Bio-Rad Unstained SDS-PAGE Standards User Manual

Ordering Information

Catalog Number Product Description

Molecular Weight Standards

161-0303 SDS-PAGE Standards, High, 200 µl 161-0304 SDS-PAGE Standards, Low, 200 µl 161-0317 SDS-PAGE Standards, Broad, 200 µl 161-0314 Silver Stain SDS-PAGE Standards, Low, 200 µl 161-0315 Silver Stain SDS-PAGE Standards, High, 200 µl 161-0306 Biotinylated SDS-PAGE Standards, Low, 250 µl 161-0311 Biotinylated SDS-PAGE Standards, High, 250 µl 161-0319 Biotinylated SDS-PAGE Standards, Broad, 250 µl 161-0320 2-D SDS-PAGE Standards 161-0326 Polypeptide SDS-PAGE Standards, 200 µl

Prestained Standards

161-0305 Prestained SDS-PAGE Standards, Low, 500 µl 161-0309 Prestained SDS-PAGE Standards, High, 500 µl 161-0318 Prestained SDS-PAGE Standards, Broad, 500 µl 161-0324 Kaleidoscope Prestained Standards, 500 µl 161-0325 Kaleidoscope Polypeptide Standards, 500 µl

IEF Standards 161-0310 IEF Standards, pI range 4.45-9.6, 250 µl

Silver Stain

SDS-PAGE Standards,

Low Range

Catalog Number

161-0314

Product shipped on dry ice.

Store at -20 °C upon arrival.

Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules CA 94547

4006047 Rev C

SDS-PAGE Molecular Weight Standards,Low Range Specifications

Low Range

Range Contents Rabbit muscle phosphorylase b

Volume 200 µl concentrated solution Storage -20 °C Shelf Life 1 year at -20 °C Applications 400 full size gels

per vial 800 mini gels Recommended 12.5%*

gel percentages

14,400 to 97,400 daltons

Bovine serum albumin Hen egg white ovalbumin Bovine carbonic anhydrase Soybean trypsin inhibitor Hen egg white lysozyme

*Note:

The lowest recommended gel percentage for the low range standards is 10%. At gel percentages of 10% or less, one or more of the standards may migrate at the dye front, depending on running conditions and other factors. As a result, only four or five bands may be visible after staining. If this should occur, an increase in gel percentage is recommended.

Silver Stain SDS-PAGE Standards contain approximately 700 µg total protein in 50% glycerol (w/v), 300 mM NaN3, 20 mM Tris, and 4 mM EDTA. The proteins have been blended to give bands of equal intensity on SDS polyacrylamide gel systems run according to Laemmli1and stained with Bio-Rad Silver Stain or Silver Stain Plus. Different results may be obtained when alternative silver staining chemistries are used.

Reference

1. Laemmli, U. K., Nature, 227, 680 (1970).

2. Hames, B. D. and Rickwood, D., Gel Electrophoresis of Pro- teins: A Practical Approach, Second Edition, p. 17, Oxford University Press, New York (1990).

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