ChemoMetec NC-250 User Manual
Quick Guide
Viability & Cell Count, Cell Health & Cell Cycle
in one instrument!
®
NucleoCounter
NC-250
™
How to get started
Dear NucleoCounter Customer!
Thank you for purchasing the NucleoCounter® NC-250™ offering not only easy Viability and Cell
Count Assays, but also fast and easy assaying of cell cycle profiles with top class precision and
the fastest apoptosis assay!
The NucleoCounter® NC-250™ Concept
8 viability and cell counts in less than 3
minutes
- Total cell count
- Viability
- Cell diameter
- % of cells in clumps
Up to 8 advanced analysis in one run
- Fast & Easy - High precision cell cycle analysis
- Cell health determinationvia the fastest
apoptosis assay
Fast and easy operation
Excellent reproducibility
No calibration
Maintenance and service free
Flexible software package
21 CFR Part 11 ready
NucleoView™ Software
Included on a USB stick
for an unlimited no. of instal-
lations.
A8 slides for fast analysis and A2 slides for high
NC-Slides A8™ and A2
precision.
How to get started - 8 easy steps to install the NC-250
™
Solution 18
For Viability and Cell Count
Assays.
™
Unpack the NC-250 instrument and plug it in the main outlet. Do NOT connect the
USB cable to the PC.
Make sure that there are full administrator rights on the PC during the installation of
the NucleoView™ NC-250 software.
Insert the USB stick in the PC and open the “Install_Guide” file for detailed installation instructions.
Open the “Install NucleoView NC-250 X.X.X.X.exe” file (the X’s indicate the version
number e.g. 1.0.22.0). Do NOT open the .bin file.
Follow the instructions on the screen. After the software installation it may be required to restart the PC.
Open the NucleoView™ NC-250 software by double clicking on the NC-250 icon on
the desktop to start the installation of the instrument.
Connect the PC to the NC-250 instrument with the USB cable when the software tells
you to do so.
When the LED indicator light on the instrument turns green it is ready to use.
Please read manual for detailed instructions
1
How to Perform Viability and
Cell Count Analysis
Fast and Easy Cell Count and Viability Assays!
The NucleoCounter® NC-250™ from ChemoMetec is a significant step forward for
automated cell counting.
Select the desired protocol from the menu:
For example ‘Viability and Cell Count Assay’
Choose the media type (A8 or A2 slide)
Select the chambers to perform the assay on
Optional: Enter Sample ID and Operator Name
Mix the cell sample with the assay defined solution(s), load the
slide and Press ‘Run’
2
3
4
1
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