Fujifilm QuickGene-610L User Guide

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Introduction

Thank you for using the Nucleic Acid Isolation System QuickGene-610L (hereafter, called "QuickGene610L").Easy operation of this device enables extraction of DNA and RNA. This manual contains important information regarding the safe and proper use of the QuickGene-610L functions. Read this manual carefully before use. Keep this manual after reading, for reference whenever necessary.

About Infectious Materials

When handling or disposing of infectious materials, follow the laboratory guideline or the law regarding infectious wastes to perform the incineration, fusion, sterilization, and/or disinfection. When you use a third party to dispose it, outsource it to a licensed operator for industrial waste subject to special control, and give them the management table (manifest) for industrial waste subject to special control at the same time.

■ Exclusion clauses

• The contents of this manual may be updated without notice.

• Fuji Photo Film Co., Ltd. assumes no liability for damages to third party copyrights or other rights arising from the use of any information in this manual.

• Fuji Photo Film Co., Ltd. assumes no liability for device failures or damages due to installation, relocation, remodeling, maintenance, and repairs performed by parties other than Fuji Photo Film Co., Ltd. or agencies specified by Fuji Photo Film Co., Ltd.

• Fuji Photo Film Co., Ltd. assumes no liability for failures or damages of our products due to third-party products other than products delivered by Fuji Photo Film Co., Ltd.

• Fuji Photo Film Co., Ltd. assumes no liability for device failures or damages due to remodeling, maintenance, repairs, etc. using repair parts other than genuine parts specified by Fuji Photo Film Co., Ltd.

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• Fuji Photo Film Co., Ltd. assumes no liability for device failures or damages due to failure to observe the precautions and operation procedures described in this manual.

• Fuji Photo Film Co., Ltd. assumes no liability for device failures or damages due to using the device in an environment that does not meet the operational requirements (power, installation environment, etc.) described in this manual.

• Fuji Photo Film Co., Ltd. assumes no liability for device failures or damages due to natural disasters such as fire, earthquakes, floods, or lightning.

• No part of the contents of this manual may be reproduced without permission from Fuji Photo Film Co., Ltd.

■ Trademarks

Company names and product names are trademarks or registered trademarks of their respective owners.

QuickGene-610L User’s Guide Rev. 1.0 i

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Notations

This manual contains important information regarding the safe use of the QuickGene-610L. Read this information carefully before using the QuickGene-610L, and start operation. The meanings of safety precaution marks are as follows:

WARNING:

BIOHAZARD

CAUTION:

Important: "Important" shows the important notes for usage, as well as prohibited actions.

Note: "Note" indicates the notes, procedures that should be obeyed, and supplementary information for use.

"WARNING" indicates a dangerous condition that may lead to death or serious injury.

"BIOHAZARD" indicates exposure to a biological agent or condition that may be infectious or harmful.

"CAUTION" indicates the possibility of danger that may lead to minor or moderate personal injury. "CAUTION" may also indicate an accident of property damage.

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QuickGene-610L User’s Guide Rev. 1.0 iii

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Introduction

Merci d’avoir choisi le Système d’isolation de l’acide nucléique QuickGene-610L (appelé ci-après "QuickGene-610L"). Le fonctionnement simple de cet appareil permet l’extraction d’ADN et d’ARN. Ce manuel contient des informations importantes concernant l’utilisation en toute sécurité du QuickGene610L. Lisez attentivement ce manuel avant toute utilisation. Après lecture, conservez ce manuel pour pouvoir vous y référer ultérieurement si nécessaire.

A propos des produits infectieux

Lorsque vous manipulez ou mettez au rebut des produits infectieux, veillez à respecter les directives du laboratoire ou la loi les concernant pour en effectuer l’incinération, la fusion, la stérilisation et ou la désinfection. Lorsque qu’un tiers est chargé de le faire, veillez à le confier à une entreprise habilitée à traiter des déchets industriels sujets à contrôle spécifique et fournissezleur en même temps le tableau de gestion (le manifeste) des déchets industriels sujets à contrôle spécifique.

■ Clauses d’exclusion

• Le contenu de ce manuel peut être mis à jour sans préavis.

•

Fuji Photo Film Co., Ltd. ne peut être tenu pour responsable de dommages aux copyrights de tiers ou d’autres droits émanant de l’utilisation d’une information quelconque contenue dans ce manuel.

• Fuji Photo Film Co., Ltd. ne peut être tenu pour responsable de défaillances ou de dommages de l’appareil causés par l’installation, la relocalisation, la modification, la maintenance, et les réparations effectués par des tiers autres que Fuji Photo Film Co., Ltd. ou des agences agréées par Fuji Photo Film Co., Ltd.

• Fuji Photo Film Co., Ltd. ne peut être tenu pour responsable des défaillances ou des dommages de ses produits dus à des produits tiers autres que ceux fournis par by Fuji Photo Film Co., Ltd.

• Fuji Photo Film Co., Ltd. ne peut être tenu pour responsable des défaillances ou des dommages de l’appareil dus à la modification, la maintenance, les réparations etc. utilisant des pièces de rechanges qui ne sont pas des pièces d'origine spécifiées par Fuji Photo Film Co., Ltd.

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• Fuji Photo Film Co., Ltd. ne peut être tenu pour responsable des défaillances ou des dommages de l’appareil dus à la non observation des procédures de fonctionnement et des précautions décrites dans ce manuel.

• Fuji Photo Film Co., Ltd. ne peut être tenu pour responsable des défaillances ou des dommages de l’appareil en cas où il est utilisé dans un environnement qui ne répond pas aux conditions de fonctionnement (alimentation, environnement de l’installation etc.) décrites dans ce manuel.

• Fuji Photo Film Co., Ltd. ne peut être tenu responsable des défaillances ou des dommages de l'appareil dus à des catastrophes naturelles, telles qu'un incendie, un tremblement de terre, des inondations ou des éclairs.

• Aucune partie de ce manuel ne peut être reproduite sans l'autorisation de Fuji Photo Film Co., Ltd.

■ Marques

Les noms de compagnies et de produits sont des marques ou des marques déposées de leurs propriétaires respectifs.

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Conventions

Ce manuel contient des informations importantes concernant l’utilisation en toute sécurité du QuickGene610L. Lisez attentivement ces informations avant d’utiliser et de faire fonctionner le QuickGene-610L. Les significations des symboles de sécurité utilisés sont les suivantes :

WARNING:

BIOHAZARD

CAUTION:

Important : "Important" : signale des remarques importantes concernant l’utilisation et les actions interdites.

Note: "Note" : les remarques concernent les procédures à respecter, elles fournissent aussi des informations complémentaires.

"WARNING" signale des conditions de dangerosité qui, si elles n’étaient pas évitées, pourraient entraîner la mort ou des blessures graves.

"BIOHAZARD" indique l’exposition à un agent biologique ou à une situation qui peut être infectieuse ou nocive.

"CAUTION" signale un danger possible qui, s’il n’était pas évité, pourrait entraîner des blessures corporelles mineures ou légères. "CAUTION" pourrait aussi indiquer un risque de dommage matériel.

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Einführung

Wir danken Ihnen für den Kauf des Nukleinsäureisolierungssystems QuickGene-610L (im Folgenden als „QuickGene-610L“ bezeichnet). Mit diesem Gerät kann durch einfache Bedienung eine DNA- und RNAExtraktion durchgeführt werden. Diese Anleitung enthält wichtige Informationen zur sicheren Verwendung von QuickGene-610L. Lesen Sie die Anleitung vor der Verwendung sorgfältig durch. Bewahren Sie diese Anleitung nach dem Lesen zum späteren Nachschlagen auf.

Umgang mit infektiösen Stoffen

Befolgen Sie bei der Handhabung oder Entsorgung von infektiösen Abfällen die Laborrichtlinien sowie die geltenden Gesetze und Vorschriften, und führen Sie die Verbrennung, Einschmelzung, Sterilisation und/oder Desinfektion entsprechend durch. Wenn Sie die Entsorgung durch Dritte ausführen lassen, beauftragen Sie zugelassene Entsorgungsfirmen für industriellen Sondermüll, und stellen Sie ihnen die relevanten Informationen zur Sondermüll-Abfallbehandlung zur Verfügung.

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■ Ausschlussklauseln

• Der Inhalt dieser Anleitung kann jederzeit ohne Vorankündigung geändert werden.

• Fuji Photo Film Co., Ltd. übernimmt keine Haftung für die Verletzung von Copyright- oder anderen Rechten Dritter, die aus der Verwendung jeglicher Informationen dieser Anleitung entstehen können.

• Fuji Photo Film Co., Ltd. übernimmt keine Haftung für Geräte-Ausfälle oder – Beschädigungen, die durch Installation, Standortveränderung, Veränderungen, Wartung oder Instandsetzung entstehen, sofern diese nicht durch Fuji Photo Film Co., Ltd. oder von Fuji Photo Film Co., Ltd. genannte Auftragnehmer durchgeführt wurden.

• Fuji Photo Film Co., Ltd. übernimmt keine Haftung für Ausfälle und Beschädigungen unserer Produkte, die durch Produkte von Drittherstellern verursacht wurden, sofern diese Produkte nicht von Fuji Photo Film Co., Ltd. geliefert wurden.

• Fuji Photo Film Co., Ltd. übernimmt keine Haftung für Geräte-Ausfälle und -Beschädigungen, die durch Veränderungen, Wartung, Instandsetzung usw. unter Verwendung von Ersatzteilen verursacht wurden, die nicht von Fuji Photo Film Co., Ltd. angegebene Originalteile sind.

• Fuji Photo Film Co., Ltd. übernimmt keine Haftung für Geräte-Ausfälle und –Beschädigungen, die auf die Nichtbeachtung der in dieser Anleitung beschriebenen Vorsichtsmaßregeln und Betriebsverfahren zurückzuführen sind.

• Fuji Photo Film Co., Ltd. übernimmt keine Haftung für Geräte-Ausfälle und –Beschädigungen, die auf den Betrieb des Geräts unter Bedingungen zurückzuführen ist, die den in dieser Anleitung beschriebenen Betriebsanforderungen (Stromversorgung, Betriebsumgebung etc.) nicht entsprechen.

• Fuji Photo Film Co., Ltd. übernimmt keine Haftung für Geräte-Ausfälle und –Beschädigungen, die auf Naturkatastrophen wie Feuer, Erdbeben, Überschwemmung oder Blitzschlag zurückzuführen sind.

• Kein Teil dieser Anleitung darf ohne Erlaubnis der Fuji Photo Film Co., Ltd. reproduziert werden.

■ Warenzeichen

Firmen- und Produktnamen sind Warenzeichen oder eingetragene Warenzeichen der jeweiligen Inhaber.

QuickGene-610L User’s Guide Rev. 1.0 vii

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Warnhinweise

Diese Anleitung enthält wichtige Informationen zur sicheren Verwendung des QuickGene-610L. Lesen Sie diese Informationen sorgfältig durch, bevor Sie das QuickGene-610L in Betrieb nehmen. Die Bedeutung der verschiedenen Sicherheitshinweise wird im Folgenden erläutert:

WARNING:

BIOHAZARD

CAUTION:

Important: „Important“ kennzeichnet wichtige Bedienungshinweise sowie zu unterlassende Vorgehensweisen.

Note: „Note“ kennzeichnet Hinweise, Vorgehensweisen, die befolgt werden sollten, und zusätzliche Informationen zur Bedienung.

„WARNING“ weist auf gefährliche Betriebsbedingungen hin, die zu ernsthaften Verletzungen oder Verletzungen mit Todesfolge führen können.

„BIOHAZARD“ kennzeichnet die Gefährdung durch biologische Agenzien oder Verhältnisse, die infektiös oder gesundheitsschädlich sein können.

„CAUTION“ weist auf gefährliche Betriebsbedingungen hin, die zu leichten bis mittleren Verletzungen führen können. „CAUTION“ kann darüber hinaus das Risiko einer Sachbeschädigung kennzeichnen.

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This chapter describes safety precautions. For your safety and that of others, follow the guidelines provided in the following pages concerning the use of the QuickGene-610L.

About Instrument

WARNING:

Safety Information

• Ignoring the following notations may lead to fire or electric shock.

- Use a power cable that meets your country's standard, or contact your local distributor.

- Connect to a grounded outlet to reach the power source.

- Do not touch the power plug with wet hands

- Do not use the QuickGene-610L with voltage other than the voltage specified on the device.Do not overload the power outlet with too many devices.

- Do not use the QuickGene-610L with a damaged power plug or a loose socket.

- When dust is on the prongs of the power plug or the plug socket, clean with a dry cloth.

- When you disconnect the power plug from the outlet, be sure to hold the power plug itself. Do not pull the power cable.

- For maintenance, disconnect the power plug from the outlet.

- Do not touch the power plug when you hear the crash of thunder.

- When the circuit breaker trips, it may be due to a short circuit. Do not dismantle the QuickGene-610L.

• Do not pour any liquid on the QuickGene-610L. Do not place any

objects containing liquid on the QuickGene-610L. Doing so may cause a device failure, fire, or electric shock.

• In the event the device overheats, starts to smoke or smells strange,

immediately disconnect the power cable.

CAUTION:

QuickGene-610L User’s Guide Rev. 1.0 ix

• Never attempt to remodel the QuickGene-610L without the permission

of Fuji Photo Film Co., Ltd. Doing so may cause fire or electric shock.

• Do not place or drop objects on the QuickGene-610L. Also refrain

from bumping or knocking it, as doing so may cause a failure or malfunction of the QuickGene-610L.

• If any liquid materials are left inside the device, wipe with a

Kimwipe® or paper tissue. Otherwise, the QuickGene-610L may be damaged.

• The front cover will not stay on the way to the upper limit. If you

release the front cover while opening it, it may cause injury or device damage.

• When replacing the Pump Tube, be sure to wear gloves.

• In the event of an ethanol spill, immediately disconnect the power cable.

• Repairs to the QuickGene-610L should only be performed by such

agencies as are specifically authorized by Fuji Photo Films Co., Ltd.

• Only original Fuji Photo Films Co., Ltd. replacement parts should be

used.

• If the equipment is used in a manner not specified by the manufacturer,

the protection provided by the equipment may be impaired.

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About Installation Conditions

WARNING:

CAUTION:

For correct and safe use of QuickGene-610L, install it in a location that meets the following conditions:

• A location where power can be provided. Use an AC100±10% to AC240V±10%, 50Hz or 60Hz outlet.

• A location where the temperature is 15 to 30°C, and humidity is 30 to 80%RH (non condensing).

• Do not install the QuickGene-610L to a location where it can be

splashed with water.Doing so may cause a device failure, fire, or electric shock.

• When relocating the QuickGene-610L, be sure to disconnect the

power plug from the outlet first. Watch your step when you relocate the QuickGene-610L. If the power cable is damaged, this may cause a device failure, fire, injury, or electric shock.

• Do not install the QuickGene-610L in an unstable place such as a

slanted surface or a place subject to vibrations. Doing so may cause injury or device failure.

• Do not install the QuickGene-610L in direct sunlight or close to a

heating device. Doing so may shorten the life of QuickGene-610L, or cause a failure.

• Two or more people are necessary to relocate the QuickGene-610L.

Otherwise, you may hurt your back or hands because of the weight of QuickGene-610L (24kg).

• A location that will support the weight of QuickGene-610L (24kg), that is flat and stable, with no vibration.

• A location away from direct sunlight. Block the sunlight by curtains or blinds as necessary.

• A location which is well-ventilated and not dusty.

• A location where the temperature does not go up and down suddenly.Warming a cold room suddenly or moving the QuickGene-610L from a room with low temperature to a warm room may cause condensation inside the device (condensing), and may have a negative influence on the sample result.

• A location far from water taps, water heaters, humidifiers, air-conditioners and heaters. (Avoid areas of high temperature and high humidity, or low temperature and low humidity.)

• A location far from objects which generate strong magnetic fields (motors, transformers, TV, audio speakers, magnets, etc.). Bringing the QuickGene-610L close to any type of magnetic field may cause a malfunction.

Product Use Limitations

• Fuji Photo Film Co., Ltd. assumes no liability for results regardless of the intended use.

• It is the user's responsibility to validate the performance of QuickGene kits and QuickGene-610L for any case of use.

• The QuickGene-610L is a device for research and study only.

When you use the QuickGene-610L, read this manual thoroughly and follow the safety management regulations of your facilities (laboratory).

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Informations concernant la sécurité

Ce chapitre décrit les précautions de sécurité. Pour votre sécurité et celle des autres, veuillez suivre les indications et recommandations fournies dans les pages suivantes et qui concernent l’utilisation du QuickGene-610L.

A propos des instruments

WARNING:

CAUTION:

• Si les recommandations suivantes ne sont pas prises en compte, un

incendie ou une décharge électrique peuvent se produire.

- Utilisez un câble d'alimentation conforme aux réglementations de votre pays ou contactez votre revendeur local.

- Connectez à une prise électrique mise à la terre

- Ne touchez pas la fiche si vous avez les mains mouillées

- N’utilisez pas le QuickGene-610L avec un voltage différent de celui qui est spécifié sur l’appareil. Ne branchez pas trop appareils sur la prise électrique.

- N’utilisez pas QuickGene-610L si la fiche est endommagée ou si la prise n’est pas solidement fixée.

- Si les broches de la prise ou de la fiche sont poussiéreuses, nettoyez-les avec un chiffon sec.

- Lorsque vous retirez la fiche de la prise, faites-le en tenant fermement la prise. Ne tirez pas le câble.

- Pour tout entretien, débranchez la fiche de la prise murale.

- Ne touchez pas la fiche lorsque le tonnerre gronde.

- Quand le disjoncteur se déclenche, un court circuit peut s’être produit. Ne démontez pas le QuickGene-610L.

• Ne versez aucun liquide sur le QuickGene-610L. Ne placez aucun

objet contenant du liquide sur l'appareil. Vous risquez, en le faisant, de provoquer une panne, un incendie ou une décharge électrique.

• En cas de surchauffe de l’appareil, ou s’il dégage de la fumée ou une

odeur bizarre, débranchez immédiatement le câble d’alimentation.

• N’essayez en aucun cas de remodeler ou modifier le QuickGene-610L

sans l'autorisation du fabricant. Cela pourrait entraîner un incendie ou une décharge électrique.

•

Ne placez – ou ne faites tomber – aucun objet sur le QuickGene-610L. De même, évitez de lui faire subir des chocs ou des coups, cela pourrait entraîner des défaillances ou un mauvais fonctionnement de l’appareil.

•

Si un quelconque liquide s’introduit dans l’appareil, épongez-le avec une serviette en papier, sinon le QuickGene-610L pourrait être endommagé.

• Le capot avant ne tient pas tant qu’il n’a pas atteint sa limite

supérieure d’ouverture. Si vous lâchez le capot avant en l’ouvrant, il risque de blesser quelqu’un ou d’endommager l’appareil.

•

Lorsque vous remplacez le tuyau de pompe, veillez à porter des gants.

• Au cas où de l’éthanol se renversait, débranchez immédiatement le

câble d’alimentation.

• Des réparations du QuickGene-610L ne doivent être effectuées que par

des agences spécifiquement autorisées par Fuji Photo Films Co., Ltd.

• Et seules doivent être utilisées, les pièces détachées originales

provenant de Fuji Photo Films Co., Ltd.

• Si ce matériel est utilisé de manière non spécifiée par le fabricant, la

protection fournie par le matériel peut s’en trouver diminuée.

QuickGene-610L User’s Guide Rev. 1.0 xiii

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A propos des conditions d’installation

WARNING:

CAUTION:

Pour une utilisation correcte et sûre du QuickGene-610L, installez-le dans un emplacement qui répond aux conditions suivantes :

• Un emplacement à proximité d’une alimentation électrique. Utilisez une prise C.A.100 ± 10% à C.A.240V ± 10%, 50Hz ou 60Hz.

• Un emplacement où la température est située entre 15 à 30°C, et ou l’humidité relative est comprise entre 30 à 80%RH (sans condensation).

• Un emplacement qui peut soutenir le poids du QuickGene-610L (24 kg), qui est plane, stable et sans vibration.

• Un emplacement éloigné des rayons directs du soleil. Si nécessaire, bloquez le soleil avec des rideaux ou des stores.

• Un emplacement correctement aéré et sans poussière.

• Un emplacement où la température ne varie pas brutalement. Si vous réchauffez brusquement une pièce fraîche ou si vous déplacez le QuickGene-610L d’une pièce où la température est basse à une autre où il fait plus chaud, de la condensation risque de se produire à l’intérieur de l’appareil et peut avoir une influence adverse sur le résultat de l’échantillon.

• Un emplacement éloigné de robinets d’eau, de bouilloires, d’humidificateurs, d’appareils d’air conditionné et de chauffage. (Évitez les emplacements à température élevée et forte humidité ou à basse température et faible humidité).

• Un emplacement éloigné d’objets qui dégagent un champ magnétique fort (moteurs, transformateurs, TV, haut-parleurs, aimants etc.). Si le QuickGene-610L est à proximité d’un type quelconque de champ magnétique, il risque d’y avoir un dysfonctionnement.

• N'installez le QuickGene-610L dans un emplacement où il pourrait

être éclaboussé par de l'eau. Cela pourrait entraîner une défaillance de l’appareil, un incendie ou une décharge électrique.

• Si vous devez déplacer le QuickGene-610L vers un autre

emplacement, veillez à débrancher l’appareil en premier. Faites attention en déplaçant le QuickGene-610L, si vous marchez sur le câble d’alimentation et qu’il est endommagé, cela peut entraîner une défaillance de l’appareil, un incendie ou une décharge électrique.

• N’installez pas le QuickGene-610L sur un emplacement instable,

comme par exemple, une surface inclinée ou sujette à vibrations. Cela pourrait entraîner des blessures ou un dysfonctionnement.

• Ne placez pas le QuickGene-610L sous l’exposition directe du soleil

ou à proximité d'un appareil de chauffage. Cela pourrait raccourcir la durée de vie du QuickGene-610L ou provoquer un dysfonctionnement.

• Pour déplacer le QuickGene-610L, deux personnes au moins sont

nécessaires. Vous risquez, autrement, de vous faire mal au dos ou aux mains du fait du poids de l’appareil (plus de 20 kg).

Limitations d’utilisation du produit

• Fuji Photo Film Co., Ltd. n’assume aucune responsabilité de résultats quel que soit l’usage voulu.

• Il est de la responsabilité de l’utilisateur de valider la performance des kits QuickGene et du QuickGene-610L dans tous les cas d’utilisation.

• Le QuickGene-610L est un appareil uniquement destiné à la recherche et l’étude.

Lors de l’utilisation de QuickGene-610L, lisez d’abord ce manuel entièrement et respectez les règlements de gestion de risque de votre lieu de travail (laboratoire).

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Sicherheitsinformationen

In diesem Kapitel sind die Sicherheitsmaßregeln beschrieben. Um Verletzungen zu vermeiden, befolgen Sie bitte die Bedienungsrichtlinien für QuickGene-610L auf den folgenden Seiten.

Informationen zum Gerät

WARNING:

CAUTION:

• Bei Missachtung der folgenden Warnhinweise besteht Brand- und

Stromschlaggefahr.

- Verwenden Sie ein Netzkabel, das den geltenden Bestimmungen Ihres Landes entspricht, oder wenden Sie sich an Ihren örtlichen Händler.

- Schließen Sie das Netzkabel an eine geerdete Steckdose an.

- Fassen Sie den Netzstecker nicht mit nassen Händen an.

- Betreiben Sie das QuickGene-610L ausschließlich mit der am Gerät angegebenen Netzspannung. Überlasten Sie die Steckdose nicht durch Anschließen von zu vielen Geräten.

- Verwenden Sie das QuickGene-610L nicht mit einem beschädigtem Netzstecker oder einer lockeren Steckdose.

- Wenn die Kontakte des Netzsteckers oder die Steckdose staubig sind, wischen Sie sie mit einem trockenen Tuch ab.

- Wenn Sie den Netzstecker aus der Steckdose ziehen, fassen Sie stets den Netzstecker direkt an. Ziehen Sie nicht am Netzkabel.

- Ziehen Sie vor der Durchführung von Wartungsarbeiten den Netzstecker ab.

- Fassen Sie den Netzstecker bei Gewitter nicht an.

- Wenn der Schutzschalter auslöst, kann dies an einem Kurzschluss liegen. Zerlegen Sie das QuickGene-610L nicht.

•

Verschütten Sie keine Flüssigkeiten über dem QuickGene-610L. Stellen Sie keine Flüssigkeitsbehälter auf dem QuickGene-610L ab. Dadurch könnte es zu einem Ausfall des Gerätes, zu einem Brand oder Stromschlag kommen.

• Wenn sich das Gerät überhitzt oder wenn Rauch oder ungewöhnliche

Gerüche auftreten, ziehen Sie sofort das Netzkabel ab.

• Versuchen Sie nicht, das QuickGene-610L ohne die Erlaubnis des

Herstellers zu verändern. Dadurch könnte es zu einem Brand oder Stromschlag kommen.

•

Legen Sie keine Gegenstände auf dem QuickGene-610L ab, und lassen Sie keine Gegenstände darauf fallen. Setzen Sie es außerdem keinen Stößen oder ähnlichen Erschütterungen aus, da es dadurch zu einem Ausfall oder zu einer Fehlfunktion des QuickGene-610L kommen könnte.

• Wenn im Gerät irgendwelche Flüssigkeiten zurückbleiben, wischen

Sie es mit einem fusselfreien Papiertuch aus. Andernfalls kann es zu einer Beschädigung des QuickGene-610L kommen.

•

Die vordere Abdeckung rastet nicht vor Erreichen des oberen Anschlags ein. Wenn Sie die vordere Abdeckung loslassen, während Sie sie öffnen, kann es zu Verletzungen oder zu einer Beschädigung des Geräts kommen.

• Tragen Sie beim Auswechseln des Pumpenrohrs stets Handschuhe.

•

Falls Ethanol verschüttet werden sollte, ziehen Sie sofort das Netzkabel ab.

•

Reparaturarbeiten am QuickGene-610L sollten nur von Firmen ausgeführt werden, die von Fuji Photo Films Co., Ltd. ausdrücklich dafür autorisiert sind.

• Dabei sollten nur Original-Ersatzteil von Fuji Photo Films Co., Ltd.

verwendet werden.

• Wenn das Gerät anders als auf die vom Hersteller vorgesehene Weise

verwendet wird, können die Sicherheitseigenschaften des Geräts beeinträchtigt werden.

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Informationen zur Installation

WARNING:

CAUTION:

Für eine problemlose und sichere Verwendung stellen Sie das QuickGene-610L an einem Ort auf, der die folgenden Bedingungen erfüllt:

• Stromversorgung: Verwenden Sie eine Steckdose mit einer Netzspannung von 100 ± 10% bis 240 V ± 10% AC, 50 Hz oder 60 Hz.

• Temperatur 15 bis 30 °C und relative Luftfeuchtigkeit zwischen 30 und 80 % (nicht kondensierend).

• Eine ebene, stabile Oberfläche, die das Gewicht des QuickGene-610L (24 kg) trägt und die keinen Vibrationen ausgesetzt ist.

• Kein direktes Sonnenlicht. Falls erforderlich, schützen Sie das Gerät durch Vorhänge oder Jalousien vor direkter Sonneneinstrahlung.

• Gut belüftete und staubfreie Umgebung.

• Keine abrupten Temperaturschwankungen. Wenn ein kalter Raum schnell aufgeheizt wird oder das QuickGene-610L aus einem kalten in einen warmen Raum transportiert wird, kann im Gerät Kondensation auftreten, die das Probenergebnis beeinträchtigen kann.

• Genügend Abstand von Wasserhähnen, Warmwasserbereitern, Luftbefeuchtern, Klimaanlagen und Heizgeräten. (Vermeiden Sie Bereiche mit hoher Temperatur und hoher Luftfeuchtigkeit oder niedriger Temperatur und niedriger Luftfeuchtigkeit.)

• Genügend Abstand von Gegenständen, die starke Magnetfelder erzeugen (Elektromotoren, Transformatoren, Fernsehgeräte, Lautsprecher, Magneten etc.). Wenn das QuickGene-610L in die Nähe irgendwelcher Magnetfelder gebracht wird, führt dies zu einer Fehlfunktion.

• Stellen Sie das QuickGene-610L nicht an einem Ort auf, an dem es

Spritzwasser ausgesetzt ist. Dadurch könnte es zu einem Ausfall des Geräts, zu einem Brand oder Stromschlag kommen.

• Wenn Sie den Aufstellungsort des QuickGene-610L ändern, stellen

Sie sicher, dass vorher der Netzstecker gezogen ist. Achten Sie bei einer Standortveränderung des QuickGene-610L darauf, wohin Sie treten. Wenn das Netzkabel beschädigt wird, kann es zu einem Ausfall des Geräts, zu einem Brand oder Stromschlag kommen.

• Stellen Sie das QuickGene-610L nicht auf einem unsicheren Ort auf, z.

B. auf einer abschüssigen Oberfläche oder an einem Ort, der Vibrationen ausgesetzt ist. Dadurch kann es zu Verletzungen oder einem Ausfall des Geräts kommen.

• Stellen Sie das QuickGene-610L nicht an einem Ort auf, der direktem

Sonnenlicht ausgesetzt ist oder in der Nähe eines Heizgerätes liegt. Dadurch könnte die Lebensdauer des QuickGene-610L verkürzt oder ein Ausfall verursacht werden.

• Eine Veränderung des Aufstellungsorts des QuickGene-610L muss

von mindestens zwei Personen durchgeführt werden. Ansonsten kann es aufgrund des Gerätegewichts (über 20 kg) zu Verletzungen des Rückens oder der Hände kommen.

Einschränkungen der Produktverwendung

• Fuji Photo Film Co., Ltd. übernimmt keine Haftung für die Ergebnisse, unabhängig vom Verwendungszweck.

• Es liegt in der Verantwortung des Anwenders, die Eignung der QuickGene-Kits und des QuickGene-610L für die jeweils vorgesehene Anwendung zu überprüfen.

• Das QuickGene-610L ist nur für den Einsatz in Forschung und Lehre vorgesehen.

Wenn Sie das QuickGene-610L einsetzen, lesen Sie diese Anleitung sorgfältig durch, und befolgen Sie die Sicherheitsbestimmungen Ihrer Einrichtung (Labor).

xvi QuickGene-610L User’s Guide Rev. 1.0

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Contents

1 Installation

1.1 Installation and Use Conditions........................................................ 1

1.2 Checking the QuickGene-610L and Accessories............................. 3

1.3 Remove the Packaging Materials..................................................... 4

1.4 Overview........................................................................................... 5

1.5 Setup Parts....................................................................................... 8

1.6 Checking QuickGene-610L Operation............................................ 18

2 Basic Functions

2.1 Components ................................................................................... 19

2.2 Working Process of QuickGene-610L ............................................ 20

2.3 Safety Mechanism.......................................................................... 20

2.4 LCD Display Information................................................................. 21

2.5 Main Specifications......................................................................... 22

3 Operation

3.1 Quick Guide.................................................................................... 23

3.2 Turn the Power ON and OFF ......................................................... 24

3.3 Preparation..................................................................................... 25

3.4 Preparation of Lysate (Samples).................................................... 26

3.5 Extraction Operations..................................................................... 27

3.6 When the Front Cover is Opened During PROCESSING. ............. 38

3.7 Operations to Stop Extraction Process........................................... 39

4 Maintenance

4.1 Before Using the QuickGene-610L................................................. 41

4.2 When the QuickGene-610L is left for a Week or More................... 41

4.3 Cleaning the QuickGene-610L ....................................................... 42

4.4 Cleaning the Parts.......................................................................... 43

4.5 How to Replace the Pump Tube..................................................... 43

5 How to Set Parameters

5.1 Parameters..................................................................................... 47

5.2 How to Set/Change Parameters..................................................... 49

5.3 Parameters in EXPERT Mode........................................................ 51

5.4 Changing Extraction Mode Names................................................. 53

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6 Troubleshooting

6.1 Extraction Failure............................................................................ 55

6.2 Typical Failure ................................................................................55

6.3 Error Messages .............................................................................. 56

6.4 When the Buffer is Contaminated................................................... 57

Appendix A

A.1 Usable Power Supply Cords........................................................... 59

A.2 Options ........................................................................................... 60

A.3 About Services................................................................................61

A.4 Repair Service Period of Product ...................................................61

A.5 Customer Support........................................................................... 61

A.6 Notes for Transfer QuickGene-610L............................................... 62

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INDEX

1 Installation

This chapter explains how to install the QuickGene-610L.

2 Basic Functions

This chapter explains the basic functions of the QuickGene-610L.

3 Operation

This chapter explains the extraction procedures.

4 Maintenance

This chapter describes how to maintain the QuickGene-610L.

5 How to Set Parameters

This chapter explains how to set parameters.

6 Troubleshooting

This chapter describes the procedures that should be taken when you suspect device failures, and the messages shown on the LCD.

Appendix A

This appendix describes after-sales services and points to note for transfer.

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xx QuickGene-610L User’s Guide Rev. 1.0

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1 Installation

This chapter explains how to install the QuickGene-610L.

1 Installation

1.1 Installation and Use Conditions

This section explains the installation conditions and operating environment of the QuickGene-610L.

■ Installation conditions

WARNING

CAUTION:

For correct and safe use of QuickGene-610L, install it in a location that meets the following conditions:

•Do not install the QuickGene-610L to a location where it can be splashed with water.Doing so may cause a device failure, fire, or electric shock.

•When relocating the QuickGene-610L, be sure to disconnect the power plug from the outlet first. Watch your step when you relocate the QuickGene-610L. If the power cable is damaged, this may cause a device failure, fire, injury, or electric shock.

•Do not install the QuickGene-610L in an unstable place such as a slanted surface or a place subject to vibrations.Doing so may cause injury or device failure.

•Do not install the QuickGene-610L in direct sunlight or close to a heating device.Doing so may shorten the life of QuickGene-610L, or cause a failure.

•Two or more people are necessary to relocate the QuickGene-610L. Otherwise, you may hurt your back or hands because of the weight of QuickGene-610L (24kg).

• The space shown in the following figure must be allocated.

Top: 30cm

Left: 15cm

from the Bottle Holder Cover)

Right and Rear: 15cm

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1 Installation

• A location where power can be provided.Use an AC100±10% to AC240V±10%, 50Hz or 60Hz outlet.

• A location where the temperature is 15 to 30°C, and humidity is 30 to 80%RH (non condensing).

• A location that will support the weight of QuickGene-610L (24kg), that is flat and stable, with no vibration.

• A location away from direct sunlight.Block the sunlight by curtains or blinds as necessary.

• A location which is well-ventilated and not dusty.

• A location far from water taps, water heaters, humidifiers, air-conditioners and heaters. (Avoid areas of high temperature and high humidity, or low temperature and low humidity.)

■ Use conditions

The following shows the operating environment of the QuickGene-610L.

Items Specifications

Temperature (°C)

Humidity (RH)

Max. wet bulb temperature (°C)

Temperature gradient (°C/Hr) 12 or less (non condensing) Humidity gradient (RH/day) 30 or less (non condensing) Altitude, operating (m) 2000 or lower

Installation (Overvoltage) Category Definition

Pollution Degree Definition

Pollution Degree Pollution Degree 2. Note: Rated for indoor use only.

During operation

During downtime

During operation

During downtime During operation 29 (non condensing) During downtime 29 (non condensing)

15 ～ 30

0 ～ 55

30 ～ 80

10 ～ 80

CAT II Local-level mains (wall sockets). Equipment at this

level includes appliances, portable tools, and similar products. Equipment is usually cord-connected.

Pollution Degree2

Normally only dry, nonconductive pollution occurs. Occasionally a temporary conductivity that is caused by condensation must be expected. This location is a typical office/home environment.

Temporary condensation occurs only when the product is out of service.

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1.2 Checking the QuickGene-610L and Accessories

Check that the following are included in the package. Contact your local representative if you notice any missing part(s).

1 Installation

غ QuickGene-610L

غ Bottle Holder غ Collection Tube

غ Bottle Holder Cover

غ QuickGene-610L

User's Guide

غ Holder Carriage

غ Discharge Tray

غ Wash Buffer Pump

Tube (2)

غ Cartridge Holderغ Holder Stand

Holder

غ Elution Buffer Pump Tube

غ Elution Buffer Cap (Blue) غ Wash Buffer Cap (Purple)

غ Packing (Spare)

غ Waste Tube (WTL) غ Cartridge (CAL2)

غ Wash Buffer Bottle

(500ml)

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1 Installation

1.3 Remove the Packaging Materials

After unpacking the QuickGene-610L from the box, do as follows:

(1) Remove the tape that holds the front cover.

Ta pe

(2) Open the front cover, and remove the following items.

• Clamps for fixing the carrier both side of the Carrier

• Remove the tape that holds the tubes.

Important: Keep the shipping box and 2 clamps.They will be necessary for

transporting QuickGene-610L.

CAUTION:

Open the front cover as far as it will go, using both hands. The front cover will not stay open until it is fully opened. If you release the front cover while opening it, it will drop suddenly and may cause injury or damage to the device.

Clamps

Ta pe

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1.4 Overview

This section explains the name and function of each part in the QuickGene-610L.

■ Front and side view

1 Installation

Operation Panel

Bottle Holder Cover

Front Cover

Handle

■ Rear view

Ventilation Holes

Breaker Checkpoint

Inlet

Power Swtich

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1 Installation

■ Internal view

6 Carrier

1 Wash Buffer Pump

3 Elution Buffer

2 Bottle Holder 4 Discharge Tray Table

No. Name Function 1 Wash Buffer Pump Sends the wash buffer in the Wash Buffer Bottle to the Carrier. 2 Bottle Holder Set the Wash Buffer Bottle and Elution Buffer Conical Tube.

3 Elution Buffer Pump

4 Discharge Tray Table Set the Discharge Tray to the Discharge Tray Table.

5 Holder Stand

6 Carrier Moves automatically during extraction operation. 7 Pressure Head Presses out the air and uses the pressure to flush out solutions. 8 Elution Buffer Nozzle Puts elution buffer into each cartridge. 9 Wash Buffer Nozzle Puts wash buffer into each cartridge.

10 Sensor

11 Cartridge Holder Set cartridges in the Cartridge Holder. 12 Holder Carriage Set the Waste Tube in the Holder Cartriage.

Collection Tube

Holder

Pump

5 Holder Stand

13 Collection Tube Holder

Sends the elution buffer in the Elution Buffer Conical Tube to the Carrier.

Set the Holder Carriage with the Collection Tube Holder and Cartridge Holder mounted, to the Holder Stand.

Checks that the carrier is placed in the correct position. Caution: Do not touch the sensors.

Set Collection Tubes in the Collection Tube Holder.

7 Pressure Head

Elution Buffer Nozzle

Wash Buffer Nozzle

10 Sensor

11 Cartridge Holder

12 Holder Carriage

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■ Operation Panel

1 Installation

BINDING

LCD

STOP RESET

No. Name Function

1LCD

Lamp (LED)

2POWER

3 READY

4 BINDING

5 WASHING

6ELUTION

Button.

7START

8STOP

9 RESET

Displays messages. For the displayed messages, refer to "2.4 LCD Display Information"(→ page

21) and "6.3 Error Messages"(→ page 56).

The green light is on when the power is on. The light is off in the following cases.

•When the power is off

•When an abnormality is detected in the power unit

The green light is on when the QuickGene-610L is ready for operation. The light is off during extraction process or setup.

The green light blinks during binding process or when extraction process is completed.

The orange light blinks when an error is detected. The light is off in other cases.

The green light blinks during washing process or when extraction process is completed.

The orange light blinks when an error is detected. The light is off in other cases.

The green light blinks during collection process or when the extraction process is completed.

The orange light blinks when an error is detected. The light is off in other cases.

Starts a program. The operation cannot be started while the front cover is open, or if an error has

occurred. In the setup mode, changed parameters are saved and quit setup mode.

Halts the program of QuickGene-610L. In the setup mode, changed parameters are not saved and quit setup mode.

Can be used in the following cases to abort the program.

• When pressing the [STOP] button during the extraction process

• When the front cover is opened during extraction process and the process is paused, and then the front cover is closed

WASHING ELUTION

CR MOVE

DISCHARGE

MODE

1112

START

POWER

READY

In the setup mode, changed parameters are not saved and quit setup mode.

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1 Installation

No. Name Function

10 DISCHARGE

11 M ODE

12 CR MOVE

Discharges the old wash buffer and elution buffer remaining in the tubes. Discharges the remaining air from the tubes, and fills the tubes with new wash

buffer and elution buffer. Switch the extraction mode.Each time the button is pressed, the mode is

switched as the following order.

DNA WHOLE BLOOD ISOLATE A ISOLATE B ISOLATE C ISOLATE D ISOLATE E ISOLATE F

Extraction mode names can be changed. Moves the carrier from side to side.Each time the switch is pressed, the carrier

moves from side to side. Can be used while the front cover is closed.

Increments the setting value of the item displayed in the LCD at setup mode. Can only be used at setup mode. Pressing and simultaneously starts the setup.

Decrements the setting value of the item displayed in the LCD at setup mode. Can only be used at setup mode.

1.5 Setup Parts

This section explains how to install the parts included in the package. Install the following parts:

• Power Cable → "

• Pump Tube → "

• Tube → "

• Bottle Holder → "

• Wash Buffer Cap, Elution Buffer Cap, Wash Buffer Bottle (500ml)

■Setup the Wash Buffer Cap and Elution Buffer Cap"(→ page 14)

→ "

• Discharge Tray → "

• Holder Carriage, Holder Stand → "

An empty Wash Buffer Bottle and Elution Buffer Conical Tube are needed to set a Wash Buffer Cap and Elution Buffer Cap. Prepare a Wash Buffer Bottle and an Elution Buffer Conical Tube in order to set a Wash Buffer Cap and an Elution Buffer Cap respectively. One Wash Buffer Bottle (500ml) is included in the package. The recommended bottles are as follows:

Wash Buffer Bottle (500ml) Nalgenunc 500ml Narrow-Mouth Bottle Elution Buffer Conical Tube BD Falcon 50ml conical tube

■Setup the power cable"(→ page 9)

■Setup the Pump Tube"(→ page 9)

■Setup the tubes"(→ page 12)

■Setup the Bottle Holder"(→ page 14)

■Setup the Discharge Tray"(→ page 18)

■Setup the Holder Carriage"(→ page 16)

Type Product name

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■ Setup the power cable

The following explains how to install the power cable.

1 Installation

WARNING

Important: Refer to "A.1 Usable Power Supply Cords"(→ page 59)", for details about the

QuickGene-610L (Rear)

Outlet

Power cable

Ignoring the following notations may lead to fire or electric shock.

•Use a power cable that meets your country's standard, or contact your local distributor.

power cables that can be used in each country.

(1) Connect one of the power plugs of the

power cable to the inlet of QuickGene610L.

(2) Connect the other plug of power cable

to the outlet.

Connect to a grounded outlet to reach the power source.

Inlet

WARNING

■ Setup the Pump Tube

The following explains how to setup the Pump Tube.

Note: When setting the Pump Tube, wear gloves.

(1) Turn the power on.

Power switch

(2) Press the [DISCHARGE] button.

(3) Turn off the power.

Press the [I] side of the switch. When the QuickGene-610L has been initialized, the LCD displays "DNA WHOLE BLOOD" (this name can be changed). It takes about a minute.

Press the [DISCHARGE] button. This operation makes the drive sockets (→ page 10) of the Wash Buffer Pump and Elution Buffer Pump rotate to the position for ease to setup Pump Tubes.

Press the [O] side of the switch.

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1 Installation

(4) Open the front cover.

Hold the handle and open the front cover until the upper limit is reached.

Wash Buffer Pump

Elution Buffer Pump

CAUTION:

(5) Attach the Pump Tube to the pump.

Attach the Wash Buffer Pump Tube (purple) and Elution Buffer Pump Tube (transparent) to the Wash Buffer Pump and the Elution Buffer Pump respectively.

Important: When attaching the Pump Tube,

The front cover will not stay on the way to the upper limit. If you release the front cover while opening it, it may cause injury or device damage.

wear gloves. Do not touch the drive socket in the pump without gloves.

After removal of the pump cover

Drive Socket

(a)Detach the pump cover.

Remove the two screws while holding the Pump Cover so that it does not fall.

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Pump Cover: Rear

Rotor

Pump Tube

Pump Tube Connector

Groove

Slide the Pump Tube connector into groove

Pump Cover: Front

Notch

Notches face front

1 Installation

(b)Attach the Pump Tube to the rear of the

Pump Cover.

"WASH" or "ELUTION" is printed on the bag of the Pump Tubes. Set each type of Pump Tube correctly.

WASH: for Wash Buffer Pump

ELUTION: for Elution Buffer Pump

Important: Check that both ends of

the Pump Tube are placed in the grooves.

(c)Attach the Pump Cover.

ࡐࡦࡊࠞࡃ࡯ⵣ஥

Drive Head Drive Socket

Rotor

Fit together to attach

Turn the rotor vertical position, then re-attach the Pump Cover to the retainer, making sure that the drive head and drive socket are correctly mated. Secure the Pump Cover in place with the original 2 screws.

Important: Check the Pump Cover is

fixed well to the Rear. Then tighten the screws until the spring washer is squeezed.

Spring washe

This completes the setup of the Pump Tube. For the next step, setup the tubes.

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1 Installation

■ Setup the tubes

The following explains how to setup the tubes. Before setting up the tubes, setup the Pump Tubes.(→ "

Note: When setting the tubes, wear gloves.

■Setup the Pump Tube"(→ page 9))

(1) Connect the tubes to the Pumps.

Connect the tube from the instrument to the pump

Connect the thick tube (purple) to the pump

Connect the thin tube (blue) to the pump

(a)Connect the upper tube (from the

instrument) to the Wash Buffer Pump, and connect the lower tube (from the instrument) to the Elution Buffer Pump, by turning as shown in the figure.

(b)Connect the thick tube (upper: purple)

that has the Wash Buffer Cap to the Wash Buffer Pump, and connect the thin tube (lower: blue) that has the the Elution Buffer Cap, by turning as shown in the figure.

(2) Take off the Wash Buffer Cap and

Elution Buffer Cap from the tubes connected to the pumps, by turning Tube Connector.

Note: Hold the cap, and turn the Tube

Connector to disconnect the tube.

Tube Connector

Wash Buffer Cap or Elution Buffer Cap

To avoid contamination, place each Buffer Cap on cleaned bottle or tube.

12 QuickGene-610L User’s Guide Rev. 1.0

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thin tube thick tube

Close the holes using the rubber packing

Inside the instrument

Put the tubes through the holes.

1 Installation

(3) After removing the caps, remove the

tubes through the holes at the lower part of the QuickGene-610L.

Remove the thick tube connected to the Wash Buffer Pump through the lower hole, and remove the thin tube connected to the Elution Buffer Pump through the lower hole.

(4) Push in the rubber packing to the holes

from inside the instrument.

Clamp position

Side view

Push in

Tube

Groove for the thin tube

Groove for the thick tube

Clamp

(5) Hook the tubes with clamps (2 positions).

Push the tubes into the clamps attached to the inside of the instrument. Clamps are located 2positions as shown in the figure.

(6) Put the tubes along the grooves on the

left panel. Right groove is for the thin tube, Left groove is for the thick tube.

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1 Installation

■ Setup the Bottle Holder

The following explains how to set the Bottle Holder.

(1) Attach the Bottle Holder to the

QuickGene-610L.

Set the Bottle Holder at the Roller Catch on the left panel of the instrument.

Bottle Holder

Roller Catch

(2) Set an empty Wash Buffer Bottle and

Elution Buffer Conical Tube in the Bottle Holder.

Wash Buffer Bottle

Elution Buffer Conical Tube

Bottle Holder

■ Setup the Wash Buffer Cap and Elution Buffer Cap

The following explains how to install the Wash Buffer Cap and Elution Buffer Cap.

Wash Buffer Cap (Purple)

Elution Buffer Cap (Blue)

(1) Set the Wash Buffer Cap in the Wash

Buffer Bottle, and the Elution Buffer Conical Cap in the Elution Buffer Conical Tube.

• Set the purple Wash Buffer Cap on the Wash Buffer Bottle (Large).

• Set the blue Elution Buffer Cap on the Elution Buffer Conical Tube (Small).

Note: During buffer bottle replacement,

each tubes can be placed on each clamps temporarily

Side view

Tubes can be placed temporarily

To avoid contamination, do not touch the end of the tubes.

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1 Installation

(2) Connect the Wash Buffer Cap to the

Tube Connector ,thick tube and connect the Elution Buffer Cap to the Tube Connect of thin tube, by turning as shown in the figure.

• Connect the thick tube for the Wash Buffer Pump (upper, purple) and the purple Wash Buffer Cap.

• Connect the thin tube for the Elution Buffer Pump (lower, blue) and the blue Elution Buffer Cap.

Note: Hold the cap, and turn the Tube

Connector to disconnect the tube.

Tube Connector

Wash Buffer Cap or Elution Buffer Cap

(3) Set the Bottle Holder Cover.

Bottle Holder Cover

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1 Installation

■ Setup the Holder Carriage

The following explains how to install the Holder Carriage.

(1) Attach the Holder Stand to the

QuickGene-610L.

Cartridge Holder

Holder Stand

Side of the Cartridge Holder

Put the tab of the Holder Carriage into the notch of the Cartridge Holder.

Holder Carriage Collection Tube Holder

Insert the tabs of the Holder Stand in the slits on the QuickGene-610L.

Note: After attaching the Holder Stand,

check that there is no gap between the bottom of Holder Stand and the QuickGene-610L.

(2) Set the Collection Tube Holder and

Cartridge Holder in the Holder Carriage.

• To set the Collection Tube Holder, put its left and right latches into the notches on the Holder Carriage.

• To set the Cartridge Holder, put the left and right tabs of the Holder Carriage into the side notches of the Cartridge Holder.

Latch

Put the left and right latches of the Collection Tube Holder into the Holder Carriage notches.

16 QuickGene-610L User’s Guide Rev. 1.0

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Side of the Cartridge Holder

The Cartridge Holder notch is set to the Holder Carriage tab.

The long holes can be seen

1 Installation

(3) Check that the Collection Tube Holder

and Cartridge Holder are correctly set in the Holder Carriage.

Check the following.

• The left and right long holes at the front of the Holder Carriage can be seen.

• The left and right tabs of Holder Carriage are set in the notches on the left and right sides of the Cartridge Holder.

Holder Carriage

Set in the notch

Hook

Long hole

Table prong

Ta bl e

The bottom front matches the table front

(4) Attach the Holder Carriage to the

QuickGene-610L.

Hold the handles of the Holder Carriage and attach the Holder Carriage onto the Holder Stand of the QuickGene-610L. Check the following in order to secure the correct setting.

• The left and right hooks of the Cartridge Holder are put into the grooves on the shaft of the QuickGene-610L. There is a little gap between the hook and the groove.

Side view of the hook

Gap

Shaft groove

• The bottom front of the Holder Carriage matches the table front.

• The 2 long holes on the left and right at the front of the Holder Carriage match the prongs of the Table.

Note: When the Carrier is not at the left

end and you cannot attach the Holder Carriage, close the front cover and turn on the QuickGene610L. Press the [CR MOVE] button to move the Carrier to the left end, and then set the Holder Carriage.

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1 Installation

■ Setup the Discharge Tray

(1) Set the Discharge Tray to the Discharge

Tray Table.

Note: When the Carrier is at the left end

and you cannot set the Discharge Tray, close the front cover and turn on the QuickGene-610L. Press the [CR MOVE] button to move the Carrier to the right end.

Discharge Tray

(2) Close the front cover.

1.6 Checking QuickGene-610L Operation

This section explains how to check the operation of QuickGene-610L. After all the parts are installed, check the operation of QuickGene-610L. To check the operation, use sterile distilled water instead of buffers and dissolved samples, and perform the same operation as the normal extraction operation. For the extraction operation, refer to "3 Operation"(→ page 23).

18 QuickGene-610L User’s Guide Rev. 1.0

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2 Basic Functions

This chapter explains the basic functions of the QuickGene-610L.

2 Basic Functions

2.1 Components

The following describes the main components of the QuickGene-610L:

1 Operation unit

2 Pump unit

3 Carrier unit

4 Sample unit

5 Buffer setting unit

No. Component Function

Operate the buttons to select an extraction mode and start/stop an

1 Operation unit

2 Pump unit

3 Carrier unit

4 Sample unit

5 Buffer setting unit

extraction process. The LCD and LEDs display the device status.

Sends the buffers (wash buffer, elution buffer) to the Carrier unit according to the extraction program.

Puts pressurized air and buffers (wash buffer, elution buffer) into the cartridge and performs the extraction process (binding - washing - elution).

By moving the Holder Carriage, the waste liquid and extracted liquid are discharged into the Waste Tube and Collection Tube, respectively.

The buffers (wash buffer, elution buffer) are set here.The buffers set are sent to the discharge unit through the pump unit.

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2 Basic Functions

2.2 Working Process of QuickGene-610L

The following describes the working flow of the QuickGene-610L:

(1) The extraction program is started by using the operation unit.

(2) The wash buffer and elution buffer are sent from the buffer setting unit to the

pump unit.

(3) The wash buffer and elution buffer are sent from the pump unit to the carrier

unit.

(4) The pressurized air, wash buffer, and elution buffer are sent from the carrier

unit into the cartridge (with lysate), and the extraction process is performed.

(5) The extracted sample is eluted to the Collection Tube set in the sample unit.

The waste is discharged to the Waste Tube.

2.3 Safety Mechanism

This section explains the safety mechanisms of the QuickGene-610L. The QuickGene-610L provides the following safety mechanisms:

• Front cover

• Earth leakage breaker

• LCD, Lamp (LED), and buzzer

■ Front cover

When the front cover is open, operations such as extraction processes cannot be performed. Also, if the front cover is opened during the extraction process, it will be temporarily interrupted. However, when the buffer pump is working, the pump will continue to work until the step is completed. Operation can be restarted from this point in the process by closing the front cover and pressing the start button.

Note: During the extraction process, do not open the front cover unless it is necessary.

■ Earth leakage breaker

When there is an electric leak or current surge caused by failures of the QuickGene-610L, the earth leakage breaker will cut off the power supply. Check the breaker condition through the Breaker Checkpoint. When the breaker is OK you can see "I". If the breaker has tripped you will see "O". If the earth leakage breaker trips, contact your local representative.

■ LCD, Lamp (LED), and buzzer

The LCD, Lamp (LED), and buzzer display the operating status of the QuickGene-610L.

20 QuickGene-610L User’s Guide Rev. 1.0

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2.4 LCD Display Information

The following describes the messages displayed in the LCD during normal operations. For the messages displayed in case of errors, refer to "6.3 Error Messages"(→ page 56).

Message Contents FIRMWARE: VxxLxx Shows the Software version.This appears when you turn on the power. WAIT A MOMENT Initializing the QuickGene-610L or disposing of the waste.

DNA WHOLE BLOOD

ISOLATE A

ISOLATE B

ISOLATE C

ISOLATE D

ISOLATE E

ISOLATE F

PROCESSING

COMPLETED

START SW → RESTART

1?2?3?4?5?6?

2 Basic Functions

"DNA WHOLE BLOOD" mode is set to operate on the QuickGene-610L. The operation has not started yet.

"ISOLATE A" mode is set to operate on the QuickGene-610L. The operation has not started yet.

"ISOLATE B" mode is set to operate on the QuickGene-610L. The operation has not started yet.

"ISOLATE C" mode is set to operate on the QuickGene-610L. The operation has not started yet.

"ISOLATE D" mode is set to operate on the QuickGene-610L. The operation has not started yet.

"ISOLATE E" mode is set to operate on the QuickGene-610L. The operation has not started yet.

"ISOLATE F" mode is set to operate on the QuickGene-610L. The operation has not started yet.

Working the extraction program. Or waiting to shift to wash buffer discharges in the second round

(the waiting time can be set). The extraction operation is completed.The extraction result will be

displayed after showing this message for one second. Temporarily stops.This message is displayed in the following cases:

•Temporarily suspended state for the second round wash buffer discharge

•Temporarily suspended state during the multiple times sample processing

•When opening the front cover during the extraction process to interrupt it, and then closing the cover

•When pressing the [STOP] button during the extraction process

This shows the extraction result. "?" indicates one of the following marks and meanings:

(check): Successfully extracted

- (hyphen): Extraction failure (Cartridge is clogged) _ (underscore): No cartridge, or No sample

QuickGene-610L User’s Guide Rev. 1.0 21

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2 Basic Functions

2.5 Main Specifications

The following describes the main specifications of the QuickGene-610L.

■ Main specifications

Items Specifications Model name QuickGene-610L Dimensions 448mm (W) x 332mm (D) x 398mm (H) Weight 24kg Rated input voltage AC100±10% to AC240V±10% Power frequency 50/60 Hz Current consumption 1.0A to 0.5A Phase Single(with 3P grounding pole)

Range of temperature and relative humidity

Samples Max. 6 samples can be processed simultaneously Altitude 2000m or lower (for operation) Noise Sound pressure: 65db (A-weighted sound pressure level) Operation panel display LCD 16 characters x 1 line Sit Indoor use only

Humidity: 30 - 80%RH (non condensing)

Temperature: 15 - 30°C

■ Environmental conditions

EMC FCC Rule Part15b ClassA

EN61326 ClassA EN61000-3-2 EN61000-3-3

Safety UL61010A-1 CSA C22.2 No.1010

EN61010-1 IEC61010-1

CE LV directive (73/23/EEC)

EMC directive (89/336/EEC)

FCC WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference, in which case, the users will be required to correct the interference at their own expense.

Warning This is a Class A product of Electromagnetic Interference (EMI) standard. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures.

This Class A digital apparatus complies whith Canadian ICES-003. Cet appareil mumérique de la class A est comforme à la nomal NMB-003 du Canada.

22 QuickGene-610L User’s Guide Rev. 1.0

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3 Operation

This chapter explains how to turn the power on and off, and explains the extraction process.

BIOHAZARD

CAUTION:

Important: During the operation, put on appropriate gloves and a mask depending on

the operation. In the operation from preparing samples to extraction completion, be careful not to contaminate the samples with sweat, saliva, etc.

3.1 Quick Guide

When performing an extraction operation, check and follow the following:

3 Operation

Wear gloves and a mask during any operation with a Biohazard risk.

Do not place any objects containing liquid such as a tray on the QuickGene-610L. If the liquid spills, it may damage the operation panel.

• Execute discharge first Please execute discharge first.

This operation discharges the old wash buffer, elution buffer, and air remaining in the tubes, and refills new wash buffer and elution buffer. After the discharge operation, dispose of the liquid collected in the Discharge Tray.

→ "

■Discharge"(→ page 25)

• Follow the setting order for parts, and set them correctly. When setting the Collection Tube Holder and Cartridge Holder into the Holder Carriage, follow

the steps below to set them correctly. Failure to do so may cause the sample to spill. For details, refer to "

■Operations before the start"(→ page 27).

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3 Operation

• Set cartridges, Collection Tubes, and Waste Tubes in the correct positions.

Cartridge

123456

Holder

Holder Carriage

Collection Tube Holder

3.2 Turn the Power ON and OFF

This section explains how to turn the power of QuickGene-610L on and off.

■ Turning on the power

QuickGene-610L (Rear)

Breaker checkpoint

Outlet

Inlet

Power cable

Insert the cartridges and tubes starting from the left, in order. If they are not in order, a spill of buffer and/or dissolved sample may occur. This results in an extraction failure, and buffer and/or dissolved sample waste.

(1) Check the power cable connection and

the earth leakage breaker.

• Check whether the power cable is connected to the QuickGene-610L and the outlet.If not, connect it.

• Check that the earth leakage breaker has not tripped. When the breaker is OK you can see "I". If the breaker has tripped you will see "O". If you see "O", disconnect the power cable and contact your local representative.

WARNING

•Connect to a grounded outlet to reach the power source.

•Do not dismantle the QuickGene-610L.Doing so may cause an electric shock.

(2) Press the [I] side of the switch.

Just after the power is turned on, the LCD displays the software version for a few seconds, then

Power switch

changes the display to "WAIT A MOMENT". LCD displays extraction mode name, when the initialization has been completed.

■ Turning off the power

(1) Press the [O] side of the switch.

24 QuickGene-610L User’s Guide Rev. 1.0

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3.3 Preparation

This section explains the preparation of the QuickGene-610L for an extraction process The following procedures must be performed as preparation of the QuickGene-610L:

• Preparation items

• Discharge

■ Preparation items

Before a test run or extraction operation, perform the following:

• Check all the parts are installed ("1.5 Setup Parts"(→ page 8)).

• Prepare the following:

- Gloves

-Mask

- Wash Buffer Bottle (500ml) *The bottle provided with the kit can also be used.

- Elution Buffer Conical Tube

- 1.5ml micro tube

- Waste Tube (WTL) *provided with the kit

- Cartridge (CAL2) *provided with the kit

- Buffer (wash buffer, elution buffer) *provided with the kit

- Lysate (Samples) (For a test run, use sterile distilled water)

3 Operation

(For a test run, use sterile distilled water)

(For the preparation of samples, refer to each kit's handbook.)

Refer to "1.5 Setup Parts"(→ page 8).

■ Discharge

(1) Check the QuickGene-610L has been

cleaned.

For clean up procedure, refer to "■Tasks required when the device is not used for a week or more"(→ page 41) and "4.4 Cleaning the Parts"(→ page 43).

(2) Turn on the power of QuickGene-610L.

"3.2 Turn the Power ON and OFF"(→ page 24)

(3) Open the front cover.

Take the handle and open the front cover as shown in the figure.

CAUTION:

The front cover will not stay on the way to the upper limit.If you release the front cover while opening it, it may cause injury or device damage.

QuickGene-610L User’s Guide Rev. 1.0 25

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3 Operation

Elution buffer

(4) Check that the Discharge Tray is set on

the Discharge Tray Table.

If it is not set, set it.

■Setup the Discharge Tray"(→ page 18)

(5) Put buffer (elution buffer) into a new

Elution Buffer Conical Tube.

The Wash Buffer Bottle provided with the kit can be used as it is.

Important: Buffer (elution buffer) can be

used for a day in the tube inside the QuickGene-610L.

Elution Buffer Concial Tube

Wash Buffer Cap (Purple)

Elution Buffer Cap (Blue)

(6) Remove the Bottle Holder Cover, set the

Wash Buffer Bottle and Elution Buffer Conical Tube (prepared in Step (5)) in the Bottle Holder, and then put caps on both.

Note: Do not touch the inner tubes of the

Wash Buffer Cap and Elution Buffer Cap. In the case of contamination, please refer to "6.4 When the Buffer is Contaminated"(→ page

57).

(7) Set the Bottle Holder Cover.

(8) Close the front cover.

(9) Press the [DISCHARGE] button on the

operation panel.

3.4 Preparation of Lysate (Samples)

Prepare lysate (samples). Make lysate (samples) using the specified kit for each sample. For details, refer to the kit's handbook.

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3.5 Extraction Operations

This section explains each operation before the start of a program and after the completion of a program.

■ Operations before the start

3 Operation

Important: Perform the extraction operation according to the following procedures.

(1) Open the front cover.

Take the handle and open the front cover as shown in the figure.

CAUTION:

(2) Take the Holder Carriage out of the

QuickGene-610L.

Grip the handles, lift the Holder Carriage up, and then take it out.

Note:

(3) Take the Collection Tube Holder and

Cartridge Holder out of the Holder Carriage.

•When the Carrier is not at the left end and you cannot remove the Holder Carriage, close the front cover and press the [CR MOVE] button to move the Carrier to the left end.

•Hold the handles upright and lift them straight up. Failure to do so may make work difficult. Lift the Holder Carriage straight up and move it forward to remove.

The front cover will not stay on the way to the upper limit.If you release the front cover while opening it, it may cause injury or device damage.

Cartridge Holder

Holder Carriage

Collection Tube Holder

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3 Operation

(4) Set the Waste Tubes in the Holder

Carriage.

Put the Waste Tubes into the bottom of the Holder Carriage.

Waste Tube

(5) Open the cover of the Collection Tube

Holder and set the Collection Tubes.

Collection Tube

Put the cap into the groove

Cap

Hold the cover on both sides with your fingers, and rotate the cover

Close the cover

Put the Collection Tube Cap into the groove of the Collection Tube Holder to set it.

Cove

Important: Put the Collection Tubes into

the Collection Tube Holder in the ascending order of the number printed on the Collection Tube Holder from "1".

(6) Close the cover of the Collection Tube

Holder.

Collection Tubes are set in the Collection Tube Holder.

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3 Operation

(7) Set the Collection Tube Holder and

Cartridge Holder in the Holder Carriage.

Cartridge Holder

Side of the Cartridge Holder

Put the tab of the Holder Carriage into the notch of the Cartridge Holder.

Holder Carriage Collection Tube Holder

Latch

• To set the Collection Tube Holder, put its left and right latches into the notches on the Holder Carriage.

• To set the Cartridge Holder, put the left and right tabs of the Holder Carriage into the side notches of the Cartridge Holder.

Put the left and right latches of the Collection Tube Holder into the Holder Carriage notches.

Side of the Cartridge Holder

The Cartridge Holder notch is set to the Holder Carriage tab.

(8) Check that the Collection Tube Holder

and Cartridge Holder are correctly set in the Holder Carriage.

Check the following.

• The left and right long holes at the front of the Holder Carriage can be seen.

• The left and right tabs of Holder Carriage are set in the notches on the left and right sides of the Cartridge Holder.

The long holes can be seen

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3 Operation

Fastener Latch

Pull up

Cartridge Holde

(9) Release the lock of the Cartridge Holder

to open the cover.

Release the left and right fasteners on both sides of the Cartridge Holder and its cover.

(10) Set cartridges in the Cartridge Holder.

Set the cartridges firmly so that they are stable in the Cartridge Holder.

Important:

•Set cartridges in the ascending order of the number printed on the Cartridge Holder from "1".

•When setting cartridges, be careful that the edge of the cartridges does not touch the Cartridge Holder.

123456

Put lysate (samples) into each cartridge.

Cartridge Holder

Holder Carriage

Collection Tube Holder

(11) Check the location of the tubes and

cartridges.

Check that the cartridges, Collection Tubes, and Waste Tubes are not incorrectly inserted as shown in the figure.

If they are not in order, the buffer and/or lysate may

BIOHAZARD

spill, causing a biohazard risk.

(12) Put lysate (samples) into each

cartridge.

Be careful not to touch the cartridges and the Cartridge Holder when transferring the lysate (samples) into the cartridges.

Note: If any lysate is left on the edge of

the cartridge, wipe it with a Kimwipe® or paper tissue.

30 QuickGene-610L User’s Guide Rev. 1.0

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Latch the fastene

Cartridge Holder

3 Operation

(13) Close the Cartridge Holder Cover and

lock it.

Lock the left and right fasteners on both sides.

(14) Attach the Holder Carriage to the

QuickGene-610L.

Holder Carriage

Set in the notch

Hook

Long hole

Table prong

Ta bl e

The bottom front matches the table front

Hold the handles of the Holder Carriage and attach the Holder Carriage onto the Holder Stand of the QuickGene-610L. Check the following in order to secure the correct setting.

• The left and right hooks of the Cartridge Holder are put into the grooves on the shaft of the QuickGene-610L. (There is a little gap between the hook and the groove.)

Side view of the hook

Gap

Shaft groove

• The bottom front of the Holder Carriage matches the table front.

• The 2 long holes on the left and right at the front of the Holder Carriage match the prongs of the Table.

Note: When the Carrier is not at the left

end and you cannot attach the Holder Carriage, close the front cover and turn on the QuickGene610L. Press the [CR MOVE] button to move the Carrier to the left end, and then set the Holder Carriage.

(15) Close the front cover.

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3 Operation

(16) Select an extraction mode.

Press the [MODE] button several times until the target extraction mode appears.

(17) Press the [START] button.

The QuickGene-610L automatically starts the extraction program. LCD displays "PROCESSING" during extracting. Extraction progress can be checked by each lamp (LED).

[Display of extraction results]

, , and

are extraction results

Note:

• Do not open the front cover

during the extraction process (while PROCESSING is displayed in the LCD). If the front cover is opened, the extraction process will be halted. To continue the process, refer to "3.6 When the Front Cover is Opened During PROCESSING."(→ page 38).

• To quit the process, refer to "3.7

Operations to Stop Extraction Process"(→ page 39).

• In some extraction modes,

operations are required during processing. For details, refer to "

■Optional Operation

Procedure [WASH2 program]"(→ page 33).

(18) Two beeps and all the three lamps

(BINDING, WASHING, ELUTION) blinking indicates process completion.

The LCD displays the extraction results.

Display Meaning

Successfully extracted

Extraction failure (Cartridge is clogged)

No cartridge, or No sample

21 3456

The numerics are cartridge numbers

Number 1 to 3: Extracted successfully. Number 4: Extraction failed. Number 5 to 6: There were no cartridges.

(check)

(hyphen)

(underscore)

32 QuickGene-610L User’s Guide Rev. 1.0

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3 Operation

■ Optional Operation Procedure [WASH2 program]

When you chose optional operation mode such as "xxx PLUS" mode (a program added WASH 2 process; see "5 How to Set Parameters"(→ page 47)), QuickGene-610L pauses after first WASH and LCD displays "START SW → RESTART". Then the following steps are continued the program.

(1) Open the front cover.

Open the front cover after LCD displays "START SW → RESTART" with 8 beeps.

CAUTION:

(2) Take the Holder Carriage out of the

QuickGene-610L.

Grip the handles, lift the Holder Carriage up, and then take it out.

Note: Hold the handles upright and lift

them straight up. Failure to do so may make work difficult. Lift the Holder Carriage straight up and move it forward to remove.

The front cover will not stay on the way to the upper limit. If you release the front cover while opening it, it may cause injury or device damage.

(3) Perform the additional operations.

Dispense a reagent, etc.

QuickGene-610L User’s Guide Rev. 1.0 33

For example, dispense a reagent into the cartridges.

Page 56

3 Operation

Holder Carriage

Set in the notch

Hook

(4) Attach the Holder Carriage to the

QuickGene-610L.

Hold the handles of the Holder Carriage and attach the Holder Carriage onto the Holder Stand of the QuickGene-610L. Check the following in order to secure the correct setting.

• The left and right hooks of the Cartridge Holder are put into the grooves on the shaft of the QuickGene-610L. There is a little gap between the hook and the groove.

Side view of the hook

Ta bl e

Long hole

Table prong

■ Operations after the completion

The bottom front matches the table front

Gap

Shaft groove

• The bottom front of the Holder Carriage matches the table front.

• The 2 long holes on the left and right at the front of the Holder Carriage match the prongs of the Table.

(5) Close the front cover.

(6) Press the [START] button.

There may be no noise for a while, depending on the extraction mode or the settings, but the LCD should show a "PROCESSING".

(1) Open the front cover.

CAUTION:

Open the front cover as far as it will go, using both hands. The front cover will not stay open until it is fully opened. If you release the front cover while opening it, it may cause injury or damage to the device.

34 QuickGene-610L User’s Guide Rev. 1.0

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Press down the tab, and remove the Collection Tube Holder.

3 Operation

(2) Remove the Collection Tube Holder.

Note: Grip the handle of the Collection

Tube Holder while pressing down the tab of the cover, and then remove the Collection Tube Holder. If the Collection Tube is tilted, the extracted liquid in it may spill out.Lift the Collection Tube Holder upward, and be careful not to tilt it to pull it out.

Close the cap

Grip the handles and remove

(3) Open the Collection Tube Holder Cover

and close the Collection Tube Caps. Then, take them out.

Note: Check the caps are put on the

tubes completely.

(4) Take the Holder Carriage out of the

QuickGene-610L.

Grip the handles, lift the Holder Carriage up, and then take it out.

Note:

•When the Carrier is not at the left end and you cannot remove the Holder Carriage, close the front cover and press the [CR MOVE] button to move the Carrier to the left end.

•Hold the handles upright and lift them straight up. Failure to do so may make work difficult. Lift the Holder Carriage straight up and move it forward to remove.

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3 Operation

(5) Remove the Cartridge Holder from the

Holder Carriage, and dispose of the cartridges.

Release the lock of the Cartridge Holder to open the cover. One by one pull out the cartridges and dispose of them into a waste container.

Note: When taking cartridges out, be

careful so that the edge of the cartridges does not touch the Cartridge Holder. If the edge of the cartridge touches the Cartridge Holder, wash the Cartridge Holder.

(6) Take the Waste Tubes out.

Waste Containe

Dispose of the Waste Tubes according to appropriate laws and rules.

Dispose of the infectious wastes according to your

BIOHAZARD

(7) Check whether waste liquid remains in

the Holder Stand and Discharge Tray.

If waste liquid remains in them, dispose of it.

Note: When the Carrier is not at the left

end and you cannot remove the Discharge Tray, close the front cover and press the [CR MOVE] button to move the Carrier to the right end.

biological safety manuals.

Discharge Tray

Holder Stand

(8) Set the Cartridge Holder and Collection

Tube Holder in the Holder Carriage, and then install them to the QuickGene610L.

"■Setup the Holder Carriage"(→ page 16)

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3 Operation

(9) Close the front cover.

The operation has been completed. To continue the process, refer to "3.3 Preparation"(→ page 25). When finishing the operation, perform the following tasks:

- Turn off the power of QuickGene-610L.

- Dispose of the remaining buffer.

- Clean the dirty parts. Refer to "4 Maintenance"(→ page 41) for maintenance details.

Important: After QuickGene-610L operation, if it will not be used again for one week or

more, put 14ml of ethanol in the Wash Buffer Bottle and 7ml of ethanol in the Elution Buffer Conical Tube, then execute the discharge operation 4 times. After the discharge operation, if ethanol remains in the tubes, discharge it. For details, refer to " or more"(→ page 41). When the amount of types of kit increases, the contents of wash buffer and elution buffer will differ from the present kits. For details about the remaining liquid in the tubes, refer to "6.4 When the Buffer is Contaminated"(→ page 57), and discharge it.

■Tasks required when the device is not used for a week

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3 Operation

3.6 When the Front Cover is Opened During PROCESSING.

When the front cover is opened during processing, the program is halted and the LCD displays "COVER OPEN". If this occurs while the Wash Buffer Pump or Elution Buffer Pump is running, operation of the device will not be paused until the process for one cartridge is completed. After that, the extraction process can be resumed from the operation panel. To resume the operation after opening the front cover during extraction operation, perform the following procedures. To quit the program, refer to "3.7 Operations to Stop Extraction Process"(→ page 39).

Note: When the front cover is opened, do not remove the cartridges or tubes in the

QuickGene-610L. Also, do not move the Pressure Head. Extraction process restarts from the position where the process was paused. Operations such as discharge may be performed even without cartridges or tubes, which may result in liquid spill.

(1) Close the front cover.

(2) Press the [START] button when "START SW → RESTART" is displayed on the

LCD.

QuickGene-610L will restart from the position where the program is paused.

Restarting operations vary depending on the timing when the front cover is opened (when the operation is paused). The following shows the timing when the front cover is opened and the restart operations.

Process status

During initialization

During binding All operations stop

During washing

During elution

Operations when opening

the front cover

All operations stop

•All operations stop, except for Wash Buffer Pump and Elution Buffer Pump.

•During the wash buffer or elution buffer discharge process, the operations stop after discharge for one cartridge

Operations at restart

The Pressure Head returns to the original position, then the operations restart from the paused position.

•The Pressure Head returns to the original position, then moves to the paused position. Operations restart beginning with the cartridge that was in operation.

•If the operation is paused while applying pressure, pressure application is performed again starting with the applicable cartridge.

If stopped while applying pressure

•The Pressure Head moves to the paused position, and only pressure application restarts.

•If wash buffer or elution buffer was being discharged, operations restart with the next cartridge.

If stopped when pressure application was completed or with no pressure application

•The Pressure Head moves to the next cartridge of the cartridge being processed, then pressure application and wash buffer discharge processes restart.

38 QuickGene-610L User’s Guide Rev. 1.0

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3.7 Operations to Stop Extraction Process

To stop the extraction operation, perform the following procedures:

(1) Press the [STOP] button, or open the front cover.

The LCD displays a message as follows: If the [STOP] button is pressed: "START SW → RESTART" If the front cover is opened: "COVER OPEN"

(2) In the case when the front cover is opened, close the front cover.

(3) When the LCD displays "START SW → RESTART", press the [RESET] button

(not the [START] button) to stop the process.

The mid-process extraction results will be displayed on the LCD.

(4) Perform the operation at extraction completion.

"■Optional Operation Procedure [WASH2 program]"(→ page 33)

3 Operation

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3 Operation

40 QuickGene-610L User’s Guide Rev. 1.0

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4 Maintenance

This chapter describes how to maintain the QuickGene-610L.

When cleaning the QuickGene-610L, wear gloves.

BIOHAZARD

4.1 Before Using the QuickGene-610L

4 Maintenance

Before using the QuickGene-610L, perform the following tasks:

• Keep clean inside the instrument and parts.

■Tasks required when the device is not used for a week or more"(→ page 41)

• Replace the Wash Buffer Bottle and Elution Buffer Conical Tube.

• Supply/Replace buffers (wash buffer and elution buffer)

The buffers must be used within one day from when they are set in the QuickGene-610L.

• Discharge

Discharge the old wash buffer, elution buffer, and air remaining in the tubes, and refill with the new wash buffer and elution buffer.

4.2 When the QuickGene-610L is left for a Week or More

Even after completing the operation and turning off the power, there are buffers (wash buffer, elution buffer) remaining in the QuickGene-610L. When the QuickGene-610L will not be used for a week or more, the remaining buffers may become crystallized, and cause a clog or damage to the tubes.

Discharge the remaining buffers from the QuickGene-610L and clean the inside with ethanol.

■ Tasks required when the device is not used for a week or more

If the QuickGene-610 will not be used for a week or more after the operation, perform the following procedures:

(1) Put 14ml of ethanol in the Wash Buffer Bottle and 7ml of ethanol in the Elution

Buffer Conical Tube, then execute the discharge operation.

For the discharge operation, refer to "■Discharge"(→ page 25).

(2) Repeat the discharge operation 4 times, check the tube is no ethanol

remaining.

(3) Dispose of the waste in the Discharge Tray.

(4) Turn off the power.

■ Tasks required when the QuickGene-610L is used again (after one week or more)

When using the QuickGene-610L again, perform the following procedures:

(1) Put 14ml of sterile distilled water in the Wash Buffer Bottle and 7ml of sterile

distilled water in the Elution Buffer Conical Tube, then execute the discharge operation.

For the discharge operation, refer to "■Discharge"(→ page 25).

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4 Maintenance

(2) Repeat the discharge operation 4 times, check the tube is no water remaining.

(3) Dispose of the waste in the Discharge Tray.

(4) Perform the extraction operation as usual.

4.3 Cleaning the QuickGene-610L

WARNING

This section explains how to clean the QuickGene-610L.

Note: When cleaning the QuickGene-610L, be sure to wear gloves.

The following describes the parts to clean and cleaning methods:

QuickGene-610L (outside)

Pressure Head

Carrier Rail

Note: Do not clean the carrier rail.

Before starting the maintenance work, turn off the power of QuickGene610L and disconnect the power plug from the outlet. Otherwise, it may cause an electric shock.

QuickGene-610L (inside)

Parts to clean Cleaning methods Pressure Head Wipe with a Kimwipe® or paper tissue soaked with ethanol. QuickGene-610L

(outside)

QuickGene-610L (inside)

42 QuickGene-610L User’s Guide Rev. 1.0

Remove the dust lightly with a dry, soft cloth.

Wipe with a Kimwipe® or paper tissue soaked with ethanol or 0.5% sodium hypochlorite solution.

Remove the dust lightly with a dry, soft cloth.

Page 65

4.4 Cleaning the Parts

The following table describes how to clean the parts.

Parts Cleaning methods Holder Carriage Clean the parts using one of the following methods. Cartridge Holder Collection Tube

Holder Bottle Holder

Holder Stand

Discharge Tray

Wash Buffer Pump Tube Elution Buffer Pump

Tube Wash Buffer Cap Elution Buffer Cap Holder Cover Remove the dust lightly with a dry, soft cloth.

4 Maintenance

- Clean with neutral detergent, and then wash out with distilled water. Next, dip into 3% hydrogen peroxide solution at room temperature for 10 minutes. Lastly, wash the solution out with DEPC water, and dry the parts.

- Soak in a 0.1N sodium hydroxide/0.1% EDTA solution overnight, and then wash the solution out with DEPC water, and dry the parts.

- Clean with neutral detergent, and then wash out with 0.5% sodium hypochlorite solution. Lastly, wash out with distilled water, and dry the parts.

- Perform autoclave sterilization (121°C/20min). Note that if autoclave sterilization (121°C/20min) is performed for parts

other than metal parts and packing, the parts may be deformed.

After using the QuickGene-610L, dispose of the liquid collected in the Discharge Tray, and wash the tray with water.

Wipe with a Kimwipe® or paper tissue soaked with ethanol.

4.5 How to Replace the Pump Tube

This section explains how to replace the Pump Tube. If the Pump Tube is continuously used without this maintenance, the Pump Tube may break and the buffer may leak.It is recommended that the Pump Tube be replaced before the required replacement date.

Note: If the QuickGene-610L will not be used for 1 year or more, the Pump Tubes must

be removed from the device.

• Wash Buffer Pump Tube Replacing the Pump Tube is necessary approximately 3 years after the beginning of

QuickGene-610L use, or before performing 10,000 extraction operations.

• Elution Buffer Pump Tube The Pump Tube has a life of about 20,000 extraction operations, which is equal to the

instrument life.

Replace the Pump Tube with the spare one included in the package. If all the tubes have been used, purchase them separately as needed. Note that 20,000 of extraction operations is the instrument life. When purchasing them, contact the Customer Support.

QuickGene-610L User’s Guide Rev. 1.0 43

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4 Maintenance

■ How to Replace the Pump Tube

Perform the following procedures to replace the Pump Tube.

CAUTION:

When replacing the Pump Tube, wear gloves.

(1) Turn the power on.

Press the [I] side of the switch.

(2) Press the [DISCHARGE] button.

Repeat the discharge operation 4 times, check the tube is no liquid remaining. Press the [DISCHARGE] button. This operation makes the drive sockets of the Wash Buffer Pump and Elution Buffer Pump rotate to the position for ease to setup Pump Tubes.

(3) Turn off the power.

Press the [O] side of the switch.

(4) Open the front cover.

Hold the handle and open the front cover until the upper limit is reached.

CAUTION:

The front cover will not stay on the way to the upper limit. If you release the front cover while opening it, it may cause injury or device damage.

(5) Remove the tube connected to the Wash

Buffer Bottle, the tube connected to the Elution Buffer Conical Tube, and the tubes connected to the instrument, from the pumps.

Tubes to the instrument

Tube from the Wash Buffer Bottle

Tube from the Elution Buffer Conical Tube

(6) Detach the pump cover.

Loosen and remove the screws to detach the pump cover.

44 QuickGene-610L User’s Guide Rev. 1.0

Page 67

4 Maintenance

(7) Take off the Pump Tube from the pump,

and attach a new Pump Tube to the rear of the Pump Cover.

Pump Cover: Rear

Rotor

Pump Tube

Pump Tube Connector

Groove

Slide the Pump Tube connector into groove

Pump Cover: Front

Notch

Notches face front

■ How to replace the packing

Perform the following procedures to replace the packing.

Slide the connectors at the ends of the Pump Tube into the grooves of the Pump Cover. Check that the notches on both connectors are facing the front of the Pump Cover. Slide the Pump Tube into place inside the Pump Cover by rotating the rotor. Adjust the rotor to its original vertical position. "WASH" or "ELUTION" is printed on the bag of the Pump Tube. Set each type of Pump Tube correctly. WASH: for Wash Buffer Pump ELUTION: for Elution Buffer Pump

Note: Use the specified Pump Tube.

After this, the procedure is the same as in "How to install the Pump Tube".Refer to " Tube"(→ page 9).

■Setup the Pump

(1) Open the front cover.

Hold the handle and open the front cover until the upper limit is reached.

CAUTION:

The front cover will not stay on the way to the upper limit. If you release the front cover while opening it, it may cause injury or device damage.

(2) Take the Holder Carriage out of the

QuickGene-610L.

Grip the handles, lift the Holder Carriage up, and then take it out.

Note:

•When the Carrier is not at the left end and you cannot remove the Holder Carriage, close the front cover and press the [CR MOVE] button to move the Carrier to the left end.

•Hold the handles upright and lift them straight up. Failure to do so may make work difficult. Lift the Holder Carriage straight up and move it forward to remove.

QuickGene-610L User’s Guide Rev. 1.0 45

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4 Maintenance

(3) Release the lock of the Cartridge Holder

to open the cover. Then raise the tabs of the packing and lift it up.

Raise the tabs of the packing and lift it up.

Squeeze the latches

Ta bs

Latch the fastene

(4) Gently squeeze the new packing into the

eight latches on the Cartridge Holder Cover to set it.

Make the packing tabs face up (when the cover is open).

(5) Close the Cartridge Holder Cover and

lock it.

Fasten the left and right fasteners on both sides to lock the Cartridge Holder Cover.

Cartridge Holder

46 QuickGene-610L User’s Guide Rev. 1.0

(6) Attach the Holder Carriage to the

QuickGene-610L.

(7) Close the front cover.

Page 69

5 How to Set Parameters

This chapter explains how to set parameters. "Set Parameters" means to change the values (such as buffer volume, number of discharges, etc.) for each extraction mode. The parameters set in the QuickGene-610L are stored in 7 separate memory areas. The name of each extraction mode can be selected from 35 names. Therefore, even if an extraction mode name is changed, the parameter values will not be changed.

• → "5.2 How to Set/Change Parameters"（→ page 49）

• → "5.4 Changing Extraction Mode Names"（→ page 53）

5 How to Set Parameters

Important:

5.1 Parameters

Settings of each parameter can be changed from the setup mode. Some parameters are changed from the normal setup mode (USER mode) and others are changed from the EXPERT mode. The following table shows the display sequence, setting range, default value, etc. for each parameter in USER mode.

Display sequenceLCD message

1 BIND PEAK

2 WASH COUNT

3 WASH PEAK

4 WASH VOL1

5 WASH VOL2

6 WASH VOL3

7 WASH VOL4

8 WASH VOL5

9 WASH DIP TM

10 WAS2 WAIT T

11 WAS2 COUNT

12 WAS2 PEAK

13 WAS2 VOL1

14 WAS2 VOL2

15 WAS2 VOL3

16 WAS2 VOL4

17 WAS2 VOL5

18 ELUT VOL

19 ELUT PEAK

20 ELUT DIP TM

• The setup is used to change the parameters, according to new protocol of the kit, etc.Please check the parameters for each kit's handbook.

• In the case of wrong value for parameters, it will cause buffer spill or sample extraction failure.It is the user's responsibility to change the parameters. In EXPERT mode, there are some crucial parameters. Therefore, you must not change any parameters in EXPERT mode without our guidance.

Mode name (The value displays the default)

Setting

range

30 ～ 160

0 ～ 5

30 ～ 160

0 ～ 7500

0 ～ 510

0 ～ 255

0 ～ 5

30 ～ 160

0 ～ 7500

50 ～ 500

30 ～ 160

0 ～ 510

Unit

kpa 120 120 120 120 120 120 120

time(s)3333333

kpa 90909090909090

µl 7500 7500 7500 7500 7500 7500 7500

µl 6500 6500 6500 6500 6500 6500 6500

µl 5500 5500 5500 5500 5500 5500 5500

µl 0000000

sec 0000000

min 0000000

time(s)0000000

kpa 90909090909090

µl 7500 7500 7500 7500 7500 7500 7500

µl 6500 6500 6500 6500 6500 6500 6500

µl 5500 5500 5500 5500 5500 5500 5500

µl 0000000

µl 500 500 500 500 500 500 500

kpa 100 100 100 100 100 100 100

sec 0000000

DNA WHOLE BLOOD

ISOLATE AISOLATE BISOLATE CISOLATE DISOLATE EISOLATE

QuickGene-610L User’s Guide Rev. 1.0 47

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5 How to Set Parameters

The following table shows the meaning of each parameter.

spl

ay se qu en ce

LCD message Parameter name Meaning

BIND PEAK: 30

- BIND PEAK: 160

WASH COUNT: 0

- WASH COUNT: 5

WASH PEAK: 30

- WASH PEAK: 160

WASH VOL1: 0

- WASH VOL1: 7500

WASH VOL2: 0

- WASH VOL2: 7500

WASH VOL3: 0

- WASH VOL3: 7500

WASH VOL4: 0

- WASH VOL4: 7500

WASH VOL5: 0

- WASH VOL5: 7500

WASH DIP TM: 0

- WASH DIP TM:510

WAS2 WAIT T: 0

- WAS2 WAIT T:255

Peak pressure on the sample

Frequency of wash buffer discharges

Wash buffer peak pressure

1st wash buffer discharge rate

2nd wash buffer discharge rate

3rd wash buffer discharge rate

4th wash buffer discharge rate

5th wash buffer discharge rate

Wash buffer immersion management timer

Waiting time

Sets the peak pressure during pressure application.Judgment criteria is "the pressure before pressure application + the pressure set in this item". Pressure application may be stopped before reaching the peak pressure, if air pressure drops suddenly, indicating that all liquid has been discharged.

Can be specified between 30 - 160 kpa in increments of 1kpa.

Set frequency of wash buffer discharges Can be specified between 0 - 5 times.

Sets the peak pressure during pressure application. Judgment criteria is "the pressure before pressure application + the pressure set in this item". Pressure application may be stopped before reaching the peak pressure, if air pressure drops suddenly, indicating that all liquid has been discharged.

Can be specified between 30 - 160 kpa in increments of 1kpa.

Sets the first wash buffer discharge rate. Can be specified between 0 - 7500µl in increments of 250µl. When 0µl is specified, although the Carrier moves to the