Hach-Lange LUMISTOX 300 User Manual
LUMIStox 300 Operating Manual
Dr. Bruno Lange GmbH
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LUMIStox 300
Manual
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January 1999
Version 3.02 and above
BDA 356
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LUMIStox 300 Operating Manual
Dr. Bruno Lange GmbH
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Dear Customer,
We are delighted that you have chosen the LUMIStox 300 measuring instrument by Dr.
Bruno Lange GmbH, and wish to thank you for the confidence you have shown in us.
This measuring instrument embodies the technology of the future. LUMIStox 300 is the
product of many years of experience in the field of analysis with the luminescent
bacteria test.
LUMIStox 300 was developed so that it could be operated simply and without any need
for considerable previous study. Nevertheless, you should read this operating manual
carefully before you use the instrument. This will help you to avoid operating errors and
misunderstandings.
If you have any questions that are not answered in this operating manual, please ring
our technical advice service in Düsseldorf:
Dr. Elmar Grabert Tel.: ++49/(0)211-5288-241
Margret Link Tel.: ++49/(0)211-5288-126
Fax: ++49/(0)211-5288-175
Your
Dr. Bruno Lange GmbH
D-40549 Düsseldorf, Germany
PS: Which language do you prefer?
The language presetting of LUMIStox 300 is German. To change the language please
follow the next steps:
1. Switch on the instrument as decribed in the manual.
2. Follow the description on page 7 to get the menu LU Opt Ende à
3. Press the key under <Opt>. You will get Farb 100 ß à
4. Press the key under <à> four times until you will reach Spra ß à
5. Press the key under <Spra> to get the language selection menu
D GB I E
6. Select your language by pressing the key under
D: German; GB: English; I: Italian; E: Spanish
7. After changing you will reach the main menu by pressing the key under <ß>.
We reserve the right to make any changes in the interests of improving the instrument.
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January 1999
with effect from Version 3.02
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LUMIStox 300 Operating Manual
Dr. Bruno Lange GmbH
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CONTENTS
1 The LUMIStox 300 measuring instrument 3
1.1 Concept 3
1.2 Starting up 4
1.3 Additional information 5
1.4 Procedure in short 6
2 Operation of the LUMIStox 300 measuring instrument 7
2.1 Switching on 7
2.2 Explanation of the LUMIStox 300 menu guidance 8
2.3 Initial settings for luminescent bacteria tests for determining GL and EC 9
2.4 Screening 11
2.5 Determining the GL value 13
2.6 Determining the EC value 15
2.7 Determining chronic toxicity with LUMIS·24·tox 17
2.8 The LUMIStox luminescent bacteria test (LCK484) 20
2.9 The TOX cuvette test (LCK488) 21
2.10 Determining optical density (OD) 23
2.11 Determining relative light units (LU) 24
3 Options 25
3.1 Colour correction 26
3.2 Standardization of luminescence measurements relative to 100 27
3.3 Self-test 28
3.4 Setting the serial interface (V24) 29
3.5 Setting the print options 30
3.6 Setting the date and time 31
3.7 Setting the temperature of the measuring shaft 32
3.8 Setting the screen contrast 32
4 Appendix 33
4.1 Sample preparation for the luminescent bacteria test 33
4.2 Determining EC and GL values 34
4.2.1 Preparing a dilution series 34
4.2.2 Practical advice: The geometrical dilution series 35
4.2.3 Practical advice: Dilution series in conformity with DIN 38412 L34/341 36
4.3 Calculation of the GL value in conformity with DIN 34412/L34/341 37
4.4 Calculating the EC value 38
5 Printout 39
6 Error messages 40
7 Technical data 41
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LUMIStox 300 Operating Manual
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1 The LUMIStox 300 measuring instrument
1.1 Concept
The LUMIStox 300 is a measuring instrument that has been developed as a measuring
and evaluation unit for the luminescent bacteria test. In combination with the
LUMIStherm incubation block it conforms to the technical requirements of DIN 38412
L34 and L341 and the international standard ISO DIS 11348.
In addition the Dr. Lange luminescent bacteria tests for operational analysis LUMISmini
(LCK484) and TOX cuvette test (LCK488), as well as the luminescent bacteria test for
chronic toxicity, LUMIS·24·tox (LCK486), can be evaluated with this instrument.
The LUMIStox 300 combines computer technology with the technology of measuring
instruments (in this case a luminometer). Just like a computer, the LUMIStox 300 has
its own operating system. This is externally apparent from the built-in diskette drive. A
diskette, on which all the data and programs needed to operate the LUMIStox 300 are
stored, starts ("boots") the instrument just like a computer when it is switched on. Future
program extensions or updates can therefore simply be read into the instrument from a
new diskette.
The LUMIStox 300 has a built-in photometer function and an automatic measuring and
evaluation routine, which enable it to recognize colour effects in the luminescent
bacteria test and to take account of these in the test result. The photometer function
also allows the colour effect to be estimated in advance, and can be used to determine
the extinction (as OD - optical density) of bacteria suspensions for the purpose of
assessing growth inhibition in the luminescent bacteria test for chronic toxicity,
LUMIS·24·tox (LCK486).
The LUMIStox 300 has an automatic reference control system, with which it checks the
functioning of the whole measuring path before each luminescent bacteria test is
carried out.
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1.2 Starting up
If necessary, connect the LUMIStox 300 to a printer through the 35-pin parallel (par)
interface, or with a computer through the 9-pin serial (ser) interface. Connect all the
devices to the mains power supply. Make sure that the LUMIStox 300 system diskette
is in the diskette drive.
Switch on the peripheral devices first, then the LUMIStox 300. In the same way as a
PC, the LUMIStox 300 starts up by displaying a series of messages on the screen.
Eventually <Temp: XY.Z °C Bitte warten> appears.
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Layout of the LUMIStox 300
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1 Mains power connection 6 25-pin parallel interface (par)
2 Main switch with cutout 7 3.5" diskette drive
3 Liquid crystal display 8 Nameplate
4 Numeric keyboard 9 Cuvette shaft
5 9-pin serial interface (ser) F1-F4 Function keys
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min
1.3 Additional information
You should take account of the following advice to ensure that your LUMIStox 300
works correctly and reliably.
The system diskette must be inserted into the drive before
the instrument is switched on! Whenever the instrument
is moved, the diskette must first be removed from the
drive.
The LUMIStox 300 measuring instrument should not be
operated in an ambient temperature below 16°C or above 29°C,
otherwise problems may occur with the cooling of the
measuring shaft.
Do not operate the instrument in direct sunlight!
Soiling impairs the functioning of the cuvette lowering system. For
this reason, do not pipette reagents into measuring cuvettes in
the measuring shaft. The measuring shaft should also be closed
in the <exit > mode when the measurements have been
completed. When the LUMIStox 300 is in use, the measuring shaft
is automatically closed after a 10-minute idle period. It can be
opened again by pressing any key.
Before any measurements the LUMIStox 300 should have been
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switched on for the least 30 minutes so that the photomultiplier
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and the cooled components are ready for operation.
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1.4 Procedure in short
1. Switch on printer and PC, if connected.
2. Insert system diskette in LUMIStox 300 diskette drive.
3. Switch on LUMIStox 300.
4. Switch on LUMIStherm incubation block.
5. Wait 30 minutes to allow instrument to thermostate.
6. During the thermostating period:
• Select the luminescent bacteria test and reactivate
the bacteria as described in the test instructions.
• Add 2 % sodium chloride to the sample and check the pH.
• If necessary, prepare a sample dilution series.
7. Switch on automatic colour correction if necessary.
1.
LCK 484
1. Pro b e aufs alze n
2. Le uchtba kterie n rea ktivie ren
0
Minute n
3. Kont roll- und Probenlös ung(e n)
in Meß k üvett en geb en
2% NaCl Pr obe
Kontrolle Probe
4. Leu c htbak terie n in Meß küve tten ge ben
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Minuten
5. Mes sen u n d Ergeb nis ab lesen
30
Minuten
Leuchtba kterien
Kontrolle Probe
8. Set the LUMIStox 300 to the required evaluation mode (GL or EC value) or to the
test that is to be carried out (e.g. LCK488).
9. Key the requested initial settings into the LUMIStox 300.
10. Carry out the test in line with the working procedure
and the LUMIStox 300 menu guidance.
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11. Read the result.
12. Close the measuring compartment.
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2 Operation of the LUMIStox 300 measuring instrument
2.1 Switching on
After the LUMIStox 300 has been switched on, the following displays are shown:
LUMIStox 300
Version 2,21
Version
number
LUMIStox 300
13:40 14.02.97
temp.: 14.7°C
please wait
scrn EC GL test OD chroni LU opt exit
LSoft
abbreviations in the main menus have the following meanings:
Scrn = Screening
EC = Determination of EC values
GL = Determination of GL in conformity with DIN 38412 L34/341
test = LUMISmini luminescent bacteria test and TOX cuvette test
OD = Determination of extinction at 485 nm
Chroni = Determination of chronic toxicity
Date and time
Instrument
being brought
to required
temperature
Main menu
levels
The
LU = Determination of relative luminescence
opt = Setting the instrument parameters
exit = Closing the measurement compartment
Lsoft = Working with the LUMISsoft Calculation software
→ = Go forward in this menu
← = Go back to previous menu
The operator action that must or can be carried out in connection with a given display is
indicated with the function keys under the display field. In this context
n = Press this function key
The reference check on the measurement path is carried out automatically after a
measurement program is selected.
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2.2 Explanation of the LUMIStox 300 menu guidance
Depending on the program and the requirements, the LUMIStox 300 offers the user a
variety of options for selecting and modifying programs and settings, which are listed
and whose operation is described here.
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Entries are made with the function keys F1 to F4 below the
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screen or with the numeric keyboard, for example:
C
The function key located below a required program is
s c r n E C G L
d i l u t i o n s t e p s
? 4 o k
c o l .c o r r . : v a r
p e r m o f f v a r
b i t / s = 9 6 0 0
+ - o k
d a t a b it s = 8
7 8 o k
pressed to load the program.
Numeric values can be changed with the help of the
numeric keyboard or confirmed by pressing the function
key located below <ok>.
The setting shown at the top right can be changed by
pressing the function key located below the required new
setting. The new setting must be confirmed by pressing the
function key located below <←>.
The setting at the top right can be changed by pressing the
function key located below <+> or <->. The new setting
must be confirmed by pressing the function key located
below <ok>.
r e f e r e n c e c h e c k
p l e a s e w a i t
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The setting can be changed by pressing the function key
located below the required setting. The new setting must be
confirmed by pressing the key located below <ok>.
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2.3 Initial settings for luminescent bacteria tests for determining GL
and EC
Initial settings have to be entered for the luminescent bacteria test to enable the GL or
EC value to be calculated. The necessary settings are requested by the LUMIStox 300
after the evaluation mode <GL> or <EC> has been selected. Their significance and the
method of entering them are explained below.
<N> indicates that all the current settings are to be adopted.
settings
change ? J N
dilution steps
? 4 ok
pre dilution: no
yes no ok
The LUMIStox 300 then switches to the start of the
measurement procedure. If <Y> is chosen, the following
settings are requested in sequence.
The length of the dilution series can be entered with the
numeric keyboard (at least 3 and no more than 9 dilution
stages) or the current setting can be confirmed with <ok>.
Preliminary dilution of a toxic sample is rejected with <no >.
The current setting is adopted with <ok>.
After confirmation with <yes >, the required preliminary dilution
Only preliminary dilutions from the dilution series referred to in the DIN standard
are therefore allowed for GL determinations.
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can be entered with the numeric keyboard
(not shown). It is taken into account in the EC or GL value.
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type of dil.:DIN
DIN 2 ok
unit: mg/l
mg/l g/l % ok
ini.conc. in mg/l
? 100.0 ok
<DIN> confirms the dilution series referred to in the DIN
standard, while <2> confirms the simple geometrical
series. <ok> indicates that the current setting should be
adopted.
The unit for the sample concentration is specified here.
For environmental samples of unknown composition, e.g.
waste water, <%> should be confirmed.
In this case the current setting can be adopted with <ok>
or a new value, corresponding to the concentration of the
original sample without preliminary dilution, can be
entered with the numeric keyboard. For example, for the
time of incub. 1
? 15 ok
time of incub. 2
? 30 ok
time of incub. 3
? 0 ok
analysis of waste water you can enter 100 here.
In this case <ok> indicates that the current setting should
be adopted. Otherwise a new value can be entered with
the numeric keyboard, e.g. 5, 15 and 30 minutes. If <0> is
entered as the incubation time 2, incubation time 3 is not
requested. Take account of the speed with which you
work and, depending on the length of the dilution series,
do not set incubation time 1 to too small a value.
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2.4 Screening
Screening is the simpliest way of determining the toxicity of a sample. The result is
expressed as the percentage inhibition of the luminescence in the test solutions relative
to a control solution. With a LUMIStherm, double determinations of 9 samples or single
determinations of 14 samples can be carried out in one sequence.
Work steps:
1. Prepare sample(s).
2. Pipette 1.5 ml (for double determinations) or 1 ml (for single determinations) into
each measuring cuvette and bring to the correct temperature in the LUMIStherm.
3. Bring 1.5 ml or 1 ml of the control solution (2% NaCl solution) to the correct
temperature in position A1 of the LUMIStherm.
4. Reactivate the preserved bacteria by following the instructions in the package
circular.
5. Pipette 0.5 ml bacteria suspension into measuring cuvettes.
6. Quickly add 0.5 ml control solution or sample to the bacteria suspensions.
7. Wait for the incubation period, e.g. 5, 15 or 30 minutes, to elapse.
8. Select evaluation mode <scrn> on the LUMIStox 300.
9. Measure the control and test solutions and record or print the results.
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Sequence of operations on the measuring instrument
scrn EC GL
reference check
please wait
ref. cuvette
measure
ref. measured
measure
Select the evaluation mode <scrn>
Measure the control solution,
e.g. from position B1
Measure first test solution,
e.g. from position B2
Record or print the result;
25.3 % inhib.
measure
measure next test solution, etc.
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