Bio-Rad Total Protein Blot Stains User Manual

SYPRO®Ruby Protein Stains

Instruction Manual

SYPRO®Ruby protein gel stain

Catalog Numbers

170-3125, 1 liter

170-3126, 200 ml

170-3138, 5 liter

SYPRO Ruby protein blot stain

Catalog Number

170-3127, 200 ml

SYPRO Ruby IEF stain

Catalog Number

170-3148, 400 ml

Table of Contents

PART 1: Introduction to SYPRO Ruby protein stains

Section 1 General Information.....................................................1

1.1 Introduction................................................................................1

1.2 Excitation and Emission ............................................................2

1.3 Storage .......................................................................................3

1.4 Materials Required But Not Supplied........................................3

1.5 Safety Considerations ................................................................3

1.6 Disposal Considerations.............................................................3

PART 2: SYPRO Ruby protein gel stain

Section 1 General Information.....................................................4

1.1 Introduction................................................................................4

1.2 Product Description ...................................................................4

Section 2 Instructions ...................................................................4

2.1 Staining ......................................................................................4

2.2 Viewing and Imaging a 1D or 2D Gel .......................................5

PART 3: SYPRO Ruby protein blot stain

Section 1 Introduction ..................................................................7

1.1 General Information...................................................................7

1.2 Product Description ...................................................................7

Section 2 Instructions ...................................................................7

2.1 Staining ......................................................................................7

2.2 Visualizing and Imaging a Blot .................................................9

PART 4: SYPRO Ruby IEF stain

Section 1 Introduction ................................................................10

1.1 General Information.................................................................10

1.2 Product Description .................................................................10

Section 2 Instructions .................................................................10

2.1 Staining ....................................................................................10

2.2 Viewing and Imaging an IEF Gel ............................................11

PART 1: Introduction to SYPRO Ruby
protein stains

Section 1
General Information

1.1 Introduction

The fluorescent stain, SYPRO Ruby protein stain is available in
three application specific formulations: 1) the SYPRO Ruby protein gel
stain for staining proteins in acrylamide gels (1-D, 2-D, isolectric
focusing); 2) the SYPRO Ruby protein blot stain for staining proteins
on nitrocellulose or PVDF membranes; 3) the SYPRO Ruby IEF
stain fo r st aining proteins in ac rylamide gels separated by isoelectric
focusing (IEF). These formulations have been optimized specifically for
each application and the stains are not interchangeable. Please refer
to the appropriate section of this manual for the stain you are using.

1

Introduction to SYPRO Ruby protein stains