Biosearch Technologies KASP User Manual

User guide

KASP end-point genotyping trial kit

For Research Use Only. Not for use in diagnostic procedures.

User guide

KASP end-point genotyping trial kit

Contents

1. Introduction

2. Kit contents

3. Experimental procedure for performing KASP end point genotyping reactions

3.1 KASP genotyping reactions

3.2 Kit assembly

3.3 KASP genotyping mix assembly

3.4 Experimental procedure

3.5 Thermal cycling conditions

3.6 Additional cycling conditions

4. Ordering information

5. Technical support

User guide

KASP end-point genotyping trial kit

1. Introduction

The KASP™ genotyping trial kit, from LGC, Biosearch Technologies™, is supplied as an introduction
to the use of KASP chemistry. It enables the user to trial the chemistry in their own laboratory using
existing equipment.

In general, any qPCR instrument or plate reader that is capable of reading FRET at the wavelengths in
Table 1 should be compatible with KASP.

KASP has been validated on a wide range of qPCR instruments including:

• ABI 7500

• ABI 7300

• ABI 7900

• ABI ViiA7

• Roche LC480

• Agilent Mx3000P/3005P

• illumina EcoRT

• BIO-RAD CFX

Please visit our website to view a full list of the instruments that have been validated for KASP

chemistry to date, to access our instrument-specic manuals, and to determine the correct version of

KASP-TF Master Mix for your machine.

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