Das AP-Blot Service manual
AP
AP----BLOT
APAP
BLOT
BLOTBLOT
SERVICE MANUAL
SERVICE MANUAL
SERVICE MANUALSERVICE MANUAL
This manual contains DAS reserved information. All rights reserved. Unauthorized copying of this manual or parts
is prohibited.
das
srl
Viale Tivoli Km 18,642 – 00018 Palombara Sabina (Roma) - Italy
Tel. (+39) 0774 66840 – 0774 637070 - Fax 0774 634039
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Ed.
Index
Indice
AP Blot– SERVICE MANUAL
Date
Data
SECTION 0
INTRODUCTION
Description
Descrizione
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Execution
Elaborazione
Verification
Verifica
Pag. 3
Approval
Approvazione
0 Rev. 00 29.04.08
0 Rev. 01 02.04.09
Important Notes:
To avoid errors during maintenance and repairs, carefully read the entire manual before
attempting any work on the instrument.
Issue
General revision
Pay particular attention to the “GENERAL SAFETY WARNINGS” in Section 1 and follow instructions for specific
operations precisely as indicated in this manual.
CONTENTS
AP Blot– SERVICE MANUAL
SECTION 0
INTRODUCTION
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Pag. 5
SECTION 1 GENERAL SAFETY ............................................................................................................................. 9
1.1.
GENERAL SAFETY WARNINGS AND SYMBOLS.............................................................................. 11
1.2.
INSTRUMENT USE AND CONDITIONS ............................................................................................... 11
1.3.
INTENDED USE/USERS OF THE INSTRUMENT................................................................................. 11
SECTION 2 INSTALLATION AND TECHNICAL SPECIFICATIONS ........................................................... 13
2.1.
INSTALLATION ...................................................................................................................................... 15
2.2.
WORK AREA............................................................................................................................................ 16
2.3.
TECHNICAL SPECIFICATIONS ............................................................................................................ 16
SECTION 3 MAINTENANCE PROCEDURES .................................................................................................... 17
3.1 SYSTEM ARCHITECTURE ........................................................................................................................ 19
3.2 REMOVING WORK AREA......................................................................................................................... 21
3.2.1. REMOVING WORK AREA .................................................................................................................... 21
3.2.2. Removing shaking plane......................................................................................................................... 23
3.3 DILUTOR UNIT ........................................................................................................................................... 24
3.3.1. DESCRIPTION OF HOW IT WORKS ................................................................................................... 24
3.3.1.1. Dilutor Unit ......................................................................................................................................................24
3.3.1.2. Dilutor mechanism ........................................................................................................................................... 25
3.3.1.3. Valve mechanism/Syringe................................................................................................................................ 25
3.3.1.4. Pump Unit ........................................................................................................................................................26
3.3.1.5. Probe ................................................................................................................................................................ 27
3.3.1.6. Probe Wash Basin ............................................................................................................................................27
3.3.1.7. Dilutor Unit PCB Electrical Diagram...............................................................................................................28
3.3.1.8. Dilutor unit hydraulic component diagram.......................................................................................................29
3.3.2. REGULAR DILUTOR UNIT MAINTENANCE ...................................................................................... 30
3.3.2.1. Visual checks ................................................................................................................................................... 30
3.3.3. SUBSTITUTING DILUTOR UNIT COMPONENTS .............................................................................. 31
3.3.3.1. Substituting valve – syringe – dispensation probe tubing.................................................................................31
3.3.3.2. Substituting valve mechanism..........................................................................................................................32
3.3.3.3. Substituting 3-way Electro valve......................................................................................................................32
3.3.3.4. Substituting 3-way valve mechanism ...............................................................................................................32
3.3.3.5. Substituting Peristaltic Pump Tubing ...............................................................................................................33
3.3.3.6. Substituting Dilutor Motor ...............................................................................................................................34
3.3.3.7. Substituting Opto SIDILOP PCB.....................................................................................................................35
3.3.3.8. Substituting INTERFA2 PCB ..........................................................................................................................35
3.3.3.9. Substituting probe wash basin ..........................................................................................................................35
3.4.
X–Y AXIS ................................................................................................................................................. 36
3.4.1. DESCRIPTION OF HOW IT WORKS ................................................................................................... 36
3.4.1.1. X – Y axis movement mechanism.................................................................................................................... 36
3.4.1.2. X- Y axis Electrical Functions Diagram........................................................................................................... 37
3.4.2. REGULAR MAINTENANCE FOR X–Y AXIS ........................................................................................ 38
3.4.2.1. Cleaning X-Y axis tracks..................................................................................................................................38
3.4.2.2. Check belt tension ............................................................................................................................................ 38
3.4.2.3. Check condition of flat cables ..........................................................................................................................38
3.4.3. CALIBRATING X–Y AXIS SETTINGS ................................................................................................... 38
3.4.3.1. X and Y axis belt tension..................................................................................................................................38
3.4.4. Substituting X–Y AXIS PARTS ............................................................................................................... 39
3.4.4.1. Substituting X-axis Movement Motor.............................................................................................................. 39
3.4.4.2. Substituting Y-axis Movement Probe Motor.................................................................................................... 40
3.4.4.3. Substituting Opto XGOLAV (X-axis movement) PCB.................................................................................... 40
3.4.4.4. Substituting X-axis movement belt ..................................................................................................................41
3.4.4.5. Substituting Flat Cables....................................................................................................................................41
3.4.4.6. Substituting X-Y axis movement flat cables ....................................................................................................41
3.4.4.7. Substituting INTEROP2 PCB (Y-axis movement)...........................................................................................42
3.5.
Z1 - Z2 AXIS ............................................................................................................................................. 43
3.5.1. DESCRIPTION OF HOW IT WORKS ................................................................................................... 43
3.5.1.1. Z1 - Z2 axis movement mechanism..................................................................................................................43
3.5.1.2. Z axis electrical circuit diagram .......................................................................................................................44
3.5.2. REGULAR Z AXIS MAINTENANCE...................................................................................................... 45
3.5.2.1. Z2 axis movement control.................................................................................................................................45
3.5.3. Z-AXIS CALIBRATION .......................................................................................................................... 45
3.5.4. SUBSTITUTING Z1 and Z2 AXIS PARTS ............................................................................................... 46
3.5.4.1. Substituting Z1 Axis probe................................................................................................................................46
3.5.4.2. Substituting Z1 Axis probe motor..................................................................................................................... 47
AP Blot– SERVICE MANUAL
3.5.4.3. Substituting Z2 Axis probe ...............................................................................................................................47
3.5.4.4. Substituting Z2 Axis probe motor..................................................................................................................... 48
3.6.
SHAKING PLANE.................................................................................................................................... 49
3.6.1. DESCRIPTION OF HOW IT WORKS ................................................................................................... 49
3.6.1.1. Shaking plane movement mechanism ..............................................................................................................49
3.6.1.2. Shaking plane circuit diagram ..........................................................................................................................50
3.6.2. MAINTENANCE AND SHAKING PLANE CALIBRATION................................................................... 51
3.6.3. SUBSTITUTING SHAKING PLANE COMPONENTS ........................................................................... 51
3.6.3.1. Substituting Shaking Plane Motor.................................................................................................................... 51
3.6.3.2. Substituting opto PCBs ....................................................................................................................................52
3.7.
CAMERA .................................................................................................................................................. 53
3.7.1. DESCRIPTION OF HOW IT WORKS ................................................................................................... 53
3.7.2. CAMERA MAINTENANCE .................................................................................................................... 54
3.7.2.1. Checking USB cable connection ......................................................................................................................54
3.7.2.2. Cleaning lens....................................................................................................................................................54
3.7.3. CALIBRATE CAMERA........................................................................................................................... 54
3.7.4. SUBSTITUTING CAMERA AND/OR PARTS ........................................................................................ 54
3.7.4.1. Substituting camera ..........................................................................................................................................54
3.7.4.2. Substituting camera lens................................................................................................................................... 55
3.7.4.3. Substituting camera USB cable ........................................................................................................................55
3.7.4.4. Substituting LED light bar................................................................................................................................55
3.8.
OPTICAL SENSORS ................................................................................................................................ 56
3.8.1. DESCRIPTION OF HOW OPTICAL SENSORS FUNCTION ............................................................... 56
3.8.2. REGULAR MAINTENANCE .................................................................................................................. 56
3.8.2.1. Cleaning opto ...................................................................................................................................................56
3.8.2.2. Checking procedure.......................................................................................................................................... 56
3.8.3. CHECKING DISTANCE FOR OPTO COUPLERS (X AXIS) ................................................................ 57
3.8.4. CHECKING DISTANCE FOR OPTO COUPLERS (Y AXIS) ................................................................ 58
3.9.
USB COMMUNICATION ........................................................................................................................ 59
3.9.1. DESCRIPTION OF HOW IT WORKS ................................................................................................... 59
3.9.2. CHECK FUNCTIONALITY ................................................................................................................... 59
3.9.3. REPLACING DILAP21 and SIR4MOT PCBs ........................................................................................ 60
3.10. DILUTOR COMMAND LIST................................................................................................................... 61
3.11. HYDRAULIC CIRCUIT DIAGRAM ....................................................................................................... 66
3.11.1. REGULAR MAINTENANCE .............................................................................................................. 67
3.11.2. SUBSTITUTING HYDRAULIC CIRCUIT COMPONENTS ............................................................... 67
3.11.2.1. Electro-valve substitution.................................................................................................................................67
3.12. SUMMARY OF REGULAR MAINTENANCE ....................................................................................... 68
3.12.1. DILUTOR UNIT ................................................................................................................................. 68
3.12.2. X–Y AXIS ............................................................................................................................................ 68
3.12.3. Z2 AXIS............................................................................................................................................... 68
3.12.4. SHAKING PLANE .............................................................................................................................. 68
3.12.5. CAMERA ............................................................................................................................................ 68
3.12.6. OPTICAL SENSOR............................................................................................................................. 68
3.12.7. HYDRAULIC CIRCUIT ...................................................................................................................... 68
SECTION 0
INTRODUCTION
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Pag. 6
SECTION 4 CALIBRATION PROCEDURES (XCALIB) ................................................................................... 69
4.1.
INSTALLING SOFTWARE ..................................................................................................................... 71
4.2.
OPERATING MENU ................................................................................................................................ 72
4.3.
DESCRIPTION OF MENU FUNCTIONS ................................................................................................ 73
4.4.
INSTRUMENT BAR................................................................................................................................. 74
4.5.
SETTINGS ................................................................................................................................................ 75
4.5.1. HARDWARE CONFIGURATION .......................................................................................................... 75
4.5.2. Language................................................................................................................................................ 76
4.5.3. Communication port............................................................................................................................... 76
4.5.4. Serial number ......................................................................................................................................... 77
4.6.
WORK AREA............................................................................................................................................ 78
4.7.
MAIN FUNCTIONS.................................................................................................................................. 79
4.7.1. Tank level Sensors .................................................................................................................................. 79
4.7.2. Dispensation Test ................................................................................................................................... 79
4.7.3. Dilution Test........................................................................................................................................... 79
4.7.4. Movement Test........................................................................................................................................ 80
4.7.5. Print Preview.......................................................................................................................................... 82
4.7.6. Print........................................................................................................................................................ 82
4.7.7. Turn Light On......................................................................................................................................... 83
4.7.8. Close Function ....................................................................................................................................... 83
AP Blot– SERVICE MANUAL
4.7.9. Exit ......................................................................................................................................................... 83
4.8.
DILUTOR.................................................................................................................................................. 84
4.8.1. Calibrate probe positions ....................................................................................................................... 84
4.8.2. Calibrate probe height ........................................................................................................................... 89
4.8.3. Calibrate LT ........................................................................................................................................... 91
4.8.4. Calibrate shaking plane ......................................................................................................................... 93
4.8.5. Calibrate syringe.................................................................................................................................... 95
4.8.6. Calibrate SUL ........................................................................................................................................ 98
4.8.7. Wash basin test....................................................................................................................................... 99
4.8.8. Prime .................................................................................................................................................... 101
4.8.9. Calibrate camera.................................................................................................................................. 102
4.8.10. Calibrate parameters........................................................................................................................ 104
4.8.11. Home................................................................................................................................................. 105
4.8.12. Turning motors off ............................................................................................................................ 105
4.8.13. Reset.................................................................................................................................................. 105
4.9.
? ............................................................................................................................................................... 106
4.9.1. Instrument information......................................................................................................................... 106
4.9.2. Software component information ......................................................................................................... 107
4.9.3. About .................................................................................................................................................... 107
SECTION 0
INTRODUCTION
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Pag. 7
SECTION 5 TROUBLE SHOOTING ................................................................................................................... 109
5.1 DILUTION / DISPENSATION................................................................................................................... 111
5.2 X - Y - Z MOVEMENTS ............................................................................................................................. 112
5.3 CAMERA.................................................................................................................................................... 112
SECTION 6 SCHEMATIC DIAGRAMS .............................................................................................................. 113
AP Blot– SERVICE MANUAL
GENERAL SAFETY
SECTION 1
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Pag. 9
SECTION 1
GENERAL SAFETY
AP Blot – SERVICE MANUAL
GENERAL SAFETY WARNINGS
SECTION 1
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
1.1. GENERAL SAFETY WARNINGS AND SYMBOLS
In this manual the following symbols stand for danger or warnings:
General DANGER symbol which indicates that a serious safety risk can occur if
instructions and warnings are not followed.
Indicates ELECTRICAL VOLTAGE which could cause death upon contact. Covers
with this symbol can only be removed and replaced by qualified personnel and only
after electrical power has been disconnected.
Indicates that the instrument uses reagents and corrosive, irritating or noxious
DANGEROUS CHEMICAL SUBSTANCES which could damage health.
Indicates that the instrument deals with potentially infectious samples (e.g. body
fluids such as urine) which could cause INFECTION/CONTAMINATION. Always
observe general safety precautions when any of these biological substances are
present.
Indicates that not following the correct instructions could damage instrument and/or
its proper functioning.
Indicates that important information concerning the instrument or a Section of the
document should be read carefully.
1.2. INSTRUMENT USE AND CONDITIONS
The instrument is intended for use in the following working conditions:
− as In Vitro Diagnostic (IVD) medical device as specified in the technical data
− with chemical reagents and accessories supplied and/or declared compatible with instrument
− at a specific temperature and humidity levels as specified in this manual
− not to be used and powered in a potentially explosive or fire hazardous environment
This instrument should only be used as described in this manual.
Any other use has to be regarded as improper.
1.3. INTENDED USE/USERS OF THE INSTRUMENT
The instrument should only be used for the intended purpose and in perfect technical conditions, only by
qualified personnel following strict safety procedures and regulations for accident prevention.
This manual contains instructions for qualified personnel:
− Only Qualified Technicians are entitled to service and repair the instrument with original spare
parts and after appropriate training.
Modifications of the instrument are not allowed. The user is liable for any improper
modifications and any subsequent consequences.
For extraordinary maintenance - request Specialized Technicians with authorised equipment and
original spare parts from authorised service centers.
Pag. 11
AP Blot – SERVICE MANUAL
INSTALLATION AND TECHNICAL SPECIFICATIONS
SECTION 2
Doc. N° MDS-AP-26-00-02
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Date: 02.04.2009
Pag. 13
SECTION 2
INSTALLATION AND TECHNICAL SPECIFICATIONS
AP Blot – SERVICE MANUAL
7
10
8
12
9
11
2
3
4
5
6
1
INSTALLATION AND TECHNICAL SPECIFICATIONS
SECTION 2
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Pag. 15
2.1. INSTALLATION
The instrument is packed with a wooden box. To unpack the instrument follow the instructions described below:
a) Remove the 2 plastic covers (1-2) from the instrument right side and the other ones from the left side. Screw
in the 4 handles on both sides (3-4) for manual instrument placement. Once in position remove the handles e
put back in place the plastic covers.
b) Remove the 4 screws that hold the instrument on to the wood base (5-6)
c) Remove screw and bracket (7), screw in the same place using the screw removed before
d) Remove,.- screw and bracket (7), screw in the same place the enclosed screw in the bag
e) Remove screws (8,9,10) and related brackets
f) Remove the Z block holder (11) as indicated
g) Remove the protection cap from the camera (12)
h) Remove the blocking tools (13) from the oscillating plane
2.2. WORK AREA
AP-Blot work area showing the various components:
Probe washing
basin
48 position Sample rack Reagent and control rack Tanks
AP Blot – SERVICE MANUAL
SECTION 2
INSTALLATION AND TECHNICAL SPECIFICATIONS
Doc. N° MDS-AP-26-00-02
Rev.01
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Pag. 16
Camera Calibration area Strip holder rack
Fig. 1 - AP-Blot Work Area
2.3. TECHNICAL SPECIFICATIONS
Sample rack
Reagent rack
Liquid Containers
Strip Holders
Dispensation System
Strip Washing
Image acquisition system
Dispensation
Software
Minimum PC Requirements
Power Requirements
Optional
Measurements
Weight
48 sample tubes (Ø 13mm)
6 x 100ml vials and 8 control positions
2 tanks for wash buffer and waste. Both with liquid level sensors
6 slides (8 strips per slide) to process up to strips on oscillating plane
1 dispensation and 1 aspiration probe
Liquid dispensation and aspiration via two instrument probes
High Resolution Colour Camera (3.3 Megapixels) mounted above Probes
Up to 2500 µl with a 1 µl resolution
Windows 2000 and XP compatible to set up work list, reports and result archive.
Loading work lists and transmit results via Host Computer interface (LIS)
− 200 MB free hard disk space
− CD-Rom reader
− RAM 512 MB
− Pentium 4
− Standard video card:VGA 1024x768 16 million colours
− Free USB 2.0 port
230/115 VAC, 50-60Hz, 150W
Bar Code Reader (BCR) for samples with position ID
79 x 61 x 65 h (in cm)
71 Kg
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Page 17
SECTION 3
MAINTENANCE PROCEDURES
CAMERA
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
3.1 SYSTEM ARCHITECTURE
The system architecture is illustrated in the following diagram and described in table below:
Function group
Page 19
Control and check board
Functional unit Description
X-Y AXIS
Z1, Z2 AXIS
DILAP21
USB
DILUTOR
UNIT
SHAKING PLANE
Personal
Computer
X – Y Axis
Z1 Axis
Z2 Axis
Dilutor unit
Shaking Plane
Camera
The X-Y axis movement mechanism moves probe arms over work area with precision
The Z1 axis raises and lowers dispensing probe over work area.
The Z2 axis raises and lowers aspiration probe over work area.
The dilutor unit aspirates and dispenses samples, calibrators, controls, and generally all
reagents. It also washes Z1 axis probe internally and externally.
The shaking plane shakes the 6 slides holding the strips to be processed
The camera acquires the images of the processed strips.
The AP Blot communicates with the PC via a serial connection: between the Dilutor PCB and the PC.
The units controlled by the PCB are:
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
PCB Unit
X – Y Axis
Z1 – Z2 Axis
DILAP21
Dilutor unit
Shaking plane
Doc. N° MDS-AP-26-00-02
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Page 20
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
3.2 REMOVING WORK AREA
3.2.1. REMOVING WORK AREA
a) Turn off instrument
b) To remove strip holder tray plate first loosen four screws holding shaking plane
4 fixing
screws
Page 21
c) Remove 2 fixing screws from Probe wash basin and detach Waste (“OUT”) tube from under basin
2 fixing
screws
d) Remove connector from shaking plane positioning opto PCB (OP-Swing PCB)
1 connector
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
Doc. N° MDS-AP-26-00-02
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Page 22
e) Remove 8 screws from bottle separation carter
f) Remove 7 screws from aluminium shaking plane
Bottle separation
carter
Aluminium
shaking plane
g) Lift up and detach screws holding the shaking plane movement mechanism motor
h) NB: each time shaking plane support is moved, or removed calibrate shaking plane as described Para. 4.8.4.
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
3.2.2. Removing shaking plane
a) Turn off device
b) Remove 8 screws from strip holder tray plate.
8 fixing
screws
c) Remove strip holder tray plate
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
strip holder
tray plate
Page 23
d) Lift up shaking plane
Shaking
plane
OP-Swing
PCB
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
3.3 DILUTOR UNIT
3.3.1. DESCRIPTION OF HOW IT WORKS
FUNCTION
The dilutor unit aspirates and dispenses samples, calibrators, controls and all reagents, and also cleans
inside of Z1 probe.
3.3.1.1. Dilutor Unit
Doc. N° MDS-AP-26-00-02
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Page 24
SIDILOP
PCB
Syringe
Dilutor
movement
mechanism
Valve support
INTERFA2
PCB
Electro-valve
The DILUTOR UNIT is composed of following parts (clockwise direction):
1 Dilutor mechanism
2
3 Syringe
2-way Electro-valve For the aspiration of washing solution or for probe aspiration/dispensation
Moves probe with step motor
To aspirate and dispense
Dilutor unit
4
5
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
Doc. N° MDS-AP-26-00-02
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Page 25
3.3.1.2. Dilutor mechanism
This mechanism moves the syringe plunger (1) up and down in small steps. The movement is generated by a step
motor (2) which moves a special worm screw (3) inside a female plastic nut 4) fixed on a carriage (5) which runs along
a track (6) on 4 ball bearings. A shaft is mounted on the carriage (7) which then moves the syringe plunger (1). The
optic sensor (8) is mounted on the SIDILOP PCB and signals when the syringe plunger has reached the End line
(“home”) position.
4
6
1
3
8
7
3
2
2
3.3.1.3. Valve mechanism/Syringe
The valve mechanism connects the Wash bottle to the rest of the hydraulic syringe-probe circuit through the peristaltic
pump.
The syringe movement is generated by the dilutor unit and the syringe is connected to the dispensing probe
MAINTENANCE PROCEDURES
3.3.1.4. Pump Unit
AP Blot - SERVICE MANUAL
SECTION 3
Doc. N° MDS-AP-26-00-02
Rev.01
Date: 02.04.2009
Peristaltic
pump A
Page 26
Peristaltic pump
tubing
Peristaltic
pump B
Peristaltic pump
tubing
The pump unit is made up of 2 peristaltic pumps composed of:
- rotating wheel
- stand
- peristaltic pump tubing
- step motor
Note:
• Pump A washes dispensation probe internally and externally and regulates WASH liquid flow during STRIP
WASH phase.
Groud
Liquid level
Microprocessor
Impulse duration T
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
3.3.1.5. Probe
The probe has two functions:
• Liquid aspiration and dispensation
• Liquid sensor (for liquid level)
Aspiration and dispensation
The probe aspirates and dispenses controls, samples, etc.
Doc. N° MDS-AP-26-00-02
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Page 27
Aspiration
Probe
Liquid Sensor
The probe is fitted with a liquid sensor which regulates
its movements near the liquid to be aspired.
This sensor reads the variations of the electrical capacity
of the circuit which consists of probe – liquid – ground.
The sensor circuit emits a low signal (level 0) until the
probe touches the liquid.
A high signal (level 1) is emitted to indicate when the
probe touches the liquid for duration ‘T’ in proportion to
the variation of the circuit capacity.
Adjusting probe sensitivity is based on the
duration/bandwidth of this impulse. (Below a certain
value this impulse is only considered noise and not valid
as a liquid surface signal).
sensor
Probe
3.3.1.6. Probe Wash Basin
The probe wash basin is used for external probe washing and to dispose of liquid excess.
Dilutor unit
Probe washing
basin
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
Doc. N° MDS-AP-26-00-02
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Page 28
3.3.1.7. Dilutor Unit PCB Electrical Diagram
The table below describes each PCB and the various dilutor unit components they control and the diagram shows the
PCBs and the dilutor unit electrical circuit.
DILAP21 (Dilutor) PCB a micro-processor, a memory and electro-valve driver
SIR4MOT PCB syringe motor driver.
SIDILOP PCB optical sensor which senses when syringe has reached end line (“home”) position.
INTEROP2 PCB Support for liquid level sensor and cable distribution
INTERFA2 PCB Connects wire cables
Liquid Level Sensor Electronic circuit in a small container mounted on probe which signals contact with liquid surface
Syringe Motor A step motor which moves syringe plunger (with special “worm” screw inside the plastic nut).
Dilutor 2-way Electro-valve Manages washing solution aspiration or probe aspiration/dispensation.
(level)
Memory
Microprocessor
DILAP21 PCB
INTEROP2
PCB
Liquid Level
Sensor
Syringe
motor
driver
SIR4MOT
PCB
INTERFA2
PCB
2 way
electrovalve
SIDILOP
PCB
M
Syringe motor
Dis pens ation probe
Aspir ation pr obe
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
Doc. N° MDS-AP-26-00-02
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3.3.1.8. Dilutor unit hydraulic component diagram
The table below describes the different functions of the dilutor unit hydraulic components.
The diagram shows how the dilutor unit circuit is set up to work.
WASH bottle Contains probe-washing liquid, 5 litre capacity
Level Sensor Sensor for the three different bottles and connecting cables and connectors
Probe washing pump Peristaltic pump A for washing probe, internally and externally, driven by step motor
Probe wash basin For external probe wash and draining any excess liquid
Syringe For aspirating and dispensing liquid through probe
Page 29
3 way
Electro valve
Syringe
Needle washing basin
3 way E lectrovalve
Peristaltic P um p A
Peristaltic P ump B
WA SH
WA SH BU FFE R
WA STE
WA STE bottle
WA SH BU FFE R
bottle
WA SH bottle
AP Blot - SERVICE MANUAL
SECTION 3
MAINTENANCE PROCEDURES
3.3.2. REGULAR DILUTOR UNIT MAINTENANCE
Visually check the following components:
− Tubing connecting Valve – Probe.
− Syringe plunger
Doc. N° MDS-AP-26-00-02
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Page 30
1
3.3.2.1. Visual checks
• Tubing connecting Valve – Probe:
Check that there is no narrowing or kinks or obstructions on any of this tubing.
If these faults are found the tubing can be substituted as described in para. 3.3.3.1.
• Syringe plunger:
Check that the syringe plunger and tip are in good condition (they should be clean and without any liquid dripping
from syringe bottom).
If condition is unsatisfactory, substitute plunger following procedure described in para. 4.8.5.
• Syringe:
Check that syringe glass is in good condition. If any damage can be seen then substitute syringe following procedure
described in paragraph 4.8.5.
• Peristaltic pump tubing:
Check that tubing is in good condition and more specifically check that there are no traces of rubber around pump
area. If condition or area is unsatisfactory change this rubber tubing as described in para. 3.3.3.5.
• Shaking plane:
Check that shaking movement is smooth, with no obstructions or hesitations. If movement is unsatisfactory check
shaking mechanism as described in Para. 3.6.1.1.
• Camera Calibration strip:
Check that camera calibration strip is clean and intact. If condition is unsatisfactory, clean off strip with a clean cloth
and/or substitute strip.
• Camera:
Check that electrical cable is correctly plugged in
− Syringe
Every 4 months Ref. para. 3.3.2.1.
− Peristaltic Pump tubing
− Shaking plane
− Calibration Strip
− Camera
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