Caron 6404-4 User Manual

PO Box 715
Marietta, OH 45750

800-648-3042  740-373-6809
Fax [phone]
www.caronproducts.com
[e-mail]

6 CUFT BENCHTOP

CO2 INCUBATOR

OPERATIONS MANUAL

FOR MODELS
6400-1 and 6400-4
6404-1 and 6404-4

Dear Valued Customer:

Thank you for purchasing CARON Products & Services equipment. We appreciate your
business and look forward to being your preferred supplier of controlled environment
equipment products in the future.

At CARON, we are committed to continuous quality improvement. Our goal is to supply
our customers with highly reliable equipment at a fair price. In order to openly monitor
our performance, we would appreciate your feedback on our products and services.

If you have questions, or any suggestions for improvement based on the installation or
operation of the equipment you have purchased, please contact our service department
at [e-mail] or 740-373-6809.

Thanks again for your business!

6400/6404 Series Operations Manual Page 2 of 54
Rev F 09/18/14

TABLE OF CONTENTS

Section 1 – Warranty…………………………………………………………………..5

Section 2 – Equipment Overview…………………………………………………. 10

Section 3 – Equipment Installation……………………………………………….. 12

Unpacking
Choosing a Location
Preliminary Cleaning
Leveling the Unit
Filling the Humidity Pan
Connecting a CO2 Supply
Connecting a N2 supply
Connecting Electrical Power
Connecting Alarm Contacts

Section 4 – Optional Accessory Installation / Operation……………………...17

Installing Incubators on a Roller Base or Floor Stand
Connecting the CO2 and/or N2 Gas Guard
Connecting Analog Outputs

Section 5 – Equipment Operation………………………………………………… 20

Changing the Temperature Set-point

Changing the CO2 Set-point

Changing the O2 Set-point

Section 6 – Calibration……………………………………………………………… 24

Calibrating the Temperature display

Calibrating the CO2 display

Calibrating the O2 display

Calibrating the Relative Humidity display

Section 7 – Admin Mode……………………………………………………………. 28

Setting the Over/Low Temperature Alarm
Setting the High/Low CO2 Alarm
Setting New CO2 Tank
Setting the High/Low O2 Alarm
Setting the Low RH Alarm
Setting a System Password
Enabling / Disabling Audible Alarm
Setting the Alarm Ring Back Time
Setting the Door Alarm Delay

6400/6404 Series Operations Manual Page 3 of 54
Rev F 09/18/14

Configuring Temp / CO2 / RH / O2 Alarm Relay
Selecting Language
Setting System Date / Time
Resetting Factory Defaults

Section 8 – Datalogging……………………………………………………………..41

Setting the Data Log Time Period
Reviewing Data Logs

Section 9 – Using the Automatic Decon Cycle…………………………………. 43

Section 10 – Alarms…………………………………………………………………. 46

Section 11 – Service Mode…………………………………………………………. 47

Section 12 – Preventative Maintenance…………………………………………. 48

Section 13 – Specifications…………………………………………………………49

Section 14 – Electrical Schematic………………………………………………… 50

Appendix A – Declaration of Conformity………………………………………... 52

6400/6404 Series Operations Manual Page 4 of 54
Rev F 09/18/14

SECTION 1- WARRANTY INFORMATION

CO2 INCUBATOR LIMITED WARRANTY

Please review this section before requesting warranty service. At CARON, one of our primary goals is
to provide customers with high levels of personal service and top quality products, delivered on time,
backed by technical service and supported for the life of the product.

Before contacting us for warranty service, please be aware that there are repairs that are not covered
under warranty.

WARRANTY DEFINED

Caron Products & Services, Inc. (herein after CARON) hereby warrants that equipment manufactured
by CARON is free from defects in materials and workmanship when the equipment is used under
normal operating conditions in accordance with the instructions provided by CARON.

COVERED:

• Parts and labor for a period of two (2) years from date of shipment.

• Any part found defective will be either repaired or replaced at CARON's discretion, free of charge, by
CARON in Marietta, OH. Parts that are replaced will become the property of CARON.

• If CARON factory service personnel determine that the customer's unit requires further service,
dependent of the model involved, CARON may, at its sole discretion, provide a service technician to
correct the problem, or require the return of the equipment to the factory or authorized service depot.

• CARON will have the right to inspect the equipment and determine the repairs or replacement parts
necessary. The customer will be notified, within a reasonable time after inspection, of any costs
incurred that are not covered by this warranty prior to initiation of any such repairs.

NOT COVERED:

• Calibration of control parameters.

• Improper installation; including electrical service, gas and water supply tubing, gas supplies, room
ventilation, unit leveling, facility structural inadequacies or ambient conditions that are out of
specification.

• Cost of express shipment of equipment or parts.

• Any customer modifications of this equipment, or any repairs undertaken without the prior written
consent of CARON, will render this limited warranty void.

• CARON is not responsible for consequential, incidental or special damages; whether shipping
damage or damages that may occur during transfer to the customer’s point of use. When the
equipment is signed for at the customer’s site, ownership is transferred to the customer. Any damage
claims against the shipping company become the responsibility of the customer.

• Repairs necessary because of the equipment being used under other than normal operating
conditions or for other than its intended use.

• Repair due to the customer's failure to follow normal maintenance instructions.

• Parts considered consumable; including: light bulbs, filters, gases, etc.

• Damage from use of improper water quality.

• Damage from chemicals or cleaning agents detrimental to equipment materials.

• Force Majeure or Acts of God.

6400/6404 Series Operations Manual Page 5 of 54
Rev F 09/18/14

This writing is a final and complete integration of the agreement between CARON and the customer.
CARON makes no other warranties, express or implied, of merchantability, fitness for a particular
purpose or otherwise, with respect to the goods sold under this agreement. This warranty cannot be
altered unless CARON agrees to an alteration in writing and expressly stated herein shall be
recognized to vary or modify this contract.

Ohio Law governs this warranty.

6400/6404 Series Operations Manual Page 6 of 54
Rev F 09/18/14

EQUIPMENT INTERNATIONAL LIMITED WARRANTY

Please review this section before requesting warranty service. At CARON, one of our primary goals is
to provide customers with high levels of personal service and top quality products, delivered on time,
backed by technical service and supported for the life of the product.

Before contacting your distributor for warranty service, please be aware that there are repairs
that are not covered under warranty.

WARRANTY DEFINED

Caron Products & Services, Inc. (herein after CARON) hereby warrants that equipment manufactured
by CARON is free from defects in materials and workmanship when the equipment is used under
normal operating conditions in accordance with the instructions provided by CARON.

COVERED:

• Parts for a period of two (2) years from date of shipment.

• Any part found defective will be either repaired or replaced at CARON's or their authorized
representative’s discretion. Parts that are replaced will become the property of CARON.

• If CARON or their authorized representatives determine that the customer's unit requires further
service, CARON or the representative may, at its sole discretion, provide a service technician to correct
the problem, or require the return of the equipment to the an authorized service depot.

• CARON or their authorized representative will have the right to inspect the equipment and determine
the repairs or replacement parts necessary. The customer will be notified, within a reasonable time
after inspection, of any costs incurred that are not covered by this warranty prior to initiation of any
such repairs.

NOT COVERED:

• Calibration of control parameters.

• Improper installation; including electrical service, gas and water supply tubing, gas supplies, room
ventilation, unit leveling, facility structural inadequacies or ambient conditions that are out of
specification.

• Cost of express shipment of equipment or parts.

• Any customer modifications of this equipment, or any repairs undertaken without the prior written
consent of CARON, will render this limited warranty void.

• CARON and their representative are not responsible for consequential, incidental or special
damages; whether shipping damage or damages that may occur during transfer to the customer’s point
of use. When the equipment is signed for at the customer’s site, ownership is transferred to the
customer. Any damage claims against the shipping company become the responsibility of the
customer.

• Repairs necessary because of the equipment being used under other than normal operating
conditions or for other than its intended use.

• Repair due to the customer's failure to follow normal maintenance instructions.

• Parts considered consumable; including: light bulbs, filters, gases, etc.

• Damage from use of improper water quality.

• Damage from chemicals or cleaning agents detrimental to equipment materials.

• Force Majeure or Acts of God.

6400/6404 Series Operations Manual Page 7 of 54
Rev F 09/18/14

This writing is a final and complete integration of the agreement between CARON and the customer.
CARON makes no other warranties, express or implied, of merchantability, fitness for a particular
purpose or otherwise, with respect to the goods sold under this agreement. This warranty cannot be
altered unless CARON agrees to an alteration in writing and expressly stated herein shall be
recognized to vary or modify this contract.

Ohio Law governs this warranty.

Caron Products & Services, Inc.

PO Box 715 · Marietta, OH 45750

740-373-6809

6400/6404 Series Operations Manual Page 8 of 54
Rev F 09/18/14

Warning of hazardous area

Warning of dangerous electric voltage

Earth (ground) protective
conductor

Local government may require proper disposal

Help

Information

i

?

Warning of hot surface

INTERNATIONAL SYMBOLS AND DEFINITIONS

WARNINGS

6400/6404 Series Operations Manual Page 9 of 54
Rev F 09/18/14

Adjustable
Sliding
Shelf

Power
Switch

Control
Panel

Sealed Inner
Glass Door

Heated
Outer Door

CO2 Sample
Port

Humidity Pan

Leveling Foot

Outer Door
Switch

Temp,
Humidity,
and IR CO2
Sensors

Inner Door
Handle

SECTION 2 – EQUIPMENT OVERVIEW

Congratulations! You have just purchased the latest technology in CO2 incubators.
Before using the equipment, familiarize yourself with key components of the product and
thoroughly read this manual.

6400/6404 Series Operations Manual Page 10 of 54
Rev F 09/18/14

SECTION 2 – EQUIPMENT OVERVIEW – CONTINUED

1

2

3

4

7

5

6

8

9

10

11

12

1

Silence Key – Press to Silence audible
alarm

Alarm Indicator – Illuminates on
and off during an alarm condition

2

3

4

5

6

7

8

9

10

12

11

Heat Indicator – Illuminates when
any system heater is on

Inject Indicator – Illuminates when
gas is being injected

Temperature / Humidity Display

CO2 / O2 Display

Message Center – Displays incubator
setpoints and any alarm conditions

Menu Select – Allows user to select
programming menu

Enter – Allows user to enter any
adjusted system variable

Allows user to adjust system
variables up and down

Active Indicator – Illuminates when
incubator is in Decon Cycle

Decon – Press to initiate decon cycle

INCUBATOR CONTROL PANEL

6400/6404 Series Operations Manual Page 11 of 54
Rev F 09/18/14

This product weighs approx. 250 pounds.
Ensure that sufficient resources are available to
safely move the product.

When moving your incubator, do not lift the unit
by the door handle or the outer door as
structural damage could occur.

SECTION 3 – EQUIPMENT INSTALLATION

Unpacking

Your new unit has been thoroughly packaged to avoid shipping damage. However, the
unit should be fully inspected upon arrival before signing for receipt. If the package has
visual damage, make notes accordingly on the freight bill and have it signed by the
delivery company. In the event of concealed damage after the unit is uncrated, keep the
carton and packaging material. Call the shipping company within 7 days of receipt,
request inspection and retain a copy of the inspection report.

For added convenience, your incubator was shipped with shelving and internal
components installed. Remove all foam protective pieces from both outside and inside
the incubator. Remove any tape holding components in position during shipping.

A boxed shipping kit is also included inside the incubator. The shipping kit includes a
user’s manual, electrical cord, gas connection tubing with filters, stacking adaptors with
hardware, and spare incubator hardware.

Choosing a Location

To ensure proper operation, you must locate the unit on a firm level surface, capable of
supporting approximately 250 pounds (500 pounds if stacked). The unit should be
located in an 18°C – 25°C ambient area and where there is no direct airflow from
heating and cooling ducts as well as out of direct sunlight. Allow four to six inches of
clearance on all sides of the product to allow for connections and airflow.

The unit requires a dedicated electrical connection and a regulated pressurized CO2
supply. Choose a location where these facilities are or can be made available.

Your incubators are designed to stack on top of each other to optimize floor space.
Stacking brackets are provided with each incubator to secure them together.

6400/6404 Series Operations Manual Page 12 of 54
Rev F 09/18/14

Failure to properly install the provided stacking
brackets could result in unit tipping.

Chloride containing cleaners can attack and
corrode stainless steel. Cleaners with strong
acids can damage plastic components.

The incubator specifications were developed by testing the product in controlled
environment conditions of 72°F to 77°F degrees with a voltage fluctuation of +/-10%.
Operating the incubator outside of these specifications will affect the incubator’s
performance.

Preliminary Cleaning

Your new incubator was thoroughly cleaned prior to leaving the factory. It is
recommended however, to disinfect all interior surfaces with a general purpose
laboratory cleaning agent prior to using the product. After cleaning, dry all interior
components with a sterile cloth as necessary.

6400/6404 Series Operations Manual Page 13 of 54
Rev F 09/18/14

Before relocating an incubator remove the water
in the humidity pan and transfer any product to
another incubator.

Leveling the Unit

Place a level on the middle shelf of the incubator. The cabinet has built in adjustable
leveling feet designed to level the unit. Adjust the feet appropriately until the unit sits
level left to right and front to back.

Installing Filtered Air Exchange Port Stopper

The Oasis incubator is designed with a filtered air exchange that allows a small amount
of ambient air to be drawn into the incubator to slightly reduce the relative humidity to
approximately 90% and just below saturation. The recirculating fan pulls HEPA filtered
ambient air into the incubator and exhausts the air through a HEPA filtered stopper. The
silicone port stopper HEPA assembly that is supplied in the accessory kit will need to be
installed in the rear access port located on the top left side of the back wall as seen in
the picture below.

If higher humidity levels higher than 90% are required, install the brass plug that is
supplied in the accessory kit into the rear panel of the cabinet labeled Control Leak.
This will disable the filtered air exchange. With the filtered air exchange disabled, the
humidity level may increase above saturation resulting in some condensation developing
on incubator surfaces.

6400/6404 Series Operations Manual Page 14 of 54
Rev F 09/18/14

Use only distilled or deionized water with a
resistivity between 50K-CM and 1M-CM and
a neutral pH. Using water outside this range will
void your warranty.

High concentrations of carbon dioxide can
cause asphyxiation. The use of CO2 monitors
and alarms is recommended for areas where
CO2 can collect.

The CO2 gas supply should be 99.5% pure and
should not contain a siphon tube. Install a 2
stage gas pressure regulator with a maximum
adjustment of 25 psi. Inlet pressure must be
regulated to 15 psi.

Filling the Humidity Pan

To ensure proper operation, distilled or deionized water is required in the humidity pan.
Fill the humidity pan approximately half full of water. Place the pan directly under the
hinged airflow duct sitting on the bottom floor of the incubator to insure optimum
temperature and humidity response. The hinged airduct has “stops” designed into it to
ensure the pan is located in the ideal position. Slide the humidity pan back under the
duct until it hits the “stops”.

Connecting a CO2 supply

The CO2 supply should be 99.5% and not have siphon tubes. Regulate CO2 pressure to
15 psi. CO2 tank regulators are available to purchase through CARON customer
service. Once the cylinder regulator is installed, connect the outlet of the regulator to the
hose barb fitting using the tubing and clamps provided. An inline HEPA filter is provided
to remove any contaminants in the CO2 gas supply. Check the connections closely for
leaks.

6400/6404 Series Operations Manual Page 15 of 54
Rev F 09/18/14

Low levels of oxygen can cause suffocation.
The use of O2 monitors and alarms is
recommended for areas where N2 is used to
suppress oxygen

The N2 gas supply should be 99.5% pure and
should not contain a siphon tube. Install a 2
stage gas pressure regulator with a maximum
adjustment of 25 psi. Inlet pressure must be
regulated to 15 psi.

If the unit is equipped with an optional built in gas guard system, there will be 2 gas
inlets. Each of the inlets should be connected to an individual gas tank as described
above.

Connecting a N2 supply (for suppressed O2 6404 models)

The N2 supply should be 99.5% and not have siphon tubes. Regulate N2 pressure to 15
psi. N2 tank regulators are available to purchase through CARON customer service.
Once the cylinder regulator is installed, connect the outlet of the regulator to the hose
barb fitting using the tubing and clamps provided. An inline HEPA filter is provided to
remove any contaminants in the N2 gas supply. Check the connections closely for leaks.

If the unit is equipped with an optional built in gas guard system, there will be 2 gas
inlets. Each of the inlets should be connected to an individual gas tank as described
above.

6400/6404 Series Operations Manual Page 16 of 54
Rev F 09/18/14

Connect each incubator to a dedicated
grounded circuit. Failure to do so could result in
electrical shock. Ensure that there is
unobstructed access to the main power plug
disconnect.

Connecting Electrical Power

Model 6400-1 requires a 100-130 VAC, 50/60Hz 15A power connection. The power
cord connection is a NEMA 5-15P plug.

Model 6400-4 requires a 220 – 240 VAC, 50/60Hz, 16A power connection. The power
cord connection is a CEE 7/7 plug.

When the required electrical connection is available, plug the provided power cord into
the unit and the electrical outlet.

Connecting Alarm Contacts

A set of dry relay alarm contacts are provided standard with each incubator. They are
located on the back of the incubator. The terminals on the relay contact allow for a NO
(normally open) output, a NC (normally closed) and COM (common) connection.

In the event of an alarm condition or power failure, the NO contact will close and the NC
contact will open. Once the alarm is cleared, the contacts will return to their normal
position.

6400/6404 Series Operations Manual Page 17 of 54
Rev F 09/18/14