Du Pont SORVALL RT6000B, SORVALL T6000B Operating Instructions Manual

OPERATING
INSTRUCTIONS



SORVALL RT6000B

& T6000B

TABLETOP
CENTRIFUGES

Du Pont Company
SORVALL® Products
Wilmington, Delaware 19898
U.S.A.



SORVALL Centrifuges

PN 07903-3
Issued July 1993

RT6000B & T6000B

This manual is a guide to the use of the

SORVALL® Centrifuges

SORVALL® RT6000B and T6000B

Tabletop Centrifuges

Data herein has been verified and validated and is believed adequate for the intended use
of the centrifuge. Because failure to follow the recommendations set forth in this manual
could produce personal injury or property damage, always follow the recommendations
set forth herein. Du Pont does not guarantee results and assumes no obligation for the
performance of rotors or other products that are not used in accordance with the
instructions provided. This publication is not a license to operate under , nor a recommen­dation to infringe upon, any process patents.

Publications prior to the Issue Date of this manual may contain data in apparent conflict
with that provided herein. Please consider all data in this manual to be the most current.

NOTES, CAUTIONS, and W ARNINGS within the text of this manual are used to emphasize
important and critical instructions.

WARNING: A W arning informs the operator of a hazard or an unsafe practice that could

result in personal injury, affect the operator’s health, or contaminate the
environment.

CAUTION: A Caution informs the operator of an unsafe practice that could result in

damage of equipment.

NOTE: A Note highlights essential information.

© 1988 by E. I. du Pont de Nemours & Co.

ii

SORVALL® Centrifuges

Important Safety Information

Certain potentially dangerous conditions are inherent to the use of all centrifuges. To
ensure safe operation of this centrifuge, anyone using it should be aware of all safe
practices and take all precautions described below and throughout this manual.

WARNING

When using radioactive, toxic, or pathogenic materials, be aware of all characteristics of the
materials and the hazards associated with them in the event leakage occurs during
centrifugation. If leakage does occur, neither the centrifuge nor the rotor can protect you
from particles dispersed in the air. To protect yourself, we recommend additional precautions
be taken to prevent exposure to these materials, e.g., use of controlled ventilation or isolation
areas.

Always be aware of the possibility of contamination when using radioactive, toxic, or
pathogenic materials. Take all necessary precautions and use appropriate decontamination
procedures if exposure occurs.

The use of sealed rotors, buckets and/or sample containers will provide increased protection
from contamination during routine operation. However, these items will not guarantee
contamination protection from accidents resulting in damage to the rotor or buckets. Do not
run hazardous materials in the centrifuge unless placed in a biohazard enclosure and operated
using all appropriate safety precautions.

Safety

Never use any material capable of producing flammable or explosive vapors.

Never exceed the maximum rated speed of the installed rotor; to do so can cause rotor failure.

Always reduce (derate) rotor speed as instructed in this manual whenever:

• the rotor speed/temperature combination exceeds the solubility of the gradient material
and causes it to precipitate.

• the compartment load exceeds the maximum allowable compartment load specified. See
Chapter 3, Operation, page 3-6.

Failure to reduce rotor speed under these conditions can cause rotor failure.

CAUTION

Do not run or precool a rotor at the critical speed, as this will have a detrimental effect on
centrifuge component life. See Chapter 3, page 3-3.

Do not operate the centrifuge with a rotor out of balance. To do so can cause damage to the
centrifuge drive assembly.

Do not operate centrifuge without a rotor properly installed: rotor cover must be on and
locked in place, and the rotor locked to the centrifuge drive. See rotor instruction manual.

The centrifuge can be damaged if connected to the wrong voltage. Check the voltage before
plugging the centrifuge into a power source. Du Pont is not responsible for incorrect
installation.

Always maintain the centrifuge in the recommended manner. See Chapter 4, Maintenance.

iii

Table of Contents

SORVALL® Centrifuges

Table of Contents

Safety Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii

Chapter 1. DESCRIPTION

Centrifuge Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Centrifuge Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2

Chapter 2. INSTALLATION

Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

Page

Chapter 3. OPERATION

Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

Rotor Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Precooling a Rotor in the RT6000B Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

Running Hazardous Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4

Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4

Emergency Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6

Reducing Speed for Rotor Compartment Loads in Excess

of Design Mass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6

Chapter 4. MAINTENANCE

Inspection and Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

REV/MIN x 1000 Indicator Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Motor Brush Inspection and Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

RT6000B CHAMBER TEMP° C Indicator Adjustment . . . . . . . . . . . . . . . . . . . . . 4-4

Parts Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

Service Decontamination Policy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5

Warranty
Index
Decontamination Certificates

iv

APPENDIX

SORVALL® Centrifuges

Table of Contents

List of Illustrations

Figure Page

3-1. Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3-2. Location of Mechanical Override Loop . . . . . . . . . . . . . . . . . . . . . 3-6

4-1. REV/MIN x 1000 Indicator Adjustment . . . . . . . . . . . . . . . . . . . . . 4-3

4-2. Motor Brush Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

4-3. CHAMBER TEMP° C Indicator Adjustment . . . . . . . . . . . . . . . . . . 4-4

List of Tables

Table Page

3-1. Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

v/vi

SORVALL® Centrifuges

A PHOTOGRAPH OF THIS INSTRUMENT WAS NOT TAKEN AND THEREFORE

WILL NOT BE AVAILABLE FOR FIRST PRINTING OF MANUAL

Figure 1-1. SORVALL® RT6000B Refrigerated

Tabletop Centrifuge (T6000B not shown)

vi

SORVALL® Centrifuges

Description

Chapter 1

DESCRIPTION

This manual provides you with the information you need to install, operate and maintain
your SORVALL® RT6000B and T6000B Tabletop Centrifuges. If you encounter any
problem concerning either operation or maintenance that is not covered in this manual,
please contact our Marketing Technical Group for assistance. In the United States,
telephone toll free [phone]. Outside the United States, contact your local
distributor or agent for SORVALL® Products.

Centrifuge Description

The R T6000B combines the features of the tabletop centrifuge and those of the larger
refrigerated centrifuge in a compact tabletop instrument. It is similar to other SORV ALL
Tabletop Centrifuges, with an added refrigeration system that consists of an evaporator/
rotor chamber and a low-temperature condensing unit. The system is designed to
maintain temperature within 1°C, while in the operating range of –5°C to +25°C. The
T6000B is a non-refrigerated tabletop centrifuge.

Both the RT6000B and T6000B feature a closed-loop speed control and have a see­through chamber door that allows rotor calibration and visual inspection of a run in
progress. The chamber door is counterbalanced for easy opening and safe closing. The
lid latch is locked manually by turning the door release knob counterclockwise to the
LOCKED position, then mechanically an interlock engages when the rotor begins spinning
to prevent the chamber door from being opened during operation. The gyro-action, self­centering drive has a diamond hex-shaped drive spindle which assures proper seating of
rotors. The centrifuges also have a slow-start feature that slowly accelerates the rotor from
0 to 600rpm.*

Centrifuge Specifications

Maximum Speed** . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000 rpm

®

Operating Temperature

Range (RT6000B only) . . . . . . . . . . . . . . . . . . . . . . . –5°C to +25°C

Maximum heat output during

operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000 Btu per hour (RT6000B)

*Speed in revolutions per minute (rpm) is related to angular velocity, ω, according to the following:

ω

= (rpm) = (rpm) (0.10472)

Where ω = rad/s. All further references in this manual to speed will be designated as rpm.
** Maximum speed is dependent on rotor and line voltage.

(

2π
60

3000 Btu per hour (T6000B)

(continued)

)

1-1

Description

SORVALL® Centrifuges

Centrifuge Specifications (continued)

Electrical Requirements:

RT6000B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Vac, 60 Hz, 15A, single phase*

220 Vac, 50 Hz, 8A, single phase*

T6000B. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Vac, 60 Hz, 8A, single phase

220 Vac, 50 Hz, 4A, single phase

Dimensions:

Width (RT6000B) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 cm (32 inches)

(T6000B) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 cm (26 inches)

Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 cm (25 inches)

Height . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 cm (12.5 inches)

Mass (Weight):

RT6000B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 kg (187 lbs)

T6000B. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 kg (160 lbs)

*CSA and UL Certified.

Rotors

The table below lists the rotors available for the RT6000B and T6000B Centrifuges. For
more information about other rotor accessories and tubes refer to the most current
SORVALL® Rotors, Tubes, Bottles, and Adapters Catalog.

Rotor Specifications

Maximum
Maximum Maximum Relative
Operating Compartment Centrifugal

Rotor Speed (rpm) Mass (grams) Force (RCF)

A-384 Fixed-Angle
Rotor 6000 27

A-500 Fixed-Angle 6000 115
Rotor

H-1000B Swinging 3200 820
Bucket Rotor

1

1

2

4890

4960

2190

A/S-400 Fixed-Angle 4380 90
Rotor

1 Includes weight of tube holder, tube or bottle, adapter, and tube contents.
2 Includes weight of adapter, tube or bottle, and tube contents.

1-2

2

3000

SORVALL® Centrifuges

Description

RT-6000B

Centrifuge Specifications

Maximum Speed** . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000 rpm

Operating Temperature

Range (RT6000B only) . . . . . . . . . . . . . . . . . . . . . . . –5°C to +25°C

Maximum heat output during
operation

Electrical Requirements:

RT6000B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Vac, 60 Hz, 15A, single phase*

T6000B. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 Vac, 60 Hz, 8A, single phase

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000 Btu per hour (RT6000B)

3000 Btu per hour (T6000B)

220 Vac, 50 Hz, 8A, single phase*

220 Vac, 50 Hz, 4A, single phase

Dimensions:

Width (RT6000B) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 cm (32 inches)

(T6000B) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 cm (26 inches)

Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 cm (25 inches)

Height . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 cm (12.5 inches)

Mass (Weight):

RT6000B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 kg (187 lbs)

T6000B. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 kg (160 lbs)

Rotor Specifications

Maximum Maximum Relative
Operating Compartment Centrifugal

Rotor Speed (rpm) Mass (grams) Force (RCF)

A-384 Fixed-Angle
Rotor 6000 27

A-500 Fixed-Angle 6000 115
Rotor

1

1

Maximum

4890

4960

H-1000B Swinging 3200 820
Bucket Rotor

A/S-400 Fixed-Angle 4380 90
Rotor

2

2

2190

3000

1-3