Biosearch Technologies KASP-TF V5.0 Master Mix User manual

Manual

KASP-TF V5.0 Master Mix

For Research Use Only. Not for use in diagnostic procedures.

Manual

KASP-TF V5.0 Master Mix

Contents

1. Productdescriptionandspecication 3

2. Kit contents and storage 3

3. Before you start 3

4. Prepare the KASP genotyping mix 4

5. Dispensing 5

6. KASP cycling conditions 5

7. Reading KASP reactions 6
Appendix A: Performing the KASP thermal cycle on the IntelliQube instrument 7

For Research Use Only. Not for use in diagnostic procedures.

Manual

KASP-TF V5.0 Master Mix

1. Productdescriptionandspecication

KASP-TF V5.0 Master Mix by LGC, Biosearch Technologies™ has been optimised for high-throughput

single nucleotide polymorphism (SNP) and insertion/deletion (indel) genotyping in Array Tape™. This 2X

mix has been designed specically for use with Array Tape on the Nexar™ in-line liquid handling and assay

processing system (Nexar system) and the IntelliQube™ instrument.

In high-throughput experiments, users typically perform thermal cycling of Array Tape using a

Hydrocycler2™ or a Soellex™. It is possible to perform thermal cycling of Array Tape on the IntelliQube

instrument; please see Appendix A for details.

KASP-TF V5.0 Master Mix is available in three pack sizes, as detailed in Table 1.

Product code Pack size Pack size

KBS-1050-201 2.5 mL KASP-TF V5.0 2X Master Mix, Standard ROX

KBS-1050-202 25 mL KASP-TF V5.0 2X Master Mix, Standard ROX

KBS-1050-204 250 mL KASP-TF V5.0 2X Master Mix, Standard ROX

KBS-1050-203

Table 1. Product codes and pack sizes for KASP-TF V5.0 Master Mix.

2. Kit contents and storage

i. KASP-TF V5.0 Master Mix – supplied at 2X concentration. Contains FAM™ and HEX™ specic

FRET cassettes, KASP Taq , dNTPs, salts, and buffer.
ii. MgCl2 – 50 mM, for optimisation of assays in particularly A/T-rich DNA regions.
iii. DMSO – for optimisation of assays in particularly G/C-rich DNA regions.

KASP-TF V5.0 Master Mix can be safely stored for 1 week at 4 °C, one year at -20 °C, or indenitely at

-80 °C. KASP-TF V5.0 Master Mix should be aliquoted for storage, and use of light-protective tubes is
recommended. Frequent freezing and thawing of KASP-TF V5.0 Master Mix is not recommended.

(250 mL)
(10 × 25 mL)

KASP-TF V5.0 2X Master Mix, Standard ROX

3. Before you start

• KASP Assay Mix should be ordered from Biosearch Technologies. Each KASP Assay Mix is specic

to the SNP or indel that is to be targeted, and consists of two competitive allele-specic primers and

one common reverse primer. Two options are available for ordering KASP Assay Mix:

– KASP-by-Design (KBD) – primers based on in silico design, no wet lab validation.
– KASP-on-Demand (KOD) – optimised and functionally validated in our genotyping laboratory.

3

For Research Use Only. Not for use in diagnostic procedures.