GE UV-1 User Manual
Monitor UV-1
59-7797-01
Edition AG
User Manual
Important user information
Reading this entire manual is
recommended for full
understanding and use of
this product.
●
The exclamation mark within an equilateral
triangle is intended to alert the user to the
presence of important operating and
maintenance instructions in the literature
accompanying the instrument.
Should You have any comments on this manual,
we will be pleased to receive them at:
GE Healthcare Bio-Sciences AB
S-75182 Uppsala
Sweden
GE Healthcare Bio-Sciences AB reserves the right to
changes in the specifications without prior
make
notice.
Warranty and Liability
GE Healthcare Bio-Sciences AB guarantees that the
product delivered has been thoroughly tested to
ensure that it meets its published specifications.
The warranty included in the conditions of
delivery is valid only if the product has been
installed and used according to the instructions
supplied by GE Healthcare Bio-Sciences AB.
GE Healthcare Bio-Sciences AB shall in no event be liable
for incidental or consequential damages,
including without limitation, lost profits, loss of
income, loss of business opportunities, loss of
use and other related exposures, however
caused, arising from the faulty and incorrect use
of the product.
Trade marks
FPLC
GE Healthcare Bio-Sciences AB.
In view of the risk of trade mark
degeneration, it is respectfully suggested that
authours wishing to use this designation refer to
its trade mark status at least once in each article.
®
is the exclusive trade mark of
Copyright© 1995 GE Healthcare
Bio-Sciences AB
All rights reserved. No part of this publication
may be reproduced, stored in a retrieval system
or transmitted in any form by any means,
without permission in written form from the
company.
Contents
1. Introduction........................................................................................4
2. General Description..........................................................................5
2.1Basic principle.............................................................................5
2.2Optical unit.................................................................................7
2.3Control unit.................................................................................9
3. Installation........................................................................................11
3.1Site requirements......................................................................11
3.2Unpacking.................................................................................11
3.3Electrical connections..............................................................11
3.4Installation of the filter............................................................12
3.5Installation of the flow cell.....................................................13
3.6Connecting the optical unit....................................................14
3.7Connecting a recorder.............................................................14
4. Operation..........................................................................................15
4.1Choice of wavelength..............................................................15
4.2Choice of AU or transmission (%T).......................................15
4.3Conversion table T% to AU and OD.....................................16
4.4Start-up......................................................................................16
4.5Stabilization time......................................................................16
4.6Basic operating procedure - AU.............................................17
4.7Basic operating procedure - Transmission (%T)..................17
4.8Shut down.................................................................................17
5. Maintenance.....................................................................................18
5.1General precautions.................................................................18
5.2Cleaning the flow cell..............................................................18
5.3Changing the flow cell............................................................19
5.4Interference filters....................................................................20
5.5Other optical surfaces..............................................................20
5.6Instrument housing.................................................................20
5.7Lamp and optical system test.................................................20
5.8Changing the mercury lamp...................................................21
5.9Mercury lamp - maximum light adjustment........................22
6. Trouble-shooting..............................................................................23
7. Technical Specifications..................................................................24
8. Accessories and Spare Parts...........................................................25
3
1. Introduction
1. Introduction
The UV-1 is a fixed wavelength UV monitor, consisting of a control
unit and an optical unit that can be positioned up to 10 meters apart.
A mercury lamp is the stable light source, furthermore a built-in
reference cell eliminates baseline drift. The output signal can be recorded in either AU or %T. Sensitivity range between 0.01 and 2 AUFS,
or (0-100%T).
The UV-1 offers a range of three detection wavelengths:
254, 280 and 405 nm. In addition, five flow cells are available for
different applications in Standard Chromatography, FPLC and
Industrial applications.
4
2. General Description
2. General Description
2.1 Basic principle
GE Healthcare LKB Monitor UV-1 consists of an optical unit
containing the flow cell, lamp, filter assemblies and preamplifiers, and a
control unit containing the signal processing circuits. The two units are
connected via a multi-core cable hardwired from the control unit to
the optical unit. Connection to the recorder and the mains supply is
made via the control unit.
The single path Monitor UV-1 is a dual beam instrument with a
sample cell and a reference cell. Optical path lengths are 10 mm,
3 mm, or 2 mm depending upon the flow cell chosen.
Note:
The sample cell and reference cell are angled to receive light from the
same point in the lamp (Fig. 1) thus assuring a stable base-line by
negating the effects of variations in lamp intensity.
The lamp is powered by a stabilized DC to AC converter operating at
20 kHz to maximize its efficiency. Lamp output is independent of
variations in the line voltage.
Light from the lamp passes through an aperture (254 nm, 405 nm) or
a fluorescence converter (280 nm) and through the reference cell and
sample cell. The light output from each cell passes through an
interference filter and falls onto a solid state photo-detector whose
output is a linear function of the light intensity (Fig. 1 and Fig. 2).
Flow cell S-2 has only the sample cell.
Fig. 1. Optical path.
5
2. General Description
Fig. 2 Block diagram
The photocurrent from each detector is amplified in a pre-amplifier
before passing to the signal processing circuitry in the control unit.
If transmission is to be monitored, the signal passes directly to the
low pass filter. If AU is to be monitored, the reference cell light
intensity (IR) is compared with the sample cell intensity (IS) to form
log (IR/IS) in a logarithmic circuit before passing to the low-pass filter.
The signal finally passes to the range selector before being presented
to the output terminal.
6
2. General Description
2.2 Optical unit
Front panel controls
12
5
4
3
Fig. 3.
Optical unit. Front panel.
No. Item Description
1 Cell holder The complete cell holder is removed by turning the
locking (Fig. 4:6) knob on the rear panel
2 Sample inlet Inlet for sample flow
3 Sample outlet Outlet for sample flow
4 Reference inlet Inlet for flowing reference liquid. The reference cell
may be operated dry, with static reference liquid
5 Reference outlet Outlet for flowing reference liquid
7
2. General Description
Rear panel controls
6
11
9 Guide pin Screw 7 10 8
Fig. 4.
No. Item Description
6 Locking knob The cell holder is in locked position when the locking
7 Filter inlet Filters, converters or apertures are inserted in the
8 Converter or the arrowhead on the end of the filter or converter
9 Support rod Enables the optical unit to be mounted on laboratory
10 Support rod Alternative position for support rod
11 Multi-core cable Connects the optical unit to the control unit via
Optical unit. Rear panel.
knob is turned fully in the direction of the arrow
positions indicated. When inserting them, align
Aperturewith the arrowhead next to the appropriate
opening. Filters, converters and apertures must be
pressed fully
scaffolding. The rod may be secured in either of two
positions by a screw and a guide-pin
an 11-pin plug with a snap-lock. For disconnection
squeeze the ribbed sides of the plug firmly and pull
Warning: The optical unit contains a UV-source which is exposed
if the cover is removed.
8
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