Bio-Rad S Media EG-1 User Manual

Bio-Scale™ Nuvia™ S
Cartridges

1 and 5 ml

Instruction Manual

Catalog #732-4420
732-4421
732-4422
732-4423

Bio-Rad Technical Support

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Copyright © 2010 by Bio-Rad Laboratories, Inc. All rights reserved.

For help and technical advice, please contact the Bio-Rad Technical
Support department. In the United States, the Technical Support
department is open Monday–Friday, 5:00

Phone: [phone]

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(for U.S. and international customers)

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FPLC is a trademark of GE Healthcare Group Companies.
Luer-Lok is trademark of Becton, Dickinson and Company.

Contents

Section 1 Introduction........................... 1

Section 2 Connecting to Bio-Rad’s

Section 3 Connecting to Other Liquid

Section 4 Preparing a Cartridge for Use.........

Low-Pressure Chromatography

Instruments...........................5

Chromatography Systems ...........10

3.1 BioLogic DuoFlow™ Systems..........11

3.2 HPLC Systems .......................11

3.3 FPLC Systems .......................12

1 3

4.1 Sample Preparation...................14

4.2 General Purification Protocol..........15

4.3 Purification Scale-Up .................17

Section 5 Cleaning-in-Place (CIP) and

Section 6 Ordering Information................. 2 0

Section 7 Related Reading.................... .23

Sanitation............................18

5.1 Autoclaving........................... 19

5.2 Storage.............................. 1 9

Section 1 Introduction

Bio-Scale™ Mini cartridges have a patented*
double-wall design that provides extra
durability and allows easy, reliable runs with
the aqueous buffers most commonly used for
protein purification. The polypropylene luer
fittings and internal sealing surfaces ensure
leak-free operation at pressures up to 45 psi.
The cartridges are convenient, disposable,
and supplied ready for use.

Cartridges are available for a variety of
chromatographic techniques including
desalting, ion exchange, and affinity
chromatography. See Section 6, Ordering
Information, for the complete Bio-Scale Mini

cartridge product line.

*

U.S. patent 7,208,087.

1

The design of the Bio-Scale Mini cartridges
offers:

n

Convenience — the cartridge is ready for

equilibration in the buffer of choice

n

Versatility — luer fittings allow connection

to any chromatography system or directly
to a Luer-Lok syringe

Bio-Scale Mini Nuvia

™

S cartridges are
packed with Nuvia S ion exchange support.
This ultra-high capacity ion exchange
support is built on the UNOsphere

™

platform
and is designed for purification of proteins,
nucleic acids, viruses, plasmids, and other
macromolecules. Nuvia S support may be
used at any stage of the purification process.

2

Table 1. Bio -Sc ale Mi ni Nuv ia S cartr idge s peci ficat ion s.

Sizes 1 and 5 ml bed volumes
Dimensions 1 ml: 40 x 5.6 mm inner diameter (ID)

5 ml: 40 x 12.6 mm ID
Max pressure 45 psi

tolerance
Recommended 1 ml: 1–2 ml/min (240–280 cm/hr)

flow rates 5 ml: 5–10 ml/min (140– 480 cm/hr)
Max flow rate 1 ml: 4 ml/min (970 cm/hr)

5 ml: 20 ml/min (963 cm/hr)
Fittings Female luer fitting inlet

Male luer fitting outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Shipping conditions 20% ethanol + 0.1 M NaCl
Storage 20% ethanol or 0.1 N NaOH
Autoclavability Not autoclavable

3

Table 2. Nuvi a S medi a spec ifica tio ns.

Type of exchanger Strong cation
Functional group –SO

–

3

Total ionic capacity 90–150 µeq/ml
Dynamic binding capacity* ≥110 mg/ml (300 cm/hr)
Shipping counterion Na

+

Median particle size 85 ± 15 µm
Recommended linear flow rate** 50 –600 cm/hr
Chemical stability

1.0 N NaOH (20°C) Up to 1 week

0.1 N NaOH (20°C)

***

pH stability

2–14 short-term

***

Up to 5 yr

4–13 long-term
Shipping 20% ethanol + 0.1 M NaCl
Regeneration 1–2 M NaCl
Sanitation 0.5 –1.0 N NaOH
Storage 20% ethanol or 0.1 N NaOH

*

10% breakth rough capacity determined with 4.5 mg/ml human IgG in
40 mM NaOAc, 30 m M NaCl, pH 5.0.

**

Elution flow rates <150 cm/hr minimize viscosity-induced backpressure.

***

Data derived under accelerated conditions at 60ºC.

4

Section 2 Connecting to Bio-Rad’s Low-Pressure Chromatography Instruments

Bio-Scale™ Mini cartridges are ideal for use
with Bio-Rad’s BioLogic
gradient pump, Model EP-1 Econo pump, and
all low-pressure chromatography instruments.
They can be connected using the convenient
Luer-Lok fittings on the cartridge.

1. Install 1.6 mm ID tubing in the pumphead.

Adjust the platen pressure screw (on the
pumphead). Using a screwdriver or coin,
turn the screw counterclockwise as far as
it will go, then turn it clockwise three full

turns.

™

LP system, Econo™

5