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H-C Mixer
Operating Instructions
Model 270600
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Table of Contents
1. Safety ..................................................................................................................... 3
2. General Information .............................................................................................. 4
3. Getting Started ...................................................................................................... 5
3.1 Unpacking ............................................................................................................. 5
4. Operation ............................................................................................................... 6
4.1 Operation Controls (Fi gure 1) .............................................................................. 6
4.2 Mounting exchangeable Thermal Mixer Blocks. ................................................ 7
4.3 Mode Selection ..................................................................................................... 7
4.4 Mode 1 Operation ................................................................................................. 8
4.5 Mode 2 Operation ................................................................................................. 9
4.6 Mode 3 Operation ............................................................................................... 11
5. Specifications ...................................................................................................... 13
6. Warranty and Service ......................................................................................... 14
6.1 Warranty ............................................................................................................. 14
6.2 Service ................................................................................................................. 14
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1. Safety
The following symbols mean:-
Caution: Read these operating instructions fully before use and pay
particular attention to sections containing this symbol
Caution: Surfaces can become hot during use.
Always observe the following safety precautions
Use only as specified by the operating instructions, or the intrinsic protection may be impaired.
After transport or storage in humid conditions, dry out the unit before connecting it to the supply
voltage.
As the unit is producing shaking or rotational movement, be aware of the surface that the unit will be
placed upon.
Connect only to a power supply with a voltage corresponding to that on the serial number label.
Ensure that the main switch and isolating device (power supply connector) are easily accessible during
use.
Before moving, disconnect at the power supply socket.
If liquid is spilled inside the unit, disconnect it from the power supply and have it checked by a
competent perso n.
It is the user's responsibility to carry out appropriate decontamination if hazardous material is spilt
on or inside the equipment.
Before using any cleaning or decontamination method except those recommended by the
manufacturer, user should check with the manufacturer that the proposed method will not damage
the equipment.
Clean the unit only with a damp cloth; do not use chemical cleaning agents.
Place unit on a solid, level work surface or laboratory bench.
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2. General Information
The H-C Mixer is designed for shaking and controlling a set temperature in a range from 18ºC
below ambient to 99ºC in 0.5, 1.5, 2.0 ml micr o test tubes and Standard/Deep well micro-titre
plates and PCR tubes and plates.
This device can be used in:
• Immuno-chemistry for sample shaking at a constant temperature
• Biochemistr y for enzyme analysis
• Other general research laboratory applications
In addition to the controlled temperature mixing function of the H-C M ixer, the unit al so has the
functionality of a mix-pause function. In this function, the temperature is controlled and the unit
can be programmed to mix for an established period of time and then paused for a set period of
time. The mix-pause function can be programmed by the end user and cycled for a predetermined period of time.
The H-C Mixer can also be programmed to control up to two timed set temperature modes while
mixing the sample. The temperature, mixing speed and program duration are independently
controlled for greater versatility in the laboratory.
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3. Getting Started
3.1 Unpacking Remove packing materials carefully, and retain for future shipment or storage of the unit.
The Boekel Scientific H-C Mixer includes:
• Thermal-mixer ………………………………...................................1 piece
• External Power Supply ................................................................1 piece
• Accessory Block for 20 x 1.5/2.0 microtubes ……… ……………..1 piece
• Operating Instructions………………………………………….........1 copy
3.2 Instrument Placement
Place the H-C Mixer on a solid level surface so that there is at least 10 cm of clearance from
adjacent walls and devices and that the ventilation slots on the side of the unit are free from
obstruction.
Connect the H-C M i xe r to the AC/DC adapter and the adapter to the main power supply and
the power cord to power supply outlet.
Switch the unit to the on position utilizing the power on/off switch located on the rear left side
of the instrument.
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4. Operation
(1) (2) (3) (4) (5) (6) (7) (8)
(9) (10) (11) (12) (13) (14)
4.1 Operation Controls (Figure 1)
(1) Mode Display
(2) Set Temperature Display (top)/ Actual Temperature Display(bottom)
(3) Set Time Display (top)/ Actual Time Remaining Display (bottom)
(4) Mode 1 Program button
(5) Mode 2 Program button
(6) Mode 3 Program button
(7) Function Toggle arrow
(8) Save program Button
(9) Set RPM Display (top)/ Actual RPM Display (bottom)
(10) RUN/PAUSE/STOP/End Function Indicator
(11) Temperature Adjustment buttons
(12) RPM Adjustment buttons
(13) Time Adjustment buttons
(14) Start/Stop/Reset button
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4.2 Mounting exchangeable H-C Mixer Blocks.
The H-C Mixer includes a Therm al block for 20 x 1.5/2.0 ml Tubes. The unit can also be
fitted with three addition Thermal Blocks that can accommodate 0.5 ml tubes, 96-well PCR
plates and Standard and Deep Well Micro Titre plates. To chan ge and mount the accessory
plates, turn the unit off and disconnect it from the powe r sour ce. Remove the 4 chrome
thumb screws and carefully remove the thermal block from the unit. Sele ct the accessory
block to be used and align the block on the top of the thermal Mixer Base. The four setscrew holes will align and the block will seat nicely on the top of the unit. Replace the f our
chrome thumb screws into the accessory block being ca ref ul not to over-tighten the screws.
Reconnect the power supply and turn the unit on for operation.
The following Accessories are available:
Boekel Catalog # Description
270690 Thermal Bock for 20 x 1.5/2.0 ml tubes (Supplied with unit)
270691 Thermal Block for 20 x 0.5 ml tubes
270692 Thermal Block for 96-well P C R pla t e
270693 Thermal Block for Standard and deep well Micro Titre P late s
4.3 Mode Selection There are three programmable modes capable of storin g up to ten programmable settings
per mode. To select a mode, press the appropriate Mode Butto n. To select a specific
program in a Mode, press the Mode button until the Mode display reads the selected
program.
Mode 1- This mode is used for set temperature and mixing control for a
set/unlimited period of time.
Mode 2- This mode is used for a set temperature control with a mix/pause function
that can control the length of time that the samples are mixed and paused
for a pre-determined period of time.
Mode 3- This mode is used to control a set temperature and mixing speed
requirement for a pre-determined period of time then change to a second
set temperature and mixing speed req uir e me nt for a second pre-
determined period of time.
Note: To change between modes during use, the Function Indicator must be in the
“STOP” condition. If a program function is in-process, press and hold the “Start/Stop”
button until an audible beep is heard and the display reads “STOP”.
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4.4 Mode 1 Operation
Mode 1 is used for set temperature and mixing cont rol for a set/unlimited period of time and
can be programmed for up to 10 programmable user functions.
The mode display will show the Mode and the program setting. For example, the display
above shows 1-0 for the mode setting, indicating Mode 1 program 0. For this mode
operation, use the Temper at ure adj ustment buttons, RPM adjustment buttons and Ti me
adjustment buttons to set the Temperature, RPM, and Mix Time.
The temperature can be set from 2°C to 99°C or the Temperature control function can be
turned off by holding down the Temperature adjustment arrow until the Temperature display
reads “off” and audible beep is heard.
Note: During cooling operation, increased fan velocity may be observed.
The timer can also be set to count up by holding down the Time adjustment arrow until the
time display reads “off” and audible beep is heard.
To save the set parameters to the Mode, press and hold the Save button until an Audible
beep is heard.
Press the Start/Stop button to start the operation. As in the figure above, “RUN” will appear
in the function indicator area showing that an operation is in process.
Pressing the Start/Stop button once will “PAUSE” the operation. Pressing the Start/Stop
button again will resume the operation. Upon completion of the operation an audible beep will
sound and the Function indicator will read “END”.
Note: Upon the completion of an operation, the unit will stop mixing. The heat/cool
function will continue to operate at the last set temperature.
To reset a program to the starting point or change function parameters during the operation
of a program, first pause the cycle by pressing the Start/Stop button. Once paused, the
mode cycle will need to be reset by pressing and holding the Start/Stop button for
approximately 3 seconds until an audible beep is heard and the function indicator reads
“STOP”. At this point you can change function parameters or start a new program cycle.
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Following is an example of Mode 1 Program setting to run at 50°C at 400 RPM for 3h:45m:
1. Use Temp arrow buttons to select 50⁰C.
2. Use Mix arrow buttons to select 400RPM.
3. Use Time arrow buttons to select 3h:45m
4. Depress Save Key if you wish to save the program.
5. Depress the “Start/Stop button to start program.
6. Unit will begin to run. Timer will begin to count down.
7. Upon completion of program the unit will stop and the display will indicate “End”
4.5 Mode 2 Operation
Programmable Mix or Pause time
Mix/Pause Indicator Symbol
Mode 2 is used for a set temperature control with a mix/pause function that can control the
length of time that the samples are mixed and paused for a pre-determined period of time
and can be programmed for up to 10 programm abl e user func tions.
The mode display will show the Mode and the program setting. For example, the display
above shows 2-0 for the mode setting, indicating Mode 2 program 0. For this mode
operation, Use the temperature adjustment buttons, Rpm adjustment buttons and Time
adjustment buttons to set the Temperature, RPM, and total program Time.
The temperature can be set from 2°C to 99°C or the Temperature control function can be
turned off by holding down the Temperature adjustment arrow until the Temper ature display
reads “off” and audible beep is heard.
Note: During cooling operation, increased fan velocity may be observed.
Set the total program time by using the Time adjustment buttons. The total progr am time i s
the time that the program will run while cycling between the mix/pause time cycles.
To set the time that the unit will mix, press the function Toggle Arrow once and the mix
indicator “╤” will blink indicating the ability to set the mix time for the function. Set the mix
time by using the Time adjustment buttons. Press the Toggle arrow again to toggle to the
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pause set time function. The Pause indicator “╧” will blink indicating the ability to set the
pause time of the function. Set the pause time by using the time adjustment buttons.
To save the set parameters to the Mode, press and hold the Save button until an Audible
beep is heard.
Press the Start/Stop button to start the operation. As in the figure above, “RUN” will appear
in the function indicator area showing that an operation is in process.
The mix/pause cycle will continue until the total set time for the complete cycle has elapsed.
Pressing the Start/Stop button once will “PAUSE” the operation. Pressing the Start/Stop
button again will resume the operation. Upon completion of the operation an audible beep will
sound and the Function indicator will read “END”.
Note: Upon the completion of an operation, the unit will stop mixing. The heat/cool
function will continue to operate at the last set temperature.
To reset a program to the starting point or change function parameters during the operation
of a program, first pause the cycle by pressing the Start/Stop button. Once paused, the
mode cycle will need to be reset by pressing and holding the Start/Stop button for
approximately 3 seconds until an audible beep is heard and the function indicator reads
“STOP”. At this point you can change function parameters or start a new program cycle.
Following is an example of Mode 2 Program setting to run at 60°C at 500 RPM for a total run
time of 2h:30m with the unit mixing for 3 minutes and pausing for 1 minute:
1. Use Temp arrow buttons to select 60⁰C.
2. Use Mix arrow buttons to select 500RPM.
3. Use Time arrow buttons to select 2h:30m.
4. Depress “ →” button. “ ╤ “will blink on the display indicating the “cycle run time”. Use
Time arrow buttons to select a cycle run time of 0h:03m.
5. Depress “→” button again. “ ” will blink on the display indicating the “cycle pause
time”. Use Time arrow buttons to select a cycle pause time of 0h:01m.
6. Depress “→” button to return to the home mode screen.
7. Depress the Save button if you wish to save the program.
8. Depress the “Start/Stop” button to start program.
9. Unit will begin to alternate between running for 3min, and pausing for 1min, this will
continue for a total of 2h:30m. During the pause function, the unit will maintain the set
temperature while the mixing has paused.
10. Upon completion of program the unit will stop and display will indicate “End”.
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4.6 Mode 3 Operation
Fig. 2
Program 1 Setting indicator
Fig. 3
Program 2 Setting Indicator
Mode 3 is used to control a set temperature and mixing speed requirement for a pre-
determined period of time then change to a second set temperature and mixing speed
requirement for a second pre-determined period of time and can be programmed for up to 10
programmable user fu ncti ons .
The mode display will show the Mode and the program setting. For example, the display
above shows 3-0 for the mode setting, indicating Mode 3 program 0. For this mode
operation, Use the temperature adjustment buttons, Rpm adjustment buttons and Time
adjustment buttons to set the Temperature, RPM, and initial timed temperature cycle. The
Setting indicator will show one solid block indicating that you are programming the first timed
setting of the program. See Fig. 2.
To set the second program setting of the cycle, press the Toggle Arrow once until two Solid
blocks appear, see Fig. 3. For the second program function, set the temperature , RPM, and
time for the second program setting. Press and hold the Save button until an audible beep is
heard to save the program.
Note: During cooling operation, increased fan velocity may be observed.
Press the Start/Stop button to start the operation, “RUN” will appear in the function indicator
area showing that an operation is in process. A single solid block in the Program Setting
indicator shows that the program is in the first phase of the program. Two solid blocks in the
Program Setting indicator shows that the program is in the final phase of the program.
Pressing the Start/Stop button once will “PAUSE” the operation. Pressing the Start/Stop
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button again will resume the operation. Upon completion of the operation an audible beep will
sound and the Function indicator will read “END”.
Note: Upon the completion of an operation, the unit will stop mixing. The heat/cool
function will continue to operate at the last set temperature.
To reset a program to the starting point or change function parameters during the operation
of a program, first pause the cycle by pressing the Start/Stop button. Once paused, the
mode cycle will need to be reset by pressing and holding the Start/Stop button for
approximately 3 seconds until an audible beep is heard and the function indicator reads
“STOP”. At this point you can change function parameters or start a new program cycle.
Following is an example of a Mode 3 Program. The 1st program step will heat at 90°C at
1200 RPM for a run time of 1h:30m, and a 2nd program step will cool at 25°C at 250 RPM for
a run time of 4h:00m:
1. When you enter Mode 3 a solid block indicating the 1st program step , will be
displayed in the upper left corner of the display as indicated in Fig. 2.
2. Use Temp arrow buttons to select 90⁰C.
3. Use Mix arrow buttons to select 1200RPM.
4. Use Time arrow buttons to select 1h:30m.
5. Depress the “→” button; a 2nd solid block indicating the 2nd program step will appear on
the display as indicated in Fig. 3.
6. Use Temp arrow buttons to select 25⁰C.
7. Use Mix arrow buttons to select 250RPM.
8. Use Time arrows to select 4h:00m.
9. Depress the “Save” button if you wish to save the program.
10. Depress “Start/Stop” button to start the cycle.
11. Unit will now run for 1h:30m at 90⁰C and 1200RPM, at the conclusion of 1h:30m the unit
will then run for 4h:00m at 25⁰C and 250RPM.
12. The unit will stop and display “end” at the conclusion of both steps.
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5. Specifications
The Boekel Scientific H-C Mixer provides:
•Adjustable speed offers gentle or vigorous mixing action
•Precision temperature control from 18 ºC below ambient to 99 ºC
•Multiple Thermal block configurations for 0.5, 1.5/2.0 MCT, PCR plats and Standard/Deep
well Micro Titre Plates
•Programmable control for standard operation, Mix/Pause function and heat/cool
programmable Mode with up to 10 programmable settings per mode.
•Temperature control range…………………………………………...…18 ºC belo w ambient to 99 ºC
•Orbit ..........................................................................................................................................2 mm
•Independent timer with sound signal ………...........................................................1m – 99h:59m
•Speed range ………………………………….............................................................250 - 1400 RPM
•Rotational speed setting ……….....………...................................................................10 rpm
•Heat-up Time.............................................................................................................5 ºC per Minute
•Cool-down Time ……….2-3 ºC between 99 ºC and Ambient, 0.5-1 ºC between ambient and 2 ºC
•Display ……………..……………….………........................................................................16x4, LCD
•Block loading capacity ………………………..
•Nominal voltage ………………….….………….........................19V, 6.32 A via 100/240 AC adapter
•Dimensions …………………….............................................................175 m m x 185 mm x 90 mm
•Weight with power supply ..………...……............................................................................7.25 kg
.......................................................20 x 1.5/2.0 ml tubes
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6. Warranty and Service
6.1 Warranty
When used in laboratory conditions and according to these operating instructions
Boekel warrants this product to be free of defective parts, materials and workmanship
for a period of two years from the date of shipment. The liability of Boeke l Scientific for
any defective equipment during the warranty period shall be limited to the repair of
6.2 Service
defective equipment or replacement thereof without charge for parts or labor.
A Boekel Scien t if ic Re t urned Material Authorization (RMA) number provided by Boekel
Scientific is required before any Boekel products are returned for any reason. Contact
Boekel Customer Service at 1-800-336-6929 Extension 5. A Decontamination Certificate
must be completed, signed by the user, and returned to Boekel Scientific prior to
receiving the RMA number. Please be sure to mark the outside of the returned goods
package with this RMA number to ensure prompt handling.
Boekel Scientific
855 Pennsylvania Blvd.
Feasterville, PA 19053
PHONE: (215) 396-8200 or (800) 336-6929
FAX: (215) 396-8264
Email: Boekel-info@boekelsci.com
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