C.B.S. Scientific DCX-900C User Manual

Large Format Cooled Gel System

Vertical Slab Gel System

DCX-900C

INSTRUCTION MANUAL

TABLE OF CONTENTS

Important User Information . . . . . . . . . . . . . . . . . . . . . . . .3-4

Section 1: General Information

1.1 Introduction and Features . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Specications and Constructions

1.3 Comb Specications for Hand Cast Gels . . . . . . . . . . . . . . . . 6

1.4 Safety

Section 2: Description of Parts

2.1 Unpacking and Components . . . . . . . . . . . . . . . . . . . . . 7

Section 3: Instructions for Slab Gel Electrophoresis

3.1 Preparing Electrophoresis Unit

3.2 Running the Gel

3.3 Removing the Gel

3.4 Gel Casting Using Gel Wrap Gasket

Section 4: Running Conditions

4.1 Recommended Power for Slab Gels

4.2 References . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Section 5: Maintenance of Equipment

5.1 Care and Handling . . . . . . . . . . . . . . . . . . . . . . . . 15

5.2 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

. . . . . . . . . . . . . . . . . . . . . . . . 10

. . . . . . . . . . . . . . . . . . . . . . . 11

. . . . . . . . . . . . . . . . . . . . 5

. . . . . . . . . . . . . . . . . . 8-9

. . . . . . . . . . . . . . . . .12-13

. . . . . . . . . . . . . . . . . 14

Section 6: Ordering Information

6.1 Ordering Information for Large Format Cooled Vertical System . . . . . . . 16

6.2 Ordering Information for Related Products . . . . . . . . . . . . . . . 16

Section 9: Contact Information . . . . . . . . . . . . . . . . . . . . . 20

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DCX-900C Large Format Instructions 9-30-13

ENGLISH

IMPORTANT USER INFORMATION

This Instruction Manual will explain how to use this product safely and effectively.
Please read and carefully follow the instruction manual in its entirety.

The triangle/exclamation mark symbol alerts the user of the product to important opera­tional, maintenance, and/or warranty requirements.

The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous
electrical exposure.

Failure to adhere to the instructions could result in personal and/or laboratory hazards,
as well as invalidate any warranty. Always turn off the DC power source prior to discon­necting power cords from the product. Disconnect power cords from the power source

rst and then from the product. For maximum safety, always operate this system in an
isolated, low trafc area, not accessible to unauthorized personnel. Never operate dam-

aged or leaking equipment.

WARRANTY AND LIABILITY

This product was produced utilizing the highest practical standards of materials, work-

manship, and design. C.B.S. Scientic warrants that the product has been tested and
will meet or exceed published specications. This warranty is valid only if the product

has been operated and maintained according to the instructions provided.

C.B.S. Scientic warrants this product to be free from defects in materials and work­manship under normal service for one year from date of shipment. If the product

proves defective during this period, C.B.S. Scientic will repair or replace it at our

option, free of charge, if returned to us postage prepaid. This warranty does not
cover: damage in transit, damage caused by carelessness, misuse or neglect, normal
wear through frequent use, damage caused by solvent corrosion, damage caused by
improper handling or user alteration, nor unsatisfactory performance as a result of con-

ditions beyond our control. C.B.S. Scientic shall in no event be liable for incidental nor
consequential damages, including without limitation, lost prots, loss of income, loss

of business opportunities, loss of use and other related damages, however caused, nor
any damage arising from the incorrect use of the product.

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FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURS

Le présent manuel d’utilisation explique la manière de se servir efcacement du produit en conditions

de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes

et ses instructions.

Le triangle avec point d’exclamation est un symbole destiné à avertir l’utilisateur du produit

de l’importance de certaines exigences relatives au fonctionnement, à l’entretien et/ou à
la garantie.

Le triangle avec èche en zigzag est un symbole destiné à avertir l’utilisateur du produit de la
possibilité d’exposition à des décharges avec danger de secousses électriques.

Tout manquement à l’observation des consignes et des instructions peut exposer les personnes
et les biens à des dommages corporels et/ou matériels et peut annuler toute garantie. Il faut toujours
interrompre l’alimentation de courant continu avant de déconnecter les cordons d’alimentation du
produit. Déconnecter d’abord les cordons d’alimentation branchés sur la source de tension (alimentation
de secteur) puis ceux branchés sur le produit. Pour une sécurité maximum, il faut toujours faire
fonctionner ce système dans un lieu isolé, peu fréquenté, où le personnel non autorisé n’a pas accès. Ne
jamais faire fonctionner un matériel endommagé ou affecté par des fuites.

GARANTIE ET RESPONSABILITÉ

Le produit a été fabriqué conformément aux normes applicables les plus exigentes en matière de
matériaux, de main d’oeuvre, de conception et d’ingéniérie. C.B.S. Scientic garantit que le produit a subi
des essais et que ses performances rempliront les conditions des spécications publiées ou leur seront
même supérieures. La présente garantie n’est valide que si le produit a fonctionné et a été entretenu
conformément aux consignes et instructions fournies.

C.B.S. Scientic garantit que le produit sera dépourvu de vices de matériaux et de main d’oeuvre, en
conditions de service normales, pendant un an à compter de la date d’expédition. Au cas où le produit
s’avérerait défectueux pendant cette période de garantie, C.B.S. Scientic réparera ou remplacera le
produit, à sa discrétion et gratuitement, si le produit lui est retourné port payé d’avance. La garantie
ne couvre pas les dommages de transport; les dommages causés par l’imprudence, le manque de
soins, l’abus ou la négligence; l’usure normale résultant d’une utilisation fréquente; les dommages
causés par la corrosion des solvants; et les dommages causés par la manipulation inadéquate ou
des changements apportés par l’utilisateur. La garantie ne couvre pas non plus les performances non
satisfaisantes résultant de conditions hors du contrôle de C.B.S. Scientic. C.B.S. Scientic ne pourra
en aucun cas être tenue responsable de dommages indirects, y compris, de manière non limitative, la
perte de bénéces, le manque à gagner, la perte d’occasions d’affaires, l’impossibilité d’usage ou tous
autres dommages associés, quelle qu’en soit la cause, ni de dommages résultant de l’usage incorrect

du produit.

ESPAÑOL INFORMACIÓN IMPORTANTE PARA EL USUARIO

El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase
leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.

El símbolo del triángulo con exclamación llama la atención del usuario a requisitos
importantes para el uso y mantenimiento del producto, así como para la validez de
la garantía.

El símbolo del triángulo con rayo llama la atención del usuario a la posibilidad de riesgos
eléctricos.

El incumplimiento de las instrucciones aquí señaladas podría dar lugar a riesgos a la persona, al
laboratorio o a ambos y podría anular toda garantía. Siempre apague la fuente de corriente continua
antes de desenchufar los cables eléctricos del producto. Primero desconecte los cables de la fuente de
energía y después del producto. Para mayor seguridad, siempre use este sistema en un área aislada,
de poco movimiento de personas e inaccesible a personal no autorizado. Jamás use equipo que
presenta algún daño o fuga.

GARANTÍA Y RESPONSABILIDAD

Este producto fue fabricado de acuerdo con las normas más estrictas que sean factibles en cuanto a
materiales, mano de obra y diseño. C.B.S Scientic garantiza que se sometió el producto a pruebas
y que cumplirá o excederá las especicaciones publicadas. Esta garantía será válida únicamente si
se usa y se da servicio de mantenimiento al producto de acuerdo con las instrucciones señaladas.

C.B.S. Scientic garantiza que este producto se encontrará libre de defectos de materiales y mano de
obra por un período de servicio normal de un año a partir de la fecha de embarque. Si el producto resulta
defectuoso durante este período, C.B.S. Scientic lo reparará o lo repondrá, a criterio de C.B.S., libre
de cargos, si se devuelve el producto a C.B.S. porte pagado. Esta garantía no cubre daños sufridos en
tránsito, daños provocados por descuido, mal uso o negligencia, desgaste normal como consecuencia
del uso excesivo, daños atribuibles a corrosión provocada por solventes, daños causados por el uso
indebido o alteraciones realizadas por el usuario ni rendimiento insatisfactorio atribuible a circunstancias
fuera del control de C.B.S. Scientic. C.B.S. Scientic en ningún caso asumirá responsabilidad por
daños incidentales o subsecuentes, incluyendo, en forma no limitativa, la pérdida de utilidades, de
ingresos, de oportunidades comerciales o del uso del producto y otros daños anes, fuere cual fuere su
origen, ni por daños derivados del uso incorrecto del producto.

DEUTSCH WICHTIGE INFORMATION FÜR DEN BENUTZER

Diese Bedienungsanleitung beschreibt wie man dieses Produkt sicher und wirksam benutzt. Bitte lesen
und befolgen Sie alle Anweisungen in dieser Anleitung.

Das Dreieck mit Ausrufezeichen weist den Benutzer des Produktes darauf hin, daß
wichtige Bedienungs-, Wartungs- und/oder Garantievorschriften zu beachten sind.

Das Dreieck mit Zickzackblitz warnt den Benutzer des Produktes vor möglichen
Gefahren durch elektrische Spannungen.

Nichtbeachtung dieser Anweisungen kann zu persönlichen und/oder labortechnischen Schäden
führen und gleichzeitig alle Garantien als nichtig erklären. Die DC Stromzufuhr muß immer, vor dem
Entfernen der Stromkabel vom Produkt, abgeschaltet werden. Die Stromzufuhrkabel müssen zuerst von
der Steckdose und erst dann vom Produkt entfernt werden. Um höchste Sicherheit zu gewährleisten
sollte dieses System in einem abgesonderten und besonders ruhigen Bereich eingesetzt werden und
vor Unbefugten sicher sein.

GARANTIE UND HAFTUNG

Dieses Produkt wurde unter Anwendung von Produkten mit höchster Qualität und aus Materialien mit
bester Verarbeitung und modernstem Design hergestellt. C.B.S Scientic garantiert, daß das Produkt
getestet wurde und alle publizierten Spezikationen übertrifft. Diese Garantie ist jedoch nur gültig, wenn
das Produkt nach der beigefügten Bedienungsanleitung bedient und gewartet wurde.

C.B.S. Scientic garantiert, daß dieses Produkt bei normaler Bedienung aus fehlerfreiem Material
besteht und fehlerfrei in der Ausführung ist. Diese Garantie gilt für ein Jahr ab Lieferdatum. Sollte
das Produkt in diesem Zeiraum fehlerhaft werden, bietet C.B.S. Scientic eine kostenlose Reparatur
bzw. kostenlosen Ersatz, einschließlich freiem Rückporto. Diese Garantie schließt folgendes aus:
Transportschaden, Schaden durch Nachlässigkeit, Mißbrauch oder Vernachlässigung, normale
Abnützung durch regelmäßigen Gebrauch, Schaden durch Säureangriff, Schaden durch falsche
Handhabung, Veränderung des Produktes durch den Benutzer, oder unzureichende Leistungen die
sich nicht im Verantwortungsbereich von C.B.S. Scientic benden. C.B.S. Scientic kommt unter
keinen Umständen für folgende Schäden auf: Sachschadensverlust, Einkommensverlust, Verlust
von Geschäftsmöglichkeiten, Verlust der Anwendung und andere damit verbundene Schäden die auf
irgend eine Art und Weise entstanden sind, oder Schäden die aus falscher Anwendung des Produktes

entstanden sind.

ITALIANO INFORMAZIONI IMPORTANTI PER L’UTENTE

Questo manuale spiega come utilizzare questo prodotto in maniera sicura ed efciente. Si pregai di
leggere e seguire con cautela le istruzioni di ogni parte di questo manuale.

Il triangolo contenete il simbolo di un punto esclamativo avverte l’utente di importanti
requisiti relativi al funzionamento, manutenzione e/o garanzia del prodotto.

Il triangolo contenete il simbolo di un lampo avverte l’utente del prodotto della possibilità
di pericoli dovuti a corrente elettrica.

La mancata osservanza delle istruzioni può essere causa di pericolo alla propria persona ed
al laboratorio, oltre a poter annullare la garanzia. Prima di distaccare il cordone d’alimentazione dal
prodotto, spegnere sempre la sorgente di corrente continua. Distaccare i cordoni d’alimentazione prima
dal lato della sorgente di tensione e poi dal lato del prodotto. Per maggior sicurezza, mettere sempre
in funzione il prodotto in un’area isolata con poco trafco che non sia accessibile al personale non
autorizzato. Non mettere mai in funzione un’apparecchiatura che sia danneggiata o abbia perdite.

GARANZIA E RESPONSABILITÀ

Questo prodotto è stato fabbricato seguendo gli standard più elevati per i materiali, la manodopera e la
progettazione. La C.B.S. Scientic garantisce il prodotto è stato sottoposto a prova e raggiunge o supera
i valori pubblicati per i dati tecnici. Questa garanzia è valida solo se il prodotto è messo in esercizio e
soggetto a manutenzione secondo le istruzioni fornite.

La C.B.S. Scientic garantisce che questo prodotto è libero di difetti di materiali e manodopera, in normali
condizioni d’esercizio, per la durata di un anno dalla data di spedizione. Se, in questo periodo, il prodotto
si dimostrerà difettoso, la C.B.S. Scientic, a suo giudizio, lo riparerà o sostituirà. Questa garanzia non
copre danni in transito, danni causati da negligenza, uso improprio, trascuratezza, normale consumo
derivante da uso frequente, o danni causati da solventi corrosivi, danni causati da maltrattamento o da
modiche apportate dall’utente e non copre prestazioni insoddisfacenti che siano il risultato di condizioni
al di fuori del controllo del fabbricante. La C.B.S. Scientic non sarà in ogni caso responsabile per danni
incidentali o consequenziali, incluso, senza limitazioni, perdite di protto, perdita di entrate, perdita di
opportunità d’affari e altri danni relativi, comunque causati, e per danni risultati da uso incorretto del

prodotto.

4

DCX-900C Large Format Instructions 9-30-13

SECTION 1
General Information

1.1 Introduction and Features

C.B.S. Scientic offers the Large Format Cooled Vertical is a temperature controlled

large capacity system for running SDS PAGE and 2-D analysis. The increased gel sizze
of 20cm(w) x 22cm(l) allows a larger sample volumes, increased number of wells and
longer migration distance.

Table 1: Features of DCX-900C

Model # Single or

Dual

DCX-900C Dual 20cm x 22cm Double-sided cooling

Plate Dimensions
(w x h)

Cooling

labyrinths

1.2 Specications and Constructions

Constructions:

Buffer chamber, safety cover, core Polycarbonate/Polysulphone

Electrodes Pure Platinum wire .010” diameter
Power cords Silicone rated 7500VDC,
200mA, 65°C
Combs Teon
Glass plates Soda-lime oat glass & Borosilicate
Spacers PVC
Clamps Polypropylene, stainless steel

Safety Certication EN61010-1-1993 (IEC1010-1)

Table 2: Specications

Model # DCX-900C

Shipping Weight 10 lbs

Overall Size W x D x H (cm)

Recommended buffer volumes:
Cathode reservoir
Anode reservoir

Slab gel run time

24.2 x 12.4 x 27

1 Liter
800mls (minimum) / 3.2 Liters (maximum)

30-90 minutes

Blot run time

Handcast glass plate size (W x H)

Voltage limit

60-90 minutes

20cm x 22

300 V

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1.3 Comb Specications for Hand Cast Gels

COMB OPTIONS

material: PTFE, overall length: 15.87cm

Cat. # # of

Teeth

SG20-0708 8 19.05 0.75 17.7 95
SG20-0712 12 19.05 0.75 11 58
SG20-0717* 17 19.05 0.75 6.63 35
SG20-0720 20 19.05 0.75 5.66 30
SG20-0728 28 17.45 0.75 4 22
SG20-0732 32 17.45 0.75 3.4 18
SG20-0735* 35 17.45 0.75 2.9 14
SG20-1008 8 19.05 1.0 17.7 126
SG20-1012 12 19.05 1.0 11 78
SG20-1017* 17 19.05 1.0 6.63 50
SG20-1020 20 19.05 1.0 5.66 40
SG20-1028 28 17.45 1.0 4 29
SG20-1032 32 17.45 1.0 3.4 24
SG20-1035* 35 17.45 1.0 2.9 19
SG20-1508 8 19.05 1.5 17.7 190
SG20-1512 12 19.05 1.5 11 117
SG20-1517* 17 19.05 1.5 6.63 71
SG20-1520 20 19.05 1.5
SG20-1528 28 17.45 1.5 4 43
SG20-1532 32 17.45 1.5 3.4 36
SG20-1535* 35 17.45 1.5 2.9 29
SG20-2008 8 19.05 2.0 17.7 252
SG20-2012 12 19.05 2.0 11 156
SG20-2017* 17 19.05 2.0 6.63 94
SG20-2020 20 19.05 2.0 5.66 80
SG20-2028 28 17.45 2.0 4 57
SG20-2032 32 17.45 2.0 3.4 48
SG20-2035* 35 17.45 2.0 2.9 38

Tooth

Depth

(mm)

Thickness

of Teeth

(mm)

Width of

Teeth
(mm)

5.66 60

Recommended

Max. Sample

Vol./Well

(µl)*

*

Multi-channel pipettor
compatible

*

Maximum loading

volume is calculated by
taking 75% of the total
tooth volume.

*Comb conguration is

one large lane and one
marker lane

1.4 Safety

Power to the Large Format Cooled Vertical Electrophoresis System is to be supplied

by an external DC voltage power supply that must be ground isolated so that the DC

voltage output oats with respect to ground. For any power supply used, the maxi­mum specied operating parameters for the units are:

Maximum Limits

300 VDC
30 watts power
190mA current

60°C ambient temperature

Current to the unit, provided from the external power supply, must enter the unit
through the lid assembly, providing a safety interlock to the user. Current to the unit
is broken when the lid is removed. Do not attempt to use the unit without the

safety lid, and always turn the power supply off before removing the lid, or when

working with the unit in any way. Follow safety precautions specied by the

power supply manufacuturer.

6

DCX-900C Large Format Instructions 9-30-13

SECTION 2

Description of Parts

2.1 Unpacking and Components

Please verify that your unit comes complete with the following components:

Large Format Cooled Vertical Electrophoresis System:

• Lower Reservoir with double-sided cooling labyrinth, tubing adapters
and stirring corral

• Safety cover

• Power leads (must be attached before using. See section 3.1.1)

• Core

• 2 sets of Gel Wrap Gasket (not shown)

• 2 sets of Gel Wrap Glass Plates (not shown)

• 2 sets of Gel Wrap Spacers (not shown)

• 2 Combs (not shown)

• 12 White clamps (not shown)

• Conversion plate for running single gels (not shown)

Safety Cover

Lower reservoir with side cooling labyrinths

7

Core

Tubing adaptors

Power leads

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SECTION 3

Instructions for Slab Gel Electrophoresis

See Section 4 for casting gels using the Gel Wrap method. You may also use
the 4-place Multi-Caster DCX9-MC-4.

3.1 Preparing the Electrophoresis Unit

1. Power leads are shipped separately and

must be attached before using your
Large Format Cooled Vertical. Thread

the power lead ends into the lid recep­tacles and rotate in clockwise direction to
hand tighten.

2. Place unit in authorized work area. Re­move safety cover from the assembled
unit by simultaneously pressing down on
white push pins while lifting up on blue

safety cover as shown in gure 1.

Do not remove safety cover by pulling

up on leads!

1

3. Remove white core from lower reservoir

by grasping core with one hand and lifting

directly up as shown in gure 2.

4. Open doors on the core assembly by pull­ing up on the white latches.

8

2

DCX-900C Large Format Instructions 9-30-13

5. Slide gel plate set(s) into the core
assembly with the notched plate

facing in towards the upper buffer
reservoir as shown in gure 4.

6. If running one gel, slide white plastic
conversion plate into the side without
the gel.

3

7. Close doors and relatch by pressing
down on the white latches so that
the assembly looks like that shown in

gure 6.

4

5

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3.2 Running the Gel

1. Place stirring bar into bottom of reservoir in
stirring corral (as shown in gure 6). Place
core assembly into lower reservoir. The
anode (red) and cathode (black) electrodes
are color-coded on both the core/cassette
assembly and lower reservoir. Ensure the
red dot on the cassette assembly is on the
same side as the red receptacle on the lower
reservoir. Fill core upper reservoir with freshly

prepared buffer (~ 190mls).

If any buffer is spilled into banana jack

receptacles (outlined in yellow boxes in

gure 6) in lower reservoir, dry completely

using compressed air! Failure to do this
will result in accelerated banana jack cor­rosion.

2. If using cooling capability of DCX-900C at-
tach external cooler to tubing adapters and
begin circulating coolant through base.

stirring bar

6

3. Pour only enough freshly prepared buffer into
lower chamber so that the nal buffer level

is just below bottom of sample wells. Using

a pipette or syringe, thoroughly ush out the

wells in the glass plate sandwich with buffer.
Load samples. If outer lanes do not con­tain sample, it is recommended that you run

standards and/or ll outer lanes with loading

buffer to reduce smiling and wrap-around ef­fects.

4. Attach safety cover and turn on magnetic
stirrer. The closed unit ready for power is

shown in gure 7.

5. Connect the leads to the power supply,
matching the color-coded red to red and
black to black. See Section 4.1 for recom-
mended power conditions. Begin separa­tion by electrophoresis.

10

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DCX-900C Large Format Instructions 9-30-13

3.3 Removing the Gel

1. Turn the power supply off and disconnect
the leads from the power supply. Remove
the safety cover from the unit, by placing

thumbs on white posts next to red & black

connectors, then pushing down while pulling

up with ngers under lid as shown in gure

8.
Do not remove safety cover by pulling up
on leads!

2. Pull up on gel door latches, and open gel
door. Remove gel sandwich from Cassette

Assembly. Stain and x according to your

preferred method.

8

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3.4 Gel Casting Using Gel Wrap™ Gasket Casting Method

1. Place all components in an authorized work area. You will need: glass plate set, Gel
Wrap™ Gasket, spacer set, comb, 3 GPC-0002 clamps, and polyacrylamide solu­tion. Prepare and clean glass plates by hand washing both plates with a high quality
lab detergent followed by a complete rinsing with dH2O. Air-dry or use a lint-free

tissue. Spray/wipe the chosen inner surfaces of the plate set with 95% ethanol and

dry with lint-free tissue.

2. Hold the rectangular back plate with
the rounded bottom corners and start
applying the gasket around one side of

the glass plate. Note: one side of the “U”
shaped gasket is at, and the other side

has tubing that will act as a seal around
the spacers.

3. When applying the gasket over the

rounded corners of the back glass plate,
make sure the notches on the gasket
align with the rounded corners of the
glass plate. Once the gasket is pushed
over the bottom edge and corners, work
it down the remaining side.

4. Place the gasketed plate on the lab
bench with the tubing side up, and
extend the bottom of the plate over the
edge of the bench, approximately ¾ of
an inch. Place the spacers along side
the inside edges of the gasket. Be sure
the rounded corner end of each spacer
is facing the outside bottom of the plate,
following the radius of the glass.

12

DCX-900C Large Format Instructions 9-30-13

5. Place the notched plate on top of the bottom
assembly, starting from the bottom edge and

gently easing the plate down. Verify the gasket

is smooth around the edges and then clamp
along the bottom.

6. Lift the assembly and stand it on the base

of the clamps. For leveling, push glass plate
assembly down until it stops against clamp
body. Clamp the sides of the assembly with
additional casting clamps on either side. As
each clamp is attached, be sure the gasket is
aligned between the plates forming a seal.

7. Apply PAGE or agarose solution to gel plate
sandwich using a syringe or pipette. If using
a stacking gel, pour desired height of running
gel, then overlay a small amount of dH2O

or 0.1% SDS solution to top of gel. After

polymerization, rinse with buffer, add stacking
gel solution and insert comb. For regular, unit
percentage gels, add polyacrylamide solution
to correct height, and insert comb. Allow gel
to set, usually 20 minutes. Extra gel solution
in pipette or syringe can be monitored to test
polymerization of gel mix.

8. Disassembly. Hold the clamped plate as­sembly with one hand. Remove the gasket by
starting at one of the top ends and pulling up
and out on the gasket until it releases from the
plate, up to the bottom of each of the white
clamps. When each clamp is reached DO

NOT remove it, instead feed the gasket down

through the clamp body and repeat pulling up
and out. Continue feeding until the gasket is
fully detached. If gel, is not to be used imme­diately, wrap entire plate sandwich with plastic
wrap tightly to seal and store at 4°C for up to
a month.

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SECTION 4
Running Conditions

4.1 Recommended Power for Slab Gels:

Precise electrophoresis conditions will vary according to the number and type of
gels used, buffer conditions employed, power input, and the general goal of the
experiment. Refer to reference section 4.2 for in depth discussions on practical and
theoretical approaches to protein gel electrophoresis.

4.1.1 SDS PAGE Gels

Run Voltage Starting Current Ending Voltage Approx. Run TIme

90VDC 30mA/gel CC 110 volts/gel 4-5 hours

4.1.2 General Recommendations

If running only one gel, keep the volts the same but reduce the mA’s by half. Keep in
mind that as the thickness of gel increases, the mA’s increase proportionally.

At constant voltage, the proteins will migrate at a constant rate during electropho­resis with adequate heating appropriate for denaturing gels. Increasing the voltage/
mA (for a single gel thickness and percentage) will speed mobility but increase the
risk of overheating.

If using cooling base the power input and the migration rate can be increased. The
joule heating generated by the higher power is offset by the cooling effect of the
buffer between the gels.

4.2 References

1. Hames, B.D. (ed.) (1998). Gel Electrophoresis of Proteins. A Practical
Approach. 3

2. Sambrook, J., Fritsch, Russell, D. (2001). Molecular Cloning. A Laboratory
Manual. 3

New York. A8.40-A8.55

3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith,
J.A., Struhl, K. (ed) (1993). Current Protocols in Molecular Biology. Vol. 2,
Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., Ch. 10.

rd

edn. Oxford University Press, Oxford. Ch. 1,3.

rd

edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor,

14

DCX-900C Large Format Instructions 9-30-13

SECTION 5
Maintenance of Equipment

5.1 Care and Handling

The plastic components of the Large Format Cooled Vertical Electrophoresis Systems

are fabricated from acrylic and polycarbonate. Electrodes and connectors are made
from pure platinum, stainless steel, and chrome plated brass. As with any laboratory
instrument, adequate care ensures consistent and reliable performance.

After each use, rinse buffer chamber, gel tray and combs with de-ionized water. Wipe
dry with a soft cloth or paper towel, or allow to air dry. Whenever necessary, all compo­nents may be washed gently with water and a non-abrasive detergent, and rinsed and

dried as above. Never use abrasive cleaners, glass cleaning sprays or scouring pads to

clean the components, as these will damage the unit and components.

Additional precautions:

• Do not autoclave or dry-heat sterilize the apparatus or components.

• Do not expose the apparatus or components to phenol, acetone, benzene,

halogenated hydrocarbon solvents or alcohols.

• Avoid prolonged exposure of the apparatus or components to UV light.

• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended
periods at 37°C. A brief rinse with DEPC-water is sufcient after a thorough
wash, followed by a quick rinse in 50% ethanol.

5.2 Maintenance

The following inspection and maintenance procedures will help maintain the safety and

reliable performance of the Large Format Cooled Vertical Systems. Replacement parts
can be ordered by calling 1-858-755-4959 or by contacting your local distributor.

• Banana plugs and power cords should be inspected regularly. If the banana

plugs become loose or do not feel friction tight replace the plugs or power
cords.

• Should power cord assemblies (connectors, wire or shrouds) show any signs

of wear or damage (e.g. cracks, nicks, abrasions, or melted insulation), replace
them immediately.

• The platinum wire is secured to the banana jack by compression between a

stainless washer and the jack nut. The nut/washer interface should be tight
and free of corrosion.

15

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SECTION 6

Ordering Information

Large Format Cooled Vertical Ordering Information

COMBS: See page 6.

CAT. # DESCRIPTION

COOLED LARGE FORMAT GEL SYSTEM- 20CM(W)*

DCX-900C

Accessories and Related Products for Cooled Large Format Gel System

SGP20-020R Gel Wrap
SGP20-020R-DN Gel Wrap
VGE20-0720 Gel Wrap
VGE20-1020 Gel Wrap
VGE20-1520 Gel Wrap
SGS-20-0720R Gel Wrap
SGS-20-1020R Gel Wrap
SGS-20-1520R Gel Wrap
DCX9-MC-4
SGP20-020 Multi-Caster Glass Plate Set
SGP20-020-DN Multi-Caster Glass plate Set with Borosilicate back plate
SGS-20-0720 Multi-Caster Spacer Set, 0.75mm thick
SGS-20-1020 Multi-Caster Spacer Set, 1.0mm thick
SGS-20-1520 Multi-Caster Spacer Set, 1.5mm thick
EBU-222
EBC-222 Additional Blotting Cassette for EBU-222
EBU-4000
EPS-600
EPS-3000P-II

Cooled Large Format Gel System, CE, Complete System, glass plate dimensions 20cm(w)
x 22cm(l)

®

Glass Plate Set

®

Glass plate Set with Borosilicate back plate

®

Gasket, 0.75mm thick

®

Gasket, 1.0mm thick

®

Gasket, 1.5mm thick

®

Spacer Set, 0.75mm thick

®

Spacer Set, 1.0mm thick

®

Spacer Set, 1.5mm thick

DCX-900 Multi-Caster, 4-place, Unit Only, fits 4 sets of glass plates 20cm(w) x 22cm(l)

Large Blotting System, 2-place, fits gel up to 20cm(w) x 20cm(l). Includes 1 gel cassette.

Semi-Dry Blotting System, transfer area of 20cm x 20cm
Power Supply, CE, 5-600V, 3-500mA
High Voltage Power Supply, CE, 20-3000V, 1-400mA

16

DCX-900C Large Format Instructions 9-30-13

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18

DCX-900C Large Format Instructions 9-30-13

19

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CONTACT INFORMATION

Online Ordering

www.cbsscientic.com

Telephone

Local and International: 858-755-4959
Toll Free: 800-243-4959

Sales E-mail Address

[e-mail]

Technical Service E-mail Address

[e-mail]

Mailing Address

P.O. Box 856
Del Mar, CA 92014

Shipping Address

10805 Vista Sorrento Parkway, Ste 100
San Diego, CA 92121