Bio-Rad ZOE Fluorescent Cell Imager User Manual

ZOE™ Fluorescent Cell Imager

Instruction Manual

Catalog #145-0031

Bio-Rad Technical Support

For help and technical advice, please contact the Bio-Rad Technical Support department. In the United
States, the Technical Support department is open Monday–Friday, 5:00 AM–5:00 PM, Pacific time.

www.bio-rad.com

Bio-Rad Laboratories
Life Science Research
2000 Alfred Nobel Drive
Hercules, CA 94547

Telephone: [phone]
Toll Free: 1-800-4-BIORAD (1-800-424-6723)

Fax: [phone]
Free Fax: [phone]

Online technical support and worldwide contact information are available at www.consult.bio-rad.com.

Legal Notices

Bio-Rad reserves the right to modify its products and services at any time. This instruction manual is subject
to change without notice.

Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors, or for any damages resulting
from the application or use of this information.

Copyright © 2014 by Bio-Rad Laboratories, Inc. All rights reserved. No part of this publication may be reproduced or transmitted in
any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage or retrieval system,
without permission in writing from Bio-Rad.

ZOE Fluorescent Cell Imager Instruction Manual | i

Bio-Rad Laboratories Resources

Bio-Rad provides many resources for scientists. Bio-Rad resources and how to locate what you need are listed below.

Bio-Rad resources.

Resource How to Contact

Local Bio-Rad Laboratories Find local information and contacts on the Bio-Rad Laboratories website by selecting your country
representatives on the homepage (www.bio-rad.com). Find the nearest international office listed on the back of

this manual

Technical support scientists Bio-Rad’s technical support scientists provide our customer with practical and expert solutions.

To find local technical support on the phone, contact your nearest Bio-Rad office. For technical
support in the United States and Canada, call 1-800-424-6723 (toll-free), and select the technical
support option

Service support engineers Maintenance and repairs should be carried out only by authorized service support engineers

For service support in the United States and Canada, call 1-800-424-6723 (toll-free), and select

the technical support option to request service support

Technical notes and literature Go to the Bio-Rad website (www.bio-rad.com). Type a term in the Search box and select

Documents tab to find links to literature

Warranty

The ZOE Fluorescent Cell Imager and associated accessories are covered by a standard Bio-Rad warranty. Contact your local Bio-Rad
Laboratories office for the details of the warranty.

Safety Use Specifications and Compliance

A Warning! label in this manual warns you about sources of injury or harm, including risk of electrical shock.

Warning! Do not attempt to repair or remove the outer case of the ZOE inverted cell imager or other accessories. If you open this

instrument, you put yourself at risk for harm to body or equipment from electrical shock.

This instrument has been tested and found to be in compliance with all applicable requirements of the following safety and
electromagnetic standards.

Environmental conditions for safe operation of the ZOE
Fluorescent Cell Imager.

Transient category Installation category II
Operating power 100–240 V AC
Frequency 50/60 Hz
Electrical input 24 V DC, 100 W
Installation site Indoor use only
Operating temperature 10–31°C
Maximum relative humidity 20–80%
Altitude <2,000 m
Pollution degree 2
Degree of protection IPX0

Note: Do not store or operate the unit near a sink as contact with water could cause electric shock.

Unit is heavy, do not store or operate it at the edge of a laboratory bench. Unit must be in upright position during operation.

Condensation will form on any object when the temperature of the object is at or below the dew point temperature of the air
surrounding the object. If the instrument has been taken from a cold environment to a warmer environment, allow the instrument to
equilibrate to above the dew point temperature before operating.

Do not tip the unit over while a sample is located on the sample stage. This could cause accidental spillage of sample into the ZOE
Fluorescent Cell Imager’s optical and electronic systems, causing electrical shock and rendering unit inoperable.

Do not operate if the glass layer on the LCD screen is broken; this could injure the operator.

To prevent injury from movement of motorized stage, do not put fingers into the stage opening while the unit is powered on.

The angle on LCD screen is adjustable, keep fingers away from the back of the screen when folding down the screen. The ZOE
Fluorescent Cell Imager is factory calibrated; no further calibration is needed.

Do not connect the ZOE Fluorescent Cell Imager to a PC, it may render the device’s operating system unusable.

ZOE Fluorescent Cell Imager Instruction Manualii |ii |

Safety Compliance

This instrument has been tested and found to be in compliance with all applicable requirements of the following safety and
electromagnetic standards:

n

EN 61010-1:2010 — Electrical Equipment for Measurement, Control, and Laboratory Use

n

UL Std No. 61010-1:2012 — Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements

n

CAN/CSA C22.2 No. 61010-1-12 — Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use,

Part 1: General Requirements

n

IEC 61010-1:2010 — Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1

General Requirements

n

IEC62471 Photobiological Safety of Lamps and Lamp Systems

n

EN62471 Photobiological Safety of Lamps and Lamp Systems

Electromagnetic Compatibility (EMC)

n

F.C.C. Title 47 Part 15B as a Class A digital device

n

IEC/EN61326-1 Class A Electrical Equipment for Measurement, Control, and Laboratory Use — General Requirements

FCC Warning and Notes

n

Warning: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the

user’s authority to operate the equipment

n

Note: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of

the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment
is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation
of this equipment in a residential area is likely to cause harmful interference, in which case the user will be required to correct the
interference at the user’s own expense

n

Note regarding FCC compliance: Although this design of instrument has been tested and found to comply with Part 15, Subpart

B, of the FCC Rules for a Class A digital device, please note that this compliance is voluntary, as the instrument qualifies as an
“exempted device” under 47 CFR 15.103(c) in regard to the cited FCC regulations in effect at the time of manufacture

n

Note regarding Canadian EMC compliance: Le present appareil numerique n’emet pas de bruits radioelectrique depassant les

limites applicables aux appareils numeriques de classe A prescrites dans le reglement sur le brouillage radioelectrique edicte par le
Ministere des Communications du Canada

Canada Warning and Notes

This ISM device complies with Canadian ICES-001.
Cet appareil ISM est conforme à la norme NMB-001 du Canada.

This Bio-Rad instrument is designed and certified to meet EN61010* and the EMC requirements of EN61326 (for Class A) safety
standards. Certified products are safe to use when operated in accordance with the instruction manual. This instrument should not
be modified or altered in any way. Alteration of this instrument will cause the following results:

n

Void the manufacturer’s warranty

n

Void the EN61010 safety certification

n

Create a potential safety hazard

Bio-Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those
for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent. We
strongly recommend that you follow the safety specifications listed in this section and throughout this manual. Use only the supplied
power cord in the instrument, making sure to choose the plug adaptor that corresponds to the electrical outlets in your region.

| iiiZOE Fluorescent Cell Imager Instruction Manual | iii

ZOE Fluorescent Cell Imager Instruction Manualiv |iv |

Table of Contents

Chapter 1: Introduction ................................. 1

Instrument Overview .................................... 2

Graphical User Interface (GUI) ............................. 2

Specifications ......................................... 3

Chapter 2: Setting Up the Instrument ..................... 5

Chapter 3: Viewing Cells; Capturing and

Processing Cell Images ................................ 7

Position Mode ......................................... 7

Live Mode ............................................ 8

Image Capture ........................................12

Gallery Mode .........................................12

Merge Mode ..........................................15

Capturing Images for Multicolor Merge ......................17

Chapter 4: Maintenance and Troubleshooting ..............19

Cleaning the ZOE Cell Imager .............................19

Battery ..............................................20

Chapter 5: Software Notices and Terms .................. 21

Ordering Information.................................. 25

| vZOE Fluorescent Cell Imager Instruction Manual | v

ZOE Fluorescent Cell Imager Instruction Manualvi |

1

Introduction

The ZOE Fluorescent Cell Imager is an inverted imaging system with brightfield and three
fluorescent channels (emitting in blue, green, and red color), suitable for routine cell culture
and imaging applications. All channels are fully integrated and optimized for most commonly
used fluorescent proteins and dyes; no calibration or installation is needed. It is a stand-alone
instrument and a PC is not needed to operate it.

The intuitive touch screen allows researchers to visualize their samples and capture cell
images with the integrated digital complementary metal-oxide semiconductor (CMOS) camera.
Images stored in the internal memory can be edited, overlaid into multicolor merges, and/or
exported to a USB key using one of the two USB ports. The integrated light shield removes the
need for a dark room and allows visualization of fluorescence in ambient light on the bench,
where researchers work with cells. The screen viewing angle can be adjusted easily and
optimized to a user’s body height.

Each LED light source provides thousands of hours of reliable illumination that are instantly
ready after power on. They provide cool, even, and continuous illumination; their brightness
can be adjusted by the user to reduce sample photobleaching.

Focusing is conducted manually using coaxial coarse/fine focus knobs located on the instrument
base in an ergonomically deduced position, thus minimizing the strain on the user’s hands.

The ZOE Cell Imager’s 20X lens is mounted in a proprietary manner that results in a large field
of view, one that is approximately equivalent to that of a 4X objective lens (0.70 mm
the pinch-to-zoom gesture, researchers can instantly change magnification while retaining
resolution of 1 µm.

Thanks to the large field of view and a motorized stage (up to 6 mm of travel), a large sample
area can be visualized rapidly. This attribute is useful when assessing transfection efficiency
or cell confluency. The direction and speed of the stage’s movement are controlled through
the touch screen.

ZOE Fluorescent Cell Imager Instruction Manual | 1

2

). Using

Instrument Overview

The front panel of the ZOE cell imager (Figure 1) includes:

n

Screen — 10.1", high-resolution, color LCD touch screen with antiglare and antifingerprint
coatings; screen angle can be adjusted to optimize viewing experience

n

Light shield — allows use of fluorescent channels in ambient light

n

Sample stage — positioned on top of the instrument base, sample stage is large enough
to fit any of the commonly used cell culture vessels

n

Objective opening — located in the center of the sample stage, objective lens is below
the opening

n

USB port — for connecting USB flash drive

n

Indicator light — glows green when instrument is powered on. If light is not on, instrument
is powered off

The right side panel (Figure 1) includes:

n

Power switch — for turning the instrument on and off

n

Focus knobs — for manually adjusting image focus; outer knob adjusts coarse focus, inner
knob adjusts fine focus

The left side panel includes:

n

F ocus knobs — manual-adjust image focus; outer knob adjusts coarse focus, inner knob

adjusts fine focus

The rear panel (Figure 1, inset) includes:

n

USB port — for connecting USB flash drive

n

Power inlet — for connecting the imager to an electrical outlet via the supplied power cord
(select the cable with appropriate plug adaptor for your region)

Note: Hold the power cord with its flat side facing down; push into the power inlet without
blocking the locking mechanism. To remove, pull the locking mechanism to unlock the cable.
Pulling the cable without releasing the locking mechanism can result in instrument damage.

Graphical User Interface (GUI)

The touch screen commands include four functions:

n

Position — select the target sample area for cell imaging; view of the entire vessel is shown
on the LCD screen with the center of the crosshair located above the objective lens (Figure 2)

n

Live — view samples using brightfield (Figure 3) or any of the three fluorescent channels; use
this mode for image acquisition

n

Gallery — for accessing captured images stored in the internal memory

n

Merge — for creating multicolor image overlays

2 | ZOE Fluorescent Cell Imager Instruction Manual2 |

Touch screen
with antiglare and
antifingerprint
treatment

Light shield

Motorized
sample stage

Power inlet USB port

USB port

Fig. 1. Elements of the ZOE Fluorescent Cell Imager. Though not shown in full instrument image, both left and
right sides have focus knobs. (They can be seen in inset image of the back of the instrument.)

Specifications

The specifications for the ZOE Fluorescent Cell Imager are shown in Table 1.

Table 1. ZOE Fluorescent Cell Imager specifications.

Color channels Brightfield*, blue, green, and red fluorescence channels

Illumination UV LED for blue channel
Blue LED for green channel
Green LED for red channel

Excitation Blue channel 355/40 nm
Green channel 480/17 nm
Red channel 556/20 nm

Emission Blue channel 433/36 nm
Green channel 517/23 nm
Red channel 615/61 nm

Numerical aperture 0.40

Working distance 7 mm

Objective 20X plan achromatic

Display magnification 175X

Digital zoom magnification 700X

Motorized stage 6 mm travel in X, Y direction, touch screen control of travel speed and direction

Power
switch

Coaxial coarse/fine
focus knob

continues

| 3ZOE Fluorescent Cell Imager Instruction Manual | 3