Caron 6044 User Manual

PO Box 715
Marietta, OH 45750

800-648-3042  740-373-6809
Fax [phone]
www.caronproducts.com
[e-mail]

REACH-IN CO2 INCUBATOR

OPERATIONS MANUAL

FOR MODELS

6024, 6026, 6044, 6046

Dear Valued Customer:
Thank you for purchasing CARON Products & Services equipment. We appreciate your

business and look forward to being your preferred supplier of controlled environment
equipment products in the future.

At CARON, we are committed to continuous quality improvement. Our goal is to supply
our customers with highly reliable equipment at a fair price. In order to openly monitor
our performance, we would appreciate your feedback on our products and services.

If you have questions, or any suggestions for improvement based on the installation or
operation of the equipment you have purchased, please contact our service department
at [e-mail] or 740-373-6809.

Thanks again for your business!

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 2 of 56

TABLE OF CONTENTS

Section 1 – Warranty ........................................................................................ 5

Section 2 – Equipment Overview .................................................................... 10

Section 3 – Equipment Installation ................................................................. 12

Unpacking
Choosing a Location
Preliminary Cleaning
Installing the Port Stopper
Installing the Shelves
Leveling the Unit
Connecting the Drain Line
Connecting the Water Supply
Connecting Electrical Power

Section 4 – Optional Accessory Installation .................................................. 17

Connecting Alarm Contacts (ALRM301)
Installing Carboy water system (BOTL301)
Installing Cell Roller Ramp & Support Kit (CELR301)
Connecting Communications or Analog Outputs (DLUX303)
Installing Drain Water Pump (PUMP301)
Installing Side Mounted Recorders (RCDR305/RCDR306)

Section 5 – Operation ................................................................ ...................... 23

Changing the Temperature Set-point

Changing the Humidity Set-point
Changing the CO2 Set-point
Running a Decontamination cycle

Section 6 – Optional Accessory Operation .................................................... 28

Carboy water system (BOTL301)
Delux controller system (DLUX303)
Built in Gas Guard System (GASG301)
Interior Electrical Outlet (OUTL301/OUTL302)
Built in Temp or Temp/Rh 6” Pen Recorders (RCDR301/RCDR302)
Built in Temp or Temp/Rh 10” Thermal Recorders (RCDR303/RCDR304)
Side Mounted Temp/Rh 10” Pen Recorder (RCDR305/RCDR306)
Multi Tier Shaker Support System (SHKR301/SHKR302/SHKR303)

Section 7 – Calibration .................................................................................... 42

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Calibrating the Temperature
Calibrating the Humidity
Calibrating the CO2

Section 8 – Alarms .......................................................................................... 45

Alarm System Overview
Changing Alarm Set-points

Section 9 – Preventative Maintenance .......................................................... 46

Section 10 – Specifications ............................................................................ 47

Section 11 – Electrical Schematics ............................................................... 48

Section 12 – Troubleshooting ......................................................................... 51

Section 13 – Spare Replacement Parts ......................................................... 52

Section 14 – Advanced Users Section .......................................................... 55

Unlocking the Controllers

Locking the Controllers

Appendix A – Declaration of Conformity ....................................................... 56

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 4 of 56

SECTION 1- WARRANTY INFORMATION

CO2 INCUBATOR LIMITED WARRANTY

Please review this section before requesting warranty service. At CARON, one of our primary goals is
to provide customers with high levels of personal service and top quality products, delivered on time,
backed by technical service and supported for the life of the product.

Before contacting us for warranty service, please be aware that there are repairs that are not
covered under warranty.

WARRANTY DEFINED

Caron Products & Services, Inc. (herein after CARON) hereby warrants that equipment manufactured
by CARON is free from defects in materials and workmanship when the equipment is used under
normal operating conditions in accordance with the instructions provided by CARON.

COVERED:

 Parts and labor for a period of two (2) years from date of shipment.
 Any part found defective will be either repaired or replaced at CARON's discretion, free of charge,

by CARON in Marietta, OH. Parts that are replaced will become the property of CARON.

 If CARON factory service personnel determine that the customer's unit requires further service,

dependent of the model involved, CARON may, at its sole discretion, provide a service technician to
correct the problem, or require the return of the equipment to the factory or authorized service
depot.

 CARON will have the right to inspect the equipment and determine the repairs or replacement parts

necessary. The customer will be notified, within a reasonable time after inspection, of any costs
incurred that are not covered by this warranty prior to initiation of any such repairs.

NOT COVERED:

 Calibration of control parameters.
 Improper installation; including electrical service, gas and water supply tubing, gas supplies, room

ventilation, unit leveling, facility structural inadequacies or ambient conditions that are out of
specification.

 Cost of express shipment of equipment or parts.
 Any customer modifications of this equipment, or any repairs undertaken without the prior written

consent of CARON, will render this limited warranty void.

 CARON is not responsible for consequential, incidental or special damages; whether shipping

damage or damages that may occur during transfer to the customer’s point of use. When the

equipment is signed for at the customer’s site, ownership is transferred to the customer. Any

damage claims against the shipping company become the responsibility of the customer.

 Repairs necessary because of the equipment being used under other than normal operating

conditions or for other than its intended use.

 Repair due to the customer's failure to follow normal maintenance instructions.
 Parts considered consumable; including: light bulbs, filters, gases, etc.
 Damage from use of improper water quality.
 Damage from chemicals or cleaning agents detrimental to equipment materials.
 Force Majeure or Acts of God.

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 5 of 56

This writing is a final and complete integration of the agreement between CARON and the customer.
CARON makes no other warranties, express or implied, of merchantability, fitness for a particular
purpose or otherwise, with respect to the goods sold under this agreement. This warranty cannot be
altered unless CARON agrees to an alteration in writing and expressly stated herein shall be
recognized to vary or modify this contract.

Ohio Law governs this warranty.

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 6 of 56

EQUIPMENT INTERNATIONAL LIMITED WARRANTY

Please review this section before requesting warranty service. At CARON, one of our primary goals is
to provide customers with high levels of personal service and top quality products, delivered on time,
backed by technical service and supported for the life of the product.

Before contacting your distributor for warranty service, please be aware that there are repairs
that are not covered under warranty.

WARRANTY DEFINED

Caron Products & Services, Inc. (herein after CARON) hereby warrants that equipment manufactured
by CARON is free from defects in materials and workmanship when the equipment is used under
normal operating conditions in accordance with the instructions provided by CARON.

COVERED:

 Parts for a period of two (2) years from date of shipment.
 Any part found defective will be either repaired or replaced at CARON's or their authorized

representative’s discretion. Parts that are replaced will become the property of CARON.

 If CARON or their authorized representatives determine that the customer's unit requires further

service, CARON or the representative may, at its sole discretion, provide a service technician to
correct the problem, or require the return of the equipment to the an authorized service depot.

 CARON or their authorized representative will have the right to inspect the equipment and

determine the repairs or replacement parts necessary. The customer will be notified, within a
reasonable time after inspection, of any costs incurred that are not covered by this warranty prior to
initiation of any such repairs.

NOT COVERED:

 Calibration of control parameters.
 Improper installation; including electrical service, gas and water supply tubing, gas supplies, room

ventilation, unit leveling, facility structural inadequacies or ambient conditions that are out of
specification.

 Cost of express shipment of equipment or parts.
 Any customer modifications of this equipment, or any repairs undertaken without the prior written

consent of CARON, will render this limited warranty void.

 CARON and their representative are not responsible for consequential, incidental or special

damages; whether shipping damage or damages that may occur during transfer to the customer’s

point of use. When the equipment is signed for at the customer’s site, ownership is transferred to

the customer. Any damage claims against the shipping company become the responsibility of the
customer.

 Repairs necessary because of the equipment being used under other than normal operating

conditions or for other than its intended use.

 Repair due to the customer's failure to follow normal maintenance instructions.
 Parts considered consumable; including: light bulbs, filters, gases, etc.
 Damage from use of improper water quality.
 Damage from chemicals or cleaning agents detrimental to equipment materials.
 Force Majeure or Acts of God.

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 7 of 56

This writing is a final and complete integration of the agreement between CARON and the customer.
CARON makes no other warranties, express or implied, of merchantability, fitness for a particular
purpose or otherwise, with respect to the goods sold under this agreement. This warranty cannot be
altered unless CARON agrees to an alteration in writing and expressly stated herein shall be
recognized to vary or modify this contract.

Ohio Law governs this warranty.

Caron Products & Services, Inc.

PO Box 715 · Marietta, OH 45750

740-373-6809

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 8 of 56

Warning of hazardous area

Warning of dangerous electric voltage

Earth (ground) protective
conductor

Local government may require proper disposal

Help

Information

i

?

Warning of hot surface

INTERNATIONAL SYMBOLS AND DEFINITIONS

WARNINGS

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 9 of 56

Left Side
Access Port

Power
Switch

Control
Panel

Adjustable
Sliding Shelf

Right Side
Access Port

RTD
Temperature
Sensor

Infrared CO2
Sensor

CO2 Sample
Port

Heated Triple Pane
Outer Glass Door

Outer Door Drip
Trough

Humidity
Sensor

Replaceable air
intake filter
(refrigerated
models)

SECTION 2 – EQUIPMENT OVERVIEW

Congratulations! You have just purchased the latest technology in CO2 incubators.
Before using the equipment, familiarize yourself with key components of the product and
thoroughly read this manual.

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 10 of 56

SECTION 2 – EQUIPMENT OVERVIEW – CONTINUED

Alarm Indicator
LED

Temperature
Controller

Humidity
Controller

CO2
Controller

CO2 Disable

Humidity
Disable

Alarm
Silence

Start Decon
Cycle Switch

INCUBATOR CONTROL PANEL

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 11 of 56

SECTION 3 -- INSTALLATION

This product weighs in excess of 500 pounds.
Ensure that sufficient resources are available to
safely move the product.

Unpacking

Your new unit has been thoroughly packaged to avoid shipping damage. However, the
unit should be fully inspected upon arrival before signing for receipt. If the package has
visual damage, notes should be made on the freight bill and signed by the delivery
company. In the event of concealed damage after the unit is uncrated, keep the carton
and packaging material. Call the shipping company within 7 days of receipt, request
inspection and retain a copy of the inspection report.

Caron provides full on-site installation services for all models. Our installation services
guarantees the proper set-up and startup of all equipment. Please contact the Service
Department at 740-373-6809 or [e-mail] for details.

Choosing a Location

To ensure proper operation, the unit must be located on a firm level surface, capable of
supporting approximately 800 pounds. The unit should be located in an 18°C – 25°C
ambient area and where there is no direct airflow from heating and cooling ducts as well
as out of direct sunlight. Allow four inches of clearance on all sides of the product to
allow for connections and airflow.

The unit requires a dedicated electrical connection, a quality water source, and a floor
drain. Choose a location where these facilities are or can be made available. If a water
source, or a drain is not available, contact CARON customer service and ask about our
CRYS102 product line or click this web link for information on the product:

http://www.caronproducts.com/65

Preliminary Cleaning

Your new incubator was thoroughly cleaned prior to leaving the factory. It is
recommended however, to disinfect all interior surfaces with a general purpose
laboratory cleaning agent prior to using the product. After cleaning, dry all interior
components with a sterile cloth as necessary.

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 12 of 56

Installing the Port Stoppers

Each shelf is capable of supporting a uniformly distributed
load of 50 pounds. The maximum chamber capacity is
500 pounds (stationary). Optional shelving is available for
supporting heavy loads such as shakers or cell rollers.
Chamber should be empty when being moved.

Do not pull multiple loaded shelves out simultaneously or
the chamber may tip.

The unit has an access port built into each side of the cabinet. The ports are designed
to allow customer access for equipment validation or for installation of other equipment
inside the chamber. These ports should be sealed with the provided rubber stoppers to
allow the incubator to function properly. Install the stoppers provided in the port on each
side of the unit.

Installing the Shelves

Each incubator includes four (6024, 6026) or five (6044, 6046) perforated stainless steel
shelves. Each shelf requires two shelf channels for installation. Prior to installation,
take time to consider what the size of the product being placed in the incubator will be
and set the shelf spacing accordingly. Additional shelving can be purchased through
CARON customer service if necessary.

To install the shelf channels slide on end of the shelf track into the appropriate shelf
location in the rear plenum. Hook the front of the shelf track into the corresponding
location on either the right or left side front shelf support pilaster. Repeat the process on
the opposite side of the incubator.

If your incubator was purchased with an optional shelving support system such as a
shaker support system or cell roller support system please see the accessories section
of the manual for details regarding installation.

Leveling the Unit

Place a level on the middle shelf of the incubator. Adjust the feet until the unit sits level
left to right and front to back. Models 6024 & 6026 have the leveling feet built into the
caster. Even if the unit is level without adjustment, the leveling feet should still be
lowered to avoid the cabinet moving while opening and closing the outer door & prevent
a flat spot from forming on the casters.

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Connecting the Drain

When using a pressurized water source, failure
to connect the unit to a drain could result in
facility flooding.

The drain line relies on gravity to remove water
from the chamber. The drain line must remain
below the chamber to drain properly. Kinks or
elevations in the drain line above the cabinet
drain will not allow the chamber to drain.

The incubators control humidity by injecting water only as needed. This eliminates
standing water which is a primary source of contamination and corrosion. There are
several ways to take advantage of this feature. The simplest method is to connect the
drain fitting and tubing supplied with the incubator to a local floor drain. During
operation, any water that is not evaporated inside the cabinet will be sent to the cabinet
drain to avoid standing water, minimizing the risk of contamination and corrosion.

If a floor drain is not available, CARON offers a water recirculation system accessory
(CRSY102) that acts as both a water supply and a drain for humidified incubators. This
system continuously recycles any excess water not needed by the incubator, filters and
conditions it, and reuses it to control humidity.

If neither a floor drain, nor a CRSY102 are available, another option is a carboy for a
water source and a plug in the interior incubator drain. While this solution is not
recommended due to the creation of standing water in the bottom of the incubator, it will
allow the incubator to control humidity, with some limitations, while not requiring a drain.
BOTL101 is a carboy accessory that can be purchased through CARON. A drain plug
is provided in the shipping kit for each incubator. When the interior drain is plugged, at
humidity levels above 80%, water can condense on the door, filling up the drain pan
located below the door.

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 14 of 56

Use only distilled or deionized water with a
resistivity between 50K-CM and 1M-CM and
a pH of greater than 6.5. Using water outside
this range will void your warranty.

Do not use water that contains chloramines.
Chloramines can damage internal rubber
gaskets resulting in leaks.

High concentrations of carbon dioxide can
cause asphyxiation. The use of CO2 monitors
and alarms is recommended for areas where
CO2 can collect.

The CO2 gas supply should be 99.5% pure and
should not contain a siphon tube. Gas pressure
to the unit must be regulated to 15-20PSI.
Failure to do so could cause tubing to burst.

Connecting the Water Supply

To ensure proper operation, distilled or deionized water is required as a supply on units
that have humidity control. If these water sources are not available contact CARON
customer service.

A water inlet fitting on the back of the unit and ¼” black tubing are provided to connect
the water supply to the chamber. Connect an appropriate water supply to the fitting.
Incoming line pressure should be regulated to not exceed 80 psi.

If a Condensate Recirculator water recycling system was purchased as a water supply,
refer to its user’s manual for proper installation of the water supply.

Connecting a CO2 supply

The CO2 supply should be 99.5% and not have siphon tubes. CO2 pressure should be
regulated to 15-20 psi. CO2 tank regulators can be purchased through CARON
customer service. Once the cylinder regulator is installed, connect the outlet of the

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 15 of 56

Connect each incubator to a grounded circuit.
Failure to do so could result in electrical shock.

regulator to the hose barb fitting using the tubing and clamps provided. An inline HEPA

Model #

Power Requirements

Plug Connection

-1

115V, 60Hz, 16A FLA

NEMA 5-20

-2

230V, 60Hz, 10A FLA

NEMA 6-15

-3

230V, 50Hz, 8A FLA

CEE 7/7

filter is provided to remove any contaminants in the CO2 gas supply. Check the
connections closely for leaks.

If the unit is equipped with a built in gas guard system, there will be 2 gas inlets. Each of
the inlets should be connected to an individual gas tank as described above.

Connecting Electrical Power

The unit requires a dedicated electrical outlet. See table below for model specific power
required and connection.

When the required electrical connection is available, plug the provided power cord into
the unit and the electrical outlet.

6024 & 6044 Series Operations Manual Rev F 02/14/12 Page 16 of 56

SECTION 4 – OPTIONAL ACCESSORY INSTALLATION

Terminal Connection

Unit off

Normal

Alarm

N/O to C

Closed

Open

Closed

N/C to C

Open

Close

Open

Connecting Alarm Contacts (ALRM301)

With the purchase of ALRM301, a set of terminals on the rear of the unit is provided to
monitor for temperature, humidity, or CO2 alarms. With the purchase of DLUX303, a set
of terminals is provided to connect RS485 communications and analog outputs.

With the alarm contacts, the terminals provided allow for a NO (normally open) output, a
NC (normally closed) and COM (common) connection. In the event of an alarm
condition or power failure, the NO contact will close, and the NC contact will open. Once
the alarm is cleared, the contacts return to their normal conditions. Insert the
appropriate wire into the terminal and tighten down the screw terminal on top of the
connector.

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