Endress+Hauser Analytik Jena UVP Transilluminator Instruction Manual

UVP Transilluminators

Instruction Manual

Analytik Jena US Ultra-Violet Products Ltd.

2066 W 11th Street, Upland, CA 91786 Unit 1,Trinity Hall Farm Estate,
Tel: [phone] Nuffield Road Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web site: www.us.analytik-jena.com

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UV Blocking Cover may be
included with the
transilluminator. The cover is
hinged and adjustable to
varying heights.

Select models include a
control knob for selection of
wavelengths or intensity

Power switch

AJ’s long life filters are
available in a variety of
filter sizes from 15 x
15cm to 25 x 26cm

Introduction

The UVP Transilluminator offers the researcher uniform and intense sources of ultraviolet light
(radiation) in a compact package. The special design emits high intensity excitation UV wavelength for
back-illumination of transparent fluorescent materials. The 302nm UV back-illumination provides a
highly sensitive method to detect double-stranded nucleic acids that have been labeled with fluorescent
dyes such as ethidium bromide or acridine orange. Single stranded nucleic acids may be detected, but
with a lower excitation wavelength more sensitive for nucleic acid visualization than the 365nm model.

The transilluminator is uniquely designed with increased UV intensity and uniformity, instant on
capabilities, no lamp flicker and reduced electrical consumption.

NOTE: Though the manual refers to the midrange UV waveband as 302nm, others refer to this region
as 300nm or 312nm. The spectral output of all these regions is the same.

A Word of Caution: UV Transilluminators are powerful sources of UV radiation that will cause damage
to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly
protected. If not using the transilluminator with an imaging system darkroom, a UV Blocking Cover
should be attached to the transilluminator. Even though this cover blocks the ultraviolet radiation
emitted by the unit, UV Blocking Eyewear should be worn as well.

UVP Transilluminator features:

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Specifications

UVP Transilluminators

Single UV UVP Transilluminator models are designed with either single intensity or variable intensity.
Units are equipped with an electronic ballast.
Dimension: Width: 14” x Depth: 11” x Height: 4.8” (356 x 279 x 122mm)

No. of Intensity
Model Part Number Volt/Hz Wavelength Filter Size Tubes Style

M-15 95-0455-01 100-115/60 302nm 15 x 15cm 4 x 8W Single
95-0455-02 230/50

M-15V 95-0456-01 100-115/60 302nm 15 x 15cm 4 x 8W Variable
95-0456-02 230/50

M-20 95-0447-01 100-115/60 302nm 20 x 20cm 4 x 8W Single
95-0447-02 230/50

M-20V 95-0452-01 100-115/60 302nm 20 x 20cm 4 x 8W Variable
95-0452-02 230/50

M-26 95-0457-01 100-115/60 302nm 21 x 26cm 4 x 8W Single
95-0457-02 230/50

M-26V 95-0458-01 100-115/60 302nm 21 x 26cm 4 x 8W Variable
95-0458-02 230/50

M-26XV 95-0413-01 100-115/60 302nm 25 x 26cm 4 x 8W Variable
95-0413-02 230/50

The mini single UV UVP Transilluminator uses a magnetic core/coil design ballast. The transilluminator
dimensions are: 7.25”D x 10.25”W x 4.5”H. This model has a single intensity.

Model Part Number Volt/Hz/Amp Wavelength Filter Size No. of Tubes

M-10E 95-0180-01 115/60/0.7 302nm 10 x 10cm 5 x 6W
95-0180-02 230/50/0.6
95-0180-03 100/50-60/0.8

UVP 2UV Transilluminators

Units are equipped with an electronic ballast. This model has a single intensity. The physical dimensions
of all models are:

Width: 14” x Depth: 11” x Height: 4.8” (356 x 279 x 122mm)

Model Part Number Volt/Hz Wavelength Filter Size #of Tubes/UV

LM-20 95-0449-01 100-115/60 302/365nm 20 x 20cm 4 x 8W/302nm
95-0449-02 230/50 4 x 8W/365nm

LM-26 95-0459-01 100-115/60 302/365nm 21 x 26cm 4 x 8W/302nm
95-0459-02 230/50 4 x 8W/365nm

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When the UV Blocking Cover is not being
used, UV light may escape through the
holes dedicated to accepting the bracket
pins of the UV Blocking Cover.

 Remove the black safety plugs from

their package

 Insert the safety plugs through the

holes as shown.

Safety Plug

4 x 8W/302nm
4 x 8W/365nm
4 x 8W/254nm

4 x 8W/302nm
4 x 8W/365nm
4 x 8W/254nm

UVP 3UV Transilluminators

Units are equipped with an electronic ballast. The physical dimensions of all models are:

Width: 14” x Depth: 11” x Height: 5.4” (356 x 279 x 137mm)

Model Part Number Volt/Hz Wavelength Filter Size No. of Tubes/UV

LMS-20 95-0417-01 115/60 302/365/254nm 20 x 20cm
LMS-20 95-0417-02 230/50

LMS-26 95-0414-01 115/60 302/365/254nm 21 x 26cm
LMS-26 95-0414-02 230/50

Transilluminator Operation

Safety Precautions

Set-Up

 Place the transilluminator on a level work surface. Be sure that an air space exists around the

bottom of the work surface. This space allows for the proper air circulation through the unit.

 Plug the female end of the power cord into the transilluminator. For 230 volt models, or those

requiring special power cord connectors, ensure that the proper configuration of male
connector or plug has been properly connected to the power cord.

 Plug the male end of the power cord into a properly grounded electrical outlet. The proper

voltage of the transilluminator is found on the product information label. If using the
transilluminator with an imaging system, a jumper cable is required for connecting to the
darkroom. Refer to the imaging system documentation for additional instructions.

 The transilluminator may be equipped with a UV Blocking Cover. This cover allows the user to

safely view and work with the gel/sample on the glass surface while the transilluminator is on
(be sure to wear protective gloves when working with the gel/sample during this process). To
install, remove the brown protective paper from the cover. Insert the bracket pins on the cover
into the holes on the front of the transilluminator.

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If you are not using the transilluminator with an imaging system darkroom, do not operate the unit
without securing the cover. If the cover is missing, a UV Blocking Faceshield must be worn to avoid
UV exposure to the skin. UV Blocking Eyewear should be worn even with the cover in place to avoid
accidental UV exposure.

Using the Transilluminator

Place gel/sample on the filter area. It is recommended to place the gels on a Gel-Tray to protect the
filter surface from cuts and scratches. It is recommended that gloves be worn to avoid contact with gel
and staining agents.

Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath the filter. If
using the transilluminator with an imaging system, the system’s main power is required to be in the ON
position.

When using a transilluminator with multiple UV wavelengths, dial the knob to the appropriate
wavelength setting.

When using the Variable Intensity models, use the variable intensity settings as follows:

 High: allows for UV excitation of fluorophores on gels for routine photography and for

excitation of gels with low sample concentration

 Medium: Excellent for viewing and quick single-band excision
 Low: Allows for positioning and preparation of gels, excising multiple bands and focusing for

photography

The UV Blocking Cover, optionally installed during setup, is adjustable to varying angles to provide
easier access to the filter surface. To adjust the angle, rotate the Phillips head screws (located on the
left sides of the cover’s hinges) clockwise until enough tension has been placed on the hinge to hold the
cover at the desired angle.

After viewing/photographing the sample, turn the transilluminator off.

Permanently Installing the Safety Cover

(Optional)

Your UVP transilluminator includes a UV-blocking safety shield which can be installed temporarily (as
described elsewhere in this manual) or permanently, if desired. The following instructions will explain
the process of permanently installing the UVP-supplied UV blocking cover onto the UVP
transilluminator.

To complete this procedure, the following tools and parts will be required:

 Phillips-head screwdriver
 5/16” nut driver
 3/8” narrow walled nut driver
 (4) hollow 3/8” nuts (supplied with safety cover)

CAUTION: This procedure requires a moderate level of technical competence. If you are not comfortable
working with electronics, tools and/or related components, contact Analytik Jena for assistance.

Note:

The transilluminator images shown in this procedure may differ in appearance from your

transilluminator. However, the procedure is the same.

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Two of Four

Phillips-Head

Screws and

Washers

Transilluminator Filter

Frame with Cover

Transilluminator

Body

Lock Nuts

Metal Spacers

Removal Procedure

When performing the following procedure, place all components (screws, nuts, etc.) in a secure
location, as some will be reused for installation.

1. Turn off the transilluminator by placing the power switch on the front of the unit in the OFF
position. Then, unplug the unit from the wall power.

2. Remove the four Phillips-head screws and washers securing the transilluminator filter frame to the
body of the transilluminator. Lifting up, remove the transilluminator filter frame from the
transilluminator body and place it upside-down on a flat, smooth surface to avoid scratching the filter
glass.

3. Locate the two sets of lock nuts and three metal spacers on the inside of the transilluminator filter

frame, as shown in the image below. Use the 5/16” nut driver to remove the two sets of lock nuts and

spacers.

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Black Tabs

UV Blocking

Cover Hinges

4. Lift the transilluminator filter frame and place the UV blocking cover below the frame. Insert the
black tabs, located on the UV blocking cover’s hinges, through the filter frame as shown.

5. Use the narrow walled 3/8” nut driver to secure the four supplied 3/8” hollow nuts to the black UV
blocking cover hinge tabs.

Installation Procedure

1. With the UV blocking cover hinges at the front of the unit, replace the transilluminator filter frame
on top of the body of the transilluminator.

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Phillips-Head

Screw and

Washer

UV Blocking

Cover

Transilluminator

Body

2. Reinstall the four Phillips-head screws and washers on the sides of the transilluminator to secure
the transilluminator filter frame to the transilluminator body.

Cleaning and Care of the Transilluminator

Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can damage the UV
filter surface).

To protect the filter glass and minimize moisture and liquids on the glass, it is recommended that you
use a UV transmitting Gel-Tray. Refer to the Replacement Parts for ordering information.

Replacing Tubes in the Transilluminator

Disconnect the transilluminator for the electrical supply.

Remove the filter cover: Use a Phillips head screwdriver to remove the four screws on the sides of the
unit. Lift the filter cover off the unit.

Remove the reflectors on the left and right side of the unit: Slide the reflectors up out of the unit.

Remove the tube: Carefully rotate the tube and slide out of the socket. Replace with a new tube by
sliding the tube into the socket and rotating into place.

Insert the reflectors back into place and reattach the filter cover.

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Maintenance/Repair/Technical Assistance

Replacement Parts/Accessories

For replacement parts or components not shown here, contact Analytik Jena Customer Service or place
of purchase. Please have the transilluminator model number available when you call.

Replacement Part Description Part Number Qty. Required

UVP Filter Assembly (M-10) 38-0150-01 1
Filter Assembly (M-15) 38-0313-03 1
Filter Assembly (M-15V) 38-0313-09 1
UVP Filter Assembly (M-20) 38-0313-01 1
UVP Filter Assembly (M-26) 38-0313-02 1
Filter Assembly (M-20V, LM-20, LMS-20) 38-0313-07 1
Filter Assembly (M-26V, LM-26, LMS-26) 38-0313-08 1
Filter Assembly (M-26XV) 38-0163-04 1
UVP Cover, UV blocking (M-10E) 19-0114-01 1
UVP Cover, UV blocking (all other models) 19-0121-01 1
UV Tube, 6 Watt, 302nm midrange (FL6E) – TM-10E 34-0044-01 5
UV Tube, 8 Watt, 302nm midrange (FL8E) 34-0042-01 4
Tube, 8 Watt, 365nm longwave UV (F8T5/BL) 34-0006-01 4
Tube, 8 Watt, 254nm shortwave UV 34-0007-01 4

Accessories Description Part Number

UVP Gel-Cutter 85-0002-01
UVP Gel-Scooper 85-0006-01
UVP Gel-Tray, UV Transmitting, Sm. 11.5”W x 9”D (29 x 23cm) 85-0007-01
UVP Gel-Tray, UV Transmitting, Lg. 16.5”W x 10.5”D (42 x 27cm) 85-0005-01
UVP Gel-Tray, UV Transmitting (25 x 26cm) 38-0296-03
UVP Gel-Ruler, UV Fluorescing 85-0003-01
UVP Spectacles, UV Blocking 98-0002-01
UVP Goggles, UV Blocking 98-0002-02
UVP Faceshield, UV Blocking 98-0002-04

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If you are in North America, South America, East
Asia or Australia:

If you are in Europe, Africa, the Middle East,
Western Asia:

Call [phone]
Customer Service regular business days, between
7am and 5pm PST

Call [phone] Customer regular
business days, between 9am and 5:30pm

E-Mail: [e-mail]

E-Mail: [e-mail]

Fax: Customer Service: [phone]

Fax: Customer Service: [phone]

Write to: Analytik Jena US

2066 W. 11th Street
Upland, CA 91786 USA

Write to: Ultra-Violet Products Ltd.

Unit 1, Trinity Hall Farm Est, Nuffield Rd
Cambridge CB4 1TG UK

Technical Support

Analytik Jena offers technical support for all of its products. If you have any questions about the
product’s use or, operation, please contact AJ’s offices at the following locations.

Note: A Returned Goods Authorization (RGA) number must be obtained from AJ Customer Service
before returning any product.

Warranty

Analytik Jena warrants its Ultraviolet Transilluminators to be free of detects in materials and workmanship
for a period of two (2) years from date of purchase. The foregoing warranty of AJ shall be of no force and
effect if buyer has modified or damaged the product. Tubes and filters are warranted for 90 days.

All warranties or merchantability and fitness for any purpose and all other warranties, expressed or implied,
except those expressly set forth herein, are deemed waived and excluded.

AJ’s duty under the warranty is limited to replacement and/or repair of the defective part at the option of AJ.
AJ shall not be liable for any expenses or damages incurred by the purchaser except as expressly set forth
herein, and in no event shall AJ be liable for any special, incidental or consequential damages of any kind.
This warranty does not supersede any statutory rights that may be available in certain countries.

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