Boekel Scientific 136400-2 User Manual

INSTRUCTIONS FOR INCUBATOR - SHAKER II

MODELS 136400, 136400S & 136400-2

(PLEASE READ THESE INSTRUCTIONS COMPLETELY BEFORE OPER ATING)

PRODUCT INFORMATION

The Boekel Shake ‘N’ Bake ( Incubator Shaker II) is de­signed for scientists that pr efer to use heat sealed bags
and Tupperware
bridizing and pre-hybridizing membranes in Southern
and Northern blotting techniques. The Boek el Shake ‘N’
Bake uses a microprocessor to control incubation tem­perature. The unit’s shaking action can be adjusted from
approximately 8 to 25 strokes per minute or the unit can
be operated with no motion as a conventional incubator.

®

or Rubbermaid® containers for hy-

PRODUCT PERFORMANCE

The Boekel Incubator Shaker II has a chamber tem­perature range of ambient plus 5°C to 70°C. The unit,
within the bounds of the shelves, has a chamber uni­formity of ± 1.3° C at 6 5° and an “in the container ” uni­formity of ± 0.7°C at 65° (temperature un iformity of the
hybridization liquid in a hea t seale d bag or Tupp erwar e®
box). The Incubator Shaker II provides three shaking
shelves which can be loaded to a maximum combined
total weight of 1 Kg (2 .2 lbs.) T he shelves are spac ed at

6.35 cm (2.5 inches) to allow the use of either heat
sealed bags or shallow Tupperware® containers.
Shelves may be removed to allo w more space for larger
containers. Shelf shaking speed is controlled by an
on/off switch and a front pane l mounted variable speed
control knob.

UNPACKING

1. Remove the Incubator Sh ak er I I and tray set f rom
the carton.

2. Inspect for damage. Repor t all shipping damage
to the carrier imm ediately. Shipping dam ages are
covered by the carr ier and repair and/or replace­ment for shipping d amages must be coordinated
through the carrier.

3. Retain all packaging m aterial i n the event t hat th e
unit must be returned.

4. Completely fill out and ret urn the self-addressed
Boekel Warranty Registration Card to Boekel
Scientific.

SET-UP

1. Place the unit in a c lear, draft fr ee environment.
Ensure that the surface on which the unit is
placed will withstan d the radiated heat produced
by typical laboratory incubators. Ensure that the
side ventilation holes are not blocked.

2. Plug the power cord into a grounded receptacle
of appropriate voltage.

3. Place the heat sealed hybridization bag directly
on the shelf or on th e supplied tray. Then place
the tray on the shelf. Note: Us e of the trays may
diminish chamber uniformity due to temperature
stratification.

®

Tupperware
be used on the lower two shelves. However, to prevent
shelf jamming, use a box no higher than 3.3 cm (1.3
inches) on the top shelf of Incubator Shak er II. Boxes
taller than 3.3 cm m ay be used on the top shelf onl y if
they can be placed to th e right hand side of the s helf so
that they do not jam agains t the chamber ceiling on the
upward stroke of the shelf.

boxes up to 6 .35 cm ( 2.5 inches) high m ay

OPERATION

1. Turn the “Power” switch ON. Ambient chamber
temperature will be displa yed on the digital read­out.

2. Turn the “Shaker” switch on to start the shelf
rocking ac tion. I ncrease t he shak ing spee d by r o­tating the “Shak er Speed” knob in the clockwise
direction.

Setting Temperature

The Temperature Contro ller has three butto ns . When the
button on the left is depressed it will display “Target
Temperature”. W hen the left button is depressed s imul­taneously with the middle button, the “Target Tem­perature” value is lowered. When the left button is de­pressed simultaneous ly with the button on the rig ht, the
“Target Temperat ure” is increas ed. When all buttons are
released, actual chamber temperature is displayed. In
the event of power loss, the Temperature Controller r e­tains last “Target Temperature” value. The flickering
green dot in the upper left cor ner of the Controller Dis­play is the heat indicator light.

N2400040 REV 1 05/11

CLEANING

1. Disengage power cord before performing any
cleaning.

2. The interior bottom, sides, shelves and trays of
the unit can be clea ned using a suitab le cleaning
and disinfecting solut ion. Allow to dry thoroughly
before restarting unit.

3. The exterior can be c leaned with a damp cloth or
sponge.

4. Be sure to prop erly dispose of all cle an ing materi­als, especially any radioactive items.

SERVICE: RETURN AUTHORIZATION I S
REQUIRED.

Should service be req uired, contac t your salesperson
or call Boekel Scientific Customer Service.

NOTE: Before shipping this unit for service, the

must

unit
taminated of any radioactivity. Shipment of
unmarked radioactive units is illegal. All in­coming units will be tested for radioactivity.
Disposal and/or decontamination costs will
be charged for any radioactive unit returned
for repair.

SPECIFICATIONS

be thoroughly cleaned and decon-

Size:

Exterior: 14.5”D (37 cm) x 16”H (41 cm) x
14W (36 cm)
Chamber: 11.5”D (29 cm) x 10.5”H (27 cm) x

11.5W (29 cm)
Tray: 8”D (20 cm) x 0.5”H (1.3 cm) x

8.5W (22 cm)
Shelf Separation: 2.5” (6.35 cm)
Shelf Area: 9”D (23 cm) x 9.5”W (24 cm)
Temperature Range: Ambient plus 5°C to 70°C Opera-

tion above 70°C not recommended.
Shaking Speed: 8 to 25 strokes per minute, typical
Weight: 24 lbs (11 kg)
Power: Model 136400, 136400S 115 VAC, 230 watts
Model 136400-2 230 VAC, 230 watts

TYPICAL CHAMBER TEMPERATURE PERFORMANCE

Condition

1

Chamber Stability

2

Chamber Uniformity

3

37°C without trays 37°C ±0.2°C ±0.5°C

37°C with trays 37°C ±0.5°C ±1.2°C

65°C without trays 65°C ±1.0°C ±1.3°C

65°C with trays 65°C ±1.0°C ±2.3°C

65°C in sealed bag or box on shelf – Uniformity of 65°C ±0.7°C

1 – With trays refers to brown plastic drip trays supplied with unit. When placed on shelves, trays can create some
temperature stratification.
2 – Chamber average of 5 test points within the shelf boundaries of the unit during operation.
3 – Greatest variation between 5 test points at any instant in time.

Boekel Scient if ic

855 Pennsylvania Blvd.

Feasterville, PA 19053

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