Biochrom Ultrospec 1100 Pro User manual

Biochrom Ltd

Certificate No. 890333

Declaration of Conformity

This is to certify that the Ultrospec 1100 pro UV/Vis Spectrophotometer

Part number 80-2112-00 / 01 / 02 / 03
Serial number 79000 onwards

and the Ultrospec 500 pro Visible only Spectrophotometer

Part number 80-2112-50 / 51 / 52 / 53
Serial number 79000 onwards

manufactured by Biochrom Ltd. conform to the requirements of the following
Directives-: 73/23/EEC & 89/336/EEC

Standards to which conformity is declared

• EN 61 010-1: 1993 Safety requirements for electrical equipment for measurement,

control and laboratory use.

• EN 61326-2.3: 1998 Electromagnetic compatibility - Generic emission standard

part 1. Electrical equipment for measurement, control and laboratory use.

• EN 61000-4-6: 1992 Electromagnetic compatibility - Generic immunity standard

part 1. Residential, commercial and light industry.

Signed: Dated: 2nd April 2002

David Parr
Managing Director
Biochrom Ltd

Postal address Telephone Telefax

Biochrom Ltd [phone] [phone]
22 Cambridge Science Park
Milton Road e mail: [e-mail] website: http://www.biochrom.co.uk
Cambridge CB4 0FJ
England

Registered in England No: 974213
Registered Office: 22 Cambridge Science Park, Milton Road, Cambridge CB4 0FJ, England.

CONTENTS

Unpacking, Positioning and Installation 1
Essential Safety Notes 2

OPERATION 3

Introduction 3
Using the Instrument Display and Keypad 4
Customisation of the instrument menu 5
Basic Modes of Use 6
Enhanced Modes of Use 10
Method storage, recall and deletion 18

SET-UP 19

Menu customisation, access code and methods 19
Lamp settings 20
Display contrast and instrument output 20

ERROR MESSAGES 21

OUTPUT OF RESULTS 22

Use with parallel printer 22
Use with chart recorder 22
Use with PC 22

ACCESSORIES 23

Lamps, consumables and other items 23

MAINTENANCE 24

After Sales Support 24
Lamp Replacement 24
Deuterium Lamp Warranty (Ultrospec 1100 pro) 26
Fuse replacement 26
Cleaning and general care of the instrument 26

APPENDIX 27

Equation entry using the Multi Wavelength mode 27

SPECIFICATION 28

Warranty 30

Unpacking, Positioning and Installation

• Inspect the instrument for any signs of damage caused in transit. If any damage

is discovered, inform your supplier immediately. Check the position of the metal
lamp bracket inside the lamp access area.

• Ensure your proposed installation site conforms to the environmental conditions

for safe operation:

Indoor use only
Temperature 10°C to 40°C
Maximum relative humidity of 80 % up to 31°C decreasing linearly to 50 % at
40°C

• The instrument must be placed on a hard, flat bench or table that can take its

weight (6 kg) such that air is allowed to circulate freely around the instrument.

• Ensure that the cooling fan inlets and outlets are not obstructed; position at

least 2 inches from the wall.

• This equipment must be connected to the power supply with the power cord

supplied and MUST BE EARTHED (GROUNDED). It can be used on 90 - 240V
supplies.

• Switch on the instrument. Prior to calibration, the display asks you to check that

the cell compartment is clear. The purpose of this is to indicate the use of the
function soft keys, and how they are associated with the options presented at
the bottom of the display; F2 represents OK in this instance (this display can be
disabled in Set-up if required). The calibration stages are indicated in sequence
(- for checking, ü for OK, û for Fail).

• At switch on the language of the instrument can be changed if required. The

relevant key should be held down while the instrument is switched on. The
following numbers correspond to the languages available:

0 English 1 German 2 French 3 Spanish 4 Italian

If this equipment is used in a manner not specified or in environmental conditions not
appropriate for safe operation, the protection provided by the equipment may be
impaired and instrument warranty withdrawn.

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Issue 02 - 08/2002 English 1

Essential Safety Notes

There are a number of warning labels and symbols on your instrument. These are
there to inform you where potential danger exists or particular caution is required.
Before commencing installation, please take time to familiarise yourself with these
symbols and their meaning.

Caution (refer to accompanying documents).
Background colour yellow, symbol and outline black.

WARNING

UV RADIATION UV RADIATION IS HARMFUL TO YOUR EYES
HOT If power is restored with this cover removed,

eye protection must be worn

WARNING

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OPERATION

Introduction

Your spectrophotometer is a simple-to-use, microprocessor-controlled instrument. In
addition to the basic modes of operation, the instrument has enhanced software and
method storage functionality. A laboratory technician or supervisor can customise
the spectrophotometer for students and operators by disabling menu options that are
not required.

Your spectrophotometer:

• has basic modes of operation -
♦ measurement of absorbance, % transmission and concentration values,

♦ output of simple kinetics assays and wavelength scans to display.

• has enhanced modes of operation -
♦ the facility to enter a multi point standard curve in memory

♦ the application of a factor to an absorbance change over a specified time

interval for an enzymatic determination (reaction rate)

♦ the use of absorbance values in a multi wavelength equation specified by

you, with direct output of results, saving post run calculation

♦ the ability to quantify nucleic acids using stored equations and factors

(Ultrospec 1100 pro only)

• stores up to 9 user defined methods

• can have any combination of the above, including methods, enabled so as to

customise the instrument for your own specific laboratory needs

• can be connected to a standard Centronics parallel printer for output of results

• can be linked via a serial interface adapter lead to a PC for download of results to

spreadsheet, and subsequent inclusion in a laboratory information management
system (LIMS)

A range of accessories further enhances the capability of the instrument.

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Using the Instrument Display and Keypad

The back-lit liquid crystal display has large characters which are easily visible, useful
if a group of students are gathered around for a demonstration, for example. The
keypad is a spill proof membrane that is very hard wearing.

The instrument is easy to use, with function select / entry soft keys on the keypad
(F1, F2 and F3) being situated directly below the corresponding option on the
display; these keys are used in conjunction with on-screen prompts.

On the absorbance home page, for example, concise help text is available at the push
of the F1 key, whereas Menu and Set-up are accessed by F2 and F3, respectively. On
other displays, the purpose of the keys changes, but this is clearly indicated; for
example, F3 acts as the “accept” option on parameter entry displays and next page (if
further options exist) in the Menu and Set-up displays.

Pressing the red stop key acts as an escape mechanism in most situations.

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Press:

←← to clear incorrect user entries from the display.
λλ to enter the wavelength at which the instrument is to be used.

to set reference of absorbance to 0.000AU on a reference solution at the
current wavelength in the mode selected. Where it is standard operating
procedure, the user is prompted to insert a cell containing a reference into
the cell holder.

to start making measurements or print results.

to stop making measurements or return to the initial parameter screen within
the current operating mode.

• to print result

Esc to stop an experiment (time intervals and wavescan only)

OK To go to the absorbance page after calibration or from set-up.

Customisation of the instrument menu

Customisation of the menu to suit laboratory needs is an important benefit of the
instrument. This facility is password protected so that only authorised personnel are
able to set up or change the instrument.

• In a teaching laboratory, a laboratory technician might choose to have only the

modes of Absorbance, Factor Concentration, Time Intervals and Scan available.

• In the QC lab, the supervisor may choose to have Absorbance, Standard

Concentration, Multi-point Standard Curve entry and Reaction Rate.

• Similarly, a production line might have Absorbance and two methods as the

instrument start up; in this case, the methods could both be multi wavelength
equations involving factors, and set up in the analytical laboratory for use by
operators.

To customise the instrument, refer to Set-up > Menu for further details.

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Basic Modes of Use

Absorbance

Absorbance mode is default after power on and calibration. It makes simple
absorbance measurements on samples, measuring the amount of light that has
passed through a sample relative to a blank (this can be air). The procedure is as
follows:

Press λ key and enter appropriate wavelength
Insert reference, press key.

This reference value is used for subsequent samples until changed.
Insert samples as required and record the absorbance.

% Transmission

Transmission mode measures the amount of light that has passed through a sample
relative to a blank (this can be air), but displays the result as a percentage. The
procedure is as follows:

Press λ key and enter appropriate wavelength
Insert reference, press key

This reference value is used for subsequent samples until changed.
Insert samples as required and record the transmittance.

Concentration

Factor concentration mode is used when a conversion factor is known, and is
required to convert the absorbance measurement for a sample at a specific
wavelength into a concentration, by a simple multiplication of absorbance x factor.
The procedure is as follows:

Enter appropriate wavelength
Enter known factor (range 0.01-99999)

Insert reference, press key
This reference value is used for subsequent samples until changed.
If you wish to save as a method, go to set-up (F3)

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Insert samples as required and record the concentrations.

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Time Intervals

Simple kinetics studies for teaching laboratory experiments can be readily performed.
The wavelength of interest is entered together with the time interval at which
absorbances are to be read; the option of having a reference reading prior to the run
is available. A count down facility indicates the time remaining until the next
measurement. To end an experiment, the stop key is pressed. The procedure is as
follows:

Enter appropriate wavelength
Enter time unit (seconds or minutes)
Enter end time ( 10,000)
Enter the time interval for each reading (range 1-60 seconds). A minimum of 10 data
points are required, and the time interval for this is calculated.
If you require a reference reading press F3 (if not, press F2)

Insert reference, press key
This reference value is used for subsequent samples until changed.
If you wish to save as a method, go to set-up (F3)

Insert sample, press key
Press . to print graphic
Press key when the experiment is complete

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