AP BLOT
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AP BLOTAP BLOT
AUTOMATED BLOT PROCESSOR
USER MANUAL
USER MANUAL
USER MANUALUSER MANUAL
This manual contains DAS reserved information. All rights reserved. Unauthorized copying of this manual or
parts of it is prohibited.
das
srl
Viale Tivoli Km 18,642 – 00018 Palombara Sabina (Roma) - Italy
Tel. (+39 ) 0774 6 6840 – 07 74 637070 - Fax 0774 634 039
E - m ai l: i n f o @d as it al y .c om - ht tp :/ / w w w . d a si ta l y . c o m
Index
Ed.
Indice
0 Rev. 00 18.03.2008 Issue
Date
Data
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USER MANUAL
Description
Descrizione
Doc. N° MDU-AP-26-00-02
Rev.03
Pag. 3
Date: 16.11.2009
Execution
Elaborazione
De Benedictis
A.
Verification
Verifica
Rossi M. Rossi M.
Approval
Approvazione
0 Rev. 01 04.12.2008 Modify CE conformity
0 Rev. 02 13.07.2009 General revision software version 1.9
0 Rev. 03 16.11.2009 General revision software version 1.12
INDEX
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Doc. N° MDU-AP-26-00-02
Rev.03
Date:16.11.2009
Pag. 5
SECTION 1 INTRODUCTION........................................................................................................................................ 7
1.1.
DESCRIPTION ................................................................................................................................................ 7
1.2.
WARRANTY .................................................................................................................................................... 7
1.2.1. Requesting service...................................................................................................................................................... 7
1.2.2. Ordering spare parts..................................................................................................................................................7
1.3.
CE CONFORMITY CERTIFICATE .................................................................................................................. 8
SECTION 2 - GENERAL SAFETY................................................................................................................................. 9
2.1.
GENERAL SAFETY WARNING SYMBOLS .................................................................................................... 9
2.2.
INSTRUMENT WORKING CONDITIONS........................................................................................................ 9
2.3.
INTENDED USE OF THE INSTRUMENT........................................................................................................ 9
SECTION 3 - GENERAL INFORMATION ................................................................................................................... 11
3.1.
INSTRUMENT DESCRIPTION: PURPOSE AND FEATURES...................................................................... 11
3.2.
MAIN FEATURES.......................................................................................................................................... 11
3.3.
TECHNICAL SPECIFICATIONS.................................................................................................................... 11
SECTION 4 - INSTALLATION AND START-UP ......................................................................................................... 13
4.1.
UNPACKING.................................................................................................................................................. 13
4.2.
POSITIONING THE INSTRUMENT............................................................................................................... 15
4.3.
POWER CONNECTION ................................................................................................................................ 15
4.4.
INSTALLING WASH AND WASTE CONTAINERS........................................................................................ 15
4.5.
WORK AREA ................................................................................................................................................. 16
4.6.
PC CONNECTION......................................................................................................................................... 16
4.7.
INSTALLING APEX SOFTWARE ON PC...................................................................................................... 17
4.7.1. Minimum PC requirements ......................................................................................................................................17
4.7.2. Installing APEX software .........................................................................................................................................17
4.7.3. Installing XCALIB software ..................................................................................................................................... 17
4.7.4. Installing USB driver ...............................................................................................................................................18
4.7.4.1. Windows XP .....................................................................................................................................................18
4.7.4.2. Windows 2000 ..................................................................................................................................................19
4.7.5. Installing camera uEye 3.20 driver .......................................................................................................................... 22
SECTION 5 – INSTRUCTIONS FOR USE................................................................................................................... 25
5.1.
GENERAL INDICATIONS.............................................................................................................................. 25
5.1.1. Introduction.............................................................................................................................................................. 25
5.1.2. Turning instrument on.............................................................................................................................................. 26
5.1.3. Running APEX programme ......................................................................................................................................26
5.1.4. LICENCES ...............................................................................................................................................................26
5.1.4.1. Installing licences.............................................................................................................................................26
5.1.5. Main Menu ...............................................................................................................................................................30
5.2.
SESSION MENU ........................................................................................................................................... 32
5.2.1. NEW SESSION......................................................................................................................................................... 32
5.2.2. Work Area Display...................................................................................................................................................49
5.2.2.1. Running a session............................................................................................................................................. 55
5.2.2.2. Displaying Session Results............................................................................................................................... 57
5.2.3. RUN LAST SESSION................................................................................................................................................59
5.2.4. LOAD FROM HOST ................................................................................................................................................59
5.2.5. EXIT ......................................................................................................................................................................... 59
5.3.
MENU DATABASE ........................................................................................................................................ 60
5.3.1. ASSAY ...................................................................................................................................................................... 60
5.3.2. LAYOUTS.................................................................................................................................................................60
5.3.2.1. New layout/Set-up ............................................................................................................................................61
5.3.2.2. Changing Layouts ............................................................................................................................................62
5.3.2.3. Deleting a set-up/layout ................................................................................................................................... 62
5.3.2.4. Cloning a layout ...............................................................................................................................................63
5.3.2.5. Renaming layouts ............................................................................................................................................. 63
5.3.2.1
Configuration protection...................................................................................................................................... 64
5.3.2.2
Report Print Out................................................................................................................................................... 65
5.3.2.6. Importing layouts .............................................................................................................................................65
5.3.2.7. Exporting layouts .............................................................................................................................................65
5.3.3. RESULT ARCHIVES................................................................................................................................................66
5.4.
MENU SETTINGS ......................................................................................................................................... 68
5.4.1. LANGUAGE.............................................................................................................................................................68
5.4.2. INSTRUMENT..........................................................................................................................................................68
5.4.3. USERS...................................................................................................................................................................... 69
5.4.3.1. NEW USER.......................................................................................................................................................70
5.4.3.2. MODIFY........................................................................................................................................................... 71
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5.4.3.3. DELETE ...........................................................................................................................................................71
5.4.3.4. CHANGE PASSWORD.....................................................................................................................................71
5.4.4. CHANGE USER ....................................................................................................................................................... 72
5.4.5. CHANGE PASSWORD ............................................................................................................................................73
5.5.
LABORATORY DATA.................................................................................................................................... 74
5.5.1. DOCTOR DATA....................................................................................................................................................... 75
5.5.2. IMPORT SESSION DATA........................................................................................................................................76
5.5.3. EXPORT SESSIONS DATA......................................................................................................................................76
5.5.4. SETTING COMMUNICATION PORT ..................................................................................................................... 77
5.5.5. COVER BLOCK ....................................................................................................................................................... 78
5.5.6. OPTIONS .................................................................................................................................................................79
5.6.
TOOLS MENU ............................................................................................................................................... 87
5.6.1. MOVEMENT TESTS ................................................................................................................................................87
5.6.2. PRIME TEST............................................................................................................................................................88
5.6.3. LIGHT ON................................................................................................................................................................ 89
5.7.
MENU ? ......................................................................................................................................................... 90
5.7.1. Guide........................................................................................................................................................................ 90
5.7.2. LICENSES................................................................................................................................................................ 90
5.7.3. INSTRUMENT INFORMATION .............................................................................................................................. 92
5.7.4. SOFTWARE COMPONENTS...................................................................................................................................93
5.7.5. ABOUT.....................................................................................................................................................................94
SECTION 6 – SPECIALIST ACCESS ......................................................................................................................... 95
6.1.
SPECIALIST and ADVANCED SPECIALIST................................................................................................. 95
6.2.
SETTING UP A NEW ASSAY........................................................................................................................ 95
6.2.1. Phases Settings......................................................................................................................................................... 98
6.2.2. Changing an Assay.................................................................................................................................................105
6.3.
Copying an existing assay ........................................................................................................................... 106
6.4.
Printing out an assay ................................................................................................................................... 106
6.5.
Importing a protocol ..................................................................................................................................... 106
6.6.
Exporting an assay ...................................................................................................................................... 108
SECTION 7 – MAINTENANCE.................................................................................................................................. 109
7.1.
MAINTENANCE CHECKS & PRECAUTIONS............................................................................................. 109
7.1.1. Cleaning................................................................................................................................................................. 109
7.1.2. Instrument testing................................................................................................................................................... 109
7.1.3. Inspections and checks ...........................................................................................................................................109
7.2.
REPAIRS ..................................................................................................................................................... 110
SECTION 8 - PACKING, TRANSPORTATION, STORAGE AND PUTTING INSTRUMENT “OUT OF SERVICE” . 111
8.1.
GENERAL INFORMATION.......................................................................................................................... 111
8.2.
PUTTING INSTRUMENT “OUT OF SERVICE”/DECOMMISSIONING ....................................................... 111
8.2.1. Temporary Storage.................................................................................................................................................111
8.3.
PACKING THE INSTRUMENT .................................................................................................................... 111
8.4.
TRANSPORTATION.................................................................................................................................... 112
8.5.
STORING THE INSTRUMENT .................................................................................................................... 112
8.6.
DISPOSAL PROCEDURES......................................................................................................................... 112
SECTION 9 – APPENDIX .......................................................................................................................................... 113
APPENDIX A.............................................................................................................................................................. 113
A.1. INSTRUMENT DECONTAMINATION ......................................................................................................... 113
A.1.1. DECONTAMINATION PROCEDURE................................................................................................................... 113
APPENDIX B.............................................................................................................................................................. 115
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1.1. DESCRIPTION
This manual provides Users with all the necessary instructions and maintenance recommendations for a safe
and suitable use of the instrument.
Manual content:
− Section 1 - warranty information, Technical Service and the CE conformity declaration
− section 2 - general safety-warnings
− section 3 - general information, instrument performance and technical data
− section 4 - installation and start up
− section 5 - operating instructions for IFA process
− section 6 - instructions for IFA specialist
− section 7 – instructions for regular maintenance by User(s)
− section 8 - how to put instrument out of service, packaging, transport, storage and decommissioning
− section 9 - appendices
This manual is considered an integral part of the instrument; it should always be available for
User/Maintenance Technician consultation.
For accurate installation, usage and maintenance please read all the instructions carefully before use.
In particular carefully read Section 1 “GENERAL SAFETY WARNINGS”, describing suitable operating
procedures to avoid risk of harm or damage to instrument and personnel.
In case of problems or instrument breakdown, refer to Authorised Technical Service and Repairs only.
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SECTION 1 INTRODUCTION
Pag. 7
1.2. WARRANTY
Each provided instrument is completely tested, and guaranteed for twelve months from delivery.
The warranty is for all mechanical and electrical parts. It is granted only for a proper installation, use and
maintenance in compliance to the given instructions contained in this manual.
Due to a misuse of the instrument the above mentioned warranty does not include the travel and labour-hour
expenses as well as all the accommodation expenses.
The warranty does not include any responsibility for direct or indirect to people and material damages,
caused by improper use and maintenance of the instrument. All parts that are subject to deterioration,
because of their specific use, are excluded from the warranty.
1.2.1. Requesting service
When REQUESTING TECHNICAL SERVICE please refer to this manual and indicate any data displayed on
instrument identification label (model, registration number and year of manufacture).
Please Note: only qualified Technicians with suitable equipment and original spare parts are entitled to repair
the instrument; ordinary maintenance should be carried out by Users as described in this manual.
1.2.2. Ordering spare parts
Parts that are subject to deterioration or that are defective and need to be replaced have to be requested
with the following information:
− Customer purchase order number
− Name/type and/or version of the instrument
− instrument code number
− part code number
− part description
− requested quantity
− name and company address for delivery of ordered original spare parts
Replacing parts with ORIGINAL SPARE PARTS guarantees effective and longer lasting instrument life.
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1.3. CE CONFORMITY CERTIFICATE
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Declaration of conformity in compliance with 98/79/EC
In-Vitro Diagnostic medical devices Directive (IVD)
DAS srl, manufacturer of the Automated Blot Processor “AP-Blot”, In-Vitro Diagnostic Medical Devices risk class,
declares that this instrument complies with the European Directive 98/79/EC (IVD).
The present conformity declaration, as stated in Annex III of the 98/79/EC Directive, is based upon the following
requirements:
• Quality Management System (ISO 9001:2000): Working Procedures and Instructions for
• Design process control;
• Manufacturing process control;
• Tests, Checks and inspections.
• System documentation:
• Technical documents;
• Risk analysis;
• User manual;
• Service manual.
The instrument fully conforms to the following EU directives, which include latest modifications and comply with
national regulations and legislation.
- Directive 2006/95/EC (ex 73/23/CE) regarding low voltage safety and related standards:
- EN 61010-1:2001 - Safety Precautions for electrical, measuring, control and laboratory equipment
- Directive 2004/108/CE (ex 89/336/EC) - directive regarding electromagnetic compatibility and related
standards:
- CEI EN 61326-1 (for electrical, measuring, control and laboratory equipment)
- EN 55011 (Emission RF)
- EN 61000-3-2 (Harmonics)
- EN 61000-3-3 (Flickers)
Palombara Sabina, October 27th, 2008
Mario Orsini
Managing Director
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SECTION 2 - GENERAL SAFETY
2.1. GENERAL SAFETY WARNING SYMBOLS
In this manual the following symbols stand for danger or warnings:
General DANGER symbol. Indicates that not following relative instructions and warnings can
be a serious safety risk.
Indicates ELECTRICAL VOLTAGE, which could cause death upon contact. Covers with this
symbol can only be removed and replaced by qualified people and only after electrical power
disconnection.
Indicates that instrument is used with reagents and other DANGEROUS CHEMICAL
SUBSTANCES, corrosive, irritating or noxious, which could damage health.
Indicates that the instrument handles potentially infectious samples (e.g. body fluids such as
urine) which could cause INFECTION/CONTAMINATION. Always observe the general safety
standard rules for the presence of these biological substances.
Indicates that not following correct instructions could cause damage to the instrument and/or its
proper functioning.
Indicates important information concerning the instrument or a section of the document that is
important to read with particular attention.
2.2. INSTRUMENT WORKING CONDITIONS
The instrument is intended to be used in the following working conditions:
− as In Vitro Diagnostic (IVD) medical device as specified in the technical data
− with chemical reagents and accessories supplied and/or declared to be compatible with the
instrument
− at a specific temperature and humidity level according to the details given in this manual
− not to be used and powered in a potentially explosive or fire hazardous environment
The instrument should onlybe be used as described in this manual.
Any other use is regarded as improper
2.3. INTENDED USE OF THE INSTRUMENT
The instrument has to be used for the expected purpose and in perfect technical conditions, by qualified
personnel, in working conditions and following maintenance operations described in this manual.
This manual contains instructions for qualified operators.
− Qualified Users have to ensure that environmental conditions are suitable and that installation, use
and maintenance are correct and in accordance with general safety rules, as well as following those
specific precautions described in this manual.
− No User is qualified or entitled to repair the instrument.
− Only Qualified and specifically trained Technicians are entitled to service and repair the
instrument with original spare parts.
Modifications of the instrument are not allowed. The user is liable for any improper
modifications and for the consequences deriving from them.
For extraordinary maintenance, ask for licensed service centers. The maintenance will be carried
out by Specialized Technicians that will be able to repair the instrument using original spare
parts.
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SECTION 3 - GENERAL INFORMATION
3.1. INSTRUMENT DESCRIPTION: PURPOSE AND FEATURES
The AP-Blot instrument dispenses, rinses and reads blots on immuno-enzimatic analysis strips.
It can also dispense, incubate and wash organic samples (pre-diluted when required) and specific reagents.
Up to 48 strips can be processed.
3.2. MAIN FEATURES
Aim
Samples
Reagents
Reaction Areas
Dispensation
Washing
Image acquisition
External liquid bottles
Immuno-enzimatic
management software
Automatic analysis of Dot-Blot and Western Blot tests, capable of processing up to 10 different
protocols simultaneously
Maximum capacity: 48 samples in 13x100mm tubes.
Programmable Automatic Predilution for each sample directly carried out on trays
Modular Tray for controls, conjugates, chromogens, stop solutions, common reagents (e.g.:
sample dilution solutions)
6 racks for 8 different positions: 48 strip total capacity
Three axis arm (X, Y, Z1, Z2) with two independent probes, one with liquid level sensor
Probe washing: external immersion wash in-well; internal wash with Buffer solution.
Strip: shaking plane washing system
High resolution colour camera
A probe washing WASH bottle container, a WASH BUFFER container for blot washing and a
WASTE bottle container.
Each bottle has a level sensor. Each container is inside the instrument
PC software is Windows XP and 2000 compatible capable of:
− Setting up work lists
− Automatically executing all session phases (dispensation, incubation, washing and
readings)
− Creating final reports and result archive
Pag. 11
3.3. TECHNICAL SPECIFICATIONS
Sample rack
Reagent rack
Liquid Containers
Strip Holders
Dispensation Probe Unit
Strip Washing
Image acquisition
Dispensation
Software
Minimum PC
Requirements
Power Requirements
Optional
Measurements (in cms)
Weight
48 sample tubes (Ø 13mm)
6 x 100ml phials and 8 control positions
2 bottles for wash buffer and waste. Both with liquid level sensors
6 slides (8 strips per slide) to process up to 48 strips on shaking plane
1 dispensation probe and 1 aspiration probe
Liquid dispensation and aspiration via two instrument probes
High Resolution Colour Camera (3.3 Megapixels) mounted above Probes
Up to 2500 µl with a 1 µl resolution
Windows 2000 and XP compatible to set up work list, reports and result archive.
Loading work lists and transmit results via Host Computer interface (LIS)
− 200 MB free hard disk space
− CD-Rom reader
− RAM 512 MB
− Pentium 4
− Standard video card:VGA 1024x768 16 million colours
− Free USB 2.0 port
230/115 VAC, 50-60Hz, 150W
Bar Code Reader (BCR) for samples with position ID
79 x 61 x 65 h
78 Kg
SECTION 4 - INSTALLATION AND START-UP
7
2
3
4
5 6
1
When installing and setting up the instrument, carefully follow safety warnings and general rules
described in Section 1.
4.1. UNPACKING
The instrument is packed with a wooden box. To unpack the instrument follow the instructions described below:
a) Remove the 2 plastic covers (1-2) from the instrument right side and the other ones from the left side. Screw in
the 4 handles on both sides (3-4) for manual instrument placement. Once in position remove the handles e put
back in place the plastic covers.
b) Remove the 4 screws that hold the instrument on to the wood base (5-6)
c) Remove screw and bracket (7), screw in the same place using the screw removed before
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9
11
10
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d) Remove screws (8,9) and related brackets
e) Remove the Z block holder (10) as indicated
f) Remove the protection cap from the camera (11)
g) Remove the blocking tools (12) from the oscillating plane
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If the instrument was already in use check for the DECONTAMINATION DECLARATION
(see Appendix A of this manual).
KEEP THE EMPTY BOX! This box is designed and manufactured specifically for this
instrument only and can be used for future transportation.
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4.2. POSITIONING THE INSTRUMENT
The instrument has to be placed on a level bench (table or equivalent work surface) and in an
environment where the temperature ranges between 15
80% and the instrument is protected from direct sun light.
o
C and 35 o C, humidity levels remain under
4.3. POWER CONNECTION
Once the instrument has been placed in position proceed with plugging it into the correct power source 230VAC or
115VAC (see label) using the cables supplied with instrument.
Warning: check that electrical power source corresponds to the source indicated on the label stuck on
the side of the instrument!
Warning: check that fuses correspond to those indicated on the side of the instrument!
230 VAC no 2 x 5A fast fuses
115 VAC no 2 x 10A fast fuses
4.4. INSTALLING WASH AND WASTE CONTAINERS
Figure 1 shows how wash/waste containers are connected up to the instrument.
T1
R
T1 Wash: probe wash solution bottle
T2 Buffer: strip wash solution bottle
T3 Waste: waste liquid bottle
PROCEDURE
Level sensors connector
Before switching on instrument:
− fill T1 and T2 bottles with solution according to method requirements
− connect empty T3 (waste) bottle container
− connect tubing to appropriate containers as indicated in figure 1
− check tubings are correctly connected and in good condition
− plug level sensor lead to instrument socket (connector R)
Note: at the end of a session or before performing maintenance procedures: run wash session for
instrument.
T2
fig. 1 - Hydraulic circuit
T3
port
4.5. WORK AREA
Probe
washing
basin
48 position Sample rack Control - reagent rack
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Bottles
Camera Calibration Area
Strip holder trays
fig. 2- AP-Blot Work Area
4.6. PC CONNECTION
The instrument is equipped with a USB port for PC connection. To install the USB driver please see par. 4.7.4.
Plug, fuses, switch
fig. 3- Electrical socket/ 2.0 USB port
2.0 USB communication
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4.7. INSTALLING APEX SOFTWARE ON PC
4.7.1. Minimum PC requirements
− Microsoft Windows 2000 or XP
− 200 MB free hard disk space
− CD-Rom reader
− 512 MB RAM
− Pentium 4
− Standard video card VGA 1024x768 16 million colours
− 2.0 USB port
4.7.2. Installing APEX software
Insert “APEX software” CD into computer. APEX software is self-installing. If this automatic installation process does
not begin, it can be launched manually by clicking on setup.exe file which appears on the display.
Choose programme display language (English or Italiano). NB: this choice is only valid for the installation.
Click “Next” until installation procedure window appears:
•
Click on “Complete” to choose default file - C:\Programmes\APEXSuite\APEX;
•
Click on “Personalise” to change default file destination path.
After installing APEX software continue to install USB driver as described later (see para 4.7.4).
At the end of the installation launch APEX software by double clicking on APEX icon which appears on the desktop.
NB: User can access all APEX suite programmes from the “APEX” group from main menu (by selecting Start>all
programmes>APEX Suite).
4.7.3. Installing XCALIB software
NB: XCALIB software is the AP-Blot Service software
Insert “XCALIB software” CD into computer. XCALIB software is self-installing.
If this process does not automatically start then click on the setup.exe file from the CD displayed.
Choose language after installation has begun (English or Italiano). NB: this choice is only for the installation
procedure.
Click on “Next” until installation choice window is displayed:
•
Click on “Complete” to install default file C:\Programmes\APEXSuite\XCALIB;
•
Click on “Personalise” to change installation file path destination.
When software is installed continue by installing USB driver as described later (see para 4.7.4).
At the end of installation procedure, launch XCALIB software by clicking on XCALIB icon which appears on the
desktop as part of the “APEX Suite” group. Or From the main menu programme bar (select “start>all
programmes>APEX Suite)
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4.7.4. Installing USB driver
The instrument communicates with PC through a convertor USB port (RS232-USB).
2 driver installation procedures are required: the first to communicate with the electronic part of the instrument and the
second to emulate (=imitate) a standard PC serial port.
When these drivers have been correctly installed a new serial port has been added to the PC (e.g.: COM5).
4.7.4.1. Windows XP
Connect instrument to the PC USB. The “New Hardware Guided Installation” procedure will automatically start up
with the following display:
fig. 4
Select “No, not yet” in answer to question: “Connect to Windows Update to search for new software?” then click on
“Next”
Select “Install from list or specific path” then click on “Next”.
fig. 5
Click “BROWSE” to find file containing USB driver, then click OK and continue.
If Windows XP is configured to warn of the installation of a non-WHQL certified driver a “Warning! Message” will
appear: click on “Continue anyway”.
The Windows programme displays a note at the end of the installation - click on “END” to complete.
The same procedure now automatically begins to install a serial port emulator – follow instructions as above.
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When both drivers have been installed, open Control Panel select System then click on Hard Ware file then Hardware
Resources.
The new USB/Serial port should appear as an additional serial port called “USB Serial Port” (see below for example –
called “COM7” in fig. 6).
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fig. 6
Now APEX or XCALIB software can be launched and the appropriate communication port can be selected.
4.7.4.2. Windows 2000
Connect instrument to PC USB. The “New Hardware Guided Installation” procedure will automatically start up.
Click on “Next” to continue.
Fig. 7
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Select “Search for appropriate Driver”
Continue by clicking on “Next”.
Select only “Specify path” and click on “Next”.
fig. 8
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fig. 9
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Click on “BROWSE” and specify USB driver path file in display which appears below:
When file with USB driver has been opened, select FTDIBUS.INF and then click “Next” to continue installation.
A Windows display announces successful installation and the USB is ready.
A similar process begins for the serial port emulator driver. Follow similar procedure.
When both drivers have been installed open Control Panel, select System and then Hardware file and open the
“Hardware Resources”.
The “new” USB/Serial Port should appear as an additional communication port: “USB Serial Port” (e.g.: COM5 as
shown below Fig.12).
Fig. 10
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Fig. 11
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4.7.5. Installing camera uEye 3.20 driver
4.7.5.1. Windows XP
To install uEye 3.20 camera driver proceed as follows:
1) Disconnect instrument from PC 2.0 USB
2) From driver CD click on display which appears: “CAMERA DRIVER uEye_3.20” then select file
"uEyeBatchInstall_320.exe".
Pag. 22
fig. 12
3) Select “Install the uEye USB driver” option by typing in ‘2’. Press Enter
fig. 13
4) When installation is successfully completed a confirmation message will appear: “uEye driver installed
correctly”. To continue press Enter.
fig. 14
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5) Now connect APBLOT instrument to the PC 2.0 USB port.
6) When new hardware is recognised “Connect to Windows Update to search for new software?” appears and
now select “No, not yet” - then click on “Next” to continue.
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7) Select “Automatic software installation” and click “Next”.
fig. 15
fig. 16
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8) If a display shows incompatibility with Windows XP click on “Continue” and installation continues:
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fig. 17
9) The following display will appear when installation has been successful:
fig. 18
10) Click on End
11) NB: sometimes camera driver installation software requests procedure to be repeated from point (6)
12) Now launch APEX or XCALIB software and choose appropriate communication port.
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SECTION 5 – INSTRUCTIONS FOR USE
5.1. GENERAL INDICATIONS
The instrument is used with chemical reagents and other DANGEROUS CHEMICAL SUBSTANCES
(corrosive, irritant and harmful) which can damage health. When this label is present particular care and
attention must be taken to follow all manufacturer recommendations.
The instrument handles potentially infected samples (urine and human serum etc.) therefore INFECTIONS or
CONTAMINATION might occur.
Take all proper precautions in the presence of such biological substances.
Use protective clothing, gloves and goggles.
Wear protective clothing, goggles and gloves
At the end of a work session, check condition and carefully clean work panel and accessories with a cloth
5.1.1. Introduction
soaked in a 0.5% sodium hypochlorite solution
Any waste material produced during the sessions and cleaning process can be dangerous – with potential
risk of contamination for personnel and other matter.
Dispose of such waste material in accordance with local laws and regulations
The following table shows the main way this instrument functions.
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Run Program
WORK SESSION OTHER FUNCTIONS
New session
Run last session
Load from Host
Run work session or
other function?
Database
Settings
Tools
?
fig. 19– General functions
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5.1.2. Turning instrument on
Press the on/off button on the right hand side of instrument. There are three lights on the front of the machine:
POWER
RUN
ALARM
Please note: The position where the liquid error has been found will be displayed on the PC screen.
Green light Indicates that the instrument is switched ON
Yellow light Indicates a programme is running
This shows that at least one liquid error has been found: the tip has not found any
Red light
liquid in one of the containers (sample tube or reagent container) on the work area
(Note 1)
5.1.3. Running APEX programme
The Apex programme icon appears on the desktop. Double click on it to launch programme.
Apex
Fig. 20 – Programme icon
5.1.4. LICENCES
5.1.4.1. Installing licences
Double click on programme icon to begin English language programme – the following window display appears asking
if any new licenses are to be loaded (Fig. 29 below):
Fig. 21 – License request
Click No to exit this part of the programme
Click Yes to display Licenses window which lists possible licenses to be loaded as shown below Fig.22
fig. 22- Licenses Window
Please note: The license is only valid for one instrument.
Please note: Loading a Demo License for this specific instrument overwrites any other licenses.
Any other licenses present (if required) should be re-loaded.
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Select Load new license to open display and CD installation folder and then click “Licenses”.
These are contained in an *.xml file and can be opened by selecting Apri (Fig. 23) or by clicking Demo mode and
selecting type of instrument to function in Demo Mode – without being connected to a PC.
Choose License for instrument and click “Apri/Open”:
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Fig. 23– Selecting the license
Click Deselect all to de-select license and not load it.
Click OK to load license.
fig. 24
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Select OK to show license in the licenses window as shown below in Fig. 33
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fig. 25
When Licenses window is closed (exit) the following form will be displayed automatically.
Insert both login and password and select Enter.
Fig. 26- Software access form
After inserting login and password the Communication port settings window will open to input communication port
setting from set of pull-down lists Fig. 26
Fig. 27 - Port settings
Select Set to store selected communication port. This selection is confirmed as shown below Fig. 27
Select Cancel to exit this part of programme and leave original setting.
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Click OK. The Main Menu will appear if the selected port is correct and the instrument is on.
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Fig. 28– Confirmation window
A Warning message will appear (Fig. 28) asking if communication port should be changed. If selection is incorrect, or
if Port is correct, but the instrument is not turned on.
Fig. 29– Change selected port request
If the instrument is turned off, turn it on and select No.
The Main Menu will now appear. Click on “light” icon (fig. 30) if the port is correct the light will come on.
fig. 30- Main Menu On/Off light
If instrument is on but the light does not flash ‘on’ this indicates that software is only in DEMO mode.
Change port selection.
The light will turn on as soon as the correct port is selected.
Click on Light Icon to turn light off.
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5.1.5. Main Menu
The Main Menu appears as below in Fig. 39:
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The following diagram (Fig. 32) shows the operating menu and how it works:
Session
New session
Run last session
Load from host
Exit
Database
Result archives
Assays
Layout
Fig. 31 -Main Menu
Settings
Language
Instrument
Work area
Users
Change User
Change password
Laboratory data
Doctor data
Import session data
Tools
Movement tests
Prime Test
Light on
?
Guide
Licenses
Instrument information
Software Component
About
Export session data
Communication port
Cover block
Option
Fig. 32– Functions Menu
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