ChemoMetec YC-100 User Manual
NucleoCounter
User's Guide
Revision 1.2
YC-100
Technology that counts
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NucleoCounter YC-100
Yeast Cell Counter
P /N 9 9 1- 0 3 0 0 ( E n gl i s h )
R ev i s i on 1 . 2
D ec e m b er , 2 00 5
ChemoMetec A/S
Gydevang 43, DK-3450 A llerød, Denmark
Telephone: (+45) 48 13 10 20, Fax: (+45) 48 13 10 21
Internet: www.chemometec.com,
E-mail: info@chemometec.com
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Caution!
Caution!
Caution!Caution!
This equipment mu st b e op erated as des cribed in this Us er’ s Gu id e. P le ase read the enti re guide before
attempting to use thi s un it . Please pa y at te ntion to that gloves or protective cloth in g are not worn on the
illustrations/p ic tu res shown in this U ser’s Guide. However, Chemo Me te c A/S does recommend that the user
wear suitable protect iv e clothing etc.
Contacting support
Contacting support
Contacting supportContacting support
Technical i nfor ma ti on including product li te rature, answers t o qu estions regarding t he operation of the
NucleoCounter YC-100™ not co ve re d in this document and in formation on soft wa re u pg rade is available
through the follo wi ng :
• For e-mail support, s end qu estions to Nu cl eo Co un ter YC-100™ Tech nical Support on the address
sup
sup p ort @ch emo met e c
por t@c hem ome t ec.com
supsup
por t@c hem ome t ecpor t@c hem ome t ec
• To speak with a T ec hnical Support Speciali st , call (+45) 48 13 10 2 0.
Please note the Nuc le oC ou nt er YC-100™ se rial number and have it a va il ab le w hen contactin g Ch em oM etec
A/S for support. Th e Nu cleoCounter YC-100™ serial n um be r is found on the label a ffixed to the bottom of the
instrument and in t he display upon sta rt -u p.
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Sales and ordering information
Sales and ordering information
Sales and ordering informationSales and ordering information
For sales assis ta nce with NucleoCounter YC-100™ or the N uc leoView so ftware , to place an order f or a
NucleoCounter YC-100™ or con su ma bl es, call (+45 ) 48 13 10 20, fax (+45 ) 48 13 10 21, or sen d e- mail t o
sal es@ che mom etec
sal es@ che mom etec .co m
sal es@ che mom etecsal es@ che mom etec
Disclaimer Notices
Disclaimer Notices
Disclaimer NoticesDisclaimer Notices
The material in t hi s document is for inf ormation only and is su bj ec t to change without not ic e. While reason ab le
efforts have been m ad e in t he preparat io n of this document to assure its accuracy, ChemoMete c A/S assumes
no liability resultin g from errors or omission s in this document, or from the use of the inf ormation contained
herein.
ChemoMetec A/S rese rv es the right to make changes in the pr od uc t design without rese rv at io n and without
notification to i ts u sers.
Copyright Notices
Copyright Notices
Copyright NoticesCopyright Notices
Co py right © ChemoMete c A/S 2005. All r ig hts reserved. No part o f this publication may be rep roduced, stored
in a retrieval sy st em , or transmitted in any f or m or by any means, electronic, m ec ha ni cal, photocopying,
recording or othe rw ise, without the prio r wr it ten consent o f Ch em oMet ec A/S, Gydevang 43, DK -3450 Aller ød ,
Denmark.
.c o m
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ChemoMetec and Nucleo Co un ter are reg is tered trademarks ow ne d by C hemoMetec A/S. Nu cl eo Counter ,
NucleoCassettes , Re agent Y100 and Nucleo Vi ew a re trademarks o f ChemoMetec A/S.
All other tra de ma rk s are the property of the ir respective owners.
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Declaration of Conformity
Name of product:
Type:
YC-100
Other identifying data:
NucleoCounter
Part no. 900-0300
The product complies with requirements of the following directives:
89/336/EEC - Electromagnetic Compatibility (EMC)
89/392/EEC - Machinery Directive
Harmonized standards, which have been used:
EN61326: 1997+A1: 1998 (Class B) +A2: 2000, Electrical equipment for measurement,
control and laboratory use – EMC requirements (emission and immunity). Annex B and C
from A1: 1998 is used.
This device complies with Part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) this device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause undesired
operation.
Date: 2005-11-04 Signed:
Name: Frans Ravn
Position: Chief Technology Officer
Name and address of manufacturer:
ChemoMetec A/S
Gydevang 43
DK-3450 Allerod
DENMARK
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WEEE directive information – Europe only
Correct Disposal of This Product
(Waste Electrical & Electronic Equipment) - Europe only
This marking shown on the product or its literature, indicates that it
should not be disposed t ogether with other household wastes at the end
of its working life. To prevent possible harm to the environment or human
health from uncontrolled waste disposal, please separate this from other
types of wastes and recycle it responsibly to promote the sustainable
reuse of material resources.
Business users should contact their supplier and check the terms and
conditions of the purchase contract. This product should not be mixed
with other commercial wastes for disposal.
This information is listed in “Appendix 1: WEEE directive information in
more EU languages”.
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ix
Introduction and intended use
Introduction and intended use
The NucleoCounter YC-100 is intended to be used for the determination of yeast cell
concentration in sample containing dispersed yeast cells in suspension.
The NucleoCounter YC-100 has been developed
to count dispersed yeast cells in suspension
The instrument is a part of the NucleoCounter YC-100 system, which also comprises a
disposable NucleoCassette and a lysis buffer (Reagent Y100).
The NucleoCounter YC-100 system allows reliable, fast and objective cell counting to be
carried out based on an automated method of fluorescence micr oscopy. The system
enables the user to stain and count cells with good protection ag ainst being exposed to
potentially hazardous DNA staining dyes.
The NucleoCounter YC-100 is developed as a stand-alone instrument but optionally it
can be connected to a computer via an USB-interface. When connected to a computer
the NucleoView software offers various features such as documentation of the obtained
results. For further description of the software please refer to the NucleoView User’s
Guide.
The NucleoCounter YC-100 has the ChemoMetec part number 900-0300. Refer to section
10.1 with respect to other part numbers of the items of the NucleoC ounter YC-100
system.
The NucleoCounter YC-100 system is not intended for human or veterinary diagnostic
purposes.
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Introduction and intended use
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xi
Warnings and precautions
Wherever the symbol appears on the NucleoCounter instrument, it indicates that the
manual must be consult ed for precautions and warnings.
Power and cables
Use the shielded USB cable supplied with the NucleoCounter to ensure that you maintain
the appropriate EMI classification for the intended environment.
The USB interface connector of the NucleoCounter must only be connected to SELV
circuits. External computing devices connected to the USB interface connector of the
NucleoCounter has to comply with the standards, UL 1950 and IEC/EN 60950.
Table
Table 1111.
Table Table
Description of the NucleoCounter USB interface connector
Use the shielded printer cable supplied with the NucleoCounter to ensure that you
maintain the appropriate EMI classification for the intended environment.
The printer interface connector of the NucleoCounter must only be connected to the
printer supplied by ChemoMetec A/S. External computing devices connected to the
printer interface connector of the NucleoCounter has to comply with the standards, UL
1950 and IEC/EN 60950.
Pin no. Name
1 +5V +5 VDC
2 D- +3.5 VDC
3 D+ +3.5 VDC
4 DGND 0 VDC
Metal
enclosure
Shield
(connected
to DGND)
Maximum
Voltage level
0 VDC
1
1
In normal operation mode (refers to Pin no 4)
1
Tab le
Tab le 2222 Description of the NucleoCount er Printer Output connector
Tab le Tab le
2222
Pin no
Pin no Nam e
Pin noPin no
Nam e Max imum Volt age level
Nam eNam e
Max imum Volt age level
Max imum Volt age levelMax imum Volt age level
13
2 Rx
±10 VDC
3 Tx ±10 VDC
Metal Enclosure DGND 0 VDC
3
1
2
Metal
Enclosure
Figure
Figure 1111 Printer Output connector seen from the cable entry side.
Figure Figure
NucleoCounter is powered by an external 12VDC power supply. For safe use, please
follow the instructions for connecting the power supply.
The NucleoCounter shall only be used with one of the following external power
supplies:
• Power supply Class I, Proton Electronic Industrial Co. Ltd., model SPN-260-12,
input rated 100-240 Vac, 50-60Hz, 1.6 A. Output rated 12 Vdc, 4.3 A.
• Power Supply Class I, Powerbox Europe AB, model EBH 03 131, input r ated 100-
240 Vac, 47-63 Hz, 0.8A. Output rated 11-13 Vdc, 2.7 A.
• Power supply Class II, Celetron USA Inc., model ZVC40LT12E, input rated 100-240
Vac, 50-60Hz, 1.2 A. Output rated 12 Vdc, 3.3 A.
The detachable power supply cord set and appliance inlet of the external power
supply are considered as the disconnecting device.
The mains supply cord and plug of the external power supply shall comply with any
national regulations.
2
In normal operation. Refers to DGND Metal Enclosure. Taken from the datasheet for
MAX202ECSE.
3
There is no signal connected to pin 1.
2
The user shall be made aware of that, if the N ucleoCounter and the external power
supply is used in a manner not specified by the manufacturer, the protection provided
by the NucleoCounter and the external power supply may be impaired.
Electromagnetic interference
NOTE: This equipment has been tested and found to comply with the limits for a Class B
digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide
reasonable protection against harmful interference in a residential installation. This
equipment generates, uses and can radiate radio frequency energy and, if not installed
and used in accordance with the instructions, may cause harmful interference to radio
communications. However, there is no guarantee that interference will not occur in a
particular installation. If this equipment does cause harmful interference to radio or
television reception, which can be determined by turning the equipment off and on, the
user is encouraged to try to correct the interference by one or more of the following
measures:
• Reorient or relocate the receiving antenna.
• Increase the separation between the equipment and receiver.
• Connect the equipment to an outlet on a circuit different from that to which the
receiver is connected.
• Consult the dealer or an experienced radio/TV technician for help.
Caution! Changes or modifications not expressly approved by the party responsible for
compliance could void the user's authority to operate the equipment.
Cassettes, Reagents and Dispensers
With respect to usage and handling of cassettes, lysis buffers and dispensers, please
refer to appropriate package inserts for these items.
Caution! When using a bottle-top dispenser: To protect against accidental splashes
protective clothing, eye protection and gloves must be worn when using potentially
hazardous liquids.
General
Any biological specimen should be handled as if it is capable of transmitting infectious
disease and disposed of with proper precautions according with federal, state and local
regulations.
Avoid specimen contact with skin or mucous membranes.
3
Never pipette by mouth.
Avoid cross contamination of the samples when preparing of the samples. This can
compromise the quality of the results.
4
Table of contents
DECLARATION OF CONFORMITY ................................................................................ VI
WEEE DIRECTIVE INFORMATION – EUROPE ONLY ...................................................... VIII
INTRODUCTION AND I NTENDED USE .......................................................................... X
WARNINGS AND PRECAUTIONS ................................................................................. 1
1 INSPECTION AND UNPACKING OF EQUIPMENT ...................................................... 7
2 INTRODUCING THE NUCLEOCOUNTER YC-100 ........................................................ 9
2.1 T
2.2 T
2.3 L
3 CONTROL BUTTONS, KEYPAD AND DISPLAY ......................................................... 15
3.1 I
3.2 I
HE NUCLEOCOUNTER
2.1.1 Fluorescence microscope ........................................................................... 10
2.1.2 Built-in image analysis .............................................................................. 11
HE NUCLEOCASSETTE
2.2.1 Loading the cassette .................................................................................. 12
2.2.2 Fluorescent dye ......................................................................................... 12
2.2.3 Sample volume .......................................................................................... 12
2.2.4 Cassette handling ...................................................................................... 13
YSIS BUFFER
NTERACTIVE CONTROLS
3.1.1 Control Buttons .......................................................................................... 15
3.1.2 Keypad ...................................................................................................... 15
NTERFACE DISPLAY
3.2.1 Status indicator ......................................................................................... 16
3.2.2 Display screen ........................................................................................... 16
............................................................................................... 13
YC-100
..................................................................................... 11
........................................................................................ 16
INSTRUMENT
................................................................................... 15
............................................................ 9
4 INSTALLATION AND START-UP ........................................................................... 17
4.1 P
4.2 I
5 OPERATION OF THE NUCLEOCOUNTER YC-100 ...................................................... 19
5.1 R
5.2 I
5.3 M
5.4 R
OWER ON, POWER OFF
4.1.1 Starting up ................................................................................................. 17
4.1.2 Shutting down ........................................................................................... 18
NSTALLATION O F NUCLEOVIEW
EADY MODE
NSERTING AND REMOVI NG T HE CASSETTE
5.2.1 Protection of the optical system ................................................................ 19
5.2.2 Inserting cassette ...................................................................................... 19
EASURING - THE RUN BUTTON
5.3.1 Actuator movements ................................................................................. 20
5.3.2 Result presentation ................................................................................... 20
5.3.3 External computer ..................................................................................... 21
ESULT DISPLAY
................................................................................................ 19
................................................................................... 17
.......................................................................... 18
............................................................... 19
......................................................................... 20
............................................................................................ 21
5
5.4.1 Result mode .............................................................................................. 21
5.4.2 Saving parameters ..................................................................................... 22
5.4.3 Setting Date (F200) and Time (F201) ......................................................... 22
5.4.4 Reset Counter (F30) ................................................................................... 22
5.4.5 LCD contrast (F220) ................................................................................... 22
5.4.6 Instrument Info (F100) .............................................................................. 23
5.5 I
5.6 Z
6 TOTAL AND VIABILITY COUNT ............................................................................ 27
6.1 NON-
6.2 T
6.3 D
7 MAINTENANCE OF NUCLEOCOUNTER YC-100 ........................................................ 29
7.1 C
NSTRUMENT SENSITIVITY
ER O COUNT CHECK
VIABLE CELL CONCENTRATION
OTAL CELL CONCENTRATION
ETERMINATION OF VIABILITY
LEANING
.................................................................................................... 29
(F11) ........................................................................ 23
(F50) ............................................................................... 24
..................................................................... 27
............................................................................. 27
........................................................................... 28
7.1.1 Cassette insertion area .............................................................................. 29
7.1.2 Optical elements ....................................................................................... 29
7.1.3 Removal of dust particles .......................................................................... 30
7.1.4 Spill of liquid ............................................................................................. 31
8 TROUBLESHOOTING - ERROR MESSAGES ............................................................. 33
8.1 N
8.2 A
8.3 A
8.4 S
8.5 S
8.6 P
9 TECHNICAL SPECIFICATIONS .............................................................................. 37
9.1 T
9.2 T
9.3 EMC/EMI
10 EQUIPMENT AND ACCESSORIES ....................................................................... 38
10.1 E
10.2 P
10.3 M
10.4 D
10.5 E
O VALID CASSETT E
NALYSIS ABORTED
CTUATOR ERROR MESSAGES
AMPLE COULD NOT B E AN ALYZED
ENSOR ERROR
OWER-ON FAILURE
ECHNICAL SPECIFIC ATIONS FOR NUCLEOCOUNTER
HE NUCLEOCASSETTE
S TANDARDS
QUIPMENT AND ACCESSORIES LIST
OWER SUPPLY
AINS POWER CORD
RY COM PRESSED AIR
XTERNAL PRINTER (OPTIONAL
........................................................................................ 33
........................................................................................ 33
............................................................................. 34
....................................................................... 34
............................................................................................. 34
........................................................................................ 35
™ ................................................................................... 38
................................................................................... 38
.................................................................. 38
.......................................................................................... 40
................................................................................... 40
.................................................................................. 41
) ...................................................................... 42
YC-100 ......................................... 37
11 APPENDIX 1: WEEE DIRECTIVE INFORMATION IN MORE EU LANGUAGES ............... 45
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