Bio-Rad UNOsphere Rapid S Media User Manual
Bio-Scale™ Mini
UNOsphere™ Q, S and Rapid S
Cartridges, 1 and 5 ml
Instruction Manual
Catalog #
732-4100, 732-4102, 732-4400,
732-4104, 732-4110, 732-4401,
732-4112, 732-4114, 732-4402
For technical support, contact your local Bio-Rad office, or in the
U.S., call 1-800-4BIORAD (1-800-424-6723).
Table of Contents
Section 1...Introduction. .......................................1
Section 2...Connection to Bio-Rad’s Low-
..............Pressure Chromatography
..............Instruments ........................................5
Section 3 ..Connection to Other Liquid
..............Chromatography Systems................10
..............3.1 BioLogic DuoFlow™
Systems.................................11
3.2 HPLC Systems ......................11
3.3 FPLC Systems .......................12
Section 4 ..Preparing a Cartridge For Use..........13
..................4.1 Sample Preparation ...............14
..................4.2 General Purification Protocol..15
..................4.3 Scaling Up the Separation .....16
Section 5 ..Care of the Cartridge........................19
..................5.1 Autoclaving ............................20
..................5.2 Storage..................................20
Section 6 ..Technical Assistance ........................21
Section 7 ..Ordering Information.........................22
Section 8 ..References .......................................24
Section 1
Introduction
Bio-Scale™ Mini cartridges have a patent-pending,
double-wall design that provides extra durability and
allows easy, reliable runs with aqueous buffers most
commonly used for protein purification. The
polypropylene Luer fittings and internal sealing
surfaces assure leak-free operation at pressures up
to 45 psi. The cartridges are convenient, disposable,
and supplied ready for use. Cartridges are available
for a variety of chromatographic techniques
including desalting, ion exchange, and affinity
chromatography. See Ordering Information Section 7
for a listing of the complete Bio-Scale Mini
cartridge product line. The design of the Bio-Scale
Mini cartridges offers:
1
• Ready-to-go convenience; simply equilibrate the
cartridge in the buffer of choice
• Luer fittings for convenient connection to any
chromatography system or directly to a Luer-Lok
syringe
The Bio-Scale Mini UNOsphere™ Q, S, and Rapid S
cartridges are packed with UNOsphere ion
exchange supports. These supports are based on
hydrophilic spherical, polymeric beads designed for
the purification of proteins, nucleic acids, viruses,
plasmids, and other macromolecules. UNOsphere
beads are designed to provide high capacity, low
back-pressure, and high productivity. Detailed
product information is given in Tables 1 and 2.
2
Table 1 Bio-Scale Mini UNOsphere Q, S, &
Rapid S Cartridge Specifications.
Sizes 1 and 5 ml bed volumes
Dimensions 1 ml: 40 x 5.6 mm inner diameter
5 ml: 40 x 12.6 mm inner inner diameter
Maximum pressure tolerance 45 psi
Recommended flow rates 1 ml: 1–2 ml/min (240–480 cm/hr)
5 ml: 5–10 ml/min (140–480 cm/hr)
Maximum flow rate 1 ml: 4 ml/min (970 cm/hr)
5 ml: 20 ml/min (963 cm/hr)
Fittings Female Luer fitting inlet and
Column material Polypropylene
Frit material Polyethylene (HDPE)
Shipping condition 20% ethanol
Storage recommendation 20% ethanol
Autoclavability Not autoclavable
male Luer fitting outlet
3
Table 2 UNOsphere Q, S, and rapid S
Specifications
UNOsphere Q UNOsphere S UNOsphere Rapid S
Type of ion exchanger Strong anion Strong cation Strong cation
Functional group -N+(CH
Total ionic capacity 120 µeq/ml Ni2+260 µeq/ml 140 µeq/ml
Dynamic binding capacity*
At 150 cm/hr 180 mg BSA/ml 60 mg IgG/ml 60 mg/ml
At 600 cm/hr 125 mg BSA/ml 30 mg IgG/ml 30 mg/ml
Shipping counterion Cl
Median particle size 120 µm 80 µm 100 µm
Recommended 50–1,200 cm/hr 50–1,200 cm/hr 50–800 cm/hr
linear flow rate
Chemical stability
1.0 M NaOH (20ºC) Up to 10,000 hr Up to 10,000 hr Up to 800 hr
1.0 M HCl (20ºC) Up to 200 hr Up to 200 hr Up to 200 hr
pH stability 1–14 1–14 1–14
Antimicrobial agent 20% ethanol 20% ethanol 20% ethanol
Regeneration 1–2 M NaCl 1–2 M NaCl 1–2 M NaCl
Storage conditions 20% ethanol or 20% ethanol or 20% ethanol or
* 10% breakthrough capacity determined with 4.5 mg/ml human IgG (UNOsphere
Rapid S) and 2.0 mg/ml BSA (UNOsphere Q) in a 1.1 x 20 cm column.
3)3
-
0.1 M NaOH 0.1 M NaOH 0.1 M NaOH
-SO
Na
3
+
4
-SO
Na
3
+
Section 2
Connecting to Bio-Rad's
Low-Pressure
Chromatography
Instruments
The Bio-Scale™ Mini cartridges are ideal for use with
Bio-Rad's BioLogic™ LP system, Econo™ Gradient
Pump, and Model EP-1 Econo pump, and all lowpressure chromatography instruments. Bio-Scale
Mini cartridges can be conveniently connected
directly to the system using the Luer lock fittings on
the cartridge.
1. Install 1.6 mm ID tubing in the pumphead.
Adjust platen pressure screw (on pumphead).
Using a screwdriver or coin, turn the screw
counterclockwise as far as it will go, then turn
clockwise three full turns. Assemble with fittings
and lock rings as shown in Figure 1.
5
(Use orange lock rings and medium size barb
fittings with 1.6 mm tubing.)
Platen pressure
screw
See detail
2 3
1
9
456
7 8
0
C .
Alarm
Fig. 1. Biologic LP setup
6
Lock ring
Tubing
Luer fitting
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