GE GeneQuant pro User Guide

GeneQuant pro

GE imagination at work

	GeneQuant pro /
	(Part number)		80-2114-98/99, 80-2115-04/05
	(Serial number)		79000

Biochrom :

73/23/EEC & 89/336/EEC

EN 61010-1: 2001

EN 61326:1998

:	2002 10 23

David Parr

Biochrom

(Postal address):	(Telephone):	(Telefax):
Biochrom Ltd	[phone]	[phone]
22 Cambridge Science Park

Milton Road

Cambridge CB4 0FJ

England

E-mail:[e-mail]

Website:http://www.biochrom.co.uk(Registered in England No) 974213

(Registered Office) 22 Cambridge Science Park, Milton Road, Cambridge CB4 4FJ, England

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DNA RNA ……………………………………………………………7

*NAQ ……………………………………………………………………7

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*595 546 562nm ………………………………………………………9

*280nm …………………………………………………………………10

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DNA RNA …………………………………………………………………12

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(Tm) ……………………………………………………………………………15

595nm …………………………………………………………………………16

280nm …………………………………………………………………………17

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PC …………………………………………………………………………………19

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10 40

31 80 40 50

•4

•5cm

•100~240V

• 出现“Instrument Ready”

2.(1= ,0 = )

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GeneQuant pro RNA/DNA 230 260 280 320 546 562 595 600nm

•PCR (DNA RNA )

( µg/ml , ng/µl, µg/µl, pmole/µl ) 260nm230 280nm 260/230 260/280 320nm

UV

•PCR Tm(Breslauer et al , Proc. Natl. Acad. Sci. USA, 83, 3746, 1986)

•595nm Bradford 546nm 562nm BCA

•OD600

(electronic project archiving)PC

standard Centronics parallel printer

(Calibration check filter set)GLP PC

将产品使用成本降到最低。

128×64 28

( “Instrument Ready” )

•(set up) Bradford

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• 至前的轴线方向。

•“enter”12

Utility Keys

	Setup		Set up
	cal check
	select
	print		, PC .
	base Seq.
	Tm		Tm Abs260 Tm Tm
	stop		“ escape ”
	setref
	enter
	(Reading keys)
				230 260 280 320 595 600nm
		abs
				230 260 280 320nm DNA 260/280
	DNA
				260/230
				230 260 280 320nm RNA 260/280
	RNA
				260/230
				230 260 280 320nm
	oligo
				260/280 260/230
				595 546 562nm
	prot. 595

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prot. 280

260 280 320nm

cell cult. 600nm OD

DNA RNA

(NAQ)

• 260nm 10mm 1.0 DNA RNA50 40µg/ml 33µg/ml

= ×Abs 260

•DNA RNA 50 40 33 10mm “Set up”

•µg/ml ng/µ1 µg/µ1 pmol/µl pmol

“Set up” ( PCR pmo1/µ1 )

1µg/ml = 1ng/µl = 0.001µg/µ1

pmol/µl =

µg/ml×1000

pmol = , µg/ml 315

•DNA (MW)

MW(g/mole) = (dA×312.2 + dc× 288.2 + dG× 328.2 + dT× 303.2) + ×

RNA (dU×298.2) (dT×303.2) (MW)5’ 3’

17+2 ×

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61+

(g/mole) (Na, Sodium 23.0) (K, Potassium 39.1) (TEA Triethylammonium 102.2)

•260/280DNA RNA≥1.8 ≥2.0

•260nm 280nm( ) 280nm260/280 260nm

•260/280 260nm 280nm>0.05

•230nm 230nmTris EDTA RNA260/230 >2.0 (guanidinium thiocyanate) RNA 230~260nmRNA

•260/280 260/230 10mm“Set up”

•260 280nm320nm “Set up”

•320nm 260 280nm 320nm

=(A260-A320)×

Abs =(A260-A320)/(A280-320)

Abs =(A260-A320)/(A280-320)

•NO

• 造成的改变。

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