted sample (up to the mark) and replace the cap.

• Insert the sample into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of chlorine

dioxide (ClO

SAMPLING PROCEDURE

It is recommended to analyze chlorine dioxide samples immediately after collection. Chlorine Dioxide samples must be stored in sealed dark glass bottle, with minimal head space. Excessive heat (above 25 °C /77 °F), agitation and exposure to light must be avoided.

INTERFERENCES

Interferences may be caused by strong oxidants.

#2 10 mL

Page 62

8.12. CHLORINE, FREE

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cl

) Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD method 330.5.

REQUIRED REAGENTS

POWDER:

Code Description Quantity

HI93701‑0 Free Chlorine Reagent 1 packet

LIQUID:

Code Description Quantity

HI93701A‑F Free Chlorine Reagent A 3 drops HI93701B‑F Free Chlorine Reagent B 3 drops

REAGENT SETS

HI93701‑F Reagents for 300 tests (liquid) HI93701‑01 Reagents for 100 tests (powder) HI93701‑03 Reagents for 300 tests (powder)

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chlorine (Free) method using the procedure described in

the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

and replace the cap.

• Place the cuvette into the holder and close the lid.

10 mL

CHLORINE, FREE

Page 63

CHLORINE, FREE

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

POWDER REAGENT PROCEDURE

• Add the content of one packet of HI93701‑0 Free Chlorine

Reagent. Replace the cap and shake gently for 20 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or alternatively,

wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of chlorine (Cl

LIQUID REAGENT PROCEDURE

• To an empty cuvette add 3 drops of HI93701A‑F Free

Chlorine Reagent A and 3 drops of HI93701B‑F Free Chlorine Reagent B.

Page 64

CHLORINE, FREE

• Insert the cuvette into the instrument and close the lid.

• Press Read to start the reading. The instrument displays the results in mg/L of chlorine (Cl

Note: Free and Total Chlorine have to be measured separately with fresh sample following the related procedure if both values are desired.

INTERFERENCES

Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO

, shake the sample for approximately 2 minutes after adding the powder reagent. If the water used for this procedure has an alkalinity value greater than 250 mg/L CaCO3 or acidity value greater than 150 mg/L CaCO3, the color of the sample may develop only partially, or may rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

10 mL

• Swirl gently to mix.

• Add 10 mL of unreacted sample (up to the mark).

Replace the cap and shake gently.

Page 65

8.13. CHLORINE, FREE ULTRA LOW RANGE

SPECIFICATIONS

Range 0.000 to 0.500 mg/L (as Cl2) Resolution 0.001 mg/L Accuracy ±0.020 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Standard Method 4500‑Cl G.

REQUIRED REAGENTS Code Description Quantity

HI95762‑0 Free Chlorine Ultra Low Range Reagent 1 packet

REAGENTS SETS

HI95762‑01 Reagents for 100 tests HI95762‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chlorine Free ULR method using the procedure

described in the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the

mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

10 mL

CHLORINE, FREE ULTRA LOW RANGE

Page 66

CHLORINE, FREE ULTRA LOW RANGE

• Add one packet of HI95762‑0 Free Chlorine Reagent.

Replace the cap and shake gently for 20 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of chlorine (Cl

INTERFERENCES

Interference may be caused by: Alkalinity: above 1,000 mg/L CaCO

if present as bicarbonate (HCO3 sample pH < 8.3); above

25 mg/L CaCO

if present as carbonate (CO

2‑

, sample pH > 9.0). In both cases, it will not reliably develop the full amount of color or it may rapidly fade (negative error). To resolve this, neutralize the sample with diluted HCl. Acidity: above 150 mg/L CaCO

. May not reliably develop the full amount of color or it may rapidly fade (negative error). To resolve this, neutralize the sample with diluted NaOH. Hardness: in case of water with hardness greater than 500 mg/L CaCO

, shake the sample for approximately 2 minutes after adding the powder reagent. Bromine (Br

), Chloride dioxide (ClO2), Iodine (I2), Oxidized Manganese and Chromium, Ozone

): positive error.

Page 67

8.14. CHLORINE, TOTAL

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cl2) Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD method 330.5.

REQUIRED REAGENTS

POWDER:

Code Description Quantity

HI93711‑0 Total Chlorine Reagent 1 packet

LIQUID:

Code Description Quantity

HI93701A‑T Total Chlorine Reagent A 3 drops HI93701B‑T Total Chlorine Reagent B 3 drops HI93701C‑T Total Chlorine Reagent C 1 drop

REAGENT SETS

HI93701‑T Reagents for 300 tests (liquid) HI93711‑01 Reagents for 100 total tests (powder) HI93711‑03 Reagents for 300 total tests (powder)

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chlorine (Total) method using the procedure described in

the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

and replace the cap.

• Place the cuvette into the holder and close the lid.

10 mL

CHLORINE, TOTAL

Page 68

CHLORINE, TOTAL

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

POWDER REAGENT PROCEDURE

• Add 1 packet of HI93711‑0 Total Chlorine Reagent. Replace the

cap and shake gently for 20 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of chlorine (Cl

LIQUID REAGENT PROCEDURE

• To an empty cuvette add 3 drops of HI93701A‑T

Total Chlorine Reagent A, 3 drops of HI93701B‑T Total Chlorine Reagent B, and 1 drop of

HI93701C‑T Total Chlorine Reagent C. Swirl gently

to mix.

Page 69

CHLORINE, TOTAL

• Insert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of chlorine (Cl

Note: Free and Total Chlorine have to be measured separately with fresh unreacted samples following the related procedure if both values are desired.

INTERFERENCES

Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO

shake the sample for approximately 2 minutes after adding the powder reagent. If the water used for this procedure has an alkalinity value greater than 250 mg/L CaCO3 or acidity value greater than 150 mg/L CaCO

, the color of the sample may develop only partially, or may

rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.

10 mL

• Add 10 mL of unreacted sample (up to the mark).

Replace the cap and shake gently.

Page 70

8.15. CHLORINE, TOTAL ULTRA LOW RANGE

SPECIFICATIONS

REQUIRED REAGENTS Code Description Quantity

HI95761‑0 Total Chlorine Ultra Low Range Reagent 1 packet

REAGENT SETS

HI95761‑01 Reagents for 100 tests HI95761‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chlorine (Total) ULR method using the procedure

described in the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the

mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

10 mL

CHLORINE, TOTAL ULTRA LOW RANGE

Page 71

CHLORINE, TOTAL ULTRA LOW RANGE

• Add one packet of HI95761‑0 Total Chlorine Reagent.

Replace the cap and shake gently for 20 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of chlorine (Cl

INTERFERENCES

Interference may be cased by: Alkalinity: above 1,000 mg/L CaCO

if present as bicarbonate (HCO3 sample pH < 8.3); above

25 mg/L CaCO3 if present as carbonate (CO

2‑

, sample pH > 9.0). In both cases, it will not reliably develop the full amount of color or it may rapidly fade (negative error). To resolve this, neutralize the sample with diluted HCl.

Acidity: above 150 mg/L CaCO

. May not reliably develop the full amount of color or it may rapidly

fade (negative error). To resolve this, neutralize the sample with diluted NaOH. Hardness: in case of water with hardness greater than 500 mg/L CaCO

, shake the sample for approximately 2 minutes after adding the powder reagent. Bromine (Br2), Ozone (O3) and Chlorine dioxide (ClO2): positive error.

Page 72

8.16. CHLORINE, TOTAL ULTRA HIGH RANGE

SPECIFICATIONS

Range 0 to 500 mg/L (as Cl2) Resolution 1 mg/L Accuracy ±3 mg/L ±3% of reading at 25 °C Light Source LED lamp with narrow band interference filter @ 525 nm Method Adaptation of the Standard Methods for Examination of Water and Wastewater, 20th edition, 4500‑Cl.

REQUIRED REAGENTS Code Description Quantity

HI95771A‑0 Total Chlorine Ultra High Range Reagent A 1 packet HI95771B‑0 Total Chlorine Ultra High Range Reagent B 1 packet

REAGENTS SETS

HI95771‑01 Reagents for 100 tests HI95771‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chlorine (Total) UHR method using the procedure

described in the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the

mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

10 mL

CHLORINE, TOTAL ULTRA HIGH RANGE

Page 73

• Add one packet of HI95771A‑0 Total Chlorine Ultra High

Range Reagent A and one packet HI95771B‑0 Total Chlorine Ultra High Range Reagent B. Replace the cap and shake gently for 20 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of chlorine (Cl

INTERFERENCES

Interference may be caused by: Bromine (Br

), Oxidized Manganese, Chromium, Chlorine Dioxide (ClO2), Ozone (O3) and Iodine (I2).

CHLORINE, TOTAL ULTRA HIGH RANGE

Page 74

8.17. CHROMIUM (VI) LOW RANGE

SPECIFICATIONS

Range 0 to 300 µg/L (as Cr (VI)) Resolution 1 µg/L Accuracy ±10 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1687 Diphenylcarbohydrazide method.

REQUIRED REAGENTS Code Description Quantity

HI93749‑0 Chromium (VI) Low Range Reagent 1 packet

REAGENT SETS

HI93749‑01 Reagents for 100 tests HI93749‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chromium (VI) LR method using the procedure described

in the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Add one packet of HI93749‑0 Chromium(VI) Low Range

Reagent. Replace the cap and shake vigorously for about

10 seconds.

10 mL

CHROMIUM (VI) LOW RANGE

Page 75

CHROMIUM (VI) LOW RANGE

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of chromium (Cr

• Press or  to access the second level functions.

• Press the Chem Frm key to convert the result to µg/L of chromate (CrO

2¯

) and dichromate

(Cr2O

2¯

• Press  or  to return to the measurement screen.

INTERFERENCES

Interference may be caused by: Vanadium above 1 ppm. However, waiting 10 minutes before reading removes the interference. Iron above 1 ppm. Mercurous and mercuric ions cause slight inhibition of the reaction.

Page 76

8.18. CHROMIUM (VI) HIGH RANGE

SPECIFICATIONS

Range 0 to 1000 µg/L (as Cr(VI)) Resolution 1 µg/L Accuracy ±5 µg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1687, Diphenylcarbohydrazide method.

REQUIRED REAGENTS Code Description Quantity

HI93723‑0 Chromium(VI) High Range Reagent 1 packet

REAGENT SETS

HI93723‑01 Reagents for 100 tests HI93723‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Chromium(VI) HR method using the procedure described

in the Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette.

• Add one packet of HI93723‑0 Chromium(VI) High Range

Reagent. Replace the cap and shake vigorously for about 10 seconds.

10 mL

CHROMIUM (VI) HIGH RANGE

Page 77

CHROMIUM (VI) HIGH RANGE

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in µg/L of chromium (Cr

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the result to µg/L of chromate (CrO

2¯

) and dichromate

(Cr2O

2¯

• Press  or  to return to the measurement screen.

INTERFERENCES

Page 78

8.19. COLOR OF WATER

SPECIFICATIONS

Range 0 to 500 PCU (Platinum Cobalt Units) Resolution 1 PCU Accuracy ±10 PCU ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Colorimetric Platinum Cobalt method.

REQUIRED ACCESSORIES

0.45 µm membrane for true color measurement. For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Color of Water method using the procedure described in

the Method Selection section (see page 18).

• Fill the first cuvette (#1) up to the mark with deionized water and

replace the cap.

• Place the blank (#1) into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the cuvette

10 mL

COLOR OF WATER

Page 79

COLOR OF WATER

• Fill the second cuvette (#2) up to the mark with unfiltered

sample and replace the cap. This is the apparent color.

• Filter 10 mL of sample through a filter with a 0.45 µm

membrane into the third cuvette (#3), up to the 10 mL mark and replace the cap. This is the true color.

• Insert the apparent color cuvette (#2) into the instrument

and close the lid.

• Press Read to start the reading. The meter displays the value of apparent color in Platinum

Cobalt Units (PCU).

• Remove the apparent color cuvette(#2) from the

instrument, insert the true color cuvette (#3) into the instrument and close the lid.

•Press Read to start the reading. The meter displays the true color in Platinum Cobalt Units

(PCU).

10 mL

Page 80

8.20. COPPER LOW RANGE

SPECIFICATIONS

Range 0.000 to 1.500 mg/L (as Cu) Resolution 0.001 mg/L Accuracy ±0.010 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA method.

REQUIRED REAGENTS Code Description Quantity

HI95747‑0 Copper Low Range Reagent 1 packet

REAGENT SETS

HI95747‑01 Reagents for 100 tests HI95747‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Copper LR method using the procedure described in the

Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

10 mL

COPPER LOW RANGE

Page 81

COPPER LOW RANGE

• Remove the cuvette.

• Add one packet of HI95747‑0 Copper Low Range Reagent.

Replace the cap and shake gently for about 15 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 45 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of copper (Cu).

INTERFERENCES

Interference may be caused by: Silver, Cyanide. For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted between 6 and 8.

Page 82

8.21. COPPER HIGH RANGE

SPECIFICATIONS

Range 0.00 to 5.00 mg/L (as Cu) Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the EPA method.

REQUIRED REAGENTS Code Description Quantity

HI93702‑0 Copper High Range Reagent 1 packet

REAGENT SETS

HI93702‑01 Reagents for 100 tests HI93702‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Copper HR method using the procedure described in the

Method Selection section (see page 18).

• Fill the cuvette with 10 mL of unreacted sample (up to the mark)

and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

10 mL

COPPER HIGH RANGE

Page 83

COPPER HIGH RANGE

• Remove the cuvette.

• Add one packet of HI93702‑0 Copper High Range Reagent.

Replace the cap and shake gently for about 15 seconds.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 45 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of copper (Cu).

INTERFERENCES

Interference may be caused by: Silver, Cyanide. For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted between 6 and 8.

Page 84

8.22. CYANURIC ACID

SPECIFICATIONS

Range 0 to 80 mg/L (as CYA) Resolution 1 mg/L Accuracy ±1 mg/L ±15% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the turbidimetric method.

REQUIRED REAGENTS Code Description Quantity

HI93722‑0 Cyanuric Acid Reagent 1 packet

REAGENT SETS

HI93722‑01 Reagents for 100 tests HI93722‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Cyanuric Acid method using the procedure described in the

Method Selection section (see page 18).

• Fill the first cuvette with 10 mL of unreacted

sample (up to the mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

# 1

10 mL

# 1

CYANURIC ACID

Page 85

CYANURIC ACID

• Add one packet of HI93722‑0 Cyanuric Acid Reagent and

mix to dissolve.

• Fill a second cuvette with 10 mL of the reacted sample,

up to the mark, and replace the cap.

• Reinsert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for 45 seconds and press Read. When the timer ends the meter will perform the reading. The instrument displays the concentration in mg/L of cyanuric acid.

• Fill a beaker with 25 mL sample (up to the mark).

10 mL

# 2

Page 86

8.23. FLUORIDE LOW RANGE

SPECIFICATIONS

Range 0.00 to 2.00 mg/L (as F‑) Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, SPADNS method.

REQUIRED REAGENT Code Description Quantity

HI93729‑0 Fluoride Low Range Reagent 4 mL

REAGENT SETS

HI93729‑01 Reagents for 100 tests HI93729‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Fluoride LR method using the procedure

described in the Method Selection section (see page 18).

• Add 2 mL of HI93729‑0 Fluoride Low Range Reagent to

two cuvettes.

• Use a plastic pipette to fill the cuvette to the 10 mL

mark with deionized water (#1), replace the cap and invert several times to mix.

• Use a plastic pipette to fill the second cuvette to

the 10 mL mark with unreacted sample (#2), replace the cap and invert several times to mix.

• Place the first cuvette (#1) into the holder and close the lid.

# 1

10 mL

# 1

# 2

2 mL

FLUORIDE LOW RANGE

Page 87

FLUORIDE LOW RANGE

• Press Timer and the display will show the countdown prior to zeroing the blank or, alternatively,

wait for two minutes and press Zero. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

• Insert the second cuvette (#2) with the reacted sample into the instrument and close the lid.

• Press Read to start reading. The instrument displays the results in mg/L of fluoride (F

Note: For wastewater or seawater samples, before performing measurements, distillation is required. For most accurate results use two graduated pipettes to deliver exactly 8 mL of deionized water and 8 mL of sample.

INTERFERENCES

Interferences may be caused by: Alkalinity (as CaCO

) above 5000 mg/L Aluminum above 0.1 mg/L Iron, ferric above 10 mg/L Chloride above 700 mg/L ortho‑Phosphate above 16 mg/L Sodium hexametaphosphate above 1.0 mg/L Sulfate above 200 mg/L Highly colored and turbid samples may require distillation Highly alkaline samples can be neutralized with nitric acid.

# 2

Page 88

8.24. FLUORIDE HIGH RANGE

SPECIFICATIONS

Range 0.0 to 20.0 mg/L (as F‑) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 575 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, SPADNS method.

REQUIRED REAGENT Code Description Quantity

HI93739A‑0 Fluoride High Range Reagent A 2 mL HI93739B‑0 Fluoride High Range Reagent B 8 mL

REAGENT SETS

HI93739‑01 Reagents for 100 tests HI93739‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Fluoride HR method using the procedure

described in the Method Selection section

(see page 18).

• Add 2.00 mL of HI93739A‑0 Fluoride High Range

Reagent A to the cuvette and use the pipette to fill up the cuvette to the 10 mL mark with HI93739B‑0 Fluoride High Range Reagent B.

• Replace the cap and invert several times to mix.

• Place the cuvette into the holder and close the lid.

FLUORIDE HIGH RANGE

2 mL

10 mL

Page 89

FLUORIDE HIGH RANGE

• Press Timer and the display will show the countdown prior to zeroing the blank or, alternatively,

wait for one minute and press Zero. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the cuvette.

• Add 1 mL of sample to the cuvette using the second 1 mL syringe.

• Replace the cap and invert several times to mix.

• Insert the cuvette into the instrument and close the lid.

• Press Timer and the display will show the countdown prior to the measurement or, alternatively,

wait for one minute and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of fluoride (F

Page 90

FLUORIDE HIGH RANGE

Note: For wastewater or seawater samples, before performing measurements, distillation is required.

INTERFERENCES

Interferences may be caused by: Alkalinity (as CaCO

Page 91

8.25. HARDNESS, CALCIUM

SPECIFICATIONS

Range 0.00 to 2.70 mg/L (as CaCO3) Resolution 0.01 mg/L Accuracy ±0.11 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Calmagite method.

REQUIRED REAGENTS Code Description Quantity

HI93720A‑0 Calcium Hardness Reagent A 0.5 mL HI93720B‑0 Calcium Hardness Reagent B 0.5 mL HI93720C‑0 Calcium Hardness Reagent C 1 drop

REAGENT SETS

HI93720‑01 Reagents for 100 tests HI93720‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Hardness (Calcium) method using the procedure described in the Method Selection section (see page 18).

• Rinse a graduated beaker several times with unreacted

sample, before filling it to the 50 mL mark with the sample.

• Add 0.5 mL of HI93720A‑0 Calcium Hardness Reagent A

and swirl to mix.

• Add 0.5 mL of HI93720B‑0 Calcium Hardness

Reagent B and swirl to mix. Use this solution to rinse 2 cuvettes before filling them up to the 10 mL mark.

# 1

# 2

HARDNESS, CALCIUM

Page 92

• Add 1 drop of HI93720C‑0 Calcium Hardness Reagent C to one cuvette (#1).

• Replace the cap and invert the cuvette several times to mix.

This is the blank.

• Place the blank (#1) into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for

measurement.

• Remove the blank (#1) and insert the second cuvette (#2)

into the instrument.

• Press Read to start the reading. The instrument displays concentration in mg/L of calcium

carbonate (CaCO

• Press  or  to access the second level functions.

# 2

# 1

HARDNESS, CALCIUM

Page 93

HARDNESS, CALCIUM

• Press the Chem Frm key to convert the results to French degrees (°f), German degrees

(°dH), and English degrees (°E).

• Press  or  to return to the measurement screen.

Note: This test will detect any calcium contamination in the beaker, measuring syringes or sample cells. To test cleanliness repeat the test multiple times until you obtain consistent results.

SAMPLE DILUTION

This meter is designed to determine low levels of hardness, typically found in water purification systems. When testing some other sources of water, it is not uncommon to come across levels of hardness that are greater than the range of this meter. This problem can be overcome through dilution. Dilutions must be performed with hardness‑free water or the readings will be erroneous. A dilution to reduce the level of hardness by a factor of one hundred is performed as follows:

• Fill a 1 mL syringe with the sample.

• Place the syringe in a 50 mL beaker, making sure that the beaker is clean and empty, and inject 0.5 mL into the beaker.

• Fill the beaker up to the 50 mL mark with hardness‑free water.

INTERFERENCES

Interference may be caused by excessive amounts of heavy metals.

Page 94

8.26. HARDNESS, MAGNESIUM

SPECIFICATIONS

Range 0.00 to 2.00 mg/L (CaCO3) Resolution 0.01 mg/L Accuracy ±0.11 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, EDTA Colorimetric method.

REQUIRED REAGENTS Code Description Quantity

HI93719A‑0 Magnesium Hardness Reagent A 0.5 mL HI93719B‑0 Magnesium Hardness Reagent B 0.5 mL HI93719C‑0 Magnesium Hardness Reagent C 1 drop HI93719D‑0 Magnesium Hardness Reagent D 1 drop

REAGENT SETS

HI93719‑01 Reagents for 100 tests HI93719‑03 Reagents for 300 tests

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Hardness (Magnesium) method using the procedure described in the Method Selection section (see page 18).

• Rinse a graduated beaker several times with unreacted sample before filling it to the 50 mL mark with the sample.

• Add 0.5 mL of HI93719A‑0 Magnesium Hardness Reagent A, then swirl to mix.

• Add 0.5 mL of HI93719B‑0 Magnesium Hardness Reagent B and swirl to mix. Use this solution to rinse 2 cuvettes.

HARDNESS, MAGNESIUM

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HARDNESS, MAGNESIUM

• Fill both cuvettes up to the 10 mL mark.

• Add 1 drop of HI93719C‑0 Magnesium Hardness Reagent C to one cuvette (#1), replace the cap and invert the cuvette several times to mix. This is the blank.

• Add 1 drop of HI93719D‑0 Magnesium Hardness Reagent D to the second cuvette (#2), replace the cap and invert the cuvette several times to mix. This is the sample.

• Place the blank (#1) into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the blank (#1), insert the sample (#2) into the instrument and close the lid.

# 1 # 2

10 mL

# 1

# 2

# 1

# 2

Page 96

HARDNESS, MAGNESIUM

• Press Read to start the reading. The instrument displays concentration in mg/L of calcium carbonate. (CaCO3).

• Press  or  to access the second level functions.

• Press the Chem Frm key to convert the results to French degrees (°f), German degrees

(°dH), and English degrees (°E).

• Press  or  to return to the measurement screen.

Note: This test will detect any magnesium contamination in the beakers, measuring syringes, or sample cells. To test cleanliness repeat the test multiple times until you obtain consistent results.

SAMPLE DILUTION

This meter is designed to determine hardness typically found in water purification systems. In order to measure samples with high hardness, follow dilution procedure explained on page 93.

INTERFERENCES

Interference may be caused by excessive amounts of heavy metals.

Page 97

8.27. HARDNESS, TOTAL LOW RANGE

SPECIFICATIONS

Range 0 to 250 mg/L (as CaCO3) Resolution 1 mg/L Accuracy ±5 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the EPA recommended method 130.1.

REQUIRED REAGENTS Code Description Quantity

HI93735IND‑0 Hardness Indicator Reagent 0.5 mL HI93735A‑LR Hardness Low Range Reagent A 9 mL HI93735B‑0 Hardness Buffer Reagent B 2 drops HI93735C‑0 Fixing Reagent 1 packet

REAGENT SETS

HI93735‑00 Reagents for 100 tests (LR, 0 to 250 mg/L) HI93735‑0 Reagents for 300 tests (LR ‑ 100 tests, MR ‑ 100 tests, HR ‑ 100 tests)

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Hardness Total LR method using the procedure described in the Method Selection section (see page 18).

• Add 0.5 mL of unreacted sample to the cuvette. Add

0.5 mL of HI93735IND‑0 Hardness Indicator Reagent.

• With the plastic dropper fill the cuvette up to the 10 mL mark with HI93735A‑LR Hardness Low Range Reagent A.

• Add two drops of HI93735B‑0 Hardness Buffer Reagent B. Replace the cap and invert 5 times to mix.

HARDNESS, TOTAL LOW RANGE

Page 98

• Place the cuvette into the holder and close the lid.

• Press the Zero key. The display will show “‑0.0‑” when the meter is zeroed and ready for measurement.

• Remove the cuvette and add the contents of one packet of HI93735C‑0 Fixing Reagent. Replace the cap and shake gently to mix 20 seconds.

• Place the cuvette into the holder and close the lid.

• Press Read to start the reading. The instrument displays concentration in mg/L of calcium

carbonate (CaCO

• Press  or  to access the second level functions.

HARDNESS, TOTAL LOW RANGE

Page 99

HARDNESS, TOTAL LOW RANGE

• Press the Chem Frm key to convert the result to French degrees (°f), English degrees (°E), and German degrees (°dH).

• Press  or  to return to the measurement screen.

INTERFERENCE

Interferences may be caused by excessive amounts of heavy metals.

Page 100

100

8.28. HARDNESS, TOTAL MEDIUM RANGE

SPECIFICATIONS

Range 200 to 500 mg/L (as CaCO3) Resolution 1 mg/L Accuracy ±7 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the EPA recommended method 130.1.

REQUIRED REAGENTS Code Description Quantity

HI93735IND‑0 Hardness Indicator Reagent 0.5 mL HI93735A‑MR Hardness Medium Reagent A 9 mL HI93735B‑0 Hardness Buffer Reagent B 2 drops HI93735C‑0 Fixing Reagent 1 packet

REAGENT SETS

HI93735‑01 Reagents for 100 tests (MR, 200 to 500 mg/L) HI93735‑0 Reagents for 300 tests (LR ‑ 100 tests, MR ‑ 100 tests, HR ‑ 100 tests)

For other accessories see page 200.

MEASUREMENT PROCEDURE

• Select the Hardness Total MR method using the procedure described in the Method Selection section (see page 18).

• Add 0.5 mL of unreacted sample to the cuvette. Add 0.5 mL of HI93735IND‑0 Hardness Indicator Reagent.

• With the plastic dropper fill the cuvette up to the 10 mL mark with HI93735A‑MR Hardness Medium Range Reagent A.

• Add two drops of HI93735B‑0 Hardness Buffer Reagent B. Replace the cap and invert 5 times to mix.

HARDNESS, TOTAL MEDIUM RANGE