Hanna Instruments HI 731340 User Manual

HINTS FOR ACCURATE MEASUREMENTS

Instruction Guide

1. Use the pipette in a vertical position whenever possible.

2. Operate slowly and smoothly. Aspiration and dispensing should
been carried-out with the same speed.

3. The tip should be immersed in the sample liquid the minimum
necessary (2-4 mm). Greater depths may cause inaccurate
measures.

4. Change the tip whenever a different kind of sample is to be
aspired or when droplets remain on the inside wall of the tip
from the previous pipetting operation.

5. Work always with samples at room temperature.

PIPETTE MAINTENANCE

1. Liquid should never enter the pipette shaft. To avoid this:

• never use pipette without a tip

• do not invert pipette upside down or lay it flat when there

is a liquid in the tip

• press and release the push button slowly and smoothly.

2. When pipetting concentrated acids or alkalis or corrosive
solutions, pay particular attention to avoid contact of the
liquid with the pipette shaft, or it could be seriously damaged.
Extensive contact with corrosive fumes may result in premature
ageing of internal components of the pipette.

3. After use, store the pipette in its case or in a clean safe
place.

ACCESSORIES

HI 731350 tips for 200 µL automatic pipette (25 pcs)
HI 731351 tips for 1000 µL automatic pipette (25 pcs)
HI 731352 tips for 2000 µL automatic pipette (4 pcs)

Fixed-Volume Pipette

HI 731340
HI 731341
HI 731342

http://www.hannainst.com

Dear Customer,
Thank you for choosing a Hanna Instruments product.

This instruction guide will provide you with the necessary
information for the correct use of the pipette. Please read it
carefully before using the pipette. If you need additional technical
information, do not hesitate to e-mail us at [e-mail].

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that no
damage has occurred during shipment. If there is any
damage, notify your Dealer.

Each pipette is supplied complete with:

• Two tips

• Instruction Guide

• Rigid carrying case

Note: Keep all packing material until you did verify that the

pipette works correctly. Any defective item must be
returned in its original packing.

GENERAL DESCRIPTION

Hanna Instruments reserves the right to modify the design, construction
and appearance of its products without advance notice.

Hanna Instruments Srl
V.le delle Industrie 12/A

http://www.hannainst.com

35010 Villafranca (Padova)
ITALY

HI 731340/1/2 is an air-displacement volumetric pipette
designed to measure and transfer fixed volumes of liquids
that needs to be precisely dosed.

The liquid is aspirated into deposable tips. The use of
disposable tips eliminate cross contamination of the liquid
samples.

To discard used tips, the pipette is equipped with a built-in
tip ejector to protect the user from contaminated tips.

ISTMICPIPR1 05/03

All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner, Hanna Instruments
Inc., Woonsocket, Rhode Island, 02895 , USA.

Pushbutton

second stop

first stop

Body

Shaft

Description:

Operation:

Tip ejector button
Pipette volume:

200, 1000 or 2000 µL

Tip ejector arm

Disposable tip

OPERATION

1. Mounting a tip:
Place a new tip on the pipette by pressing and gently rotating

the shaft into the tip. The tip will fit firmly the shaft.
Note: Never use the pipette without a tip.

2. Aspiration:

• Press the pushbutton to the first stop (fig. A). Holding the

pipette vertically, immerse the tip into the sample liquid to
the depth of 2-4 mm.

• Release the pushbutton slowly and smoothly (3-6 seconds).

Pause (1-2 seconds) to allow the full volume of liquid to fill up
the tip (longer for high density or viscous liquids) (fig. B).

• Withdraw the pipette out of the liquid. If any liquid remains

on the outside of the tip, wipe it away carefully with a lint-free
cloth (take care not to touch the tip orifice).

3. Dispensing:

• Holding the pipette vertically, position the tip end against

the sidewall of the receiving cuvet at a 40-45° angle. Expel
the liquid by pressing slowly the pushbutton; pass by the first
stop to the second stop (bottom of the stroke length). Wait 1­3 seconds to empty completely the tip (longer for viscous
liquids) (fig. C).

• Still holding the pushbutton depressed, withdraw the pipette

sliding it along the wall of the vessel. Then release the
pushbutton.

• Discard the tip to waste container by pressing the tip ejector

button (fig. D).

• The pipette is ready for the next pipetting cycle. To prevent

cross contamination use a new tip whenever a different kind
of sample is to be aspirated.

• The pipette tip may be used several times, rinse the tip

immediately after use. A dirty tip may cause cross contamination
or wrong sample volumes may be transferred.

ADBC

Technical data:

Pipette code Tip code Volume Accuracy Precision

(µL) (%) (%)

HI 731340 HI 731350 200 ±1.5 ≤0.8
HI 731341 HI 731351 1000 ±1.2 ≤0.5
HI 731342 HI 731352 2000 ±1.0 ≤0.3