Bio-Rad UNO Monolith Cation Exchange Columns User Manual
UNO
™
Q&S R Continuous
Bed Ion Exchange
Replacement Column
Instruction Manual
Catalog Numbers
720-0011, 720-0013, 720-0015,
720-0031, 720-0033 and 720-0035
For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723)
Introduction
UNO prepacked ion-exchange columns are designed to meet the needs of the bio-chromatographer for rapid and reproducible high-resolution separations of biomolecules including proteins, peptides and polynucleotides. The UNO Replacement columns provide an
economical alternative to purchasing a new complete UNO column. The UNO Replacement
column hardware utilizes the existing hardware from your worn-out UNO column.
Installing the Replacement Column
Preparing the Used UNO Column (refer to Figure 1)
1. Remove the tube end fittings (2) from the UNO column end caps (3).
2. Remove one end cap (3) from the column and remove glass tube assembly (10) from the
clear shield (4).
3. Remove both tan bed supports (5) and spacers (7), if they are still attached, from the glass
tube and set aside.
4. Discard the glass tube filled with the used continuous bed support material in accordance
to local regulations.
Preparing the New UNO Replacement Column
1. Unthread the end caps (11) and remove UNO Replacement Column glass tube (16) from
the clear shield (12).
2. Remove the solid white plug (14) from both ends of the glass tube.
Assembling the New UNO Replacement Column
1. Each of the tan bed supports (5) from the used UNO column should have a 4 mm wide
brown spacer (7) located between the o-ring (6) and the top of the bed support. If the bed
support does not have the spacer, insert a new spacer, from the UNO Replacement col-
umn parts kit, onto the bed support (5).
2. Insert each tan bed support (5) with spacer (7) into each end of the UNO Replacement
Column glass tube (6). Push the bed supports into the column until they touch the frits (9).
3. Insert the UNO replacement column glass tube into the clear shield (4) and attach the
end caps (3) from the used UNO column onto the shield (4). Tighten the end caps.
4. Attach the tube end fittings (2) to the column and chromatography system and follow
equilibration protocol listed in the next section.
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