C.B.S. Scientific DTSK-2401-220 User Manual

Download

INSTRUCTION MANUAL

Cipher GenetiC AnAlysis system

DTSK-2001-110 DTSK-2001-220 DTSK-2401-110 DTSK-2401-220

One System that Detects Mutations and Polymorphisms 4 different ways:

1) DGGE - Denaturing Gradient Gel Electrophoresis

2) TTGE - Temporal Temperature Gradient Electrophoresis

3) SSCP - Single Strand Conformational Polymorphisms

4) CDGE - Constant Denaturant Gel Electrophoresis

Cipher Genetic Analysis System

www.cbsscientic.com

DTSK Instruction Manual, version 8/30/2011

TABLE OF CONTENTS

Important User Information 3-4

Section 1 General Information

1.1 Introduction 5

 1.2 Specications       6  1.3 Safety      6

Section 2 Description of parts

2.1 Unpacking 7

2.2 Components/Assembly 7

Section 3 Instructions for Use

3.1 Unit Set-up and Unpacking 8-9

3.2 Heater/Stirrer General Set-Up 10-24

3.3 Heater/Stirrer Programming 25-34

3.3.1 Setting Operational Parameters and Functions 25

  3.3.2Entering/ModifyingaTemperatureProgram  26-30

3.3.3 Running a Temperature Program 31-32

3.3.4 Setting Preferences 32

3.3.5 Heater/Stirrer Error Messages 33-34

3.4 Running the Heater/Stirrer 35

3.5 Preparation/Cleaning of Glass Plates 35

 3.6 GelCastingTechniques      36-37

3.7 Vertical Gradient Formation 38-42

3.8 Preparation of the Cassettes 43

3.9 Buffer Cycling Connections 44

3.10 Running the Gels 45

 3.11 RemovingtheGels       46

3.12 Perpendicular Gel Casting 47

3.13 Perpendicular Gradient Formation 48-49

3.14 Buffer Siphon Pump Instructions for use 50

3.15 Maximum Well/Comb Volumes 50

Section 4 Running Conditions 51

4.1 Recommended Power 51

4.2 Recommended Buffers 51

4.3 Recommended Running Conditions 52

4.4 SSCP Running Conditions 52

4.5 References 53

Section5 MaintenanceofEquipment

5.1 Care and Handling 54

5.2 Maintenance & Calibration 55

Section6 Heater/StirrerTroubleshooting     56-57

Section 7 Accessories 58

IMPORTANT USER INFORMATION

This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety.

The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/ or warranty requirements.

The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous electrical exposure.

Failure to adhere to the instructions could result in personal and/or laboratory hazards, as well as invalidate any warranty. Always turn off the DC power source prior to disconnecting power cords from the product. Disconnect

power cords from the power source rst, and then from the product. For maximum safety, always operate this system in an isolated, low trafc area, not accessible to unauthorized personnel. Never operate damaged or

leaking equipment.

WARRANTY AND LIABILITY

This product was produced utilizing the highest practical standards of materials, workmanship, and design. C.B.S. Scientic warrants that the product has been tested and will meet or exceed published specications. This warranty is valid only if

the product has been operated and maintained according to the instructions provided.

C.B.S. Scientic warrants this product to be free from defects in materials and workmanship under normal service for one year from date of shipment. If the product proves defective during this period, C.B.S. Scientic will repair or replace it at our

option, free of charge, if returned to us postage prepaid. This warranty does not cover: damage in transit, damage caused by carelessness, misuse or neglect, normal wear through frequent use, damage caused by solvent corrosion, damage caused by improper handling or user alteration, nor unsatisfactory performance as a result of conditions beyond our

control. C.B.S. Scientic shall in no event be liable for incidental nor consequential damages, including without limitation, lost prots, loss of income, loss of business opportunities, loss of use and other related damages, however caused, nor any

damage arising from the incorrect use of the product.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURS

Le présent manuel d’utilisation explique la manière de se servir efcacement du produit en conditions

de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions.

Le triangle avec point d’exclamation est un symbole destiné à avertir l’utilisateur du produit

de l’importance de certaines exigences relatives au fonctionnement, à l’entretien et/ou à la garantie.

Le triangle avec èche en zigzag est un symbole destiné à avertir l’utilisateur du produit de la

possibilité d’exposition à des décharges avec danger de secousses électriques.

Tout manquement à l’observation des consignes et des instructions peut exposer les personnes et les biens à des dommages corporels et/ou matériels et peut annuler toute garantie. Il faut toujours interrompre l’alimentation de courant continu avant de déconnecter les cordons d’alimentation du produit. Déconnecter d’abord les cordons d’alimentation branchés sur la source de tension (alimentation de secteur) puis ceux branchés sur le produit. Pour une sécurité maximum, il faut toujours faire fonctionner ce système dans un lieu isolé, peu fréquenté, où le personnel non autorisé n’a pas accès. Ne jamais faire fonctionner un matériel endommagé ou affecté par des fuites.

GARANTIE ET RESPONSABILITÉ

Le produit a été fabriqué conformément aux normes applicables les plus exigentes en matière de

matériaux, de main d’oeuvre, de conception et d’ingéniérie. C.B.S. Scientic garantit que le produit a subi des essais et que ses performances rempliront les conditions des spécications publiées ou luer seront

même supérieures. La présente garantie n’est valide que si le produit a fonctionné et a été entretenu conformément aux consignes et instructions fournies.

C.B.S. Scientic garantit que le produit sera dépourvu de vices de matériaux et de main d’oeuvre, en

conditions de service normales, pendant un an à compter de la date d’expédition. Au cas où le produit

s’avérerait défectueux pendant cette période de garantie, C.B.S. Scientic réparera ou remplacera le

produit, à sa discrétion et gratuitement, si le produit lui est retourné port payé d’avance. La garantie ne couvre pas les dommages de transport; les dommages causés par l’imprudence, le manque de soins, l’abus ou la négligence; l’usure normale résultant d’une utilisation fréquente; les dommages causés par la corrosion des solvants; et les dommages causés par la manipulation inadéquate ou des changements apportés par l’utilisateur. La garantie ne couvre pas non plus les performances non

satisfaisantes résultant de conditions hors du contrôle de C.B.S. Scientic. C.B.S. Scientic ne pourra

en aucun cas être tenue responsable de dommages indirects, y compris, de manière non limitative, la

perte de bénéces, le manque à gagner, la perte d’occasions d’affaires, l’impossibilité d’usage ou tous

autres dommages associés, quelle qu’en soit la cause, ni de dommages résultant de l’usage incorrect du produit.

DEUTSCH WICHTIGE INFORMATION FÜR DEN BENUTZER

Diese Bedienungsanleitung beschreibt wie man dieses Produkt sicher und wirksam benutzt. Bitte lesen und befolgen Sie alle Anweisungen in dieser Anleitung.

ESPAÑOL INFORMACIÓN IMPORTANTE PARA EL USUARIO

El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.

El símbolo del triángulo con exclamación llama la atención del usuario a requisitos importantes para el uso y mantenimiento del producto, así como para la validez de la garantía.

El símbolo del triángulo con rayo llama la atención del usuario a la posibilidad de riesgos eléctricos.

El incumplimiento de las instrucciones aquí señaladas podría dar lugar a riesgos a la persona, al laboratorio o a ambos y podría anular toda garantía. Siempre apague la fuente de corriente continua antes de desenchufar los cables eléctricos del producto. Primero desconecte los cables de la fuente de energía y después del producto. Para mayor seguridad, siempre use este sistema en un área aislada, de poco movimiento de personas e inaccesible a personal no autorizado. Jamás use equipo que presenta algún daño o fuga.

GARANTÍA Y RESPONSABILIDAD

Este producto fue fabricado de acuerdo con las normas más estrictas que sean factibles en cuanto a

materiales, mano de obra y diseño. C.B.S Scientic garantiza que se sometió el producto a pruebas y que cumplirá o excederá las especicaciones publicadas. Esta garantía será válida únicamente si se

usa y se da servicio de mantenimiento al producto de acuerdo con las instrucciones señaladas.

C.B.S. Scientic garantiza que este producto se encontrará libre de defectos de materiales y mano de

obra por un período de servicio normal de un año a partir de la fecha de embarque. Si el producto resulta

defectuoso durante este período, C.B.S. Scientic lo reparará o lo repondrá, a criterio de C.B.S., libre

de cargos, si se devuelve el producto a C.B.S. porte pagado. Esta garantía no cubre daños sufridos en tránsito, daños provocados por descuido, mal uso o negligencia, desgaste normal como consecuencia del uso excesivo, daños atribuibles a corrosión provocada por solventes, daños causados por el uso indebido o alteraciones realizadas por el usuario ni rendimiento insatisfactorio atribuible a circunstancias

fuera del control de C.B.S. Scientic. C.B.S. Scientic en ningún caso asumirá responsabilidad por

daños incidentales o subsecuentes, incluyendo, en forma no limitativa, la pérdida de utilidades, de

ingresos, de oportunidades comerciales o del uso del producto y otros daños anes, fuere cual fuere su

origen, ni por daños derivados del uso incorrecto del producto.

ITALIANO INFORMAZIONI IMPORTANTI PER L’UTENTE

Questo manuale spiega come utilizzare questo prodotto in maniera sicura ed efciente. Si pregai di

leggere e seguire con cautela le istruzioni di ogni parte di questo manuale.

Das Dreieck mit Ausrufezeichen weist den Benutzer des Produktes darauf hin, daß wichtige Bedienungs-, Wartungs- und/oder Garantievorschriften zu beachten sind.

Das Dreieck mit Zickzackblitz warnt den Benutzer des Produktes vor möglichen Gefahren durch elektrische Spannungen.

Nichtbeachtung dieser Anweisungen kann zu persönlichen und/oder labortechnischen Schäden führen und gleichzeitig alle Garantien als nichtig erklären. Die DC Stromzufuhr muß immer, vor dem Entfernen der Stromkabel vom Produkt, abgeschaltet werden. Die Stromzufuhrkabel müssen zuerst von der Steckdose und erst dann vom Produkt entfernt werden. Um höchste Sicherheit zu gewährleisten sollte dieses System in einem abgesonderten und besonders ruhigen Bereich eingesetzt werden und vor Unbefugten sicher sein.

GARANTIE UND HAFTUNG

Dieses Produkt wurde unter Anwendung von Produkten mit höchster Qualität und aus Materialien mit

bester Verarbeitung und modernstem Design hergestellt. C.B.S Scientic garantiert, daß das Produkt getestet wurde und alle publizierten Spezikationen übertrifft. Diese Garantie ist jedoch nur gültig, wenn

das Produkt nach der beigefügten Bedienungsanleitung bedient und gewartet wurde.

C.B.S. Scientic garantiert, daß dieses Produkt bei normaler Bedienung aus fehlerfreiem Material

besteht und fehlerfrei in der Ausführung ist. Diese Garantie gilt für ein Jahr ab Lieferdatum. Sollte

das Produkt in diesem Zeiraum fehlerhaft werden, bietet C.B.S. Scientic eine kostenlose Reparatur bzw. Kostenlosen Ersatz, einschließlich freiem Rückporto. Diese Garantie schließt folgendes aus:

Transportschaden, Schaden durch Nachlässigkeit, Mißbrauch oder Vernachlässigung, normale Abnützung durch regelmäßigen Gebrauch, Schaden durch Säureangriff, Schaden durch falsche Handhabung, Veränderung des Produktes durch den Benutzer, oder unzureichende Leistungen die

sich nicht im Verantwortungsbereich von C.B.S. Scientic benden. C.B.S. Scientic kommt unter

keinen Umständen für folgende Schäden auf: Sachschadensverlust, Einkommensverlust, Verlust von Geschäftsmöglichkeiten, Verlust der Anwendung und andere damit verbundene Schäden die auf irgend eine Art und Weise entstanden sind, oder Schäden die aus falscher Anwendung des Produktes entstanden sind.

Il triangolo contenete il simbolo di un punto esclamativo avverte l’utente di importanti requisiti relativi al funzionamento, manutenzione e/o garanzia del prodotto.

Il triangolo contenete il simbolo di un lampo avverte l’utente del prodotto della possibilità di pericoli dovuti a corrente elettrica.

La mancata osservanza delle istruzioni può essere causa di pericolo alla propria persona ed al laboratorio, oltre a poter annullare la garanzia. Prima di distaccare il cordone d’alimentazione dal prodotto, spegnere sempre la sorgente di corrente continua. Distaccare i cordoni d’alimentazione prima dal lato della sorgente di tensione e poi dal lato del prodotto. Per maggior sicurezza, mettere sempre

in funzione il prodotto in un’area isolata con poco trafco che non sia accessibile al personale non

autorizzato. Non mettere mai in funzione un’apparecchiatura che sia danneggiata o abbia perdite.

GARANZIA E RESPONSABILITÀ

Questo prodotto è stato fabbricato seguendo gli standard più elevati per i materiali, la manodopera e la progettazione. La C.B.S. Scientic garantisce il prodotto è stato sottoposto a prova e raggiunge o supera i valori pubblicati per i dati tecnici. Questa garanzia è valida solo se il prodotto è messo in esercizio e soggetto a manutenzione secondo le istruzioni fornite.

La C.B.S. Scientic garantisce che questo prodotto è libero di difetti di materiali e manodopera, in normali

condizioni d’esercizio, per la durata di un anno dalla data di spedizione. Se, in questo periodo, il prodotto

si dimostrerà difettoso, la C.B.S. Scientic, a suo giudizio, lo riparerà o sostituirà. Questa garanzia non

copre danni in transito, danni causati da negligenza, uso improprio, trascuratezza, normale consumo derivante da uso frequente, o danni causati da solventi corrosivi, danni causati da maltrattamento o da

modiche apportate dall’utente e non copre prestazioni insoddisfacenti che siano il risultato di condizioni al di fuori del controllo del fabbricante. La C.B.S. Scientic non sarà in ogni caso responsabile per danni incidentali o consequenziali, incluso, senza limitazioni, perdite di protto, perdita di entrate, perdita di

opportunità d’affari e altri danni relativi, comunque causati, e per danni risultati da uso incorretto del prodotto.

SECTION 1 General Information

1.1 Introduction

DESCRIPTION

The Cipher GenetiC AnAlysis systems (DTSK-2001 and DTSK-2401) allow the researcher to choose which technique is most applicable to their investigation. Both DGGE and TTGE rely on establishing a gradient of either solvent (Urea/Formamide) or temperature in which the target fragments will undergo conformational transition (melt). This

sequence dependent information will determine the theoretical melting behavior of the target fragment after PCR amplication. If the sequence is known, then primer/probe design can be made using certain software design

programs (1). If not, the melting range can be revealed by running perpendicular DGGE/TTGE gels. The third mutation detection technique, SSCP, differentiates between normal and mutant duplex DNA by denaturing the DNA to form single stranded molecules of equal length. These molecules can re-anneal onto themselves and based on the varying degree of intrastrand base pairing, form different three-dimensional structures. These structures, differ in electrophoretic mobility and can be separated on a non-denaturing polyacrylamide gel.

C.B.S. SCIENTIFIC has designed two different Cipher GenetiC AnAlysis systems which are reliable and easy-to-use. The DTSK-2001 is a 2 gel system and includes two single gel cassettes. The DTSK-2401 is a four gel system which includes two dual gel cassettes. The systems feature: programmable heater/stirrer which can be programmed for

DGGE, TTGE and SSCP applications, a large diameter cooling coil for use with an external chiller, a simplied

method for casting perpendicular and vertical gels using Gel Wrap®, single or dual gel cassettes, an internal impellor pump for buffer cycling, polypropylene spring clamps, and a safety cover with an electrical interlock which helps maintain temperature, reduce evaporation, and protect against shock hazard.

DGGE & CDGE

Denaturing Gradient Gel Electrophoresis (DGGE) is a powerful genetic analysis technique that can be used for

detecting single base changes and polymorphisms in genomic (2,3), cloned, and PCR amplied DNA (3,4). Two

of the most valuable uses for DGGE in human, animal or microbial genetics are in directly detecting single base changes that cause disease and in detecting polymorphisms with DNA probes for genetic-linkage analysis. In DGGE, conformational transitions of multiple nucleic acid complexes are induced by an increasing concentration of solvent (Urea/Formamide) at a constant temperature. Clinical applications of DGGE include a rapid and effective method for screening samples for genetic mutations and variants. Also, DNA fragment melting points can be determined using perpendicular DGGE (2). In contrast to DGGE, CDGE (Constant Denaturant Gel Electrophoresis) uses a single solvent percentage to induce partial melting of DNA fragments as they enter the gel. The disadvantage of CDGE is that only a single melting domain can be interrogated.

TTGE

Destabilization of nucleic acid complexes can also be studied using acrylamide gels which contain a uniform solvent concentration (Urea/Formamide), but with an increasing temperature gradient (6). Since the temperature of the entire gel is uniformly raised over a period of time, this technique has been termed ‘TTGE’, or Temporal Temperature Gradient Electrophoresis (7). This technique incorporates many improvements over DGGE/CDGE especially when studying multiple melting domains (8).

SSCP

Single Strand Conformational Polymorphism (SSCP) reveals differences in electrophoretic mobility between normal and mutant single strands of DNA (9). In SSCP, normal and mutant duplex DNA are denatured to form single stranded molecules of equal length. These molecules can re-anneal onto themselves and based on the varying degree of intrastrand base pairing, form different three-dimensional structures. These structures, differ in electrophoretic mobility and can be separated on a chilled non-denaturing polyacrylamide gel. The electrophoretic mobility of these “conformers” change depending on temperature and buffer ionic strength. For accurate characterization of mutations within these re-folded single strands, it is essential that the buffer temperature be tightly controlled within each electrophoresis procedure, usually between 4º-25ºC. If the gel temperature is not precisely controlled, the resolution will suffer because of the loss of intrastrand base pairing and change in the overall shape of the 3-dimensional conformer.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

1.2Specications

Constructions

DTSK Tank Sodalime glass, Kydex DTSK Lid Sodalime glass, Kydex

Electrodes Platinum wire .012” diameter Power cords FR Polypropylene/Silicone, rated 7500V, 200mA, 65° C DTSK Cassette Acrylic

Cooling Coil brass, copper, PVC Heater/Stirrer Buffer Cycler Stainless steel Combs Teon/ Acrylic Spacers Proprietary Glass Plates Sodalime or Borosilicate

Gradient Maker Acrylic, teon, stainless steel

DTSK-2401

Gel Size Distance between electrodes Voltage limit

17.7cm x 22cm

20cm

250

1.3 Safety

Power to the DTSK Systems is to be supplied by an external DC voltage power supply that must be ground isolated

so that the DC voltage output oats with respect to ground. For any power supply used, the maximum specied

operating parameters for the units are:

Maximum Limits

250 VDC voltage 60 watts power 1600 mA current 70°C ambient temperature

Current to the unit, provided from the external power supply, must enter the unit through the lid assembly, providing a safety interlock to the user. Current to the unit is broken when the lid is opened. Do not attempt to use the unit

without the safety lid. Always turn the power supply off before removing the lid, or when working with the

unitinanyway.Followsafetyprecautionsspeciedbythepowersupplymanufacturer.

SECTION 2

Heater Stirrer

Buffer Siphon Pum p Assemb ly

TTG E Tank

Dual Cassette (2)

Com b (4 )

Spacers (4 pairs)

Glass Plate Assembly(4)

Clamps

Mini-Pump (Optiona l)

GM-40

Gelwrap

(4)

Description of Parts

2.1 Unpacking

Please verify that your Cipher GenetiC AnAlysis system comes complete with the following components:

• Lower Reservoir/Glass tank/safety interlock/ with 2 black leads inside lid

• Heater/Stirrer/By-pass pump/Cooling Coil

• Two Dual Gel cassettes

• 1 Gradient maker, 20mls per side, Cat. # GM-40

• 8 each combs, 4 sets glass plates, 8 pair spacers and 8 each Gel Wrap gaskets

• White spring clamps, 12 each GPC-0002-177 and 16 each GPC-0001-177 (Number of clamps received with

system will vary depending on format of DGGE)

• Buffer siphon pump with tubing, Cat. # BSP-100

• EPS-300-II Power Supply

• Peristaltic Mini-Pump for gel casting

2.2 Components/Assembly

www.cbsscientic.com

Cooling coil is

situated inside tank

below heater/stirrer

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

SECTION 3 Instructions for Use

3.1 Unit Set-up and Unpacking Instructions

1. Unpack lower reservoir and place on level surface in an approved location.

2. WARNING:DoNOTturnonHeater/Stirreruntiltankhasbeenlledwithbuffer. The MINIMUM buffer

volume for heating is 23 liters. Turning Heater/Stirrer on with less than 23 liters of buffer will damage the heating coil, and void warranty. If running two dual cassettes, the buffer volume is 24 liters.

3. Connect cooling coil to chiller using tubing adapters and 1/4th I.D. tubing (tubing is not supplied).

Fig. 3-1

cooling coil inlet

and outlet

heater/stirrer

3.1 Unit Set-up and Unpacking Instructions-continued

4. Position an appropriate power supply on a shelf above the tank.

5. Fill the tank with running buffer. To obtain the correct level of buffer, place the cassettes in the tank and ll

the tank until the level of buffer reaches the underside of the upper reservoir. The upper reservoir holds only 50mls.

6. Attach the power supply to the safety interlock. (Fig 3-2-A)

7. Attach the safety interlock to a wall (mains) outlet. (Fig 3-2-B)

8. Plug the Heater/Stirrer into a wall (mains) outlet. Important: Do not plug the heater/stirrer into anything other

than a wall outlet.

9. Do NOT attach power leads (from power supply to safety interlock, Fig 3-2-C) until gels are loaded and

installed in the tank.

Power Supply/Safety Interlock Connections

Fig. 3-2

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.2 Heater/Stirrer Set-Up and Programming

3.2a General Information

Model Type Temperature

Heat Only

Immersion Circulator

Circulator Pump

Pump speed is selected via the Main Menu. The MINIMUM setting is used for DGGE applications.

MAXIMUM is recommended where temperature varies frequently and there is a need for fast recovery or when pumping to multiple external units.

Speed Maximum Pump Outlet Ratings Maximum Pump Outlet Ratings Selection Line Frequency = 60Hz Line Frequency = 50Hz

MAXIMUM 30 LPM / 5.0 PSI 22 LPM / 3.4 PSI

HIGH 24 LPM / 2.5 PSI 20 LPM / 1.7 PSI

MEDIUM 20 LPM / 1.7 PSI 17.5 LPM / 1.2 PSI

LOW 18 LPM / 1.2 PSI 15.8 LPM / 0.8 PSI

MINIMUM 15.7 LPM / 0.8 PSI 15 LPM / 0.7 PSI

Range

Ambient +5°C to

200°C*

Reservoir

Capacity

N/A 11A 9.7A

Amps @

120V,60Hz

Amps @

240V, 50Hz

The data in the table above are based on the following criteria:

a. Maximum pump outlet ow rate is measured in liters per minute (LPM) with no restriction on the pump outlet.

b. Maximum pump outlet pressure is measured in pounds per square inch (PSI) at no ow.

c. Water was used as the circulation uid. Water has a viscosity of one centistoke. High viscosity or low-density uids will change

these gures.

Filling the Reservoir

A liquid level that fully covers the heater coil, pump, over-temperature sensor, and at least one inch (25mm) of the temperature sensor must be maintained. For heating applications, the DTSK tank must have at least 23 liters of buffer. When running the system with two dual cassettes, buffer volume should be 24 liters.

Temperature Sensor

minimum ll level

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2b Front and Rear Panels

Front View Rear View

8 9

1. Select / Set Knob 10. Identication Label

2. LCD Display 11. Reservoir Purge

3. Safety Set Indicator Knob 12. Pump Inlet (Do NOT open)

4. Safety Set Reset Button 13. Pump Outlet (Do NOT open)

5. Timer Button 14. AC Input

6. Escape Button 15. External Probe Connection

7. Power On/Off Button 16. RS232 Interface

8. Heating Light 17. Circuit Breaker / AC Power Switch

9. Cooling Light1

Active on Refrigerating/Heating Circulating Baths only

Functional on Programmable Controller only.

www.cbsscientic.com

Heater/Pump Assembly

1. Temperature Sensor

2. Pump Shaft and Impeller

3. Over-Temperature Sensor

4. Heater Coils

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2cSpecications

Temperature Stability

Controller / RS232 Yes

External Temperature Probe Functional on Programmable models / optional external

Readout Accuracy Graphic LCD, °C or °F, ±0.25°C

Heater 1100W – 115V, 2200W – 240V

Pressure Flow Rate 15.7 to 30 LPM (60Hz); 15 to 22 LPM (50Hz)

Suction Flow Rate 11 to 22 (60Hz); 10 to 15 LPM (50Hz)

Over-Temperature Protection Yes, user-adjustable

Low-Liquid Protection Yes

Pump Speeds Five-speed adjustable

1. Temperature stability may vary depending on bath volume, surface area, insulation, and type of uid.

NOTE: Performance specications determined at ambient temperature of 20°C (68°F).

±0.01°C

probe required

Not functional on Digital models

Environmental Conditions:

• Indoor Use Only • Over Voltage: Category ll

• Maximum Altitude: 2000 meters • Operating Ambient: 5° to 30°C

• Relative Humidity: 80% for temperatures to 30°C • Pollution Degree: 2

• Class 1: Residential, Commercial, Light Industrial • Class 2: Heavy Industrial

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2d Circulator Location

Locate the Circulator on a level surface, free from drafts and out of direct sunlight. Do not place it where corrosive fumes, excessive moisture, high room temperatures, or excessive dust are present.

Refrigerating/Heating Circulators must be a minimum of four inches (102mm) away from walls or vertical surfaces so air ow around the unit is not restricted.

To help prevent voltage drops, position the Circulator as close as possible to the power distribution panel and a properly grounded outlet. The use of an extension cord is not recommended.

Warning: These units are equipped with over-temperature protection (Safety Set). A low liquid level or failure to set the Safety Set and properly immerse the heater may result in heater burnout and triac failure. While operating, do not

allow the heater to contact any potentially ammable materials, such as plastic racks or the sides of plastic tanks, as a re hazard may result.

 3.2eReservoirLiquidLevel

Fill the reservoir with the appropriate buffer. On circulating baths, the liquid level should be sufcient to cover the

heating coils, cooling coils (if applicable), pump, over-temperature sensor, and at least one inch (25mm) of the

temperature sensor. The level should be at the ll line indicator on the tank.

After lling the reservoir with uid, you must set the Safety Set and the Limit High value as well as your desired

control set point temperature.

3.2f External Temperature Probe

The Programmable Controller is designed to accommodate an optional remote temperature probe. The probe attaches to the 9-pin male D-connector on the rear panel of the Controller.

NOTE: On Digital Controllers, this connection is present, but not functional.

The Controller will automatically sense the presence of the external probe when main power (rear panel circuit breaker/power switch) is turned On. To control temperature using the external probe, “External” must be selected via the Controller’s software. You must also set a Maximum Setpoint Differential value.

To attain better temperature uniformity when using the external temperature probe in a jacketed or air-lled vessel, stirring the external uid with pumps or mixing air with fans is recommended. Expect only ±1.0°C stability with air or

any medium that does not conduct heat well. Insulate and cover the entire set-up to remove temperature gradients; the Controller cannot compensate for external chamber or component temperature gradients.

3.2g RS232 Interface

Programmable and Digital Controllers incorporate an RS232 interface to provide remote data-logging and control capability. Remote control capability is available on the Programmable Controllers only. The 9-pin female RS232

connector is located on the rear panel of the Controller.

The RS232 interface should be connected to a serial communication port on a remote PC using an appropriate cable.

Communications software compatible with Microsoft® Excel and National Instruments LabView™ are provided with

Programmable Controllers. Contact Technical Service at C.B.S. Scientic technicalservice@cbssci.com

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2h Power

An IEC power cord is provided with the Circulator. This power cord should be plugged into the IEC receptacle on the rear of the Controller and then plugged into a properly grounded outlet. Make sure that the power outlet is the same

voltage and frequency indicated on the identication label on the back of the Controller.

The use of an extension cord is not recommended. However, if one is necessary, it must be properly grounded and

capable of handling the total wattage of the unit. The extension cord must not cause more than a 10% drop in voltage

to the Circulator.

Once the unit has been connected to an appropriate electrical outlet, place the Circuit Breaker/Power Switch on the rear of the Controller in the ON position. The unit will run through a self-test.

The rst time power is applied to the Controller, the following display will appear. This display allows you to select the language that will be used for all subsequent displays. The instructions for selecting a language will be briey

displayed in each available language.

NOTE: The language selection display only appears the rst time the Controller is powered up.

When the language selection display appears, rotate the Select/Set Knob until the desired language is highlighted and then press the Select/Set Knob. The Controller will continue with the start-up sequence and then display “Standby” on the LCD.

DO NOT place the Power Switch on the front of the Controller ON until the Safety Set has been adjusted to the desired temperature.

3.2i Setting the Safety Set Point

The Safety Set feature automatically disconnects Controller power to the heater and pump in the event that the reservoir liquid level drops too low or the sensed temperature exceeds the Safety Set temperature. The Safety Set is user-adjustable between approximately 40° and 210°C. It should be set at least 5°C higher than the desired bath temperature.

Use a at blade screwdriver to rotate the Safety Set Indicator Knob to the desired temperature. Do not force the knob beyond the stops at either end of the temperature value range.

If the Safety Set temperature is exceeded during normal operation, a fault message will ash on the display and power to the heater and pump will be disrupted. To reset the fault, correct the problem (low liquid

level, incorrect Safety Set temperature, etc.), press the Safety Set Reset Button, and then the ESC Button. Normal

operation will resume.

3.2j Power On

Once the Safety Set temperature has been set, turn power to the Controller ON by pressing the Power Switch on the

front of the Controller. The following message will appear briey on the display:

3.2 Heater/Stirrer Set-Up and Programming – continued

The pump will begin operating; the display will show the current bath temperature and the set point temperature (Setpoint). If an external probe (Programmable Controllers only) is connected, both the internal and external bath temperatures will be displayed. The temperature probe selected to control bath temperature (internal or external) will be displayed in larger numerals.

NOTE: If the external temperature probe is selected to control bath temperature, but the temperature difference between the set point temperature and the internal bath temperature exceeds the Maximum Setpoint Differential setting, the heating rate will be controlled using the internal bath temperature until the set point/internal bath temperature difference is within the Maximum Setpoint Differential value. The word Internal will be highlighted on the display to indicate that the internal sensor is controlling bath temperature.

3.2k Local Lockout

This enables the user to lock all controls on the controller. While the feature is activated, the unit will remain running at the current settings.

To activate the local lockout feature, press and hold the Select/Set Knob for 10 seconds. Once locked, “LocalLock” will appear in the upper left corner of the display. The controller menus may still be viewed when Local Lockout is active, but no changes may be made.

Press and hold the Select/Set Knob again for 10 seconds to unlock the controls. Once unlocked, “LocalLock” will

disappear and the menu settings can once again be modied.

LocalLock

3.2l Setting the Bath Temperature Set Point

Press and release the Select/Set Knob. The “whole” numbers in the set point temperature will be

highlighted. Rotate the Select/Set Knob clockwise to increase the displayed value; rotate the knob

counter-clockwise to decrease the displayed value.

Press the Select/Set Knob to accept the new value. The decimal value in the set point temperature digits

will be highlighted. Rotate the Select/Set Knob clockwise to increase the displayed value; rotate the knob

counter-clockwise to decrease the displayed value. Press the Select /Set Knob to accept the new value.

NOTE: Increasing/decreasing the decimal value past “0” will cause a corresponding change in the “whole” number value. For example, if the current set point value is 24.8 and the desired value is 25.2, it is not necessary to change the 24 to a 25; increasing the decimal value from .8 to .2 will automatically increase the 24 to 25.

The Controller will not allow you to enter a set point value above the Limit High setting or below the Limit Low setting. Should you attempt to do so, the set point value will stop increasing/decreasing when the Limit value is reached and a Warning message will appear on the display. You must either change the set

point or change the Limit value. Reference the Heater/Stirrer instruction manual for detailed information.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2m Menu Navigation

Main Menu — To view the Main Menu items, rotate the Select/Set Knob. Continue turning the Select/Set Knob to scroll through the Main Menu displays.

To begin programming or view the options available in a Main Menu item, press the Select/Set Knob. The rst

available sub-menu item (or the last item in that sub-menu which was selected) will be highlighted like

To de-select an item, press the ESC Button. To return to the main operational display, press the ESC Button a second time or allow the display to timeout.

Sub-Menu Selections — To select an item in a sub-menu, press the Select/Set Knob. The rst available item (or the

last item in that sub-menu which was selected) will be highlighted. Rotate the Select/Set Knob clockwise to advance

to the next sub-menu item; rotate the knob counter-clockwise to go back to the previous item. Press the Select/Set Knob to select the highlighted item; the highlighting will change from to , indicating that the displayed value or choice may be changed.

Entering and/or Changing Sub-Menu Values — Once the desired sub-menu item has been selected (as described above), the displayed value is changed by rotating the Select/Set Knob. The change is accepted by pressing the Select/Set Knob. On sub-menu items requiring multiple entries, such as hours/minutes/seconds, an underline will appear under the rst value in that sequence which can be changed. (Example: 01:23:00 ) To accept the displayed value and/or advance to the next value in the sequence, press the Select/Set Knob. To return to the previous cursor position, such as from minutes back to hours, press the ESC Button. Once a value has been entered and accepted, the highlighting box around the value will disappear. If you do not wish to accept the displayed value, press the ESC Button or allow the display to timeout before pressing the Select/Set Knob.

THIS

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2n Menu Structure

Main Menu Item

Timer

Program

Run Program

Preferences

Limits / Alarms

Associated Sub-Menu Items

Set Beep

Program # Program Steps Program Loops Step # Step Setpoint Minutes/Seconds

View Prole

Program # Program Status

Readout

Units Sound Language Program

Limit High Alarm High Alarm Low Limit Low

Choices / Ranges / Comments

00:00:00 to 99:59:59 On or Off

1 to 10 1 to 50 1 to 99 1 to 50 –50° to 200°C (-50° to 392°F) 0 second to 999 minutes, 59 seconds

Displays temperature prole of program.

1 to 10

Start, Starting, Running, Paused, or Completed

#, #.#, or #.## (0, 1, or 2 decimal places) °C or °F 1 to 100 English / French / German / Spanish Time / Temperature

-50° to 200°C (-58° to 392°F)

Pump/AutoTune

Temperature Trend

Probe

Instrument

Pump speed AutoTune

No sub-menu; displays

temperature prole

Internal / External Maximum Setpoint

Differential

Contrast Timeout

Baud Rate

Maximum / Fast / Medium / Slow / Minimum Displays status of AutoTune procedure

2 minutes to 48 hours

Probe currently selected is shown Only displayed when External is selected

1° to 10°C range

00 to 30 5 to 60 seconds 110 / 300 / 600 / 1200 / 2400 /4800 / 9600 /

14400 / 19200 / 38400 / 57600

1. These Main Menu items are present on Programmable Controllers only.

2. This Main Menu item is present on Refrigerating/Heating Circulators only.

NOTE: There are additional displays after “Instrument “ in the Main Menu. However, there are no user-settable functions on these displays.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2o Fluid Type

Located in the Fluid Type screen are two adjustable parameters: Specic Heat and Volume. By adjusting the Specic Heat you will be optimizing the circulator’s temperature control based on the uid being used. The table below lists a few common uids. You can also refer to the MSDS sheets for your specic uid.

The other parameter, Volume, is strictly for diagnostic purposes and does not affect the performance of the unit.

When the Specic Heat of the uid and the Volume are entered correctly, the circulator will display the amount of

energy the unit is putting into the system (in Watts). When the unit is stable this will display 0+/-25 Watts.

FLUID DESCRIPTION SPECIFIC HEAT @25ºC

Distilled Water 1.00

Ethylene Glycol 30% / Water 70% 0.90

Ethylene Glycol 50% / Water 50% 0.82

3.2p Setting Operational Parameters and Functions

All operational parameters and functions are programmed and controlled via the Controller’s software settings. Most are user-adjustable and easily accessed via the Main Menu. The Main Menu is accessed by rotating the Select/Set Knob. A particular Main Menu item is selected by pressing the Select/Set Knob when that item is highlighted.

3.2qSettingandStartingtheTimer

The Timer sub-menu allows you to program the Controller’s timer to alert you once a specic period of time has

elapsed. It should be used as you would an external timer.

IMPORTANT: The Timer is independent of temperature control. It does not start or stop heating/cooling. The

Controller continues maintaining temperature at the set point even though the designated time period has elapsed.

To set the timer, access Timer on the Main Menu, select Set, and then enter the desired period of time. The timer’s audible signal can be turned On and Off via the selection named Beep on the Timer sub-menu.

To start the timer, press the Timer button on the Controller’s front panel. A timer icon and Beep icon (indicating either On – • or Off – the icon with an X through it), along with a countdown timer, will appear on the bottom of the LCD. The Timer LED will light continuously.

Once the designated time period has elapsed, the audible signal (if enabled) will sound and the countdown timer will

display the amount of time which has elapsed since the designated time period ended. The timer LED will ash.

To silence the audible signal and/or clear the timer display from the LCD, press the Timer Button.

The timer may also be paused at any time during the countdown period by pressing the Timer Button. When this

occurs, the Timer LED will ash and the word “Paused” will appear on the display adjacent to the countdown timer.

.2 Heater/Stirrer Set-Up and Programming – continued

3.2r Setting Preferences

The Preferences sub-menu allows you program global preferences regarding instrument operation.

Readout — This is the number of decimal places to which temperatures will be displayed (0, 1, or 2).

Units — This is the unit in which temperatures will be displayed (°C or °F)

Sound — This is the volume level for the unit’s audible signal. When it is selected, the volume of the audible signal changes as the Select/Set Knob is rotated. You must press the Select/Set Knob to accept the displayed volume value; if you press the ESC Button or allow the display to timeout without pressing the Select/Set Knob, the sound level will remain where it was previously set.

Language — This is the language used for displays. When this is selected, a sub-menu appears with the available languages.

Program — This menu item appears only on the Programmable Controller. It is used to select whether programs are run using Time or Temperature as the priority.

When Time is selected, the program begins running when the bath temperature reaches the programmed set point for step one and continues until the total programmed period of time has elapsed. The set point target for any given step (except step one) may or may not be reached before the program advances to the next step.

When Temperature is selected as the priority, the program begins running when the bath temperature reaches the programmed set point for step one. Each subsequent step runs until the programmed set point for that step is reached, regardless of how much time has elapsed.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2s Setting High/Low Temperature and Alarms

The Limits/Alarms sub-menu allows you to establish temperatures at which either power to the temperature control components (heater/condenser) will be disconnected (Limits) or which Controller’s audible alarm will sound (Alarms).

Limit High Temperature — This feature provides additional safety and protection by allowing a selectable upper temperature limit set point. To avoid an unwanted shutdown during regular operation, the high limit value should be set at least 5°C higher than the selected control temperature. It should never be set higher than the Safety Set Set Point temperature

If you attempt to enter a set point value that exceeds the Limit High value, the audible alarm will sound and a Warning

message will ash on the display when the Limit High value is reached. You will also be prevented from increasing

the set point value any further.

To clear a Limit High warning, enter a higher value for the Limit High or reduce the control temperature set point.

If the Limit High value is exceeded during operation (due to a Controller fault, excessive heat load, etc.), a Fault message will appear on the display and power to the heater and compressor will be disconnected. The pump will continue to run.

Alarm High Temperature — This feature is useful if you are using the bath to cool an external device. It alerts you

when bath temperature exceeds your programmed alarm high temperature setting (due to insufcient cooling,

blocked lines, etc.).

When the Alarm High value is exceeded, a Warning message ashes on this display and the audible alarm sounds.

Heater, compressor, and pump operation continue.

To clear an Alarm High warning, correct the problem or increase the Alarm High temperature value.

Alarm Low Temperature — This feature is useful if you are using the bath to warm an external device or need to maintain the bath at a minimum temperature. It alerts you when bath temperature falls below your programmed alarm low temperature setting.

When bath temperature falls below the Alarm Low value, a warning message ashes on the display and the audible

alarm sounds. Heater, condenser, and pump operation continue.

3.2 Heater/Stirrer Set-Up and Programming – continued

To clear an Alarm Low warning, correct the problem or decrease the Alarm Low temperature value.

Limit Low Temperature — This feature provides additional safety and protection by allowing a selectable lower temperature limit set point.

If you attempt to enter a set point value that exceeds the Limit Low value, the audible alarm will sound and a Warning

message will ash on the display when the Limit Low value is reached. You will also be prevented from decreasing

the set point value any further.

To clear a Limit Low warning, enter a lower value for the Limit High or increase the control temperature set point.

If the Limit Low value is exceeded during operation (due to a Controller fault, excessive cooling load, etc.), a Fault message will appear on the display and power to the heater and condenser will be disconnected. The pump will continue to run.

Limits/Alarms Example

Initial Bath Temperature = 20°C Bath Temperature Set Point = 37°C Limit High Temperature = 60°C Alarm High Temperature = 40°C Alarm Low Temperature = 35°C Limit Low Temperature = 11°C

Limit High Set Point. Bath set point temperature cannot be raised above this value.

High Temperature Alarm Set Point. Alarm activates if bath temperature rises to this value.

Degrees

Low Temperature Alarm disabled

until bath temperature rises above Low Temperature Alarm set point value.

10 20 30 40 50 60 70 80 90

Limit Low Set Point. Bath set point temperature cannot be lowered below this value.

3.2t Selecting the Pump Speed

If bath temperature moves above

or below Limit values, alarm activates and power to the heater and condenser is disconnected.

Low Temperature Alarm Set Point. Alarm activates if bath

temperature falls to this value.

Minutes

Pump speed is selected from the Pump/AutoTune menu. This display shows the current pump speed setting. Five pump speed settings are available – Maximum, Fast, Medium, Slow, and Minimum. Select Minimum for DGGE applications.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2u Displaying the Bath Temperature Trend

The Controller can store up to 48 hours of bath temperature data. The data can be viewed by selecting Temperature Trend from the Main Menu.

To view the temperature trend data, rotate the Select/Set Knob until the Temperature Trend display appears, showing the most recent temperature data. The time period which the displayed trend line covers appears in the lower left corner of the display. It will range from two minutes to 48 hours.

To view a different period of time, press the Select/Set Knob and then rotate it until the desired time period appears.

The temperature trend display will not timeout. To return to the main operational screen, press the ESC Button.

NOTE: If main power is turned off or power is accidentally lost, temperature trend data will be lost. If the Controller is put in “Standby” (main power On, Controller power Off), the data will be retained.

3.2 Heater/Stirrer Set-Up and Programming – continued

3.2v Selecting the Temperature Probe (Internal or External)

The Probe sub-menu allows you to designate whether to control temperature using the internal bath temperature or

the uid temperature at an external device. It is available on the Programmable Controller only and requires the use

of an optional external temperature probe.

NOTE: If an external temperature probe is not connected to the Controller, only “Internal” will be available for selection. For information on connecting an external temperature probe.

When External is selected as the primary temperature probe, the Maximum Setpoint Differential setting becomes available.

This allows you to set the maximum allowable difference between the set point temperature and the internal bath temperature. It is intended as a safety feature to protect the internal bath from over-heating or over-cooling in the event that the external temperature control set point cannot be achieved. The Maximum Setpoint Differential value may be set from 1° to 10°C. The factory default value is 10°C.

Temperature Control / Display When Using an External Probe

When the difference between the set point temperature and internal bath temperature exceeds the programmed Maximum Setpoint Differential value, heating is controlled using the internal bath temperature. “Internal” is highlighted on the temperature display.

Control of the bath heating rate will be based on the internal bath temperature until the difference between the set point and the internal bath temperature is at the Maximum Setpoint Differential value. When the heating rate is being controlled using the external bath temperature, the word “External” is highlighted on the display.

NOTE: When the external temperature probe is in use, external bath temperature is displayed in large numerals, regardless of whether temperature control is based on the internal or external bath temperature.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.2 Heater/Stirrer Set-Up – continued

3.2w Setting the Display Contrast and Timeout

Display Contrast and Display Timeout appear as sub-menu items under Instrument in the Main Menu. These menu items allow you to change the readability of the LCD and set the length of time, which can pass without menu activity before the display will revert to the main operational display.

NOTE: When Contrast is selected, the display contrast will change as the Select/Set Knob is rotated. You must press the Select/Set Knob to accept the displayed contrast value; if you press the ESC Button or allow the display to timeout without pressing the Select/Set Knob, the display contrast value will remain where it was previously set.

3.2x Setting the Baud Rate

This sub-menu selection also appears under Instrument in the Main Menu. It allows you to set the baud rate at which

data will be transmitted over the RS232 interface.

3.3 Heater/Stirrer Programming

3.3.1 Setting Operational Parameters and Functions

1. Setting and Starting the Timer

The Timer sub-menu allows you to program the Controller’s timer to alert you once a specic period of time has

elapsed. It should be used as you would an external timer.

IMPORTANT: The Timer is independent of temperature control. It does not start or stop heating/cooling. The Controller

continues maintaining temperature at the set point even though the designated time period has elapsed.

Once the designated time period has elapsed, the audible signal (if enabled) will sound and the countdown timer will

display the amount of time which has elapsed since the designated time period ended. The timer LED will ash.

To silence the audible signal and/or clear the timer display from the LCD, press the Timer Button.

The timer may also be paused at any time during the countdown period by pressing the Timer Button. When this occurs,

the Timer LED will ash and the word “Paused” will appear on the display adjacent to the countdown timer.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.3 Heater/Stirrer Programming- con’t.

3.3.2 Entering/Modifying a Temperature Program

NOTE: This function is available on Programmable Controllers only. See Writing a Temperature Program below for

information on creating a time/temperature prole.

This menu selection allows you to program and store up to ten individual time/temperature proles. Each program can have up to 50 steps and 99 program loops. Once a program has been entered, any portion of it may be modied.

Program # — This is the identication number assigned to the program. It is used to select/run the program (see Running a Temperature Program below). You may enter a number from 1 to 10. If you enter a number that has been

assigned previously, any changes made overwrite the prior program.

Program Steps — This is the number of steps in the program. A program can have from 1 to 50 different steps.

NOTE: If you are modifying a program and change the number of steps (e.g., reduce the number of steps from 25 to 10), steps 11 through 25 will no longer appear. However, if you later increase the number of steps in that program (e.g., from 10 to 15), the original programming for steps 11 through 15 will reappear.

Program Loops — This is the number of times the program will run before stopping. A program may be repeated up to 99 times.

Step #, Set, MMM/SS — This is the temperature set point and time for the selected step in the program.

To enter the set point and time for the step, rotate the Select/Set Knob until a Step number is highlighted like THIS. Press the Select/Set Knob again; the highlighting will now look like THIS. Rotate the Select/Set Knob to scroll to the desired Step number and press the Select/Set Knob. The boxed highlighting will move to the temperature set point

eld associated with that step.

Rotate the Select/Set Knob until the desired temperature set point is displayed. You may advance the cursor (underline) to the next number in the set point eld by pressing the Select/Set Knob. Press the ESC Button to return

to the previous cursor position.

3.3 Heater/Stirrer Programming- con’t.

Once the temperature set point has been entered, press the Select/Set Knob to advance to the time (minutes/

seconds) eld. Time information is entered the same way as the temperature set point information.

When you press the Select/Set Knob to accept the time displayed time information, the highlighted box will

automatically advance to the set point temperature eld associated with the next step of the program.

IMPORTANT: The time eld establishes the amount of time the Controller should take to reach the temperature set

point for the next step (i.e., the ramp rate).

View Prole — This allows you to view the programmed time/temperature prole step-by-step. Rotate the Select/Set

Knob to move through the various steps in the program. When the cursor (a vertical line) reaches the beginning of a step, a message box will appear displaying the step number, set point, and time.

To return to the main operational display, press the ESC Button or allow the display to timeout.

Writing a Temperature Program

Programmable Controllers permit the user to create up to 10 different time/temperature programs, each of which can have as many as 50 steps and be repeated up to 99 times. The following information is intended to provide you with some guidelines for creating useful programs.

1. Circulating baths are designed primarily to hold temperatures constant rather than change temperatures rapidly.

Do not underestimate the amount of time the circulator needs to heat or cool a uid to a given temperature.

Larger baths or circulators being used in closed or open loops will need more time to reach a programmed set point.

2. Programs may be run using either a Time- or Temperature-based priority. If achieving successive temperature

set points is critical, Temperature should be selected as the priority. If completing a program in a xed amount of

time is essential, Time should be selected as the priority. See Section 5.12.4 – Preferences for more information.

3. If a program must run within a set period of time (Time priority), have the bath temperature at or very close to the initial set point before starting the program. The program will not start running until the set point temperature

programmed for the rst step is achieved.

4. To incorporate a “soak” period in the program, enter the same set point for two adjacent steps in the program.

The time duration programmed for the rst step should equal the desired “soak” period; the time duration for

the second step should be short (e.g., 1 second). The temperature set point for the last step in a program also

functions as an indenite “soak”. The Controller maintains temperature at the last programmed set point until a

new set point is entered.

5. The step time in a program establishes the ramp rate that will be used to reach the programmed set point for the next step. If you want to increase/decrease temperature slowly, set a lengthy step time. If you want to increase/ decrease as fast as possible, set a short step time. Keep the heating/cooling capabilities of your instrument

in mind, however. If you are running a program using Time-based priority, uid temperature may not reach a

desired set point temperature if the time allotted is too short.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.3 Heater/Stirrer Programming- con’t.

Programming Examples

Example A

Initial Bath Temperature = 25°C Program Priority = Temperature

Desired Profile: Cool bath temperature to 20°C and hold it there for 25 minutes. Increase bath temperature to 30°C and hold it there for 15 minutes. Increase bath temperature to 50°C over a 45 minutes period. Decrease bath temperature to 5°C and hold.

This example requires a 6-step program:

55 50 45 40 35 30 25 20

Degrees C

15 10

5 0

0 5 15 30 45 60 75 90 105 120 135 150 165

Step 2

Step

Step 4

Step 1

Step

Step 3

Step

Step 5

Step

Step 6

Step

Minutes

Program

Step

1 20°C 25 minutes

2 20°C 1 second

3 30°C 15 minutes Fluid temperature is maintained at 30°C for 15 minutes.

4 30°C 45 minutes

5 50°C 1 second

6 5°C 1 second

Step Set

Point

Step

Duration

Controller Operation

Controller cools fluid to 20°C as fast as possible. Until 20°C temperature set point is achieved, “Starting” appears on the display. When fluid temperature reaches 20°C, “Running” appears on the display.

Fluid temperature maintained at 20°C for 25 minutes.

Controller heats fluid as fast as possible until the 30°C set point programmed for Step 3 is reached.

Controller slowly heats fluid until 50°C set point programmed for Step 5 is reached. Ramp rate is based on the 45 minute step duration.

Controller then cools fluid as fast as possible until 5°C set point programmed for Step 6 is reached.

“Complete” appears on display. 5°C fluid temperature is maintained until set point is changed.

3.3 Heater/Stirrer Programming- con’t.

Example B

Initial Bath Temperature = 25°C Program Priority = Temperature

Desired Profile: Cool bath temperature to 20°C and hold it there for 10 minutes. Decrease bath temperature to 10°C over 15 minutes. Hold bath temperature at 10°C for 15 minutes. Increase bath temperature to 50°C over a 1-hour period.

This example requires a 5-step program:

55 50 45 40 35

Step 1

Step

30 25 20

Degrees C

Step

15 10

Step

Step 2

0 5 15 30 45 60 75 90 105 120 135 150 165

Step 3

Step 4

Step

Step 5

Step

Minutes

Program

Step

1 20°C 10 minutes

2 20°C 15 minutes

3 10°C 15 minutes Fluid temperature is maintained at 10°C for 15 minutes.

4 10°C 1 hour

5 50°C 1 second

Step Set

Point

Step

Duration

Controller Operation

Fluid temperature is maintained at 20°C for 10 minutes.

Controller cools fluid to 10°C set point programmed for Step 3. Ramp rate is based on the 15 minute step duration.

Controller slowly heats fluid until 50°C set point programmed for Step 5 is reached. Ramp rate is based on the 1 hour step duration.

“Complete” appears on the display. 50°C fluid temperature is maintained until set point is changed.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.3 Heater/Stirrer Programming- con’t.

Example C

Initial Bath Temperature = 25°C Program Priority = Temperature

Desired Profile: Cool bath temperature to 20°C as fast as possible. Increase bath temperature to 55°C as fast as possible. Repeat 7 times.

This example requires a 2-step program with the number of loops set to 8:

60 55 50 45 40 35 30 25

Degrees C

20 15

Step 1

Step

5 0

0 5 15 30 45 60 75 90 105 120 135 150 165

Program

Step

1 20°C 1 second

2 55°C 1 second

Step Set

Point

Step

Step 2

Step

Duration

Minute

Controller Operation

Controller cools fluid to 20°C as fast as possible. Until 20°C temperature set point is achieved, “Starting” appears on the display.

When fluid temperature reaches 20°C, “Running” appears on the display.

Controller applies heat until 55°C set point for Step 2 is reached.

Controller loops back to Step 1, applying cooling until 20°C set point is reached.

Steps 1 and 2 repeat seven more times. When the last loop has been completed, “Complete” appears on the display. Fluid temperature is maintained at 55°C until the set point is changed.

3.3 Heater/Stirrer Programming- con’t.

3.3.3 Running a Temperature Program

NOTE: This function is available on Programmable Controllers only.

The Programmable Controller can store up to 10 user-dened time/temperature programs, which can later be run with

just a few simple commands. See Entering/Modifying a Temperature Program above for more information.

Programs may be run using either a Time- or Temperature-based priority. This priority is selected under Program in the Preferences menu (see Setting Preferences below).

When Time is used, the program begins running when the bath temperature reaches the programmed set point for step one. It continues running until the total programmed length of time for all steps has elapsed, regardless of whether the set point temperatures for steps two and above have been achieved.

When Temperature is selected as the priority, the program begins running when the bath temperature reaches the programmed set point for step one. Each subsequent step is run until the programmed set point for that step is reached, regardless of how much time has elapsed.

Selecting a Program— To select a temperature program, rotate the Select/Set Knob until the Run Program menu appears and then press the Select/Set Knob.

If the Program # eld is highlighted as shown above, press the Select/Set Knob and then rotate the Select/Set Knob

until the number of the program you wish to run is displayed. Press the Select/Set Knob a second time to accept the displayed program number. If the word Start is highlighted, rotate the Select/Set Knob one click counter-clockwise to highlight the program number.

Running a Program — Once you have selected and accepted the program number, rotate the Select/Set Knob until Start is highlighted. Press the Select/Set Knob; the program will automatically begin running. The word “Starting” will

appear at the lower left of the Run Program menu and will remain there until the bath temperature reaches the set point programmed for step one. It will then be replaced by the word “Running.”

While a program is running, the Run Program and main operational displays will alternate on the LCD. The Run

Program display shows the current step number, the target set point for the next step, time at the current step, loop number, program status, and total elapsed time. The main operational display shows bath temperature, set point, and program status.

Pausing or Stopping a Program — A program that is running may be paused or stopped at any time. To do so, press the Select/Set Knob until Starting or Running is highlighted and then press the Select/Set Knob again. Rotate the Select/ Set Knob until the desired function (Pause / Stop) is highlighted and then press the Select/Set Knob. If you do not wish to pause or stop the program, select and enter Escape.

If the program has been paused, “Paused” will appear on the lower left of the display. If the program has been stopped, “Start” will appear on the lower left of the display.

To resume running a program that has been paused, press the Select/Set Knob, select Resume, and then press the

Select/Set Knob a second time. The program will resume operation from the point of disruption. Select Stop if you wish to stop the program or Escape if you want to keep the program paused.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.3 Heater/Stirrer Programming- con’t.

If a program is stopped or paused, the Controller will control temperature using the set point value that was active when the program was interrupted.

NOTE: If you select the Temperature Trend display while running a program, that display will remain on screen until the ESC Button is pressed.

End of Program — Once the selected program has run, “Completed” will appear in the lower left of the Run Program

display. The Controller will keep the bath liquid at the last temperature set point until a new program is started or Run

Program has been exited and a new set point entered.

Exiting Run Program — Once a program has been completed, “Completed” will appear at the lower left of the Run

Program sub-menu. Highlight “Completed” and then press the Select/Set Knob. “Start” will appear. You may now return to manual set point control, run another program, or turn Controller power Off.

Loss of Power — If the Controller is placed in Standby (front panel power turned Off), the Controller will resume running the program when Controller power is restored. If main power (rear panel circuit breaker/power switch) is turned Off or electrical power is lost while a program is running, paused, or completed (but not exited), the appropriate Fault message will be displayed upon restoration of power (see Section 5.13 – Controller Messages). Press the ESC Button to clear the Fault message; the Controller will resume operation at the set point at which power was lost. If the program was running or paused, it will not resume. If it was completed, it must be exited before a new program can be run.

3.3.4 Setting Preferences

The Preferences sub-menu allows you program global preferences regarding instrument operation.

Readout: This is the number of decimal places to which temperatures will be displayed (0, 1, 2, or 3).

Units: This is the unit in which temperatures will be displayed (°C or °F)

Sound: This is the volume level for the unit’s audible signal. When it is selected, the volume of the audible signal

changes as the Select/Set Knob is rotated. You must press the Select/Set Knob to accept the displayed volume value; if you press the ESC Button or allow the display to timeout without pressing the Select/Set Knob, the sound level will remain where it was previously set.

Language: This is the language used for displays. When this is selected, a sub-menu appears with the available languages.

Program: This menu item appears only on the Programmable Controller. It is used to select whether programs are run using Time or Temperature as the priority.

When Temperature is selected as the priority, the program begins running when the bath temperature reaches within ±0.1°C of the programmed set point for step one. Each subsequent step runs until the programmed set point for that step is reached, within ±0.1°C, regardless of how much time has elapsed.

Display Temperature Filter: This sets the rate at which the temperature display is updated. The default setting is 4.

Higher settings will result in less display uctuation; a setting of 0 displays real-time temperature probe data. The display temperature lter can be set from 0 to 60.

3.3 Heater/Stirrer Programming – continued

3.3.5 Controller Messages

Message Display Description ActionRequired

Standby mode

An attempt has been made to set the temperature set point higher than the Limit High setting

Fluid temperature is higher than the Alarm High setting

Fluid temperature is lower than the Alarm Low setting

An attempt has been made to set the temperature set point lower than the Limit Low setting

Safety Set temperature exceeded

Normal — Indicates that the Circuit Breaker/Power Switch is ON and the Controller Power Switch is OFF

Error — Decrease temperature set point or increase Limit High setting

High Temperature Warning — Decrease temperature set point, increase Alarm High setting, or correct condition

causing high uid temperature. Pump, heater, and

compressor operation continue.

Low Temperature Warning— Increase temperature set point, decrease Alarm Low setting, or correct condition

causing low uid temperature. Pump, heater, and

compressor operation continue.

Error — Increase temperature set point or decrease Limit Low setting

Safety Fault — Power to heater, compressor, and pump automatically disconnected. Correct problem and then

press Safety Set Reset Button and ESC Button to clear

fault message and restore operation.

www.cbsscientic.com

Fluid temperature is higher than the Limit High setting

Fluid temperature is lower than the Limit Low setting

Indicates main power was lost while a program was running

Indicates main power was lost while a program paused

Indicates main power was lost after a program was completed, but before it was exited

Safety Fault — Power to heater and compressor

automatically disconnected. Decrease uid temperature

or increase Limit High setting. Pump will continue to run.

Safety Fault — Power to heater and compressor

automatically disconnected. Increase uid temperature or

decrease Limit High setting. Pump will continue to run.

Error — Program must be restarted. Press ESC Button to clear Fault message.

Error — Press ESC Button to clear Fault message and then exit the program.

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

Message Display Description ActionRequired

Analog to Digital conversion fault

Internal temperature probe fault

External temperature probe fault

Internal error

Instrument Failure — Power to heater, compressor, and pump automatically disconnected. Contact supplier.

Probe Failure — Power to heater, compressor, and pump automatically disconnected. Contact supplier.

Probe Failure — Power to heater, compressor, and

pump automatically disconnected. Replace external

temperature probe or operate instrument using internal temperature probe. Contact supplier if fault persists.

Probe Failure — Power to heater, compressor, and

pump automatically disconnected. Replace external

temperature probe or operate instrument using internal temperature probe. Contact supplier if fault persists.

Error — Press ESC Button to continue operation. Contact supplier.

Triac fault

Internal memory fault

Triac Failure — Power to heater, compressor, and pump automatically disconnected. Contact supplier.

Failure — Power to heater, compressor, and pump automatically disconnected. Contact supplier.

3.4 Running the Heater/Stirrer

1) Fill reservoir with at least 23 Liters of buffer. (refer to section 3.1 for working buffer volume).

2) Turn Heater/Stirrer “On” (Fig 3-3). Reference section 3.2l Setting the Bath Temperature Set Point (page

15). a) For DGGE, set at 60ºC. b) For TGGE, select temperature ramp program, see section 4.3. c) SSCP may require tank buffer to be below ambient temperature (20-25ºC). (references 13 & 14) i) Attach external chiller to inlet and outlet of cooling coil (located behind heater/stirrer control). See page 8.

ii) Set chiller temperature 2-5ºC BELOW desired temperature of tank buffer. Run chiller until tank

buffer reaches temperature. iii) Set DTSK heater/stirrer temperature at desired running temperature. Heater/stirrer will control

nal tank temperature. NOTE: Autotune heater/stirrer with chiller coolant circulating through cooling coil: Refer to section

3.2r. Conrm pump speed is 12% after autotune.

Fig. 3-3

3.5 Preparation/Cleaning of Glass Plate for Gel Casting

Hand wash both plates with a high quality lab detergent followed by a complete rinsing with dH2O. Air dry or use a

lint-free tissue. Spray/wipe the chosen inner surfaces of the plate set with 95% ethanol and dry with lint-free tissue.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.6GelCastingTechniques.

A. Gel Wrap™ Gasket Casting Method.

B. Vertical gradient gel casting using GM-40 gradient maker and gravity ow.

C. Vertical gradient gel casting using GM-40 gradient maker and a Mini-pump. D. Vertical gradient gel casting using GM-40 gradient maker, Mini-pump and Multi-gel Caster.

A. Gel Casting using Gel Wrap Gasket Casting method

For Vertical DGGE, TTGE or SSCP, use the set of spacers which do not have the small hole or channel milled into the lower end, these are for casting perpendicular gels.

1. Start by holding the rectangular back plate with the rounded bottom

corners and start applying the gasket around one side of the glass

plate. NOTE: one side of the “U” shaped gasket is at and the other

side has tubing that will act as a seal around the spacers.

2. When applying the gasket over the rounded corners of the back

glass plate,make sure the notches on the gasket align with the rounded corners of the glass plate. Once the gasket is pushed over the bottom edge and corners, work it down the remaining side.

3. Place the gasketed plate on the lab bench with the tubing side up,

and extend the bottom of the plate over the edge of the bench, approximately ¾ of an inch. Place the spacers along side the inside edges of the gasket. Be sure the rounded corner end of each spacer is facing the outside bottom of the plate, following the radius of the glass.

4. Place the notched plate on top of the bottom assembly, starting from

the bottom edge and gently easing the plate down. Verify the gasket is smooth around the edges and then clamp along the bottom.

5. Lift the assembly and stand it on the base of the clamps. For leveling,

push glass plate assembly down until it stops against clamp body. Clamp the sides of the assembly with additional casting clamps on either side. As each clamp is attached, be sure the gasket is aligned between the plates forming a seal. The glass plate assembly is ready to receive the acrylamide solution, sec. 3.7.

6. Go to section 3.8 and follow instructions under the heading “Preparation of the Cassettes”.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.6GelCastingTechniques-continued

C-1

C-2

V-1

V-2

B.VerticalgradientgelcastingusingGM-40gradientmakerandgravityowforDGGE.

1. Place the GM-40, gradient maker, on an elevated magnetic stirrer with a small “ea” stir bar in

the cylinder (C-2) closest to the outlet. The gradient maker should be tted with a luer valve, (V-

2) and a 20ga needle with attached tubing to deliver acrylamide to the gel plate sandwich. (Fig 3-4). With the height differential between the luer valve (V-2) and the top of the gel plate sandwich

between 2-4 inches, it takes approximately 5-9 minutes to cast the gel. Raising the height of the gradient maker can increase ow rate. Flow can be regulated by turning luer valve (V-2). Flow

rate appropriate for each gradient should be determined empirically.

Fig. 3-4

2. For TTGE and SSCP single percentage gels, use a pipette or syringe to inject polyacrylamide into

glass plate cassette.

 Refertosection3.7forGradientGelFormulaProtocol.

See section 4.3 for running conditions.

3.6GelCastingTechniques-continued

C. Vertical gradient gel casting using gradient maker (GM-40) and a mini-pump for DGGE.

1. Alternatively, you may choose to use a “mini-pump” or other peristaltic pump to cast gels as shown in (Fig 3-5). If so, secure the gradient maker to a ring stand and connect the outlet tubing to the mini-pump tubing adapter. Connect tubing from mini-pump to a 20ga. needle for

afxing between glass plates.

Fig. 3-5

2. For TTGE and SSCP single percentage gels, replace the gradient maker with ask (more

consistent than syringe).

  Refertosection3.7forGradientGelFormulaProtocol.

See section 4.3 for running conditions.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

separation sheet

notched glass plate

spacer

unnotched glass plate

shim sheet

front panel with

captive thumbscrews

casting chamber

lid (used to tilt

assembly)

separation sheet

D. Vertical gradient gel casting using GM-40 gradient maker, Mini-Pump and Multi-Gel Caster for DGGE.

1) Multi-Gel Caster Assembly

a) Clean the gel caster with soap and water and the glass plates with alcohol.

b) Place lid on bench and put the top end of the casting chamber (locating groove provided) on

the lid so that it rests at an angle with the gasket facing up.

c) Start by stacking one separation sheet, followed by unnotched glass plate, spacer set, then

notched glass plate. Repeat this sequence until all 4 sets (or 10 sets) are neatly stacked into the casting chamber. Place one additional separation sheet on the last glass plate. See gure 1.

d) Place front panel on chamber and tighten the 5 captive thumbscrews. With 4 (or 10) sets of

e) Bring assembled caster to the vertical position. Test the tightness of the assembly by pulling

gure 1.

glass plates with 0.75mm spacers, the front panel seals against the gasket and compresses the glass sandwiches. Using fewer than 4 (or 10) glass plate sets at 0.75mm thick will require the use of the enclosed shim sheets to achieve a tight seal and light compression of the glass plate sets. Use of other than 0.75mm thickness spacers may require the use of additional shim sheets, which must be ordered separately.

one of the polycarbonate shim sheets. If it comes out easily, use extra shims to increase tightness against the front panel.

Mini-Pump

Magnetic Stirrer

Gradient Maker

Multi-Gel Caster

Stopcock SC-2001

Silicone Tubing

f) Set up components as shown below in gure 3-6.

Fig.3-6

g) For TTGE and SSCP single percentage gels, replace the gradient maker with ask.

h) For gradient gels connect a gradient former to a small peristaltic pump (MPP-100), and the

i) Carefully insert combs into glass plate sandwiches. Place lid on top of chamber until gels

Refertosection3.7forGradientGelFormulaProtocol.

See section 4.3 for running conditions.

2) Disassembly

a) Remove lid and carefully remove one comb to ensure polymerization is complete. Return

pump outlet to the luer valve mounted on the front panel. Fill caster to within 0.5cm of the glass plate notches, turn pump off then close luer valve.

polymerize.

caster to horizontal angle position as shown in gure 1. Disassemble the unit by unscrewing thumbscrews. Release each gel sandwich by pulling the polycarbonate separation sheet.

If you are planning to store some of the gels for later use, leave the comb in place, rinse excess acrylamide from glass plates, and wrap in Saran wrap or store in Zip-Lock bags in the refrigerator.

b) Wash all Multi-Caster components after each use with mild detergent and rinse with DI water.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.7 Vertical Gradient Formation for DGGE.

To determine the range of gradient appropriate for your fragment analysis, please read the

enclosed paper by Myers, Shefeld and Cox, especially section 6.1.3 through 6.2.1 (pages 124

to 126). This gives you an excellent overview of the determination of melting behavior of your fragments.

1. The following is a typical protocol for casting a 40%-60% denaturing gradient gel. See section 4.2 for stock solutions. Refer to Section 3.5 Vertical Gradient Casting for apparatus assembly. In an

ice bucket, place two 50ml conical tubes labeled “A” and “B”. Add to tube “A”

• 11.5ml of 40%/7.5%

• 80 µI (10%) APS

• 5 µl TEMED

2. Add to tube “B”:

• 11.5ml of 60%/7.5%

• 80 µI (10%) APS

• 5 µI TEMED

3. Pour solution "B" into right side of gradient maker (C-2),(GM-40) (Fig. 3-5), and open interior valve to allow air bubble to escape. Let as much as 1 ml "B" solution BACKFLOW into left side of

gradient maker. Decant the 1 ml back into right side with pasteur pipette. Remove any residual

solution from (C-1) with absorbent paper.

4. Add solution "A" to left side of gradient maker.

5. Turn on magnetic stirrer.

6. Exit tube with needle should be secured to gel plate notch with tape.

7. Open inside valve rst (V-1), then outside (V-2) valve to start gravity ow. Optionally, Turn on

mini-pump and pump acrylamide at medium speed. Determine optimal speed empirically.

8. Gel volume is 23ml, using the 0.75mm spacers. If gel volume is not enough to ll sandwich, use 0% to "top-off". If using water saturated butanol for overlay, leave 0.5cm void to create at

interface.

9. Insert the 16-well rectangular tooth comb. Allow the gel to polymerize for 20-30 minutes.

10. If using Multi-Gel Casting Chamber please refer to instructions at bottom of page 33 under Disassembly.

3.8 Preparation of the Cassettes

1. After polymerization rinse gel plate assembly with D.I. water to remove excess acrylamide or denaturants from plate exterior.

2. Remove comb and quickly transfer gel sandwich to cassette by removing all #2 clamps.

LEAVE GEL WRAP IN PLACE. The Gel Wrap acts as a barrier and prevents perpendicular

electrical elds from interfering with outside lanes. Re-clamp to cassette using #1 clamps/ 2

per side. Dislodge entire bottom portion of the Gel Wrap by pulling from the plates to allow buffer contact with the gel. (Fig 3-10)

3. To avoid capturing a bubble under gel, immerse cassette at an angle into pre-heated buffer

tank (Fig.3-7). If a bubble is captured or forms under the gel, expel using a buffer-lled syringe tted with a bent 16 gauge needle. Attach buffer recirculating tubing to each upper reservoir (Fig. 3.8 & Fig 3-9). Rinse each well with buffer to remove non-polymerized acrylamide or excess urea and ll upper reservoir with buffer.

dislodge entire bottom portion of

the Gel Wrap™

Fig. 3-7

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.8 Buffer Cycling Connections

Buffer Cycling Connections-

Dual Cassette

Fig. 3-8

Fig. 3-9

1. Attach small bore tubing to barb tting on cassettes. (Fig 3-9)

2. Turn heater/stirrer on to ll upper reservoir; begin buffer cycling.

3.9 Running the Gels

1. Load samples at 1:1 with neutral dye. Load 5-10 mg Genomic DNA/well or 1-2 mg cloned (B-globin)/well. Determine concentration by O.D. 260.

2. Attach black power leads to cassettes

3. Close lid to engage safety interlock. Turn on power supply to 150V (40mA/one dual cassette) constant Volts for 5-7 hours.

Fig. 3-10

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.10 Removing the Gels

1. When run is completed, turn off power supply, disconnect recirculating tubes and power leads, remove cassettes from tank and unclamp glass sandwiches from cassettes.

Remove Gel Wrap™ by pulling away from plate.

2. Using a wedge plate separator,cat. # WPS-100, pry plates apart and immerse gel/plate in buffer tray. Stain with EtBr (0.5g/ml) for 5-10 minutes. Lift gel on plate out of tray and rinse with ddH20. Flip over onto Saran® wrap and peal off glass plate. View bands on transilluminator. Electro-Blot or photograph.

3. If using borosilicate glass plates, scan EtBr stained bands directly through glass without disassembly of gel plate sandwich.

3.12 Perpendicular DGGE Gel Casting

FIGURE 1

FIGURE 2

1.0mm AND THICKER

.75mm AND THINNER

PERPENDICULAR GEL CASTING

TO GRADIENT MAKER

20 GAUGE NEEDLE

SEAL WITH AGAROSE

HOLE THROUGH SPACER

GEL PLATE

CLAMPS

TO GRADIENT MAKER

20 GAUGE NEEDLE

SEAL WITH AGAROSE

GEL PLATE

CLAMPS

1. For perpendicular gel casting, locate the spacer which has a channel machined into it on one end which will be referred to as the “channel spacer”. The gel sandwich will be cast on its side, with the channel spacer on top and the channel further away from the single well comb (see Fig 3-11 (top) for casting gels 1.0mm and thicker, see Fig. 3-11 (bottom) for casting gels .75mm or thinner).

2. Follow the Gel Wrap instructions 1-5 (pages 36-37), making sure to use the channel spacer.

3. Insert single well comb into glass notch. A small amount of agarose is needed in two places once the gel sandwich is placed on its side, both places are located on the lower corner where the single well comb interfaces the spacer, and on the other side of the spacer where it interfaces the gel wrap.

Fig. 3-11 Top

Fig. 3-11 Bottom

4. Once the agarose has set, insert the gradient maker outow tube tipped with a 20ga. through

the Gel Wrap and into the “channel” (top), or space created between the horizontal spacer and the comb (bottom). You may use the same piece of Gel Wrap many times (20-30) for the perpendicular gels: do not use a piece of Gel Wrap Gasket for Vertical casting after you have poked a hole in it for perpendicular casting—it will leak.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.13 Perpendicular Gradient Formation

To determine the range of perpendicular gradient appropriate for your fragment analysis,

please read the enclosed paper by Myers, Shefeld and Cox, as well as the Methods and

Enzymology V. 212 paper by Abrams and Stanton. This gives you an excellent overview of the determination of melting behavior of your fragments.

1. The following is a typical protocol for casting a 40%-60% denaturing gradient gel. See section 4.2 for stock solutions. Refer to Section 3.5 Vertical Gradient Gel Casting for apparatus assembly. In an ice bucket, place two

50ml conical tubes labeled “A” and “B”. Add to tube “A”

 • 11.5ml of 40%/7.5%

 • 80 µI (10%) APS

 • 5 µl TEMED

2. Add to tube “B”:

  • 11.5ml of 60%/7.5%

  • 80 µI (10%) APS

  • 5 µI TEMED

3. Pour solution "B" into right side of gradient maker,(GM-40), and open interior valve to allow air bubble to escape. Let as much as 1 ml "B" solution BACKFLOW into left side of gradient maker. Decant the 1ml back into right side

with pasteur pipette. Remove any residual solution with absorbent paper.

4. Add solution “A” to left side of gradient maker.

5. Turn on magnetic stirrer.

6. Exit tube should be attached to near side of gel plate with tape.

7 Open inside (V-1) valve rst, then outside (V-2) valve to start ow.

8. Gel volume is 23ml, using the 0.75mm spacers. Allow 20-30 minutes for gel polymeraztion. If gel volume is not

enough to ll gel sandwich, use 0% to “top-off”.

9. After polymerization rinse gel plate assembly with D.I. water to remove excess acrylamide or denaturants from plate exterior.

3.13 Perpendicular Gradient Formation-continued

10. Remove comb and quickly transfer gel sandwich to cassette by removing all #2 clamps. LEAVE GEL WRAP IN PLACE. The Gel Wrap acts as a barrier and prevents perpendicular electrical elds from interfering with outside

lanes. Re-clamp to cassette using #1 clamps/4 per side. Dislodge the bottom section of the Gel Wrap from the

plates to allow buffer contact with the gel. (Fig 3-7, page 43)

11. Immerse cassette into pre-heated buffer tank, and attach recirculating tubes to each upper reservoir (Fig. 3-8, 3-9).

Rinse loading interface of gel with tank buffer to remove non-polymerized acrylamide or excess urea. Rinse each well with buffer and ll upper reservoir

12. Load samples.

13. Turn on power supply to 150V (40mA/one dual cassette) constant Volts for 5-7 hours.

14. When run is completed, turn off power supply, disconnect recirculating tubes and power leads, remove cassettes, remove glass sandwiches from cassettes.

15. Using a wedge plate separator,cat. # WPS-100, pry plates apart and immerse gel/plate in buffer tray. Stain with

EtBr (0.5g/ml) for 5-10 minutes. Lift oating gel on plate out of tray and rinse with DD H20. Flip over onto saran

wrap and peal off glass plate. View bands on transilluminator. Electro-Blot or photograph.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

3.14 Instructions for Use Buffer Siphon Pump

Dispose of solutions in accordance with the safety regulations of your institution.

1. Attach pump assembly to corner of tank as shown at left. (Fig 3-12)

2. Place the discharge tubing into a waste carboy located LOWER than the pump inlet.

3. Begin siphon with half strokes until liquid starts to ow.

Leave shaft, fully extended to allow siphon to operate.

4. To stop siphon, depress handle and remove pump from tank.

Fig. 3-12

3.15 Maximum Well/Comb Volumes

NOTE: To calculate sample well volume expressed in millimeters (mm) of height, divide maximum volume by tooth depth.

VGC xxxx -177 COMB OPTIONS SHORT WELLS

overall length = 13.1cm

Cat. # tooth depth

(mm)

VGC-0716-177 10 16 0.75 5.2 39

VGC-0720-177 10 20 0.75 4.2 31

VGC-0722-177 10 22 0.75 4.35 33

VGC-0729-177 10 29 0.75 2.9 22

VGC-0730-177 10 30 0.75 2.8 21

VGC-1016-177 10 16 1.0 5.2 52

VGC-1020-177 10 20 1.0 4.2 42

VGC-1022-177 10 22 1.0 4.35 43

VGC-1029-177 10 29 1.0 2.9 29

VGC-1030-177 10 30 1.0 2.8 28

VGC-1516-177 10 16 1.5 5.2 78

VGC-1520-177 10 20 1.5 4.2 63

VGC-1522-177 10 22 1.5 4.35 65

VGC-1529-177 10 29 1.5 2.9 44

VGC-1530-177 10 30 1.5 2.8 42

# of

teeth

thickness

of teeth

width of

teeth

maximum

well volume

(µl)

VGC xxxx COMB OPTIONS DEEPER WELLS

overall length = 13.1cm

Cat. # tooth depth

(mm)

VGC-0716 19.1 16 0.75 5.2 74

VGC-0720 17.7 20 0.75 4.2 56

VGC-0722 17.7 22 0.75 4.35 33

VGC-0729 17.7 29 0.75 2.9 38

VGC-0730 17.7 30 0.75 2.8 37

VGC-1016 19.1 16 1.0 5.2 98

VGC-1020 19.1 20 1.0 4.2 74

VGC-1022 17.7 22 1.0 4.35 76

VGC-1029 17.7 29 1.0 2.9 51

VGC-1030 17.7 30 1.0 2.8 49

VGC-1516 19.1 16 1.5 5.2 147

VGC-1520 17.7 20 1.5 4.2 111

VGC-1522 17.7 22 1.5 4.35 114

VGC-1529 17.7 29 1.5 2.9 77

VGC-1530 17.7 30 1.5 2.8 74

# of

teeth

thickness

of teeth

width of

teeth

maximum

well volume

(µl)

SECTION 4 Running Conditions

4.1 Recommended Power

1. 150V (40mA/one dual cassette) CV for 5-7 hours

4.2 Recommended Buffers

Warning: Donotusedeionizedwaterwithaspecicresistance>1megohm.

STOCK SOLUTIONS

Acrylamide Stock Solution 40% Acrylamide/Bis (37.5:1)

For 100ml use the following:

38.93 g of acrylamide

1.07 g of Bis-acrylamide Add dH2O to 100ml. Do not autoclave

50x TAE Gel Running Buffer

For 1 liter use the following: 242g Tris Base

57.1ml Glacial acetic acid 100ml .5 M EDTA, pH 8.0 Add dH2O to 1 liter

Denaturant Stock Solution (7.5% gel)

For 100ml use: 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%

40% Acrylamide/Bis (ml) 18.8 18.8 18.8 18.8 18.8 18.8 18.8 18.8 18.8 18.8

50x TAE Buffer (ml) 2 2 2 2 2 2 2 2 2 2 Formamide (ml) 4 8 12 16 20 24 28 32 36 40 Urea (g) 4.2 8.4 12.6 16.8 21 25.2 29.4 33.6 37.8 42

Add dH20 to 100ml

Store denaturant stock solutions at 4º C in amber bottles

Polymerization Catalysts:

1. TEMED

2. APS Stock Solution

For 50 ml of APS stock (10%) use 5 g of ammonium persulphate and water to 50ml. Store at 4ºC.

Neutral Loading Solution For preparation of 10ml:

20% Ficoll or sucrose, 2 g of Ficoll 400 or sucrose

10mM Tris-HCl, pH 7.8, 100ul of 1.0 M Tris-HCl, pH 7.8 1mM EDTA, 20 ul of 0.5 M EDTA, pH 8.0

0.1% dye (BPB), 10 mg of dye

Water to 10ml

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

SECTION 4 Running Conditions, con’t.

4.3 Recommended Running Conditions, TTGE

Sample PAA% % Denaturant

(UF/l)

K-ras exon 1 (5) 10 29 (7M UF/l) 1.7°C

PTEN (6) 10 29 (7M UF/l) 2ºC

p53 (7) 8 29 (7M UF/l) 3ºC

ß-thalassemia (7) 6 24 (6M UF/l) 2ºC

CF Exon 7 (11) 6 24 (6M UF/l) 2ºC

CF Exon 10, 11 (8) 6 24 (6M UF/l) 2ºC

mtDNA (10) 6 24 (6M UF/I) 1.2ºC

Nitrosospira-

Bacterial (9)

6 29 (7M UF/l) 1ºC

Temp Ramp

(°C/hour)

(63°-68°)

(46º-58º)

(56º-66º)

(50º-60º)

(53º-64º)

(40º-55º)

Time

(hours)

3 1x TAE 130

6 1x TAE 130

3.3

(mini-gel)

4.5 1.25x TAE 130

5 1.25x TAE 130

6-7 1.25x TAE 145

16 1.25x TAE 100

Buffer Volts

1.25x TAE 130

4.4 SSCP Running Conditions See references 13, 14.

4.5 References

1. K. Yoshino, K. Nichigaki, Y Husimi. (1991) Temperature sweep gel electrophoresis: a simple method to

detect point mutations. Nuceic Acids Res. 19:31-53

2. J. Bjorheim, S. Lystad, A. Lindblom, U. Kressner et al. (1998) Mutation analysis of KRAS exon 1 comparing three different techniques: temporal temperature gradient electrophoreis, constant denaturant

capillary electrophoresis and allele specic polymerase chain reaction. (1998) Mutat Res 403: 103-112

3. D. Riesner, K. Henco,& G. Steger (1991). Temperature-gradient gel electrophoresis: A method for

the analysis of conformational transitions and mutations in nucleic acids and proteins. In Advances in electrophoresis, Vol. 4 (Chrambach, A., Dunn, M.J. & Radola, B.J., Hrsg.). VCH Verlagsgesellschaft,

Weinheim, pp 169-250.

4. L. Lerman, K. Silverstien. Methods of Enzymol. (1987) 155, 482-501

5. S.G. Fischer and L.S. Lerman, (1983) PNAS 80:1579.

6. R.M. Myers, T. Maniatis, L.S. Lerman, (1987) Methods in Enzymology, 155: 501-529.

7. R.M. Meyers, V.C. Shefeld, D.R. Cox (1988) “Genome Analysis: A Practical Approach,” Ed. K Davies,

IRL Press, Oxford. PP. 95-13

8. V.C. Shefeld, D.R. Cox, L.S. Lerman and R.M. Meyers, (1989) PNAS, 86:232-236

9. Guldberg, P., Henriksen, K.F., and Guttler, F. (1993). “Molecular analysis of phenylketonia in Denmark:

99% of the mutations detected by denaturing gradient gel electrophoresis.” Genomics 17:141-146

10. Moyret, C., Thellet, C., Puig, P.L., Moles, J-P., Thomas, G. and Hamelin, R. (1994). “Relative efciency of

denaturing gradient gel electrophoresis and single-strand conformation polymorphisms in the detection of mutations in exons 5 to 8 of the p53 gene.” Oncogene 9:1739-1743

11. Shefeld, V.C., Beck, J.S., Kwitek, A.E., Sandstrom, D.W., and Stone, E.M. (1993). “The sensitivity of

single-strand conformation polymorphism analysis for the detection of single bas substitutions.” Genomics 16: 325-332.

12. Abrams, E.S. Stanton, V.P. Jr., (1992) “Use of Denaturing Gradient Gel Electrophoresis to Study Conformational Transitions in Nucleic Acids” Methods in Enzymology 212:71-104.

13. Nataraj, A., Olivos-Glander, I., Highsmith Jr., W.E. (1999) “Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection.” Electrophoresis 20:1177-1185

14. Glavac, D., Dean, M., (1993) Optimization of the Single-Strand Conformation Polymorphism (SSCP) Technique for Detection of Point Mutations” Human Mutation 2:404-414

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

SECTION 5

MaintenanceofEquipment

5.1 Care and Handling

The plastic components of the DGGE units are fabricated from acrylic and polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and reliable performance.

After each use, rinse buffer chamber, gel tray and combs with de-ionized water. Wipe dry with a soft cloth or paper towel, or allow to air dry. Whenever necessary, all components may be washed gently with water and a non-abrasive detergent, and rinsed and dried as above. Never use abrasive cleaners, glass cleaning sprays or scouring pads to clean the components, as these will damage the unit and components.

Additional precautions:

• Do not autoclave or dry-heat sterilize the apparatus or components.

• Do not expose the apparatus or components to phenol, acetone, benzene, halogenated hydrocarbon

solvents or alcohols.

• Avoid prolonged exposure of the apparatus or components to UV light.

• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended periods at 37°C. A

brief rinse with DEPC-water is sufcient after a thorough wash.

5.2 Maintenance & Calibration

The following inspection and maintenance procedures will help maintain the safety and reliable performance of the

Mini-Horizontal systems. Replacement parts can be ordered by calling 858-755-4959 or by contacting your local

distributor.

• Banana plugs and power cords should be inspected regularly. If the banana plugs become loose or do not feel friction tight replace the plugs or power cords.

• Should power cord assemblies (connectors, wire or shrouds) show any signs of wear or damage (e.g. cracks, nicks, abrasions, or melted insulation), replace them immediately.

• The platinum wire is secured to the banana jack by compression between a stainless washer and the jack nut. The nut/washer interface should be tight and free of corrosion.

5.2a Heater

The heater should be kept clean. If deposits build up on the heater, they may be removed by scrubbing with a nonmetallic (plastic) abrasive pad. Do not use steel wool.

5.2b Pump Motor

The pump bearings are permanently lubricated with high-temperature silicone grease and do not require additional lubrication. Should the bearings become noisy, replacement of the entire pump motor is recommended. This will

reduce repair labor costs and retain uid pumping reliability.

A replacement pump and motor mounting kit is available (see Section 10 - Replacement Parts).

5.2c Calibration

At times there may be a minor temperature difference between the Controller’s displayed temperature and the actual

temperature as determined by a certied temperature measurement device. There may also be situations where

you want the displayed temperature to match a particular value to have standardization between different laboratory instruments. These adjustments can be performed via the Controller’s password-protected calibration display(s).

5.2 Maintenance & Calibration- continued

To access the calibration display(s), rotate the Select/Set Knob until the Instrument Identication display appears.

This is the last accessible screen as you rotate the Select/Set Knob counter- clockwise.

With the Instrument ID screen displayed, press and hold the Timer Button while also pressing the Select/Set Knob. A password box will appear on the Instrument ID screen.

The Calibration access password is TUSER. It is case sensitive and entered by rotating the Select/Set Knob until the

desired character appears and then pressing the Select/Set Knob. The cursor will automatically advance to the next

eld in the password entry display. Once the nal character is entered, rotate the knob and the following Calibration

screen(s) will be available.

On Digital Controllers, only the Calibration User - Internal Probe Calibration will appear. On Programmable Controllers, the Calibration User – External Probe Calibration screen will also appear if an external probe is connected to the Controller.

To perform a calibration, go to the appropriate Calibration User screen and press the Select/Set Knob. Rotate the

Select/Set Knob until the offset value equals the difference between the probe temperature reading and the reference temperature probe. Press the Select/Set Knob to accept the offset value.

NOTE: Allow the temperature reading at the probe to stabilize before making adjustments.

NOTE: The Calibration User display(s) remains available only while Controller power is On. If the Controller in placed in Standby or main power is disrupted, the display(s) will have to be re-enabled by entering the Calibration access password.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

SECTION6 HEATER/STIRRER–Troubleshooting

6.1UnitWillNotOperate(noheat,coolingorpumping)

• Check that the power is plugged into an operating electrical outlet.

• Check that the power is plugged into an operating electrical outlet

6.2NoPumping

• Check that the power is plugged into an operating electrical outlet

6.3SloworInsufcientPumping

• Check that the power is plugged into an operating electrical outlet.

• Check that the power is plugged into an operating electrical outlet

6.4NoHeating

• Check that the power is plugged into an operating electrical outlet.

• Check that the power is plugged into an operating electrical outlet

 6.5InsufcientHeating

• Check that the power is plugged into an operating electrical outlet.

• Check that the power is plugged into an operating electrical outlet

 6.6TriacFailure

• Triac is Open fault message appears on the display, indicating that the heater triac has failed or the line

supply voltage has a source of extreme interference

SECTION6 HEATER/STIRRER–Troubleshooting - continued

6.7ExternalProbeFailure

• External Probe Open or External Probe Short fault message appears on the display, indicating that the external probe has failed or there is a problem with the circuitry reading the probe signal. Replace the

external probe; if the problem persists, an internal PCB needs replacement.

6.8RecallingthePower-UpLanguageSelectionMenu

The rst time the Controller is powered up, a language selection menu is displayed which allows the user

to select the language used for operational displays and programming. This is a one-time display; the next time the Controller is powered up, either Standby or the main operational display will appear once the initial self-test procedure has been completed.

You may change the language used for operational and programming displays in either of two ways. One is to change the language using the Preferences sub-menu. The other is to recall the initial power-up language selection display. This is performed as follows.

1. Press the Power Button to turn Controller power off (Standby displayed).

2. Place the circuit breaker/power switch on the rear panel in the Off position (display blank).

3. Press and hold ESC Button.

4. Place the circuit breaker/power switch in the On position; continue holding ESC Button.

5. Release the ESC Button when the language selection menu is displayed.

6. Rotate the Select/Set Knob until the desired language is highlighted.

7. Press the Select/Set Knob.

The Controller will complete the start-up sequence and display “Standby” on the LCD. All operating and programming screens will now be displayed in the selected language.

DONOTUSEthefollowinguids:

1. Automotive antifreeze with additives**

2. Hard tap water**

3.Deionizedwaterwithaspecicresistance>1megohm

4. Any ammable uids

5. Concentrations of acids or bases

6. Solutions with halides: chlorides, uorides, bromides, iodides or sulfur

7. Bleach (Sodium Hypochlorite)

8. Solutions with chromates or chromium salts

** At temperatures above 40°C, additives or mineral deposits can adhere to the heater. If deposits are

allowed to build up, the heater may overheat and fail. Higher temperatures and higher concentrations of additives will hasten deposit build up.

Warning: Do not use a ammable liquid as a bath medium as a re hazard may result. Be aware of the ash point temperatures for the uids used.

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

SECTION 7 DTSK-2401 & DTSK-2001 ACCESSORIES

SPACER SETS- Vertical DGGE SPACER SETS- Perpendicular DGGE

(Heat resistant polycarbonate) (Spacer sets made from heat resistant polycarbonate: 1 standard spacer & 1

Cat.# Spacer Dimensions Cat.# Spacer Dimensions

VGS-0420R-177 0.4mm x 22cm VGS7520RC-177 0.75mm x 22cm VGS-0520R-177 0.5mm x 22cm VGS1020RC-177 1.0mm x 22cm VGS-7520R-177 0.75mm x 22cm VGS1520RC-177 1.5mm x 22cm VGS-1020R-177 1.0mm x 22cm VGS2020RC-177 2.0mm x 22cm VGS-1520R-177 1.5mm x 22cm VGS-2020R-177 2.0mm x 22cm

Gel Wrap Gasket Combs for Perpendicular DGGE

Cat.# Spacer Thickness x Plate Height Cat.# Comb Description

VGE-0520-177 0.5mm x 22cm VGC-7501-177 0.75mm x 1 well VGE-7520-177 0.75mm x 22cm VGC-1001-177 1.0mm x 1 well VGE-1020-177 1.0mm x 22cm VGC-1501-177 1.5mm x 1 well VGE-1520-177 1.5mm x 22cm VGC-2001-177 2.0mm x 1 well VGE-2020-177 2.0mm x 22cm

Combs for DGGE - short wells Combs for DGGE - long wells

Cat.# Comb Description Cat.# Comb Description

VGC-0716-177 0.75mm x 16 well VGC-0716 0.75mm x 16 well VGC-0720-177 0.75mm x 20 well VGC-0720 0.75mm x 20 well VGC-0722-177 0.75mm x 22 well VGC-0722 0.75mm x 22 well VGC-0729-177 0.75mm x 29 well VGC-0729 0.75mm x 29cm VGC-1016-177 1.0mm x 16 well VGC-1016 1.0mm x 16 well VGC-1020-177 1.0mm x 20 well VGC-1020 1.0mm x 20 well VGC-1022-177 1.0mm x 22 well VGC-1022 1.0mm x 22 well VGC-1029-177 1.0mm x 29 well VGC-1029 1.0mm x 29cm VGC-1516-177 1.5mm x 16 well VGC-1516 1.5mm x 16 well VGC-1520-177 1.5mm x 20 well VGC-1520 1.5mm x 20 well VGC-1522-177 1.5mm x 22 well VGC-1522 1.5mm x 22 well VGC-1529-177 1.5mm x 29 well VGC-1529 1.5mm x 29cm

spacer with injection port.)

Bulk Gel Wrap Gasket Gel Cassettes

Cat.# Gel Thickness Cat.# Item

VGE-05XX 0.5mm thick-yellow DGC-177-201 Additional single gel cassette VGE-75XX 0.75mm thick-yellow DDGC-177-201 Additional dual gel cassette VGE-10XX 1.0mm thick-red

VGE-15XX 1.5mm thick-blue Buffer Siphon Pump VGE-20XX 2.0mm thick-purple Cat.# Item

VGE-30XX 3.0mm thick-white BSP-1000 Buffer Sipon Pump

DGGE Glass Plate Sets, notched

(1/8” back plate with rounded corners) White Clamps

Cat.# Glass Plate Dimensions Cat.# Item

NGP-17720-NR 17.7cm (w) x 22cm (h) GPC-0001-177 White clamp for holding glass plate

NGP-17720-B 17.7cm (w) x 22cm (h) borosilicate sandwich to upper reservoir. (Jaws slightly open in resting position)

GPC-0002-177 White clamp for gel casting (Jaws closed in resting position)

Power Supplies

Cat.# Description

EPS-300-II Mini-Power Supply, 110V/60Hz EPS-300-IIV Mini-Power Supply, 220V/60Hz

www.cbsscientic.com

Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

Telephone: Local or International 858-755-4959 Toll Free: 858-755-0733

Fax: 858-755-0733

Check out our new and improved online ordering!

www.cbsscientiﬁc.com

E-mail address: sales@cbssci.com

Mailing address: C.B.S. Scientiﬁc Company P.O. Box 856 Del Mar, CA 92014

Shipping address: C.B.S. Scientiﬁc Company 10805 Vista Sorrento Pkwy Suite 100 San Diego, CA 92121

Credit Card Options: Visa/Mastercard Discover/American Express

C.B.S. Scientific DTSK-2401-220 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual