Bio-Rad Unstained SDS-PAGE Standards User Manual

Ordering Information

Catalog
Number Product Description

Molecular Weight Standards

161-0303 SDS-PAGE Standards, High, 200 µl
161-0304 SDS-PAGE Standards, Low, 200 µl
161-0317 SDS-PAGE Standards, Broad, 200 µl
161-0314 Silver Stain SDS-PAGE Standards, Low, 200 µl
161-0315 Silver Stain SDS-PAGE Standards, High, 200 µl
161-0306 Biotinylated SDS-PAGE Standards, Low, 250 µl
161-0311 Biotinylated SDS-PAGE Standards, High, 250 µl
161-0319 Biotinylated SDS-PAGE Standards, Broad, 250 µl
161-0320 2-D SDS-PAGE Standards
161-0326 Polypeptide SDS-PAGE Standards, 200 µl

Prestained Standards

161-0305 Prestained SDS-PAGE Standards, Low, 500 µl
161-0309 Prestained SDS-PAGE Standards, High, 500 µl
161-0318 Prestained SDS-PAGE Standards, Broad, 500 µl
161-0324 Kaleidoscope Prestained Standards, 500 µl
161-0325 Kaleidoscope Polypeptide Standards, 500 µl

IEF Standards
161-0310 IEF Standards, pI range 4.45-9.6, 250 µl

Silver Stain

SDS-PAGE Standards,

Low Range

Catalog Number

161-0314

Product shipped on dry ice.

Store at -20 °C upon arrival.

Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules CA 94547

4006047 Rev C

SDS-PAGE Molecular Weight
Standards,Low Range
Specifications

Low Range

Range
Contents Rabbit muscle phosphorylase b

Volume 200 µl concentrated solution
Storage -20 °C
Shelf Life 1 year at -20 °C
Applications 400 full size gels

per vial 800 mini gels
Recommended 12.5%*

gel percentages

14,400 to 97,400 daltons

Bovine serum albumin
Hen egg white ovalbumin
Bovine carbonic anhydrase
Soybean trypsin inhibitor
Hen egg white lysozyme

*Note:

The lowest recommended gel percentage for the low
range standards is 10%. At gel percentages of 10%
or less, one or more of the standards may migrate at
the dye front, depending on running conditions and
other factors. As a result, only four or five bands
may be visible after staining. If this should occur, an
increase in gel percentage is recommended.

Silver Stain SDS-PAGE Standards contain approximately
700 µg total protein in 50% glycerol (w/v), 300 mM NaN3,
20 mM Tris, and 4 mM EDTA. The proteins have been
blended to give bands of equal intensity on SDS polyacry­lamide gel systems run according to Laemmli1and stained
with Bio-Rad Silver Stain or Silver Stain Plus. Different
results may be obtained when alternative silver staining
chemistries are used.

Reference

1. Laemmli, U. K., Nature, 227, 680 (1970).

2. Hames, B. D. and Rickwood, D., Gel Electrophoresis of Pro-
teins: A Practical Approach, Second Edition, p. 17, Oxford
University Press, New York (1990).

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