ZOE™ Fluorescent Cell Imager
Instruction Manual
Catalog #145-0031
Bio-Rad Technical Support
For help and technical advice, please contact the Bio-Rad Technical Support department. In the United
States, the Technical Support department is open Monday–Friday, 5:00 AM–5:00 PM, Pacific time.
www.bio-rad.com
Bio-Rad Laboratories
Life Science Research
2000 Alfred Nobel Drive
Hercules, CA 94547
Telephone: 510-741-1000
Toll Free: 1-800-4-BIORAD (1-800-424-6723)
Fax: 510-741-5800
Free Fax: 1-800-879-2289
Online technical support and worldwide contact information are available at www.consult.bio-rad.com.
Legal Notices
Bio-Rad reserves the right to modify its products and services at any time. This instruction manual is subject
to change without notice.
Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors, or for any damages resulting
from the application or use of this information.
Copyright © 2014 by Bio-Rad Laboratories, Inc. All rights reserved. No part of this publication may be reproduced or transmitted in
any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage or retrieval system,
without permission in writing from Bio-Rad.
ZOE Fluorescent Cell Imager Instruction Manual | i
Bio-Rad Laboratories Resources
Bio-Rad provides many resources for scientists. Bio-Rad resources and how to locate what you need are listed below.
Bio-Rad resources.
Resource How to Contact
Local Bio-Rad Laboratories Find local information and contacts on the Bio-Rad Laboratories website by selecting your country
representatives on the homepage (www.bio-rad.com). Find the nearest international office listed on the back of
this manual
Technical support scientists Bio-Rad’s technical support scientists provide our customer with practical and expert solutions.
To find local technical support on the phone, contact your nearest Bio-Rad office. For technical
support in the United States and Canada, call 1-800-424-6723 (toll-free), and select the technical
support option
Service support engineers Maintenance and repairs should be carried out only by authorized service support engineers
For service support in the United States and Canada, call 1-800-424-6723 (toll-free), and select
the technical support option to request service support
Technical notes and literature Go to the Bio-Rad website (www.bio-rad.com). Type a term in the Search box and select
Documents tab to find links to literature
Warranty
The ZOE Fluorescent Cell Imager and associated accessories are covered by a standard Bio-Rad warranty. Contact your local Bio-Rad
Laboratories office for the details of the warranty.
Safety Use Specifications and Compliance
A Warning! label in this manual warns you about sources of injury or harm, including risk of electrical shock.
Warning! Do not attempt to repair or remove the outer case of the ZOE inverted cell imager or other accessories. If you open this
instrument, you put yourself at risk for harm to body or equipment from electrical shock.
This instrument has been tested and found to be in compliance with all applicable requirements of the following safety and
electromagnetic standards.
Environmental conditions for safe operation of the ZOE
Fluorescent Cell Imager.
Transient category Installation category II
Operating power 100–240 V AC
Frequency 50/60 Hz
Electrical input 24 V DC, 100 W
Installation site Indoor use only
Operating temperature 10–31°C
Maximum relative humidity 20–80%
Altitude <2,000 m
Pollution degree 2
Degree of protection IPX0
Note: Do not store or operate the unit near a sink as contact with water could cause electric shock.
Unit is heavy, do not store or operate it at the edge of a laboratory bench. Unit must be in upright position during operation.
Condensation will form on any object when the temperature of the object is at or below the dew point temperature of the air
surrounding the object. If the instrument has been taken from a cold environment to a warmer environment, allow the instrument to
equilibrate to above the dew point temperature before operating.
Do not tip the unit over while a sample is located on the sample stage. This could cause accidental spillage of sample into the ZOE
Fluorescent Cell Imager’s optical and electronic systems, causing electrical shock and rendering unit inoperable.
Do not operate if the glass layer on the LCD screen is broken; this could injure the operator.
To prevent injury from movement of motorized stage, do not put fingers into the stage opening while the unit is powered on.
The angle on LCD screen is adjustable, keep fingers away from the back of the screen when folding down the screen. The ZOE
Fluorescent Cell Imager is factory calibrated; no further calibration is needed.
Do not connect the ZOE Fluorescent Cell Imager to a PC, it may render the device’s operating system unusable.
ZOE Fluorescent Cell Imager Instruction Manualii |ii |
Safety Compliance
This instrument has been tested and found to be in compliance with all applicable requirements of the following safety and
electromagnetic standards:
n
EN 61010-1:2010 — Electrical Equipment for Measurement, Control, and Laboratory Use
n
UL Std No. 61010-1:2012 — Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements
n
CAN/CSA C22.2 No. 61010-1-12 — Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use,
Part 1: General Requirements
n
IEC 61010-1:2010 — Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1
General Requirements
n
IEC62471 Photobiological Safety of Lamps and Lamp Systems
n
EN62471 Photobiological Safety of Lamps and Lamp Systems
Electromagnetic Compatibility (EMC)
n
F.C.C. Title 47 Part 15B as a Class A digital device
n
IEC/EN61326-1 Class A Electrical Equipment for Measurement, Control, and Laboratory Use — General Requirements
FCC Warning and Notes
n
Warning: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the
user’s authority to operate the equipment
n
Note: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of
the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment
is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation
of this equipment in a residential area is likely to cause harmful interference, in which case the user will be required to correct the
interference at the user’s own expense
n
Note regarding FCC compliance: Although this design of instrument has been tested and found to comply with Part 15, Subpart
B, of the FCC Rules for a Class A digital device, please note that this compliance is voluntary, as the instrument qualifies as an
“exempted device” under 47 CFR 15.103(c) in regard to the cited FCC regulations in effect at the time of manufacture
n
Note regarding Canadian EMC compliance: Le present appareil numerique n’emet pas de bruits radioelectrique depassant les
limites applicables aux appareils numeriques de classe A prescrites dans le reglement sur le brouillage radioelectrique edicte par le
Ministere des Communications du Canada
Canada Warning and Notes
This ISM device complies with Canadian ICES-001.
Cet appareil ISM est conforme à la norme NMB-001 du Canada.
This Bio-Rad instrument is designed and certified to meet EN61010* and the EMC requirements of EN61326 (for Class A) safety
standards. Certified products are safe to use when operated in accordance with the instruction manual. This instrument should not
be modified or altered in any way. Alteration of this instrument will cause the following results:
n
Void the manufacturer’s warranty
n
Void the EN61010 safety certification
n
Create a potential safety hazard
Bio-Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those
for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent. We
strongly recommend that you follow the safety specifications listed in this section and throughout this manual. Use only the supplied
power cord in the instrument, making sure to choose the plug adaptor that corresponds to the electrical outlets in your region.
| iiiZOE Fluorescent Cell Imager Instruction Manual | iii
ZOE Fluorescent Cell Imager Instruction Manualiv |iv |
Table of Contents
Chapter 1: Introduction ................................. 1
Instrument Overview .................................... 2
Graphical User Interface (GUI) ............................. 2
Specifications ......................................... 3
Chapter 2: Setting Up the Instrument ..................... 5
Chapter 3: Viewing Cells; Capturing and
Processing Cell Images ................................ 7
Position Mode ......................................... 7
Live Mode ............................................ 8
Image Capture ........................................12
Gallery Mode .........................................12
Merge Mode ..........................................15
Capturing Images for Multicolor Merge ......................17
Chapter 4: Maintenance and Troubleshooting ..............19
Cleaning the ZOE Cell Imager .............................19
Battery ..............................................20
Chapter 5: Software Notices and Terms .................. 21
Ordering Information.................................. 25
| vZOE Fluorescent Cell Imager Instruction Manual | v
ZOE Fluorescent Cell Imager Instruction Manualvi |
1
Introduction
The ZOE Fluorescent Cell Imager is an inverted imaging system with brightfield and three
fluorescent channels (emitting in blue, green, and red color), suitable for routine cell culture
and imaging applications. All channels are fully integrated and optimized for most commonly
used fluorescent proteins and dyes; no calibration or installation is needed. It is a stand-alone
instrument and a PC is not needed to operate it.
The intuitive touch screen allows researchers to visualize their samples and capture cell
images with the integrated digital complementary metal-oxide semiconductor (CMOS) camera.
Images stored in the internal memory can be edited, overlaid into multicolor merges, and/or
exported to a USB key using one of the two USB ports. The integrated light shield removes the
need for a dark room and allows visualization of fluorescence in ambient light on the bench,
where researchers work with cells. The screen viewing angle can be adjusted easily and
optimized to a user’s body height.
Each LED light source provides thousands of hours of reliable illumination that are instantly
ready after power on. They provide cool, even, and continuous illumination; their brightness
can be adjusted by the user to reduce sample photobleaching.
Focusing is conducted manually using coaxial coarse/fine focus knobs located on the instrument
base in an ergonomically deduced position, thus minimizing the strain on the user’s hands.
The ZOE Cell Imager’s 20X lens is mounted in a proprietary manner that results in a large field
of view, one that is approximately equivalent to that of a 4X objective lens (0.70 mm
the pinch-to-zoom gesture, researchers can instantly change magnification while retaining
resolution of 1 µm.
Thanks to the large field of view and a motorized stage (up to 6 mm of travel), a large sample
area can be visualized rapidly. This attribute is useful when assessing transfection efficiency
or cell confluency. The direction and speed of the stage’s movement are controlled through
the touch screen.
ZOE Fluorescent Cell Imager Instruction Manual | 1
2
). Using
Instrument Overview
The front panel of the ZOE cell imager (Figure 1) includes:
n
Screen — 10.1", high-resolution, color LCD touch screen with antiglare and antifingerprint
coatings; screen angle can be adjusted to optimize viewing experience
n
Light shield — allows use of fluorescent channels in ambient light
n
Sample stage — positioned on top of the instrument base, sample stage is large enough
to fit any of the commonly used cell culture vessels
n
Objective opening — located in the center of the sample stage, objective lens is below
the opening
n
USB port — for connecting USB flash drive
n
Indicator light — glows green when instrument is powered on. If light is not on, instrument
is powered off
The right side panel (Figure 1) includes:
n
Power switch — for turning the instrument on and off
n
Focus knobs — for manually adjusting image focus; outer knob adjusts coarse focus, inner
knob adjusts fine focus
The left side panel includes:
n
F ocus knobs — manual-adjust image focus; outer knob adjusts coarse focus, inner knob
adjusts fine focus
The rear panel (Figure 1, inset) includes:
n
USB port — for connecting USB flash drive
n
Power inlet — for connecting the imager to an electrical outlet via the supplied power cord
(select the cable with appropriate plug adaptor for your region)
Note: Hold the power cord with its flat side facing down; push into the power inlet without
blocking the locking mechanism. To remove, pull the locking mechanism to unlock the cable.
Pulling the cable without releasing the locking mechanism can result in instrument damage.
Graphical User Interface (GUI)
The touch screen commands include four functions:
n
Position — select the target sample area for cell imaging; view of the entire vessel is shown
on the LCD screen with the center of the crosshair located above the objective lens (Figure 2)
n
Live — view samples using brightfield (Figure 3) or any of the three fluorescent channels; use
this mode for image acquisition
n
Gallery — for accessing captured images stored in the internal memory
n
Merge — for creating multicolor image overlays
2 | ZOE Fluorescent Cell Imager Instruction Manual2 |
Touch screen
with antiglare and
antifingerprint
treatment
Light shield
Motorized
sample stage
Power inlet USB port
USB port
Fig. 1. Elements of the ZOE Fluorescent Cell Imager. Though not shown in full instrument image, both left and
right sides have focus knobs. (They can be seen in inset image of the back of the instrument.)
Specifications
The specifications for the ZOE Fluorescent Cell Imager are shown in Table 1.
Table 1. ZOE Fluorescent Cell Imager specifications.
Color channels Brightfield*, blue, green, and red fluorescence channels
Illumination UV LED for blue channel
Blue LED for green channel
Green LED for red channel
Excitation Blue channel 355/40 nm
Green channel 480/17 nm
Red channel 556/20 nm
Emission Blue channel 433/36 nm
Green channel 517/23 nm
Red channel 615/61 nm
Numerical aperture 0.40
Working distance 7 mm
Objective 20X plan achromatic
Display magnification 175X
Digital zoom magnification 700X
Motorized stage 6 mm travel in X, Y direction, touch screen control of travel speed and direction
Power
switch
Coaxial coarse/fine
focus knob
continues
| 3ZOE Fluorescent Cell Imager Instruction Manual | 3
Table 1. ZOE Fluorescent Cell Imager specifications. (cont.)
FOV 0.70 mm
Camera Monochrome, 12 bit CMOS, 5 megapixels
Image format JPEG, TIFF, RAW (8 and 12-bit)
Internal memory 16 GB
Focus Coarse and fine, manual adjustment
Stand-alone instrument Yes
LCD monitor 10.1", color, LCD, touch screen with antiglare and antifingerprint treatment,
1,280 x 800 pixels image resolution, 80–180° angle tilt range
Ports 2 USB ports
Dimensions 33 x 32 x 30 cm (13 x 12.6 x 11.6")
Weight 9 kg (19.7 lb)
* Brightfield channel is illuminated with green LEDs that improve image contrast by reducing chromatic aberration.
Photobleaching due to this green light source is comparable to that encountered with a regular white light.
Note: User interface controls for individual channels are labeled according to the emission colors (blue, green, and red).
2
System Components
Catalog #145-0031 includes:
n
ZOE Fluorescent Cell Imager
n
Power cord
n
USB key
n
Instruction manual
n
Quick guide
Unpacking the System Components
1. Unpack the ZOE Cell Imager carefully. Remove all packaging materials and store them for
future use. Examine the instrument carefully for any damage incurred during transit. Ensure
that all parts of the instrument listed above are included with the product. If any item is
missing or damaged, contact your local Bio-Rad office.
2. Place the imager in an upright position on a dry, level surface.
3. Thanks to its light shield, the ZOE Cell Imager can be used in ambient light; dark room
operation is not required. However, it is not designed to be operated in direct sunlight.
4. Insert the supplied power cord into the instrument with its flat side facing down and without
blocking the locking jacket.
Note: To remove, pull the locking jacket to unlock the cable. Pulling the cable without unlocking
this mechanism can result in instrument breakage.
5. Plug the power cord into the appropriate electrical outlet and the instrument will power on.
The loading screen displays. After the loading sequence is completed the Position mode will
appear onscreen.
Note: Turning the unit off by pressing the Power off button results in only partial shutoff. For
complete power off remove power cord from electrical outlet.
4 | ZOE Fluorescent Cell Imager Instruction Manual4 |
2
Setting Up the Instrument
Menus on the touch screen can be customized. To change the default instrument settings, tap
the icon located in the upper left corner of the screen (Figure 2) and select Settings.
1. In the Settings window that opens, tap the option you want to modify.
2. Make the desired modifications and tap
Options on the following menus can be modified.
Camera
The default values for Gain, Exposure time, LED Intensity, and Contrast can be changed. The
instrument must be powered off and turned back on for new values to become active.
Preferences
The following options can be modified:
Image File Format — captured images are stored as JPEGs. Alternatives include TIFF and
RAW, but the large files of these formats slow down the operating system.
Image File Name Format — default file name format is Color_Serial number_Time stamp.
Alternatively, Color_Prefix_Time stamp allows you to use a custom prefix (for example, Hela).
Add scale bar to captured image — tap Ye s to add a scale bar to captured images; tap No
to disable the function.
Attach underlying single color images to merge — tap Ye s to export underlying single-color
images along with the merge file; tap No to disable the attach function during export to a
US B key.
Automatic export of image files to USB drive — enable automated image export to inserted
USB flash drive. Images will be saved to both the USB drive and the internal memory.
Sleep Mode — determine how long the unit should stay fully on after a user interaction. In
sleep mode certain functionalities are turned off to preserve power. The ZOE Cell Imager comes
fully back on within 20 seconds when the screen is touched.
to exit.
Date and Time
The date/time stamp is used to track images stored in the internal memory. The date and time
should be set before using the imager. Resetting the date/time after the cell imager has been
used will not affect already stored images.
ZOE Fluorescent Cell Imager Instruction Manual | 5
System
The following information is available in this menu option:
n
Legal information
n
Android version
n
Kernel version
n
Vision board drive version
n
Driver board firmware version
n
Galileo software version
n
Firmware update
n
System test posting
Firmware update
To update firmware on the instrument:
1. Insert a USB flash drive with the firmware update file into USB port. The firmware file must
be saved in the root directory of the USB flash drive
2. Tap Firmware update in the System option of the Settings menu.
3. When the instrument recognizes and validates the firmware file, it proceeds with the update.
Note: If the USB key with firmware file is not inserted, a message prompting you to do so
will be displayed. If the update file is not saved in the root directory, a “No update file present.”
message displays.
4. Tap Enter to continue updating.
5. When the update is successfully completed, the ZOE Cell Imager automatically restarts.
Note: Three types of update files may be available: a Vision board driver update, Driver board
firmware, and/or ZOE software.
If both the Vision board driver and ZOE software are updated at the same time, the Vision board
driver will be updated first. When that is completed the instrument automatically restarts itself
and repeats steps 2–5 to update the ZOE software.
If the Driver board firmware is also present, it and any other update files are automatically
updated without repeating steps 2–5.
ZOE Fluorescent Cell Imager Instruction Manual6 |
3
Viewing Cells; Capturing and Processing Cell Images
Tap the appropriate icon, located on the right side of the touch screen (Figure 2), to access the
Position, Live, Gallery, or Merge functions.
Position Mode
The unit automatically goes into Position mode when it is powered on. The display area of the
touch screen shows the top view of the sample stage. The red crosshair is always over the
objective lens (Figure 2) and represents the actual imaging spot. Tap the Center icon to return
stage to the home position (X, Y; 0, 0) at any time.
Note: If the stage returns to center while you are viewing a sample, the sample viewing area
will change. To prevent accidental overlaying of images captured from different locations of the
sample, all images previously selected for merge (multicolor images) are cleared whenever the
stage is centered.
Settings
Access to
functional
modes
Crosshair
aligned with
objective lens
Center stage
Fig. 2. Elements of the touch screen when the ZOE Fluorescent Cell Imager is powered on. White icon shows
imager is in Position mode; other modes are grayed out.
ZOE Fluorescent Cell Imager Instruction Manual | 7
Live Mode
The Live function is used for viewing samples illuminated in one of the four channels (brightfield
or one of the three fluorescent channels) and for acquiring cell images.
Motorized Stage and Field of View
The ZOE Cell Imager’s motorized stage, with up to 6 mm of travel, enables rapid visualization
of a large sample area. Direction and speed of the stage movement are controlled through the
touch screen, and display magnification can be instantly increased using the pinch-to-zoom
gesture. X and Y coordinates (Figure 4) are updated as the stage moves. Use the Center icon
(Figure 4) to return the stage to the Home position (X,Y: 0,0).
Using the Brightfield Channel
A control panel located on the left side of the screen contains controls for switching on and off
light sources for the individual imaging channels (Figure 4). For improved image contrast, a ring
of green LEDs is the light source in the brightfield channel. ZOE uses a monochrome camera
and the resulting brightfield images are black and white.
Tap t he illumination control icon (Figure 3) to turn the LED light source on, tap again to
turn it off.
Note: Photobleaching due to this green light source is comparable to that of a regular white
light. The wavelength used does not cause the red fluorophore to fluoresce or photobleach.
Fig. 3. Brightfield channel light source on/off.
Swipe the screen to move
stage in desired direction
Settings
X,Y a xes:
2-D vertical,
horizontal
stage
positions
Z axis: Focus
position
Light
sources
Precapture
adjustments
Fig. 4. User controls available in Live mode.
Pinch-to-zoom and change
magnification
Tap to
change
functional
mode
Tap to center
motorized
stage
Tap to
capture an
image
ZOE Fluorescent Cell Imager Instruction Manual8 |
Focus position indicator on the left side of the touch screen shows the Z axis position number.
The number is updated during focusing (Figure 4). Knowing the approximate Z number for the
tissue culture vessels (Table 2) you use can speed up focusing.
Table 2. Z axis position range of tissue culture vessels.
Vessel Type Z Axis Position
Microscope slide 720 (± 100)
6-well plate 2,000 (± 100)
12-well plate 2,100 (± 100)
24-well plate 1,900 (± 100)
48-well plate 2,000 (± 100)
96-well plate 3,500 (± 200)
T25 flask 1,800 (± 200)
T75 flask 2,500 (± 200)
T225 tissue culture flask 3,200 (± 200)
Small petri dish 1,200 (± 200)
Large petri dish 1,100 (± 200)
Optimizing Imaging Parameters in Brightfield Channel
Use the Edit menu to adjust these precapture parameters (Figure 5): Gain, LED Intensity, and
Contrast values; Exposure time (msec); and quadrant illumination. Drag the slider bar to adjust a
parameter. Tap the – and + controls at either end of each slider bar for fine adjustments.
n
When searching for sample — increase Gain value for brighter signal; reduce Exposure
time for faster frame rate
n
Once the sample is identified and ready to be captured — lower Gain value to reduce
background noise; increase Exposure time to regain signal intensity
n
To further increase brightness — start by increasing LED Intensity value; follow by
increasing Exposure time if needed
n
To reduce nonspecific background signal — increase Contrast value
Fig. 5. Edit controls in the brightfield channel.
ZOE Fluorescent Cell Imager Instruction Manual | 9
Improving Image Contrast of Brightfield Channel
A ring of green LEDs is used as a light source in the brightfield channel. Using a green light
source reduces chromatic aberration and results in improved image contrast compared to
white light.
The Q1, Q2, Q3, and Q4 checkboxes at the top of the brightfield channel edit controls panel
(Figure 5) toggle quadrants of the ring of LEDs on and off. This control can be used to achieve
phase-like image contrast. Lighting only one quadrant shifts the direction of the light, resulting
in oblique illumination. The combination of on and off quadrants that provide the best imaging
performance depends on the sample.
1. Turn off three quadrants, leaving only one quadrant lit, by tapping and removing the check
mark (for ex. Q1, Q2, and Q4 are off, Q3 is on).
Note: If you leave two quadrants on, they should be adjacent quadrants, for ex. Q1 and Q2,
or Q2 and Q3, etc.
2. The imaging area will become significantly darker. Increase the Gain to compensate for loss
of illumination from the unlit quadrants.
3. If needed, increase the Contrast level.
4. Try whether using other quadrants results in better image quality for a specific sample.
5. To accept the changes, tap Apply; select Cancel to exit this dialogue window without
saving the changes.
Using the Fluorescent Channels
The ZOE Cell Imager has three fluorescent channels: blue, green, and red. Channels are named
according to the color they emit.
A control panel located on the left side of the screen (Figure 4) contains controls for switching
on and off light sources for the individual imaging channels. The viewing area shows the sample
illuminated with the selected light source; the displayed sample is pseudo-colored in the
appropriate emission color (Figure 8). Tap the illumination control (Figure 6) to turn the LED light
source on, tap it again to turn it off.
A B C
Fig. 6. Light source on/off controls. A, Blue channel light source on/off; B, green channel light
source on/off; C, red channel light source on/off.
Focus — position indicator on the left side of the display area shows the Z axis position
number and is updated during focusing (Figure 4). Noting the approximate Z number for the
tissue culture vessels you use can speed up focusing (Table 2).
10 | ZOE Fluorescent Cell Imager Instruction Manual10 |
Optimizing Imaging Parameters for Fluorescence Imaging
Use the Edit menu to adjust these precapture parameters (Figure 7): Gain, LED Intensity, and
Contrast values; Exposure time (msec); and quadrant illumination. Drag the slider bar to adjust a
parameter. Tap the – and + controls at either end of each slider bar for fine adjustments.
n
When searching for sample — increase the Gain value for brighter signal; reduce the
Exposure time to obtain a faster frame rate
n
Once the sample is identified and ready to be captured — lower the Gain value
to reduce the background noise; increase the Exposure time to regain signal intensity
as needed
n
To further increase brightness — start by increasing LED intensity value; follow by
increasing Exposure time if needed
Gain: 1
Exposure: 50
LED Intensity: 50
Contrast: 50
Apply
Fig. 7. Edit controls in fluorescent channels.
A B
Fig. 8. Samples pseudo-colored in the appropriate emission color in Live mode. A, BPAE cells, mitochondria stained with
MitoTracker red CMXRos; B, BPAE cells, F-actin stained with Alexa Fluor 488 phalloidin.
Cancel
| 11ZOE Fluorescent Cell Imager Instruction Manual | 11
Image Capture
MERGE_18_12312013_0447
To capture the image of the sample displayed in the viewing area, tap the Capture (Figure 4)
icon. The captured image will remain displayed on the screen (action mode will change from
Live to Gallery) and the image file will automatically be saved to the internal memory. Captured
images can be edited or used in multicolor image merges. For more details go to Capturing
Images for Multicolor Merge.
Gallery Mode
All captured images can be accessed in the Gallery mode. They are stored in image folders by
color: Brightfield, Blue, Green, or Red. Multicolor merges, created using the captured images,
are stored in the Merge folder.
The 16 GB internal memory can store approximately:
n
JPEG ~2,500 files
n
TIFF ~1,500 files
n
8-bit RAW ~400 files
n
12-bit RAW ~800 files
Note: Storing smaller numbers of images files (fewer than 1,000 JPEG files) will ensure rapid
functioning of the cell imager and will also lengthen the life span of the internal memory.
Viewing Captured Images
Controls for opening the individual folders are located in the control panel at the left side of the
screen (Figure 10). To open, tap a folder (Figure 9) and the most recently viewed image from
that folder will open. The viewing area will be black if a gallery folder is empty.
A B C D E
Fig. 9. Five image folders are accessible in Gallery mode. A, folder with brightfield images; B, folder with images
from the blue channel; C, folder with images from the green channel; D, folder with images from red channel; E, folder
with multicolor merged images.
Images in every folder are organized according to the time of capture. To move to the next
image, swipe the viewing area to the right. Swiping the viewing area to the left opens the
previously captured image.
12 | ZOE Fluorescent Cell Imager Instruction Manual12 |
Settings
Image
folders
Delete file
File
information
File name
Select for
merge
Merge selection
indicator
Clear all image selections
Access to
functional
modes
Edit
Pinch-to-zoom and change magnification
Fig. 10. Gallery mode. Selected folder, in this case the folder with images captured using the brightfield channel,
is illuminated.
Film strip
Export to
USB key
The film strip (Figure 10) of all images stored in a particular folder is located at the bottom part
of the screen, tap anywhere in the viewing area to hide it; tap again to bring it back. A thick
border surrounds the thumbnail corresponding to the currently opened image. Tapping another
window on the film strip will open that image.
Use the pinch-to-zoom gesture to magnify the image to view more details.
Edit — brightness and contrast can be adjusted by dragging the slider bar. For fine adjustments
tap the + and – controls.
Image file Information — tapping the
icon opens a menu with information such as file
type and time stamp.
Delete — tapping the
icon brings up a “Are you sure you want to permanently delete
<file name>?” message. Select OK to delete or Cancel to cancel deletion.
Note: If you delete a merged image, the single-color image files used to create the merge will
not be deleted.
File name — the file is automatically named at the time of capture based on the selected
naming convention. To change a file name, tap the file name twice. A virtual keyboard is
displayed. When new name is typed, tap Enter on the virtual keyboard; this hides the keyboard
and records the new file name.
Export — tap to export the currently opened image onto the inserted USB flash drive.
| 13ZOE Fluorescent Cell Imager Instruction Manual | 13
Image Merge Controls
For more details on creating merged images go to Capturing Images for Multicolor Merge.
Select for merge — tap the checkbox icon (Figure 11) to select an image for use in creating a
multicolor merge, selected images are automatically uploaded into the Merge mode.
Note: Only one image per color channel can be used. To replace a selected image with a new
one, tap the Select for merge control in the new image. This automatically overwrites the
previous selection in the particular color.
Merge selection indicator — this icon (Figure 12) shows the color of images selected to be
used in a merge. Tap the icon to move to the selected image in that particular captured
image folder.
Clear – tap to clear all existing merge selections.
A B
Select
Fig. 11. Select for merge control checkbox. A, in an unselected image in
the gallery, checkbox is dark (off); B, when the image is selected, checkbox
turns green, white checkmark appears (on).
Fig. 12. Merge selection indicator showing merged
colors. Images in the blue and green galleries are selected.
Select
Delete All Images
To access Delete all images control, tap the Settings icon in the upper left corner of the user
interface. To delete all images stored in the internal memory, select Delete all images.
14 | ZOE Fluorescent Cell Imager Instruction Manual14 |
Merge Mode
In this mode previously selected single-color images are overlaid into a multicolor image
(Figure 13). For more details on capturing and selecting images for merge, go to Capturing
Images for Multicolor Merge.
Individual
color
channels
can be
Active,
Inactive, or
Empty
Edit
Clear all merge selections
To save
a merge
into the
Gallery
Fig. 13. Merge mode.
Image controls located on the left side of the screen change their appearance depending on
whether an image from that color was selected (Figure 14).
A B C
Fig. 14. Color channel icons in Merge mode. A, active: color channel with selected image, the image is displayed;
B, inactive: color channel with selected image, the image is hidden; C, empty: color channel for which no image
was selected.
| 15ZOE Fluorescent Cell Imager Instruction Manual | 15
Tapping an active channel (image is displayed) hides the particular color from display, tapping it
again makes it visible again.
Edit — tap the color for which you wish to adjust brightness and contrast. Drag the slider bar or
tap the + and – controls for fine adjustments (Figure 15).
Note: Brightfield images cannot be edited in Merge mode. Any edits to a brightfield image
must be made in Gallery mode and saved. Edits made and saved in Gallery mode to an image
selected in Merge mode will also display in Merge.
Brightness: 0
Contrast: 0
Apply
Fig. 15. Edit dialog window in Merge mode.
Cancel
Save — when satisfied with the multicolor image tap the Save icon (Figure 13). The merged
image, along with links to the underlying single-color images, is saved to the Merge folder in the
Gallery (Figure 10).
Note: When creating a merge, only color channels that are active at the time of saving
(Figure 14A) are included. If a color is inactive and thus not displayed (Figure 14B), it will not
be included.
Clear — use to clear all selections (Figure 13). This control is also available in Gallery mode.
Note: To empty a single channel, go to the individual image folder in the Gallery and tap the
active Select icon (Figure 11). The icon will go to its off state and the particular color channel in
Merge mode will be empty (Figure 14C). To replace an image, return to Gallery mode and tap
the Select icon in the image you wish to use instead.
Note: When the stage is centered, a different field of view is displayed. To prevent users from
accidentally merging images with different fields of view, all image selections for merge are
cleared when the stage is centered.
16 | ZOE Fluorescent Cell Imager Instruction Manual16 |
Capturing Images for Multicolor Merge
Using the ZOE Fluorescent Cell Imager’s onboard software, images from up to four channels
can be overlaid into a multicolor image merge. When capturing images that will be overlaid into
a merge, all images should come from the same area of the sample and care should be taken
not to inadvertently swipe the screen and move the stage. All images used in a merge must be
of the same magnification. Use the scale bar displayed in the lower right corner of the screen
during image capture (Figure 8) as a guide.
Note: Images of different magnification can not be overlaid; an error message will be displayed.
To capture images for a multicolor merge:
1. Go to Live mode.
2. Turn on the light source you wish to use.
3. Adjust settings in Edit if desired and tap Capture.
4. The active mode switches to the Gallery mode and the just-captured image is displayed.
If satisfied with the image, tap Select for merge (Figure 16A) in the upper right corner
of the screen. The selected image is uploaded into the Merge mode. The merge selection
indicator is automatically updated to show all colors that are currently selected
(Figure 16B).
5. Return to the Live mode and repeat steps 3 and 4 for the remaining channels that you wish
to use in the merge.
6. Go to the Merge mode where all selected images are automatically overlaid (see section
Merge mode for more details).
7. Optimize brightness and contrast in Edit if desired. Tap Save to save the merge as a singleimage file that can be accessed in the Merge folder in Gallery mode. From there it can be
exported to a USB key along with the underlying single-color image files.
Note: Only one image per color channel can be used. To replace a selected image with a
new one, tap the Select for merge control in the new image. This automatically overwrites the
previous selection in that color. To clear all previous merge selections tap the Clear control.
| 17ZOE Fluorescent Cell Imager Instruction Manual | 17
A
B
18 | ZOE Fluorescent Cell Imager Instruction Manual18 |
Fig. 16. Selecting an image for merge. A, the displayed file, BPAE tubulin.jpg, file has not been
selected for merge. The merge selection indicator (upper right side of screen) shows that an image
from the blue folder has been selected for a merge. B, the displayed image (BPAE tubulin.jpg) file has
now been selected for merge. The merge selection indicator shows that images from both the blue
and the green folders have been selected and are uploaded into the merge mode.
4
Maintenance and Troubleshooting
The ZOE™ Fluorescent Cell Imager requires little maintenance for proper operation. However,
with long and constant use, the cell imager will need to be cleaned and some other
maintenance performed.
Cleaning the ZOE Cell Imager
The instrument should be cleaned on a regular schedule to remove any debris or dirt that might
interfere with proper function.
Cleaning the Instrument Body
Always turn off the instrument and disconnect the power cable before cleaning the case. Use a
soft, lint-free cloth and deionized water to wipe down the outer case. Avoid wetting the power
switch or the power jack while cleaning the case. To decontaminate the instrument body,
wipe it with a soft, lint-free cloth and 70% alcohol to clean the outer case. These cleaning
instructions apply only to the outer case and not to the LCD screen.
Warning! Never pour or spray water or other solutions directly on the instrument. Wet
components can cause electrical shock when the instrument is plugged in.
If you use a 10% bleach solution to clean or decontaminate the instrument, it may leave a residue
of bleach crystals that over time could scratch the surface. If bleach comes in contact with the
LCD screen, wipe down the screen with a damp cloth to remove any traces of bleach.
Do not spill liquids inside the cell imager. Do not overfill cell culture vessels or tip such a vessel
when placing it on the sample stage. This could render the cell imager not functional.
Cleaning the LCD Screen
Always turn off the instrument and disconnect the power cable before cleaning the LCD
screen. Use a soft, lint-free cloth lightly moistened with 70% isopropyl alcohol. Cleaning the
screen with excessive force can damage it. Wipe the screen dry immediately.
Warning! Do not use abrasive detergents or rough material because they may scratch the
control panel and display. Do not use bleach or water for cleaning the LCD screen as both leave
residues that make the screen appear hazy. If water must be used, it should be distilled water.
ZOE Fluorescent Cell Imager Instruction Manual | 19
Battery
Cleaning the Objective Lens
1. Raise the objective lens to its highest focus position.
2. Unplug the instrument.
3. Put a drop of recommended lens cleaner on lens tissues or cotton swab and gently wipe
the objective lens clean and dry.
Recommended Lens Cleaning Fluid
n
Lens Cleaner, Zeiss, #490133
n
Sparkle Optical Lens Cleaner, AmScope, #50104
Recommended Lens tissue
n
Commercial Grade Lens Tissue, Edmund Scientific Co., #52105
n
Cotton-Tipped Swab Applicators, Edmund Scientific Co., #56926
Warning! Do not put fingers into the stage hole or attempt to clean the objective lens while
moving the motorized stage.
The ZOE Fluorescent Cell Imager uses a 3 V lithium coin cell battery with a 10-year expected
lifetime to maintain the clock setting. If the date on the instrument changes without user input,
it may be an indication that the battery is getting weak. Should this occur, contact Bio-Rad
technical support for assistance with battery replacement.
Warning! Do not attempt to change the battery. Contact the Bio-Rad technical support team.
For the State of California (U.S.) only.
Perchlorate material — special handling may apply. See
www.dtsc.ca.gov/hazardouswaste/perchlorate for more information.
Perchlorate material: lithium battery contains perchlorate.
20 | ZOE Fluorescent Cell Imager Instruction Manual20 |
5
Software Notices and Terms
Open Source Licensing
Apache License, Version 2.0, January 2004
www.apache.org/licenses/
TERMS AND CONDITIONS FOR USE, REPRODUCTION, AND DISTRIBUTION
1. Definitions.
“License” shall mean the terms and conditions for use, reproduction, and distribution as defined by Sections 1
through 9 of this document.
“Licensor” shall mean the copyright owner or entity authorized by the copyright owner that is granting the License.
“Legal Entity” shall mean the union of the acting entity and all other entities that control, are controlled by, or are under
common control with that entity. For the purposes of this definition, “control” means (i) the power, direct or indirect, to
cause the direction or management of such entity, whether by contract or otherwise, or (ii) ownership of fifty percent
(50%) or more of the outstanding shares, or (iii) beneficial ownership of such entity.
“You” (or “Your”) shall mean an individual or Legal Entity exercising permissions granted by this License.
“Source” form shall mean the preferred form for making modifications, including but not limited to software source
code, documentation source, and configuration files.
“Object” form shall mean any form resulting from mechanical transformation or translation of a Source form, including
but not limited to compiled object code, generated documentation, and conversions to other media types.
“Work” shall mean the work of authorship, whether in Source or Object form, made available under the License, as
indicated by a copyright notice that is included in or attached to the work (an example is provided in the Appendix
below).
“Derivative Works” shall mean any work, whether in Source or Object form, that is based on (or derived from) the
Work and for which the editorial revisions, annotations, elaborations, or other modifications represent, as a whole,
an original work of authorship. For the purposes of this License, Derivative Works shall not include works that remain
separable from, or merely link (or bind by name) to the interfaces of, the Work and Derivative Works thereof.
“Contribution” shall mean any work of authorship, including the original version of the Work and any modifications
or additions to that Work or Derivative Works thereof, that is intentionally submitted to Licensor for inclusion in the
Work by the copyright owner or by an individual or Legal Entity authorized to submit on behalf of the copyright owner.
For the purposes of this definition, “submitted” means any form of electronic, verbal, or written communication sent
to the Licensor or its representatives, including but not limited to communication on electronic mailing lists, source
code control systems, and issue tracking systems that are managed by, or on behalf of, the Licensor for the purpose
of discussing and improving the Work, but excluding communication that is conspicuously marked or otherwise
designated in writing by the copyright owner as “Not a Contribution.”
“Contributor” shall mean Licensor and any individual or Legal Entity on behalf of whom a Contribution has been
received by Licensor and subsequently incorporated within the Work.
ZOE Fluorescent Cell Imager Instruction Manual | 21
2. Grant of Copyright License. Subject to the terms and conditions of this License, each Contributor hereby grants
to You a perpetual, worldwide, non-exclusive, no-charge, royalty-free, irrevocable copyright license to reproduce,
prepare Derivative Works of, publicly display, publicly perform, sublicense, and distribute the Work and such Derivative
Works in Source or Object form.
3. Grant of Patent License. Subject to the terms and conditions of this License, each Contributor hereby grants
to You a perpetual, worldwide, non-exclusive, no-charge, royalty-free, irrevocable (except as stated in this section)
patent license to make, have made, use, offer to sell, sell, import, and otherwise transfer the Work, where such license
applies only to those patent claims licensable by such Contributor that are necessarily infringed by their Contribution(s)
alone or by combination of their Contribution(s) with the Work to which such Contribution(s) was submitted. If You
institute patent litigation against any entity (including a cross-claim or counterclaim in a lawsuit) alleging that the Work
or a Contribution incorporated within the Work constitutes direct or contributory patent infringement, then any patent
licenses granted to You under this License for that Work shall terminate as of the date such litigation is filed.
4. Redistribution. You may reproduce and distribute copies of the Work or Derivative Works thereof in any medium,
with or without modifications, and in Source or Object form, provided that You meet the following conditions:
1. You must give any other recipients of the Work or Derivative Works a copy of this License; and
2. You must cause any modified files to carry prominent notices stating that You changed the files; and
3. You must retain, in the Source form of any Derivative Works that You distribute, all copyright, patent, trademark,
and attribution notices from the Source form of the Work, excluding those notices that do not pertain to any part
of the Derivative Works; and
4. If the Work includes a “NOTICE” text file as part of its distribution, then any Derivative Works that You distribute
must include a readable copy of the attribution notices contained within such NOTICE file, excluding those
notices that do not pertain to any part of the Derivative Works, in at least one of the following places: within a
NOTICE text file distributed as part of the Derivative Works; within the Source form or documentation, if provided
along with the Derivative Works; or, within a display generated by the Derivative Works, if and wherever such
third-par ty notices normally appear. The contents of the NOTICE file are for informational purposes only and do
not modify the License. You may add Your own attribution notices within Derivative Works that You distribute,
alongside or as an addendum to the NOTICE text from the Work, provided that such additional attribution notices
cannot be construed as modifying the License.
You may add Your own copyright statement to Your modifications and may provide additional or different license
terms and conditions for use, reproduction, or distribution of Your modifications, or for any such Derivative Works
as a whole, provided Your use, reproduction, and distribution of the Work otherwise complies with the conditions
stated in this License.
5. Submission of Contributions. Unless You explicitly state otherwise, any Contribution intentionally submitted for
inclusion in the Work by You to the Licensor shall be under the terms and conditions of this License, without any
additional terms or conditions. Notwithstanding the above, nothing herein shall supersede or modify the terms of any
separate license agreement you may have executed with Licensor regarding such Contributions.
6. Trademarks. This License does not grant permission to use the trade names, trademarks, service marks, or
product names of the Licensor, except as required for reasonable and customar y use in describing the origin of the
Work and reproducing the content of the NOTICE file.
7. Disclaimer of Warranty. Unless required by applicable law or agreed to in writing, Licensor provides the Work
(and each Contributor provides its Contributions) on an “AS IS” BASIS, WITHOUT WARRANTIES OR CONDITIONS
OF ANY KIND, either express or implied, including, without limitation, any warranties or conditions of TITLE, NONINFRINGEMENT, MERCHANTABILIT Y, or FITNESS FOR A PARTICULAR PURPOSE. You are solely responsible
for determining the appropriateness of using or redistributing the Work and assume any risks associated with Your
exercise of permissions under this License.
8. Limitation of Liability. In no event and under no legal theory, whether in tort (including negligence), contract, or
otherwise, unless required by applicable law (such as deliberate and grossly negligent acts) or agreed to in writing,
shall any Contributor be liable to You for damages, including any direct, indirect, special, incidental, or consequential
damages of any character arising as a result of this License or out of the use or inability to use the Work (including
but not limited to damages for loss of goodwill, work stoppage, computer failure or malfunction, or any and all other
commercial damages or losses), even if such Contributor has been advised of the possibility of such damages.
22 | ZOE Fluorescent Cell Imager Instruction Manual22 |
9. Accepting Warranty or Additional Liability. While redistributing the Work or Derivative Works thereof, You may
choose to offer, and charge a fee for, acceptance of support, warranty, indemnity, or other liability obligations and/or
rights consistent with this License. However, in accepting such obligations, You may act only on Your own behalf and
on Your sole responsibility, not on behalf of any other Contributor, and only if You agree to indemnify, defend, and hold
each Contributor harmless for any liability incurred by, or claims asserted against, such Contributor by reason of your
accepting any such warranty or additional liability.
Copyright © 2014, Bio-Rad Laboratories
All rights reserved.
Redistribution and use in source and binary forms, with or without modification, are permitted provided that the
following conditions are met:
1. Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer.
2. Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following
disclaimer in the documentation and/or other materials provided with the distribution.
THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS “AS IS” AND ANY
EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCL AIMED. IN NO EVENT SHALL THE
COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
EXEMPL ARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION)
HOWEVER CAUSED AND ON ANY THEORY OF LIABILIT Y, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT
(INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN
IF ADVISED OF THE POSSIBILIT Y OF SUCH DAMAGE.
The views and conclusions contained in the software and documentation are those of the authors and should not be
interpreted as representing official policies, either expressed or implied, of the FreeBSD Project.
| 23ZOE Fluorescent Cell Imager Instruction Manual | 23
24 | ZOE Fluorescent Cell Imager Instruction Manual24 |
Ordering Information
Catalog # Description
Instrumentation
145 - 0031 ZOE Fluorescent Cell Imager, 120–240 V, includes instrument, power supply, USB key,
instruction manual, quick guide
| 25ZOE Fluorescent Cell Imager Instruction Manual | 25
Bio-Rad
Laboratories, Inc.
Life Science
Group
Web site ww w.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 5 5 11 Brazil 55 11 3065 7550
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denma rk 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 9532 220 H ong Kong 852 2789 330 0 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 5 55 488 7670 The Netherlands 0318 540666
New Zealand 64 9 415 2280 Nor way 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04
Singapore 65 6415 3188 South Africa 27 861 246 723 Spain 34 91 590 52 00 Sweden 08 555 1270 0 Switzerland 026 674 55 05
Taiwan 886 2 2578 7189 Thailand 1800 88 22 8 8 United Kingdom 020 8328 2000
14-0981 0514 Sig 121310041704 Rev A US/EG
Loading...