INSTRUCTION MANUAL
VISIBLE
SPECTROPHOTOMETER
HI801
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument. This manual will provide you with the necessary
information for the correct use of this instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or view our worldwide
contact list for a Hanna Instruments representative near you at www.hannainst.com.
Dear
Customer,
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
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TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION .............................................................................................................................................
2. SAFETY MEASURES ...................... .....................................................................................................................................
3. SPECIFICATIONS ...............................................................................................................................................................
4. DESCRIPTION ..... ..............................................................................................................................................................
4.1. GENERAL DESCRIPTION ..............................................................................................................................................
4.2. FUNCTIONAL DESCRIPTION .........................................................................................................................................
4.3. PRINCIPLE OF OPERATION ..........................................................................................................................................
4.4. OPTICAL SYSTEM ........................................................................................................................................................
5. OPERATING MODE ................................................... ........................................................................................................
5.1. START UP ...................................................................................................................................................................
5.2. POWER AND BATTERY MANAGEMENT ...............................................................................................................................
5.3. CUVETTE ADAPTERS....................................................................................................................................................
5.4. METHODS ......................................................................................................................................................................
5.5. TIMERS ..........................................................................................................................................................................
5.6. DATA MANAGEMENT ......................................................................................................................................................
6. SETUP .................................................................................................................................................................................
6.1. METER SETUP ..............................................................................................................................................................
6.1.1. FAVORITE METHODS ...............................................................................................................................................
6.1.2. AUTOMATIC LOG....................................................................................................................................................
6.1.3. METER ID ...............................................................................................................................................................
6.1.4. SAMPLE ID ..............................................................................................................................................................
6.1.5. BEEPER ...............................................................................................................................................................
6.1.6. LCD CONTRAST .......................................................................................................................................................
6.1.7. LETTER SCROLL .......................................................................................................................................................
6.1.8. CSV FIELD SEPARATOR .............................................................................................................................................
6.1.9. DATE AND TIME SETTING...........................................................................................................................................
6.1.10. CUVETTE DETECTION ..............................................................................................................................................
6.1.11. AUTO OFF ..............................................................................................................................................................
6.1.12. FACTORY RESET ...................................................................................................................................................
6.1.13. RESET CONFIGURATION ..........................................................................................................................................
6.2. SYSTEM CHECK ..............................................................................................................................................................
6.2.1. SYSTEM INFO ..........................................................................................................................................................
6.2.2. UPGRADE ................................................................................................................................................................
6.2.3. LAMP CHECK ............................................................................................................................................................
6.2.4. WAVELENGTH CHECK ...............................................................................................................................................
6.2.5. LAMP HISTORY ........................................................................................................................................................
6.3. USB ..............................................................................................................................................................................
6.3.1. METHODS ................................................................................................................................................................
6.3.2. REPORTS ............................................................................................................................................................
6.3.3. CONNECT TO PC ....................................................................................................................................................
6.4. METHOD SETTINGS (USER METHODS ONLY) .....................................................................................................................
6.4.1. MEASUREMENT UNIT ..........................................................................................................................................
6.4.2. NUMBER OF WAVELENGTHS .....................................................................................................................................
6.4.3. WAVELENGTH SETTING ...........................................................................................................................................
6.4.4. DECIMALS .............................................................................................................................................................
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6.4.5. DILUTION FACTOR ..............................................................................................................................................
6.4.6. VIAL TYPE .........................................................................................................................................................
6.4.7. NUMBER OF TIMERS ..........................................................................................................................................
6.4.8. TIMER SETTING ..................................................................................................................................................
6.4.9. MULTI WAVELENGTH FORMULA ..........................................................................................................................
6.4.10. CALIBRATION ...................................................................................................................................................
7. METHODS ......................................................................................................................................................................
7.1. FACTORY METHODS ..................................................................................................................................................
7.2. USER METHODS .......................................................................................................................................................
7.3. FAVORITE METHODS .................................................................................................................................................
7.4. CREATE NEW ............................................................................................................................................................
7.4.1. METHOD NAME ..................................................................................................................................................
8. WARNING AND ERROR MESSAGES.....................................................................................................................................
8.1. WARNING MESSAGES.................................................................................................................................................
8.2. ERRORS.. .................................................................................................................................................................
9. ABBREVIATIONS ..............................................................................................................................................................
10. ACCESSORIES ...............................................................................................................................................................
11. SPARE PARTS ................................................................................................................................................................
11.1. BATTERY REPLACEMENT ..........................................................................................................................................
11.2. LAMP REPLACEMENT ...............................................................................................................................................
RECOMMENDATIONS FOR USERS .........................................................................................................................................
WARRANTY .........................................................................................................................................................................
CERTICICATION.....................................................................................................................................................................
TABLE OF CONTENTS
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1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine them carefully to make sure that no damage has occurred
during shipping. Notify your nearest Hanna Customer Service Center if damage is observed.
Each HI801 iris spectrophotometer is supplied with:
• Sample Cuvette and Cap, 22 mm (4 pcs.)
• Cuvette Adapters (3 pcs.)
• Cloth for Wiping Cuvettes
• Scissors
• USB Cable
• Lithium battery
• 15 VDC Power Adapter
• Instruction Manual
• Instrument Quality Certificate
• USB Flash Drive
Note: Save all packing material until you are sure that the instrument works correctly.
Any damaged or defective item must be returned in its original packaging with the accessories supplied.
2. SAFETY MEASURES
• The chemicals contained in the reagent kits may be hazardous if improperly handled.
• Read the Safety Data Sheet (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required, and follow instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water. If reagent contacts skin, rinse the
affected area thoroughly with water. Avoid breathing released vapors.
• Waste disposal: For proper disposal of reagent kits and reacted samples, contact a licensed waste disposal provider.
To prevent injury, death or damage to the instrument:
• Use only the power supply, battery and accessories specified in the manual.
• Do not open, disassemble or modify the battery pack or instrument.
• Do not expose the battery or instrument to excess heat.
• Before storing the instrument for an extended period of time, remove the battery pack and disconnect the power plug.
• Do not use or store the battery or instrument in dusty or humid places.
• Do not shake, drop or subject the instrument to physical shock.
• Do not leave the instrument near objects with strong magnetic fields.
To prevent fire or electrical shock:
• Ensure the power adapter is completely plugged in.
• Never handle the power adapter or battery with wet hands.
• Do not leave the battery or meter near a heat source.
• Do not insert any foreign objects in the power adapter connector or battery compartment.
• Do not recharge battery outside ambient temperature conditions (0 to 45 °C).
Note: If the meter experiences a sudden temperature change allow it to equilibrate before turning on. Condensation may have
formed on the instrument and on the internal parts.
PRELIMINARY EXAMINATION SAFETY MEASURES
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SPECIFICATIONS
Wavelength Range 340-900 nm
Wavelength Resolution 1 nm
Wavelength Accuracy ±1.5 nm
Photometric Range 0.000-3.000 Abs
Photometric Accuracy
5 mAbs at 0.000-0.500 Abs
1% at 0.500-3.000 Abs
Measurement Mode
transmittance (%)
absorbance
concentration
Sample Cell
10 mm square
50 mm rectangular
16 mm round
22 mm round
13 mm round (vial)
Wavelength Selection automatic, based on the selected method (editable for user methods only)
Light Source tungsten halogen lamp
Optical System split beam
Wavelength Calibration internal, automatic at power-on with visual feedback
Stray Light <0.1 % T at 340 nm with NaNO
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Spectral Bandwidth 5 nm
Number of Methods
up to 150 factory (85 pre-loaded)
up to 100 user
Data Points Stored 9999 measured values
Export Capability
csv file format
pdf file format
Connectivity
1x USB A (mass storage host)
1x USB B (mass storage device)
Battery Life 3000 measurements or 8 hours
Power Supply
15 VDC power adapter
10.8 VDC Li-Ion rechargeable battery
Environment
0 to 50 °C (32 to 122 °F);
0 to 95% RH
Dimensions 155 x 205 x 322 mm (6.1 x 8.0 x 12.6")
Weight 3 kg (6.6 Ibs.)
3. SPECIFICATIONS
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DESCRIPTION
4. DESCRIPTION
4.1.GENERAL DESCRIPTION
The HI801 iris visible spectrophotometer is a compact and versatile instrument with a split beam optical system. It features a visible
wavelength range from 340 to 900 nm. The meter features an internal reference system, that reduces errors caused by lamp intensity
and temperature fluctuations. The optical system has been designed to minimize stray light, improving linearity and accuracy.
The spectrophotometer is supplied with 85 factory methods. These methods are pre-programmed with all of the information
necessary to complete an analysis, including the wavelength, vial type, calibration curve and timers. Up to 100 user methods can be
created. Users can select up to 5 wavelengths and timers, cuvette type, and enter their own calibration curves (concentration only).
Calibration curves can contain up to 10 points, with a linear regression curve fit to the data. The slope, offset and R-squared (R2) are
visible for the calibration curve. Both factory and user methods are easily accessible from the main screen using the favorite methods
option.
• Supplied with 85 factory methods
• Create up to 100 user methods
• 5 cuvette types (16 mm round, 22 mm round, 13 mm vial, 10 mm square, 50 mm rectangular) with automatic detection
• Data storage for 9999 measurements with ability to auto log results
• Simplified data transfer to a PC or Mac
• Field upgradeable firmware
• Rechargeable battery
4.2. FUNCTIONAL DESCRIPTION
1) ON / OFF power button
2) Keypad
3) LCD
4) Lid
5) Protective port covers
6) Indexing mark
7) Cuvette adapter
8) Cuvette
9) Battery cover
10) USB connector for USB Flash Drive
11) USB connector for PC connection
12) Power adapter connector
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The keypad contains 8 direct keys and 2 functional keys with the following functions:
Functional keys. Press to perform the function displayed above it on the LCD.
Press to access the METHOD menu.
Press to move up in a menu to increment a value or to access the FAVORITE METHODS from the MAIN SCREEN.
Press to go back to a previous level of the menu, scroll through letter places in the method creation process or to
access the TIMER MENU on the MAIN SCREEN.
Press to move down in a menu or to decrement a set value.
Press to advance in the menu, to scroll through letter places in the method creation setup or to access the CHEMICAL
FORMULAS for factory method on the MAIN SCREEN.
Press to access the SETUP menu.
Press to save the current measurement.
Press to recall logged measurements.
KEYPAD DESCRIPTION
DESCRIPTION
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DESCRIPTION
Tag Description
Battery Indicator and Charging Status
Warning Indicator
Timer Indicator
Wavelength Indicator
Tags used to indicate the selected wavelength or timer
Vial type indicator for the selected method*
Lamp Status
Indicates the Active Menu
Indicates Navigation Level in Menus
Indicates Arrow Key is Active
Calibration Indicator
Auto Logging Enabled
PC Connection Established
Flash Drive Connection Established
LCD DESCRIPTION
*Note: For factory methods the indicated vial must be used to obtain valid measurements.
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DESCRIPTION
4.3. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter.
A spectrophotometer separates electromagnetic radiation (white light) into its component wavelengths and selectively measures the
intensity of the radiation after it passes through a sample.
The white light is passed through a prism to disperse the light into bands of color. These bands of color make up the visible light
spectrum and correlate to the wavelength.
When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the
physical-chemical characteristics of the substance according to the Beer-Lambert Law:
T= I/I
o
-log I/Io = el c d
or
A = el c d
T = transmittance
A = absorbance
Io = intensity of incident light beam
I = intensity of light beam after absorption
el= molar extinction coefficient at wavelength l
c = molar concentration of the substance
d = optical path through the substance
The concentration "c" can be calculated from the absorbance of the substance as the other factors are constant.
Photometric chemical analysis is based on specific chemical reactions between a sample and reagent to produce a light-absorbing
compound.
Wavelength (nm) Absorbed Color Transmitted Color
400 violet yellow-green
435 blue yellow
495 green purple
560 yellow blue
650 orange greenish blue
800 red bluish green
I
I
o
Incident light
beam
Transmitted light beam
(light beam after
absorption)
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DESCRIPTION
4.4. OPTICAL SYSTEM
Optical System Block Diagram
A tungsten halogen lamp is used as the light source for the entire working range of the meter (340 nm to 900 nm). The tungsten
halogen lamp produces a white light that is passed to a diffraction grating.
The diffraction grating splits the polychromatic white light into the visible color spectrum, allowing for specific wavelengths to be selected.
The light is then passed through an optical filter to reduce stray light and improve measurement accuracy.
The internal reference system uses a reference photo detector to compensate for drifts due to lamp intensity, ambient temperature and
environmental changes, providing a stable source of light.
Focusing lenses are used throughout the optical system to ensure all of the light is being collected. This allows a brighter, stronger signal
to be received.
After the light exits the cuvette a final focusing lens is used. This reduces error from cuvette imperfection and scratches eliminating the
need to index the cuvette.
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5. OPERATING MODE
5.1. START UP
When the instrument is powered on, all the LCD tags will be visible for several seconds before the auto-diagnostic tests run.
This process will take several seconds, during this time the progress will be displayed on the screen. Once these tests are completed
the main screen will be displayed.
These tests ensure that the meter is working properly. If any errors occur a warning message will be displayed.
If there are no methods installed, "No Method Loaded" message is displayed.
5.2. POWER AND BATTERY MANAGEMENT
The meter can be powered from an AC/DC power adapter or from the rechargeable battery.
To conserve power, the auto off option can be enabled in the setup menu, see page 21 for more information. If this option is
enabled, the instrument will automatically turn off after a defined period of time if no interaction has occurred.
The battery icon on the display will indicate the battery and charging status:
Battery is charging from external adapter
OPERATING MODE
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OPERATING MODE
Battery fully charged (connected to power adapter)
Battery near 0% (no external power adapter)
5.3. CUVETTE ADAPTERS
The HI801 iris spectrophotometer is designed to work with five different cuvettes:
• 22 mm round
• 16 mm round
• 13 mm vial
• 10 mm square
• 50 mm rectangular
The meter is supplied with three cuvette adapters:
adapter for 16 mm cuvette
adapter for 10 mm square cuvette
adapter for 13 mm vial
Note: The 22 mm round and 50 mm rectangular cuvettes do not require adapters. The cuvettes can be directly inserted into
the meter.
To prepare the meter for the use of adapters:
1. Open the meter's lid.
2. Select the adapter according to the cuvette type required for the selected method.
3. Orient the adapter so that indexing mark is aligned with the indexing mark located inside the meter.
4. Using light pressure, push the adapter down until it reaches the bottom of the meter's holder.
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OPERATING MODE
5. The meter is ready for use. Always utilize the selected adapter for both "Zero" and "Read" measurements as specified in the
method instructions.
WARNING: Improper use of the vial adapters could cause irreversible damage to the meter. Always use the flowing
precautions when using vial adapters.
•Never use excessive force to insert the adapter. You should be able to insert the vial with light pressure. If the vial
is not reaching the bottom, if there is large resistance, or if you are receiving a “light low” error during the “Zero”
operation, re-check that the indexing marks are aligned on the adapter and meter.
•Never insert hot vials/samples into the vial adapter. Samples should be near room temperature before inserting
into the meter/adapter.
•Do not attempt to close the sample cover while using the 13 mm vials or adapter. It is normal for the vials/
adapter to prevent the cover from closing completely.
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OPERATING MODE
5.4. METHODS
The HI801 iris spectrophotometer can run two different types of methods; factory methods and user methods.
Factory methods were developed by Hanna Instruments. These methods are pre-programmed with all of the information needed
to run an analysis. These methods are calibrated for the selected wavelength, vial type and reagent set, see page 37 for more
information.
User methods are developed by the user. These methods can be customized based on the analysis. Options include multiple
wavelengths, vial type, reaction timers, calibration curves, etc. For additional information for creating new user methods, see
page 39. For additional information about modifying a user method, see page 28.
Factory and User Methods can be tagged as Favorite Methods, if enabled, see page 17 for more information. Favorite Methods are
easily accessible from the main screen by pressing the key.
5.5. TIMERS
Each method requires a different measurement procedure.
If a timer or timers is used during the measurement procedure the key will be visible on the main screen with the TIMER tag
above it.
Press the key to access the timer menu. Press the START key to start Timer 1, the display will show the countdown. To stop
and reset the timer, press the STOP key.
If the method requires more than one timer. Press the key to access the timer menu and press the key to select Timer 2 to
Timer 5.
When the timer has expired press the ZERO or READ key to continue. If the timer beeper is enabled a long beep will be heard when
the timer reached "00:00", see page 18 for more information.
Note: A zero measurement must be done before a read measurement. Follow the instructions in the method procedure for
preparation of the zero cuvette.
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