ChemoMetec NC-3000 User Manual
NucleoCounter® NC-3000
User's Guide
P/N 991-3001
Revision 1.3
™
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NucleoCounter
NC-3000™
Advanced Cell Analyzer
P/ N 9 91 -3 00 1 (E nglis h)
Re vi s io n 1. 3
Ju ne, 2 01 3
ChemoMetec A/S
Gydevang 43, DK-3450 Allerod, Denmark
Telephone: (+45) 48 13 10 20, Fax: (+45) 48 13 10 21
Internet: www.chemometec.com,
E-mail: Sales@chemometec.com
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Caution!
This equipment must be operated as described in this User’s Guide and documents referred to herein.
Please read the entire guide and referred documents before attempting to use this unit. Please pay
attention to that gloves or protective clothing are not worn on the illustrations/pictures shown in this
User’s Guide. However, Chem oMetec A/S does recommend that the user wear suitable prot ective
clothing etc.
Contacting support
Technical information including product literature, answers to questions regarding the operation of the
NucleoCounter NC -3000™ not covered in this document and referred documents is available through
the following:
For e-mail support, send questions to NucleoCounter NC-3000™ Technical Support on the
address Support@chemometec.com
Check out the FAQ section under support at www.chemometec.com
To speak with a Technical Support Specialist, call (+45) 48 13 10 20.
Please note the NucleoCounter NC-3000™ serial number and have it available when contacting
ChemoMetec A/S for support. The NucleoCounter NC-3000™ serial number is found on the label
affixed to the rear of the instrument. The version number of the NucleoView NC-3000™ software shall
also be noted, this can be found on the Help – About menu item in the NucleoView NC-3000™
software.
Sales and ordering information
For sales assistance with NucleoCounter NC-3000™ or the NucleoView NC-3000™ software, to
place an order for a NucleoCounter NC-3000™ or consumables, call (+45) 48 13 10 20, fax (+45) 48
13 10 21, or send e-mail to Sales@chemometec.com
Disclaimer Notices
The material in this document and referred documents is for information only and is subject to change
without notice. While reasonable efforts have been made in preparation of these documents to assure
their accuracy, ChemoMetec A/S assumes no liability resulting from errors or omissions in these
documents, or from the use of the information contained herein.
ChemoMetec A/S reserves the right to make changes in the product design without reservation and
without notification to its users.
Copyright Notices
Copyright © ChemoMetec A/S 2010. All rights reserved. No part of this publication and referred
documents may be reproduced, stored in a retrieval system, or transmitted in any form or by any
means, electronic, mechanical, photocopying, recording or otherwise, without the prior written consent
of ChemoMetec A/S, Gydevang 43, DK-3450 Allerod, Denmark.
ChemoMetec and NucleoCounter® are registered trademarks owned by ChemoMetec A/S.
NucleoCounter, NucleoCassettes, and NucleoView are trademarks of ChemoMetec A/S.
All other trademarks are the property of their respective owners.
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Declaration of Conformity
Directives
Electromagnetic Compatibility (2004/108/EC). EMC
Low Voltage Directive (2006/95/EC) LVD
Machinery Directive (2006/42/EC) MD
Standards
EMC:
EN 61326-1:2006 Electrical equipment for laboratory use
EN 61000-3-2: 2006 Limits for harmonic current emissions
EN 61000-3-3: 1995 +A1 +A2:2005 Limitation of voltage changes,
voltage fluctuations and flicker
Standards
LVD:
EN 61010-1:2001 Safety requirements for electrical equipment for
measurement, control, and laboratory use
Standards
MD:
EN 12100-1: 2003 Safety of machinery; Basic concepts, general
principles for design — Part 1
EN 12100-2: 2003 Safety of machinery; Basic concepts, general
principles for design — Part 2:
EN ISO 14121-1:2007 Safety of machinery; risk assessment
Declaration of Conformity
Name of product: NucleoCounter
Type: Advanced Cell Analyzer
Other identifying data: Part no. 900-3001
We declare under our sole responsibility that the products, to which this declaration
relates, are in conformity with the Council Directives on the approximation of the
laws of the EEC Member States relating to the following:
NC-3000™
This device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions: (1) this device may not cause harmful interference, and (2)
this device must accept any interference received, including interference that may
cause undesired operation.
Date: 2010-May-1st Signed:
Name: Sarah R. Rasmussen
Position: QA/QC Managers
Name and address of manufacturer:
ChemoMetec A/S
Gydevang 43
DK-3450 Allerod
DENMARK
www.chemometec.com
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Declaration of Conformity
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WEEE directive information – Europe only
Correct Disposal of This Product
(Waste Electrical & Electronic Equipment) - Europe only
This marking shown on the product or its literature, indicates that it
should not be disposed together with other household wastes at the
end of its working life. To prevent possible harm to the environment or
human health from uncontrolled waste disposal, please separate this
from other types of wastes and recycle it responsibly to promote the
sustainable reuse of material resources.
Business users should contact their supplier and check the terms and
conditions of the purchase contract. This product should not be mixed
with other commercial wastes for disposal.
This information is listed in “Appendix B: WEEE directive information in
more EU languages”.
WEEE directive information – Europe only
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WEEE directive information – Europe only
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Introduction and intended use
Introduction and intended use
The NucleoCounter
®
NC-3000™ is a compact cell-analyzer intended to be used for
advanced cell analysis within the life-science and pharmaceutical markets. The
NucleoCounter
diagnostic use. The NucleoCounter
®
NC-3000™ system is intended for research use only, not for
®
NC-3000™ uses different types of disposable
Cassettes from ChemoMetec A/S and special designed slides with 2 or 8 chambers.
This will cover an analysis volume from 0.8µl to 16µl.
The NucleoCounter
®
NC-3000™ is a highly advanced cell analyzer based on
fluorescence microscopy using CCD camera technology with low magnification and
advanced image analysis. Numerous properties of the individual cells can be
analyzed with the 8 integrated light sources (7 of them illuminating from 365 nm to
625 nm and a white light source) and with 9 emission filters. Spatial position is
maintained from filter to filter enabling direct comparison of the different parameters
detected or quantified on each individual cell. The low magnification (x2) guarantees
a high analysis volume and thus ensures a high statistical performance.
Specially designed sample illumination eliminates the risk of bleaching of adjacent
sample material and therefore several images can be exposed in the same sample
window. Since no tubes, valves, hoses etc. are present the instrument is virtually
service and maintenance free.
The NucleoCounter
®
NC-3000™ part number at ChemoMetec A/S is 900-3001.
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Introduction and intended use
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Warnings and precautions
Pin no.
Name
Maximum
Voltage level1
1
+5V
+5
VDC 2 D-
+3.5
VDC 3 D+
+3.5
VDC 4 DGND
0
VDC
Metal
enclosure
Shield
(connected
to DGND)
0
VDC
Warnings and precautions
Whenever the symbol appears on the NucleoCounter
®
NC-3000™ instrument, it
indicates that the manual must be consulted for precautions and warnings.
Power and cables
Use the shielded USB cable supplied with the NucleoCounter
that appropriate EMI classification is maintained for the intended environment.
The USB interface connector of the NucleoCounter
®
connected to SELV circuits. External computing devices connected to the USB
interface connector of the NucleoCounter
®
NC-3000™ has to comply with the
standards, UL 1950 and IEC/EN 60950.
Table 1. Description of the NucleoCounter
®
USB interface connector
®
NC-3000™ to ensure
NC-3000™ must only be
NucleoCounter
®
NC-3000™ is powered by an external 24VDC power supply. For
safe use, please follow the instructions for connecting the power supply.
The NucleoCounter
®
NC-3000™ shall only be used with an external power
supply purchased at ChemoMetec A/S.
The detachable DC power supply cord set and appliance inlet of the external
power supply are considered as the disconnecting device.
The mains supply cord and plug of the external power supply shall comply with
any national regulations.
1
In normal operation mode (refers to Pin no 4)
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Warnings and precautions
The user should be made aware of that, if the NucleoCounter
®
NC-3000™ and
the external power supply is used in a manner not specified by the manufacturer,
the protection provided by the NucleoCounter
®
NC-3000™ and the external power
supply may be impaired.
Electromagnetic interference
NOTE: This equipment has been tested and found to comply with the limits for a
Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are
designed to provide reasonable protection against harmful interference in a
residential installation. This equipment generates, uses and can radiate radio
frequency energy and, if not installed and used in accordance with the instructions,
may cause harmful interference to radio communications. However, there is no
guarantee that interference will not occur in a particular installation. If this
equipment does cause harmful interference to radio or television reception, which
can be determined by turning the equipment off and on, the user is encouraged to
try to correct the interference by one or more of the following measures:
Reorient or relocate the receiving antenna.
Increase the separation between the equipment and receiver.
Connect the equipment to an outlet on a circuit different from that to which
the receiver is connected.
Consult the dealer or an experienced radio/TV technician for help.
Caution! Changes or modifications not expressly approved by the party responsible
for compliance could void the user's authority to operate the equipment.
Cassettes, Slides, Reagents, Solutions and Dispensers
With respect to use and handling of Cassettes, slides, reagents, solutions and
dispensers, please refer to appropriate package inserts for these items.
Caution! When using a bottle-top dispenser: To protect against accidental splashes,
protective clothing, eye protection and gloves must be worn when using potentially
hazardous liquids.
General
Any biological specimen should be handled, as if it is capable of transmitting
infectious disease and disposed of with proper precautions according with federal,
state and local regulations.
Avoid specimen contact with skin or mucous membranes.
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Warnings and precautions
Never pipette by mouth.
Avoid cross contamination of the samples when preparing the samples. This can
compromise the quality of the results.
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Warnings and precautions
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