ChemoMetec NucleoCounter NC-250 User Manual

NucleoCounter® NC-250™

Instrument User's Guide

P/N 991-0251

Revision 1.3

991-025 1 Rev. 1.3 June-2 016

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NucleoCounter



NC-250™

Flexible Cell Counter

P / N 9 9 1 - 0 2 51 (E n g l i s h )

R e vi s i o n 1 . 3

J u ne , 2 0 1 6

ChemoMetec A/S

Gydevang 43, DK-3450 Allerod, Denmark

Telephone: [phone], Fax: [phone]

Intern et: www.chemometec.co m,

E-mail: [e-mail] m

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Caution!

This equ ip ment must b e operated as d es cr ib ed in this U se r’ s Gui de a nd documents re ferred to h erei n. Pl ea se
read the e ntire guide and refe rr ed d ocuments before atte mp ti ng to use t hi s unit. Ple as e pay attention t o that
gloves o r protective cl othing are no t worn on the ill us trations/pi ctur es shown in this User ’s Guide. How ever ,
Chem oM etec A /S does recom me nd that the u se r wear su itab le protective c lothing etc.

Contacting support

Techni ca l information i nclu ding produc t literature, a ns wers to q ue st ions regarding the operation of th e
NucleoCo un ter NC- 250™ no t covered i n this document and re fe rred doc uments is availabl e through the
followin g:

 For e- ma il support, s en d questio ns t o Nu cleoCount er



NC- 250™ T echn ical Suppor t on the addr es s

Suppor t@ ch em omet ec.com

 Check ou t the FAQ secti on under su pp or t at www.chemo me tec. com
 To speak w ith a Techn ic al S upport Specia li st, call (+ 45 ) 48 13 10 20 .

Please n ot e the NucleoC ou nter NC- 250™ ser ia l number an d ha ve it availab le when contact in g ChemoMetec
A/ S fo r su pport. Th e Nu cleo Coun ter NC- 250™ s er ial number is f ound on t he label af fi xe d to the rear of the
instrume nt . The versi on num ber of th e Nuc le oV iew NC-250™ sof tw are shall als o be noted, thi s ca n be foun d on
the He lp – About me nu item in th e Nucle oV iew NC-250™ sof tw ar e.

Sales and ordering information

For sa le s assista nc e with Nucleo Counte r NC- 250™ o r th e Nucle oV ie w NC-250™ s of twar e, to pla ce a n order for
a Nucl eoCo unter NC- 250™ o r consumables , ca ll ([phone], fax (+ 45) 48 13 10 21 , or send e -mail to
Sales@ch em ometec .com

Disclaimer Notices

The ma te ri al in thi s document and referre d do cuments i s for informa ti on o nly and is su bject to ch an ge without
notice. Wh ile reasona bl e effo rts have be en ma de in prepa ra tion of these document s to assure the ir accuracy ,
Chem oM etec A /S assumes no l iability re su lt ing from erro r s or om is si on s in these documents, or from the u se
of the i nformation cont ai ned herein.

Chem oM etec A /S reserves t he right to m ake changes in th e product d es ign without r es ervation an d wi thout
notifi ca ti on to its u se rs.

Copyright Notices

Copyrigh t © ChemoMe te c A/S 2 010. All rights re se rv ed . No part o f th is public at ion a nd referred d oc um ents
ma y be r ep roduced, st or ed in a r et ri ev al system , or tra nsmitted in any form o r by any means, e lectronic,
mechanic al , photocopying, r ecording or o ther wise, w itho ut the pr ior w ritten cons en t of ChemoM etec A /S,
Gydevang 4 3, DK-3 45 0 Alle rod, De nm ar k.

Chem oM etec a nd NucleoCoun ter are registe re d trademarks ow ned by Chem oM et ec A/S. Nucle oC ount er,
Nucleo Ca ss ette, NC- sl ide and Nuc le oV iew are tradema rk s of Chem oM etec A /S.

All ot he r trademarks ar e the pro pe rt y of their re sp ective owners.

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Declaration of Conformity

991-025 1 Rev. 1.3 June-2 016 vii

Declaration of Conformity

Name of product: NucleoCounter



NC-250™

Type: Flexible Cell Counter
Other identifying data: Part no. 900-0251

We declare under our sole responsibility that the products, to which this declaration
relate s, are in confor mity with the Council Directives on the approximation of the laws of
the EEC Membe r States relating to the following:

Directives

Electromagnet ic Compatibility (2 004/108 /EC). EMC
Low Voltage Directive (2006/95/EC) LVD
Machin ery D irective (2006 /42/EC) MD

Standards
EMC:

EN 61326-1:2006 Electrical equip ment for laboratory use
EN 61000-3-2: 2006 Limits for harmonic current emissions
EN 61000-3-3: 1995 +A1 +A2:2005 Limitation of voltage changes, voltage
fluctuations and flick er

Standards
LVD:

EN 61010-1:2001 Safety requirements for electrical equipment for
measur ement, control, and laboratory use

Standards
MD:

EN 12100-1: 2003 Safety o f machinery; Basic co ncepts, general principl es
for design — Part 1
EN 12100-2: 2003 Safety o f machinery; Basic conc epts, g eneral principles
for design — Part 2:
EN ISO 14121-1:2007 Safet y of ma chinery; risk assessment

This device complies with Part 15 of the FCC Rules. Ope ration is subj ect to the following
two condition s: (1) this devi ce may not cause harmful inter ference, and (2) this device
must accept any interference received, including int erferen ce that may cause undesired
operation.

Date: 2012-Sept-3rd Signed:

Name: Sarah R. Rasmu ssen

Position: QA/QC Managers

Name and address of manufacturer:

ChemoM etec A/S
Gydevang 43
DK-3450 Allerod
DENMAR K
www.chemometec.com

Declaration of Conformity

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WEEE directive information – Europe only

991-025 1 Rev. 1.3 June-2 016 ix

WEEE directive information – Europe only

Correct Disposal of This Product
(Waste Electrical & Electronic Equipment) - Europe only

This marking sh own on the product or its literature, indicates that it should
not be disposed togeth er with other household wastes at the end of it s
working life. To prevent possibl e harm to the en vironment or human health
from uncontro lled waste disposal, pl ease separate this from other types of
wastes and recycle it responsibly to promote the sustainable reu se of
material resources.

Busine ss user s should contact their supplier and check the terms and
conditions of the purchase contract. This p roduct should not be mixed with
other commerc ial wastes for disposal.

This information is listed in “Appendix B: WEEE directive infor mation in
more EU languages”.

WEEE directive information – Europe only

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Introduction and intended use

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Introduction and intended use

The Nu cleoCounter® NC-250™ is a co mpact cell -analyzer intended to be used for advanced
cell analysis within the life-science and pharmaceutical markets. The NucleoCounter® NC­250™ system is intended for research use only, not for diagnostic use. Th e NucleoCounter®
NC-250™ uses special designed slides with 2 or 8 chambers. This will cover an analysis
volume from 0.8µl to 16µl.

The Nu cleoCounter® NC-250™ is a highly advanced cell analyzer based on fluor escence
microscopy using d igital camera technology with low magnification an d advanced image
analysis. Num erous properties of the individual cel ls can be analyz ed with the solid-state
integrated triple-light sou rce (365 nm, 50 0 nm and a white dark field light source) and with
a dual-band emission filter. Spatial position is main tained from wavelength to wavelength
enabling direct comparison of the different parameters detected or quantified on each
individual cell. The l ow magnification (x2) guarantees a high analysis volume an d thus
ensure s a high stati stical performance.

Specially designed sample illumination eliminates the risk of bleaching of adjacent sampl e
material and therefore several images can be exposed in the same sa mple window. Since
no tubes, valves, ho ses etc. are present th e instrument is virtually service and
maintenance free.

The Nu cleoCounter® NC-250™ part number at Ch emoMetec A/S is 900 -0251.

Introduction and intended use

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Warnings and precaut ions

991-025 1 Rev. 1.3 June-2 016 xiii

Warnings and precautions

Whenever the symbo l appears on the NucleoC ounter® NC-250™ instrument, it
indicates that the manual must be consulted for pr ecautions and warnings.

Power and cables

Use th e shielded USB cable supplied with the Nu cleoCounter® NC-250™ to ensure that
appropriate EMI classification is maintain ed for the intended environment.

The USB interface connector of the NucleoCounter® NC-250™ must only be connected

to SELV circuits. External computing devi ces connected to the USB int erface connector of
the NucleoCounter® NC-250™ has to co mply with the standards, UL 1950 and IEC/EN

60950.

Table 1. Description of th e NucleoCounter® USB interface connector

NucleoCounter® NC-250™ is powered by an ext ernal 24VDC power supply . For safe u se,
please follow the inst ructions for connecting the power supply.

The NucleoCounter® NC-250™ shall only be used with an external power supply

purchased at ChemoMetec A/S.

The d etachable DC power supply cord set and applia nce inlet of the external power

supply are considered as the disconnecting devic e.

The mains supply cord and plug of the external power supply sha ll comp ly with any

national regula tions.

1

In normal operation mode (refers to Pin no 4)

Pin no.

Name

Maximum

Voltage level1

1

+5V

+5

VDC 2 D-

+3.5

VDC 3 D+

+3.5

VDC 4 DGND

0

VDC

Metal

enclosure

Shield

(connected

to DGN D)

0

VDC

Warnings and precaut ions

991-025 1 Rev. 1.3 June-2 016 xiv

The u ser should b e made aware of that, if the NucleoCounter® NC-250™ and the

external power supply is used in a manner not sp ecified by the manufacturer, t he
protection provided by the NucleoCounter® NC-250™ and the external power supply may
be impaired.

Electromagnetic interference

NOTE: This equipment has been tested and found to comp ly with the limits fo r a Cla ss B
digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide
reasonable protection against harmfu l interference in a residential installation. This
equipment gen erates, uses and can radiate radio frequency energy and, if not installed and
used in accordance with the instruct ions, may cause harmful interferenc e to ra dio
communication s. However, there is no guarantee that inter ference will not occur in a
particular in stallation. If t his equipment does cause harmful interference to radio or
televi sion reception, which can be determined by turning the equipment off and on, the
user i s encouraged to try to correct the interference by one or more of the foll owing
measur es:

 Reorient or r elocate the re cei ving antenna.

 Increase the separation between the equipment and receiver.

 Connect the equipment to an outlet on a circuit different from that to which the

receiver is connected.

 Consult the dealer or an experienced radio/ TV technician for help.

Caution! Changes or modif ications not expressly approved by the party responsible f or
compliance could void the use r's authority to operate the equipment.

Slides, Reagents, Solutions and Dispensers

With r espect to us e and handling of slides, reagent s, solutions and dispensers, please refer
to appropriat e package insert s for these it ems.

Caution! When using a bottle-top dispenser: To protect against accidental splashes ,
protective clothing, eye protection and glo ves must be worn when using potentially
hazardous liquids.

General

Any biological specimen should be handled , as if it is capable of transmitting infectious
disease and disposed of with proper precaut ions according with federal, state and local
regulations.

Avoid specimen contact with skin or mucous membranes.