INSTRUCTION MANUAL
Adjustable Slab Gel Systems
Single Units
ASG-250
ASG-400
ASG-250-02
Dual Units
DASG-250
DASG-400
DASG-250-02
www.cbsscientific.com 2 Adjustable Slab Gel Manual 07/13
TABLE OF CONTENTS
Page
Important User Information 3-4
Section 1 General Information
1.1 Introduction 5
1.2 Specifications 5-6
1.3 Safety 7
Section 2 Description of parts
2.1 Unpacking-U.S. version 8
2.2 Components/Assembly-U.S. version 8
2.3 Unpacking-European version 9
2.4 Components/Assembly-European version 9
Section 3 Instructions for Use
3.1 Adjustable Slab Gel Unit Preparation 10
3.2 Preparation/Cleaning of glass plates 11
3.3 Gel Casting 11-12
3.4 Running the Gel 13-14
3.5 Removing the Gel 14
3.6 Maximum Well/Comb volumes 14
Section 4 Running Conditions
4.1 Recommended Power 15
4.2 Recommended Buffer & Reagents 15-16
4.3 References 16
Section 5 Maintenance of Equipment
5.1 Care and Handling 17
5.2 Maintenance 17
Section 6 Adjustable/Dual Adjustable Slab Gel
Kits and Accessories 18-21
www.cbsscientific.com 3 Adjustable Slab Gel Manual 07/13
IMPORTANT USER INFORMATION
This Instruction Manual will explain how to use this product safely and effectively. Please read and
carefully follow the instruction manual in its entirety.
The triangle/exclamation mark symbol alerts the user of the product to important
operational, maintenance, and/or warranty requirements.
The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous
electrical exposure.
Failure to adhere to the instructions could result in personal and/or laboratory hazards, as well as
invalidate any warranty. Always turn off the DC power source prior to disconnecting power cords
from the product. Disconnect power cords from the power source first, and then from the product.
For maximum safety, always operate this system in an isolated, low traffic area, not accessible to
unauthorized personnel. Never operate damaged or leaking equipment.
WARRANTY AND LIABILITY
This product was produced utilizing the highest practical standards of materials, workmanship, and
design. C.B.S. Scientific warrants that the product has been tested and will meet or exceed published
specifications. This warranty is valid only if the product has been operated and maintained according to
the instructions provided.
C.B.S. Scientific warrants this product to be free from defects in materials and workmanship under normal
service for one year from date of shipment. If the product proves defective during this period, C.B.S.
Scientific will repair or replace it at our option, free of charge, if returned to us postage prepaid. This
warranty does not cover: damage in transit, damage caused by carelessness, misuse or neglect, normal
wear through frequent use, damage caused by solvent corrosion, damage caused by improper handling
or user alteration, nor unsatisfactory performance as a result of conditions beyond our control. C.B.S.
Scientific shall in no event be liable for incidental nor consequential damages, including without limitation,
lost profits, loss of income, loss of business opportunities, loss of use and other related damages,
however caused, nor any damage arising from the incorrect use of the product.
www.cbsscientific.com 4 Adjustable Slab Gel Manual 07/13
FRANÇAIS INFORMATION IMPORTANTE À L'USAGE DES UTILISATEURS
Le présent manuel d'utilisation explique la manière de se servir efficacement du produit en conditions
de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes
et ses instructions.
Le triangle avec poin t d'exclamation est un symbole destiné à avertir l'utilisateur du produit
de l'importance de certaines exigences relatives au fonctionnement, à l'entretien et/ou à la
garantie.
Le triangle avec flèche en zigzag est un symbole destiné à avertir l'utilisateur du produit de la
possibilité d'exposition à des décharges avec danger de secousses électriques.
Tout manquement à l'observation des consignes et des instructions peut exposer les personnes
et les biens à des dommages corporels et/ou matériels et peut annuler toute garantie. Il faut toujo urs
interrompre l'alim entation de coura nt continu avant de déconnecter les cordons d'alimen tation du
produit. Déconnecter d'abord les cordons d'alimentation branchés sur la source de tension
(alimentation de secteur) puis ceux branchés sur le produit. Pour une sécurité maximum, il faut
toujours faire fonctionner ce système dans un lieu isolé, peu fré quenté, où le pe rsonnel non autorisé
n'a pas accès. Ne jamais faire fonctionner un matériel endommagé ou affecté par des fuites.
GARANTIE ET RESPONSABILITÉ
Le produit a été fabriqué conformément aux normes applicables les plus exigentes en matière de
matériaux, de main d'oeuvre, de conception et d'ingéniérie. C.B.S. Scientific garantit que le produit a
subi des essa is et que ses performances rempliront les conditions des spécifications publiées ou leur
seront même supérieures. La présente garantie n'est valide que si le produit a fonctionné et a été
entretenu conformément aux consignes et instructions fournies.
C.B.S. Scientific garantit que le produit sera dépourvu de vices de matériaux et de main d'oeu vre, en
conditions de service normales, pendant un an à compter de la date d'expédition. Au ca s où le produit
s'avérerait défectueux pendant cette période de garantie, C.B.S. Scientific réparera ou remplacera le
produit, à sa discrétion et gratuitement, si le produit lui est retourné port payé d'avance. La garantie ne
couvre pas les dommages de transport; les dommages causés par l'imprudence, le manque de soins,
l'abus ou la négligence; l'usure normale résultant d'une utilisation fréquente; les dommages causés par
la cor rosion des so lvants; et les dom mages causés par la man ipulation inadéquate o u de s
changements apportés par l'utilisateur. La garantie ne couvre pas non plus les performances non
satisfaisantes résultant de conditions hors du contrôle de C.B.S. Scientific. C.B.S. Scientific ne pourra
en aucun cas être tenue responsable de dommages indirects, y compris, de manière non limitative, la
perte de bénéfices, le manque à gagner, la perte d'occas ions d'affaires, l'im possibilité d'usage ou tous
autres dommages associés, quelle qu'en soit la cause, ni de dommages résultant de l'usage incorrect
du produit.
ESPAÑOL INFORMACIÓN IMPORTANTE PARA EL USUARIO
El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase
leerlo en su totalid ad y seguir detenidamente la s indicaciones q ue contiene.
El símbolo del triángulo con exclamación llama la atención del usuario a requisitos
importantes pa ra el uso y mantenimiento de l producto, así como para la validez de la
garantía.
El símbolo del triángulo con rayo llama la atención del usuario a la posibilidad de
riesgos eléctricos.
El incumplimiento de las instrucciones aquí señaladas podría dar lugar a riesgos a la persona, al
laboratorio o a ambos y podría an ular toda garantía. Siempre apague la fuente de corriente continua
antes de desenchufar los cables eléctricos del producto. Primero desconecte los cables de la fuente
de energía y después del prod ucto. Pa ra mayor seguridad, siempre use este sis tema en un área
aislada, de poco movimiento de personas e inaccesible a personal no autorizado. Jamás use equipo
que presenta algún daño o fuga.
GARANTÍA Y RESPONSABILIDAD
Este producto fue fabricado de acuerdo con las normas más estrictas que sean factibles en cuanto a
materiales, mano de obra y diseño. C.B.S Scientific garantiza que se sometió el producto a pruebas y
que cumplirá o excederá las especificaciones publicadas. E sta garantía será válida únicamente si se
usa y se da servicio de mantenimiento al producto de acuerdo con las instrucciones señaladas.
C.B.S. Scientific garantiza que este producto se encontrará libre de defectos de materiales y mano de
obra por un período de servicio normal de un año a partir de la fecha de embarque. Si el producto
resulta defectuoso durante este período, C.B.S. Scientific lo re parará o lo repondrá, a criterio de
C.B.S., libre de cargos, si se devuelve el producto a C.B.S. porte pagado. Esta garantía no cubre
daños sufridos en tránsito, daños provocados por descuido, mal uso o negligencia, desgaste normal
como con secuencia del uso excesivo, daños atribuibles a corrosión provocada por solventes, daños
causados por el uso indebido o alteraciones realizadas por el usuario ni rendimiento insatisfactorio
atribuible a circunstancias fuera del control de C.B.S. Scientific. C.B.S. Scientific en ningún caso
asumirá responsabilidad por daños incidentales o subsecuentes, incluyendo, en forma no limitativa, la
pérdida de utilidades, de ingresos, de oportunidades comerciales o del uso del producto y otros daños
afines, fuere cual fuere su origen, ni por daños derivados del uso incorrecto del producto.
DEUTSCH WICHTIGE INFORMATION FÜR DEN BENUTZER
Diese Bedienungsanleitung beschreibt wie man dieses Produkt sicher und wirksam benutzt. Bitte
lesen und befo lgen Sie alle An weisungen in d ieser Anleitung .
Das Dreieck mit Ausrufezeichen weist den Benutzer des Produktes darauf hin, daß
wichtige Bedienungs-, Wartungs- un d/oder Garantievo rschriften zu beachte n sind.
Das Dreieck mit Zickzackblitz warnt den Benutzer des Produktes vor möglichen
Gefahren durch elektrische Spannungen.
Nichtbeachtung dieser Anweisungen kann zu persönlichen und/oder labortechnischen Schäden
führen und g leichzeitig alle Ga rantien als nich tig erklä ren. Die DC Stromzufuhr muß immer, vor dem
Entfernen der Stromkabel vom Produkt, abgeschaltet werden. D ie Stromzufuhrkabel müssen zuerst
von der Steckdose und erst dann vom Produkt entfernt werden. Um höchste Sicherhe it zu
gewährleisten sollte dieses System in einem abgesonderten und besonders ruhigen Bereich eingesetzt
werden und vor Unbefugten sicher sein.
GARANTIE UND HAFTUNG
Dieses Produkt wurde unter Anwendung von Produkten mit höchster Qualität und aus Materialien mit
bester Verarbeitung und modernstem Design hergestellt. C.B.S Scientific garantiert, daß das Produkt
getestet w urde und alle publizierten Spezifikationen übertrifft. Diese Garantie ist jedoch nur gültig,
wenn das Produkt nach der beigefügten Bedienungsanleitung bedient und gewartet wurde.
C.B.S. Scientific garantiert, daß dieses Produkt bei normaler Bedienung aus fehlerfreiem Material
besteht und fehlerfrei in der Ausführung ist. Diese Garantie gilt für ein Jahr ab Lieferdatum. Sollte das
Produkt in diesem Zeiraum fehlerhaft werden, bietet C.B.S. Scientific eine kostenlose Reparatur bzw.
kostenlosen Ersatz, einschließlich freiem Rückporto. Diese Garantie schließt folgendes aus:
Transportschaden, Schaden durch Nachlässigkeit, Mißbrauch oder Vernachlässigung, normale
Abnützung durch regelmäßigen Gebrauch, Schaden durch Säureangriff, Schaden durch falsche
Handhabung, Veränderung des Produktes durch den Benutzer, oder unzureichende Leistungen die
sich nicht im Verantwortungsbereich von C.B.S. Scientific befinden. C.B.S. Scientific kommt unter
keinen Umständen für folgende Schäden auf: Sachschadensverlust, Einkommensverlust, Verlust von
Geschäftsmöglichkeiten, Verlust der Anwendung und andere damit verbundene Schäden die auf
irgend eine Art un d Weise entstanden sind, o der Schäden die aus falscher Anwen dung des Produkte s
entstanden sind.
ITALIANO INF ORMAZIONI IM PORTANTI PER L’UTENTE
Questo manuale spiega come utilizzare questo prodotto in maniera sicura ed efficiente. Si pregai di
leggere e segu ire con cautela le istruzioni di ogni parte di questo m anuale.
Il triangolo contenete il s imbolo di un punto esclamativo avver te l’utente di importanti
requisiti relativi al funzionamento, man utenzione e/o garanzia del prodotto.
Il triangolo contenete il s imbolo di un lampo avverte l’utente del p rodotto della
possibilità di pericoli dovuti a corrente elettrica.
La mancata osservanza delle istruzioni può essere causa di pericolo alla propria persona ed al
laboratorio, oltre a poter annullare la garanzia. Prima di distaccare il cordone d’alimentazione dal
prodotto, spegnere sempre la sorgente di corrente continua. Distaccare i cordoni d’alimentazione
prima dal lato della sorgente di tensione e poi dal lato del prodotto. Per maggior sicurezza, mettere
sempre in funzione il prodotto in un’area isolata con poco traffico che non sia accessibile al personale
non autorizzato. Non mettere mai in funzione un’apparecchiatura che sia danneggiata o abbia
perdite.
GARANZIA E RESPONSABILITA
Questo prodotto è stato fabbricato seguendo gli standard più elevati per i materiali, la manodopera e la
progettazione. La C.B.S. Scientific garantisce il prodotto è stato sottoposto a prova e raggiunge o
supera i valori pubblicati per i dati tecnici. Questa garanzia è valida solo se il prodotto è messo in
esercizio e soggetto a manutenzione secondo le istruzioni fornite.
La C.B.S. Scientific garantisce che questo prodotto è libero di difetti di materiali e manodopera, in
normali condizioni d’esercizio, per la durata di un anno dalla data di spedizione. Se, in questo periodo,
il p rodotto si dimostre rà difettoso, la C.B.S. S cientific, a suo giudizio, lo r iparerà o sostituirà. Questa
garanzia non copre danni in transito, danni causati da negligenza, uso improprio, trascuratezza,
normale consumo derivante da uso frequente, o danni causati da solventi corrosivi, danni causati da
maltrattamento o da modifiche apportate dall’utente e non copre prestazioni insoddisfacenti che siano il
risultato di cond izioni al di fuori del controllo del fab bricante. La C.B.S. Scientific non sarà in ogni caso
responsabile per da nni incidentali o cons equenziali, incluso, senz a limitazioni, perdite di p rofitto,
perdita di entrate, perdita di opportunità d’affari e altri danni relativi, comunque causati, e per danni
risultati da uso incorretto del prodotto.
www.cbsscientific.com 5 Adjustable Slab Gel Manual 07/13
SECTION 1
General Information
1.1 Introduction
The Adjustable Slab Gel Systems offered by C.B.S. Scientific are designed to meet a wide variety
of applications. These systems are designed for separating and characterizing protein and can
be adapted for short nucleic acid sequencing runs with the appropriate accessories. The Dual
systems allow two gels to be run simultaneously under identical buffer conditions and also
provides the flexibility of running gels from 145mm to 400mm (U.S. version, Cat. # DASG-250 or
DASG-400) or 160mm to 280mm (European version, Cat. # DASG-250-02). The single systems
provide the flexibility of running gels in lengths from 145mm to 400mm (U.S. version, Cat. # ASG250 or ASG-400) or 160mm to 280mm (European version, Cat. # ASG-250-02). Gel casting can
be accomplished using our convenient and unique Gel Wrap, or by using our multiple gel
casting chambers (see Section 6, Adjustable/Dual Adjustable Slab Gel Kits and Accessories).
Other applications include; mobility shift assays, antibody super shift assays, Rnase protection
assays, DNA footprinting, differential display, DNA paternity testing and forensic analysis.
1.2 Specifications
Constructions:
Buffer chamber, safety cover Acrylic
Electrodes Platinum wire .012" diameter
Power cords FR Urethane rated 7500VDC,
200mA, 65°C
Guide Rods Delrin
Combs Teflon
Glass plates Soda-lime float glass
Spacers PVC
Clamps Polypropylene, stainless steel
Safety Certification EN61010-1-1993 (IEC1010-1)
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Table 1 Specifications
Single Adjustable
Unit
U.S. version
ASG-250/400
adjusted to:
Shipping
weight
Overall unit
size cm
(l) x (w) x (h)
Distance between
electrodes mm
Recommended
buffer volume mls
Reservoirs:
UPPER LOWER
Voltage Limit
VDC
145mm
7 lb.
24 x 19 x 21.91
250
310-375 150-350
725
160mm
7 lb.
24 x 19 x 31
265
310-375 150-350
800
170mm
7 lb.
24 x 19 x 31
275
310-375 150-350
850
190mm
7 lb.
24 x 19 x 31
295
310-375 150-350
950
220mm
7 lb.
24 x 19 x 31
325
310-375 150-350
1100
280mm
8 lb.
24 x 19 x 31
385
310-375 150-350
1400
400mm
10 lb.
24 x 19 x 41
505
310-375 150-350
2000
Single Adjustable
Unit
European version
ASG-250-02
adjusted to:
Shipping
weight
Overall unit
size cm
(l) x (w) x (h)
Distance between
electrodes mm
Recommended
buffer volume mls
Reservoirs:
UPPER LOWER
Voltage Limit
VDC
160mm
7 lb.
24 x 19 x 31
265
250-300 100-200
800
170mm
7 lb.
24 x 19 x 31
275
250-300 100-200
850
190mm
7 lb.
24 x 19 x 31
295
250-300 100-200
950
220mm
7 lb.
24 x 19 x 31
325
250-300 100-200
1100
280mm
8 lb.
24 x 19 x 31
385
250-300 100-200
1400
Dual Adjustable Unit
U.S. version
DASG-250/400
adjusted to:
Shipping
weight
Overall unit
size cm
(l) x (w) x (h)
Distance between
electrodes mm
Recommended
buffer volume mls
Reservoirs:
UPPER LOWER
Voltage Limit
VDC
145mm
12 lb.
24 x 19 x 21.91
250
375-475 150-350
725
160mm
12 lb.
24 x 19 x 31
265
375-475 150-350
800
170mm
12 lb.
24 x 19 x 31
275
375-475 150-350
850
190mm
12 lb.
24 x 19 x 31
295
375-475 150-350
950
220mm
12 lb.
24 x 19 x 31
325
375-475 150-350
1100
280mm
13 lb.
24 x 19 x 31
385
375-475 150-350
1400
400mm
14 lb.
24 x 19 x 41
505
375-475 150-350
2000
Dual Adjustable Unit
European version
DASG-250-02
adjusted to:
Shipping
weight
Overall unit
size cm
(l) x (w) x (h)
Distance between
electrodes mm
Recommended
buffer volume mls
Reservoirs:
UPPER LOWER
Voltage Limit
VDC
160mm
12 lb.
24 x 19 x 31
265
300-350 400-600
800
170mm
12 lb.
24 x 19 x 31
275
300-350 400-600
850
190mm
12 lb.
24 x 19 x 31
295
300-350 400-600
950
220mm
12 lb.
24 x 19 x 31
325
300-350 400-600
1100
280mm
13 lb.
24 x 19 x 31
385
300-350 400-600
1400
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1.3 Safety
Power to the Adjustable Vertical units is to be supplied by an external DC voltage power supply
that must be ground isolated so that the DC voltage output floats with respect to ground. For any
power supply used, the maximum specified operating parameters for the units are:
Maximum Limits
50VDC/cm of gel
60 watts power
80mA current
55°C ambient temperature
Current to the unit, provided from the external power supply, must enter the unit through the lid
assembly, providing a safety interlock to the user. Current to the unit is broken when the lid is
removed. Do not attempt to use the unit without the safety lid, and always turn the power
supply off before removing the lid, or when working with the unit in any way. Follow
safety precautions specified by the power supply manufacturer.
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SECTION 2
Description of Parts
2.1 Unpacking—U.S. version only
Please verify that your unit comes complete with the following components:
Single Adjustable Vertical Gel Kit
ASG-250/400:
Dual Adjustable Vertical Gel Kit
DASG-250/400:
• Adjustable electrophoresis unit
• Dual Adjustable electrophoresis unit
• Safety cover with attached DC power leads
• Safety cover with attached DC power leads
• Black guide rods with stainless screws
• Black guide rods with stainless screws
• 2ea GPC-0001, white spring clamps
• 4ea GPC-0001, white spring clamps
• 6ea GPC-0002, white spring clamps
• 12ea GPC-0002, white spring clamps
• 1ea comb
• 2ea combs
• 1 set glass plates
• 2 sets glass plates
• 1 set spacers
• 2 sets spacers
• 1ea Gel Wrap
• 2ea Gel Wrap
• Bubble level, attached to base
• Bubble level, attached to base
• 2ea black thumb screws
• 2ea black thumb screws
• 4ea leveling screws, nylon
• 4ea leveling screws, nylon
2.2 Components/Assembly-- U.S. version only
Single Adjustable Vertical Gel Kit Dual Adjustable Vertical Gel Kit
www.cbsscientific.com 9 Adjustable Slab Gel Manual 07/13
SECTION 2---continued
Description of Parts
2.3 Unpacking—European version only
Please verify that your unit comes complete with the following components:
Single Adjustable Vertical Gel Kit
ASG-250-02:
Dual Adjustable Vertical Gel Kit
DASG-250-02:
• Adjustable electrophoresis unit
• Dual Adjustable electrophoresis unit
• Safety cover with attached DC power leads
• Safety cover with attached DC power leads
• Black guide rods with stainless screws
• Black guide rods with stainless screws
• 2ea GPC-0001, white spring clamps
• 4ea GPC-0001, white spring clamps
• 6ea GPC-0002, white spring clamps
• 12ea GPC-0002, white spring clamps
• 1ea comb
• 2ea combs
• 1 set glass plates
• 2 sets glass plates
• 1 set spacers
• 2 sets spacers
• 1ea Gel Wrap
• 2ea Gel Wrap
• Bubble level, attached to base
• Bubble level, attached to base
• 2ea black thumb screws
• 2ea black thumb screws
• 4ea leveling screws
• 4ea leveling screws
2.4 Components/Assembly—European version only
Single Adjustable Vertical Gel Kit Dual Adjustable Vertical Gel Kit
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Section 3
Instructions for Use
3.1 Adjustable Slab Gel Unit Preparation
A. Unit Set-Up
1. Place Adjustable Slab Gel unit on a level work surface in an authorized work area.
Attach each of the black guide rods to the flathead screws through the countersunk
holes on the bottom of the base. Thread the screws into the black guide rods and
hand tighten. Do not over tighten.
2. Slide the upper reservoir onto the guide rods. Be sure to unscrew the thumbscrews
slightly so that the ends of the screws are not protruding into the guide rod channels.
3. Adjust the upper reservoir so that the facia and glass notches are level, using a
notched plate as a guide. Tighten the two black thumbscrews to fix the height.
4. Level the unit by adjusting the four white nylon thumbscrews until the bubble is
centered in the level. Be sure that all four of the screws are touching the bench.
5. Verify that the comb, spacer set and Gel Wrap gasket are the same thickness by
assembling without gel casting.
B. Adjustable-Height Safety Cover (European version only).
The safety cover is shipped set at a height corresponding to the 160mm glass plates. To
adjust the cover height:
1. Pull both white handles outward to release tabs.
2. Immediately push up on inner cover with index finger, to increase height, until tab
reaches desired slot. Alternatively, invert both section of the adjustable height safety
cover, pull both white handles outward to release tabs, allowing the inner section to
slide to desired height.
Reminder: For proper safety cover operation, height of upper reservoir notch
must align with the notch in the glass plates. Place a single notched glass
plate (or short plate if using unnothced sets) on to feet in lower reservoir and
adjust upper reservoir until glass and upper reservoir notch are even (see step
3 in Unit Set-up). Secure black thumbscrews. Test fit safety cover before using
system.
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3.2 Preparation/Cleaning of Glass Plates
Hand wash both plates with a high quality lab detergent followed by a complete rinsing
with dH
2
O. Air dry or use a lint-free tissue. Spray/wipe the chosen inner surfaces of the
plate set with 95% ethanol and dry with lint-free tissue.
3.3 Gel Casting Using Gel Wrap Gasket Casting method
1. Start by holding the rectangular back plate with
the rounded bottom corners and start applying
the gasket around one side of the glass plate.
Note: one side of the “U” shaped gasket is flat,
and the other side has tubing that will act as a
seal around the spacers.
2. When applying the gasket over the rounded
corners of the back glass plate, make sure the
notches on the gasket align with the rounded
corners of the glass plate. Once the gasket is
pushed over the bottom edge and corners, work
it down the remaining side.
3. Place the gasketed plate on the lab bench with
the tubing side up, and extend the bottom of the
plate over the edge of the bench, approximately
¾ of an inch. Place the spacers along side the
inside edges of the gasket. Be sure the rounded
corner end of each spacer is facing the outside
bottom of the plate, following the radius of the
glass.
4. Place the notched plate on top of the bottom
assembly, starting from the bottom edge and
gently easing the plate down. Verify the gasket is
smooth around the edges and then clamp along
the bottom.
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5. Lift the assembly and stand it on the base of the
clamps. For leveling, push glass plate assembly
down until it stops against clamp body. Clamp
the sides of the assembly with additional casting
clamps on either side. As each clamp is
attached, be sure the gasket is aligned between
the plates forming a seal.
6. Apply PAGE or agarose solution to gel plate sandwich using a syringe or pipette. If
using a stacking gel, pour desired height of running gel, then overlay a small amount
of dH
2
O or 0.1% SDS solution to top of gel. After polymerization, rinse with buffer,
add stacking gel solution and insert comb. For regular, unit percentage gels, add
polyacrylamide solution to correct height, and insert comb. Allow gel to set, usually
20 minutes. Extra gel solution in pipette or syringe can be monitored to test
polymerization of gel mix.
7. Disassembly. Hold the clamped plate
assembly with one hand. Remove the gasket
by starting at one of the top ends and pulling
up and out on the gasket until it releases from
the plate, up to the bottom of each of the
white clamps. When each clamp is reached
DO NOT remove it, instead feed the gasket
down through the clamp body and repeat
pulling up and out. Continue feeding until the
gasket is fully detached. If gel, is not to be
used immediately, wrap entire plate sandwich
with plastic wrap tightly to seal and store at
4°C for up to a month.
www.cbsscientific.com 13 Adjustable Slab Gel Manual 07/13
3.4 Running the Gel
1. Attach plate set(s) to unit with
smaller or notched plate facing
towards the upper buffer reservoir. If
using a pre-cast gel stored at 4°C,
allow to warm to room temperature.
Use white clamps with mouth open
(cat. # GPC-0001) to attach plate
set to unit, clamping each side to
the sandwich.
1b. Optional Cooling Plate
Instructions:
If using optional aluminum plate
during electrophoresis place behind
the unit shown in figure at right.
Secure in place with 2 each GPC0001 white clamps (ordered
separately).
2. Pour freshly prepared buffer in
upper and lower chamber. Using a
pipette or syringe, thoroughly flush
out the wells in the glass plate
sandwich with buffer from the upper
chamber. Boil or heat samples and
then place immediately on ice. Load
samples. If outer lanes do not
contain sample, it is recommended
that you run standards (Rainbow™
standards can be monitored easily
during run) and/or fill outer lanes
with loading buffer to reduce smiling
and wrap-around effects.
3. Attach safety cover.
4. Connect the leads to the power
supply, matching the color-coded red
to red and black to black. See
Section 4.1 for recommended
power conditions. Begin
separation by electrophoresis
3.5 Removing the Gel
1. Turn the power supply off and disconnect the leads from the power supply. Remove the
safety cover from the unit, by placing thumbs on white posts next to red & black connectors,
then pushing down while pulling up with fingers under lid. DO NOT pull on power cords.
2. Drain upper reservoir using a pipette or syringe. Detach side clamps and remove gel
sandwich. Carefully separate, making sure that the gel remains attached to one plate. Stain
and fix according to your preferred method. Dispose of buffer according to your institutions
safety requirements.
3.6 Maximum Well/Comb Volumes
NOTE: To calculate sample well volume expressed in millimeters (mm) of height divide
maximum volume by tooth depth.
TABLE 1---Combs
Overall
Length
13cm
Tooth
width/
mm
Tooth
Depth
Spacing
between
teeth
mm
0.75mm
thickness
volume per
tooth
microliters
(ul)
1.0mm
thickness
volume per
tooth
microliters
(ul)
1.5mm
thickness
volume per
tooth
microliters
(ul)
2.0mm
thickness
volume per
tooth
microliters
(ul)
3.0mm
thickness
volume per
tooth
microliters
(ul)
1
130.2
19.1
3.2
1865
2486
3729
4966
7458
2
63.5
19.1
3.2
909
1213
1819
2426
3639
3 equal
41.3
19.1
3.2
591
788
1182
1576
2364
2 w/middle
marker
59.8/
4.2
19.1
3.2
856
1142
1713
2284
3426
4
30.1
19.1
3.2
432
576
1151
1152
1728
5
23.5
19.1
3.2
336
449
673
898
1347
6
19.1
19.1
3.2
273
364
546
728
1092
8
13.5
19.1
3.2
193
257
386
514
771
10
10.1
19.1
3.2
145
194
291
388
582
12
7.9
19.1
3.2
113
151
227
302
453
14
6.4
19.1
3.2
91
121
182
242
363
15
5.7
19.1
3.2
82
109
163
218
327
16
5.2
19.1
3.2
74
98
147
196
294
20
4.2
17.7
2.4
55.72
74.3
111.45
148.7
222.9
29
2.9
17.7
1.6
38.5
51.33
77
102.66
154.0
30
2.8
17.7
1.6
37.2
49.6
74.4
99.12
148.8
www.cbsscientific.com 15 Adjustable Slab Gel Manual 07/13
SECTION 4
Running Conditions
4.1 Recommended Power
Precise electrophoresis conditions will vary according to the number and type of gels used, buffer
conditions employed, power input, and the general goal of the experiment. Refer to the reference
section for in depth discussions on practical and theoretical approaches to protein gel
electrophoresis.
Using standard SDS-PAGE buffer systems apply 1-10 VDC/cm of gel. For sequencing
applications use 50 VDC/cm. If running two gels in the Dual Units, keep the volts the same but
double the mA. It is also true that if the thickness of gel increases, increase the mA
proportionally.
At constant voltage, the proteins will migrate at a constant rate during electrophoresis with
adequate heating appropriate for denaturing gels. Increasing the voltage/mA (for a single gel
thickness and percentage) will speed mobility but increase the risk of overheating.
The sample migration rate can be increased by raising the input power. This can be done son
systems which employ “active” temperature control such as Dual Slab Gel Units and Dual MiniVertical Gel Units. The joule heating generated by the higher input power is offset by the cooling
effect of the water jacket between the gels. Exact conditions should be determined empirically
but could be increased at least in the 20% range.
4.2 Recommended Buffers and Reagents
Pre-mixed acrylamide stock solutions are the method of choice. Use according to manufacturer’s
instructions.
Typical ‘scratch’ recipe for a 4% acrylamide gel:
10mls 40% acrylamide
6.6mls 2% Bis-acrylamide stock
5mls 10X TBE
78.4mls dH
2
0
750ul 10% APS
50ul TEMED
1. Make up 0.5X TBE buffer
2. After gel apparatus is set-up and ready for the gel to be poured, add 750ul
fresh 10% APS solution to the acrylamide solution.
3. Add 5 to 10 ul TEMED and using a 10 to 25ml pipette, quickly “pour” the gel.
4. Allow the gel to polymerize at least 60 minutes.
5. Remove the comb after polymerization and wash out wells with 0.5X TBE
(acrylamide will seep into the wells).
6. Fill upper and lower chambers with 0.5X TBE
7. Pre-electrophorese gel, if needed, 20-30 minutes.
8. Load wells with samples.
9. Monitor migration with dye markers.
www.cbsscientific.com 16 Adjustable Slab Gel Manual 07/13
4.2 Recommended Buffers and Reagents-continued
BUFFERS:
Agarose Page/Agarose Slab
TBE (1X solution):
TAE (1X):
0.089M Tris base 0.04M Tris-acetate
0.89M Boric acid 0.001M EDTA
0.002M EDTA pH 8.0
pH 8.3
Protein Denaturing Denaturing/Non-Denaturing/
TG-SDS(1X):
Nylon Blotting
0.025M Tris base TT (Tris-Taurine( (1X):
TT-SDS (1X):
0.192M Glycine 0.1M Tris base 0.1M Tris base
0.1%(w/v) SDS 0.1M Tricine 0.1M Tricine
pH3 0.1%(w/v) SDS
DNA Sequencing
TTE (Tris/Taurine/EDTA)(1X):
1.78M Tris
0.57M Taurine
0.01M EDTA Na2-2H
2
0
4.3 References
Hames, B.D., Rickwoood, D. (ed.) (1990). Gel Electrophoresis of Proteins. A Practical Approach.
2
nd
edn. IRL Press, Oxford. Ch.1 & 3.
Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. 2
nd
edn.
Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New
York. 18.47-18.61.
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A.,
Struhl, K. (ed)
(1993). Current Protocols in Molecular Biology. Vol. 2, Greene Publishing Associates,
Inc. and John Wiley & Sons, Inc., Ch.10.
www.cbsscientific.com 17 Adjustable Slab Gel Manual 07/13
SECTION 5
Maintenance of Equipment
5.1 Care and Handling
The plastic components of the Adjustable Slab Gel units are fabricated from acrylic, delrin and
polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and
chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and
reliable performance.
After each use, rinse buffer chamber, glass plate, spacers and combs with de-ionized water.
Wipe dry with a soft cloth or paper towel, or allow to air dry. Whenever necessary, all
components may be washed gently with water and a non-abrasive detergent, and rinsed and
dried as above. Never use abrasive cleaners, glass cleaning sprays or scouring pads to clean
the components, as these will damage the unit and components.
Additional precautions:
• Do not autoclave or dry-heat sterilize the apparatus or components.
• Do not expose the apparatus or components to phenol, acetone, benzene, halogenated
hydrocarbon solvents or undiluted alcohols.
• Avoid prolonged exposure of the apparatus or components to UV light.
• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended periods at
37°C. A brief rinse with DEPC-water is sufficient after a thorough wash.
5.2 Maintenance
The following inspection and maintenance procedures will help maintain the safety and reliable
performance of the Adjustable Slab Gel systems. Replacement parts can be ordered by calling
1-858-755-4959 or by contacting your local distributor.
• Banana plugs and power cords should be inspected regularly. If the banana plugs
become loose or do not feel friction tight replace the plugs or power cords.
• Should power cord assemblies (connectors, wire or shrouds) show any signs of wear or
damage (e.g. cracks, nicks, abrasions, or melted insulation), replace them immediately.
• The platinum wire is secured to the banana jack by compression between a stainless
washer and the jack nut. The nut/washer interface should be tight and free of corrosion.
www.cbsscientific.com 18 Adjustable Slab Gel Manual 07/13
SECTION 6
Adjustable/Dual Adjustable Slab Gel Kits and Accessories
Cat. # Item
ASG-250 Adjustable Slab Gel Kit, U.S. only. Adjusts from 145mm to 280mm. Includes 1 comb, 1 Gel Wrap
spacer set, 1 Gel Wrap Glass plate set, 1 each Gel Wrap and 8 clamps.
ASG-250-02 Adjustable Slab Gel Kit-CE, adjusts from 160mm to 280mm. Includes 1 comb, 1 Gel Wrap spacer
set, 1 Gel Wrap Glass plate set, 1 each Gel Wrap and 8 clamps.
ASG-400 Adjustable Slab Gel Kit, U.S. only. Adjusts from 145mm to 400mm. Includes 1 comb, 1 Gel Wrap
spacer set, 1 Gel Wrap Glass plate set, 1 each Gel Wrap and 8 clamps..
DASG-250 Dual Adjustable Slab Gel Kit, U.S. only. Adjusts from 145mm to 280mm. Includes 2 combs, 2
Gel Wrap spacer sets, 2 Gel Wrap Glass plate sets, 2 each Gel Wrap and 16 clamps.
DASG-250-02 Dual Adjustable Slab Gel Kit-CE, adjusts from 160mm to 280mm. Includes 2 combs, 2 Gel Wrap
spacer sets, 2 Gel Wrap Glass plate sets, 2 each Gel Wrap and 16 clamps.
DASG-400 Dual Adjustable Slab Gel Kit, U.S. only. Adjusts from 145mm to 400mm. Includes 2 combs, 2
Gel Wrap spacer sets, 2 Gel Wrap Glass plate sets, 2 each Gel Wrap and 16 clamps.
Accessories:
COMBS (Teflon) COMBS (Teflon)
Cat. # Comb dimensions
Cat. # Comb dimensions
VGC-7501 0.75mm x 1 well VGC-2001 2.0mm x 1 well
VGC-7502 0.75mm x 2 well VGC-2002 2.0mm x 2 well
VGC-7503E 0.75mm x 3 equal wells VGC-2003E 2.0mm x 3 equal wells
VGC-7503M 0.75mm x 2 well + middle marker VGC-2003M 2.0mm x 2 well + middle marker
VGC-7504 0.75mm x 4 well VGC-2004 2.0mm x 4 well
VGC-7505 0.75mm x 5 well VGC-2005 2.0mm x 5 well
VGC-7506 0.75mm x 6 well VGC-2006 2.0mm x 6 well
VGC-7508 0.75mm x 8 well VGC-2008 2.0mm x 8 well
VGC-7510 0.75mm x 10 well VGC-2010 2.0mm x 10 well
VGC-7512 0.75mm x 12 well VGC-2012 2.0mm x 12 well
VGC-7514* 0.75mm x 14 well VGC-2014* 2.0mm x 14 well
VGC-7515 0.75mm x 15 well VGC-2015 2.0mm x 15 well
VGC-7516 0.75mm x 16 well VGC-2016 2.0mm x 16 well
VGC-7520 0.75mm x 20 well VGC-2020 2.0mm x 20 well
VGC-7529* 0.75mm x 29 well VGC-2029* 2.0mm x 29 well
VGC-7530 0.75mm x 30 well VGC-2030 2.0mm x 30 well
VGC-1001 1.0mm x 1 well VGC-3001 3.0mm x 1 well
VGC-1002 1.0mm x 2 well VGC-3002 3.0mm x 2 well
VGC-1003E 1.0mm x 3 equal wells VGC-3003E 3.0mm x 3 equal wells
VGC-1003M 1.0mm x 2 well + middle marker VGC-3003M 3.0mm x 2 well + middle marker
VGC-1004 1.0mm x 4 well VGC-3004 3.0mm x 4 well
VGC-1005 1.0mm x 5 well VGC-3005 3.0mm x 5 well
VGC-1006 1.0mm x 6 well VGC-3006 3.0mm x 6 well
VGC-1008 1.0mm x 8 well VGC-3008 3.0mm x 8 well
VGC-1010
1.0mm x 10 well VG
C-3010 3.0mm x 10 well
VGC-1012 1.0mm x 12 well VGC-3012 3.0mm x 12 well
VGC-1014* 1.0mm x 14 well VGC-3014* 3.0mm x 14 well
VGC-1015 1.0mm x 15 well VGC-3015 3.0mm x 15 well
VGC-1016 1.0mm x 16 well VGC-3016 3.0mm x 16 well
VGC-1020 1.0mm x 20 well VGC-3020 3.0mm x 20 well
VGC-1029* 1.0mm x 29 well VGC-3029* 3.0mm x 29 well
VGC-1030 1.0mm x 30 well VGC-3030 3.0mm x 30 well
VGC-1501 1.5mm x 1 well VGC-6001 6.0mm x 1 well
VGC-1502 1.5mm x 2 well VGC-6002 6.0mm x 2 well
VGC-1503E 1.5mm x 3 equal wells VGC-6003E 6.0mm x 3 equal wells
VGC-1503M 1.5mm x 2 well + middle marker VGC-6003M 6.0mm x 2 well + middle marker
VGC-1504 1.5mm x 4 well VGC-6004 6.0mm x 4 well
VGC-1505 1.5mm x 5 well VGC-6005 6.0mm x 5 well
VGC-1506 1.5mm x 6 well VGC-6006 6.0mm x 6 well
VGC-1508 1.5mm x 8 well VGC-6008 6.0mm x 8 well
VGC-1510 1.5mm x 10 well VGC-6010 6.0mm x 10 well
VGC-1512 1.5mm x 12 well VGC-6012 6.0mm x 12 well
VGC-1514* 1.5mm x 14 well VGC-6014 * 6.0mm x 14 well
VGC-1515 1.5mm x 15 well VGC-6015 6.0mm x 15 well
VGC-1516 1.5mm x 16 well VGC-6016 6.0mm x 16 well
VGC-1520 1.5mm x 20 well VGC-6020 6.0mm x 20 well
VGC-1529* 1.5mm x 29 well VGC-6029* 6.0mm x 29 well
VGC-1530 1.5mm x 30 well VGC-6030 6.0mm x 30 well
* Designed for multi-channel pipettor loading.
www.cbsscientific.com 19 Adjustable Slab Gel Manual 07/13
SPACERS for GEL WRAP (PVC) GEL WRAP GASKET
Cat. # Spacer dimensions
Cat. # Gasket thickness x plate size
VGS-0512R 0.50mm x 145mm VGE-0512 0.50mm thick x 145mm
VGS-0516R 0.50mm x 160mm VGE-0516 0.50mm thick x 160mm
VGS-0517R 0.50mm x 170mm VGE-0517 0.50mm thick x 170mm
VGS-0519R 0.50mm x 190mm VGE-0519 0.50mm thick x 190mm
VGS-0520R 0.50mm x 220mm VGE-0520 0.50mm thick x 220mm
VGS-0525R 0.50mm x 280mm VGE-0525 0.50mm thick x 280mm
VGS-0540R 0.50mm x 387mm VGE-0540 0.50mm thick x 387mm
VGS-7512R 0.75mm x 145mm VGE-7512 0.75mm thick x 145mm
VGS-7516R 0.75mm x 160mm VGE-7516 0.75mm thick x 160mm
VGS-7517R 0.75mm x 170mm VGE-7517 0.75mm thick x 170mm
VGS-7519R 0.75mm x 190mm VGE-7519 0.75mm thick x 190mm
VGS-7520R 0.75mm x 220mm VGE-7520 0.75mm thick x 220mm
VGS-7525R 0.75mm x 280mm VGE-7525 0.75mm thick x 280mm
VGS-7540R 0.75mm x 387mm VGE-7540 0.75mm thick x 387mm
VGS-1012R 1.0mm x 145mm VGE-1012 1.0mm thick x 145mm
VGS-1016R 1.0mm x 160mm VGE-1016 1.0mm thick x 160mm
VGS-1017R 1.0mm x 170mm VGE-1017 1.0mm thick x 170mm
VGS-1019R 1.0mm x 190mm VGE-1019 1.0mm thick x 190mm
VGS-1020R 1.0mm x 220mm VGE-1020 1.0mm thick x 220mm
VGS-1040R 1.0mm x 387mm VGE-1040 1.0mm thick x 387mm
VGS-1512R 1.5mm x 145mm VGE-1512 1.5mm thick x 145mm
VGS-1516R 1.5mm x 160mm VGE-1516 1.5mm thick x 160mm
VGS-1517R 1.5mm x 170mm VGE-1517 1.5mm thick x 170mm
VGS-1519R 1.5mm x 190mm VGE-1519 1.5mm thick x 190mm
VGS-1520R 1.5mm x 220mm VGE-
1520 1.
5mm thick x 220mm
VGS-1525R 1.5mm x 280mm VGE-1525 1.5mm thick x 280mm
VGS-1540R 1.5mm x 387mm VGE-1540 1.5mm thick x 387mm
VGS-2012R 2.0mm x 145mm VGE-2012 2.0mm thick x 145mm
VGS-2016R 2.0mm x 160mm VGE-2016 2.0mm thick x 160mm
VGS-2017R 2.0mm x 170mm VGE-2017 2.0mm thick x 170mm
VGS-2019R 2.0mm x 190mm VGE-2019 2.0mm thick x 190mm
VGS-2020R 2.0mm x 220mm VGE-2020 2.0mm thick x 220mm
VGS-2025R 2.0mm x 280mm VGE-2025 2.0mm thick x 280mm
VGS-2040R 2.0mm x 387mm VGE-2040 2.0mm thick x 387mm
VGS-3012R 3.0mm x 145mm VGE-3012 3.0mm thick x 145mm
VGS-3016R 3.0mm x 160mm VGE-3016 3.0mm thick x 160mm
VGS-3017R 3.0mm x 170mm VGE-3017 3.0mm thick x 170mm
VGS-3019R 3.0mm x 190mm VGE-3019 3.0mm thick x 190mm
VGS-3020R 3.0mm x 220mm VGE-3020 3.0mm thick x 220mm
VGS-3025R 3.0mm x 280mm VGE-3025 3.0mm thick x 280mm
VGS-3040R 3.0mm x 387mm VGE-3040 3.0mm thick x 400mm
VGS-6012R 6.0mm x 145mm VGE-6012 6.0mm thick x 145mm
VGS-6016R 6.0mm x 160mm
VGS-6017R 6.0mm x 170mm
VGS-6019R 6.0mm x 190mm
VGS-6020R 6.0mm x 220mm
VGS-6025R 6.0mm x 280mm
VGS-6040R 6.0mm x 387mm
(2 sides with rounded corners, no bottom)
SILICONE TUBING* BULK GEL WRAP GASKET*
Cat. # Gel Thickness
Cat. # Gel Thickness
ST-0500 0.5mm VGE-05XX 0.5mm thick - yellow
ST-7500 0.75mm VGE-75XX 0.75mm thick - yellow
ST-1000 1.0mm VGE-10XX 1.0mm thick - red
ST-1500 1.5mm VGE-15XX 1.5mm thick - blue
ST-2000 2.0mm VGE-20XX 2.0mm thick - purple
ST-3000 3.0mm VGE-30XX 3.0mm thick - white
*Order by the foot *Order by the foot
GEL WRAP GLASS PLATE SETS, NOTCHED* GLASS PLATE SETS, NOTCHED & BEVELED*
(1/8” back plate with rounded corners) (1/8” back plate with rounded corners)
Cat. # Glass Plate dimensions (w x h)
Cat. # Glass Plate dimensions (w x h)
NGP-125NR 16.5cm x 145mm NGP-125BR 16.5cm x 145mm
NGP-160NR 16.5cm x 160mm NGP-160BR 16.5cm x 160mm
NGP-170NR 16.5cm x 170mm NGP-170BR 16.5cm x 170mm
NGP-190NR 16.5cm x 190mm NGP-190BR 16.5cm x 190mm
NGP-200NR 16.5cm x 220mm NGP-200BR 16.5cm x 220mm
NGP-250NR 16.5cm x 280mm NGP-250BR 16.5cm x 280mm
NGP-400NR 16.5cm x 387mm NGP-400BR 16.5cm x 400mm
* Glass plate ordering options: To order an unnotched plate set and add a “UN” to the end of the catalog # (rubber ears included). Note: Although
glass plates are listed as sets, individual pieces can be ordered. To order a notched only plate, add a “-NO” to the end of the cat. #. To order a back
only (rectangular) plate, add a “-BO” to the end of the cat. #. To order Low fluorescence borosilicate plate sets add “-LFP” to end of cat. #.
www.cbsscientific.com 20 Adjustable Slab Gel Manual 07/13
STANDARD GLASS PLATE SETS, NOTCHED* GLASS PLATE SETS, NOTCHED & BEVELED*
For 2-dimensional gels
Cat. # Glass Plate dimensions (w x h) Cat. # Glass Plate dimensions (w x h)
NGP-125N 16.5cm x 145mm NGP-125B 16.5cm x 145mm
NGP-160N 16.5cm x 160mm NGP-160B 16.5cm x 160mm
NGP-170N 16.5cm x 170mm NGP-170B 16.5cm x 170mm
NGP-190N 16.5cm x 190mm NGP-190B 16.5cm x 190mm
NGP-200N 16.5cm x 220mm NGP-200B 16.5cm x 220mm
NGP-250N 16.5cm x 280mm NGP-250B 16.5cm x 280mm
NGP-400N 16.5cm x 387mm NGP-400B 16.5cm x 387mm
* Glass plate ordering options: To order an unnotched plate set and add a “UN” to the end of the catalog # (rubber ears included). Note: Although
glass plates are listed as sets, individual pieces can be ordered. To order a notched only plate, add a “-NO” to the end of the cat. #. To order a back
only (rectangular) plate, add a “-BO” to the end of the cat. #. To order Low fluorescence borosilicate plate sets add “-LFP” to end of cat. #.
ALUMINUM HEAT DISPERSION PLATES
Cat. # Plate Dimensions
CPA165-200 For use with NGP-200
CPA165-250 For use with NGP-250
CPA165-400 For use with NGP-400
COMBS (Polycarbonate) COMBS (Polycarbonate)
Cat. # Comb dimensions
Cat. # Comb dimensions
VGC-0201 0.2mm x 1 well VGC-0401 0.4mm x 1 well
VGC-0202 0.2mm x 2 well VGC-0402 0.4mm x 2 well
VGC-0203E 0.2mm x 3 equal wells VGC-0403E 0.4mm x 3 equal wells
VGC-0203M 0.2mm x 2 well + middle marker VGC-0403M 0.4mm x 2 well + middle marker
VGC-0204 0.2mm x 4 well VGC-0404 0.4mm x 4 well
VGC-0205 0.2m x 5 well VGC-0405 0.4mm x 5 well
VGC-0206 0.2mm x 6 well VGC-0406 0.4mm x 6 well
VGC-0208 0.2mm x 8 well VGC-0408 0.4mm x 8 well
VGC-0210 0.2mm x 10 well VGC-0410 0.4mm x 10 well
VGC-0212 0.2mm x 12 well VGC-0412 0.4mm x 12 well
VGC-0214* 0.2mm x 14 well VGC-0414* 0.4mm x 14 well
VGC-0215 0.2mm x 15 well VGC-0415 0.4mm x 15 well
VGC-0216 0.2mm x 16 well VGC-0416 0.4mm x 16 well
VGC-0220 0.2mm x 20 well VGC-0420 0.4mm x 20 well
VGC-0229* 0.2mm x 29 well VGC-0429* 0.4mm x 29 well
VGC-0230 0.2mm x 30 well VGC-0430 0.4mm x 30 well
VGC-0501 0.5mm x 1 well
VGC-0502 0.5mm x 2 well
VGC-0503E 0.5mm x 3 equal wells
VGC-0503M 0.5mm x 2 well + middle marker
VGC-0504 0.5mm x 4 well
VGC-0505 0.5mm x 5 well
VGC-0506 0.5mm x 6 well
VGC-0508 0.5mm x 8 well
VGC-0510 0.5mm x 10 well
VGC-0512 0.5mm x 12 well
VGC-0514 * 0.5mm x 14 well
VGC-0515 0.5mm x 15 well
VGC-0516 0.5mm x 16 well
VGC-0520 0.5mm x 20 well
VG
C-0529* 0.5mm x 29 well
VGC-0530 0.5mm x 30 well
SHARKTOOTH COMBS SEQUENCING SPACER SETS (Polycarbonate)
Cat. # Comb dimensions
Cat. # Comb dimensions
SK16.5-0201 0.2mm x 1 well VGS-0216 0.2mm x 160mm
SK16.5-0220 0.2mm x 20 well VGS-0217 0.2mm x 170mm
SK16.5-0226* 0.2mm x 26 well VGS-0219 0.2mm x 190mm
SK16.5-0230 0.2mm x 30 well VGS-0220 0.2mm x 220mm
SK16.5-0242* 0.2mm x 42 well VGS-0225 0.2mm x 280mm
SK16.5-0258* 0.2mm x 58 well VGS-0240 0.2mm x 387mm
SK16.5-0401 0.4mm x 1 well VGS-0416 0.4mm x 160mm
SK16.5-0420 0.4mm x 20 well VGS-0417 0.4mm x 170mm
SK16.5-0426* 0.4mm x 26 well VGS-0419 0.4mm x 190mm
SK16.5-0430 0.4mm x 30 well VGS-0420 0.4mm x 220mm
SK16.5-0442* 0.4mm x 42 well VGS-0425 0.4mm x 280mm
SK16.5-0458* 0.4mm x 58 well VGS-0440 0.4mm x 387mm
SK16.5-0501 0.5mm x 1 well VGS-0516 0.5mm x 160mm
SK16.5-0520 0.5mm x 20 well VGS-0517 0.5mm x 170mm
SK16.5-0526* 0.5mm x 26 well VGS-0519 0.5mm x 190mm
SK16.5-0530 0.5mm x 30 well VGS-0520 0.5mm x 220mm
SK16.5-0542* 0.5mm x 42 well VGS-0525 0.5mm x 280mm
SK16.5-0558* 0.5mm x 58 well VGS-0540 0.5mm x 387mm
*Designed for multi-channel pipettor loading
www.cbsscientific.com 21 Adjustable Slab Gel Manual 07/13
ADDITIONAL VERTICAL GEL ACCESSORIES:
BAR CLAMP SILICONE CORD REPAIR KIT
Cat. # Item Cat. # Item
GPC-1650 Bar clamp for 16.5cm wide units SCK-500 Kit for Sequencing and Slab Gel Units
MULTIPLE GEL CASTING CHAMBERS
Cat. # Item
GCC-404 Multiple Vertical Gel Casting chamber, casts up to 4 gels simultaneously
GCC-410 Multiple Vertical Gel Casting chamber, casts up to 10 gels simultaneously
GEL CASTING STAND
Cat. # Item
GCS-250 Gel Casting Stand, includes stand with leveling base and 4 clamps
RUBBER EARS
Cat. # Item
VFR-1000 Adhesive rubber ears for unnotched glass plate sets
WHITE CLAMPS
Cat. # Item
GPC-0004 Large capacity, multi-task white clamp
GPC-0002 Casting clamp, jaws closed in resting position
GPC-0001 Large white clamp, jaws slightly open in resting position
POWER SUPPLY
Cat. # Item
EPS-300-II Mini Power Supply, with timer, CV or CC, 10-300V, 110V/60Hz, current range:4-500mA, 90 watts
EPS-300-IIV Mini Power Supply, with timer, CV or CC, 10-300V, 220V/50Hz, current range:4-500mA, 90 watts
EPS-3000-III Constant Power Supply, 0-3000 Volt, 110V/60Hz, Series II
EPS-3000-IIV Constant Power Supply, 0-3000 Volt, 220V/50Hz, Series II
NOTES
NOTES
CONTACT US
Telephone:
Local or International
858-755-4959
Toll Free: 800-243-4959
Fax: 858-755-0733
Check out our new and
improved online ordering!
www.cbsscientific.com
E-mail address:
sales@cbssci.com
Mailing address:
C.B.S. Scientific Company
P.O. Box 856
Del Mar, CA 92014
Shipping address:
C.B.S. Scientific Company
10805 Vista Sorrento Pkwy
Suite 100
San Diego, CA 92121
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